LOCUS       NZ_CP083099             9174 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain B401 plasmid pP05_2-n, complete
            sequence.
ACCESSION   NZ_CP083099
VERSION     NZ_CP083099.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214334
            Assembly: GCF_020809125.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x, 60x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809125.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,136
            CDSs (total)                      :: 6,981
            Genes (coding)                    :: 6,638
            CDSs (with protein)               :: 6,638
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 343
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 343
            Pseudo Genes (frameshifted)       :: 120 of 343
            Pseudo Genes (incomplete)         :: 233 of 343
            Pseudo Genes (internal stop)      :: 92 of 343
            Pseudo Genes (multiple problems)  :: 78 of 343
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083099.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="B401"
                     /isolation_source="Biopesticide B401/B402 (Certan),
                     Vita-Swarm"
                     /db_xref="taxon:1428"
                     /plasmid="pP05_2-n"
     gene            complement(join(9151..9174,1..240))
                     /locus_tag="K8Z39_RS35385"
                     /old_locus_tag="K8Z39_35250"
     CDS             complement(join(9151..9174,1..240))
                     /locus_tag="K8Z39_RS35385"
                     /old_locus_tag="K8Z39_35250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228225090.1"
                     /translation="MSRERVKIKFKSEDIKAEIEGYSDMSYWNHDLKLEFILRLLNTVD
                     EKQFLESQNHDQLDYYTKEMIKMIEASLGFDGLYRLVYGDEL"
                     /name="hypothetical protein"
     gene            complement(472..909)
                     /locus_tag="K8Z39_RS35390"
     CDS             complement(472..909)
                     /locus_tag="K8Z39_RS35390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228225091.1"
                     /translation="MKNQSQVEELIDKVDSYFVENGLDSNDKFSHPEIEEMESKLEDFK
                     ALIEERLAEQITEKDFLKQEVSKLFKEIDFLKKQVQILTKKNWFKAFCSKTGKFIRNNP
                     ETTKAIAHTAKECLPESIKMSIPEGATEVMNTLLEEQKVEN"
                     /name="hypothetical protein"
     gene            complement(872..1234)
                     /locus_tag="K8Z39_RS35395"
     CDS             complement(872..1234)
                     /locus_tag="K8Z39_RS35395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228225092.1"
                     /translation="MLTNTFLSKVYFALDYSDFFEIDDFSVTLPEEKSYSQELIIKYLF
                     DETCYFHTYINGSSEFRYECSPGKVMKIQKGNVSGVDIYTTEIRDWVKRMEDEFKTTPF
                     SKESNEESISSRRIDR"
                     /name="hypothetical protein"
     gene            complement(1568..1849)
                     /locus_tag="K8Z39_RS35400"
                     /old_locus_tag="K8Z39_35260"
     CDS             complement(1568..1849)
                     /locus_tag="K8Z39_RS35400"
                     /old_locus_tag="K8Z39_35260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228225093.1"
                     /translation="MEYFHSFGVNFINTVKDLAEKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNEQIMGLGEGLSDISKSLSDMSESIALVSLDDKE"
                     /name="hypothetical protein"
