LOCUS       NZ_CP083114             5120 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain ABTS-351 plasmid pCH_183-m.
ACCESSION   NZ_CP083114
VERSION     NZ_CP083114.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214338
            Assembly: GCF_020809105.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x, 70x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809105.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,043
            CDSs (total)                      :: 6,891
            Genes (coding)                    :: 6,549
            CDSs (with protein)               :: 6,549
            Genes (RNA)                       :: 152
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 116 of 342
            Pseudo Genes (incomplete)         :: 252 of 342
            Pseudo Genes (internal stop)      :: 75 of 342
            Pseudo Genes (multiple problems)  :: 77 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="ABTS-351"
                     /isolation_source="Biopesticide DiPel DF"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_183-m"
                     /collection_date="2017"
     gene            complement(67..321)
                     /locus_tag="K8Z42_RS34970"
                     /old_locus_tag="K8Z42_34925"
     CDS             complement(67..321)
                     /locus_tag="K8Z42_RS34970"
                     /old_locus_tag="K8Z42_34925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976760.1"
                     /translation="MDCKTLVKRYEEQNEELRKLNSILTKRIYHLLQENESLTSHVSFM
                     RDFNEELIRDNMNYLGDKLDEKLQDIEGDDSNDRHAKSN"
                     /name="hypothetical protein"
     gene            complement(439..591)
                     /locus_tag="K8Z42_RS34975"
                     /old_locus_tag="K8Z42_34930"
     CDS             complement(439..591)
                     /locus_tag="K8Z42_RS34975"
                     /old_locus_tag="K8Z42_34930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016090429.1"
                     /translation="MRDKLLDFIIELSQSSKQVVSKSYVIDRLMQVTKEDYKELEKKTE
                     NAEKD"
                     /name="hypothetical protein"
     gene            complement(605..805)
                     /locus_tag="K8Z42_RS34980"
                     /old_locus_tag="K8Z42_34935"
     CDS             complement(605..805)
                     /locus_tag="K8Z42_RS34980"
                     /old_locus_tag="K8Z42_34935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957403.1"
                     /GO_component="GO:0009289 - pilus [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="WP_000957403.1"
                     /translation="MLTPREQDTLECISGYMEEFGFAPSVRDMASRMYVSHKTAHRYLM
                     QLESKGRIRRVHQRPRAIQLL"
                     /name="FaeA/PapI family transcriptional regulator"
     gene            complement(822..3026)
                     /locus_tag="K8Z42_RS34985"
                     /old_locus_tag="K8Z42_34940"
     CDS             complement(822..3026)
                     /locus_tag="K8Z42_RS34985"
                     /old_locus_tag="K8Z42_34940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090430.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO:0000166 - nucleotide binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="WP_016090430.1"
