LOCUS       NZ_CP083098             9463 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain B401 plasmid pP05_2-m, complete
            sequence.
ACCESSION   NZ_CP083098
VERSION     NZ_CP083098.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214334
            Assembly: GCF_020809125.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x, 60x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809125.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,136
            CDSs (total)                      :: 6,981
            Genes (coding)                    :: 6,638
            CDSs (with protein)               :: 6,638
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 343
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 343
            Pseudo Genes (frameshifted)       :: 120 of 343
            Pseudo Genes (incomplete)         :: 233 of 343
            Pseudo Genes (internal stop)      :: 92 of 343
            Pseudo Genes (multiple problems)  :: 78 of 343
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="B401"
                     /isolation_source="Biopesticide B401/B402 (Certan),
                     Vita-Swarm"
                     /db_xref="taxon:1428"
                     /plasmid="pP05_2-m"
     gene            complement(join(9192..9463,1..457))
                     /locus_tag="K8Z39_RS35350"
                     /old_locus_tag="K8Z39_35215"
     CDS             complement(join(9192..9463,1..457))
                     /locus_tag="K8Z39_RS35350"
                     /old_locus_tag="K8Z39_35215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021728966.1"
                     /translation="MKIKQISAVLSGALLIGSLSPSISQAATQKETPVKTEQVQEKQNN
                     QNNNFIPIQTNEQFNQTLNTTKTFTPYVKVNAEGLLYINATADEIGITNEEYQNFLNGL
                     NLENEYIKKGYFTLEKNEQGNPGKLSTTQALNSYVNNDLSQFKELQSSSAGFYGWGFGL
                     KLSNSETQSLAEAMAWGSAAIWVAAELAAAGYVTIPASVPLGLIAAIVGLGAATLAVVN
                     KGKGVTVGITWTGHPHIHRN"
                     /name="hypothetical protein"
     gene            complement(664..804)
                     /locus_tag="K8Z39_RS35355"
                     /old_locus_tag="K8Z39_35220"
     CDS             complement(664..804)
                     /locus_tag="K8Z39_RS35355"
                     /old_locus_tag="K8Z39_35220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023901786.1"
                     /translation="MKKTTFYLLGIITAFTLTLGNTNTSKETQISAEKNIIQYSHGNTG
                     G"
                     /name="hypothetical protein"
     gene            complement(801..1895)
                     /locus_tag="K8Z39_RS35360"
                     /old_locus_tag="K8Z39_35225"
     CDS             complement(801..1895)
                     /locus_tag="K8Z39_RS35360"
                     /old_locus_tag="K8Z39_35225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap family tetratricopeptide repeat protein"
                     /protein_id="WP_050062578.1"
                     /translation="MDVQTKNNEQITNMLNDWYIEIRARHLGNAHKLRLEIDKKIHNIE
                     EDQNLLLYYSLLDFRHQYLMDHLSIGKNSFDKIESFNTPTDNLLSYYYFFFKAIHATSI
                     GNYNLARKYYDKAEIKLKEIPDQLEHAEFYYKLSTFSCHNQQYVPAIKQASKAKEIFSK
