LOCUS       NZ_CP083097            10657 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain B401 plasmid pP05_2-l, complete
            sequence.
ACCESSION   NZ_CP083097
VERSION     NZ_CP083097.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214334
            Assembly: GCF_020809125.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x, 60x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809125.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,136
            CDSs (total)                      :: 6,981
            Genes (coding)                    :: 6,638
            CDSs (with protein)               :: 6,638
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 343
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 343
            Pseudo Genes (frameshifted)       :: 120 of 343
            Pseudo Genes (incomplete)         :: 233 of 343
            Pseudo Genes (internal stop)      :: 92 of 343
            Pseudo Genes (multiple problems)  :: 78 of 343
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083097.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="B401"
                     /isolation_source="Biopesticide B401/B402 (Certan),
                     Vita-Swarm"
                     /db_xref="taxon:1428"
                     /plasmid="pP05_2-l"
     gene            506..1780
                     /gene="ltrA"
                     /locus_tag="K8Z39_RS35295"
                     /old_locus_tag="K8Z39_35160"
     CDS             506..1780
                     /gene="ltrA"
                     /locus_tag="K8Z39_RS35295"
                     /old_locus_tag="K8Z39_35160"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421374.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_021727498.1"
                     /translation="MELLEAILSNKNMNEAYLRVYKNKGVSGVDGVTVEELKQYLKEHK
                     DELRQRIRTRKYQPQAALRVEIPKENGKMRKLGIPTVVDRVVQQAIHQILSPIFEKQFS
                     EFSYGFRPKRSCEMAIVKSLEFLNNGYDWIVDIDLERFFDTVHHDKLMRIISNSIHDGD
                     VISLIRKYLVSGVMVNGKYEETPVGTPQGGNLSPLLSNIMLNELDKELEGRGLQFVRYA
                     DDALIFVRSEKAAGRVMKSIVRFIEKKLGLIVNTEKSKISRPKDLKFLGFGYYYTLKDK
                     RYQTKPHPISVQKFQRKLHQLTKRSWSVPLDYRILKLKQVIFGWVNYFRSTNMKGVLER
                     VDQKLRARIRVIIWKQWKVPKKQIKSLVQLGIPEEEAKGLTYCRKGFRYIGLSKVVQRA
                     ISNQRLKKRGFPSSLERYLKVHTVI"
                     /name="ltrA"
     gene            complement(1861..2262)
                     /locus_tag="K8Z39_RS35300"
                     /old_locus_tag="K8Z39_35165"
     CDS             complement(1861..2262)
                     /locus_tag="K8Z39_RS35300"
                     /old_locus_tag="K8Z39_35165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_021727499.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKISEQEASD
                     FTVNVVEQRLSFDEILNVIKENSSKLPSN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(2300..2584)
                     /locus_tag="K8Z39_RS35305"
                     /old_locus_tag="K8Z39_35170"
     CDS             complement(2300..2584)
