LOCUS       NZ_CP083153            10657 bp    DNA     circular CON 11-APR-2024
DEFINITION  Bacillus thuringiensis strain GC-91 plasmid pCH_186-i, complete
            sequence.
ACCESSION   NZ_CP083153
VERSION     NZ_CP083153.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214333
            Assembly: GCF_020809205.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler, Canu v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x, 50x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809205.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 23:38:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,020
            CDSs (total)                      :: 6,865
            Genes (coding)                    :: 6,531
            CDSs (with protein)               :: 6,531
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 334
            CDSs (without protein)            :: 334
            Pseudo Genes (ambiguous residues) :: 0 of 334
            Pseudo Genes (frameshifted)       :: 120 of 334
            Pseudo Genes (incomplete)         :: 222 of 334
            Pseudo Genes (internal stop)      :: 91 of 334
            Pseudo Genes (multiple problems)  :: 76 of 334
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083153.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="GC-91"
                     /isolation_source="Biopesticide Agree WP"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_186-i"
                     /collection_date="2017"
     gene            complement(181..444)
                     /locus_tag="K8Z38_RS34795"
                     /old_locus_tag="K8Z38_34740"
     CDS             complement(181..444)
                     /locus_tag="K8Z38_RS34795"
                     /old_locus_tag="K8Z38_34740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874968.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPSELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(458..745)
                     /locus_tag="K8Z38_RS34800"
                     /old_locus_tag="K8Z38_34745"
     CDS             complement(458..745)
                     /locus_tag="K8Z38_RS34800"
                     /old_locus_tag="K8Z38_34745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000332102.1"
                     /translation="MAYVISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            1104..1331
                     /locus_tag="K8Z38_RS34805"
                     /old_locus_tag="K8Z38_34750"
     CDS             1104..1331
                     /locus_tag="K8Z38_RS34805"
                     /old_locus_tag="K8Z38_34750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2653..3222
                     /locus_tag="K8Z38_RS34810"
                     /old_locus_tag="K8Z38_34755"
     CDS             2653..3222
                     /locus_tag="K8Z38_RS34810"
                     /old_locus_tag="K8Z38_34755"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_021727495.1"
                     /translation="MKVFEVLESLKKGEKLKEIANVLGINNSTLQRKLKALGYAFDSST
                     KEWFYNDKDNPEPLEENIMDMVKRNSKSGVNDLPKIIQGIKSETKGELGVKHGNTVKVS
