LOCUS       NZ_CP083154             8538 bp    DNA     circular CON 11-APR-2024
DEFINITION  Bacillus thuringiensis strain GC-91 plasmid pCH_186-j, complete
            sequence.
ACCESSION   NZ_CP083154
VERSION     NZ_CP083154.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214333
            Assembly: GCF_020809205.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler, Canu v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x, 50x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809205.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 23:38:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,020
            CDSs (total)                      :: 6,865
            Genes (coding)                    :: 6,531
            CDSs (with protein)               :: 6,531
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 120 of 334
            Pseudo Genes (incomplete)         :: 222 of 334
            Pseudo Genes (internal stop)      :: 91 of 334
            Pseudo Genes (multiple problems)  :: 76 of 334
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083154.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="GC-91"
                     /isolation_source="Biopesticide Agree WP"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_186-j"
                     /collection_date="2017"
     gene            join(8282..8538,1..763)
                     /locus_tag="K8Z38_RS34850"
                     /old_locus_tag="K8Z38_34795"
     CDS             join(8282..8538,1..763)
                     /locus_tag="K8Z38_RS34850"
                     /old_locus_tag="K8Z38_34795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3895 domain-containing protein"
                     /protein_id="WP_042991743.1"
                     /translation="MYGAYLDPEDRDRIMGSLSDEQQEYLRIFLKRGNQTAFSSVLARL
                     KGGESEKDAEHISTQWMFIEYIDAGKVSKDHPCECGRPLRYQYIVKNLVTNKILKFSKD
                     HFKKHTGLPSSIVKAVLKGMYKIDCELDEILIKISSGWSLFENLGMDSIPNDLIIPIDI
                     REHLELDLPLLERQVMRLKGDLRSYNSLKKNIVEKNDSVDFEINEEREEGQASLFDEDI
                     VFGFNLDDDNVQTIRSQPVKVKPVSIEKNISYQFLSFVEQEFILQFIQNVNSVSVRQLC
                     EFMIKEVGSLDKRYLTGKPHIYVHVCSYLDFLVTQGKLSYIENLDYIDRIYYIESDIS"
                     /name="DUF3895 domain-containing protein"
     gene            complement(795..935)
                     /locus_tag="K8Z38_RS34855"
                     /old_locus_tag="K8Z38_34800"
     CDS             complement(795..935)
                     /locus_tag="K8Z38_RS34855"
                     /old_locus_tag="K8Z38_34800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_170951690.1"
                     /translation="MTDKKERVEMRIPQSILKKVDEYKGENGISTRTATILELIRKGLN
                     K"
                     /name="hypothetical protein"
     gene            1046..1297
                     /locus_tag="K8Z38_RS34860"
                     /old_locus_tag="K8Z38_34805"
     CDS             1046..1297
                     /locus_tag="K8Z38_RS34860"
                     /old_locus_tag="K8Z38_34805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000888627.1"
