LOCUS       NZ_CP083100             5535 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain B401 plasmid pP05_2-o, complete
            sequence.
ACCESSION   NZ_CP083100
VERSION     NZ_CP083100.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214334
            Assembly: GCF_020809125.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x, 60x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809125.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,136
            CDSs (total)                      :: 6,981
            Genes (coding)                    :: 6,638
            CDSs (with protein)               :: 6,638
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 343
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 343
            Pseudo Genes (frameshifted)       :: 120 of 343
            Pseudo Genes (incomplete)         :: 233 of 343
            Pseudo Genes (internal stop)      :: 92 of 343
            Pseudo Genes (multiple problems)  :: 78 of 343
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083100.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="B401"
                     /isolation_source="Biopesticide B401/B402 (Certan),
                     Vita-Swarm"
                     /db_xref="taxon:1428"
                     /plasmid="pP05_2-o"
     gene            complement(2..400)
                     /locus_tag="K8Z39_RS35445"
                     /old_locus_tag="K8Z39_35305"
     CDS             complement(2..400)
                     /locus_tag="K8Z39_RS35445"
                     /old_locus_tag="K8Z39_35305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1878 family protein"
                     /protein_id="WP_021728521.1"
                     /translation="MNIEQEISKLKYHKELMLTMLLHENPDKFSFYHQIINHDLEKKDE
                     IAILNIISLFNKRLSNENTFDFEKEFFDSISLQVIYDCNVKPTIEEYEGYLKAINIPIN
                     PKYLLMAINKQKESDNVCEFLLEKYKEK"
                     /name="DUF1878 family protein"
     gene            complement(424..888)
                     /locus_tag="K8Z39_RS35450"
                     /old_locus_tag="K8Z39_35310"
     CDS             complement(424..888)
                     /locus_tag="K8Z39_RS35450"
                     /old_locus_tag="K8Z39_35310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011839882.1"
                     /translation="MLKIKSKFKKIIPATMAFAALLTVAPLSSFAAETETPVKNNVVNF
                     PQTNYKVENVNQTVSLDEDGDVSAQGWKKSAVVWGLKNGGDALQTLTKWLGAGTKEAKY
                     LNKHAKSIANALDRFESHIEQNLIDFMIFECGIPGGSARVIASAITGLVL"
                     /name="hypothetical protein"
     gene            complement(1480..1932)
                     /locus_tag="K8Z39_RS35455"
                     /old_locus_tag="K8Z39_35315"
     CDS             complement(1480..1932)
                     /locus_tag="K8Z39_RS35455"
                     /old_locus_tag="K8Z39_35315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021728522.1"
                     /translation="MKKKEILEVTEMLSTFIDAGAGAQELMKAITERNLHKNSNFIFVF
                     HTLKAWIESDNPEVKLSKTEKAVLFTLLPYVGWNNQIELLQPTMQKLCGYKDRSYFSKV
                     LNSLVRKNVLEKQSVGKQNYWFFNSLLVKKGNSKERKPYIKFELKD"