                     /translation="MSTTNRKKRREIKLFTLDTETRGLDGDVFRIGLFDGKQYYTGYTF
                     ADVLPVFEKYKAYDCHVYIHNLDFDLSKIIAELRDYAEPTFNNSLFINGNIVTFTASHI
                     ILHDSFRLLPSSLENLCRDFDLLEGGKMDIVDYMEENNYGIYNVKNRKLNKRLTKGNFF
                     TTVDKDDPVLCEYMEYDCRSLYKILEIVIGLSKLEVEQFINCPTTASLAKTVYKEQYKK
                     DYKVAISTKQYNHKQLGKGLEAFIRKGYYGGRTEVFTPRIENGYHYDKNSLYPYVMKMA
                     EMPVGYPNVLDNEEAELSFDLWKRRRYGAGFIHAKVHVPEDMYIPILPKKDYTGKLIFP
                     VGKIEGVWTFPELALAEAEGCKIEKIESGVVFEKTAPVFREFISYFEEIKNTSKGAKRA
                     FSKLMQNALYGKFAMQRERIMYADISERDKLEAEGHTVSEIIYDMNGIRMEFLEYDGYA
                     MAEYIQPHISAYITSIARILLFKGLKYAHEKGILAYCDTDSCATTTKFPDKMVHDKEYG
                     KWKLEGYVIEGLYFQPKMYAEKAINTDGEYEEVLRMKGVPKWVVEEQLDYNSFRKWYLQ
                     VKRGKAEIPIYKGGERVQKFLTKSKNNIEMNELAEMHKTINFAREQKRNIDLNKNITSP
                     LVRNDYGENKDEKSEYEFDEWYERLEEFNDDMNAVEELCMKFGKIQIPEKKQRKLYGLY
                     KEYSSKAKAMCFSNEGLPIQDWCKKTGWDMKELLGELSFL"
                     /name="DNA polymerase"
     gene            complement(3013..3750)
                     /locus_tag="K8Z42_RS34990"
                     /old_locus_tag="K8Z42_34945"
     CDS             complement(3013..3750)
                     /locus_tag="K8Z42_RS34990"
                     /old_locus_tag="K8Z42_34945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016090431.1"
                     /translation="MANKRLKKKLETKRKKSLLVSEGYSKKETKKLKGRELETVYKKKA
                     HNRKNRERAREIANLARQWGLSPSKFNSWKKLLPEIERIKKEQDREAPFLVIYYQDFTG
                     ETDSKFIYDFKKRNNTRSRSQITRSIIGWLQNAQNKLFLGRVAMRIVPKRDVSKTNTLW
                     KNHGYVKIYEGQGKELTKLLTAIETIMVGVYDVKDRDKYLKQLLNNLRSLPYKQAHRNA
                     NEIQKIYDTKSYTKESWDNDEYY"
                     /name="hypothetical protein"
     gene            complement(3809..4033)
                     /locus_tag="K8Z42_RS34995"
                     /old_locus_tag="K8Z42_34950"
     CDS             complement(3809..4033)
                     /locus_tag="K8Z42_RS34995"
                     /old_locus_tag="K8Z42_34950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976756.1"
                     /translation="MNAIKNLVQVSIRTNERELEFVSFNGMVHLKQKLEEDETSVVLTK
                     EELDMVAAMSNFDNVIANFMNSITEKKAM"
                     /name="hypothetical protein"
     gene            complement(4105..4605)
                     /locus_tag="K8Z42_RS35000"
                     /old_locus_tag="K8Z42_34955"
     CDS             complement(4105..4605)
                     /locus_tag="K8Z42_RS35000"
                     /old_locus_tag="K8Z42_34955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976755.1"
                     /translation="MENNTKQLNNEVNEIAVVDEHVNTNDIVTVDKVSPSIFEADGAKD
                     VFFSSIQSKDRKSAIKVYNAINSSENALADHRGEVLEITDMVAHAITLQDELTKEDVDA
                     LRVVLIDKNGKAYHAVSQGVVSSIQKIISIVGPAPWNEPLEIVPAEVKTRKGFKTLTLQ
                     LQG"
                     /name="hypothetical protein"
     gene            complement(4621..4797)
                     /locus_tag="K8Z42_RS35005"
                     /old_locus_tag="K8Z42_34960"
     CDS             complement(4621..4797)
                     /locus_tag="K8Z42_RS35005"
                     /old_locus_tag="K8Z42_34960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976754.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_015976754.1"