     gene            complement(1881..3086)
                     /gene="mobV"
                     /locus_tag="K8Z39_RS35405"
                     /old_locus_tag="K8Z39_35265"
     CDS             complement(1881..3086)
                     /gene="mobV"
                     /locus_tag="K8Z39_RS35405"
                     /old_locus_tag="K8Z39_35265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_228410769.1"
                     /translation="MSRFMVCHMQKFKMTDVKGLQIHNQREKESHSNPDIIQERTEQNY
                     DLIHGKENVDYKKLIQDRIQDGVVSDRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSAQRFKAVTLQQEIEKLEQEKNEIDSRLHDLKL
                     SLDKAKSVDEIPVKEKGGFIRSKTVEIALEDFESIKVLAKSSETLREENKRLKNEKVKS
                     EYEKDNLYKEQRFLERKVTDLKRENEGLKGENDFLKKTLDRVKDLYKEKFPEFAGMIGY
                     VKASILDKMNRKFLKRHFAGDDEVSGAQKFFNHKQEHEEQQKRLKQVRRSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            complement(3716..4384)
                     /locus_tag="K8Z39_RS35410"
                     /old_locus_tag="K8Z39_35270"
     CDS             complement(3716..4384)
                     /locus_tag="K8Z39_RS35410"
                     /old_locus_tag="K8Z39_35270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002081870.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_001270695.1"
                     /translation="MRYSDIKVGNIYYADLNPIRKYEFGDNHLSIILSKGKDKRTVTIV
                     SLTSKSSGLGQNKMNLGIVSGLPKRLVEDRSGNPINTYVVLDQVRTVSANRIQYIKDGK
                     KTDGTDNYIECPVDAFSFSKIVCELADLRIADLNDEDAIGEYHKETFFNYCVKKMIDLT
                     YDIIKGRGIVADKKEEVIYFYNNALAMEKGFLIDNYLKPHDIKNKVLEKFNEIVLMSVK
                     "
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(4959..5186)
                     /locus_tag="K8Z39_RS35415"
                     /old_locus_tag="K8Z39_35275"
     CDS             complement(4959..5186)
                     /locus_tag="K8Z39_RS35415"
                     /old_locus_tag="K8Z39_35275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000780193.1"
                     /translation="MKLVNNIRMIMAQKNIDNIAELIRITGVSRNSINKLWHNESVSSL
                     RLDTLIAICEKLDVKLSDLIEYIPGDIESK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5374..5661
                     /locus_tag="K8Z39_RS35420"
                     /old_locus_tag="K8Z39_35280"
     CDS             5374..5661
                     /locus_tag="K8Z39_RS35420"
                     /old_locus_tag="K8Z39_35280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228225087.1"
                     /translation="MAYLTAKKVKGNIYFYVSQYVGTQPYYSKHYKYINIYSIGNQKIA
                     LERIAMWLLDNNRIPKELIEIGVSINDVKYWYEKVEKTLQNYSLTNHKNI"
                     /name="hypothetical protein"
     gene            5675..5938
                     /locus_tag="K8Z39_RS35425"
                     /old_locus_tag="K8Z39_35285"
     CDS             5675..5938
                     /locus_tag="K8Z39_RS35425"
                     /old_locus_tag="K8Z39_35285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874966.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(6683..6844)
                     /locus_tag="K8Z39_RS35430"
                     /old_locus_tag="K8Z39_35290"
     CDS             complement(6683..6844)
                     /locus_tag="K8Z39_RS35430"
                     /old_locus_tag="K8Z39_35290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228225088.1"