                     /name="hypothetical protein"
     gene            complement(2180..3505)
                     /gene="mobV"
                     /locus_tag="K8Z39_RS35460"
                     /old_locus_tag="K8Z39_35320"
     CDS             complement(2180..3505)
                     /gene="mobV"
                     /locus_tag="K8Z39_RS35460"
                     /old_locus_tag="K8Z39_35320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_021728523.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELISLQQDF
                     PLEMREKGFDVDRGEGSEKKHLSPQAFKEKQDLEVEVEQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLSLDKKIKEKKQEFETFRNQIPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMKKKVDAAVIIKSDYERLQKTDLVKENKELHS
                     AVDEICDSLKESQKRNVGLQKENKQLSTEISSLKAHIRDLQTNIKVLYQQTKQVFKEQF
                     KTFRGLVKNELVGREVENHFEREHERENKKKISRHRGYDMER"
                     /name="mobV"
     gene            complement(3911..4207)
                     /locus_tag="K8Z39_RS35465"
                     /old_locus_tag="K8Z39_35325"
     CDS             complement(3911..4207)
                     /locus_tag="K8Z39_RS35465"
                     /old_locus_tag="K8Z39_35325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021728519.1"
                     /translation="MAKEQIKLYVSEEEKERLELLAKMNSTTVSNYVRMTALGVRVRPP
                     KEIPVVVEKERLEDEDKLVLQELLERTQGKKDFITLDREFNTRLIALAGRLLE"
                     /name="hypothetical protein"
     gene            complement(4369..5490)
                     /locus_tag="K8Z39_RS35470"
                     /old_locus_tag="K8Z39_35330"
     CDS             complement(4369..5490)
                     /locus_tag="K8Z39_RS35470"
                     /old_locus_tag="K8Z39_35330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="WP_021728520.1"
                     /translation="MKKFIMIAVLLFGLCSGSVTSFASELSKKDDYLIMFRDKIDENLI
                     EKQGGEVKETYSNLPIVKAEFPYDPTKTLKQYFEVLEVEQDGNVKANGTKQSLHNGVSS
                     VLSTKGKGVSPLTGKGIAVAVLDTGIDREHPDVKIKDGVSFVENVLDFDDDNGHGTHLA
                     GIIGAQKNNSGMTGIAPDVDLYAVKVLDKYTNGKYSTVVKGIDWAIEHKANIILMSLGG
                     KKESVFFEEAINKAYEKGILLVSSAGNEGYKEGDSITYPAKFNSVIAVGALDAKDQRGF
                     LSSKGEQLELMAPGVDIWSTWNDGSYRFDSGTSMAAAHVAGVASLLLENDRMLSNQQVR
                     ELLKKSATPLGSPFEYGNGKINLSHALQMMNQK"
                     /name="S8 family peptidase"
     Typing          complement(2184..3505)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.546"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttatttttc tttatatttt tctaataaaa attcacaaac attatctgat tctttttgct
       61 tattaattgc cattaataaa tatttagggt ttatcggaat atttattgcc tttaaataac
      121 cttcatattc ctcaatagtt ggttttacat tacaatcata aattacttgt aaactaatag
      181 aatcaaaaaa ttctttttca aaatcaaatg tattttcatt agataacctt ttgttaaata
      241 aagaaataat atttaaaatt gctatctcat cttttttttc taaatcatgg ttaataattt
      301 gatgataaaa agaaaattta tcagggtttt catgtaaaag catagttaac attaattctt
      361 tatgatattt tagcttagaa atttcttgtt caatattcat attaaacacc tacaattcaa
      421 taattataaa acaagaccag taattgcact tgcaataact cgagctgatc caccaggaat
