LOCUS       NZ_CP083112             8279 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain ABTS-351 plasmid pCH_183-k, complete
            sequence.
ACCESSION   NZ_CP083112
VERSION     NZ_CP083112.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214338
            Assembly: GCF_020809105.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x, 70x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809105.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,043
            CDSs (total)                      :: 6,891
            Genes (coding)                    :: 6,549
            CDSs (with protein)               :: 6,549
            Genes (RNA)                       :: 152
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 116 of 342
            Pseudo Genes (incomplete)         :: 252 of 342
            Pseudo Genes (internal stop)      :: 75 of 342
            Pseudo Genes (multiple problems)  :: 77 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083112.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="ABTS-351"
                     /isolation_source="Biopesticide DiPel DF"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_183-k"
                     /collection_date="2017"
     gene            join(7891..8279,1..1984)
                     /locus_tag="K8Z42_RS34875"
                     /old_locus_tag="K8Z42_34830"
     CDS             join(7891..8279,1..1984)
                     /locus_tag="K8Z42_RS34875"
                     /old_locus_tag="K8Z42_34830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_001162327.1"
                     /translation="MALDIAKCGRRTIVLREKATGDVMIRRMSCNQLSCPVCGPVIAAR
                     LQLRIKYFSQHERLFFFNTITSKDGIDDLEKTFKKIRKELSSDFTIEGYMRRKKVSYEK
                     ALKWYEKKKKKMIDFDIDVELGIYSRKNAIIEVANLKKVYYLGLNVKQKIEFHKKYNHL
                     VDELFPAIHEVNKRDEILHKQLYERILRRFEENEFEDFKFIRVVELHESGQPHYHFLCN
                     RYVSHYLMKKITVEGVSEVYDNAYIVDHAMERNKTLTYDTVDTDIVSFYVSKVTRYVTK
                     ETIETFSELEKEGDIAKKLISSSNCIKLFDEKDEEESKYKKLGVYDYQINATSYEVPKV
                     LENSVEDFIKFHSVMLRDQYAIDLLKFSKSLDLDDFINCNYVIHERLIQVSVAEYKNKL
                     STVFDETDLTKEQLELLKSFVENKITLLLGRAGTGKTYTLVNLLKKLKPNPRTTFILTY
                     TGKASVRIKELLSQHELTEYKPMTVHKACSSNWNSNFLKNEYNNLECEYLIIDEVGMIP
                     RDILMKVLLAVPSYTKIIFAGDDAQLPPVNDSSVIPELEGLDFINTIRLTKVFRSGDTV
                     LELAYKVLNRELIDFRLYDEKQLEKLVLPLVEQGYQILTNTKKMTKKINLIVQANKDKI
                     TKCFNDYCYEIGDRVMIIANDSSRRVYNGDTGKIVDYDDKGILIQLDFEDRQVFYRYLD
                     TVNIVPAYAFTVHKSQGSEYENVAVFLESQPKLNTNNLLYTGITRAKKNIKVYVPDEKV
                     LSTMINCVPEPVSNITLNNVFDLVNGILDIKKTSSMG"
                     /name="AAA family ATPase"
     gene            2123..2320
                     /locus_tag="K8Z42_RS34880"
                     /old_locus_tag="K8Z42_34835"
     CDS             2123..2320
                     /locus_tag="K8Z42_RS34880"
                     /old_locus_tag="K8Z42_34835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000274139.1"
