LOCUS       NZ_CP083111             8513 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain ABTS-351 plasmid pCH_183-j, complete
            sequence.
ACCESSION   NZ_CP083111
VERSION     NZ_CP083111.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214338
            Assembly: GCF_020809105.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x, 70x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809105.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,043
            CDSs (total)                      :: 6,891
            Genes (coding)                    :: 6,549
            CDSs (with protein)               :: 6,549
            Genes (RNA)                       :: 152
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 116 of 342
            Pseudo Genes (incomplete)         :: 252 of 342
            Pseudo Genes (internal stop)      :: 75 of 342
            Pseudo Genes (multiple problems)  :: 77 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083111.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="ABTS-351"
                     /isolation_source="Biopesticide DiPel DF"
                     /db_xref="taxon:1428"
                     /plasmid="pCH_183-j"
                     /collection_date="2017"
     gene            join(8318..8513,1..2)
                     /locus_tag="K8Z42_RS34830"
                     /old_locus_tag="K8Z42_34785"
     CDS             join(8318..8513,1..2)
                     /locus_tag="K8Z42_RS34830"
                     /old_locus_tag="K8Z42_34785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000361625.1"
                     /translation="MDKSSILKQMYFILFPVTLFLSLNIYQWIVKSDYSFTGYIIQNLI
                     IVALALVAAIIAYLITKKNI"
                     /name="hypothetical protein"
     gene            1375..2580
                     /gene="mobV"
                     /locus_tag="K8Z42_RS34835"
                     /old_locus_tag="K8Z42_34790"
     CDS             1375..2580
                     /gene="mobV"
                     /locus_tag="K8Z42_RS34835"
                     /old_locus_tag="K8Z42_34790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033305.1"
                     /translation="MSKFVICHMQKFKMRDVKGLQIHNQREKESHSNSDIIQARTEQNY
                     DLIHDKEKVDYKKLIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQVEQRRF
                     FEESLRFLKGRYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSEFHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEELEQEKKGIDSRLHDLKM
                     SLDQAKSVDEIPVKEKGGFIRSKTVEITSEDFESIKVLARSSEALREDVRRLKNESGVI
                     TREKDGLYREKCILEGQITELKRENRGLKEENDFFKKTLERVKELYKEKLPELAGVIGY
                     VKGSILDRMNCKFLKRYFTSDDEVRGAQKFLNHKQEHEEQQKRLKQVRSSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            3050..4096
                     /locus_tag="K8Z42_RS34840"
                     /old_locus_tag="K8Z42_34795"
     CDS             3050..4096
                     /locus_tag="K8Z42_RS34840"
                     /old_locus_tag="K8Z42_34795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001085408.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIKKKMSESGKELFKSCSIF
                     NEFLATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQEVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELSESEKQEYNGHL
                     INEVDVEA"
                     /name="protein rep"
     gene            4100..4297
                     /locus_tag="K8Z42_RS34845"
                     /old_locus_tag="K8Z42_34800"
     CDS             4100..4297
                     /locus_tag="K8Z42_RS34845"
