LOCUS       NZ_CP083124             8513 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis strain SA11 plasmid pCH_164-h, complete
            sequence.
ACCESSION   NZ_CP083124
VERSION     NZ_CP083124.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214337
            Assembly: GCF_020809145.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x, 70x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809145.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 13:17:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,862
            CDSs (total)                      :: 6,710
            Genes (coding)                    :: 6,385
            CDSs (with protein)               :: 6,385
            Genes (RNA)                       :: 152
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 104 of 325
            Pseudo Genes (incomplete)         :: 245 of 325
            Pseudo Genes (internal stop)      :: 76 of 325
            Pseudo Genes (multiple problems)  :: 76 of 325
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083124.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="SA11"
                     /isolation_source="Biopesticide Delfin"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_164-h"
                     /collection_date="2017"
     gene            join(8201..8513,1..767)
                     /locus_tag="K8Z41_RS33940"
                     /old_locus_tag="K8Z41_33870"
     CDS             join(8201..8513,1..767)
                     /locus_tag="K8Z41_RS33940"
                     /old_locus_tag="K8Z41_33870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102658.1"
                     /translation="MNVQLGNEQITKLLNDWYQEIRAQHIIKAKQLKQKIEKEINNIEE
                     DQNLLLYYSLIDLRYKILTNDYADREQSLEKIEQLKEHTNNFLDYYYHFFKGMHAMKTG
                     NYSETQKYYDIAEKLLEEIPDEVEKAEFNYAVATFYYHTHQALLATQYANKAKSFFSGK
                     LGYEIKTGACENTLGMACITLREFSVAEEYLLSAINKFEKHSENKLALIVRYNLGLLYA
                     DQNLSELAIRYLLESFENQEDDYKTMFLLAQEYYKLKQTNTVNTYIEKGLNVCNQEYKI
                     HFLFLQALNNKLSTEQLEKVMLDAIPYFEKQNLWKYIQDYTEELAIRFYEEKNKDKSNE
                     YFYKSYKAKRNLLERESLK"
                     /name="RapH N-terminal domain-containing protein"
     gene            764..910
                     /locus_tag="K8Z41_RS33945"
                     /old_locus_tag="K8Z41_33875"
     CDS             764..910
                     /locus_tag="K8Z41_RS33945"
                     /old_locus_tag="K8Z41_33875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000976019.1"
                     /translation="MMKKFSLILIGVACTTGIFFSQFNNSIQTHDAKEKNDIIQQYAHG
                     KDI"
                     /name="hypothetical protein"
     gene            1030..1611
                     /locus_tag="K8Z41_RS33950"
                     /old_locus_tag="K8Z41_33880"
     CDS             1030..1611
                     /locus_tag="K8Z41_RS33950"
                     /old_locus_tag="K8Z41_33880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000723966.1"
                     /translation="MKKISIERSNWRKTYKKMIPVLLVGGILAGNVAFVTNNVFAATTN
                     NNNIIQTKQSLVSGQDEKSINELAEAFQFMHEEAAIYNNQGEIVDFDFNKIEAKYGNDV
                     GLDALKQQIKKDKSDIAGLSFTSCMKDALYDFFGVNAVQAAFNGGLAYYLEKKLWKEAA
                     KIGVKFFIGSSVAGLAATLAYYGGKCAIYG"
                     /name="hypothetical protein"
     gene            1604..1801
                     /locus_tag="K8Z41_RS33955"
                     /old_locus_tag="K8Z41_33885"
     CDS             1604..1801
                     /locus_tag="K8Z41_RS33955"
                     /old_locus_tag="K8Z41_33885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000361625.1"