                     /locus_tag="K8Z39_RS35305"
                     /old_locus_tag="K8Z39_35170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_021727500.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEGLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            3172..3744
                     /locus_tag="K8Z39_RS35310"
                     /old_locus_tag="K8Z39_35175"
     CDS             3172..3744
                     /locus_tag="K8Z39_RS35310"
                     /old_locus_tag="K8Z39_35175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097509.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_011279306.1"
                     /translation="MSKKEHLIDVQPIRSKEQLQDMKWALKRHCTDRDYILFVIGINTG
                     LRVSDLLNLETSMILELKKKRRKELKIREGKTKKERMVNITSIFEEVYLYAAGLESKWL
                     FPSRKGDRPISKIQVYRQLQKAGDFAGVESIGTHTMRKTFGYWFYKQTKDVAMLQEILN
                     HSAPKITLKYIGINKEEKDNVLDTFCL"
                     /name="tyrosine-type recombinase/integrase"
     gene            4514..5557
                     /locus_tag="K8Z39_RS35315"
                     /old_locus_tag="K8Z39_35180"
     CDS             4514..5557
                     /locus_tag="K8Z39_RS35315"
                     /old_locus_tag="K8Z39_35180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_021727501.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIRIYEFIEKKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKGEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKSYFKKKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYAG
                     LLKDYAKKYENGELDKYKSIDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGHL
                     IDEMEVE"
                     /name="protein rep"
     gene            5560..5727
                     /locus_tag="K8Z39_RS35320"
                     /old_locus_tag="K8Z39_35185"
     CDS             5560..5727
                     /locus_tag="K8Z39_RS35320"
                     /old_locus_tag="K8Z39_35185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011279307.1"
                     /translation="MYKKGDIVKHTCWECYNEEMKIYRISKSKVKDGEDVYYSYKCWVK
                     CEKCGAVDDV"
                     /name="hypothetical protein"
     gene            complement(6587..6850)
                     /locus_tag="K8Z39_RS35325"
                     /old_locus_tag="K8Z39_35190"
     CDS             complement(6587..6850)
                     /locus_tag="K8Z39_RS35325"
                     /old_locus_tag="K8Z39_35190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874968.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPSELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(6864..7151)
                     /locus_tag="K8Z39_RS35330"
                     /old_locus_tag="K8Z39_35195"
     CDS             complement(6864..7151)
                     /locus_tag="K8Z39_RS35330"
                     /old_locus_tag="K8Z39_35195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000332102.1"
                     /translation="MAYVISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            7510..7737
