LOCUS       NZ_CP083127             5120 bp    DNA     linear   CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain SA11 plasmid pCH_164-k.
ACCESSION   NZ_CP083127
VERSION     NZ_CP083127.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214337
            Assembly: GCF_020809145.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x, 70x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809145.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 13:17:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,862
            CDSs (total)                      :: 6,710
            Genes (coding)                    :: 6,385
            CDSs (with protein)               :: 6,385
            Genes (RNA)                       :: 152
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 104 of 325
            Pseudo Genes (incomplete)         :: 245 of 325
            Pseudo Genes (internal stop)      :: 76 of 325
            Pseudo Genes (multiple problems)  :: 76 of 325
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083127.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="SA11"
                     /isolation_source="Biopesticide Delfin"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_164-k"
                     /collection_date="2017"
     gene            324..500
                     /locus_tag="K8Z41_RS34085"
                     /old_locus_tag="K8Z41_34010"
     CDS             324..500
                     /locus_tag="K8Z41_RS34085"
                     /old_locus_tag="K8Z41_34010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976754.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_015976754.1"
                     /translation="MSKLLTAQEVADLLRVHKKTIHRMIHSGKLDASKVANKFLIKEED
                     AKGLLENKNKMDN"
                     /name="helix-turn-helix domain-containing protein"
     gene            516..1016
                     /locus_tag="K8Z41_RS34090"
                     /old_locus_tag="K8Z41_34015"
     CDS             516..1016
                     /locus_tag="K8Z41_RS34090"
                     /old_locus_tag="K8Z41_34015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976755.1"
                     /translation="MENNTKQLNNEVNEIAVVDEHVNTNDIVTVDKVSPSIFEADGAKD
                     VFFSSIQSKDRKSAIKVYNAINSSENALADHRGEVLEITDMVAHAITLQDELTKEDVDA
                     LRVVLIDKNGKAYHAVSQGVVSSIQKIISIVGPAPWNEPLEIVPAEVKTRKGFKTLTLQ
                     LQG"
                     /name="hypothetical protein"
     gene            1088..1312
                     /locus_tag="K8Z41_RS34095"
                     /old_locus_tag="K8Z41_34020"
     CDS             1088..1312
                     /locus_tag="K8Z41_RS34095"
                     /old_locus_tag="K8Z41_34020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976756.1"
                     /translation="MNAIKNLVQVSIRTNERELEFVSFNGMVHLKQKLEEDETSVVLTK
                     EELDMVAAMSNFDNVIANFMNSITEKKAM"
                     /name="hypothetical protein"
     gene            1371..2108
                     /locus_tag="K8Z41_RS34100"
                     /old_locus_tag="K8Z41_34025"
     CDS             1371..2108
                     /locus_tag="K8Z41_RS34100"
                     /old_locus_tag="K8Z41_34025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016090431.1"
                     /translation="MANKRLKKKLETKRKKSLLVSEGYSKKETKKLKGRELETVYKKKA