                     /old_locus_tag="K8Z42_34800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861196.1"
                     /translation="MKVAGDLYYYCLGCKKFHEYEKIDHKGVNRKLCFYCFKIQSKKTK
                     IIGDAEGRMQICETCHKELF"
                     /name="hypothetical protein"
     gene            complement(5166..5429)
                     /locus_tag="K8Z42_RS34850"
                     /old_locus_tag="K8Z42_34805"
     CDS             complement(5166..5429)
                     /locus_tag="K8Z42_RS34850"
                     /old_locus_tag="K8Z42_34805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874967.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISINDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            5703..5930
                     /locus_tag="K8Z42_RS34855"
                     /old_locus_tag="K8Z42_34810"
     CDS             5703..5930
                     /locus_tag="K8Z42_RS34855"
                     /old_locus_tag="K8Z42_34810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869869.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869869.1"
                     /translation="MKVVSNIRMIMAKKNIDNISDLVRITGVSRNSINKLWHNENVSSL
                     KLDTLITICEKLDVELLDLIEYIRDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            6402..7481
                     /locus_tag="K8Z42_RS34860"
                     /old_locus_tag="K8Z42_34815"
     CDS             6402..7481
                     /locus_tag="K8Z42_RS34860"
                     /old_locus_tag="K8Z42_34815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102658.1"
                     /translation="MNVQLGNEQITKLLNDWYQEIRAQHIIKAKQLKQKIEKEINNIEE
                     DQNLLLYYSLIDLRYKILTNDYADREQSLEKIEQLKEHTNNFLDYYYHFFKGMHAMKTG
                     NYSETQKYYDIAEKLLEEIPDEVEKAEFNYAVATFYYHTHQALLATQYANKAKSFFSGK
                     LGYEIKTGACENTLGMACITLREFSVAEEYLLSAINKFEKHSENKLALIVRYNLGLLYA
                     DQNLSELAIRYLLESFENQEDDYKTMFLLAQEYYKLKQTNTVNTYIEKGLNVCNQEYKI
                     HFLFLQALNNKLSTEQLEKVMLDAIPYFEKQNLWKYIQDYTEELAIRFYEEKNKDKSNE
                     YFYKSYKAKRNLLERESLK"
                     /name="RapH N-terminal domain-containing protein"
     gene            7478..7624
                     /locus_tag="K8Z42_RS34865"
                     /old_locus_tag="K8Z42_34820"
     CDS             7478..7624
                     /locus_tag="K8Z42_RS34865"
                     /old_locus_tag="K8Z42_34820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000976019.1"
                     /translation="MMKKFSLILIGVACTTGIFFSQFNNSIQTHDAKEKNDIIQQYAHG
                     KDI"
                     /name="hypothetical protein"
     gene            7744..8325
                     /locus_tag="K8Z42_RS34870"
                     /old_locus_tag="K8Z42_34825"
     CDS             7744..8325
                     /locus_tag="K8Z42_RS34870"
                     /old_locus_tag="K8Z42_34825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000723966.1"
                     /translation="MKKISIERSNWRKTYKKMIPVLLVGGILAGNVAFVTNNVFAATTN
                     NNNIIQTKQSLVSGQDEKSINELAEAFQFMHEEAAIYNNQGEIVDFDFNKIEAKYGNDV
                     GLDALKQQIKKDKSDIAGLSFTSCMKDALYDFFGVNAVQAAFNGGLAYYLEKKLWKEAA
                     KIGVKFFIGSSVAGLAATLAYYGGKCAIYG"
                     /name="hypothetical protein"
     Typing          3026..4078
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.934"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          1376..2577
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacacataat gttagtctaa ataacagaaa attctaaccc ttattcttat tttgtattat