                     /locus_tag="K8Z39_RS35335"
                     /old_locus_tag="K8Z39_35200"
     CDS             7510..7737
                     /locus_tag="K8Z39_RS35335"
                     /old_locus_tag="K8Z39_35200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            9059..9628
                     /locus_tag="K8Z39_RS35340"
                     /old_locus_tag="K8Z39_35205"
     CDS             9059..9628
                     /locus_tag="K8Z39_RS35340"
                     /old_locus_tag="K8Z39_35205"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_021727495.1"
                     /translation="MKVFEVLESLKKGEKLKEIANVLGINNSTLQRKLKALGYAFDSST
                     KEWFYNDKDNPEPLEENIMDMVKRNSKSGVNDLPKIIQGIKSETKGELGVKHGNTVKVS
                     EVLTSEDIADLHEMLLFWRTDRERTKKHKTLHEEIKTLRRDDRTRKTVILSKEVAKRLD
                     KLSERERFDKSDLLELALEHFLSEYE"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            complement(9981..10565)
                     /locus_tag="K8Z39_RS35345"
                     /old_locus_tag="K8Z39_35210"
     CDS             complement(9981..10565)
                     /locus_tag="K8Z39_RS35345"
                     /old_locus_tag="K8Z39_35210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021727497.1"
                     /translation="MKNRYEHLKNGYTAIFFKDSNNKETAFFIDTDCFEIANSHDFYWE
                     NYLVNSQGKRMRYNIIGRKKGESSKTQLSRLLLECNTQKEACYNITGNPLDLRKCNLLK
                     TNMGKVKTKEFQEEMNKLIKTVPPLPQIQLKNINEKEKDVAISPKNDNIRLLTDEQNNT
                     YILLENGIITAEIEKKYAKFLVKQLSHSITT"
                     /name="hypothetical protein"
     Typing          4391..5557
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.377"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttatgtgtg ccaggcatgg caactatcta ggtggtggaa gtccactgtg ggggtacaca
       61 tcgaccaacc actaaggaag cgcaaggtac ttaccgtgag gtaagggctg gaggaagcgt
      121 ggaataaaat cttggctcga cgaacagaaa cctgatatga aggctttata aagggatgag
      181 gctccataac aagttaaagt ccaaaagatg tgcgtaactt tgtagagtaa atcaggcgag
      241 taaaagagga aagatagctg ccttaccctg ggagatcttg cggatgtact aacagtacag
      301 tcgaaaatgg ttaaccgcaa gaagtcagcc gaagccatag tagtggaaca aattcatgaa
      361 gggccgaaca gtttatagtg tttcaacgcc atgaatgcgt aagtgacgta ctccgaatat
      421 gttaatgatg aaagtatgag cgtatctcaa aggataatca aaatggagtt gtcacttact
      481 acgtgaaggg aaaggagaaa cgagtgtgga actcttagaa gcaattttaa gcaacaaaaa
      541 tatgaatgaa gcctacttac gcgtttataa aaacaaaggt gttagtggag ttgatggtgt
      601 aacagtcgaa gaactaaagc aatatctgaa agagcacaag gacgagctac gtcagcgcat
      661 tagaacaagg aaataccaac cacaagctgc cttaagagtg gaaatcccaa aagagaatgg
      721 caagatgcgc aagctgggaa taccaacagt agtggataga gtcgttcaac aagccattca
      781 tcaaatactc agcccgatat ttgagaagca gttcagtgaa ttcagttatg gttttagacc
      841 aaaaagaagt tgtgagatgg caattgttaa aagcttggaa tttctaaaca acggatatga
      901 ttggatagta gatattgatt tggagagatt ctttgacaca gttcatcacg ataaattgat