                     /translation="MLEDKFLESKIKKLTNQNVVVFTDDEIIEGVLLDKDTGEERLFEV
                     FRKIWGKNFAWFGEGIYIQCEDTVTFRDYDEVDNIFKK"
                     /name="hypothetical protein"
     gene            3437..3970
                     /locus_tag="K8Z38_RS34865"
                     /old_locus_tag="K8Z38_34810"
     CDS             3437..3970
                     /locus_tag="K8Z38_RS34865"
                     /old_locus_tag="K8Z38_34810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_042991737.1"
                     /translation="MVVIYQTTKQAAQTLNINHTTFKKYYGMFERYNGYNFLRDSKGQV
                     MFSEYDLEVFKRLLSVKAEPGRTIEESCRVVGEEFGILNKNQVITDISPENGGDNKAIE
                     ELKELILMQNNKIDELTIKLNEQSNQTKMIETSVGDRDQQLVRLMKEMLEVKRMVAASN
                     KKRWWHFLKDKFSR"
                     /name="DUF3967 domain-containing protein"
     gene            4226..4531
                     /locus_tag="K8Z38_RS34870"
                     /old_locus_tag="K8Z38_34815"
     CDS             4226..4531
                     /locus_tag="K8Z38_RS34870"
                     /old_locus_tag="K8Z38_34815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042991738.1"
                     /translation="MENETIQEKNKRRRVPVRFSEITAQKLDDMSERYGMSVSALVNFI
                     VGQWLDNNYDIKDYTREKMIPAMMENVEPQMESMLQNPLIAGMFKEMAEQFVKASK"
                     /name="hypothetical protein"
     gene            4545..4787
                     /locus_tag="K8Z38_RS34875"
                     /old_locus_tag="K8Z38_34820"
     CDS             4545..4787
                     /locus_tag="K8Z38_RS34875"
                     /old_locus_tag="K8Z38_34820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042991740.1"
                     /translation="MEELLTKILEKLDAVDKKVDKVSGDVQELKQELKAHRMEIDDNFT
                     KLYKLVEDKDVEIDVLNKRVFKNETTVEKLKRQKH"
                     /name="hypothetical protein"
     gene            4858..5211
                     /locus_tag="K8Z38_RS34880"
                     /old_locus_tag="K8Z38_34825"
     CDS             4858..5211
                     /locus_tag="K8Z38_RS34880"
                     /old_locus_tag="K8Z38_34825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088065226.1"
                     /translation="MQNIKEIKKLIRKNPGKCASISTTEDGDFEGVLLSGFFAKQVMKL
                     ASQSNHISHDFNSNDLRSSDLNLKGVFLFNGTGVHFVFYEGLESIRIYDSPMFEEDSKE
                     MFEYFKEWLHAHS"
                     /name="hypothetical protein"
     gene            5312..5677
                     /locus_tag="K8Z38_RS34885"
                     /old_locus_tag="K8Z38_34830"
     CDS             5312..5677
                     /locus_tag="K8Z38_RS34885"
                     /old_locus_tag="K8Z38_34830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228236713.1"
                     /translation="MDTLWNCLERGVVMDTLWNCLERGVVMDTLWNCLERGVVMDLRTY
                     YENVINELESILLSSEKVCEDNFLKIEQYLSNKSFTENDIDYIFYHLDNLECLVKDRVF
                     LEDGLKRYREKLDKVKK"
                     /name="hypothetical protein"
