LOCUS       NZ_CP083155             5535 bp    DNA     circular CON 11-APR-2024
DEFINITION  Bacillus thuringiensis strain GC-91 plasmid pCH_186-k, complete
            sequence.
ACCESSION   NZ_CP083155
VERSION     NZ_CP083155.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214333
            Assembly: GCF_020809205.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler, Canu v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x, 50x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809205.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 23:38:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,020
            CDSs (total)                      :: 6,865
            Genes (coding)                    :: 6,531
            CDSs (with protein)               :: 6,531
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 120 of 334
            Pseudo Genes (incomplete)         :: 222 of 334
            Pseudo Genes (internal stop)      :: 91 of 334
            Pseudo Genes (multiple problems)  :: 76 of 334
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083155.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="GC-91"
                     /isolation_source="Biopesticide Agree WP"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_186-k"
                     /collection_date="2017"
     gene            join(5350..5535,1..267)
                     /locus_tag="K8Z38_RS34895"
                     /old_locus_tag="K8Z38_34845"
     CDS             join(5350..5535,1..267)
                     /locus_tag="K8Z38_RS34895"
                     /old_locus_tag="K8Z38_34845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021728522.1"
                     /translation="MKKKEILEVTEMLSTFIDAGAGAQELMKAITERNLHKNSNFIFVF
                     HTLKAWIESDNPEVKLSKTEKAVLFTLLPYVGWNNQIELLQPTMQKLCGYKDRSYFSKV
                     LNSLVRKNVLEKQSVGKQNYWFFNSLLVKKGNSKERKPYIKFELKD"
                     /name="hypothetical protein"
     gene            859..1323
                     /locus_tag="K8Z38_RS34900"
                     /old_locus_tag="K8Z38_34855"
     CDS             859..1323
                     /locus_tag="K8Z38_RS34900"
                     /old_locus_tag="K8Z38_34855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011839882.1"
                     /translation="MLKIKSKFKKIIPATMAFAALLTVAPLSSFAAETETPVKNNVVNF
                     PQTNYKVENVNQTVSLDEDGDVSAQGWKKSAVVWGLKNGGDALQTLTKWLGAGTKEAKY
                     LNKHAKSIANALDRFESHIEQNLIDFMIFECGIPGGSARVIASAITGLVL"
                     /name="hypothetical protein"
     gene            1347..1745
                     /locus_tag="K8Z38_RS34905"
                     /old_locus_tag="K8Z38_34860"
     CDS             1347..1745
                     /locus_tag="K8Z38_RS34905"
                     /old_locus_tag="K8Z38_34860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1878 family protein"
                     /protein_id="WP_021728521.1"
                     /translation="MNIEQEISKLKYHKELMLTMLLHENPDKFSFYHQIINHDLEKKDE
                     IAILNIISLFNKRLSNENTFDFEKEFFDSISLQVIYDCNVKPTIEEYEGYLKAINIPIN
                     PKYLLMAINKQKESDNVCEFLLEKYKEK"