                     /translation="MYGAYLDPEDRDRIMGSLSDEQQEYLRIFLKRGNQTAFSSVLARL
                     KGGESEEDAEHISTQWMFIE"
                     /name="hypothetical protein"
     gene            2492..3142
                     /locus_tag="K8Z42_RS34885"
                     /old_locus_tag="K8Z42_34840"
     CDS             2492..3142
                     /locus_tag="K8Z42_RS34885"
                     /old_locus_tag="K8Z42_34840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3895 domain-containing protein"
                     /protein_id="WP_000276556.1"
                     /translation="MYKIDCELDEILIKISSGWSLFENLGMDSIPNDLIMPIDIREHLE
                     LDLPLLERQVMRLKGDLRSYNSLKKNIVEKNDSVDFEINEEREEGQASLFDEDIVFGFN
                     LDDDNVQTIRSQPVKVKPVSIEKNISYQFLSFVEQEFILQFIQNVNSVSVRQLCEFMIK
                     EVGSLDKRYLTGKPHIYVHVCSYLDFLVTQGKLSYIENLDYIDRIYYIESDIS"
                     /name="DUF3895 domain-containing protein"
     gene            complement(3174..3314)
                     /locus_tag="K8Z42_RS34890"
                     /old_locus_tag="K8Z42_34845"
     CDS             complement(3174..3314)
                     /locus_tag="K8Z42_RS34890"
                     /old_locus_tag="K8Z42_34845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000130115.1"
                     /translation="MTDKKERVEMRIPQSILKKVDEYKEENGISTRTATILELIRKGLN
                     K"
                     /name="hypothetical protein"
     gene            3425..3676
                     /locus_tag="K8Z42_RS34895"
                     /old_locus_tag="K8Z42_34850"
     CDS             3425..3676
                     /locus_tag="K8Z42_RS34895"
                     /old_locus_tag="K8Z42_34850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000888627.1"
                     /translation="MLEDKFLESKIKKLTNQNVVVFTDDEIIEGVLLDKDTGEERLFEV
                     FRKIWGKNFAWFGEGIYIQCEDTVTFRDYDEVDNIFKK"
                     /name="hypothetical protein"
     gene            5827..6348
                     /locus_tag="K8Z42_RS34900"
                     /old_locus_tag="K8Z42_34855"
     CDS             5827..6348
                     /locus_tag="K8Z42_RS34900"
                     /old_locus_tag="K8Z42_34855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_000234812.1"
                     /translation="MVIIYQTTKQAAQTLNINHTTFKKYYGMFERYTGYNFLRDSKGQV
                     MFSEYDLEVFKRLLLVKAEPGRTIEESCRLVGEEFGISDKNRVITDISPENGGDNKAIE
                     ELKELILMQNNKIDELTIKLNEQSNQTKMIETSVGDRDQQLVRLMKEMLEVKRMVAASN
                     KKRWWHFFRK"
                     /name="DUF3967 domain-containing protein"
     gene            6433..6717
                     /locus_tag="K8Z42_RS34905"
                     /old_locus_tag="K8Z42_34860"
     CDS             6433..6717
                     /locus_tag="K8Z42_RS34905"
                     /old_locus_tag="K8Z42_34860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391901.1"
                     /translation="MEELNKKPQAEMVRVNTRISKEVNGWLDEKSARTGVPKSTLIYLA
                     LEQYIQQQTMFEELPKLQQMYTQIQQQQMVQGDLLSVVNKDNNLAKFSL"