     gene            5771..8143
                     /locus_tag="K8Z38_RS34890"
                     /old_locus_tag="K8Z38_34835"
     CDS             5771..8143
                     /locus_tag="K8Z38_RS34890"
                     /old_locus_tag="K8Z38_34835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_088065227.1"
                     /translation="MALDIAKCGRRTIALREKATGDVMIRRMSCNQLSCPVCGPVIAAR
                     LQLRIKYFSQHERLFFFNTITSKSGLDDLEKIFTKIRRELSVHFTIDGYMKKKKVSHEK
                     ALKWYEKKKKKMIDFDVDVELAIYSRKNAIIEVANSKKVFYKRLNVNQKIEFHKKYNHL
                     VDKLFPSIYETNKQDENLHKQLYERISRRFEENEFEDFKFIRVIELHESGQPHYHFLCN
                     RYVSHYLMKKITVEGVSEVYDNTYIVDHAMERNKILTYDTVDTDIVSFYVSKVTRYVTK
                     ETIETFSELEKEGDIAKKLISSSNCIKLFDEKDEEESKYKKLGVYDYQINATSYEVPKV
                     LENSVEDFIKFHSVMLRDQYAIDLLKFSKSLDLEDFIKCNYVIHERLIQVSVAEYKNKL
                     STVFDETDLTEEQLELLKSFVANKITLLLGRAGTGKTYTLVNLLKKLKPNPRTTFILTY
                     TGKASVRIKELLSQHELTEYKPMTVHKACSSNWNSNFLKNEYNNLECEYLIIDEVGMIP
                     RDILMKVLLAVPSYTKIIFAGDDAQLPPVNDSSVIPELEGLDFINTIRLTKVFRSGDTV
                     LELAYKVLNRELIDFRLYDEKQLEKLVLPLVEQGYQILTNTKKMTKKINLIVQANKDKI
                     TKCFNDYCYEIGDRVMIIANDSSRRVYNGDTGKIVDHDDKGILIQLDFEDRQVFYRYLD
                     TVNIVPAYAFTVHKSQGSEYENVAVFLESQPKLNTNNLLYTGITRAKKNIKVYVPDEKV
                     LSTMINCVPEPVSNITLNNVFDLVNGILDIKKTSSMG"
                     /name="AAA family ATPase"
ORIGIN
        1 tcaatatatt gttaagaatt tagtgactaa taaaatttta aagtttagta aggatcactt
       61 taaaaaacat acaggtttac cttctagtat tgttaaggca gttttgaagg gaatgtataa
      121 gattgattgt gagttagatg aaattttgat taaaatttca tctggttggt ctttatttga
      181 gaacttaggt atggattcta ttcctaatga tttgattatc cctatagata ttagagagca
      241 tttagaatta gatttacctt tattagaacg tcaggttatg agattaaaag gtgatttgag
      301 aagttataat tcattaaaga agaatattgt tgagaaaaat gattctgtag attttgaaat
      361 aaatgaggaa agagaagaag gacaagcttc tttgtttgat gaagacattg tttttggatt
      421 taatttagat gatgataatg ttcaaactat acgaagtcag cctgtgaaag tgaaacctgt
      481 ttctattgag aagaacattt cgtatcaatt tttaagtttt gttgaacaag aatttatttt
      541 gcagtttata caaaatgtta attctgtaag tgtgagacaa ctatgtgaat ttatgattaa
      601 agaggttggc tctttagata aacgttatct tactggaaag cctcatatct acgttcacgt
      661 ttgtagttat ttagattttt tagtcactca aggcaagttg agctatattg agaatttaga
      721 ttatattgat cgtatttatt atatagaatc agatattagt tagaagggat atgtatatta
      781 tcccttctat ttttttattt atttaatcct tttcttataa gttctagaat tgttgctgtt
      841 ctagtagaaa ttccgttttc tcctttgtat tcgtcaactt tttttaatat tgattgtggg
      901 attctcattt ctactctttc ttttttatct gtcattatta tcacctctcc ttgtattgta
      961 cggaaaaata tcgtataata caaattgtac ggaaaaaaga cgtattttta acgtacaaaa
     1021 agtgaaaatc aaaggggatt ttattatgtt agaagacaaa tttttagaat ctaagattaa
     1081 aaaattaact aatcaaaatg tagtggtttt tactgatgat gagattattg aaggagtcct
     1141 tttggataag gatactggag aagaacgttt gtttgaagtt tttcgtaaga tttggggtaa
     1201 gaattttgct tggtttggag aaggaattta tattcaatgt gaagatacag tgacgtttcg
