LOCUS       NZ_CP083126             7635 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain SA11 plasmid pCH_164-j, complete
            sequence.
ACCESSION   NZ_CP083126
VERSION     NZ_CP083126.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214337
            Assembly: GCF_020809145.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x, 70x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809145.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 13:17:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,862
            CDSs (total)                      :: 6,710
            Genes (coding)                    :: 6,385
            CDSs (with protein)               :: 6,385
            Genes (RNA)                       :: 152
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 104 of 325
            Pseudo Genes (incomplete)         :: 245 of 325
            Pseudo Genes (internal stop)      :: 76 of 325
            Pseudo Genes (multiple problems)  :: 76 of 325
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083126.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="SA11"
                     /isolation_source="Biopesticide Delfin"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_164-j"
                     /collection_date="2017"
     gene            407..607
                     /locus_tag="K8Z41_RS34040"
                     /old_locus_tag="K8Z41_33965"
     CDS             407..607
                     /locus_tag="K8Z41_RS34040"
                     /old_locus_tag="K8Z41_33965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139252680.1"
                     /translation="MNIRISVVLPYGYSYSDSNRKCDCINVFGVFERIEKAEALPVKQK
                     NMFVEEVVISLQENSHRSVGR"
                     /name="hypothetical protein"
     gene            1018..2223
                     /gene="mobV"
                     /locus_tag="K8Z41_RS34045"
                     /old_locus_tag="K8Z41_33970"
     CDS             1018..2223
                     /gene="mobV"
                     /locus_tag="K8Z41_RS34045"
                     /old_locus_tag="K8Z41_33970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033078.1"
                     /translation="MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNY
                     DLIHDKRKVDYKKFIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVSLQQEIEKLEQEKKEIDSRLYDLAS
                     SLDQAKSVDEIPVKEKGGFIRSKMVEIASEDFDSIKSLAKSSESLRNENRRLKNEKIKI
                     EREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGVIGY
                     VKGSILDKMNRKFLKRHFAGDDEVKGAQKFLNHKQEHEEQQKRLKQVRRSQQKNWDQGL
                     ER"
                     /name="mobV"
     gene            2255..2536
                     /locus_tag="K8Z41_RS34050"
                     /old_locus_tag="K8Z41_33975"
     CDS             2255..2536
                     /locus_tag="K8Z41_RS34050"
                     /old_locus_tag="K8Z41_33975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003273526.1"
                     /translation="MEYFHSYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNDQIMGLSGGLRAISERLRDMNENVVLVSLEDKE"
                     /name="hypothetical protein"
     gene            complement(2698..3099)
                     /locus_tag="K8Z41_RS34055"
                     /old_locus_tag="K8Z41_33980"
     CDS             complement(2698..3099)
                     /locus_tag="K8Z41_RS34055"
                     /old_locus_tag="K8Z41_33980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000158319.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKMSEQESSD
                     FTVNVVEQRLSFEEILKIIKENSSKLPLN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(3137..3421)
                     /locus_tag="K8Z41_RS34060"
                     /old_locus_tag="K8Z41_33985"
     CDS             complement(3137..3421)
                     /locus_tag="K8Z41_RS34060"