                     /translation="MLSEQVIYYWNDELQYYYLAEKKNGSYKFVQPLKKLELDDISDPL
                     EREQMKKC"
                     /name="hypothetical protein"
     gene            complement(7275..8657)
                     /locus_tag="K8Z39_RS35435"
                     /old_locus_tag="K8Z39_35295"
     CDS             complement(7275..8657)
                     /locus_tag="K8Z39_RS35435"
                     /old_locus_tag="K8Z39_35295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000648.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_228225089.1"
                     /translation="MNDVLESSLLDATIDKLSIVADFKEGIKKEEFIRMVASSNMPYSL
                     QANRSKNFGYEEVLKCDSVDCGYIELSGELKKASVDLPKLISHRIRLEIQIEQEKKARE
                     VGESLLSNAEYQGLFESLADVKELIAETDEDGRLTDERKLKRHERQIELTIEKLKGEII
                     ALKSEIDEQELATGFKNEAMEKIHEEHVQILLNTLENKEKLIEVKKDELLRNAEMFLQL
                     DANGFLPNNGKKKNDRLLKDVRFEFNPKHMAYNRVVDESIRLVLGLLENVEVTGIHIAC
                     DYPIKIAELKIKDLSSKSECIFLDRDKSLETMYVGKRASDNHLCIYDKKRENEENDTLD
                     QYPDVEHVTRFEARLRKKKASEWMESTYNPFDSIIVGDLVSLDGSEMKLGDRIVLEAIM
                     NDYHGRYFGMMDKFQRSTWRKKLKQYVPKLFDVAGDYEKKKVLLTGQLAELLENGKNKV
                     GK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(8821..9027)
                     /locus_tag="K8Z39_RS35440"
                     /old_locus_tag="K8Z39_35300"
     CDS             complement(8821..9027)
                     /locus_tag="K8Z39_RS35440"
                     /old_locus_tag="K8Z39_35300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000030440.1"
                     /translation="MSKANYVTVGRKPKDGKNPKIPETFRLPKETSERIKGLVSSGRFS
                     TKAEIAEKAFEFYFSYLEKENGK"
                     /name="hypothetical protein"
     Typing          complement(7276..8657)
                     /name="rep_cluster_268"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.085"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1885..3086)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.277"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgatataat ccatcaaacc ctaaactagc ttcaatcatt ttaatcattt ctttagtgta
       61 ataatccaac tgatcgtgat tctgactttc aagaaattgt ttttcatcaa ctgtatttaa
      121 aagtctcaaa ataaattcca gtttcaaatc atgattccaa taagacatat cagagtaacc
      181 ttcaatctcc gcttttatat cttcagactt aaattttatt ttcacccgtt ctctagacat
      241 acttatccct cttttcccta gttataaata ttagaaacat caataaaaaa acatcacaga
      301 ccattgatac caaagtattt tctaataaca tcacccactt cgcgccccga gatgtttcta
      361 atatatcaag taatataatt aatccttttc tttaacaata aaaaaataga actcttgcat
      421 aataaatata aacaagagtc ctattaccat taaatatttc taattcaaat tctaattttc
      481 aaccttttgt tcttccagca aagtgttcat tacttcagta gccccttctg gaatactcat
      541 tttaatagat tccggaagac attctttagc tgtatgggca atcgcttttg tagtctcagg
      601 attatttctt ataaacttac cagttttact acaaaatgct ttaaaccaat tctttttagt
      661 taatatctgt acttgctttt taaggaaatc tatttcctta aaaagtttcg aaacttcttg
      721 ttttaaaaaa tctttttctg taatttgttc agccaatctc tcttcaatta aagctttaaa
      781 atcttctaat ttgctttcca tttcctcgat ttccggatga gaaaacttgt cattagaatc
      841 caaaccattc tctacaaaat aagaatccac tttatctatc aattcttcta cttgagattg
      901 attcttcatt actctccttg ctaaagggtg tagttttaaa ctcatcttcc attcgcttta
      961 cccaatcccg aatctcagta gtataaatat ctaccccaga aacattcccc ttctgaatct
     1021 tcataacctt tccaggactg cactcataac gaaattccga tgatccatta atataagtgt