                     HNRKNRERAREIANLARQWGLSPSKFNSWKKLLPEIERIKKEQDREAPFLVIYYQDFTG
                     ETDSKFIYDFKKRNNTRSRSQITRSIIGWLQNAQNKLFLGRVAMRIVPKRDVSKTNTLW
                     KNHGYVKIYEGQGKELTKLLTAIETIMVGVYDVKDRDKYLKQLLNNLRSLPYKQAHRNA
                     NEIQKIYDTKSYTKESWDNDEYY"
                     /name="hypothetical protein"
     gene            2095..4299
                     /locus_tag="K8Z41_RS34105"
                     /old_locus_tag="K8Z41_34030"
     CDS             2095..4299
                     /locus_tag="K8Z41_RS34105"
                     /old_locus_tag="K8Z41_34030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090430.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO:0000166 - nucleotide binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="WP_016090430.1"
                     /translation="MSTTNRKKRREIKLFTLDTETRGLDGDVFRIGLFDGKQYYTGYTF
                     ADVLPVFEKYKAYDCHVYIHNLDFDLSKIIAELRDYAEPTFNNSLFINGNIVTFTASHI
                     ILHDSFRLLPSSLENLCRDFDLLEGGKMDIVDYMEENNYGIYNVKNRKLNKRLTKGNFF
                     TTVDKDDPVLCEYMEYDCRSLYKILEIVIGLSKLEVEQFINCPTTASLAKTVYKEQYKK
                     DYKVAISTKQYNHKQLGKGLEAFIRKGYYGGRTEVFTPRIENGYHYDKNSLYPYVMKMA
                     EMPVGYPNVLDNEEAELSFDLWKRRRYGAGFIHAKVHVPEDMYIPILPKKDYTGKLIFP
                     VGKIEGVWTFPELALAEAEGCKIEKIESGVVFEKTAPVFREFISYFEEIKNTSKGAKRA
                     FSKLMQNALYGKFAMQRERIMYADISERDKLEAEGHTVSEIIYDMNGIRMEFLEYDGYA
                     MAEYIQPHISAYITSIARILLFKGLKYAHEKGILAYCDTDSCATTTKFPDKMVHDKEYG
                     KWKLEGYVIEGLYFQPKMYAEKAINTDGEYEEVLRMKGVPKWVVEEQLDYNSFRKWYLQ
                     VKRGKAEIPIYKGGERVQKFLTKSKNNIEMNELAEMHKTINFAREQKRNIDLNKNITSP
                     LVRNDYGENKDEKSEYEFDEWYERLEEFNDDMNAVEELCMKFGKIQIPEKKQRKLYGLY
                     KEYSSKAKAMCFSNEGLPIQDWCKKTGWDMKELLGELSFL"
                     /name="DNA polymerase"
     gene            4316..4516
                     /locus_tag="K8Z41_RS34110"
                     /old_locus_tag="K8Z41_34035"
     CDS             4316..4516
                     /locus_tag="K8Z41_RS34110"
                     /old_locus_tag="K8Z41_34035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957403.1"
                     /GO_component="GO:0009289 - pilus [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="WP_000957403.1"
                     /translation="MLTPREQDTLECISGYMEEFGFAPSVRDMASRMYVSHKTAHRYLM
                     QLESKGRIRRVHQRPRAIQLL"
                     /name="FaeA/PapI family transcriptional regulator"
     gene            4530..4682
                     /locus_tag="K8Z41_RS34115"
                     /old_locus_tag="K8Z41_34040"
     CDS             4530..4682
                     /locus_tag="K8Z41_RS34115"
                     /old_locus_tag="K8Z41_34040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016090429.1"
                     /translation="MRDKLLDFIIELSQSSKQVVSKSYVIDRLMQVTKEDYKELEKKTE
                     NAEKD"
                     /name="hypothetical protein"
     gene            4800..5054
                     /locus_tag="K8Z41_RS34120"
                     /old_locus_tag="K8Z41_34045"
     CDS             4800..5054
                     /locus_tag="K8Z41_RS34120"
                     /old_locus_tag="K8Z41_34045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976760.1"