      961 gcgaattata tctaactcaa tacatgacgg agacgtcatt tctctcataa gaaagtatct
     1021 agtcagtgga gttatggtga atgggaaata tgaagaaaca ccagtcggaa ctccgcaagg
     1081 aggcaatctc agccctttac tgagtaacat tatgttgaac gaactggata aggaactcga
     1141 gggtagagga cttcaatttg tgagatacgc tgatgacgct cttatctttg tgaggagtga
     1201 gaaggcagcg ggcagagtga tgaaatcaat cgtgagattc atagaaaaga aactaggctt
     1261 gatagtcaat acagaaaaga gtaaaatctc tcggccaaaa gatttaaaat ttttagggtt
     1321 tggatattat tacaccctca aggataagag atatcaaacg aaaccacatc caatctcggt
     1381 acaaaaattt caaagaaaac tgcaccaact gacaaaacga agctggagtg taccgttaga
     1441 ctatcgaata ttgaaactta aacaagtgat atttggatgg gtcaattact tcaggagtac
     1501 aaatatgaaa ggtgttctgg agcgagtaga ccagaagctc cgcgccagaa ttagagtgat
     1561 tatctggaag caatggaaag taccgaagaa acaaatcaaa tcgcttgttc aactagggat
     1621 tccggaggaa gaagcgaagg gattaaccta ctgtcgaaag ggcttccgat atatagggtt
     1681 atcgaaagtt gttcaaagag ccatctcaaa tcaaagacta aagaagaggg gattcccttc
     1741 ttctttagaa cgctatctaa aagttcacac tgtaatataa actgaaccgc cgtatacgga
     1801 accgtatgta cggtggtgtg agaggggcga gaataattac tttattttcc ctctactcga
     1861 ttaatttgaa ggtaattttg atgagttttc ttttatgaca tttaaaattt cgtcaaaaga
     1921 taatcgctgt tctaccacgt taacagtgaa gtctgaagct tcttgttcag atatttttaa
     1981 ttcgtactca ttgtataaca gaaacatttc taggcaagcc aatgccgttc ttttgtttgc
     2041 attatagaat gcatggtttt tagccagtga ttcaaataat gcagtggctt tgtggaagat
     2101 agtcggatat gcatcctctt gtgctactgt ttgttgtgga cggtatattg ctgattgtaa
     2161 taaccccgta tctttaacac ctctttgttc gttaggtgaa tacatatcga taagtgtttc
     2221 attgataaaa atagcttgag tttctgttat ataaattgtc atttcggttt tcctactttc
     2281 ctccacgcct gaaggataat tatctgttta ccaaatcttt aaatgcaccg tcaaatttat
     2341 tgatggttct gtttaaggat tccaagaact taggattgag accctctggc atagaaattt
     2401 ttttggattt ttttataacg atttgttcgt tttcttcgtt aatttctaaa cttacaacgt
     2461 caccttgttt gatatttaga gtagtgtaaa attcttgcgg taatgttaaa cctaaagaat
     2521 tgccgatttt agtaacttta cgttcgattt tttgtgtttc catgtggcga gtgtttgctg
     2581 tcatttggac ttcctccttg gaatttgcca cactattttt tggcgtagtc taattgccac
     2641 tttgtgtttg tattgagttt ttcgaattat atagtgtgat tttatgtttg ttttgtttgt
     2701 ttgctttggg ttttttcggt ctttttagtt ttctcttttg tttgttgtgt ttaatctctc
     2761 tgtattatta tgacgtttag ttagaaagtt ataacagtta gaacgtatta accgttaata
     2821 caattatact catttttaga aatatgtaaa caaatttata tttcggtgca aaagataggg
     2881 aagattgtag agccttgtat aacgtcctcg ctacttgtcg ctgacaaaag agggggaaga
     2941 caaggtgaat tcgtttggaa gggcatagcg atacgctatg ccctcgctag tggacgttag
     3001 ttgtatttgt acatgtctct tttgcgttaa atgttgaatg taaactttta cacattagtt
     3061 tacattcaaa aatacgaaat cattgacttt acatcaaaat tgctgatact ctcaacatat
     3121 acaacaaaaa gtatacattc aaaacactgt aaataaagag ggtatgggct gatgagtaaa
     3181 aaagagcatt tgattgatgt acaaccgatt cgaagcaagg agcagttgca agatatgaaa
     3241 tgggcgctca aacgtcattg cactgatcgt gattatattt tattcgtgat tggaatcaat
     3301 acgggtcttc gtgtgagtga cttattgaat ttagaaacat ctatgatttt ggaattgaag
     3361 aagaaacgta gaaaagaatt gaaaattagg gaaggcaaaa cgaaaaagga acgaatggtg