      481 tccgcattca aaaatcataa aatcaattaa attttgttca atatgacttt caaaacgatc
      541 taatgcatta gcaatacttt tcgcatgttt attcaaatat tttgcttctt tagttccagc
      601 acccaaccat tttgtaagtg tctgtaacgc atctccacca ttttttaagc cccaaacaac
      661 agctgatttt ttccaccctt gagctgatac atcaccatct tcatctaaag aaacagtttg
      721 atttacattt tctactttat aatttgtttg tgggaaattc actacattat tttttacagg
      781 cgtttctgtt tcagctgcaa aagaagataa aggtgcaact gttaaaagag ccgcaaatgc
      841 cattgttgct ggaatgattt ttttgaattt agatttaatt tttaacatgt ataattcctc
      901 cagaaataaa tttttttaac aaagataagt atgtcatatt tcaaaactct tttttctgta
      961 tatgtaaaat tgcatatact tttggaaaac aaaattagta atccaatgtt gagtttccct
     1021 gtttttgggt gtgagtgctg gctaggggtt tgggggctag cccccaagaa cgtaatttat
     1081 tatcggataa gtttatcact tggaacgata gaacttggtg gttcggctgg tataattacg
     1141 agtccaagtc gagcaattta atcccgtttt tggtctgttc ggcttggcga acgctaaaaa
     1201 ccagccgaat tttggaatgc aatggaagaa aggtggagat tttttgagag tttttttcaa
     1261 aaaatactac ctgaccattg tatggagaaa aaccaacccc ttatatagat atagaaagaa
     1321 caacatggaa tttttaataa aaaagtgaat aaacatgatt atatcagggg ttctagcgat
     1381 tgtaattaat tcccaatatg tggggtaaaa accacatata tgtggggtaa aaaccacata
     1441 ttgggaaaat atgtaaataa aaaagactag taatttttac tagtctttta gttcaaattt
     1501 tatatatggt tttcgttctt tagaattacc ttttttaaca agtaaagaat taaaaaacca
     1561 ataattttgt tttcctacac tttgtttttc taaaacattt ttccttacta atgaatttaa
     1621 aaccttacta aaatatgacc tatctttata accacatagt ttttgcatag ttggttgtaa
     1681 aagttcaatt tgattattcc aaccgacata aggtagcaaa gtaaataaaa ctgctttctc
     1741 tgttttacta agcttcactt ctggattatc actttcaatc caagctttta acgtatgaaa
     1801 aacaaatata aaatttgaat tcttatgtag atttctttca gtgatcgcct tcataagttc
     1861 ttgagcacct gcaccagcgt caataaaagt actcaacatc tctgtaactt ctaaaatctc
     1921 tttctttttc atttgttgtt gctcctctca atattccgag caccaacaaa aataccaaaa
     1981 atcataattc aggtattacc ctaataataa aattatgata atctatatac gacaacaagt
     2041 tattggtatt ttcgctgaaa taccctttga aacgagccat gttcgagcat ggctcgtttt
     2101 ttaattttat cacacatatt ctctcaaaag attgtcaaat aaagtttccc aaaagtttta
     2161 tttacttttt gaatgtcttc tatcgctcca tgtcataccc tcgatgtcta cttattttct
     2221 tcttattttc acgttcatgc tcccgttcaa aatgattttc tacctctctt ccaaccaatt
     2281 cattttttac aagccctcta aacgtcttaa attgctcttt aaagacttgt ttcgtttgtt
     2341 gatataaaac ctttatattt gtctgtaaat ctcttatatg agcttttaat gagcttattt
     2401 ctgtacttaa ttgtttgttt tccttctgta accctacatt tctcttttga gattctttaa
     2461 gtgagtcaca tatctcatcg actgcactat gtaattcttt gttttctttt acaagatccg
     2521 ttttttgcaa acgttcataa tcgctcttta taataaccgc tgcatcaacc ttttttttca
     2581 tatctctcca ttgctcacgt gtcaccacga tgtttcctgt tttcttctca gtaatttctg
     2641 gctttccaaa cagtttcgtt gttacttctg tttttgtttc ttctcgcaaa taagacattg
     2701 acactggctt atccggaatt tgatttcgaa acgtttcaaa ttcctgtttc ttttctttta
     2761 tttttttatc taaactcaaa aattgttgct gaattttttg taacgtttca ttctgttttt
     2821 cgatgtagtt tgtggtttgt tgtaattgat tatgcgtttc cactactttt gtctttaaat
     2881 gcgtttttac attggataac tgttccactt ctacttctaa atcttgtttt tccttaaagg
     2941 cttgaggcga taaatgtttt ttctcagaac cttctccccg atctacatca aaaccttttt