                     EVLTSEDIADLHEMLLFWRTDRERTKKHKTLHEEIKTLRRDDRTRKTVILSKEVAKRLD
                     KLSERERFDKSDLLELALEHFLSEYE"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            complement(3575..4159)
                     /locus_tag="K8Z38_RS34815"
                     /old_locus_tag="K8Z38_34760"
     CDS             complement(3575..4159)
                     /locus_tag="K8Z38_RS34815"
                     /old_locus_tag="K8Z38_34760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021727497.1"
                     /translation="MKNRYEHLKNGYTAIFFKDSNNKETAFFIDTDCFEIANSHDFYWE
                     NYLVNSQGKRMRYNIIGRKKGESSKTQLSRLLLECNTQKEACYNITGNPLDLRKCNLLK
                     TNMGKVKTKEFQEEMNKLIKTVPPLPQIQLKNINEKEKDVAISPKNDNIRLLTDEQNNT
                     YILLENGIITAEIEKKYAKFLVKQLSHSITT"
                     /name="hypothetical protein"
     gene            4757..6031
                     /gene="ltrA"
                     /locus_tag="K8Z38_RS34820"
                     /old_locus_tag="K8Z38_34765"
     CDS             4757..6031
                     /gene="ltrA"
                     /locus_tag="K8Z38_RS34820"
                     /old_locus_tag="K8Z38_34765"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421374.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_021727498.1"
                     /translation="MELLEAILSNKNMNEAYLRVYKNKGVSGVDGVTVEELKQYLKEHK
                     DELRQRIRTRKYQPQAALRVEIPKENGKMRKLGIPTVVDRVVQQAIHQILSPIFEKQFS
                     EFSYGFRPKRSCEMAIVKSLEFLNNGYDWIVDIDLERFFDTVHHDKLMRIISNSIHDGD
                     VISLIRKYLVSGVMVNGKYEETPVGTPQGGNLSPLLSNIMLNELDKELEGRGLQFVRYA
                     DDALIFVRSEKAAGRVMKSIVRFIEKKLGLIVNTEKSKISRPKDLKFLGFGYYYTLKDK
                     RYQTKPHPISVQKFQRKLHQLTKRSWSVPLDYRILKLKQVIFGWVNYFRSTNMKGVLER
                     VDQKLRARIRVIIWKQWKVPKKQIKSLVQLGIPEEEAKGLTYCRKGFRYIGLSKVVQRA
                     ISNQRLKKRGFPSSLERYLKVHTVI"
                     /name="ltrA"
     gene            complement(6112..6513)
                     /locus_tag="K8Z38_RS34825"
                     /old_locus_tag="K8Z38_34770"
     CDS             complement(6112..6513)
                     /locus_tag="K8Z38_RS34825"
                     /old_locus_tag="K8Z38_34770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_021727499.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKISEQEASD
                     FTVNVVEQRLSFDEILNVIKENSSKLPSN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(6551..6835)
                     /locus_tag="K8Z38_RS34830"
                     /old_locus_tag="K8Z38_34775"
     CDS             complement(6551..6835)
                     /locus_tag="K8Z38_RS34830"
                     /old_locus_tag="K8Z38_34775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_021727500.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEGLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            7423..7995
                     /locus_tag="K8Z38_RS34835"
                     /old_locus_tag="K8Z38_34780"
     CDS             7423..7995