     1081 gaaaataaca agtttcatca aacaaatact ttatgattaa ttcttgagaa taactttttt
     1141 cttctggaag tgtcactgaa aaatcatcaa tttcaaagaa atcactatag tccaacgcaa
     1201 aataaacttt acttagaaac gtattagtta acacaaaaaa tcctcccaac ttgtatatac
     1261 ccaacaataa aaatacacaa aataaaaaca ataaagtcac acaagacttt actatcctca
     1321 ttaaaattaa atataccaat atctaacaac ataatacaca tctaaaaaca cttatttcaa
     1381 tttccttttt tttaaaacaa gagccatccc caatcccctt gtataatatc cccatatatt
     1441 cacatcaaca atttacgagg acgttataca aggctctaca atcctcgcta tatttcacaa
     1501 aaataagttt cactaatgct aacttctaaa gaaaaaaggg aactcccata gagtccccaa
     1561 cacactatta ctctttatca tccaaagata ctaacgcaat actttcactc atatcactca
     1621 aacttttact tatatcactc aagccttcac caagtcccat aatttgttca ttcatctctt
     1681 tgataagttc tccaatacca gctaattgtt catccttacg atcatagtca tcaattttca
     1741 tagtcaattc caaaatcctg caagtctctt caatcgttaa attatttttc tcagccaaat
     1801 ccttcacagt gtttataaag ttcactccaa agctatgaaa atactccatc cttggttgtt
     1861 gattaaataa tgtccccaca ctatctctcc aatccctggt cccgattttt ctgttggctt
     1921 cttcgcactt gttttaatct cttttgttgt tcttcatgct cttgtttgtg attaaagaac
     1981 ttttgagcac cacttacttc gtcatcacca gcaaaatgtc gttttaaaaa cttgcgattc
     2041 atcttatcca gtatactagc tttaacataa ccaatcattc ctgcaaattc agggaacttc
     2101 tccttataca aatctttcac tcgatccaac gttttcttaa gaaaatcatt ttcgcccttt
     2161 aacccttcat tctctctttt taaatcagtt actttcctct ctaaaaaacg ttgttcttta
     2221 tataaattat ctttttcata ttcactctta accttttcgt ttttaaggcg cttattttcc
     2281 tctctaagcg tttcagaaga cttagccaat acttttatac tttcaaagtc ttctaatgca
     2341 atctcgaccg ttttagagcg tataaaccca cctttctctt taacagggat ctcatctaca
     2401 cttttagctt tatctaaaga taatttcaaa tcatgaagac gactatctat ctcgttcttt
     2461 tcctgttcta acttctctat ttcttgttgc aaagtcaccg ctttaaagcg ctgcgccgat
     2521 aaatgtttcc tgtcactaga aacaccacgc tctatatcaa atcccttttc tttcacatgt
     2581 tcatagattt tatcttgtag atcatgaagc tctgatttgt ttttaaaaaa tgaatacgct
     2641 gatagttttt gtttttcatt aaccggaacc attcctacat gcatatgagg tgttttttca
     2701 tcaacatgca ctgaagcata tacaaagttc tcttctccat accttgcctt taaaaaacaa
     2761 acagactctt caaagaatcg tctctgttcc gcttgagata aagaattcat ataatcagga
     2821 ctagccgaaa tcataaacga acaacaaaca acagcatctt tcctaatggc tctatcagat
     2881 acaaccccgt cttgaattct atcttgaatt agttttttat aatccacgtt ctctttacca
     2941 tgaatcaaat cataattctg ttccgttcgt tcttgaataa tatctggatt tgaatgactt
     3001 tctttttcac gttgattatg tatttgtaat cctttcacat ctgtcatctt gaatttttgc
     3061 atatgacaaa ccatgaatct tgacatgggc ttcgctcctt tttcggtggt agttccatgt
     3121 aaactgtagc acactacagt ttactgcgta aacacgtgtg ccctacgggg catttcggct
     3181 accaccgagc catgacaatc atggcaaaaa accatgggct ttgcccaaac ccacaaggga
     3241 aaaacttccc cttgacccct tacaatcgaa ctccccaacc cctcaatcct tgaggggctc
     3301 cctcgccggt aggcatcccc aaacgttcgg tttgggaaaa ccaagagggt tttgttgtag
     3361 taaagatgtc aactccttat cctcccttcg gtccggtacc gttgacatcc cccacacaaa
     3421 gttcatatct ctttaaaagt aatccccaat aaaaaaagcc ccctttattt tagagttaaa
     3481 accctcaaaa caaaggaagc catcattttc ataaataaca atcaaaaaga cctttctttt
     3541 tacagaaagg tctctctatg gttgagccat cacagctcgc caactattgt tcgggtcatc
     3601 acaaccctaa attctacggt tgagccatca cagctctttc gcaattagta ttaactaacg
     3661 ctacttcttc taaccacact gtatcaaaaa taataataaa aatcaacact tataattatt
     3721 ttactgacat taaaacaatc tcattgaatt tttccaacac cttatttttt atatcatgtg