     3001 cccgcatttc caacgggaag tcctgttgca aagatatcaa ttcttttctc gtaaacatat
     3061 cttttgcgga caaacgattg tcttctgtaa ttggcatcac accaatatgc atatgtggtg
     3121 tcgcttcatc catatgaacc atcgcataca acgtattttg tttgccataa cgcttttgaa
     3181 taaaatctag tgaactctca aaataacgtt tctgttcttc taaacttaat gaatgcatgt
     3241 aatcaggcga agctgttact acaaattctg aaagcacaac agaatttgcc cgtggctttc
     3301 gttttactcg ctcactgatc tcttgtttaa ttgtgctttc atatctaatt ttttgttgat
     3361 tcaacaaatc ataattctgt tcacttttcg aataatcgat gtctaaattt gatttacttt
     3421 ttccttgtct ttgattatgt ttttgaatcc cttgcacatc agaaatttga aatttctgca
     3481 tgtgaatgat tgcgaatttg ttcatgtttt tccctcacaa ttgtcttccg taagaggact
     3541 acttttaagg ttatcataac atatcgagag agcgaatcca aagttttggt atagcccact
     3601 ataccaaatt acatttggtg tgggctctgc gagaccttgt tggcttcgcc aaatcacgcc
     3661 atagcgtgac aaatgctagg ggctttgccc cacaccccac aaggggaact tttccccttg
     3721 accccatcca tcgaactccc caacccctca atccttgagg ggctccctcg ccggttggcg
     3781 tccccaaaac aatatgtttt gaggaactgc caagagggtg atacatctga cgtacgtgtc
     3841 cactccttat cctcccgatc cctttcgggc tcggtccggt accgttgaca cttacctcaa
     3901 aatttcctct ttattcgagt aatcgtccag ctagtgcaat taaacgtgta ttaaattctc
     3961 tatctagtgt aataaaatct ttttttcctt gtgttcgttc aagcaattcc tgtaacacca
     4021 atttgtcctc atcttctaat ctttcttttt caacaactac aggaatttct ttaggtggtc
     4081 taacacgaac acctaaagct gtcattctta catagttcga aactgttgtg ctattcattt
     4141 tagctaataa ctctaatcgt tctttttctt cttcagaaac atataatttg atttgttctt
     4201 tcgccatttt taacatcctc ttagggtata ttttgttcct ttcatcatat ccctttatat
     4261 accctttttc aatatataat ttatagtcct ttttataccc tttaatactt atcccttcta
     4321 taccccttta aaaaataaaa gagagccaac tataaagttg gttctctttt atttttgatt
     4381 catcatttgc aaagcatgac ttaaatttat tttcccattt ccatattcaa atggacttcc
     4441 taaaggagtt gctgattttt ttaataactc tcttacttgt tgattagaca acattctatc
     4501 attctctaat aacaaagatg ccacaccagc tacatgagcg gctgccattg aagtaccact
     4561 atcaaatcta taagaaccat cattccaagt actccaaata tctacacctg gtgccattaa
     4621 ttctaactgt tctcctttac tagataagaa ccctcgttga tctttagcat ctaacgctcc
     4681 aactgcaatt acagaattaa atttagctgg ataagtaatt gaatctccct ctttatatcc
     4741 ttcattccct gctgatgaaa ctaacaaaat tcctttttca tatgctttat taatggcttc
     4801 ttcaaaaaat acagattctt tttttccacc tagactcatt agaataatat tcgccttatg
     4861 ttcaattgcc caatcgattc cttttacaac tgtactatat tttccattcg tatacttatc
     4921 taatactttc actgcatata aatccacatc aggagcaatt cctgtcatac cgctattatt
     4981 tttttgcgct ccaataatac ccgctaaatg tgttccatga ccattatcat catcaaaatc
     5041 taaaacattt tcaacaaatg aaacgccatc ttttatcttt acatctgggt gctctctatc
     5101 aataccagta tctaaaaccg caacagcaat tcctttccca gtaagagggg aaactccttt
     5161 tcctttcgta gataaaacac tggatacccc attatgtaaa gactgtttcg tcccatttgc
     5221 ttttacattt ccatcttgtt caacttccaa gacttcaaaa tactgtttca aagtctttgt
     5281 tggatcataa ggaaattcag cttttacaat gggtaaatta gaatacgttt cttttacttc
     5341 cccaccttgt ttttcaatca aattttcatc aattttatct cgaaacataa ttaaataatc
     5401 atctttcttt gataactcac tcgcaaaaga agttacactt ccactgcata gtccaaataa
     5461 caatacagct atcataataa attttttcat atttttgatc ctccattaca cataaaaaat
     5521 aaggtgcaaa agcac
//