                     /locus_tag="K8Z38_RS34835"
                     /old_locus_tag="K8Z38_34780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097509.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_011279306.1"
                     /translation="MSKKEHLIDVQPIRSKEQLQDMKWALKRHCTDRDYILFVIGINTG
                     LRVSDLLNLETSMILELKKKRRKELKIREGKTKKERMVNITSIFEEVYLYAAGLESKWL
                     FPSRKGDRPISKIQVYRQLQKAGDFAGVESIGTHTMRKTFGYWFYKQTKDVAMLQEILN
                     HSAPKITLKYIGINKEEKDNVLDTFCL"
                     /name="tyrosine-type recombinase/integrase"
     gene            8765..9808
                     /locus_tag="K8Z38_RS34840"
                     /old_locus_tag="K8Z38_34785"
     CDS             8765..9808
                     /locus_tag="K8Z38_RS34840"
                     /old_locus_tag="K8Z38_34785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_021727501.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIRIYEFIEKKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKGEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKSYFKKKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYAG
                     LLKDYAKKYENGELDKYKSIDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGHL
                     IDEMEVE"
                     /name="protein rep"
     gene            9811..9978
                     /locus_tag="K8Z38_RS34845"
                     /old_locus_tag="K8Z38_34790"
     CDS             9811..9978
                     /locus_tag="K8Z38_RS34845"
                     /old_locus_tag="K8Z38_34790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011279307.1"
                     /translation="MYKKGDIVKHTCWECYNEEMKIYRISKSKVKDGEDVYYSYKCWVK
                     CEKCGAVDDV"
                     /name="hypothetical protein"
     Typing          8642..9808
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.377"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttttactct taaagtctgt ttgctttggt tcgcaaatga aaaaatcaaa aagtcacgca
       61 taacttttat taaatatata aatatattat taaaacacct aacccttttg tgatgaaaaa
      121 atataaatag ttaaagaaaa aagactgaca aaaatatcag tctttttttc ttgtttttag
      181 ttatagagag tatcttttta tggtattttc tactttttct ctccagtttt ctatatcgtc
      241 aattgatatt ccaatcttta ttaattcgct tggaatgaaa ctgttatcta aaatccataa
      301 agtgagcctt tctaatgtga ttctttcgtt tccaatcgaa taaatacgtt cactttgttt
      361 gcaggtatat ggttttttac caacatattt ttctacataa aagtatcttt ttccgtttgc
      421 tttgtaattt gttagaaatg ccatgttaat gctcctttca ggtattttta tggttagtta
      481 aagagtagtt ctgtaatgtt ttttcaactt tttcatacca atatttcaca tcgtttatgg
      541 atactccgat ttctagtagt tcttttggta tacggttgtt atctaatagc cacattgcga
      601 ttctttctaa agctattttt tgattgccga ttgcgtaaat gcatttatat ttgtattgct
      661 ttgagtaata tggttgtgtt ccaacgtatt gagctacata aaagtaaatg tttcctttta
      721 ctttttttga tattacatac gccaaaataa tgacctccaa ttattaaaat tctatatcac
      781 attgattaat attaacttta ttaggttaat ttttcaacaa ataaagtatg aaataatgca
      841 tattttgaac atttttattt aaataggttt taaattagtt taaatgaacg caataaataa