                     /name="hypothetical protein"
     gene            6743..6985
                     /locus_tag="K8Z42_RS34910"
                     /old_locus_tag="K8Z42_34865"
     CDS             6743..6985
                     /locus_tag="K8Z42_RS34910"
                     /old_locus_tag="K8Z42_34865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000340717.1"
                     /translation="MDDALTKILEKLDQLDKKVDTIGSEVKEIRKEMQAHRMEIDDNFT
                     KLYKLVEDKDVEIDVLNKRVFKNETTVEKLKRQKH"
                     /name="hypothetical protein"
     gene            7056..7409
                     /locus_tag="K8Z42_RS34915"
                     /old_locus_tag="K8Z42_34870"
     CDS             7056..7409
                     /locus_tag="K8Z42_RS34915"
                     /old_locus_tag="K8Z42_34870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001179589.1"
                     /translation="MQNIKEIKKLIRKNPGKYASISTTEDGDFEGVLLSGFFAKQVMKL
                     ASQSNHISHDFNSNDLRSSDLTLKGVFLFNGTGVHFVFYEGLESIRIYDSPMFEEDSKE
                     MFEYFKEWLHAHS"
                     /name="hypothetical protein"
     gene            7549..7797
                     /locus_tag="K8Z42_RS34920"
                     /old_locus_tag="K8Z42_34875"
     CDS             7549..7797
                     /locus_tag="K8Z42_RS34920"
                     /old_locus_tag="K8Z42_34875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000364675.1"
                     /translation="MDLKTYYANVINELESILLSSEKVCEDNFLKIEQYLSNKSFIEND
                     IDYIFYHLDNLECLVKDRVFLEDALKRYREKLDKVKK"
                     /name="hypothetical protein"
     Typing          6434..6717
                     /name="rep_cluster_2006"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.17e-147"
ORIGIN
        1 aaaaaatgca atcattgaag ttgcaaattt aaaaaaggtt tattatttgg gtttaaatgt
       61 taaacaaaaa attgaatttc ataagaaata taatcattta gtagacgagc tatttccggc
      121 tattcatgaa gttaataagc gagatgaaat attacataaa cagttgtacg aacgaatttt
      181 aagacgtttt gaagagaatg aatttgaaga ttttaagttt attagggtag ttgagttgca
      241 tgaaagtgga caacctcatt atcatttttt atgtaatcgt tatgtttctc attatttaat
      301 gaagaagata acagttgaag gtgtgagtga ggtttatgat aatgcgtata ttgtggatca
      361 tgctatggag agaaataaaa ctcttacata tgacactgta gatactgata ttgtttcttt
      421 ttatgtatcg aaagttacta gatatgttac taaggagact atagaaacct tttctgaatt
      481 ggaaaaggaa ggtgatatag ctaagaaatt aattagttca agtaattgta tcaagttgtt
      541 tgatgaaaaa gatgaagaag aatctaagta taagaaatta ggggtatatg attatcaaat
      601 taatgcaaca agttatgagg ttccaaaagt attagaaaat agtgtagaag attttattaa
      661 gtttcattct gtaatgctta gggatcagta tgcaattgat ttattgaaat tttcaaagtc
      721 attggattta gatgatttta taaattgtaa ctatgtaatt catgagcgtt taattcaagt
      781 ttcagttgct gaatataaaa ataagttatc gactgttttt gatgagactg atttaactaa
      841 agaacaatta gagttattaa aatcttttgt agaaaataaa attacattat tgttaggacg
      901 agctggtaca ggtaaaactt atactttagt taatttgttg aaaaaattaa agcctaatcc
      961 acgtacaact tttattttaa cgtatacagg taaagcgtct gtacgaatta aagaattgtt