                     /old_locus_tag="K8Z41_33985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_000126403.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEDLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            4292..5335
                     /locus_tag="K8Z41_RS34065"
                     /old_locus_tag="K8Z41_33990"
     CDS             4292..5335
                     /locus_tag="K8Z41_RS34065"
                     /old_locus_tag="K8Z41_33990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_000162904.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIRIYEFIEKKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADTVKSEIDRFNKAFNKLFKRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVEKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGHL
                     IDEMEVE"
                     /name="protein rep"
     gene            complement(6340..6603)
                     /locus_tag="K8Z41_RS34070"
                     /old_locus_tag="K8Z41_33995"
     CDS             complement(6340..6603)
                     /locus_tag="K8Z41_RS34070"
                     /old_locus_tag="K8Z41_33995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874966.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(6617..6904)
                     /locus_tag="K8Z41_RS34075"
                     /old_locus_tag="K8Z41_34000"
     CDS             complement(6617..6904)
                     /locus_tag="K8Z41_RS34075"
                     /old_locus_tag="K8Z41_34000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000323412.1"
                     /translation="MAYIISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            7262..7489
                     /locus_tag="K8Z41_RS34080"
                     /old_locus_tag="K8Z41_34005"
     CDS             7262..7489
                     /locus_tag="K8Z41_RS34080"
                     /old_locus_tag="K8Z41_34005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          4158..5335
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1019..2220
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttctttctt tttctgtttg ttatcacatg aatatggtaa atgaaattat aggcttgcta
       61 ggtgcgtcaa catcttggaa gatgagaggt aggcagatat ttcgtgattt tgtttaactt
      121 tatgtgtgat tatcgtaatg cagtaaaatg aaatggtgga attgatgagt aatgaaaaaa
      181 gagttcctat ttgtggggct ctttttttat ttgtcatggt gtatgcaaag cacatgcaga
      241 atacatacta aacgttttta aacgtataga ggacgcatac tttagggaaa atcgcttccc
      301 tgaagtagag catgagaaac ccaataggca cagtctattt caccactttt tgtacaaagt
      361 gggattcggt ttccttatgc tcttttttat ttgaatgggt ctaggaatga atatacgaat
      421 atctgtagtt ttaccctatg gctattctta tagtgattct aatagaaaat gtgattgtat
      481 taatgttttt ggggtgtttg aaagaataga aaaagctgaa gctcttcctg taaaacaaaa
      541 aaatatgttt gtagaggaag tagttatttc actacaagaa aattcccata gatcggtggg
      601 gcggtgaata taaaaagaga ctttttaata tatggatgtc agattttaaa agggggagga
      661 gtatttatgg tacctgtcaa cggtaagcgg acggaatgaa gcgatagcga aatgatggag
      721 cattaaggag ttgataggta acttcgacca agaccctctt gttcttccca aaaccatgtt
      781 ttggggatgc ctaccggcga gggagtccct caaggattga ggggttgggg agttcgattg
      841 aaaggggtca aggggaagag tttcccttgt gggtttgggc aaagcccata attcttttgc
      901 catgcctgtc atggctcggc ggtcgccgaa atgtcccgta gggcacacgt gtttacgcag
      961 taaactgtag tgtgctacag tttacatgga actacgaccg aagaaggagc gatgcccatg
     1021 tcaaaattca tggtttgtca tatgcaaaaa ttcaagatga cagatgtgaa aggattacaa
     1081 atacataatc agcgtgaaaa agaaagtcat tcaaattcag acattattca agaacgaacg
     1141 gaacagaatt atgatcttat tcatgataaa agaaaagtgg attataagaa attcattcaa
     1201 aatcgaattg ataatggtgt tgtatctaat cgtgcgattc ggaaagatgc tgttgtttgt
     1261 tgttcgttta tgatttcggc tagtcctgat tatatgaatt ctttatctca agcggaacag
     1321 agacgattct ttgaagagtc tgtttgtttt ttaaaggcga gatatggaga agagaacttt
     1381 gtatatgctt cagtacatgt tgatgaaaaa acacctcata tgcatgtggg catggttccg
