LOCUS       NZ_CP083095            12887 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Bacillus thuringiensis strain B401 plasmid pP05_2-j, complete
            sequence.
ACCESSION   NZ_CP083095
VERSION     NZ_CP083095.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214334
            Assembly: GCF_020809125.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x, 60x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809125.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,136
            CDSs (total)                      :: 6,981
            Genes (coding)                    :: 6,638
            CDSs (with protein)               :: 6,638
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 343
            CDSs (without protein)            :: 343
            Pseudo Genes (ambiguous residues) :: 0 of 343
            Pseudo Genes (frameshifted)       :: 120 of 343
            Pseudo Genes (incomplete)         :: 233 of 343
            Pseudo Genes (internal stop)      :: 92 of 343
            Pseudo Genes (multiple problems)  :: 78 of 343
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083095.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="B401"
                     /isolation_source="Biopesticide B401/B402 (Certan),
                     Vita-Swarm"
                     /db_xref="taxon:1428"
                     /plasmid="pP05_2-j"
     gene            join(12426..12887,1..351)
                     /locus_tag="K8Z39_RS35145"
                     /old_locus_tag="K8Z39_35010"
     CDS             join(12426..12887,1..351)
                     /locus_tag="K8Z39_RS35145"
                     /old_locus_tag="K8Z39_35010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000734782.1"
                     /translation="MKKLLISFIAILFFICGFNLKAFAAEEVIDYQSLYNQAIQEGVLD
                     QNSVSYNEWLKQNKEEFMPIYQDGLKQGVFLEPLSYNEWLKLNNYGQAPMGDTEFFDDV
                     TPKGSWGGFTLKAGDIFITNATSSAGIVGHAAIANGDNYILHMPGAGQGNQQLSTSNWM
                     QKYTASGKWIKVYRLKDQTLARDVARYADRNFYSTTGSATKNVYLDYGIDTHLYQKNPT
                     YCSKLVFQAFYFGSGSRNVMQAVSGIVTPYGLIDTFTSAYRPSLVKTY"
                     /name="hypothetical protein"
     gene            398..739
                     /locus_tag="K8Z39_RS35150"
                     /old_locus_tag="K8Z39_35015"
     CDS             398..739
                     /locus_tag="K8Z39_RS35150"
                     /old_locus_tag="K8Z39_35015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_000988278.1"
                     /translation="MMTIFISIVLVVSFVLFNRVHAKKSIYNYIARQGIQESQLKYIDF
                     RKDFKIGGYWLAVYVEGENPDIYYEYSYQDKKVNFQAYFNSEKAIKKKMWGGSGLTETE
                     MKKLKYPPL"
                     /name="DUF3139 domain-containing protein"
     gene            1341..2678
                     /gene="mobV"
                     /locus_tag="K8Z39_RS35155"
                     /old_locus_tag="K8Z39_35020"
     CDS             1341..2678
                     /gene="mobV"
                     /locus_tag="K8Z39_RS35155"
                     /old_locus_tag="K8Z39_35020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031674.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_048556187.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDMFTRKELTSLQQDF
                     PMEMREKGFDVERGEGSEKKHLSPQAFKEKQDLQVEVEQLSNVKTHLKTKVVETHNQLQ
                     QTTNYIEKQNETLQKIQQQFLNLDKKIKEKKQEFETFRNQVPDKPVSMSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENQSLRE
                     DNKYLEETIEGNNLALKHSYKQNRELEEVNKELHTEIGTLKAHIRDLQMNIKVLYQQTK