     3421 aacattacat ctatttttga agaggtttat ttgtatgcag caggattgga aagtaaatgg
     3481 ctttttcctt ctcgcaaagg agacaggcct ataagtaaaa tccaagtgta ccgacagttg
     3541 caaaaggcag gagatttcgc aggagtggaa tctatcggta ctcataccat gagaaagact
     3601 ttcggctatt ggttctataa acaaacaaaa gatgttgcta tgttgcaaga aattttgaat
     3661 catagcgcac ctaaaataac gctcaaatac attggtatca acaaagagga aaaagacaat
     3721 gtgcttgata cgttttgttt gtaaaaaaga gtagaggtgc agagaaagtt acctgttact
     3781 ctaataagca ggtaaaggtt gagaatttct cacacttctt taaaaagcat ctcggacaag
     3841 cacacaaaaa tatctgtttt ggggttaaga atttgctatg aatgatgacg tcacaacccc
     3901 cctatgtaat acgggttgtg tgaccccaaa aataaagggg ttaaatctat cgaaaataca
     3961 ggaacatcaa gggttttcag gtcttttatt tgtgtaaaaa aagtgtttta aaaaaatgag
     4021 aaaaaaatac actttaaaat ttatggagaa aaccatgaaa gaaaggatac taattgacga
     4081 gtttatttct ttgttacgct atcatcatgt attccttaag gggaaaatat tataaatctc
     4141 ttaaaaacgt ttagaagtga aaatgaagcg tttgggagat agaagaattt ttgtgtttga
     4201 aagaaaaaaa tgcccttgtc cacgaagtgg attgaggggc atttttttat gatttttcgg
     4261 acacgaaaaa tacggttttc ttctttcttt aatacatagg taactatttc ggcaacgaaa
     4321 ttgatgccta tgtatggttt tatcccttga tacacaaggg atttctaaaa aaaaagactt
     4381 gcattcctag gtggatagat ttatcatttt tagtgtcgaa ttaaaaacaa taactaccaa
     4441 gaaaggagca agtccgaaaa agggattcgt ataccctttt ttaagcaatt cagataggac
     4501 taatatatca aacatgacaa agaaaaatca agatgaaatc actggtaaat tacaacctaa
     4561 aaagaaacag aatataagaa tttatgaatt tatagagaag aaaatgagtg agagcgggag
     4621 agaattgttt aaatcatgta gtacttttaa tgagtttgtg gctacgaaag ataaaaagaa
     4681 aaagaaacga gtaaagggga atgattgcaa aaatcgtttt tgtccaattt gcgcttggag
     4741 aaaagcagga aaggacgcag tgaaaatcgc aacgatgatg gaagcaatca agattgaaga
     4801 aaaaaaagag tttcttttcc taacattaac aaccccaaat attaaagctg atatggtaaa
     4861 aggcgagatt gatagattta ataaagcttt taaaaagtta tttgatagac gtaatattca
     4921 acgctctatt aagggttata ttagaaagct agaaatgacg tatgataaag aacgatttat
     4981 cactgaagaa atgtataaca ataaaaagag aaaagcatat tatgattctc gtggcttgaa
     5041 agtagatgat cataacccga attatgatac atataatcct cattttcacg tattactttg
     5101 tgttgataaa agctatttta agaaaaaaga actttatatt aaacaggaag aatggttaga
     5161 aatgtggcga gaagtaacag atatgccaga gattacgcaa gtacatattc aaaaagtaga
     5221 gttaatcaga gaaggaaatg cagttgcgga ggttgcgaag tattcagcaa aagattatga
     5281 aatgtcagtg agtcaagatg tgtttgacgt gttttattta gctttaaaag gtcgacaatt
     5341 aattgtgtac gctggattgt taaaagatta tgcgaaaaaa tacgaaaatg gcgaattgga
     5401 caagtataag agtatagata aaaatgagta ttattataga ttaattgcaa tgtggaataa
     5461 ggatttgatg aagtttgaac aagaatatca agagttaacg gaaagtgaga aacaagaata
     5521 taacggtcat ttgattgatg aaatggaggt tgaatagttt tgtataaaaa aggtgatatt
     5581 gttaaacata cctgttggga atgttataac gaagaaatga aaatttatcg gatttcaaaa
     5641 agtaaggtca aagatggtga agatgtatat tactcatata aatgttgggt taaatgtgaa
     5701 aaatgtggtg cggttgatga tgtttgaatg acaaagagag aaccaggctt aatattaagt
     5761 ttggttctct ctttgttgta ttgaaagaaa accttcttag tgtagcaagt agcattcaaa
