LOCUS       NZ_CP083128             2062 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain SA11 plasmid pCH_164-l, complete
            sequence.
ACCESSION   NZ_CP083128
VERSION     NZ_CP083128.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214337
            Assembly: GCF_020809145.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x, 70x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809145.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 13:17:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,862
            CDSs (total)                      :: 6,710
            Genes (coding)                    :: 6,385
            CDSs (with protein)               :: 6,385
            Genes (RNA)                       :: 152
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 104 of 325
            Pseudo Genes (incomplete)         :: 245 of 325
            Pseudo Genes (internal stop)      :: 76 of 325
            Pseudo Genes (multiple problems)  :: 76 of 325
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083128.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="SA11"
                     /isolation_source="Biopesticide Delfin"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_164-l"
                     /collection_date="2017"
     gene            complement(join(1936..2062,1..743))
                     /locus_tag="K8Z41_RS34125"
                     /old_locus_tag="K8Z41_34050"
     CDS             complement(join(1936..2062,1..743))
                     /locus_tag="K8Z41_RS34125"
                     /old_locus_tag="K8Z41_34050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198524.1"
                     /translation="MQVYLDRLMIKYKDVTEKQFSDVLTKISSKQIFLPNTPIRSEHGT
                     SVRDYHRVIHIGYGEGAVYIGWKHNSEKEKDSYDMKVDFNPSKFENNELQKDSYEKVFE
                     TVFHTLNAVLKSNKRVVYGMDIAFDIERHMSDIVSYSKTGKQQDRHKGTVYYGNRNKDG
                     YLKIYDKKKELYNHFKRMIEEENLTRIEYSWRDSDGVVVDEIRKSPPFSIDESYTFSIF
                     NLNNVKGALKACLICYSNGTMDMKEFPRRTKESIKKALEEMDHLAVDPILQDCWLSILE
                     NIKNYTRL"
                     /name="hypothetical protein"
     gene            complement(844..1071)
                     /locus_tag="K8Z41_RS34130"
                     /old_locus_tag="K8Z41_34055"
     CDS             complement(844..1071)
                     /locus_tag="K8Z41_RS34130"
                     /old_locus_tag="K8Z41_34055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002100596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000572905.1"
                     /translation="MIESEGNNLKLTERQLNDLKRISELRVKLFGVPGESVVDPENVEF
                     LLDNAISSYLGQLEIFEVTIEIEQYNSMCG"
                     /name="hypothetical protein"
     gene            complement(1506..1748)
                     /locus_tag="K8Z41_RS34135"
                     /old_locus_tag="K8Z41_34060"
     CDS             complement(1506..1748)
                     /locus_tag="K8Z41_RS34135"
                     /old_locus_tag="K8Z41_34060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001293418.1"
                     /translation="MSEMVRVNTRISKKLNDWLDEYSKESGVPKSTLVHLALENYVNQK
                     VMLEQMPKMQQMLSMMFENVTQQQLNQKGNMFELK"
                     /name="hypothetical protein"
     Typing          complement(2..743)
                     /name="rep_cluster_1434"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aactctttca tatccatagt tccattagaa taacaaatca aacaagcttt taatgcccct
       61 ttaacattat tcaaattaaa aatcgagaat gtataagatt catcaatact aaacggagga
      121 ctcttcctta tttcgtctac cactacaccg tcagagtctc tccaactata ctcaatacga
      181 gtcaaattct cttcttctat cattctttta aaatgattat ataactcctt tttcttatca
      241 tatatcttca aatatccatc tttatttcta tttccataat aaacagttcc tttatgtcta
      301 tcctgttgct ttcctgtttt actataagac acaatatcac tcatatgacg ctctatatca
      361 aaagcaatat ccataccata aaccactcgc ttattagact tcaaaactgc atttaacgta
      421 tgaaaaacgg tttcaaacac tttttcataa ctatcttttt gcaactcgtt attttcaaat
      481 ttagaagggt taaaatcaac tttcatatca tagctatctt tttccttttc cgaattatgt
      541 ttccacccta tataaactgc accttcacca tatccaatat gtataactct atgataatct
      601 ctaacagacg tcccatgttc tgacctaata ggtgtattcg gtaaaaaaat ctgctttgac
      661 gatattttag ttaaaacatc actaaattgt ttctctgtta catctttata cttaatcatt
      721 agcctatcca aataaacttg cataaaatct atcccgctcc ttatttttta ttaaacatgt
      781 cattttgtac acgattttcg gctattagaa tgtccgaaaa tgtcttcgcc acatttctac
      841 aatttaccca cacattgaat tatactgttc aatctctatc gtgacttcaa aaatttctaa
      901 ttgccctaaa taagaactaa tagcattatc caataaaaac tcaacattct ctggatcaac
      961 tacactttca ccaggaactc caaacaactt tacacgtaat tcgctaattc ttttcaaatc
     1021 attcaattgt ctttctgtca gtttcaaatt gttaccctca ctttctatca atctgctcct
     1081 acacctccgt tacctcgccg accgtacgct cttctcactc attccatttc gtttgccatc
     1141 agttcacgcg ccgtcgtgtc ctttgagagt ccactccgtc accttacctt ctgtctgcac
     1201 ttcacttcat tcatgtcgct cccttaagtc gtctacttcc ggctccgtcg aatacactta
     1261 ataaataagt gcccatttac acagttaact gccatgggtt tcctttcagt catacaacgt
     1321 tcacccaagt gaataataat tacaactcat acgattacaa aagccgtttc ctaattgttc
     1381 agggacccat gcacggatat tctaaatgag taggtggcgg gcaaacttca gccacaccaa
     1441 ctacgcattt agtttatcga tgtgcatctc ctaatcaata agactacatt ttcgaaacat
     1501 aaccgttact tcaactcaaa catattccct ttttgattca attgttgctg cgttacattt
     1561 tcaaacatca tactcaacat ttgttgcatc tttggcattt gttccaacat aaccttttga
     1621 ttcacataat tctctaaagc taaatgaact aaagtgcttt tcggtacacc actttctttg
     1681 ctatactcgt ccaaccaatc atttaacttt ttactgatac gtgtattaac acgaaccatt
     1741 tcactcatct aatcacctct tgtatatcgt actacataaa tataacatca gtttaaacac
     1801 aatatcaaca ctaagataca ccagaacaat gtaaaagtgg gactctatcc gctattaata
     1861 taaacaagtt aagaaaaaca aaaagagagc atcatattga caccctcttg acacacagag
     1921 aagcacacgc taatatcata aacgagtata gttcttaata ttttctaata tagataacca
     1981 acagtcctgt agaatggggt ccaccgccaa gtgatccatt tcttcaaggg ctttttttat
     2041 actctcttta gttctacgag gg
//