                     /name="DUF1878 family protein"
     gene            1792..2913
                     /locus_tag="K8Z38_RS34910"
                     /old_locus_tag="K8Z38_34865"
     CDS             1792..2913
                     /locus_tag="K8Z38_RS34910"
                     /old_locus_tag="K8Z38_34865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="WP_021728520.1"
                     /translation="MKKFIMIAVLLFGLCSGSVTSFASELSKKDDYLIMFRDKIDENLI
                     EKQGGEVKETYSNLPIVKAEFPYDPTKTLKQYFEVLEVEQDGNVKANGTKQSLHNGVSS
                     VLSTKGKGVSPLTGKGIAVAVLDTGIDREHPDVKIKDGVSFVENVLDFDDDNGHGTHLA
                     GIIGAQKNNSGMTGIAPDVDLYAVKVLDKYTNGKYSTVVKGIDWAIEHKANIILMSLGG
                     KKESVFFEEAINKAYEKGILLVSSAGNEGYKEGDSITYPAKFNSVIAVGALDAKDQRGF
                     LSSKGEQLELMAPGVDIWSTWNDGSYRFDSGTSMAAAHVAGVASLLLENDRMLSNQQVR
                     ELLKKSATPLGSPFEYGNGKINLSHALQMMNQK"
                     /name="S8 family peptidase"
     gene            3075..3371
                     /locus_tag="K8Z38_RS34915"
                     /old_locus_tag="K8Z38_34870"
     CDS             3075..3371
                     /locus_tag="K8Z38_RS34915"
                     /old_locus_tag="K8Z38_34870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021728519.1"
                     /translation="MAKEQIKLYVSEEEKERLELLAKMNSTTVSNYVRMTALGVRVRPP
                     KEIPVVVEKERLEDEDKLVLQELLERTQGKKDFITLDREFNTRLIALAGRLLE"
                     /name="hypothetical protein"
     gene            3777..5102
                     /gene="mobV"
                     /locus_tag="K8Z38_RS34920"
                     /old_locus_tag="K8Z38_34875"
     CDS             3777..5102
                     /gene="mobV"
                     /locus_tag="K8Z38_RS34920"
                     /old_locus_tag="K8Z38_34875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_021728523.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVDRGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMKKKVDAAVIIKSDYERLQKTDLVKENKELHS
                     AVDEICDSLKESQKRNVGLQKENKQLSTEISSLKAHIRDLQTNIKVLYQQTKQVFKEQF
                     KTFRGLVKNELVGREVENHFEREHERENKKKISRHRGYDMER"
                     /name="mobV"
     Typing          3778..5099
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.546"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaacagaga aagcagtttt atttactttg ctaccttatg tcggttggaa taatcaaatt
       61 gaacttttac aaccaactat gcaaaaacta tgtggttata aagataggtc atattttagt
      121 aaggttttaa attcattagt aaggaaaaat gttttagaaa aacaaagtgt aggaaaacaa
      181 aattattggt tttttaattc tttacttgtt aaaaaaggta attctaaaga acgaaaacca
      241 tatataaaat ttgaactaaa agactagtaa aaattactag tcttttttat ttacatattt
      301 tcccaatatg tggtttttac cccacatata tgtggttttt accccacata ttgggaatta
      361 attacaatcg ctagaacccc tgatataatc atgtttattc acttttttat taaaaattcc
      421 atgttgttct ttctatatct atataagggg ttggtttttc tccatacaat ggtcaggtag
      481 tattttttga aaaaaactct caaaaaatct ccacctttct tccattgcat tccaaaattc
      541 ggctggtttt tagcgttcgc caagccgaac agaccaaaaa cgggattaaa ttgctcgact