     5821 agttttttta tgatctaact gagaactaac aagaaagatt ttttcttaaa tttaaatttc
     5881 gtgaatttgt gagtttattt ttaaagttta tttcgagata tattttgttc tgatgatttc
     5941 gttttactac tgtacgaggt gaaaacctaa ggtgtgcgca tgaatttttt tagatatatt
     6001 tatatcgata aaataccgca cgttttttaa tatttttttg aaaataagtt ttgggaaatt
     6061 ctaaattttt tttgaggttg attttggact tttgatatga gatttgatgt tgaaatttat
     6121 taaatttgaa atctcgattt ttcgattttc gaaatttttg ttttccgttt tttctgtaat
     6181 tctgtactcc aaaattacgg agtttatata gtacctccat gacgaactga aaacgaaaaa
     6241 actttgctcg catattcaat ctatagatac tttgtatctt taaaaagttg caccgtttta
     6301 aatgagttat tatagatcgt gtgatgaaaa aaagatgttt ggattatatt tgaatttgtt
     6361 atttccgtga attttttatt tcgtgattcc tgtatatttt taatcctttt tactcttaaa
     6421 gtctgtttgc tttggttcgc aaatgaaaaa atcaaaaagt cacgcataac ttttattaaa
     6481 tatataaata tattattaaa acacctaacc cttttgtgat gaaaaaatat aaatagttaa
     6541 agaaaaaaga ctgacaaaaa tatcagtctt tttttcttgt ttttagttat agagagtatc
     6601 tttttatggt attttctact ttttctctcc agttttctat atcgtcaatt gatattccaa
     6661 tctttattaa ttcgcttgga atgaaactgt tatctaaaat ccataaagtg agcctttcta
     6721 atgtgattct ttcgtttcca atcgaataaa tacgttcact ttgtttgcag gtatatggtt
     6781 ttttaccaac atatttttct acataaaagt atctttttcc gtttgctttg taatttgtta
     6841 gaaatgccat gttaatgctc ctttcaggta tttttatggt tagttaaaga gtagttctgt
     6901 aatgtttttt caactttttc ataccaatat ttcacatcgt ttatggatac tccgatttct
     6961 agtagttctt ttggtatacg gttgttatct aatagccaca ttgcgattct ttctaaagct
     7021 attttttgat tgccgattgc gtaaatgcat ttatatttgt attgctttga gtaatatggt
     7081 tgtgttccaa cgtattgagc tacataaaag taaatgtttc cttttacttt ttttgatatt
     7141 acatacgcca aaataatgac ctccaattat taaaattcta tatcacattg attaatatta
     7201 actttattag gttaattttt caacaaataa agtatgaaat aatgcatatt ttgaacattt
     7261 ttatttaaat aggttttaaa ttagtttaaa tgaacgcaat aaataacctt atatgtatag
     7321 aaattaattt tcgtttgaat gaagtatgaa ataatgcata atgtgatttt ttattccata
     7381 caggtttaaa aatgttttat aaagtgctaa agttgtgttt aaaaggttaa aaaacaacta
     7441 ttatttgcac aaaagtgcct ttataagaaa aatagtatgt atatttcatg agaaaaagga
     7501 gtataaaaaa tgaaagtagt aaataacttc cgtgtgatta tggcggtaaa gaatatagat
     7561 aatgttgcag agttggttag gatgacaggg gtaagtcgta actctatcaa taagttgtgg
     7621 cgtaatgata atgtgtcgtc tttaagaata gatacattgt taccgatctg tgaaaagtta
     7681 aatgttaaac tatcggattt aattgaatat attccagatg atagtgagac aaaataattt
     7741 aggggctatt ctatctttag atagcttgtg tatgatgaaa tcggtattaa tatttgctag
     7801 tggaaaacag gttatattcg catatggttt ttggtagggt tatatgcgta ttttaaattt
     7861 cggcaaactt ttatgactgt ctttttctgt ttgttatcac atgaatacgg tcaatgaaat
     7921 tataggcttg ctaggtgttg acgcatcttg gaagatgaga ggtaggcaga tacttcgtga
     7981 ttttgttgaa tcttatgtgc aatcatcgta atgcagtaaa gtgaaatggt ggaattgatg
     8041 agtattgaaa aaagagtccc tatttatggg gctctttttt tatttgttac taattacttt
     8101 taaaacgtcc acaaaacgta ctacaaaggt ctcaaagaga cctacaaata gatgttaaaa
     8161 ggactcaaaa cgacctacaa gggtgttttt tgaatcccag ttagagagct ttagaaattc