                     YKGYELNIGYCNNLSGLSCTHLQEYELAEEYFISAMDIFQKEKEEQAILYVRHNLGFMY
                     AKQNLSELAIRYLSEVIQKSPNNYRAILIEACEHSKLNNKNEASELFEQGLQISTELEN
                     EEYQHHFKILKAINEEIPGENLEKLVIAGNKYFEQENIYEYVHEYNEKLALKFYREDNH
                     EKASKYFYLSSKANQKKQDKEALK"
                     /name="Rap family tetratricopeptide repeat protein"
     gene            2350..3006
                     /locus_tag="K8Z39_RS35365"
                     /old_locus_tag="K8Z39_35230"
     CDS             2350..3006
                     /locus_tag="K8Z39_RS35365"
                     /old_locus_tag="K8Z39_35230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729703.1"
                     /translation="MKKIVDFSEVEKNARKRDLEIERKKHENHYTKEQEEGLGQALETL
                     QNLTGAEYYVGRKRDPFAQVEFSQIIQANVRYLIKIGYLTNAERLLLLDLSCYVEVGTH
                     VLVEKDVDSIDRDVINNASVTYLAEETYRTRHGLSKLMKNLKDKGILACAESGFSDESG
                     RVCTKRTWLVNPNIICASRKTSIDRVTKHIFKGILKNFTLDSDSKKIHNLPVYFF"
                     /name="hypothetical protein"
     gene            4032..4670
                     /locus_tag="K8Z39_RS35370"
                     /old_locus_tag="K8Z39_35235"
     CDS             4032..4670
                     /locus_tag="K8Z39_RS35370"
                     /old_locus_tag="K8Z39_35235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001996445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729419.1"
                     /translation="MAITKRDIEIVEWVNLHGFVLAEQVQEFFGLGKTVVYRRLKEMVE
                     QGVLKRERILYDYGNVYWVTKDGVELCESEMAAGKKPSVNNFVHDKKLVDLSVKLLKKY
                     EGSSWITARQIKSRLVRKASEKDKFKALAMRVADGILIVNGKKYAVELELSLKNRTRVK
                     QIIGDYAERIAKGQYVSVIYVVEKESIAKVLREEMNQTVVSDRFQIMTF"
                     /name="hypothetical protein"
     gene            4733..6625
                     /locus_tag="K8Z39_RS35375"
                     /old_locus_tag="K8Z39_35240"
     CDS             4733..6625
                     /locus_tag="K8Z39_RS35375"
                     /old_locus_tag="K8Z39_35240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_021729418.1"
                     /translation="MATNQYEENRNSLEWIRFAAVICFTSGYLIAIIFKKIGELAQVGK
                     LEKRIVFWIAFGFSILWVLNDFQTLLASSVVSFVISFVENYFQVLPMELKHFVFAIFPE
                     HNTLLAFLVNGVKTAGLVIAIENVFKWATRVDESTVQNSPSLEREKQEKSLANKVIVNL
                     KEKQQEKSYQKEMDRGEIPKGVRLGRTMTGGQEIILTPDVLKKHIGLVGTTGSGKTITI
                     KTFLEYAILKKQAGIFIDAKGDMKLAEEIRKFAEKHKRPFYHFNTDGVGMHYNPLAVGT
                     PTELTDKILTLSDWSEEHYKLSSQEFLQYLFRVFEARNVKATLTSVSQYCDADALIGLI
                     TSQSEIEVHEEAPKKVVVSDLSDTDSLVLFDDEKIDVSKSDPSESIFKRDEVFNLIPKI
                     KRIDKKAIEGLASRVSILAEGDFRHLFQEEVGNTIALHQVLEERGMVIFTLDSLRYPEG
                     ARQLGKMIVNDLKTNVSSHMKHREGEHVTVAIDEFNVLVTQQVIDVINKSRGAGFEALL
                     AFQSLADIETVSEHLRKQIMQNCNTLIVQAQNDPSDAEELAKAIGTADSLDFTYQVDMA
                     QGVTGLGSMRNVKNFVFHPDEIKALQTGEALVKIKQGDKLVRKKIKVRMVKWED"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     gene            6656..7186
                     /locus_tag="K8Z39_RS35380"
                     /old_locus_tag="K8Z39_35245"