                     KVFKEQFKAFRGLIKNELDMKGVDNQFEREHTREIRSRQKGYDMER"
                     /name="mobV"
     gene            complement(3552..3740)
                     /locus_tag="K8Z39_RS35160"
                     /old_locus_tag="K8Z39_35025"
     CDS             complement(3552..3740)
                     /locus_tag="K8Z39_RS35160"
                     /old_locus_tag="K8Z39_35025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031310435.1"
                     /translation="MVFFRNAGGTPSNKSTSILSSGKPAKARGYVTLQSGFFTGDTIWN
                     ISISNGSSVSSSITKTR"
                     /name="hypothetical protein"
     gene            complement(3775..6738)
                     /locus_tag="K8Z39_RS35165"
                     /old_locus_tag="K8Z39_35030"
     CDS             complement(3775..6738)
                     /locus_tag="K8Z39_RS35165"
                     /old_locus_tag="K8Z39_35030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538378.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_000538375.1"
                     /translation="MGVKQLLSEAQRNELMDLSRLTEWDLVTFHTFSKHDLHLILKHRR
                     GYNRLGFALQLALIRYPGWSLTEYKDIPQYVVAYVASQLQIPPEEFLVYAKRGNTLWEH
                     LGEIRTEYGYQNFSSEYKETLLQFLVQQAMDNNNTLYLIEITISTLRKMKVILPAMYVI
                     EDIVWEAKQQADQKVYSILHDGLVQEQKDQLDALLLPTINGKSPLAWLKDVPAQPSPES
                     FLKVIDRLQFVQKIGLTIDTTKINTNRLRQLARLGSKYEPYAFRRFNEVKRYSMLVSFL
                     LEITQDLIDYAIEIHDRLMMNLQTKGKKEQDEIQQANGKKLNEKILQFITVCGTLIEAK
                     ETGKDAFAALDEVMSWNEMVESVEEAKQLSRPLNYDYLDLLNTRYSYVRRYAPTLLRSL
                     HFRATKSGEPVLQALDTIHELNETGKRKVPHGAPLHFVSNRWQKHVYDDDGNINRHYYE
                     LAALTELRNHIRSGDIFVSGSRHHKAFDDYLIPYDEWNEVSNIPNGLTAPLKAEDYITD
                     RINRLNEHLEWLSKNSEKLEGVDISQGKLHVERLDRGTPEEAKAFSKLLHSMLPRIKLT
                     DLLIEVASWTGFHDQFIHASTNQSPDQEEQNIVLATLMAMGTNIGLTKMAEATPGISYR
                     QMANASQWRMYDDAMVRAQSILVNFQKEQKLSSYWGDGTTSSSDGMRLSIAVRSLHADS
                     NPHYGTGKGGTIYRFVSDQLSAYHVKVITTNARDALHVLDGLLHHETDLKIEEHYTDTA
                     GYTDQVFALTHLLGFRFAPRIRDLADTKLFSIPGGEEYENVQALLKGKINVKLIKENYE
                     DIRRLAYSVQTGKVSSALIMGKLGSYARQNKLATALGEMGRIEKTLFTLDYISNKAVRR
                     RVQKGLNKGEAINALARIIFFGQRGEFRERALQDQLQRASALNIIINAISVWNTVYMEK
                     AVEELKARGEFREDLMPYAWPLGWEHINFLGEYKFEGLHDTGQMNLRPLRIKEPFYS"
                     /name="Tn3 family transposase"
     gene            complement(6757..7611)
                     /gene="tnpI"
                     /locus_tag="K8Z39_RS35170"
                     /old_locus_tag="K8Z39_35035"
     CDS             complement(6757..7611)
                     /gene="tnpI"
                     /locus_tag="K8Z39_RS35170"
                     /old_locus_tag="K8Z39_35035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnP I resolvase"
                     /protein_id="WP_000382147.1"
                     /translation="MDVAKQFSSYLKQENKTENTVQGYTSGIRQYIKWFEGSYDRKLTK
                     LYRQNILEYISYLKNVKMLNAKSINHKISSLAKFNEFLIQKGSQQDQVILKTDMIKVQT
                     VYASPTQIVELDVKKFLQSVLEDNNKRNYAIATLLAYTGVRISEALSIKMNDFNLQTGE
                     CIIRSGKGGKQRIVLLNSKVLSAIKDYLIDRKTYSTAHESPYLFISKKREKLDRTVVNR
                     IFKSYSNVITPHQLRHFFCTNAIEKGFSIHEVANQAGHSNIHTTLLYTNPNQLQLKNKM
                     ELL"
                     /name="tnpI"
     gene            complement(8189..8602)
                     /locus_tag="K8Z39_RS35175"
                     /old_locus_tag="K8Z39_35040"