     3781 gttttaaata attatcaatt aaaaaacctt tttccatcgc taacgcattg ttataaaagt
     3841 atattacttc ttctttttta tccgcgacga tccctcgtcc cttaataata tcgtatgtta
     3901 aatcaatcat tttctttaca caataattaa aaaaagtttc tttatgatat tcaccaattg
     3961 cgtcttcgtc attcaaatca gcaatcctta gatctgccaa ttcacataca attttagaaa
     4021 aagaaaacgc atcaactgga cattcaatat aattatcagt accatcagtt tttttgccat
     4081 cttttatata ttgaatacga ttagcggaca ctgtacgaac ttgatccaaa acaacatatg
     4141 tattgatcgg attcccactt cgatcttcaa ctaaacgttt aggtaatccc gatactatac
     4201 ctaaattcat tttattttgt cccaaaccag aacttttcga agtaagagaa acaatagtca
     4261 ctgtcctttt atctttacct ttacttaaaa ttatacttaa atgattatct ccaaactcat
     4321 atttccttat tggatttaaa tccgcataat atatattccc cacttttata tctgaatacc
     4381 tcaacaatag ttccccctat aattaattac ttattcacaa gtttttacct ttaaaacata
     4441 aacaattgga ctatattcag ctttcaatcc tcatcgctac acacttgtcc attacccttg
     4501 tacaatatag taacttctct ttttttttgg taaatttgat cacctacaaa tagcttttaa
     4561 ccattttttt aaatctcaca taccaagtta aatttagctt aacataacaa taaacaattc
     4621 gcttttcaaa aaatgctcta tcttgttcaa taaacaaaaa acataaaaaa gagccctgaa
     4681 aataggactc tttttaataa tcatcattac caccatttca tttaacagca ctactatgat
     4741 tataaatata gttcaacaaa atcacgaagt atgccagtac ctctcatctt ccaaggtgtt
     4801 gacgcaccta gtaagtctac aattccatat ccctaattca aaacatactc atgcgttaac
     4861 aacagaagtt gaagtgcgcc atatagcacc accaaatacc attagccaac tataccttta
     4921 gcatacaaaa actatccaag cacataaaat cccctaaatt attttgactc aatgtcacct
     4981 ggaatatatt caattaagtc cgataattta acatctaact tctcacagat cgctattaaa
     5041 gtatccaatc ttaaagacga cacactttca ttatgccaaa gcttattaat agaattacga
     5101 ctcacaccag ttatccttat taactccgca atattatcta tatttttttg ggccataatc
     5161 atacgaatgt tatttactaa cttcattcct cttactcctt atattcaata tacactccat
     5221 tttacttata aagataattt tatgcaattc ataaataaaa ttaaccctca agaaaattat
     5281 ttttatttat atgtattgat attttaactt tataaggtta aaatataacc acagacaaaa
     5341 acaaaaaaca acaaacatga aaggaagaac gatttggcat atctaacagc aaaaaaagta
     5401 aaaggaaaca tttactttta tgtatctcaa tacgttggaa cacaaccata ttactcaaaa
     5461 cactataaat atataaatat ttactcaatc ggcaatcaaa aaatagcttt agagagaatc
     5521 gctatgtggt tattagataa caaccgcata ccaaaagaat taatagaaat cggagtatct
     5581 ataaacgatg tgaaatattg gtatgaaaaa gttgaaaaaa cattacagaa ctactcttta
     5641 actaaccata aaaatatcta aaaggagcat tactatggca tttctaacaa attacaaagc
     5701 aaacggaaaa agatactttt atgtagaaaa atatgttggc aaaaaaccat atacctgcaa
     5761 acaaagtgaa cgtatttatt cgattggaaa cgaaagaatc acattagaaa ggctcacttt
     5821 atggatttta gataacagtt tcatcccaaa cgaattaata aaaatcggaa tatcaattga
     5881 cgatatagag aactggagag aaaaagtaga aaataccata aaaagatact ctctataaca
     5941 aaaaagactg atatatgtat cagtcttttt ttctttaatt gtttatattt ttttcatcac
     6001 aaaagggtta ggtattttaa taatatattt atatatttaa taaaggttat gcgcgacttt
     6061 ttgatttttt catttgcgaa caaaaacaaa cagactttaa gagtaaaaag gattaaaaat
     6121 atacagaaat cacgaaataa aaaattaaca aaacaacaaa ttcaaacata atccaaacat
     6181 ctttttttca tcacacaaat cgattaaaac ggtgcgactt tttaatgata tatatatcta
     6241 tgaaatgaaa atgcgagcaa agttttttcg ttttcagttc atcatagaaa tactatataa
     6301 actccgtaat ttcggaatac cgagttacaa agaaaataga aaacaaaaat ttcgaaaatc
     6361 gagttttcaa attcaacaaa gttcaaaatc aaattccaca tcaaaatcta agattcctca
     6421 aaacctcccc caaaaataaa ccttcaaaaa acgtacggta ttttatcgtt atatatataa