     1261 tgattatgat gaggtagata atatatttaa gaaatagttc tatataaatt tatattaaat
     1321 gcagattatt tatagaagag cataaggttt ttttgctatc actaaaggtg acaggttaga
     1381 caaagcctaa caaaaacgtg atatcataaa tataagaaaa tcaatggagt ttcaatgtgt
     1441 ttccaatggg ttttcactgt atataatatg ctattggaaa ttgttttttt tgcttttaga
     1501 ttgataattt ttgtaaaaga taaaatgaaa ttccatttat aaatgttgta tattttaata
     1561 gttttatagg agtttttaag aagaggttga tgaggatcaa tgcacagcag ccatgtgcat
     1621 tattgtatta gcgaataaaa aaacgatatg ttaacgttac ccaaaaggat agctttaaca
     1681 tatcgttttt ttttattttt atgcctacta cggtaaggct acaccaattt tttagttcca
     1741 actaaggtat ggatacttca aaattaatca tcattctttt gttttatgtt gttgcgaagg
     1801 tgaacagaaa aaaacttcgt tgaatttcgt ttttatttta tcacaaattc aattttttgg
     1861 tgcattttta gtgaccacta agtgccacca aatgaccatc agaatgaaaa aatgaccacc
     1921 cagtgaccac caattaataa ataggcgtcc cagttttcgt ggtcgtttag tggtcatttt
     1981 tggcgcatac aaaacgcatg cttttaattt ttttcacata caaaacgcat ataaaatgac
     2041 caaaaacgca tacagaacgc atatgagtaa aaaaaagacg tcccactttt ttgggttaaa
     2101 aaaacgttga tttaaagggg cgtaaagcgt gctacaaggg acacaaaacg tgccacaaaa
     2161 gtgctttaaa cgtacacaaa acgggctaca aagcccaaaa aggcatcccg caattcacaa
     2221 actagaaacc caataggctc agcctatttc accacttttt ttataaagtg ggattcggtt
     2281 tccttatgct cttctttaaa aaaggtttat atggcttttt aagctggtaa atattttgta
     2341 ttaaaaaaag aaaatgcagg ataaggagag ggatacagtt gtgtctacgt gtatcagtca
     2401 aagcctgtta gaataatttt tcattgtgta cagggttgtg accacaattg aaaaaggtaa
     2461 tacgttttgt ggttacaaaa tatttataag gtctatgtta ctaatgtttt gatgtagcat
     2521 tactaataga tccgattgat taaataatta atgtttgtgg taattagcga ggaatagcga
     2581 agattaggga agacagtttt tgaagtatat tggagcgaag catgaagatt tttatacaag
     2641 taattgttat aaaaaaatca aaatgaaatg gcagcaggac taaattcagg gtagttggag
     2701 gtttaaatac ggatatagtg cttgggtttc aagaagagat aagtatatat ttagagattc
     2761 tatagaaaaa atgtttttaa tatttgtcgc agaattagtg cttacgccct agaggttatg
     2821 ttaggtatgg cgagcattcg gccttaggcg gatgcgcgcc agggatagtt aagcgttaag
     2881 aggatgaaaa atattaacaa ggtagttatc tatagaggta tttaatatat agttggattt
     2941 aattgtgagt tatagaagat atagccgata taacttcagg tataaaggta gaatgtttta
     3001 aaagaaggaa cgatgtatat attcatcaga gtacgcaaaa tcacttctga aaggtttgaa
     3061 atgtgaatcg gtagatggga atggaaagcc tgtaagaagc cgtagcaagg cgaaggagtt
     3121 ttatttagaa aagttttttg gtttgtaaaa agttttttaa ataaaagttg atgtcccgta
     3181 gggttgccta aaggaatagt aaaccaagct attgcgtact cccaaacatt gtttggggga
     3241 cacccgctgt gagcgtaaag ggtgtgggaa tatcccacat ataaaaaatt tttgtgtccc
     3301 tttttttaaa aaggacggtt taattttgtg aaaaatacgg gacgtgaaat ttcattgtga
     3361 tttataaagt ttatataaat tataaaacaa ggagttatat cggttatatc gaatgtaaat
     3421 ttgtgaggtg attgcaatgg ttgttatata tcaaacaaca aaacaagcag cacaaacatt
     3481 aaatattaat catacaactt ttaaaaaata ttatggtatg tttgagagat ataacggata
     3541 taacttctta agagattcaa aaggacaggt aatgtttagt gaatatgatt tagaagtatt
     3601 taagaggtta ttatcagtta aggcggaacc aggaaggact atagaagaat cttgtagagt