     CDS             6656..7186
                     /locus_tag="K8Z39_RS35380"
                     /old_locus_tag="K8Z39_35245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="WP_021729417.1"
                     /translation="MDYYIYAFSLIVTKGLLKTANEEELAGILAHELGHLKYGDSKRSV
                     MAHTLNTAGTVATWILVAMATILSLLSNRVPIVAVLAALAAVVLKAMQVVLHRVADLSF
                     RVVGRKEEFRADAYAVELGMGAGLLSFLEKIEGTEGSPKGIWARLEATHPPVAFRIEQI
                     EISLEEQQKETIL"
                     /name="M48 family metalloprotease"
     Typing          2351..3006
                     /name="rep_cluster_1767"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.043"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4734..6598
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.141"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaagattg taattctttg aattgactta ggtcattatt aacatatgaa tttagtgctt
       61 gtgttgtgga aagttttcca gggtttcctt gttcattttt ttctagagtg aaatatcctt
      121 ttttaatgta ttcattttct aagtttaatc catttaagaa gttttgatat tcctcatttg
      181 ttataccaat ttcatctgct gttgcattaa tatataaaag tccttctgca tttactttta
      241 catatggagt aaatgttttt gttgtgttaa gtgtttgatt aaattgttcg tttgtttgaa
      301 tgggaataaa gttattgttt tgattgtttt gtttttcttg tacttgttct gtttttactg
      361 gtgtttcttt ttgtgttgcg gcttgcgaaa tgctaggtga taaactaccg attaacaaag
      421 ctccacttaa aactgctgaa atttgtttta ttttcattat aaaaccacct tttttgtaat
      481 ttggaatata ttagattcct attacatttt atttcatatt tataaataat gtaatattat
      541 gttaaaaatt gtaatattat gcttatttat caaaaccttg aaacatgtga tttttttata
      601 aaaaaaacct tctgaagtag aaggcttttt tgtttgtatg cttaaagttt taaaatcaat
      661 tagttaacca ccagtgtttc catgtgaata ttgtattatg tttttttcag cggatatttg
      721 agtttctttt gatgtatttg tgtttcctaa ggttaaggtg aaagcagtta tgatccctag
      781 taagtaaaaa gttgtttttt tcattttagt gcctccttat cttgtttttt ttggttagct
      841 tttgaactta ggtaaaagta tttgcttgct ttttcatgat tatcttcacg ataaaatttt
      901 aatgctaatt tttcattata ttcgtgaaca tactcatata tattttcttg ttcgaaatat
      961 ttgttacctg ctattactag tttttctaaa ttttctccag ggatttcttc atttattgct
     1021 tttaaaattt tgaagtgatg ttgatattct tcattttcaa gttctgtact aatttgtagg
     1081 ccttgttcaa atagttctga tgcttcgttc ttattattta gttttgaatg ttcacatgct
     1141 tcaattaaga ttgctctgta gttatttggt gatttttgaa taacttcgga taaataacga
     1201 attgctaatt ctgataaatt ttgttttgca tacataaatc ctaggttatg acgaacatat
     1261 aaaatagctt gttcttcttt ttctttttgg aatatatcca ttgctgatat aaagtattct
     1321 tcagctaatt cgtattcttg tagatgtgtg catgataatc cagaaaggtt attacaataa
     1381 cctatgttta attcatatcc tttgtattta gaaaagattt cttttgcttt agatgcttgt
     1441 ttaatggctg gaacatattg ttgattatgg caactgaatg tcgaaagttt ataataaaat
     1501 tcagcatgtt ctaattggtc cggtatttct tttaatttta tttcagcttt gtcatagtat
     1561 tttcttgcta agttgtagtt tccgatgcta gttgcgtgta tggctttaaa aaagaaataa
     1621 tagtatgaga gaagattatc tgtaggtgtg ttaaaagatt caattttgtc gaagctattt
     1681 ttaccaatac ttaagtgatc cattagatat tgatggcgaa aatctaatag tgaatagtat
     1741 aaaagtagat tttggtcttc ttctatgttg tggatttttt tatcaatttc taaccttaat
     1801 ttatgtgcat tacctaaatg tctagcacga atttcgatat accagtcatt taacatgttt