     1021 gagtcaacat gaattaaccg aatacaagcc tatgacagtt cataaagcat gttcatctaa
     1081 ttggaattcg aattttttaa agaatgagta taataactta gaatgtgagt atttgattat
     1141 tgatgaagta ggtatgatac caagagatat tttgatgaaa gtattattag ctgttcctag
     1201 ttatacaaag attatttttg ctggtgatga tgctcaactt ccacctgtta atgattcttc
     1261 tgtaattcct gaattagaag gtttagattt tataaatact ataagactta caaaagtttt
     1321 tagaagtgga gatactgtgt tagagcttgc atataaagtt ttaaatcgtg aattaattga
     1381 ttttagatta tatgatgaaa aacaattaga aaaactagtt ttaccacttg tggaacaggg
     1441 gtatcaaatt ttaacaaata caaaaaaaat gactaagaaa attaatttaa tagttcaagc
     1501 taataaggat aaaattacga agtgttttaa tgattattgt tatgaaatcg gtgatagagt
     1561 catgatcatt gctaatgatt catctagaag ggtttataat ggtgatactg gtaaaattgt
     1621 tgattatgat gataaaggta ttttaattca attggatttt gaagataggc aagtgtttta
     1681 tagatattta gatacagtta atattgtacc agcttatgca tttacagttc ataaatctca
     1741 aggtagtgaa tatgagaatg tggcggtatt tttagaaagt cagccgaaat taaatactaa
     1801 taatttatta tatacaggaa ttacaagagc gaagaaaaat ataaaagttt atgtgccgga
     1861 tgaaaaggtt ttatcgacta tgataaattg tgttccagaa ccggtttcga atataacatt
     1921 aaataatgtt tttgatttag taaatgggat tttagatata aaaaaaacat cttcaatggg
     1981 ctgaagatgt acaaaaatgt agattcttca atgggttgaa gaatcataag actgaaatga
     2041 ttgttaaaga atctaattgt attatatatg taatttgtag catggtctat ttaatttaat
     2101 ttttcggaag gatttgttgt ttatgtatgg tgcttattta gatccagaag atagggatag
     2161 aataatgggt agtttgtctg atgaacaaca agagtattta cgtatttttt tgaagagagg
     2221 taatcagaca gcattctcta gtgtgttagc tagattaaaa ggtggcgaat ctgaagagga
     2281 tgctgaacat atatctactc aatggatgtt tattgagtag attgatgcag gaaaagtttc
     2341 gaaagatcat ccgtgtgagt gtggtcgtcc tttacgttat caatatattg ttaagaattt
     2401 agtgactaat aaaattttaa agtttagtaa ggatcacttt aaaaaacata caggtttacc
     2461 ttctagtatt gttaaggcag ttttgaaggg aatgtataag attgattgtg agttagatga
     2521 aattttgatt aaaatttcat ctggttggtc tttatttgag aacttaggta tggattctat
     2581 tcctaatgat ttgattatgc ctatagatat tagagagcat ttagaattag atttaccttt
     2641 attagaacgt caggttatga gattaaaagg tgatttgaga agttataatt cattaaagaa
     2701 gaatattgtt gagaaaaatg attctgtaga ttttgaaata aacgaggaaa gagaagaagg
     2761 acaagcttct ttgtttgatg aagacattgt ttttggattt aatttagatg atgataatgt
     2821 tcaaactata cgaagtcagc ctgtgaaagt gaaacctgtt tctattgaga agaacatttc
     2881 gtatcaattt ttaagttttg ttgaacaaga atttattttg cagtttatac aaaatgttaa
     2941 ttctgtaagt gtgagacaac tatgtgaatt tatgattaaa gaggttggct ctttagataa
     3001 acgttatctt actggaaagc ctcatatcta cgttcatgtt tgtagttatt tagatttttt
     3061 agtcactcaa ggcaagttga gctatattga gaatttagat tatattgatc gtatttatta
     3121 tatagaatca gatattagtt agaagggata tgtatattat cccttctatt tttttattta
     3181 tttaatcctt ttcttataag ttctagaatt gttgctgttc tagtagaaat tccgttttct
     3241 tctttgtatt cgtcaacttt ttttaatatt gattgtggga ttctcatttc tactctttct
     3301 tttttatctg tcattattat cacctcttct tgtattgtac ggaaaaatat cgtataatac
     3361 aaattgtacg gaaaaaagac gtatttttca cgtacaaaaa atgaaaatca aaggggattt