                     /translation="MSKLLTAQEVADLLRVHKKTIHRMIHSGKLDASKVANKFLIKEED
                     AKGLLENKNKMDN"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 tcttcctcgt ttttcttacg cgcgaagaat tgtccgtcac cgaatcgtaa tagaaagggg
       61 ttttcttcaa ttgcttttag catgtctgtc atttgaatca tctccttcta tatcttgtaa
      121 tttttcatct agtttatcgc ctaaataatt catgttgtcg cgtattaatt cttcattgaa
      181 atctctcata aacgatacat gacttgttaa tgattcattt tcttgtagca aatgatagat
      241 tcgttttgta agaatagaat ttaattttcg tagttcttca ttttgttctt catatcgctt
      301 tactaacgtc ttacaatcca tctgtcacac tccttatcga atgtttgttc tgtatatgta
      361 tactataata cgaacaattg ttccgcataa cgtcacacgt gtgtcatttt ggacaaataa
      421 aaaagactag caactttgct agtccttttc cgcattttct gttttctttt ctagttcctt
      481 gtagtcttct tttgttactt gcattaacct gtcgatgaca tacgacttgc ttacgacttg
      541 tttgctagat tgtgatagtt cgatgataaa gtcgagcaat ttgtcacgca tacctttccc
      601 ctccttataa caactggatg gctctaggtc tttgatgcac tctcctaatt cgtcctttac
      661 tttccaattg cattaaatag cgatgtgctg tcttatgact tacgtacatt cgactcgcca
      721 tatctcgcac actcggtgca aacccaaact cttccatata cccgcttata cattctaacg
      781 tatcctgttc ccttggcgtc aacatttttc gtcactccct attataagaa acttaattcg
      841 cctaatagtt ctttcatgtc ccatcctgtc tttttacacc aatcttgaat cggaagtccc
      901 tcatttgaaa aacacatagc tttcgctttt gaagaatact ctttataaag tccgtataac
      961 ttcctttgct ttttctctgg tatttgtatc tttccaaact tcatacaaag ttcctctacc
     1021 gcattcatat catcgttaaa ctcttctaat ctttcatacc attcgtcaaa ctcatactct
     1081 gacttttcgt ccttattttc cccatagtca ttacgaacta gaggtgaggt aatattttta
     1141 tttagatcaa tgtttctttt ttgttcccta gcaaaattaa ttgttttatg catttccgct
     1201 agttcgttca tttctatatt atttttagat ttcgtcaaga atttttgaac acgttccccg
     1261 cctttataaa taggaatttc ggctttccca cgttttactt gtaaatacca tttacgaaat
     1321 gaattgtagt ctagttgttc ctctacaacc catttcggaa cacctttcat tcgtaatact
     1381 tcctcatatt caccatcggt attaatagct ttttccgcat acatttttgg ttggaaatag
     1441 agtccttcaa taacataccc ttctaacttc cactttccat actctttatc gtgaaccatt
     1501 ttatcaggaa atttcgtagt tgttgcacaa ctgtccgtat cacaataagc caaaattcct
     1561 ttttcatgcg catacttcaa tcctttaaat agtagaattc ttgcaataga agtaatatat
     1621 gctgaaatat gtggttgaat atattcagcc atagcatatc cgtcatactc taaaaattcc
     1681 attcgaattc cattcatatc atatatgatc tcgcttactg tatgtccttc tgcttcaagt
     1741 ttatctctct cagaaatatc agcatacatg attcgttccc gctgcattgc aaattttcca
     1801 tacaacgcat tctgcataag ttttgaaaag gctcgtttag cacctttact tgtatttttt
     1861 atttcctcaa aatacgaaat aaattcacga aagacaggtg ctgttttctc aaatacaaca
     1921 ccgctttcga ttttttctat tttgcaacct tccgcttctg ctaaagctag ttccggaaat
     1981 gtccaaacac cttctatttt accaactggg aaaatcaatt tacctgtata gtctttttta
     2041 ggtaagatag gaatatacat atcttcgggt acatgtacct tcgcatgtat aaagcctgct
     2101 ccatatcttc ttcttttcca tagatcaaaa gaaagttctg cttcttcatt atccagtaca
     2161 ttcgggtatc cgactggcat ttcagccatt ttcataacat atggatataa actattttta
     2221 tcatagtggt atccgttttc tattcttggt gtaaaaactt cggttcttcc tccatagtag
     2281 ccttttctaa taaatgcctc taaacctttt cctagttgtt tatgattata ttgttttgta
     2341 gaaatagcaa ctttataatc cttcttatac tgttctttat aaactgtttt agctagggaa
     2401 gctgtagtcg gacaattaat aaattgttca acttctaatt ttgataggcc tattacaatt