     1861 gtgatttgtt cattattttt tgtttgaacg tccacaaagt agcccccttt ttacaaatgt
     1921 atattatttc cactcatttt atcatgtctc gacatatatt tactttatta aatttgatat
     1981 atattatttt taatattcag taaattacgg ttgaaaatta tacccattaa caaagaaacc
     2041 ttaagtatta aggtttcttt gttaatgggt ataaataagg tatttgtctt attgacattt
     2101 ttaggatatc aaaaatgtat gaatttgtat atatttcttt ttgtgaatat aaaaagtcag
     2161 acaagataca acaaaaaaag ccattcatgc tataattgag ttgcttagac aacaactata
     2221 gtgaaggggc tttttttgta tcttaaaaga ttattagagt atcagactca aagaaaataa
     2281 ttctaattac ataataacaa aataaaagtc tctttgctag ttgtaaaaac tagaagtgga
     2341 gagttattta tgaaaaaaat agtggatttt tcagaagttg agaaaaatgc gagaaaacgt
     2401 gatttagaga ttgaacggaa gaaacatgag aatcattata cgaaagagca agaagaagga
     2461 ttaggacaag ctcttgaaac attacaaaat cttacgggtg ctgagtatta tgtaggacgt
     2521 aaacgagatc catttgctca ggtggaattt tcccaaatca ttcaagctaa tgtacgatat
     2581 ttaattaaga ttggatattt aactaatgct gaacgtttgt tacttttaga tttgagttgt
     2641 tatgtagagg ttggaacgca tgttttagtt gaaaaagatg ttgatagtat tgatagagat
     2701 gtaattaata atgcttcggt tacttatctt gctgaggaga cttatagaac taggcatggc
     2761 ttaagtaagt tgatgaagaa tttaaaagat aaaggaatat tagcttgtgc cgaaagtggc
     2821 ttttctgatg aatcaggaag agtttgtaca aaacgaactt ggttagtaaa tcctaatatt
     2881 atttgtgctt caagaaaaac atcaattgat agagttacga aacatatttt taaaggtatt
     2941 ttgaagaatt tcacattaga ttcagattct aaaaaaatac ataatttacc ggtttatttt
     3001 ttttgagaga tactctagta tttctctttt ttttatgtta gaaccgaaca aactttcgta
     3061 ctaaaatttg tacactagtg tgcaaaaatt tgtacactcc ccccctcatg aacccttgat
     3121 aacactgact tttttttttt tccttcttac tcttatatat atatgctctt tcgttccata
     3181 tttttgttgt tttttgaggt taagatacgt tatatttcca cgctatattt agataaaaat
     3241 ccgaaggatt gccccttttt gtagtttccg tgcttcgcta tcgtactggc tagccaaaat
     3301 aggctatcat ggggattctc attgtaaagg gtttggctac gcccgttccg ctatgctaca
     3361 cgccctttac aatgagcctc caccccatga cttatttgcc agtgcgccag ctctggccgc
     3421 ttagcatggg aaactacaag gggcgttcct gattaaataa tagatttcaa aacgataaaa
     3481 ataaaaataa caaaaccttt tgaaaatcac aaaatataaa gattgaaaaa ttataaaaga
     3541 aacaaacatt tgatttcatc aaacgtttgt ttaaaaatag ccgcgctatg gcgcggctat
     3601 ttgcaaagac ctatgaaaaa acatatagaa aaaattatga tatatagttc catacttatt
     3661 ttccatagtt tgcatgtaga ctgaagttgc atttagacgc tgtatatagc cgattccccg
     3721 cccctctcct tcttttcttc gctattgcta cgaacctcct gaccactcac atactacgtc
     3781 tacatgtgag tggtcaggag gtcagaagga gaggggcggg gaatatgaga attgagtaag
     3841 agaaatagag tagaagaata agagagaaag agagaagaat aagtgtaaaa tagaaagaag
     3901 ggatagacga atagtaagta tggaaataga tatggaagac gatggtaaga atatggtaaa
     3961 ataataggtg gtaaagataa gagattggaa gagtagatgt atagaaggat agaagagtag
     4021 gagagggaaa tatggcaatt acgaagagag atatagagat agtagagtgg gtaaatttgc
     4081 atgggtttgt attggcagaa caggtacagg agttttttgg tttgggaaag acggttgttt
     4141 ataggagatt aaaagagatg gtagaacagg gggtattgaa gagggagagg attttatatg
     4201 attatggaaa tgtgtattgg gtaacgaaag atggtgtgga attgtgtgag agtgagatgg
     4261 cagcggggaa aaagccgagt gtaaacaatt ttgtgcatga taagaaatta gtggatttgt
     4321 cggtaaagtt gttaaaaaaa tatgagggat caagttggat aacagcaaga cagattaagt