     3661 tgttggtgaa gagtttggta ttttgaataa gaatcaagtt ataaccgata tatctcctga
     3721 aaatggaggg gataataagg cgattgaaga gttaaaggaa ttgattttaa tgcagaataa
     3781 taaaatcgat gaattaacta ttaaattaaa tgaacaatca aatcaaacta agatgattga
     3841 aacaagcgtt ggagatagag atcagcaatt agttagatta atgaaagaaa tgttagaagt
     3901 gaaaagaatg gtggcagcaa gtaataaaaa aagatggtgg cattttttaa aagataaatt
     3961 tagccgataa ggcttttttt atttgttttt tttgttaaat atttcttgtt tatatgctat
     4021 ttttaagata atattagtaa cagaaagaaa aaatagtggt gtcattttgg attcattatt
     4081 gtattgtata tacaatacaa tagcaccgta tatacaaaat ctctttttta atatgtaaat
     4141 tatgtttttg catatgcgaa ttgcactttt acatattaga tttgtggatt ttagtattag
     4201 tttttgtaat aaggaggctt tttttatgga aaatgaaaca attcaagaaa agaataagag
     4261 gagacgtgtc cctgttaggt tttctgaaat tacggcacaa aagttagatg atatgtctga
     4321 gcgctacgga atgtcagtga gtgctttggt gaattttatt gttggtcagt ggttagacaa
     4381 taattatgat attaaagatt acactagaga aaaaatgata ccggcaatga tggaaaatgt
     4441 agaacctcaa atggaatcta tgcttcaaaa tccattgata gctggaatgt tcaaggaaat
     4501 ggctgaacaa tttgttaagg cttctaaata aaggagggat ttgtatggaa gaattattaa
     4561 cgaaaattct agaaaaacta gatgctgttg ataaaaaggt agataaagta agtggtgatg
     4621 tgcaggaatt aaaacaagaa ttaaaagcac atcgaatgga aatagatgat aattttacga
     4681 agttatataa gttagttgag gataaagatg ttgagattga tgttttgaat aaacgtgtat
     4741 ttaaaaatga aacaacagtt gaaaaattaa agagacaaaa acattaggat ttatatgtga
     4801 attaaaatta tatgtgctta tttttaaaga aagaagatat ttaaggggaa tgatatcttg
     4861 caaaatatta aagagataaa gaaattaatt aggaagaatc ctggtaaatg tgcaagcatc
     4921 tctacaactg aagatggtga ttttgaaggg gttttattaa gtggattttt tgcaaagcaa
     4981 gttatgaaat tggcttctca atctaatcat atttctcatg attttaattc aaatgatctt
     5041 agatcttctg atctaaattt aaaaggagtt tttcttttta atggtactgg agttcatttt
     5101 gttttttatg agggtcttga atctataagg atttatgatt cgccaatgtt tgaagaggat
     5161 tctaaagaga tgtttgagta ttttaaagag tggttacatg cacattcttg atatttactt
     5221 tagtaaacgt tgtaagaatg taataaaaca tatttaacag tttactgtgt aaatgggcac
     5281 tttgtggaat tgcttagaga gaggtgttgt tatggacact ttgtggaatt gcttagagag
     5341 aggtgttgtt atggacactt tgtggaattg cttagagaga ggtgttgtta tggacacttt
     5401 gtggaattgc ttagagagag gtgttgttat ggatttaaga acgtattatg agaatgttat
     5461 taatgaatta gaaagtattt tattgtcttc agaaaaggtt tgtgaagaca attttttaaa
     5521 gattgaacaa tatttaagta ataaatcttt tactgaaaat gatattgact atatttttta
     5581 tcatttagat aatcttgagt gtttagtaaa agatcgtgtt tttcttgaag atggtttaaa
     5641 aagatataga gaaaagttag ataaagtaaa gaagtaaatt cataatttaa tgtagtttct
     5701 acttaactag ttaataataa taggggtaga gttccacttt ttgattagct tcttggaaag
     5761 gttgtctttt atggctttgg atattgctaa atgtggtcgg cggaccattg ctctacgtga
     5821 aaaagctact ggtgatgtta tgatccgaag aatgtcctgt aatcagctct cctgtcctgt
     5881 atgtggtccg gtcattgcag cgcgtttaca acttcgaatt aaatattttt ctcagcatga
     5941 acgtttgttt ttctttaata ctattacttc taagagtgga ttggatgatt tagaaaagat
     6001 tttcactaag atacgtcggg agttatcagt acattttact attgatggat atatgaagaa
     6061 aaagaaagtt agtcatgaga aagctttaaa atggtatgaa aagaaaaaga aaaaaatgat