     1441 gttaatgaaa aacaaaaact atcagcgtat tcatttttta aaaacaaatc agagcttcat
     1501 gatctacaag ataaaatcta tgaacatgtg aaggaaaagg gatttgatat agagcgtggt
     1561 gtttctagcg acaggaaaca tttatcgacg cagcggttta aagcggtgag tttgcaacaa
     1621 gaaatagaga agttagagca ggaaaagaaa gaaatagata gtcgtcttta tgatttagca
     1681 tcgtctttag atcaagcgaa aagtgtagat gagattccag tcaaagaaaa aggcgggttc
     1741 atacgctcta aaatggtcga gattgcttca gaagacttcg atagtataaa atcattagct
     1801 aagtcttctg agtctcttag aaacgaaaat agacgcctta aaaatgaaaa gattaagatt
     1861 gaacgtgaaa aagatgattt atataagggg caacgttttt tagaaaggca agtaacagac
     1921 ttgaaaagag agaatcgagg gttgaaggaa gcaaatgatt ttcttaagaa aacgttggag
     1981 cgagtgaaag agatgtataa ggagaagctt cctgaactcg caggggtgat tggttatgtt
     2041 aaggggagta tactggacaa gatgaatcgc aagtttttaa aacgacattt tgctggtgat
     2101 gatgaagtaa agggtgctca aaagttcttg aatcacaaac aagagcatga agaacaacaa
     2161 aagagattaa aacaagtacg aagaagccaa cagaaaaatt gggaccaggg attggagaga
     2221 tagtgtggga acattattta atcaacaacc aaggatggaa tattttcata gttatggagt
     2281 taagtttata ggtactgtaa aggaattggc taagaaaaat aatttaacga ttgaagagac
     2341 atgtaggatt ttggaattga cgatgaaaat tgatgattat gatcgtaagg atgaacaatt
     2401 agctggaatt ggagaactta tcaaggaaat gaatgatcaa attatgggac ttagtggagg
     2461 tctgagagct ataagtgaac gtttgcgtga tatgaatgaa aatgttgtat tagtatcttt
     2521 ggaagataaa gaataatggc atgttaggag gctctgtgtg agtctctttt ttctttagat
     2581 gtgtagcgtt agcggaactt gttttgcata atatagcgag gattgtagag ccttgtataa
     2641 catccttgaa gatagtttgt ttaataaaaa agacgtggtt aaatcccacg tctttttcta
     2701 atttaaaggt aattttgatg agttttcttt tataattttt aagatttctt caaaagataa
     2761 tctctgttct actacattaa cagtgaaatc tgaagattct tgttcggaca tttttaattc
     2821 gtactcattg tataacagaa acatttctag gcaagctaat gccgttcttt tgtttgcatt
     2881 atagaatgcg tggtttttag ccaatgattc aaataatgca gtggctttgt ggaagatagt
     2941 cggatatgca tcctcttgtg ctactgtttg ttgtggacgg tatattgctg attgtaataa
     3001 ccctgtatct ttaacacctc tttgttcatt aggtgaatac atgtcgataa gtgtttcatt
     3061 gataaaaata gcttgagttt ctgttatata aattgtcatt tcggttttcc tactttcctc
     3121 cacgcctgaa ggataattat ctgtttacca aatctttaaa tgcaccgtca aatttattga
     3181 tggttctgtt taacgattcc aagaacttag gattgagatc ctctggcata gaaatttttt
     3241 tggatttttt tataacgatt tgttcgtttt cttcgttaat ttctaaactt acaacgtcac
     3301 cttgtttgat atttagagta gtgtaaaatt cttgcggtaa tgttaaacct aaagaattgc
     3361 cgattttagt aactttacgt tcgatttttt gtgtttccat gtggcgagtg tttgctgtca
     3421 tttggacttc ctccttggaa tttgccacac tattttttgt cgtagtctaa ttgccacttt
     3481 gtgtttgtat tgagtttttc gaattatata gtgtgatttt atgtttgttt tgtttgtttg
     3541 cttttggttt tttcggtctt cttagttttc tcttttgttt gttgtgttta gtctttctgt
     3601 attattatga cgtttagtta gaatgttata acagttataa cgtattaacc gtcaatacaa
     3661 ttatactcat ttttagaaat atgtaaacaa atttatattt cattgcaaaa gatagggaag
     3721 attgtagagc cttgcttgaa tttgttttat ttagacaaag aaagtgtaaa gggagttacc
     3781 tgttattcta attagcaggt aaagttcgag aatttctcga ttttttcatg gcaacacttc
     3841 gaacgtgttc tgaaatatat gtttttgagg tataggggat tgatgtgtaa ttcttaaaga
     3901 gaaaatatta taagttgttt gaaaaacgct tagaagtgaa aatgaagagg ctgagagata
     3961 ggaataattt ttgtttttaa aagaaaaaat gcccttgtcc acgaagtgga ttgaagggca
     4021 tttttttatg gtttttcgga cacgaaaaat acggttttct tctttcttta atacataggt
     4081 aactatttcc gcacggaaat tgatgcctat gtattctttt atcccttgat acacaaggga
     4141 tttcgaaaaa aaagacttgc tatttccggc gggtagattt atcattttta gtgtcgaatt
     4201 aaaaacaata actaccaagg aaggagcaag tccgaaaaag ggattcgtgt accctttttt
     4261 aagcaattca tataggacta atatatcaaa catgacaaag aaaaatcaag atgaaatcac
     4321 tggtaaatta caacctaaaa agaaacagaa tataagaatt tatgaattta tagagaagaa
     4381 aatgagtgag agcgggagag aattgtttaa atcgtgcagt acttttaatg agtttgtggc
     4441 tacgaaagat aaaaagaaaa agaaacgagt aaagggtaat gattgtaaaa atcgattttg
     4501 tccgatttgt gcttggagaa aagcagggaa ggacgcggtg aaaatcgcaa caatgatgga