      901 ccttatatgt atagaaatta attttcgttt gaatgaagta tgaaataatg cataatgtga
      961 ttttttattc catacaggtt taaaaatgtt ttataaagtg ctaaagttgt gtttaaaagg
     1021 ttaaaaaaca actattattt gcacaaaagt gcctttataa gaaaaatagt atgtatattt
     1081 catgagaaaa aggagtataa aaaatgaaag tagtaaataa cttccgtgtg attatggcgg
     1141 taaagaatat agataatgtt gcagagttgg ttaggatgac aggggtaagt cgtaactcta
     1201 tcaataagtt gtggcgtaat gataatgtgt cgtctttaag aatagataca ttgttaccga
     1261 tctgtgaaaa gttaaatgtt aaactatcgg atttaattga atatattcca gatgatagtg
     1321 agacaaaata atttaggggc tattctatct ttagatagct tgtgtatgat gaaatcggta
     1381 ttaatatttg ctagtggaaa acaggttata ttcgcatatg gtttttggta gggttatatg
     1441 cgtattttaa atttcggcaa acttttatga ctgtcttttt ctgtttgtta tcacatgaat
     1501 acggtcaatg aaattatagg cttgctaggt gttgacgcat cttggaagat gagaggtagg
     1561 cagatacttc gtgattttgt tgaatcttat gtgcaatcat cgtaatgcag taaagtgaaa
     1621 tggtggaatt gatgagtatt gaaaaaagag tccctattta tggggctctt tttttatttg
     1681 ttactaatta cttttaaaac gtccacaaaa cgtactacaa aggtctcaaa gagacctaca
     1741 aatagatgtt aaaaggactc aaaacgacct acaagggtgt tttttgaatc ccagttagag
     1801 agctttagaa attcagtagg cacagcctat ttcaccactt tgaactagaa gtgggatcgg
     1861 atttccttat gctctttttt attggaatgg gtctaggaat gaatatagaa atagctgtag
     1921 ttctattcta tggctgttcc gacagtgatt ctcataaaga atgtgattgt atcaatagat
     1981 tggatgatgt ttgaaaagta gagaatagtt gaggctcttc ctgtaaaaca aaaaaataat
     2041 tatgtttgta gaggaagtag tcattccact acaaaaaaat tcccctggat cgtgggaggt
     2101 gtgaaaaaaa tggcgagtgt tggcgaggat attttgcaag gcagcggaga cattatgttg
     2161 tcatggtgaa tataaaaaga gactttttaa tatatggatg ttagcttttg aacggtggag
     2221 gaatacatac agtacctgtc aacggtaagc ggacggaatg aagcgatagc gaaatgatgg
     2281 agcattaagg agttgatagg gaactttgac caagaccctc ttgcagtcct caaaacgcag
     2341 tacgttttgg gctggctacc gccgagggaa tccccttgga gaaggggttg ggggattgat
     2401 atttttatat ggggttgggc aaagcttcag ttaaggagtg ttgtttggat gcacttatag
     2461 tccctttaga cgtaaagcgc agcggaacat aattgtatta agaatatttg ttctccatgc
     2521 tattgtgtga ctctaattca catatagtca tacaaagtaa tattagtttc actttaatgt
     2581 tgtatgaagg tattgcatat tacacattga attttatttg tgtgggatat atgtgaaggg
     2641 gatgaaagca atatgaaggt gtttgaagtg ttagaaagcc tcaagaaagg cgagaaattg
     2701 aaagagatag ctaatgtttt aggaattaac aacagcacgt tacaaagaaa gctgaaggct
     2761 ttggggtatg cttttgatag tagcactaaa gaatggttct acaatgacaa agataaccca
     2821 gagccattag aagaaaatat tatggatatg gtgaagcgaa atagtaaaag tggtgttaat
     2881 gatttaccta agataataca aggtattaaa agcgagacta aaggtgaatt aggagtgaaa
     2941 catggtaata cagtgaaagt atctgaagtt ttgacaagtg aagatatagc agaccttcat
     3001 gaaatgctct tattttggcg tacagataga gaaagaacga aaaagcacaa aactcttcat
     3061 gaagaaataa aaactcttag aagggacgat aggacgcgaa aaacggttat tttgagcaaa
     3121 gaagttgcga aacgtctcga taaactgtca gaaagagagc gttttgacaa gtccgaccta
     3181 ttagaattgg cattggagca ttttttgagt gaatatgaat gaaaatagat tgttgtttgt
     3241 tattaggtcc attatggacc tttttatttt ttttagggaa gattgtagag ctttgtataa
     3301 cgtcctcgca aattattgtt attgatctta cgaggtttgt agagccttgc ataatgtcct