                     /translation="MDKSSILKQMYFILFPVTLFLSLNIYQWIVKSDYSFTGYIIQNLI
                     IVALALVAAIIAYLITKKNI"
                     /name="hypothetical protein"
     gene            2653..2823
                     /locus_tag="K8Z41_RS33960"
     CDS             2653..2823
                     /locus_tag="K8Z41_RS33960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001218510.1"
                     /translation="MALSLLGLIVFKKSWLVAEKNREDWRGCIAWQRDMLCSRGEYKKR
                     LFNTWMSTVKS"
                     /name="hypothetical protein"
     gene            3174..4379
                     /gene="mobV"
                     /locus_tag="K8Z41_RS33965"
                     /old_locus_tag="K8Z41_33890"
     CDS             3174..4379
                     /gene="mobV"
                     /locus_tag="K8Z41_RS33965"
                     /old_locus_tag="K8Z41_33890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033305.1"
                     /translation="MSKFVICHMQKFKMRDVKGLQIHNQREKESHSNSDIIQARTEQNY
                     DLIHDKEKVDYKKLIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQVEQRRF
                     FEESLRFLKGRYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSEFHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEELEQEKKGIDSRLHDLKM
                     SLDQAKSVDEIPVKEKGGFIRSKTVEITSEDFESIKVLARSSEALREDVRRLKNESGVI
                     TREKDGLYREKCILEGQITELKRENRGLKEENDFFKKTLERVKELYKEKLPELAGVIGY
                     VKGSILDRMNCKFLKRYFTSDDEVRGAQKFLNHKQEHEEQQKRLKQVRSSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            4849..5895
                     /locus_tag="K8Z41_RS33970"
                     /old_locus_tag="K8Z41_33895"
     CDS             4849..5895
                     /locus_tag="K8Z41_RS33970"
                     /old_locus_tag="K8Z41_33895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001085408.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIKKKMSESGKELFKSCSIF
                     NEFLATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQEVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELSESEKQEYNGHL
                     INEVDVEA"
                     /name="protein rep"
     gene            5899..6096
                     /locus_tag="K8Z41_RS33975"
                     /old_locus_tag="K8Z41_33900"
     CDS             5899..6096
                     /locus_tag="K8Z41_RS33975"
                     /old_locus_tag="K8Z41_33900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861196.1"
                     /translation="MKVAGDLYYYCLGCKKFHEYEKIDHKGVNRKLCFYCFKIQSKKTK
                     IIGDAEGRMQICETCHKELF"
                     /name="hypothetical protein"
     gene            complement(6965..7228)
                     /locus_tag="K8Z41_RS33980"
                     /old_locus_tag="K8Z41_33905"
     CDS             complement(6965..7228)
                     /locus_tag="K8Z41_RS33980"
                     /old_locus_tag="K8Z41_33905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874967.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISINDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            7502..7729
                     /locus_tag="K8Z41_RS33985"
                     /old_locus_tag="K8Z41_33910"
     CDS             7502..7729
                     /locus_tag="K8Z41_RS33985"
                     /old_locus_tag="K8Z41_33910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869869.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869869.1"
                     /translation="MKVVSNIRMIMAKKNIDNISDLVRITGVSRNSINKLWHNENVSSL