     8221 agtaggcaca gcctatttca ccactttgaa ctagaagtgg gatcggattt ccttatgctc
     8281 ttttttattg gaatgggtct aggaatgaat atagaaatag ctgtagttct attctatggc
     8341 tgttccgaca gtgattctca taaagaatgt gattgtatca atagattgga tgatgtttga
     8401 aaagtagaga atagttgagg ctcttcctgt aaaacaaaaa aataattatg tttgtagagg
     8461 aagtagtcat tccactacaa aaaaattccc ctggatcgtg ggaggtgtga aaaaaatggc
     8521 gagtgttggc gaggatattt tgcaaggcag cggagacatt atgttgtcat ggtgaatata
     8581 aaaagagact ttttaatata tggatgttag cttttgaacg gtggaggaat acatacagta
     8641 cctgtcaacg gtaagcggac ggaatgaagc gatagcgaaa tgatggagca ttaaggagtt
     8701 gatagggaac tttgaccaag accctcttgc agtcctcaaa acgcagtacg ttttgggctg
     8761 gctaccgccg agggaatccc cttggagaag gggttggggg attgatattt ttatatgggg
     8821 ttgggcaaag cttcagttaa ggagtgttgt ttggatgcac ttatagtccc tttagacgta
     8881 aagcgcagcg gaacataatt gtattaagaa tatttgttct ccatgctatt gtgtgactct
     8941 aattcacata tagtcataca aagtaatatt agtttcactt taatgttgta tgaaggtatt
     9001 gcatattaca cattgaattt tatttgtgtg ggatatatgt gaaggggatg aaagcaatat
     9061 gaaggtgttt gaagtgttag aaagcctcaa gaaaggcgag aaattgaaag agatagctaa
     9121 tgttttagga attaacaaca gcacgttaca aagaaagctg aaggctttgg ggtatgcttt
     9181 tgatagtagc actaaagaat ggttctacaa tgacaaagat aacccagagc cattagaaga
     9241 aaatattatg gatatggtga agcgaaatag taaaagtggt gttaatgatt tacctaagat
     9301 aatacaaggt attaaaagcg agactaaagg tgaattagga gtgaaacatg gtaatacagt
     9361 gaaagtatct gaagttttga caagtgaaga tatagcagac cttcatgaaa tgctcttatt
     9421 ttggcgtaca gatagagaaa gaacgaaaaa gcacaaaact cttcatgaag aaataaaaac
     9481 tcttagaagg gacgatagga cgcgaaaaac ggttattttg agcaaagaag ttgcgaaacg
     9541 tctcgataaa ctgtcagaaa gagagcgttt tgacaagtcc gacctattag aattggcatt
     9601 ggagcatttt ttgagtgaat atgaatgaaa atagattgtt gtttgttatt aggtccatta
     9661 tggacctttt tatttttttt agggaagatt gtagagcttt gtataacgtc ctcgcaaatt
     9721 attgttattg atcttacgag gtttgtagag ccttgcataa tgtcctcgca aattgttgat
     9781 gtgagtatat ggggatatta tctaaaactt ttggagaatg gctattggca tcctaaggat
     9841 gccacagaag aattgaatgg attattagca gaaggataga aagtaataaa aaaagaaccc
     9901 tgtaaatagg attctttttt tgtggttatt gacatataaa ttgtgtcttt tagaatgtat
     9961 tcaaagttac ttatagattt ttaagtcgta atagaatgac taagttgttt gacgagaaat
    10021 tttgcatact ttttttcgat ttcggctgtt attataccgt tttctaaaag tatgtaagtg
    10081 ttgttttgtt cgtcagttag taatctgatg ttatcatttt ttggggaaat ggcaacatct
    10141 ttttcttttt cattgatatt ttttaattgt atttgcggta atgggggtac agttttgata
    10201 agtttgttca tttcttcttg aaattctttt gttttaactt tacccatgtt tgttttaagt
    10261 aaattacatt ttcgtagatc tagagggttt cctgtgatgt tgtaacatgc ttctttttgt
    10321 gtgttgcatt ctaggagtaa tcgagatagt tgtgtttttg aactttcacc tttttttctt
    10381 ccgatgatat tataccgcat tcgttttcct tggctattaa ctaagtagtt ttcccagtaa
    10441 aaatcgtgag aattagctat ttcgaagcaa tctgtatcaa taaagaaagc agtttcttta
    10501 ttattggagt ctttgaaaaa aattgccgta tagccatttt ttaagtgttc atatctattt
    10561 ttcatgaatt tcctcctctc gttaaagatt atagaatatt cagaactaca ataaaaggtt
    10621 gtaataaaaa aagacgtggt atttgaccac gtctttt
//