     3421 tattatgtta gaagacaaat ttttagaatc taagattaaa aaattaacta atcaaaatgt
     3481 agtggttttt actgatgatg agattattga aggagtcctt ttggataagg atactggaga
     3541 agaacgtttg tttgaagttt ttcgtaagat ttggggtaag aatttcgctt ggtttggaga
     3601 aggaatttat attcaatgtg aagatacagt gacgtttcgt gattatgatg aggtagataa
     3661 tatatttaag aaatagtttt atataaattt atattaaatg cagattattt atagaagagc
     3721 ataaggtttt tttgctatca ctaaaggtga caggttagac aaagcctaac aaaaacgtga
     3781 tatcataaat atgagaaaat caatggagtt tcaatgtgtt tccaatgggt tttcactgta
     3841 tataatatgt tattagaaat tggttttttt gcttttagat tgataattct tgtgaaagat
     3901 aaaatgaaat tccatttata aatgttgtat attttaatag ttttatagga gtttttaaga
     3961 agaggttgat gaggatcaat gcatagcagc catgtgcatt attgtattag cgaataaaaa
     4021 aaacgatatg ttaacgttac ccaaaaggat agctttaaca tatcgttttt tttattttta
     4081 tgcctactac ggtaaggcta caccaatttt ttagttccaa ctaaggtatg gatactccaa
     4141 aattaatcat cattcttttg ttttatgttg ttgcgaaggt gaacagaaaa aaacttcgct
     4201 gaatttcgtt tttattttac cacaaattta attttttggt gcatttttag tgaccactga
     4261 gtgaccacta agtgccaaca aatgaccatc agaatgaaaa aatgaccacc cagtgaccac
     4321 caattaataa ataggcgtcc cagttttcgt ggtcgtttag tggtcatttt tggcgcgtac
     4381 aaaacgcata cttttaattt ttttcacata caaaacgcat ataaaatgac caaaaatgca
     4441 tacagaacgc atatgagtaa aaaaagacgt cccacttttt cgggttaaaa aaacgttgat
     4501 ttaaaggggc gtaaagcgtg ctacaaggga cacaaaacgt gccacaaaag tgctttaaac
     4561 gtacacaaaa cgggctacaa agcccaaaaa ggcatcccgt aattcacaaa atagaaaccc
     4621 aataggctca gcctatttca tcactttttt tataaaatgg gattcggttt ccttatgctc
     4681 ttctttaaaa aaaggtttat atggcttttt aagctggtaa atattttgta ttaaaaaaag
     4741 aaaatgcagg ataaggagag ggacacagtt gtgtctacgt gtatcagtca aagcctgata
     4801 gaataatttt tcattgtgta cagagttgtg accacgattg aaaaagggaa tgcgttttgt
     4861 ggtcacaaaa catttataag gtctatttta ctaatgtttt gatgtagcct tactaataga
     4921 tccgacagat taaataatta atgtttgtgg caattagcga ggaatggcga agattaggga
     4981 agacattttt tgaagtatat tggggcgaag tattaagatt tttatccaag taatcgttat
     5041 aaaaaaatca aaatgaaatg gcagcaggac taaattcagg atcgttggag gtttaaatac
     5101 gaatatagtg ttggggtctc aagaagagat aagtatatat ttagagattc tatagaaaaa
     5161 atatttttaa tatttgtcgc agaattagtg cttacgccct aaagattatg ttaggtatgg
     5221 cgcgcattcg gccttaggcg gatgcgcgcc agggatagtt aagcgttaag aggatgaaaa
     5281 atattaacaa ggtagttatc tatagagtta tttaatatat agttggatct aattgtgagt
     5341 tatagaagat atagccgata taacttcagc tataaaggta gaatgattaa aagaaggaac
     5401 gatgtatata ttcatcagaa tacgcaaaat cacttctgga aggtttgaaa tgtgaatcgg
     5461 tagatggaaa tggaaagcct gtaagaagcc gtagcaaggc gaaggagttt tatttagaaa
     5521 agttttttga tttgtaaaaa gttttttaaa taaaacttga tgtcccgtag ggttgcctaa
     5581 aggaatagta aatcaagcta ttgcgtactc ccaaacattg tttgggggac actcgctgtg
     5641 agcgtaaagg gtgtgggaat atcccacgaa taaaaaattt ttgtgtccct ttttttaaaa
     5701 aggacggttt gattttgtgg aataatacgg gacgtgaaat tttattatga tttataaagt
     5761 ttatataaat tataaaacaa ggagttatat cggttatatc gaatgtaaat ttgtgaggtg
     5821 attgcaatgg ttattatata tcaaacaaca aaacaagcag cacaaacatt aaatattaat