                     KLDTLITICEKLDVELLDLIEYIRDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          4825..5877
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.934"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3175..4376
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attatagtga aactcaaaaa tattatgata tagcagaaaa attactagaa gaaattcctg
       61 atgaagtaga aaaagctgaa tttaattatg ctgttgcaac tttctattat catactcatc
      121 aagccttatt agcaactcaa tatgctaaca aagccaaaag tttttttagt gggaaactag
      181 gatacgagat taaaaccgga gcatgtgaaa acacacttgg tatggcttgt attacattaa
      241 gggaattttc tgttgcagaa gaatacttgc tttccgcaat taataaattt gaaaaacaca
      301 gtgaaaacaa attagcttta atcgtacgtt ataacttagg tcttttatat gcagatcaaa
      361 atctttctga acttgcaatc cgttatcttc ttgaatcctt tgaaaatcaa gaagacgatt
      421 acaaaacaat gtttttatta gcacaagaat attacaagtt aaaacaaaca aatacagtaa
      481 acacatatat tgaaaaaggg ctaaatgttt gtaaccaaga atataaaatt catttcttat
      541 ttttacaagc attaaacaat aaattatcta ctgaacaatt agaaaaagtc atgttagacg
      601 cgattccata ttttgaaaaa caaaatctat ggaaatacat acaagattat actgaagaat
      661 tggctataag attttatgaa gaaaagaata aggataaatc taatgaatat ttttataaaa
      721 gttacaaagc taaaagaaac ttattagaaa gggagtcatt gaaatgatga aaaaatttag
      781 tctgatacta ataggggtag catgtacaac aggtattttc ttttcccaat tcaataattc
      841 cattcaaaca catgatgcta aagaaaaaaa tgatattatt caacaatatg cacatggaaa
      901 agatatttaa aagcaaattc caacaattag caatcgacct aaatattagg attattaaca
      961 aaatattgaa ttatatgtaa attaagttta ttatctattt tgaataataa acttaagggg
     1021 atgaaattta tgaaaaaaat tagtattgag agaagtaatt ggagaaaaac atataaaaaa
     1081 atgattccag tattgttagt cggaggaata ttggcaggaa atgtagcatt tgtgacaaat
     1141 aatgtttttg cggcaactac aaataacaac aatataatac aaacgaaaca gtctctcgtt
     1201 tctggtcaag atgaaaagag cattaatgaa ttagcagaag cgtttcaatt tatgcatgaa
     1261 gaagccgcta tatataataa tcaaggtgaa attgttgatt ttgattttaa taaaatagaa
     1321 gcgaaatatg ggaatgatgt tggtttagat gcacttaaac aacaaattaa aaaagataaa
     1381 agtgatattg ctggtttatc ttttacaagt tgcatgaaag atgctttata tgactttttt
     1441 ggagtaaatg ctgtacaagc cgcatttaat ggtggattgg catattatct tgagaaaaaa
     1501 ttatggaaag aagcggctaa aataggcgtt aaattcttta taggaagtag cgtagcaggt
     1561 ttagcggcta ctttagctta ttatggaggt aagtgtgcaa tttatggata aaagttcaat
     1621 tttaaaacaa atgtatttta ttttatttcc ggtaacactt tttctatctc taaatattta
     1681 tcaatggatt gttaaaagtg attactcatt tacaggatat ataatccaaa atttaattat
     1741 tgtagcatta gcgttagtag cagctatcat agcttattta attactaaaa aaaatattta
     1801 acacataatg ttagtctaaa taacagaaaa ttctaaccct tattcttatt ttgtattata
     1861 ctggttatgt acaggtaaaa tggtttacaa gttaattttt cctcgtttca aaaaaagtcc
     1921 attcttttca aaagaatgga cttttttctt gttatatttg ctgtaactat taaaagggca
     1981 tcaaaacgat ctacaaaagc ctcaagacga cccacaaacc atctttaaac ggtctcaaaa
     2041 cgaactacaa cccgaaaaaa cgcatcccag tttttttagg tttaatttta agcgcatacc
     2101 aagcgcatat aggcgcatac gggacacata caaagtgccg aaaaacgcat ataggtcgca
     2161 tacaaacggt agaaatacat ccctaaaaat agagtgatag aaacccaata ggcacagcct
     2221 atttcaccac tttttttgca aagtgggatt cggtttcctt atgctctttt tttatttttg
     2281 ctttttaagg gtcttataat cgtttttaag tattttagga tgttgggttt cttaagggaa
     2341 ctagaagttc ctagaatgga ttctgagcgt tctgaggtgt atttttctat ggattcgata
     2401 ttggaattgc tctaggaata gttgtcgttt tattctgtgg ctgttccgac agtggttctc
     2461 ataaagaata tgattgtatc aatagatttg atgatgtttg aaaaatagag aatagttgaa
     2521 gctcttcctg taaaacaaaa aaaattatgt ttgtagagga agtagtcatt tcactacaag
     2581 aaaattccca tagatcggtg gcgagttgaa aaatgttgaa ggttggcgag gacattttac
     2641 gaggacgcgg gaatggcttt gtcattgttg gggttaatag tttttaagaa aagttggtta