       61 actggttatg tacaggtaaa atggtttaca agttaatttt tcctcgtttc aaaaaaagtc
      121 cattcttttc aaaagaatgg acttttttct tgttatattt gctgtaacta ttaaaagggc
      181 atcaaaacga tctacaaaag cctcaagacg acccacaaac catctttaaa cggtctcaaa
      241 acgaactaca acccgaaaaa acgcatccca gtttttttag gtttaatttt aagcgcatac
      301 caagcgcata taggcgcata cgggacacat acaaagtgcc gaaaaacgca tataggtcgc
      361 atacaaacgg tagaaataca tccctaaaaa tagagtgata gaaacccaat aggcacagcc
      421 tatttcacca ctttttttgc aaagtgggat tcggtttcct tatgctcttt ttttattttt
      481 gctttttaag ggtcttataa tcgtttttaa gtattttagg atgttgggtt tcttaaggga
      541 actagaagtt cctagaatgg attctgagcg ttctgaggtg tatttttcta tggattcgat
      601 attggaattg ctctaggaat agttgtcgtt ttattctgtg gctgttccga cagtggttct
      661 cataaagaat atgattgtat caatagattt gatgatgttt gaaaaataga gaatagttga
      721 agctcttcct gtaaaacaaa aaaaattatg tttgtagagg aagtagtcat ttcactacaa
      781 gaaaattccc atagatcggt ggcgagttga aaaatgttga aggttggcga ggacatttta
      841 cgaggacgcg ggaatggctt tgtcattgtt ggggttaata gtttttaaga aaagttggtt
      901 agtagctgaa aaaaataggg aagattggcg aggatgtatt gcatggcagc gggacatgtt
      961 atgtagtcgt ggtgaatata aaaagagact ttttaataca tggatgtcaa ctgtaaaaag
     1021 ttagaggtag ttgtatgatg tttgtccaac ggtaagcgga acgaagtgag cattaaggag
     1081 tggacgaacc cactgtgccc agaaccctct tggaactcca aaacgttagc cgttttgggg
     1141 atgccaaccg gcgagggagt ccctcaagga ttgaggggtt ggggagttcg attgaaaggg
     1201 gtcaagggga agagtttccc ttgtgggttt gggcaaagcc catgattttt tttgccatgc
     1261 ttgttatggc tcggcggtag ccgaaatgtc ccgaagggca cacgtgttta cgcagtaaac
     1321 tgtagtgtgc tactgtttac atggaactac ccccgaaaaa aaggagcgag gcccatgtcg
     1381 aagttcgtga tttgccatat gcaaaaattt aagatgagag atgtgaaagg attacaaata
     1441 cataatcaac gtgaaaaaga aagtcattcc aattcagata ttattcaagc acgaacggaa
     1501 cagaattatg atctgattca tgacaaagaa aaagtggatt ataagaaatt aattcaaaat
     1561 cgaattgata atggtgttgt atctaatcgt gcaattcgga aagacgctgt tgtttgttgt
     1621 tcgttcatga tttcggctag tccggattat atgaattctt tatctcaagt ggaacagaga
     1681 cgattctttg aagagtctct tcgttttttg aaggggagat atggagaaga gaactttgtg
     1741 tatgcttccg tacatgttga tgaaaaaaca cctcatatgc atgtgggtat ggttccagtt
     1801 aatgaaaaac aaaaactatc agcgtattca ttttttaaaa acaaatcaga gtttcatgat
     1861 ttacaagata aaatctatga acatgtgaaa gaaaagggat ttgatataga gcgtggtgtt
     1921 tctagtgata gaaaacattt atcgacgcag cggtttaaag cggtgacttt acaacaagaa
     1981 atagaagaac tagagcaaga aaagaaggga atagatagtc gtcttcatga tttaaaaatg
     2041 tctttagatc aagcaaaaag tgtggatgag atcccagtta aagaaaaagg tgggtttata
     2101 cgctctaaaa cggtcgagat tacctcggaa gacttcgaaa gtataaaagt attagctagg
     2161 tcgtcggaag cccttagaga agatgttaga cgccttaaaa acgaatcagg tgtaattaca
     2221 cgtgaaaaag atgggctgta tagagagaaa tgtattttag aggggcaaat aacagaattg
     2281 aaaagagaga atcgaggatt gaaggaagag aatgatttct ttaagaaaac gttggaacga
     2341 gtgaaagagt tgtataaaga aaagcttcct gaattggctg gggtgattgg ttacgttaag
     2401 gggagtatat tggacaggat gaattgcaaa tttttaaaac gatattttac tagtgatgat
     2461 gaagtgagag gcgctcaaaa gtttttgaat cataaacaag agcatgaaga acaacaaaag
     2521 agattaaaac aagtgcgaag tagccaacag aaaaatcggg accagggatt ggagagatag
     2581 tgtggttctg tttgagtttt ttgtttttta gatttatagc gttagcggaa tttgtttttt
     2641 ataacttgct taagatgagt atgtttctca gatgttttat ccgttaaaaa aaatggtttt
     2701 gaacgaaaaa aaatgcccct gtccacggag tggatttagg ggcatttttt tatgattttt
     2761 cgtgttcgaa aaatcgagtt ttttctttct ttaatacata ggtaactatt tcggcaacga