     5881 catacaactt ttaaaaaata ttatggcatg tttgagagat ataccggata taacttctta
     5941 agagattcaa aaggacaggt aatgtttagt gaatatgatt tagaagtatt taagaggtta
     6001 ttattagtta aggcggaacc aggaaggact atagaagaat cgtgtagact tgttggtgaa
     6061 gagtttggta tttcggataa gaatcgagtt ataaccgata tatctcctga aaacggaggg
     6121 gataataagg cgattgaaga gttaaaggaa ttgattttaa tgcagaataa taaaattgat
     6181 gaattaacta ttaaattaaa tgaacagtca aatcaaacta agatgattga aacaagcgtt
     6241 ggagatagag atcagcaatt ggttagatta atgaaagaaa tgctagaagt gaaaagaatg
     6301 gtggcggcaa gtaataaaaa aagatggtgg cattttttca gaaaataaat ttagccgaaa
     6361 aggctttttt tattgtttta tagactctat tgatgtaaaa ttggtgttaa taaaacatca
     6421 aggaggatca agatggaaga attaaataaa aaaccacaag ctgaaatggt tcgtgttaat
     6481 actcgtattt ctaaagaagt taatggttgg ttagatgaaa aaagcgctag aactggtgta
     6541 cctaaatcta ctttaattta tttggctttg gaacaatata tacaacaaca aacaatgttt
     6601 gaggagttac ctaaacttca acaaatgtat acgcaaatac aacaacaaca aatggttcaa
     6661 ggagatttgt taagtgtggt taataaggat aacaatttag ctaagtttag tttgtaattt
     6721 aattttagga ggagaattaa atatggatga tgcattaaca aaaattttag agaaattgga
     6781 tcaacttgat aagaaagtag atacgattgg tagtgaggta aaagaaatta gaaaagaaat
     6841 gcaagcacat cgaatggaaa tagatgataa ttttacgaag ttatataagc tagttgagga
     6901 taaagatgtt gagattgatg ttctgaataa acgtgtattt aaaaatgaaa caacagttga
     6961 aaaattaaag agacaaaaac attaggattc atatgtgaat taaaattata tgtgtttatt
     7021 tttaaagaaa gaagatattt aaggggaatg atatcttgca aaatattaaa gagataaaga
     7081 aattaattag gaagaatcct ggtaaatatg cgagcatttc tacaactgaa gatggtgatt
     7141 ttgaaggggt tttattgagt ggattttttg caaagcaagt tatgaaattg gcttctcaat
     7201 ccaatcatat ttctcatgat tttaattcaa atgatcttag atcttctgat ctaactttaa
     7261 aaggagtttt tctttttaat ggtactggag ttcattttgt tttttatgag ggtcttgagt
     7321 ctataaggat ttatgattcg ccaatgtttg aagaggattc taaagagatg tttgagtatt
     7381 ttaaggagtg gttacatgca cattcttgat atttacttta gtaaacgttg tgagaatgta
     7441 ataaaacata tttaacagtt tactgtgtaa atgggcactt tgtggaattg tttatagaga
     7501 ggtgttgtta tggacacttt gtggaattgt ttatagagag gtgttgttat ggatttaaaa
     7561 acgtattatg cgaatgttat taatgaacta gaaagtattt tattgtcttc agaaaaggtt
     7621 tgtgaagaca attttttaaa gattgaacaa tatttaagta ataaatcttt tattgaaaat
     7681 gatattgact atatttttta tcatttagat aatcttgagt gtttagtaaa agatcgtgtt
     7741 tttcttgaag atgctttaaa aagatataga gaaaagttag ataaagtaaa gaagtaaatt
     7801 catagtttaa tgtagtttct acttaactag ttaataataa tagggataga gtcgcacttt
     7861 ttattcgttt tcttagaaag gttgtttttt atggctttag atattgctaa atgcggtcgg
     7921 cggaccatcg ttttacgcga aaaagctact ggtgatgtta tgatccgaag aatgtcctgt
     7981 aaccagcttt cttgccctgt atgtggtccg gtcattgcag cacgtttaca acttcgaatt
     8041 aaatattttt ctcagcatga acgtctattc ttttttaata ctattacttc taaggatggt
     8101 attgatgatc tggagaagac ttttaaaaag attcgtaaag aattgtctag tgattttacg
     8161 attgaaggat atatgagacg taaaaaagtt agttacgaga aagctttaaa atggtatgaa
     8221 aagaaaaaga aaaaaatgat agattttgat attgatgttg aacttggaat ttattcaag
//