     2701 gtagctgaaa aaaataggga agattggcga ggatgtattg catggcagcg ggacatgtta
     2761 tgtagtcgtg gtgaatataa aaagagactt tttaatacat ggatgtcaac tgtaaaaagt
     2821 tagaggtagt tgtatgatgt ttgtccaacg gtaagcggaa cgaagtgagc attaaggagt
     2881 ggacgaaccc actgtgccca gaaccctctt ggaactccaa aacgttagcc gttttgggga
     2941 tgccaaccgg cgagggagtc cctcaaggat tgaggggttg gggagttcga ttgaaagggg
     3001 tcaaggggaa gagtttccct tgtgggtttg ggcaaagccc atgatttttt ttgccatgct
     3061 tgttatggct cggcggtagc cgaaatgtcc cgaagggcac acgtgtttac gcagtaaact
     3121 gtagtgtgct actgtttaca tggaactacc cccgaaaaaa aggagcgagg cccatgtcga
     3181 agttcgtgat ttgccatatg caaaaattta agatgagaga tgtgaaagga ttacaaatac
     3241 ataatcaacg tgaaaaagaa agtcattcca attcagatat tattcaagca cgaacggaac
     3301 agaattatga tctgattcat gacaaagaaa aagtggatta taagaaatta attcaaaatc
     3361 gaattgataa tggtgttgta tctaatcgtg caattcggaa agacgctgtt gtttgttgtt
     3421 cgttcatgat ttcggctagt ccggattata tgaattcttt atctcaagtg gaacagagac
     3481 gattctttga agagtctctt cgttttttga aggggagata tggagaagag aactttgtgt
     3541 atgcttccgt acatgttgat gaaaaaacac ctcatatgca tgtgggtatg gttccagtta
     3601 atgaaaaaca aaaactatca gcgtattcat tttttaaaaa caaatcagag tttcatgatt
     3661 tacaagataa aatctatgaa catgtgaaag aaaagggatt tgatatagag cgtggtgttt
     3721 ctagtgatag aaaacattta tcgacgcagc ggtttaaagc ggtgacttta caacaagaaa
     3781 tagaagaact agagcaagaa aagaagggaa tagatagtcg tcttcatgat ttaaaaatgt
     3841 ctttagatca agcaaaaagt gtggatgaga tcccagttaa agaaaaaggt gggtttatac
     3901 gctctaaaac ggtcgagatt acctcggaag acttcgaaag tataaaagta ttagctaggt
     3961 cgtcggaagc ccttagagaa gatgttagac gccttaaaaa cgaatcaggt gtaattacac
     4021 gtgaaaaaga tgggctgtat agagagaaat gtattttaga ggggcaaata acagaattga
     4081 aaagagagaa tcgaggattg aaggaagaga atgatttctt taagaaaacg ttggaacgag
     4141 tgaaagagtt gtataaagaa aagcttcctg aattggctgg ggtgattggt tacgttaagg
     4201 ggagtatatt ggacaggatg aattgcaaat ttttaaaacg atattttact agtgatgatg
     4261 aagtgagagg cgctcaaaag tttttgaatc ataaacaaga gcatgaagaa caacaaaaga
     4321 gattaaaaca agtgcgaagt agccaacaga aaaatcggga ccagggattg gagagatagt
     4381 gtggttctgt ttgagttttt tgttttttag atttatagcg ttagcggaat ttgtttttta
     4441 taacttgctt aagatgagta tgtttctcag atgttttatc cgttaaaaaa aatggttttg
     4501 aacgaaaaaa aatgcccctg tccacggagt ggatttaggg gcattttttt atgatttttc
     4561 gtgttcgaaa aatcgagttt tttctttctt taatacatag gtaactattt cggcaacgaa
     4621 attgatgcct atgtattctt ttagccctta tgtatcaagg gtttgtaaaa aaaaagactt
     4681 gctttttcag gcggatagat ttatcatttt tagtgtcgaa ttaaaaatca taaataccac
     4741 gagaggagca agtccaaaaa aaggattcgt acaccctttt ttatatcgaa tatgaaattc
     4801 agaaattaga aattctgatg tttttcagat aggactaata tatcaaatat ggcaaagaaa
     4861 aatcaagatg aaatcactgg taaattacaa cctaaaaaga aacagaatat caagatttac
     4921 gaatttataa agaagaaaat gagtgagagc gggaaagaat tgtttaaatc gtgcagtatc
     4981 tttaatgaat ttttggctac aaaggataaa aagaagaaga aacgagtaaa ggggaatgat
     5041 tgtaaaaatc gattttgtcc gatttgtgct tggaggaaag cagggaagga cgcggtgaaa
     5101 atcgcaacga tgatggaagc aattaagatt gaggaaaaga aagagtttct ttttttaacg
     5161 ttgacgactc caaatattaa agctgatatg gtgaaaagtg agattgatag atttaataaa
     5221 gcttttaaaa agttatttga tagacgtaat attcaacgtt ctattaaggg ttatattaga
     5281 aagctagaaa tgacgtatga taaagaacga ttcatcactg aagaaatgta taacaacaaa
     5341 aagagaaaag catattatga ttctcgtgga ttgaaagtag acgatcataa cccgaattat
     5401 gatacatata atcctcattt tcatgtattg ctttgtgttg ataaaaatta ttttaaacgt
     5461 aaagagcttt atattaagca agaagaatgg ttagaaatgt ggcgagaagt aacagatatg
     5521 ccagagatta cgcaagtaca tattcaaaaa gtagagttaa tacgagaagg aaatgcagtt
     5581 gcggaggttg cgaagtattc agcgaaagac tatgaaatgt cggttagtca agaagtgttt