     4561 agcaatcaag attgaagaaa agaaagagtt tcttttctta acattgacaa ctccgaatat
     4621 taaagctgat acggtaaaaa gcgagattga tagatttaat aaagctttta ataaattatt
     4681 taagagacgt aatattcaac gttctattaa gggttatatt agaaagctag aaatgacgta
     4741 tgataaagaa cgattcatca ctgaagaaat gtataacaac aaaaagagaa aagcatatta
     4801 tgattctcgt ggattgaaag tagacgatca taacccgaat tatgatacat ataatcctca
     4861 tttccatgta ttactttgtg ttgaaaaaaa ttattttaaa cgtaaagagc tttatattaa
     4921 gcaggaagaa tggttggaaa tgtggcgaga agtaacagat atgccagaga ttacgcaagt
     4981 acatattcaa aaagtagagt taattcgaga aggaaatgca gttgcagagg ttgcgaagta
     5041 ttcagcaaaa gattatgaaa tgtcagtgag tcaagatgtg ttcgacgtgt tttatttagc
     5101 tttaaaaggt cgacaattaa ttgtgtacgg tggattgcta aaagattatg ctaaaaaata
     5161 cgaagatggc gaattggaca agtataagag tacagataaa aatgagtatt attatagatt
     5221 aattgcaatg tggaataagg atttaatgaa atttgaacaa gaatatcaag agttaacgga
     5281 aagtgagaaa caagaatata acggtcattt gattgatgaa atggaggttg aatagttttg
     5341 tataaaaaag gtgatattgt taaacatacc tgttgggaat gttataacga agaaatgaaa
     5401 atttatcgga tttcaaaaag taaggtcaaa gatggtgaag atgtatatta ctcatataag
     5461 tgctaggtta aatgtgaaaa atgtggtgcg gttgatgata tttgaatgac aaagagagaa
     5521 ccagtcttaa tattaagttt ggttctctct tttttgtatt gaaagaaaac cttctgagtg
     5581 cagcaagtag cattcaaaag gtttttatga tctaacagag aattaacaaa aaagagtttt
     5641 ttcttaaatt taaatttgtg aatttgtgaa tttattttta aagtttattt cgagatatat
     5701 tttgttctga tgatttcgtt ttactactgt acgagttaaa aacctatagt gtgcgcatga
     5761 atttttttag atatatctat atcgataaaa taccgcacgt tttttgatgt tttttgaaaa
     5821 tagaaattct acatttttgg ggttgatttt ggatttttgg tgtgggattt tatttaaaaa
     5881 tttttaaatt tgaaatcttg atttttcgat ttttgaaatt tttgttttcc gttttctctg
     5941 taattctgta ttccgaaatt agggagttta tatagtacct ctatgacgaa ctgaaaacga
     6001 aaaaactttg ctcgcatatt taattcatag atatttcata tcgttaaaaa gttgcaccgt
     6061 tttaaacaag ttgtgtgata aaaaaagatg tttagattat gtttgaattt gttgtttctg
     6121 tgaatttttt atttcgtgat tcctgtatat ttttaatcct ttttactctt aaaatctttt
     6181 tgcttttgtg cgcaaatgaa aaattcaaaa agccacgcgt aacctttatt aaatatataa
     6241 atatattatt aaaataccta acccttttat gatgaaaaaa tataaatagt taaagaaaaa
     6301 agactgatga aaatatcagt cttttttctt tagttttatt tataaagagt atctctttat
     6361 ggtgttttct actttttctc tccagttctc tatatcgtca attgatattc cgatctttat
     6421 taattcgttt ggaatgaaac tgttatctaa aatccataaa gtgagccttt ctaatgtgat
     6481 tctttcgttt ccaatcgaat aaatacgttc actttgtttg caggtatatg gttttttacc
     6541 aacatatttt tctacataaa agtatctttt tccgtttgct ttgtaatttg ttagaaatgc
     6601 catagtaatg ctcctttcag gtgtttttat ggttagttaa agagtagttc tgtaatgttt
     6661 tttcaacttt ttcataccaa tatttcacat cgtttatgga tactccgatt tctagtagtt
     6721 cttttggtat acggttgtta tctaatagcc acattgcgat tctttctaaa gctatttttt
     6781 gattgccgat tgcgtaaatg catttatatt tgtattgctt tgagtaatat ggttgtgttc
     6841 caacgtattg ggctacataa aagtaaatgt ttccttttac tttttttgat attatatatg
     6901 ccaaagaaat gacctccaat cattaaaaat ctatattact ttgattaata ttaactttat
     6961 caggttaatt tttcaacaaa taaagtatga aataatgcat attttgaacg ttttaattta
     7021 aataggtttt aaattagttt aaataaataa aataaataac cttatatgta tataaattaa
     7081 ctttaattta aatgaagtat gaaatagtgc ataatatgat tttttattcc atacaggttt
     7141 aaaaatgttt tataaagtgc taaagttgtg tttaaaaggt taaaaaacaa ctattctttg
     7201 cacaaaagtg cccttataag aaaaaagcat gtatatttca tgagaaaaag gagtataaaa
     7261 aatgaaagta gtaaataact tccgtgtgat tatggcggta aagaatatag ataatgttgc
     7321 agagttggtt aggatgacag gggtaagtcg taactctatc aataagttgt ggcgtaacga
     7381 caatgtgtca tctttacgaa tagatacatt gctaccgatc tgtgaaaagt taaatgttaa
     7441 actatcggat ttaattgaat atattccaga cgatagtgag acaaaataat ttaggggcta
     7501 ttctatcttt agatagcttg tgtatgatga aatcggtatt aatatttgct agtggaaaac
     7561 aggttatatt cgcatatggt ttttggtagg gttatatgct tattttaaat ttcggcaaac
     7621 ttttatgact gtctt
//