     3361 cgcaaattgt tgatgtgagt atatggggat attatctaaa acttttggag aatggctatt
     3421 ggcatcctaa ggatgccaca gaagaattga atggattatt agcagaagga tagaaagtaa
     3481 taaaaaaaga accctgtaaa taggattctt tttttgtggt tattgacata taaattgtgt
     3541 cttttagaat gtattcaaag ttacttatag atttttaagt cgtaatagaa tgactaagtt
     3601 gtttgacgag aaattttgca tacttttttt cgatttcggc tgttattata ccgttttcta
     3661 aaagtatgta agtgttgttt tgttcgtcag ttagtaatct gatgttatca ttttttgggg
     3721 aaatggcaac atctttttct ttttcattga tattttttaa ttgtatttgc ggtaatgggg
     3781 gtacagtttt gataagtttg ttcatttctt cttgaaattc ttttgtttta actttaccca
     3841 tgtttgtttt aagtaaatta cattttcgta gatctagagg gtttcctgtg atgttgtaac
     3901 atgcttcttt ttgtgtgttg cattctagga gtaatcgaga tagttgtgtt tttgaacttt
     3961 cacctttttt tcttccgatg atattatacc gcattcgttt tccttggcta ttaactaagt
     4021 agttttccca gtaaaaatcg tgagaattag ctatttcgaa gcaatctgta tcaataaaga
     4081 aagcagtttc tttattattg gagtctttga aaaaaattgc cgtatagcca ttttttaagt
     4141 gttcatatct atttttcatg aatttcctcc tctcgttaaa gattatagaa tattcagaac
     4201 tacaataaaa ggttgtaata aaaaaagacg tggtatttga ccacgtcttt ttttatgtgt
     4261 gccaggcatg gcaactatct aggtggtgga agtccactgt gggggtacac atcgaccaac
     4321 cactaaggaa gcgcaaggta cttaccgtga ggtaagggct ggaggaagcg tggaataaaa
     4381 tcttggctcg acgaacagaa acctgatatg aaggctttat aaagggatga ggctccataa
     4441 caagttaaag tccaaaagat gtgcgtaact ttgtagagta aatcaggcga gtaaaagagg
     4501 aaagatagct gccttaccct gggagatctt gcggatgtac taacagtaca gtcgaaaatg
     4561 gttaaccgca agaagtcagc cgaagccata gtagtggaac aaattcatga agggccgaac
     4621 agtttatagt gtttcaacgc catgaatgcg taagtgacgt actccgaata tgttaatgat
     4681 gaaagtatga gcgtatctca aaggataatc aaaatggagt tgtcacttac tacgtgaagg
     4741 gaaaggagaa acgagtgtgg aactcttaga agcaatttta agcaacaaaa atatgaatga
     4801 agcctactta cgcgtttata aaaacaaagg tgttagtgga gttgatggtg taacagtcga
     4861 agaactaaag caatatctga aagagcacaa ggacgagcta cgtcagcgca ttagaacaag
     4921 gaaataccaa ccacaagctg ccttaagagt ggaaatccca aaagagaatg gcaagatgcg
     4981 caagctggga ataccaacag tagtggatag agtcgttcaa caagccattc atcaaatact
     5041 cagcccgata tttgagaagc agttcagtga attcagttat ggttttagac caaaaagaag
     5101 ttgtgagatg gcaattgtta aaagcttgga atttctaaac aacggatatg attggatagt
     5161 agatattgat ttggagagat tctttgacac agttcatcac gataaattga tgcgaattat
     5221 atctaactca atacatgacg gagacgtcat ttctctcata agaaagtatc tagtcagtgg
     5281 agttatggtg aatgggaaat atgaagaaac accagtcgga actccgcaag gaggcaatct
     5341 cagcccttta ctgagtaaca ttatgttgaa cgaactggat aaggaactcg agggtagagg
     5401 acttcaattt gtgagatacg ctgatgacgc tcttatcttt gtgaggagtg agaaggcagc
     5461 gggcagagtg atgaaatcaa tcgtgagatt catagaaaag aaactaggct tgatagtcaa
     5521 tacagaaaag agtaaaatct ctcggccaaa agatttaaaa tttttagggt ttggatatta
     5581 ttacaccctc aaggataaga gatatcaaac gaaaccacat ccaatctcgg tacaaaaatt
     5641 tcaaagaaaa ctgcaccaac tgacaaaacg aagctggagt gtaccgttag actatcgaat
     5701 attgaaactt aaacaagtga tatttggatg ggtcaattac ttcaggagta caaatatgaa