     5641 gacgtgttct atttagcatt aaaaggtcgt caattaattg tttacggtgg attattaaaa
     5701 gattatgcga aaaaatatga agatggcgaa ttggacaagt ataagagtac agacaagaat
     5761 gaatattatt atagattaat cgcaatgtgg aataaggatt taatgaaatt tgaacaagag
     5821 tatcaagagt tatctgagag tgagaagcaa gagtataacg gtcatttaat taatgaagta
     5881 gatgtggagg cgtagtgtat gaaagtagcg ggagatttat attattattg tcttggttgt
     5941 aaaaagtttc atgaatatga aaaaattgat cataaaggtg taaatagaaa attatgtttt
     6001 tattgtttca agattcaatc taaaaaaacg aaaattattg gtgatgctga aggtagaatg
     6061 caaatttgtg agacttgtca taaagaattg ttttagttgg ttgatttgtg aaaaaagcaa
     6121 agtcaaatgt tagaaatttt taataaggga actgactgtt atatcaagtc agttccctta
     6181 ttttattgag tctatttttg agtgcagcaa gtagcattca aaagtttttt tatgatttaa
     6241 caaaaaggag tttttcttaa atttaaattt cgtgaatttg taaatttatt tttaaagttt
     6301 atttcgagat atattttgtt ctactaattt tgttttacta acgtacgagg tgaaaactta
     6361 aagtgtgcgc atgaattttt ttagtaatat atattacgat aaaataccgc acgttttttg
     6421 atgatttttt tgaaaatata gttttgagaa attctaaatt ttcggggctg attttggatt
     6481 ttttatgtgg gatttgattt tggaatttgt taaattggaa atctcgattt ttcgattttc
     6541 ggaatttttg ttttctcttt tctttgttat tctgtatttc gaaattaagg agtttatata
     6601 ataccttcat gacgaactga aaacgaaaaa actttgctcg catatttaat tcatagatat
     6661 ttcatatcgt taaaaagttg caccgtttca aacgagttgt gtgataaaaa gatgtttaga
     6721 ttatgtttga aattgttgtt tctgtgaatt ttttatttcg cgatttctgt atatttttaa
     6781 tcctttttac tcttaaaatc tttttgcttt tgtgcgcaaa tgaaaaaatc aaaaagtcac
     6841 gcgtaacttt tattaaatat ataaatatat tattaaaata cctaaccctt ttatgatgaa
     6901 aaaatataaa tagttaaaga aaaaagactg ataaaaaata tcagtctttt ttctttagtt
     6961 ttatttataa agagtatctc tttatggtgt tttctacttt ttctctccag ttctctatat
     7021 cgttaattga tattccgatc tttattaatt cgtttggaat gaaactgtta tctaaaatcc
     7081 ataaagtgag cctttctaat gtgattcttt cgtttccaat tgaataaata cgttcacttt
     7141 gtttgcaagt atatggtttt ttaccaacat atttttctac ataaaagtat ctttttccgt
     7201 ttgctttgta atttgttagg aatgccaaaa tgataatctc ctcttagttt ttttagagtt
     7261 aaaaatacat tttaaaagat taaattttaa gttatatatt taattttaac tctaaggggt
     7321 taaaatttca attaaaacaa ataaaattaa ataataaaac aatatatttt atttttaagg
     7381 ttaatattaa atgtgaatat ataaaaaata accttaatat tatttaattt atgttttatt
     7441 tgtataaaag tatccttgta agtaaaatgg tatgtacatt gaatgaaaag gagtatgaaa
     7501 aatgaaggta gtaagtaata ttcgtatgat tatggcgaaa aagaatatag ataatatttc
     7561 ggatttggtg agaataacgg gagtaagtcg taattctatt aataagttgt ggcacaatga
     7621 gaatgtgtct tctttaaaat tagatacatt gataacgatc tgtgagaagt tggatgttga
     7681 gttattggat ttaattgaat atattcgaga tgatagtgag acaaaataat ttaggggcta
     7741 ttttagcttt agatagcttg tgtatgatga aagcatattt ttgctaatcg acaatgagtt
     7801 atattcacat atgttttttt tgtagggcta tatgtgtatt taaagttttg atttctattt
     7861 gttattgtat gaatatggtt tgaatgtggt aaatgaaatt atagtcttgc taggtgcgtc
     7921 aacaccttgg aagatgagcg gtactggcat acttcgtgat ttcattgata gtagtgcagt
     7981 aaagtgatgg aattgatgag tattgaaaaa gagttcctgt ttgggggctc tttttttatt
     8041 tgttactaag tatttttaaa tagtgaaggt gctacactat ttaaaaatac agaatattaa
     8101 aaactttata tatcaactgt tgaaaagtta gtcttataca atagcatgat aacatgatag
     8161 aaaataaaat atagataatt ttggaaaagg agaagattct atgaatgttc aactaggaaa
     8221 cgaacaaatt acaaaattat taaatgattg gtatcaagaa attcgagcgc aacacattat
     8281 taaagcaaaa caattaaaac aaaagattga aaaagaaatt aacaacatag aagaagacca
     8341 aaatttactt ttatattatt cacttattga tttgagatac aagattctta caaacgacta
     8401 tgcagataga gaacaatctt tagaaaaaat cgaacaatta aaagaacaca caaataattt
     8461 tttagattac tattatcatt tctttaaagg catgcatgca atgaaaacag gaa
//