     2821 aattgatgcc tatgtattct tttagccctt atgtatcaag ggtttgtaaa aaaaaagact
     2881 tgctttttca ggcggataga tttatcattt ttagtgtcga attaaaaatc ataaatacca
     2941 cgagaggagc aagtccaaaa aaaggattcg tacacccttt tttatatcga atatgaaatt
     3001 cagaaattag aaattctgat gtttttcaga taggactaat atatcaaata tggcaaagaa
     3061 aaatcaagat gaaatcactg gtaaattaca acctaaaaag aaacagaata tcaagattta
     3121 cgaatttata aagaagaaaa tgagtgagag cgggaaagaa ttgtttaaat cgtgcagtat
     3181 ctttaatgaa tttttggcta caaaggataa aaagaagaag aaacgagtaa aggggaatga
     3241 ttgtaaaaat cgattttgtc cgatttgtgc ttggaggaaa gcagggaagg acgcggtgaa
     3301 aatcgcaacg atgatggaag caattaagat tgaggaaaag aaagagtttc tttttttaac
     3361 gttgacgact ccaaatatta aagctgatat ggtgaaaagt gagattgata gatttaataa
     3421 agcttttaaa aagttatttg atagacgtaa tattcaacgt tctattaagg gttatattag
     3481 aaagctagaa atgacgtatg ataaagaacg attcatcact gaagaaatgt ataacaacaa
     3541 aaagagaaaa gcatattatg attctcgtgg attgaaagta gacgatcata acccgaatta
     3601 tgatacatat aatcctcatt ttcatgtatt gctttgtgtt gataaaaatt attttaaacg
     3661 taaagagctt tatattaagc aagaagaatg gttagaaatg tggcgagaag taacagatat
     3721 gccagagatt acgcaagtac atattcaaaa agtagagtta atacgagaag gaaatgcagt
     3781 tgcggaggtt gcgaagtatt cagcgaaaga ctatgaaatg tcggttagtc aagaagtgtt
     3841 tgacgtgttc tatttagcat taaaaggtcg tcaattaatt gtttacggtg gattattaaa
     3901 agattatgcg aaaaaatatg aagatggcga attggacaag tataagagta cagacaagaa
     3961 tgaatattat tatagattaa tcgcaatgtg gaataaggat ttaatgaaat ttgaacaaga
     4021 gtatcaagag ttatctgaga gtgagaagca agagtataac ggtcatttaa ttaatgaagt
     4081 agatgtggag gcgtagtgta tgaaagtagc gggagattta tattattatt gtcttggttg
     4141 taaaaagttt catgaatatg aaaaaattga tcataaaggt gtaaatagaa aattatgttt
     4201 ttattgtttc aagattcaat ctaaaaaaac gaaaattatt ggtgatgctg aaggtagaat
     4261 gcaaatttgt gagacttgtc ataaagaatt gttttagttg gttgatttgt gaaaaaagca
     4321 aagtcaaatg ttagaaattt ttaataaggg aactgactgt tatatcaagt cagttccctt
     4381 attttattga gtctattttt gagtgcagca agtagcattc aaaagttttt ttatgattta
     4441 acaaaaagga gtttttctta aatttaaatt tcgtgaattt gtaaatttat ttttaaagtt
     4501 tatttcgaga tatattttgt tctactaatt ttgttttact aacgtacgag gtgaaaactt
     4561 aaagtgtgcg catgaatttt tttagtaata tatattacga taaaataccg cacgtttttt
     4621 gatgattttt ttgaaaatat agttttgaga aattctaaat tttcggggct gattttggat
     4681 tttttatgtg ggatttgatt ttggaatttg ttaaattgga aatctcgatt tttcgatttt
     4741 cggaattttt gttttctctt ttctttgtta ttctgtattt cgaaattaag gagtttatat
     4801 aataccttca tgacgaactg aaaacgaaaa aactttgctc gcatatttaa ttcatagata
     4861 tttcatatcg ttaaaaagtt gcaccgtttc aaacgagttg tgtgataaaa agatgtttag
     4921 attatgtttg aaattgttgt ttctgtgaat tttttatttc gcgatttctg tatattttta
     4981 atccttttta ctcttaaaat ctttttgctt ttgtgcgcaa atgaaaaaat caaaaagtca
     5041 cgcgtaactt ttattaaata tataaatata ttattaaaat acctaaccct tttatgatga
     5101 aaaaatataa atagttaaag aaaaaagact gataaaaaat atcagtcttt tttctttagt
     5161 tttatttata aagagtatct ctttatggtg ttttctactt tttctctcca gttctctata
     5221 tcgttaattg atattccgat ctttattaat tcgtttggaa tgaaactgtt atctaaaatc
     5281 cataaagtga gcctttctaa tgtgattctt tcgtttccaa ttgaataaat acgttcactt
     5341 tgtttgcaag tatatggttt tttaccaaca tatttttcta cataaaagta tctttttccg
     5401 tttgctttgt aatttgttag gaatgccaaa atgataatct cctcttagtt tttttagagt
     5461 taaaaataca ttttaaaaga ttaaatttta agttatatat ttaattttaa ctctaagggg
     5521 ttaaaatttc aattaaaaca aataaaatta aataataaaa caatatattt tatttttaag
     5581 gttaatatta aatgtgaata tataaaaaat aaccttaata ttatttaatt tatgttttat
     5641 ttgtataaaa gtatccttgt aagtaaaatg gtatgtacat tgaatgaaaa ggagtatgaa