     5761 aggtgttctg gagcgagtag accagaagct ccgcgccaga attagagtga ttatctggaa
     5821 gcaatggaaa gtaccgaaga aacaaatcaa atcgcttgtt caactaggga ttccggagga
     5881 agaagcgaag ggattaacct actgtcgaaa gggcttccga tatatagggt tatcgaaagt
     5941 tgttcaaaga gccatctcaa atcaaagact aaagaagagg ggattccctt cttctttaga
     6001 acgctatcta aaagttcaca ctgtaatata aactgaaccg ccgtatacgg aaccgtatgt
     6061 acggtggtgt gagaggggcg agaataatta ctttattttc cctctactcg attaatttga
     6121 aggtaatttt gatgagtttt cttttatgac atttaaaatt tcgtcaaaag ataatcgctg
     6181 ttctaccacg ttaacagtga agtctgaagc ttcttgttca gatattttta attcgtactc
     6241 attgtataac agaaacattt ctaggcaagc caatgccgtt cttttgtttg cattatagaa
     6301 tgcatggttt ttagccagtg attcaaataa tgcagtggct ttgtggaaga tagtcggata
     6361 tgcatcctct tgtgctactg tttgttgtgg acggtatatt gctgattgta ataaccccgt
     6421 atctttaaca cctctttgtt cgttaggtga atacatatcg ataagtgttt cattgataaa
     6481 aatagcttga gtttctgtta tataaattgt catttcggtt ttcctacttt cctccacgcc
     6541 tgaaggataa ttatctgttt accaaatctt taaatgcacc gtcaaattta ttgatggttc
     6601 tgtttaagga ttccaagaac ttaggattga gaccctctgg catagaaatt tttttggatt
     6661 tttttataac gatttgttcg ttttcttcgt taatttctaa acttacaacg tcaccttgtt
     6721 tgatatttag agtagtgtaa aattcttgcg gtaatgttaa acctaaagaa ttgccgattt
     6781 tagtaacttt acgttcgatt ttttgtgttt ccatgtggcg agtgtttgct gtcatttgga
     6841 cttcctcctt ggaatttgcc acactatttt ttggcgtagt ctaattgcca ctttgtgttt
     6901 gtattgagtt tttcgaatta tatagtgtga ttttatgttt gttttgtttg tttgctttgg
     6961 gttttttcgg tctttttagt tttctctttt gtttgttgtg tttaatctct ctgtattatt
     7021 atgacgttta gttagaaagt tataacagtt agaacgtatt aaccgttaat acaattatac
     7081 tcatttttag aaatatgtaa acaaatttat atttcggtgc aaaagatagg gaagattgta
     7141 gagccttgta taacgtcctc gctacttgtc gctgacaaaa gagggggaag acaaggtgaa
     7201 ttcgtttgga agggcatagc gatacgctat gccctcgcta gtggacgtta gttgtatttg
     7261 tacatgtctc ttttgcgtta aatgttgaat gtaaactttt acacattagt ttacattcaa
     7321 aaatacgaaa tcattgactt tacatcaaaa ttgctgatac tctcaacata tacaacaaaa
     7381 agtatacatt caaaacactg taaataaaga gggtatgggc tgatgagtaa aaaagagcat
     7441 ttgattgatg tacaaccgat tcgaagcaag gagcagttgc aagatatgaa atgggcgctc
     7501 aaacgtcatt gcactgatcg tgattatatt ttattcgtga ttggaatcaa tacgggtctt
     7561 cgtgtgagtg acttattgaa tttagaaaca tctatgattt tggaattgaa gaagaaacgt
     7621 agaaaagaat tgaaaattag ggaaggcaaa acgaaaaagg aacgaatggt gaacattaca
     7681 tctatttttg aagaggttta tttgtatgca gcaggattgg aaagtaaatg gctttttcct
     7741 tctcgcaaag gagacaggcc tataagtaaa atccaagtgt accgacagtt gcaaaaggca
     7801 ggagatttcg caggagtgga atctatcggt actcatacca tgagaaagac tttcggctat
     7861 tggttctata aacaaacaaa agatgttgct atgttgcaag aaattttgaa tcatagcgca
     7921 cctaaaataa cgctcaaata cattggtatc aacaaagagg aaaaagacaa tgtgcttgat
     7981 acgttttgtt tgtaaaaaag agtagaggtg cagagaaagt tacctgttac tctaataagc
     8041 aggtaaaggt tgagaatttc tcacacttct ttaaaaagca tctcggacaa gcacacaaaa
     8101 atatctgttt tggggttaag aatttgctat gaatgatgac gtcacaaccc ccctatgtaa
     8161 tacgggttgt gtgaccccaa aaataaaggg gttaaatcta tcgaaaatac aggaacatca