     2461 tccaagattt tatataaact acgacaatca tattccatgt actcacatag gacagggtca
     2521 tctttatcta ctgttgtaaa gaaattacct tttgtaagtc gcttgttcaa tttacggttt
     2581 tttacattat aaataccgta attattttct tccatataat cgacaatatc catcttccca
     2641 ccttctaaaa ggtcgaaatc tctacataag ttttcaagtg aagacggtag caatcggaag
     2701 ctatcatgta gtataatatg agaagcggta aacgttacta tattaccatt gataaatagc
     2761 gagttattaa atgttggttc agcataatca cgtaactctg ctatgatctt ggaaaggtcg
     2821 aagtctaggt tatgtatata tacatgacag tcatatgctt tgtacttttc aaacactggt
     2881 aatacgtctg caaacgtata gccagtatag tattgttttc catcgaatag tccaattcga
     2941 aacacgtcgc cgtccaaacc acgtgtttca gtatctagcg taaagagttt aatctctcta
     3001 cgcttttttc tattagtagt actcatcgtt atcccaactt tctttcgtat atgatttagt
     3061 atcatatatc ttctgaatct cattagcgtt cctatgtgct tgtttatatg gtaaggaacg
     3121 aagattattt aataactgct ttaaatactt gtcacggtct tttacgtcgt aaacaccaac
     3181 cattatcgtt tctattgctg ttaatagttt tgtaagttct tttccttgtc cctcatatat
     3241 ctttacatag ccgtgattct tccaaagtgt attggtttta gatacatcac gttttgggac
     3301 aatccgcata gcaacccttc ctaaaaatag tttgttctgg gcgttctgca accaacctat
     3361 tatagaccta gtaatttggc tacgtgatct tgtattgttt ctctttttaa aatcatatat
     3421 aaacttactg tctgtctcac ctgtaaagtc ctgatagtat attactagaa aaggtgcttc
     3481 cctatcttgt tcttttttta ttctctctat ttccggaagt aactttttcc atgaattaaa
     3541 cttactcggt gaaagtcccc actgtcttgc tagatttgct atttccctag cacgctctct
     3601 atttttccta ttatgcgctt tctttttata aacagtttct aactctctac ctttcagctt
     3661 ctttgtttct ttcttactat aaccttctga aacaagtaac gactttttac gtttcgtttc
     3721 taatttcttc tttaaccgtt tatttgccat tgtatcacct acttataaag ccatgtgacc
     3781 cgtaacaact aggtcacagg tctttggctt acatggcttt cttttctgta atagagttca
     3841 taaagttggc tatcacatta tcaaaattag acatagctgc aaccatgtct aactcttctt
     3901 ttgttaacac gacggatgtt tcatcttctt ctaacttttg cttcaaatgc accattccat
     3961 taaaagagac aaattccagt tctcgttcat ttgtccgaat agatacttga actaagtttt
     4021 taatagcgtt cacgaccgca ccatcctttt atgttttaaa aaagaggata cacaatgtgc
     4081 accctcattc atttgttaca aaaattagcc ttgtagttga agagttaatg ttttaaatcc
     4141 tttacgtgtt ttcacttctg ccggaacaat ttctaaaggc tcgttccaag gtgctggacc
     4201 tacaatacta ataatctttt gaatagatga tacgacccct tgtgacaccg cgtggtatgc
     4261 tttaccgttc ttatcaatta aaacaacacg taatgcatcc acatcttctt ttgtcaattc
     4321 atcttgtaga gtaatagcat gagcaaccat gtccgtgatt tctaatactt cacctctatg
     4381 atctgctaga gcattctcac tagagttaat agcattataa actttgatag ctgatttacg
     4441 atctttactt tgaatagatg agaagaatac atccttcgct ccgtccgctt caaagattga
     4501 aggtgacacc ttgtccactg ttacgatgtc atttgtattt acatgctcgt ccactaccgc
     4561 gatttcgtta acctcattat ttaattgttt agtattgttt tccattgaat attcctccta
     4621 ttaattgtcc attttatttt tattttccaa tagtcctttt gcatcttcct ctttaataag
     4681 gaatttgttt gctaccttag aagcgtctaa ctttccagaa tgtatcattc tatggatagt
     4741 ctttttatga acacgcaata agtctgcaac ctcttgcgca gtcagtagtt tactcatatt
     4801 acatatgcac caccttcttt tcacttgtat agagagtatg tatcgtgtct cgatttgtcc
     4861 cgtttgtccc tctctgctac caatattacc cgaaagttac cagtaatgca aatgttttta
     4921 tgttacagtt atgttacaaa aaccgaacat ctataaaacg cgtgttcgtt ttcaactcat
     4981 ttcctattat agcacccttg gaatatcttg tcacactttt ccttttggaa attccaataa
     5041 attttccaaa ttgtcaatag ttaattttag aaaattttgt atatcatatt tcataaatgt
     5101 tattacgtgt caataggtaa
//