                     /translation="MDCKTLVKRYEEQNEELRKLNSILTKRIYHLLQENESLTSHVSFM
                     RDFNEELIRDNMNYLGDKLDEKLQDIEGDDSNDRHAKSN"
                     /name="hypothetical protein"
ORIGIN
        1 ttacctattg acacgtaata acatttatga aatatgatat acaaaatttt ctaaaattaa
       61 ctattgacaa tttggaaaat ttattggaat ttccaaaagg aaaagtgtga caagatattc
      121 caagggtgct ataataggaa atgagttgaa aacgaacacg cgttttatag atgttcggtt
      181 tttgtaacat aactgtaaca taaaaacatt tgcattactg gtaactttcg ggtaatattg
      241 gtagcagaga gggacaaacg ggacaaatcg agacacgata catactctct atacaagtga
      301 aaagaaggtg gtgcatatgt aatatgagta aactactgac tgcgcaagag gttgcagact
      361 tattgcgtgt tcataaaaag actatccata gaatgataca ttctggaaag ttagacgctt
      421 ctaaggtagc aaacaaattc cttattaaag aggaagatgc aaaaggacta ttggaaaata
      481 aaaataaaat ggacaattaa taggaggaat attcaatgga aaacaatact aaacaattaa
      541 ataatgaggt taacgaaatc gcggtagtgg acgagcatgt aaatacaaat gacatcgtaa
      601 cagtggacaa ggtgtcacct tcaatctttg aagcggacgg agcgaaggat gtattcttct
      661 catctattca aagtaaagat cgtaaatcag ctatcaaagt ttataatgct attaactcta
      721 gtgagaatgc tctagcagat catagaggtg aagtattaga aatcacggac atggttgctc
      781 atgctattac tctacaagat gaattgacaa aagaagatgt ggatgcatta cgtgttgttt
      841 taattgataa gaacggtaaa gcataccacg cggtgtcaca aggggtcgta tcatctattc
      901 aaaagattat tagtattgta ggtccagcac cttggaacga gcctttagaa attgttccgg
      961 cagaagtgaa aacacgtaaa ggatttaaaa cattaactct tcaactacaa ggctaatttt
     1021 tgtaacaaat gaatgagggt gcacattgtg tatcctcttt tttaaaacat aaaaggatgg
     1081 tgcggtcgtg aacgctatta aaaacttagt tcaagtatct attcggacaa atgaacgaga
     1141 actggaattt gtctctttta atggaatggt gcatttgaag caaaagttag aagaagatga
     1201 aacatccgtc gtgttaacaa aagaagagtt agacatggtt gcagctatgt ctaattttga
     1261 taatgtgata gccaacttta tgaactctat tacagaaaag aaagccatgt aagccaaaga
     1321 cctgtgacct agttgttacg ggtcacatgg ctttataagt aggtgataca atggcaaata
     1381 aacggttaaa gaagaaatta gaaacgaaac gtaaaaagtc gttacttgtt tcagaaggtt
     1441 atagtaagaa agaaacaaag aagctgaaag gtagagagtt agaaactgtt tataaaaaga
     1501 aagcgcataa taggaaaaat agagagcgtg ctagggaaat agcaaatcta gcaagacagt
     1561 ggggactttc accgagtaag tttaattcat ggaaaaagtt acttccggaa atagagagaa
     1621 taaaaaaaga acaagatagg gaagcacctt ttctagtaat atactatcag gactttacag
     1681 gtgagacaga cagtaagttt atatatgatt ttaaaaagag aaacaataca agatcacgta
     1741 gccaaattac taggtctata ataggttggt tgcagaacgc ccagaacaaa ctatttttag
     1801 gaagggttgc tatgcggatt gtcccaaaac gtgatgtatc taaaaccaat acactttgga
     1861 agaatcacgg ctatgtaaag atatatgagg gacaaggaaa agaacttaca aaactattaa
     1921 cagcaataga aacgataatg gttggtgttt acgacgtaaa agaccgtgac aagtatttaa
     1981 agcagttatt aaataatctt cgttccttac catataaaca agcacatagg aacgctaatg
     2041 agattcagaa gatatatgat actaaatcat atacgaaaga aagttgggat aacgatgagt
     2101 actactaata gaaaaaagcg tagagagatt aaactcttta cgctagatac tgaaacacgt
     2161 ggtttggacg gcgacgtgtt tcgaattgga ctattcgatg gaaaacaata ctatactggc
     2221 tatacgtttg cagacgtatt accagtgttt gaaaagtaca aagcatatga ctgtcatgta
     2281 tatatacata acctagactt cgacctttcc aagatcatag cagagttacg tgattatgct
     2341 gaaccaacat ttaataactc gctatttatc aatggtaata tagtaacgtt taccgcttct
     2401 catattatac tacatgatag cttccgattg ctaccgtctt cacttgaaaa cttatgtaga