     8221 agggttttca ggtcttttat ttgtgtaaaa aaagtgtttt aaaaaaatga gaaaaaaata
     8281 cactttaaaa tttatggaga aaaccatgaa agaaaggata ctaattgacg agtttatttc
     8341 tttgttacgc tatcatcatg tattccttaa ggggaaaata ttataaatct cttaaaaacg
     8401 tttagaagtg aaaatgaagc gtttgggaga tagaagaatt tttgtgtttg aaagaaaaaa
     8461 atgcccttgt ccacgaagtg gattgagggg cattttttta tgatttttcg gacacgaaaa
     8521 atacggtttt cttctttctt taatacatag gtaactattt cggcaacgaa attgatgcct
     8581 atgtatggtt ttatcccttg atacacaagg gatttctaaa aaaaaagact tgcattccta
     8641 ggtggataga tttatcattt ttagtgtcga attaaaaaca ataactacca agaaaggagc
     8701 aagtccgaaa aagggattcg tatacccttt tttaagcaat tcagatagga ctaatatatc
     8761 aaacatgaca aagaaaaatc aagatgaaat cactggtaaa ttacaaccta aaaagaaaca
     8821 gaatataaga atttatgaat ttatagagaa gaaaatgagt gagagcggga gagaattgtt
     8881 taaatcatgt agtactttta atgagtttgt ggctacgaaa gataaaaaga aaaagaaacg
     8941 agtaaagggg aatgattgca aaaatcgttt ttgtccaatt tgcgcttgga gaaaagcagg
     9001 aaaggacgca gtgaaaatcg caacgatgat ggaagcaatc aagattgaag aaaaaaaaga
     9061 gtttcttttc ctaacattaa caaccccaaa tattaaagct gatatggtaa aaggcgagat
     9121 tgatagattt aataaagctt ttaaaaagtt atttgataga cgtaatattc aacgctctat
     9181 taagggttat attagaaagc tagaaatgac gtatgataaa gaacgattta tcactgaaga
     9241 aatgtataac aataaaaaga gaaaagcata ttatgattct cgtggcttga aagtagatga
     9301 tcataacccg aattatgata catataatcc tcattttcac gtattacttt gtgttgataa
     9361 aagctatttt aagaaaaaag aactttatat taaacaggaa gaatggttag aaatgtggcg
     9421 agaagtaaca gatatgccag agattacgca agtacatatt caaaaagtag agttaatcag
     9481 agaaggaaat gcagttgcgg aggttgcgaa gtattcagca aaagattatg aaatgtcagt
     9541 gagtcaagat gtgtttgacg tgttttattt agctttaaaa ggtcgacaat taattgtgta
     9601 cgctggattg ttaaaagatt atgcgaaaaa atacgaaaat ggcgaattgg acaagtataa
     9661 gagtatagat aaaaatgagt attattatag attaattgca atgtggaata aggatttgat
     9721 gaagtttgaa caagaatatc aagagttaac ggaaagtgag aaacaagaat ataacggtca
     9781 tttgattgat gaaatggagg ttgaatagtt ttgtataaaa aaggtgatat tgttaaacat
     9841 acctgttggg aatgttataa cgaagaaatg aaaatttatc ggatttcaaa aagtaaggtc
     9901 aaagatggtg aagatgtata ttactcatat aaatgttggg ttaaatgtga aaaatgtggt
     9961 gcggttgatg atgtttgaat gacaaagaga gaaccaggct taatattaag tttggttctc
    10021 tctttgttgt attgaaagaa aaccttctta gtgtagcaag tagcattcaa aagttttttt
    10081 atgatctaac tgagaactaa caagaaagat tttttcttaa atttaaattt cgtgaatttg
    10141 tgagtttatt tttaaagttt atttcgagat atattttgtt ctgatgattt cgttttacta
    10201 ctgtacgagg tgaaaaccta aggtgtgcgc atgaattttt ttagatatat ttatatcgat
    10261 aaaataccgc acgtttttta atattttttt gaaaataagt tttgggaaat tctaaatttt
    10321 ttttgaggtt gattttggac ttttgatatg agatttgatg ttgaaattta ttaaatttga
    10381 aatctcgatt tttcgatttt cgaaattttt gttttccgtt ttttctgtaa ttctgtactc
    10441 caaaattacg gagtttatat agtacctcca tgacgaactg aaaacgaaaa aactttgctc
    10501 gcatattcaa tctatagata ctttgtatct ttaaaaagtt gcaccgtttt aaatgagtta
    10561 ttatagatcg tgtgatgaaa aaaagatgtt tggattatat ttgaatttgt tatttccgtg
    10621 aattttttat ttcgtgattc ctgtatattt ttaatcc
//