     4381 ctagattagt tcggaaggca agtgaaaaag ataagtttaa agcgttagct atgagagtag
     4441 cagatggaat attgattgtg aatggaaaga agtatgcagt ggagttagag ttgtcattga
     4501 agaatcgaac aagagtgaaa cagattatag gagattatgc agagagaatt gcaaaagggc
     4561 agtatgtgag tgtgatttat gttgtagaga aagaaagcat tgcaaaagta ttacgggaag
     4621 aaatgaatca aacagttgta tcagatagat ttcaaattat gacattttaa tttaggttaa
     4681 ccttagttaa ctaggttatt taaggttaac ttttaaagag aggaatataa atatggcaac
     4741 taatcagtat gaagaaaatc gaaattcatt agaatggatt cgttttgcag cagttatatg
     4801 ttttacaagt ggttacctta ttgcaattat ttttaaaaag ataggtgaat tagcacaagt
     4861 agggaaatta gagaaacgaa ttgtattttg gattgcattt ggattttcga ttttatgggt
     4921 attgaatgat tttcagacat tgttagcatc tagtgttgtg tcatttgtga tttcatttgt
     4981 agaaaattat tttcaagtat tgccaatgga actgaagcat tttgtatttg ctatttttcc
     5041 agagcataat acgttgttag cttttttagt gaatggtgtt aaaacagcgg ggttagtaat
     5101 tgcgattgaa aatgtgttta aatgggcaac tagagttgat gaatcaactg tacaaaatag
     5161 tccctcttta gagcgagaga aacaagaaaa gagtttggcc aataaggtta ttgtgaattt
     5221 aaaagaaaaa caacaagaaa aaagctatca aaaagaaatg gatcgtgggg aaattccaaa
     5281 aggtgtacgt ttaggaagaa ctatgactgg cggtcaagaa atcatactaa caccagatgt
     5341 tttaaagaaa catattggat tagttggaac aactggctcg ggtaaaacga taacgattaa
     5401 aacatttttg gaatatgcaa ttttaaagaa gcaagcaggg atttttattg atgcaaaagg
     5461 agatatgaaa cttgcggaag aaattcggaa gtttgctgaa aagcataaac gtccttttta
     5521 tcatttcaat acagatggag ttgggatgca ctataatcct ttggctgtag ggactccaac
     5581 agaattaaca gataaaattt taacgttgtc tgattggtct gaagagcatt ataagttatc
     5641 ttctcaggaa tttttacaat atttgttccg tgtttttgaa gctaggaatg taaaagcaac
     5701 tttaacaagc gtctctcaat attgtgatgc tgatgcttta attggattaa ttacaagcca
     5761 atcagaaata gaagtacatg aagaagcgcc taaaaaggtt gttgttagcg atttaagtga
     5821 tacagatagt ttagtgttgt ttgatgatga aaaaattgat gtaagcaaat ctgatccttc
     5881 tgagagtatt ttcaaacgtg atgaagtttt caatttgatt ccaaaaatta agcggattga
     5941 taaaaaggca attgaaggtt tggccagtcg tgtttctatt ttggctgaag gagattttcg
     6001 acatttgttc caggaagaag tgggaaatac aatcgcatta catcaagtgt tagaagaaag
     6061 agggatggtt atttttacat tggatagttt gcgatatccg gaaggcgctc gacaacttgg
     6121 aaaaatgatt gtgaatgatt taaaaacgaa tgtgagtagt catatgaaac acagagaagg
     6181 tgaacatgta acagttgcta ttgatgaatt taatgtactt gttacgcagc aagttattga
     6241 tgtcattaat aaatctcgtg gtgcaggatt tgaggcattg cttgcttttc aatccttagc
     6301 agatatagaa acagtgagtg agcatttacg aaaacaaatc atgcaaaatt gtaatacttt
     6361 aattgttcaa gcacagaatg atccaagtga tgcagaagaa ttggcaaagg caattggtac
     6421 agctgatagt ttagatttta cttatcaagt ggatatggct cagggcgtaa cgggattggg
     6481 aagtatgcga aatgtgaaaa attttgtgtt ccatccagat gaaattaaag cattacagac
     6541 tggggaagct ttggtgaaaa taaagcaagg tgataaacta gtaaggaaaa aaattaaggt
     6601 aagaatggta aaatgggagg attaattacg tgttaatgtt aacagctttt gtgaattgga
     6661 ttattatata tatgctttta gccttattgt gacaaaggga ttattaaaaa cagcaaatga
     6721 ggaagagctt gcagggattc ttgctcatga acttggtcat ttgaaatatg gggatagtaa
     6781 gcgttctgtc atggcgcata cgttgaatac agcgggtaca gtagctacat ggatattagt
     6841 ggctatggca acgattttat ctcttctctc gaatcgtgtt ccgattgtgg ctgtattagc
     6901 ggcgcttgca gcggttgtat taaaagccat gcaagtcgtt ttacatcgag ttgctgattt