     2461 gatttcgacc ttttagaagg tgggaagatg gatattgtcg attatatgga agaaaataat
     2521 tacggtattt ataatgtaaa aaaccgtaaa ttgaacaagc gacttacaaa aggtaatttc
     2581 tttacaacag tagataaaga tgaccctgtc ctatgtgagt acatggaata tgattgtcgt
     2641 agtttatata aaatcttgga aattgtaata ggcctatcaa aattagaagt tgaacaattt
     2701 attaattgtc cgactacagc ttccctagct aaaacagttt ataaagaaca gtataagaag
     2761 gattataaag ttgctatttc tacaaaacaa tataatcata aacaactagg aaaaggttta
     2821 gaggcattta ttagaaaagg ctactatgga ggaagaaccg aagtttttac accaagaata
     2881 gaaaacggat accactatga taaaaatagt ttatatccat atgttatgaa aatggctgaa
     2941 atgccagtcg gatacccgaa tgtactggat aatgaagaag cagaactttc ttttgatcta
     3001 tggaaaagaa gaagatatgg agcaggcttt atacatgcga aggtacatgt acccgaagat
     3061 atgtatattc ctatcttacc taaaaaagac tatacaggta aattgatttt cccagttggt
     3121 aaaatagaag gtgtttggac atttccggaa ctagctttag cagaagcgga aggttgcaaa
     3181 atagaaaaaa tcgaaagcgg tgttgtattt gagaaaacag cacctgtctt tcgtgaattt
     3241 atttcgtatt ttgaggaaat aaaaaataca agtaaaggtg ctaaacgagc cttttcaaaa
     3301 cttatgcaga atgcgttgta tggaaaattt gcaatgcagc gggaacgaat catgtatgct
     3361 gatatttctg agagagataa acttgaagca gaaggacata cagtaagcga gatcatatat
     3421 gatatgaatg gaattcgaat ggaattttta gagtatgacg gatatgctat ggctgaatat
     3481 attcaaccac atatttcagc atatattact tctattgcaa gaattctact atttaaagga
     3541 ttgaagtatg cgcatgaaaa aggaattttg gcttattgtg atacggacag ttgtgcaaca
     3601 actacgaaat ttcctgataa aatggttcac gataaagagt atggaaagtg gaagttagaa
     3661 gggtatgtta ttgaaggact ctatttccaa ccaaaaatgt atgcggaaaa agctattaat
     3721 accgatggtg aatatgagga agtattacga atgaaaggtg ttccgaaatg ggttgtagag
     3781 gaacaactag actacaattc atttcgtaaa tggtatttac aagtaaaacg tgggaaagcc
     3841 gaaattccta tttataaagg cggggaacgt gttcaaaaat tcttgacgaa atctaaaaat
     3901 aatatagaaa tgaacgaact agcggaaatg cataaaacaa ttaattttgc tagggaacaa
     3961 aaaagaaaca ttgatctaaa taaaaatatt acctcacctc tagttcgtaa tgactatggg
     4021 gaaaataagg acgaaaagtc agagtatgag tttgacgaat ggtatgaaag attagaagag
     4081 tttaacgatg atatgaatgc ggtagaggaa ctttgtatga agtttggaaa gatacaaata
     4141 ccagagaaaa agcaaaggaa gttatacgga ctttataaag agtattcttc aaaagcgaaa
     4201 gctatgtgtt tttcaaatga gggacttccg attcaagatt ggtgtaaaaa gacaggatgg
     4261 gacatgaaag aactattagg cgaattaagt ttcttataat agggagtgac gaaaaatgtt
     4321 gacgccaagg gaacaggata cgttagaatg tataagcggg tatatggaag agtttgggtt
     4381 tgcaccgagt gtgcgagata tggcgagtcg aatgtacgta agtcataaga cagcacatcg
     4441 ctatttaatg caattggaaa gtaaaggacg aattaggaga gtgcatcaaa gacctagagc
     4501 catccagttg ttataaggag gggaaaggta tgcgtgacaa attgctcgac tttatcatcg
     4561 aactatcaca atctagcaaa caagtcgtaa gcaagtcgta tgtcatcgac aggttaatgc
     4621 aagtaacaaa agaagactac aaggaactag aaaagaaaac agaaaatgcg gaaaaggact
     4681 agcaaagttg ctagtctttt ttatttgtcc aaaatgacac acgtgtgacg ttatgcggaa
     4741 caattgttcg tattatagta tacatataca gaacaaacat tcgataagga gtgtgacaga
     4801 tggattgtaa gacgttagta aagcgatatg aagaacaaaa tgaagaacta cgaaaattaa
     4861 attctattct tacaaaacga atctatcatt tgctacaaga aaatgaatca ttaacaagtc
     4921 atgtatcgtt tatgagagat ttcaatgaag aattaatacg cgacaacatg aattatttag
     4981 gcgataaact agatgaaaaa ttacaagata tagaaggaga tgattcaaat gacagacatg
     5041 ctaaaagcaa ttgaagaaaa cccctttcta ttacgattcg gtgacggaca attcttcgcg
     5101 cgtaagaaaa acgaggaaga
//