      601 tggactcgta attataccag ccgaaccacc aagttctatc gttccaagtg ataaacttat
      661 ccgataataa attacgttct tgggggctag cccccaaacc cctagccagc actcacaccc
      721 aaaaacaggg aaactcaaca ttggattact aattttgttt tccaaaagta tatgcaattt
      781 tacatataca gaaaaaagag ttttgaaata tgacatactt atctttgtta aaaaaattta
      841 tttctggagg aattatacat gttaaaaatt aaatctaaat tcaaaaaaat cattccagca
      901 acaatggcat ttgcggctct tttaacagtt gcacctttat cttcttttgc agctgaaaca
      961 gaaacgcctg taaaaaataa tgtagtgaat ttcccacaaa caaattataa agtagaaaat
     1021 gtaaatcaaa ctgtttcttt agatgaagat ggtgatgtat cagctcaagg gtggaaaaaa
     1081 tcagctgttg tttggggctt aaaaaatggt ggagatgcgt tacagacact tacaaaatgg
     1141 ttgggtgctg gaactaaaga agcaaaatat ttgaataaac atgcgaaaag tattgctaat
     1201 gcattagatc gttttgaaag tcatattgaa caaaatttaa ttgattttat gatttttgaa
     1261 tgcggaattc ctggtggatc agctcgagtt attgcaagtg caattactgg tcttgtttta
     1321 taattattga attgtaggtg tttaatatga atattgaaca agaaatttct aagctaaaat
     1381 atcataaaga attaatgtta actatgcttt tacatgaaaa ccctgataaa ttttcttttt
     1441 atcatcaaat tattaaccat gatttagaaa aaaaagatga gatagcaatt ttaaatatta
     1501 tttctttatt taacaaaagg ttatctaatg aaaatacatt tgattttgaa aaagaatttt
     1561 ttgattctat tagtttacaa gtaatttatg attgtaatgt aaaaccaact attgaggaat
     1621 atgaaggtta tttaaaggca ataaatattc cgataaaccc taaatattta ttaatggcaa
     1681 ttaataagca aaaagaatca gataatgttt gtgaattttt attagaaaaa tataaagaaa
     1741 aataaggtgc ttttgcacct tattttttat gtgtaatgga ggatcaaaaa tatgaaaaaa
     1801 tttattatga tagctgtatt gttatttgga ctatgcagtg gaagtgtaac ttcttttgcg
     1861 agtgagttat caaagaaaga tgattattta attatgtttc gagataaaat tgatgaaaat
     1921 ttgattgaaa aacaaggtgg ggaagtaaaa gaaacgtatt ctaatttacc cattgtaaaa
     1981 gctgaatttc cttatgatcc aacaaagact ttgaaacagt attttgaagt cttggaagtt
     2041 gaacaagatg gaaatgtaaa agcaaatggg acgaaacagt ctttacataa tggggtatcc
     2101 agtgttttat ctacgaaagg aaaaggagtt tcccctctta ctgggaaagg aattgctgtt
     2161 gcggttttag atactggtat tgatagagag cacccagatg taaagataaa agatggcgtt
     2221 tcatttgttg aaaatgtttt agattttgat gatgataatg gtcatggaac acatttagcg
     2281 ggtattattg gagcgcaaaa aaataatagc ggtatgacag gaattgctcc tgatgtggat
     2341 ttatatgcag tgaaagtatt agataagtat acgaatggaa aatatagtac agttgtaaaa
     2401 ggaatcgatt gggcaattga acataaggcg aatattattc taatgagtct aggtggaaaa
     2461 aaagaatctg tattttttga agaagccatt aataaagcat atgaaaaagg aattttgtta
     2521 gtttcatcag cagggaatga aggatataaa gagggagatt caattactta tccagctaaa
     2581 tttaattctg taattgcagt tggagcgtta gatgctaaag atcaacgagg gttcttatct
     2641 agtaaaggag aacagttaga attaatggca ccaggtgtag atatttggag tacttggaat
     2701 gatggttctt atagatttga tagtggtact tcaatggcag ccgctcatgt agctggtgtg
     2761 gcatctttgt tattagagaa tgatagaatg ttgtctaatc aacaagtaag agagttatta
     2821 aaaaaatcag caactccttt aggaagtcca tttgaatatg gaaatgggaa aataaattta
     2881 agtcatgctt tgcaaatgat gaatcaaaaa taaaagagaa ccaactttat agttggctct
     2941 cttttatttt ttaaaggggt atagaaggga taagtattaa agggtataaa aaggactata
     3001 aattatatat tgaaaaaggg tatataaagg gatatgatga aaggaacaaa atatacccta
