LOCUS       NZ_CP083115             2062 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain ABTS-351 plasmid pCH_183-n, complete
            sequence.
ACCESSION   NZ_CP083115
VERSION     NZ_CP083115.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214338
            Assembly: GCF_020809105.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x, 70x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809105.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,043
            CDSs (total)                      :: 6,891
            Genes (coding)                    :: 6,549
            CDSs (with protein)               :: 6,549
            Genes (RNA)                       :: 152
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 116 of 342
            Pseudo Genes (incomplete)         :: 252 of 342
            Pseudo Genes (internal stop)      :: 75 of 342
            Pseudo Genes (multiple problems)  :: 77 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083115.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="ABTS-351"
                     /isolation_source="Biopesticide DiPel DF"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_183-n"
                     /collection_date="2017"
     gene            join(1726..2062,1..533)
                     /locus_tag="K8Z42_RS35010"
                     /old_locus_tag="K8Z42_34965"
     CDS             join(1726..2062,1..533)
                     /locus_tag="K8Z42_RS35010"
                     /old_locus_tag="K8Z42_34965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198524.1"
                     /translation="MQVYLDRLMIKYKDVTEKQFSDVLTKISSKQIFLPNTPIRSEHGT
                     SVRDYHRVIHIGYGEGAVYIGWKHNSEKEKDSYDMKVDFNPSKFENNELQKDSYEKVFE
                     TVFHTLNAVLKSNKRVVYGMDIAFDIERHMSDIVSYSKTGKQQDRHKGTVYYGNRNKDG
                     YLKIYDKKKELYNHFKRMIEEENLTRIEYSWRDSDGVVVDEIRKSPPFSIDESYTFSIF
                     NLNNVKGALKACLICYSNGTMDMKEFPRRTKESIKKALEEMDHLAVDPILQDCWLSILE
                     NIKNYTRL"
                     /name="hypothetical protein"
     gene            721..963
                     /locus_tag="K8Z42_RS35015"
                     /old_locus_tag="K8Z42_34970"
     CDS             721..963
                     /locus_tag="K8Z42_RS35015"
                     /old_locus_tag="K8Z42_34970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001293418.1"
                     /translation="MSEMVRVNTRISKKLNDWLDEYSKESGVPKSTLVHLALENYVNQK
                     VMLEQMPKMQQMLSMMFENVTQQQLNQKGNMFELK"
                     /name="hypothetical protein"
     gene            1398..1625
                     /locus_tag="K8Z42_RS35020"
                     /old_locus_tag="K8Z42_34975"
     CDS             1398..1625
                     /locus_tag="K8Z42_RS35020"
                     /old_locus_tag="K8Z42_34975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002100596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000572905.1"
                     /translation="MIESEGNNLKLTERQLNDLKRISELRVKLFGVPGESVVDPENVEF
                     LLDNAISSYLGQLEIFEVTIEIEQYNSMCG"
                     /name="hypothetical protein"
     Typing          1727..2062
                     /name="rep_cluster_1434"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3e-175"
ORIGIN
        1 ttttgaagtc taataagcga gtggtttatg gtatggatat tgcttttgat atagagcgtc
       61 atatgagtga tattgtgtct tatagtaaaa caggaaagca acaggataga cataaaggaa
      121 ctgtttatta tggaaataga aataaagatg gatatttgaa gatatatgat aagaaaaagg
      181 agttatataa tcattttaaa agaatgatag aagaagagaa tttgactcgt attgagtata
      241 gttggagaga ctctgacggt gtagtggtag acgaaataag gaagagtcct ccgtttagta
      301 ttgatgaatc ttatacattc tcgattttta atttgaataa tgttaaaggg gcattaaaag
      361 cttgtttgat ttgttattct aatggaacta tggatatgaa agagttccct cgtagaacta
      421 aagagagtat aaaaaaagcc cttgaagaaa tggatcactt ggcggtggac cccattctac
      481 aggactgttg gttatctata ttagaaaata ttaagaacta tactcgttta tgatattagc
      541 gtgtgcttct ctgtgtgtca agagggtgtc aatatgatgc tctctttttg tttttcttaa
      601 cttgtttata ttaatagcgg atagagtccc acttttacat tgttctggtg tatcttagtg
      661 ttgatattgt gtttaaactg atgttatatt tatgtagtac gatatacaag aggtgattag
      721 atgagtgaaa tggttcgtgt taatacacgt atcagtaaaa agttaaatga ttggttggac
      781 gagtatagca aagaaagtgg tgtaccgaaa agcactttag ttcatttagc tttagagaat
      841 tatgtgaatc aaaaggttat gttggaacaa atgccaaaga tgcaacaaat gttgagtatg
      901 atgtttgaaa atgtaacgca gcaacaattg aatcaaaaag ggaatatgtt tgagttgaag
      961 taacggttat gtttcgaaaa tgtagtctta ttgattagga gatgcacatc gataaactaa
     1021 atgcgtagtt ggtgtggctg aagtttgccc gccacctact catttagaat atccgtgcat
     1081 gggtccctga acaattagga aacggctttt gtaatcgtat gagttgtaat tattattcac
     1141 ttgggtgaac gttgtatgac tgaaaggaaa cccatggcag ttaactgtgt aaatgggcac
     1201 ttatttatta agtgtattcg acggagccgg aagtagacga cttaagggag cgacatgaat
     1261 gaagtgaagt gcagacagaa ggtaaggtga cggagtggac tctcaaagga cacgacggcg
     1321 cgtgaactga tggcaaacga aatggaatga gtgagaagag cgtacggtcg gcgaggtaac
     1381 ggaggtgtag gagcagattg atagaaagtg agggtaacaa tttgaaactg acagaaagac
     1441 aattgaatga tttgaaaaga attagcgaat tacgtgtaaa gttgtttgga gttcctggtg
     1501 aaagtgtagt tgatccagag aatgttgagt ttttattgga taatgctatt agttcttatt
     1561 tagggcaatt agaaattttt gaagtcacga tagagattga acagtataat tcaatgtgtg
     1621 ggtaaattgt agaaatgtgg cgaagacatt ttcggacatt ctaatagccg aaaatcgtgt
     1681 acaaaatgac atgtttaata aaaaataagg agcgggatag attttatgca agtttatttg
     1741 gataggctaa tgattaagta taaagatgta acagagaaac aatttagtga tgttttaact
     1801 aaaatatcgt caaagcagat ttttttaccg aatacaccta ttaggtcaga acatgggacg
     1861 tctgttagag attatcatag agttatacat attggatatg gtgaaggtgc agtttatata
     1921 gggtggaaac ataattcgga aaaggaaaaa gatagctatg atatgaaagt tgattttaac
     1981 ccttctaaat ttgaaaataa cgagttgcaa aaagatagtt atgaaaaagt gtttgaaacc
     2041 gtttttcata cgttaaatgc ag
//