     6961 atcgtttcga gttgttggaa gaaaagaaga atttcgagcg gacgcgtatg cagttgaatt
     7021 aggaatggga gcaggtttgt tgtccttttt agaaaaaata gaaggaactg aaggttctcc
     7081 aaaagggatc tgggcgcgtt tagaagcgac tcatccacct gtagcatttc gaattgaaca
     7141 gatagagatt tcgttagaag aacaacagaa agaaacgata ttataaagga atttatgata
     7201 taataaatat tcatcactaa ctaactttaa gggcttcatc cttttaaaaa tctctgtata
     7261 tgtgtgcata tacagagatt ttttgcgttt atgtacttgt aagtatggta agattgataa
     7321 gactacattt gaaaatgtgg gttcaagttg ctgtttgact gaattgcacc aaaaaaccaa
     7381 ttccattaca cgggaattgg ttttttgggt gaaatttagt atatttattg ctagcctaaa
     7441 ggagattcgt ttctttctaa tttagtataa cttaaataga aagaaaattc aaaaataaga
     7501 gaatgaatat ttacatattt tcaaattaaa caacaaaaat gtaaggatat gggttataat
     7561 gagttaagaa acattctcct gttcctatgt ttctattgat tgttattttt atctgttttt
     7621 ggtgcaaagt ctgtagatag attttgcacc ccccttcatt ttttatattt atatacggat
     7681 attaagattt tgtgtataat gattatgtct gtagtttctt ttaattcgtt tatataaagg
     7741 gcaattcctg tttttttaag gaattgccct tttgtgtgcc ttttataaat caatacagta
     7801 tatgtgacaa tagatgagaa ggttcttatt atatggaaat atacaagtta atacaaaaag
     7861 aaatagcatg ttttcttttt taaaaaacaa agattctttt atattttgtt tattttcaat
     7921 gagacatact caaattttat aataagagca tatttttata aagtatcttc ttcttttgaa
     7981 cagaatagaa gaatgcaaga gatgactttt tgctggatat aagtgataca gatagtttgt
     8041 atatttgtat cacttatcca tttaaaaata aaagaataaa ttaatacata attttgaatt
     8101 aatacaaaaa aatgtaaaaa cacagatggt aggtagtaaa atccttttct atgtttctgt
     8161 tgataaaatt ccacttttag aatcattaga caaaagtgtt aatatatagg ttataataaa
     8221 gaggtaataa gttcagtttt ctgagtttaa tatgtgtatt taaatgagcg atcggttttg
     8281 tttgttttgt aggaaaatga gaagtctttt taaaaagact tctcattttt atggatggaa
     8341 ggagtgatat atattataaa ctattattct tatcatcaaa tgtttttaat ttttatgacg
     8401 gttatagcta tatttttata tattctatat gtgaacagac cgacttcaaa aaataaatgg
     8461 aatcttttga tcgtagtgtt tttatttttt tttattaatt atttatagtt tttttgtagt
     8521 aaagctaaag taaactttta aatattagtt tactttcaaa gttttgattt tataggctcg
     8581 taagaggttt ttagaaaaat agatatttcc gtaaggaaag tatacattca gaacacgaga
     8641 atgaaaggat ttccacgccc gacctttgcc ccctagtctt cgcagtacgg agtggcgcac
     8701 tggcatagga aacacaggtt ggaggctcga tgtaaagggc atgaagcgta gcggaatggg
     8761 catagccgaa ccctttatat ggagaccgcc tgtgttagcc tattcgggca agccagtccg
     8821 gtttgcgaag tgggggtaaa gagaaggagg gcataaagtg gataaaaaac aaaaaatcta
     8881 agcttgtaag cttagatttt ttgtttttta tgtagcattt aataataaga aaagccatgt
     8941 aatgaaaaat gctacaacta agggaattat ttttaaaaag attaataggt ttttatttaa
     9001 gtaggtgtat ttttttttgt tgaaaatata ttctaggcta actgttatac aaataaggat
     9061 cagtataatt aaaatagatt tcttgaacag taaattagat accaaattat ataaactaag
     9121 acctaagagt acgattatag atgcaaataa tcgaatgttt tttttcataa atcgttctcc
     9181 tttttcattt attagttacg gtgtatgtgt ggatgtcctg tccatgtaat tccaactgtg
     9241 acgccttttc ctttgttaac aacagctaat gtagcagctc caagtccaac tatagctgca
     9301 attaatccca atggtactga agctggtatt gttacatatc cagctgctgc taattcggct
     9361 gcaacccaaa ttgctgcact accccatgcc attgcttcag ctagtgattg agtttcagaa
     9421 ttacttagtt ttaatccgaa tccccatcca taaaaaccag ctg
//