     5701 aaatgaaggt agtaagtaat attcgtatga ttatggcgaa aaagaatata gataatattt
     5761 cggatttggt gagaataacg ggagtaagtc gtaattctat taataagttg tggcacaatg
     5821 agaatgtgtc ttctttaaaa ttagatacat tgataacgat ctgtgagaag ttggatgttg
     5881 agttattgga tttaattgaa tatattcgag atgatagtga gacaaaataa tttaggggct
     5941 attttagctt tagatagctt gtgtatgatg aaagcatatt tttgctaatc gacaatgagt
     6001 tatattcaca tatgtttttt ttgtagggct atatgtgtat ttaaagtttt gatttctatt
     6061 tgttattgta tgaatatggt ttgaatgtgg taaatgaaat tatagtcttg ctaggtgcgt
     6121 caacaccttg gaagatgagc ggtactggca tacttcgtga tttcattgat agtagtgcag
     6181 taaagtgatg gaattgatga gtattgaaaa agagttcctg tttgggggct ctttttttat
     6241 ttgttactaa gtatttttaa atagtgaagg tgctacacta tttaaaaata cagaatatta
     6301 aaaactttat atatcaactg ttgaaaagtt agtcttatac aatagcatga taacatgata
     6361 gaaaataaaa tatagataat tttggaaaag gagaagattc tatgaatgtt caactaggaa
     6421 acgaacaaat tacaaaatta ttaaatgatt ggtatcaaga aattcgagcg caacacatta
     6481 ttaaagcaaa acaattaaaa caaaagattg aaaaagaaat taacaacata gaagaagacc
     6541 aaaatttact tttatattat tcacttattg atttgagata caagattctt acaaacgact
     6601 atgcagatag agaacaatct ttagaaaaaa tcgaacaatt aaaagaacac acaaataatt
     6661 ttttagatta ctattatcat ttctttaaag gcatgcatgc aatgaaaaca ggaaattata
     6721 gtgaaactca aaaatattat gatatagcag aaaaattact agaagaaatt cctgatgaag
     6781 tagaaaaagc tgaatttaat tatgctgttg caactttcta ttatcatact catcaagcct
     6841 tattagcaac tcaatatgct aacaaagcca aaagtttttt tagtgggaaa ctaggatacg
     6901 agattaaaac cggagcatgt gaaaacacac ttggtatggc ttgtattaca ttaagggaat
     6961 tttctgttgc agaagaatac ttgctttccg caattaataa atttgaaaaa cacagtgaaa
     7021 acaaattagc tttaatcgta cgttataact taggtctttt atatgcagat caaaatcttt
     7081 ctgaacttgc aatccgttat cttcttgaat cctttgaaaa tcaagaagac gattacaaaa
     7141 caatgttttt attagcacaa gaatattaca agttaaaaca aacaaataca gtaaacacat
     7201 atattgaaaa agggctaaat gtttgtaacc aagaatataa aattcatttc ttatttttac
     7261 aagcattaaa caataaatta tctactgaac aattagaaaa agtcatgtta gacgcgattc
     7321 catattttga aaaacaaaat ctatggaaat acatacaaga ttatactgaa gaattggcta
     7381 taagatttta tgaagaaaag aataaggata aatctaatga atatttttat aaaagttaca
     7441 aagctaaaag aaacttatta gaaagggagt cattgaaatg atgaaaaaat ttagtctgat
     7501 actaataggg gtagcatgta caacaggtat tttcttttcc caattcaata attccattca
     7561 aacacatgat gctaaagaaa aaaatgatat tattcaacaa tatgcacatg gaaaagatat
     7621 ttaaaagcaa attccaacaa ttagcaatcg acctaaatat taggattatt aacaaaatat
     7681 tgaattatat gtaaattaag tttattatct attttgaata ataaacttaa ggggatgaaa
     7741 tttatgaaaa aaattagtat tgagagaagt aattggagaa aaacatataa aaaaatgatt
     7801 ccagtattgt tagtcggagg aatattggca ggaaatgtag catttgtgac aaataatgtt
     7861 tttgcggcaa ctacaaataa caacaatata atacaaacga aacagtctct cgtttctggt
     7921 caagatgaaa agagcattaa tgaattagca gaagcgtttc aatttatgca tgaagaagcc
     7981 gctatatata ataatcaagg tgaaattgtt gattttgatt ttaataaaat agaagcgaaa
     8041 tatgggaatg atgttggttt agatgcactt aaacaacaaa ttaaaaaaga taaaagtgat
     8101 attgctggtt tatcttttac aagttgcatg aaagatgctt tatatgactt ttttggagta
     8161 aatgctgtac aagccgcatt taatggtgga ttggcatatt atcttgagaa aaaattatgg
     8221 aaagaagcgg ctaaaatagg cgttaaattc tttataggaa gtagcgtagc aggtttagcg
     8281 gctactttag cttattatgg aggtaagtgt gcaatttatg gataaaagtt caattttaaa
     8341 acaaatgtat tttattttat ttccggtaac actttttcta tctctaaata tttatcaatg
     8401 gattgttaaa agtgattact catttacagg atatataatc caaaatttaa ttattgtagc
     8461 attagcgtta gtagcagcta tcatagctta tttaattact aaaaaaaata ttt
//