     6481 ctaaaaaaat tcatgcgcac actttagttt tttcatcctg tacggtagta acaggaagtt
     6541 ttagggtata aactatatag cttataaatt taaaaaggaa ttcacaaatc cacgaaatta
     6601 aagttttaaa aaaagaaaga cattttgtct ttcttttttt aaaaccaatt accttttgtt
     6661 tctgacatcc gtttttttat ctctagcatt ttttcatttg ttctctttct aacggatcac
     6721 taatgtcatc taattctaat ttttttaacg gttgtacaaa tttatagctg ccattctttt
     6781 tttcagctaa ataataatac tgtaattcat cattccaata atatatgact tgttcactca
     6841 acatcaaagc cccctgtaat caccttaata tcagtccatt acaccatcat agcatcaata
     6901 aagacccaaa tttaacgcaa tacaaaaggg acagtcccaa aagtaaccgt atcaaaaagt
     6961 agtcccaaat aactgataaa cgcatcataa agccaaaaac aagtgtctca aaaagtccct
     7021 aaaatcattt gcaaacgttc ataaaaatat atataattta tgaacggtag ggttatatgt
     7081 tttatatata tataaaaccg cacccttttt gaggttattt ttctttgatg acaatcaata
     7141 taaaagtcat taaaatgaat aatatacggt atacatgcaa taatagtcgt agttattctt
     7201 aaaaataaaa ttaagttcta aatcataaaa aaatccttcc aagtgcgcca acactcgaaa
     7261 ggattaacca ttttttattt tccaacctta tttttaccat tttccagtag ttctgctaat
     7321 tgtccagtta gtagaacttt tttcttttca taatccccag caacatcaaa taacttcgga
     7381 acatattgtt ttaatttctt tctccaagta cttctttgaa atttatccat cataccaaaa
     7441 taacgaccat gataatcatt cataatagct tctaaaacta tacgatcacc taacttcatt
     7501 tcacttccat ccaaactaac taaatcacca acaattatag aatcaaacgg attataagtt
     7561 gactccatcc actcacttgc ttttttctta cgtaaacgag cttcaaaacg cgttacatgt
     7621 tctacatcag gatactgatc caacgtgtca ttctcctcat tttctcgctt cttatcgtaa
     7681 atacaaagat gattatctga agcacgtttc cccacataca tcgtttccaa ggatttatcc
     7741 ctatctagaa aaatacattc tgatttacta gataaatctt ttattttcaa ctctgcaatc
     7801 ttaattggat aatcacaagc tatatgtata cccgtaacct caacgttttc taacaaccct
     7861 aatacaagtc ttatagattc atcgacaaca cgattatatg ccatatgctt agggttaaac
     7921 tcaaacctaa catctttcaa caaacgatca tttttctttt tcccgttatt cggaagaaaa
     7981 ccatttgcat ctaactgtaa aaacatttca gcatttctta acagctcatc ttttttaact
     8041 tcaatcaatt tctctttatt ttccaaagtg ttaagtagta tctgaacatg ttcctcatga
     8101 attttttcca tcgcttcatt tttaaaaccc gttgcaagtt cttgctcatc tatttcagat
     8161 ttcaatgcga ttatttcgcc tttcaacttc tcaatagtaa gttctatttg tctctcatgc
     8221 cgtttcaatt tccgttcatc agtcaaacga ccatcttcat ccgtttcagc tatcaattct
     8281 ttcacatcag ccaaactttc aaacaaacct tgatactcag cattagaaag taacgattct
     8341 ccaacttctc ttgctttctt ctcctgctct atttgaattt ctaatctaat cctatgagaa
     8401 attaatttcg gaagatcaac tgacgctttt ttcaattctc ccgataactc aatataacca
     8461 caatcaacag agtcacattt caaaacttct tcatacccaa aatttttcga acggtttgcc
     8521 tgcaatgaat atggcatatt tgaacttgca accattctta taaactcttc ttttttaata
     8581 ccttctttaa aatctgcaac aatacttaat ttatcaatcg ttgcatccaa taaactggat
     8641 tctaatacgt cattcataac aaatccgcaa ccccccaaca gttgtatttt ttgtgtgctt
     8701 gtccaaggtg tttctgaaga aggtagagaa attctcaacc tttacctgct tattagaata
     8761 acaggtaact ttttttcgag cgttaatctc cctcaactaa aaaatcattc caaaacacat
     8821 ttacttccca ttttcttttt ccaaataaga aaaataaaac tcaaatgctt tttctgcaat
     8881 ttccgctttc gtagaaaaac gtccactact aaccaatccc ttaatccttt cagatgtttc
     8941 ttttggcaat ctaaaagtct cagggatttt cggattctta ccgtctttcg gtttcctacc
     9001 aacagtcaca taattagctt tactcattaa aacacctcca aataacccag taacaacatg
     9061 ttatcatttc gtatgacgtt ttgtaaataa ctttgtttta aaagaacata aaatccgaat
     9121 ataaaagaac tcataaagag ttctcccaac ctataattca tctccataaa ccaa
//