     6121 agattttgac gttgatgttg aacttgcaat ttattcaaga aaaaatgcaa ttattgaagt
     6181 tgcaaattca aaaaaggttt tttacaagcg tttaaatgtc aatcaaaaaa ttgaatttca
     6241 taagaaatat aatcatttag tagacaaatt atttccatct atttatgaaa ctaataagca
     6301 agatgaaaat ttacataaac agttgtatga acgaatttca agacgttttg aagagaatga
     6361 atttgaagat tttaagttta ttagggtaat tgagttacat gaaagtggac aacctcatta
     6421 tcatttttta tgtaatcgtt atgtttctca ttatttaatg aagaagataa cagttgaagg
     6481 tgtgagtgag gtttatgata atacgtatat tgtggatcat gctatggaga gaaataaaat
     6541 tcttacatat gacactgtag atactgatat tgtttctttt tatgtatcga aagttactag
     6601 atatgttact aaggagacta tagaaacctt ttctgaattg gaaaaggaag gtgatatagc
     6661 taagaaatta attagttcaa gtaattgtat caagttgttt gatgaaaaag atgaagaaga
     6721 atccaagtat aagaaattag gagtatatga ttatcaaatt aatgcaacaa gttatgaggt
     6781 tccaaaagta ttagaaaata gtgtagaaga ttttattaag tttcattctg taatgcttag
     6841 ggatcagtat gcaattgatt tattgaaatt ttcaaagtca ttggatttag aggattttat
     6901 taagtgtaat tatgtaattc atgagcgctt aattcaagtt tcagttgctg aatataaaaa
     6961 taagttatcg actgtttttg atgagactga tttaactgaa gaacaattag agttattaaa
     7021 atcttttgta gcaaataaaa tcacattatt gttaggacga gctggtacag gtaaaactta
     7081 tactttagtt aatttgttga aaaaattaaa gcctaatcca cgtacaactt ttattttaac
     7141 gtatacaggt aaagcgtctg tacgaattaa agaattgttg agtcaacatg aattaaccga
     7201 atacaagcct atgacagttc ataaagcatg ttcatctaat tggaattcga attttttaaa
     7261 gaatgagtat aataacttag aatgtgagta tttgattatt gatgaagtag gtatgatacc
     7321 aagagatatt ttgatgaaag tattattagc tgttcctagt tatacaaaga ttatttttgc
     7381 tggtgatgat gctcaacttc cacctgttaa tgattcttct gtaattcctg aattagaagg
     7441 tttagatttt ataaatacta taagacttac aaaagttttt agaagtggag atactgtgtt
     7501 agagcttgca tataaagttt taaatcgtga attaattgat tttagattat atgatgaaaa
     7561 acaattagaa aaactagttt taccacttgt ggaacagggg tatcaaattt taacaaatac
     7621 aaaaaaaatg actaagaaaa ttaatttaat agttcaagct aataaggata aaattacgaa
     7681 gtgttttaat gattattgtt atgaaatcgg tgatagagtc atgatcattg ctaatgattc
     7741 atctagaagg gtttataatg gtgatactgg taaaattgtt gatcatgatg ataaaggtat
     7801 tttaattcaa ttggattttg aagataggca agtgttttat agatatttag atacagttaa
     7861 tattgtacca gcttatgcat ttacagttca taaatctcaa ggtagtgaat atgagaatgt
     7921 ggcggtattt ttagaaagtc agccgaaatt aaatactaat aatttattat atacaggaat
     7981 tacaagagcg aagaaaaata taaaagttta tgtgccggat gaaaaggttt tatcgactat
     8041 gataaattgt gttccagaac cggtttcgaa tataacatta aataatgttt ttgatttagt
     8101 aaatgggatt ttagatataa aaaaaacatc ttcaatgggc tgaagatgta caaaaatgta
     8161 gattcttcaa tgggttgaag aatcataaga ctgaaatgat tgttaaagaa tctaattgta
     8221 ttatatatgt aatttgtagc atggtctatt taatttaatt tttcggaagg atttgttgtt
     8281 tatgtatggt gcttatttag atccagaaga tagggataga ataatgggta gtttgtctga
     8341 tgaacaacaa gagtatttac gtattttttt gaagagaggt aatcagacag cattctctag
     8401 tgtgttagct agattaaaag gtggcgaatc tgaaaaggat gctgaacata tatctactca
     8461 atggatgttt attgagtata ttgatgcagg aaaagtttcg aaagatcatc cgtgtgagtg
     8521 tggtcgtcct ttacgtta
//