     3061 agaggatgtt aaaaatggcg aaagaacaaa tcaaattata tgtttctgaa gaagaaaaag
     3121 aacgattaga gttattagct aaaatgaata gcacaacagt ttcgaactat gtaagaatga
     3181 cagctttagg tgttcgtgtt agaccaccta aagaaattcc tgtagttgtt gaaaaagaaa
     3241 gattagaaga tgaggacaaa ttggtgttac aggaattgct tgaacgaaca caaggaaaaa
     3301 aagattttat tacactagat agagaattta atacacgttt aattgcacta gctggacgat
     3361 tactcgaata aagaggaaat tttgaggtaa gtgtcaacgg taccggaccg agcccgaaag
     3421 ggatcgggag gataaggagt ggacacgtac gtcagatgta tcaccctctt ggcagttcct
     3481 caaaacatat tgttttgggg acgccaaccg gcgagggagc ccctcaagga ttgaggggtt
     3541 ggggagttcg atggatgggg tcaaggggaa aagttcccct tgtggggtgt ggggcaaagc
     3601 ccctagcatt tgtcacgcta tggcgtgatt tggcgaagcc aacaaggtct cgcagagccc
     3661 acaccaaatg taatttggta tagtgggcta taccaaaact ttggattcgc tctctcgata
     3721 tgttatgata accttaaaag tagtcctctt acggaagaca attgtgaggg aaaaacatga
     3781 acaaattcgc aatcattcac atgcagaaat ttcaaatttc tgatgtgcaa gggattcaaa
     3841 aacataatca aagacaagga aaaagtaaat caaatttaga catcgattat tcgaaaagtg
     3901 aacagaatta tgatttgttg aatcaacaaa aaattagata tgaaagcaca attaaacaag
     3961 agatcagtga gcgagtaaaa cgaaagccac gggcaaattc tgttgtgctt tcagaatttg
     4021 tagtaacagc ttcgcctgat tacatgcatt cattaagttt agaagaacag aaacgttatt
     4081 ttgagagttc actagatttt attcaaaagc gttatggcaa acaaaatacg ttgtatgcga
     4141 tggttcatat ggatgaagcg acaccacata tgcatattgg tgtgatgcca attacagaag
     4201 acaatcgttt gtccgcaaaa gatatgttta cgagaaaaga attgatatct ttgcaacagg
     4261 acttcccgtt ggaaatgcgg gaaaaaggtt ttgatgtaga tcggggagaa ggttctgaga
     4321 aaaaacattt atcgcctcaa gcctttaagg aaaaacaaga tttagaagta gaagtggaac
     4381 agttatccaa tgtaaaaacg catttaaaga caaaagtagt ggaaacgcat aatcaattac
     4441 aacaaaccac aaactacatc gaaaaacaga atgaaacgtt acaaaaaatt cagcaacaat
     4501 ttttgagttt agataaaaaa ataaaagaaa agaaacagga atttgaaacg tttcgaaatc
     4561 aaattccgga taagccagtg tcaatgtctt atttgcgaga agaaacaaaa acagaagtaa
     4621 caacgaaact gtttggaaag ccagaaatta ctgagaagaa aacaggaaac atcgtggtga
     4681 cacgtgagca atggagagat atgaaaaaaa aggttgatgc agcggttatt ataaagagcg
     4741 attatgaacg tttgcaaaaa acggatcttg taaaagaaaa caaagaatta catagtgcag
     4801 tcgatgagat atgtgactca cttaaagaat ctcaaaagag aaatgtaggg ttacagaagg
     4861 aaaacaaaca attaagtaca gaaataagct cattaaaagc tcatataaga gatttacaga
     4921 caaatataaa ggttttatat caacaaacga aacaagtctt taaagagcaa tttaagacgt
     4981 ttagagggct tgtaaaaaat gaattggttg gaagagaggt agaaaatcat tttgaacggg
     5041 agcatgaacg tgaaaataag aagaaaataa gtagacatcg agggtatgac atggagcgat
     5101 agaagacatt caaaaagtaa ataaaacttt tgggaaactt tatttgacaa tcttttgaga
     5161 gaatatgtgt gataaaatta aaaaacgagc catgctcgaa catggctcgt ttcaaagggt
     5221 atttcagcga aaataccaat aacttgttgt cgtatataga ttatcataat tttattatta
     5281 gggtaatacc tgaattatga tttttggtat ttttgttggt gctcggaata ttgagaggag
     5341 caacaacaaa tgaaaaagaa agagatttta gaagttacag agatgttgag tacttttatt
     5401 gacgctggtg caggtgctca agaacttatg aaggcgatca ctgaaagaaa tctacataag
     5461 aattcaaatt ttatatttgt ttttcatacg ttaaaagctt ggattgaaag tgataatcca
     5521 gaagtgaagc ttagt
//