     CDS             complement(8189..8602)
                     /locus_tag="K8Z39_RS35175"
                     /old_locus_tag="K8Z39_35040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729666.1"
                     /translation="MKKILFFILTIVMISTFSLPNKYVQAAEISKQPSLDTYLTPEIEK
                     EINNNMQPATKENPISSYTASNGLKVVDTVKTEPAFSDSFSSTSYVTHSKTFYASGLSI
                     ATYKGKWLCTFWRDGSVTIKNWDYFLVGIKGYV"
                     /name="hypothetical protein"
     gene            complement(9528..9686)
                     /locus_tag="K8Z39_RS35180"
                     /old_locus_tag="K8Z39_35045"
     CDS             complement(9528..9686)
                     /locus_tag="K8Z39_RS35180"
                     /old_locus_tag="K8Z39_35045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000758643.1"
                     /translation="MKKVYLTILGISLISILSLGLNDSFKNQAVNIGDGGGAPARPDYF
                     LQTHSNR"
                     /name="hypothetical protein"
     gene            complement(9686..10777)
                     /locus_tag="K8Z39_RS35185"
                     /old_locus_tag="K8Z39_35050"
     CDS             complement(9686..10777)
                     /locus_tag="K8Z39_RS35185"
                     /old_locus_tag="K8Z39_35050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102654.1"
                     /translation="MNVQLENEQITKLLNDWYQSMLKQQLVKAKQLKEYIDSEINNVKE
                     NQNLLLYYSLLDFRYKALTDWISINENSFDEMDNFTTPADDFLAYYYHFFKAFHSTLTS
                     NYTEASEHYEKAKELLIHIPDPIEHAEFNYRMGYFYYQIYKQVIALDYIKLAKEEFSKH
                     EGYEINIALCDNFIGLCCIDLKHFELAEESFNKALDTFQKNNHKNSMLMVRHNLAWLYS
                     KQNLSKLAIRHVSEVTKNNPNHFKAFFVEAFEYYKINDHNYAEQLIEKGLKLSNDFNNQ
                     EFQHRFKILKALNNKVPILTLETSISEGITYFKQEKLWECVKEYADILALRFYEENNHN
                     KASQYFYMSNTAQKNELEKGALK"
                     /name="RapH N-terminal domain-containing protein"
     gene            11238..11849
                     /locus_tag="K8Z39_RS35190"
                     /old_locus_tag="K8Z39_35055"
     CDS             11238..11849
                     /locus_tag="K8Z39_RS35190"
                     /old_locus_tag="K8Z39_35055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000185570.1"
                     /translation="MTNTIDFKQVEKNARIRDFENEKEKFKQDHNGINQEEINQAMQVL
                     SKATGGKEVFIGTKRSPQSRVKFAQFIQDNIDQLEEINYLDNKEIVVIWKISRKIGFLS
                     NCIVDDIHKKNPVPLTQTEIALLLKRTKNNINPIIKSLVDKGILAVSEIGIAGNNVRAN
                     AYFMNPNIIFAGNKDDVNPTLKAMFHRVPKELKNLPVKLF"
                     /name="hypothetical protein"
     Typing          1342..2675
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.202"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caacaattat ctacatctaa ttggatgcaa aaatatactg cgtcaggaaa atggattaag
       61 gtttatcgat taaaagatca aacacttgct agggatgtag ctagatatgc tgatagaaat
      121 ttttattcca ctactggaag tgcaactaaa aatgtttatt tagattatgg tatcgataca
      181 catctgtatc aaaaaaatcc aacttattgt tctaagctag tatttcaagc attttatttt
      241 ggttctggat caagaaatgt tatgcaagcg gttagtggaa tcgtaactcc ttatggttta
      301 attgatactt ttacttcagc atatagaccg tcattagtaa aaacatatta ataaataatt
      361 taggaggatt aaatttgaaa aaaaaaagat gttaataatg atgactatct ttatatcaat
      421 agttttagta gttagttttg ttttatttaa tagagtacat gcgaaaaaat ctatatataa
      481 ttacattgct agacaaggaa tacaagaaag tcagttgaaa tatattgatt ttcgtaaaga
      541 ctttaaaata ggtggatatt ggcttgctgt ttatgttgaa ggagaaaatc ctgatattta
      601 ttatgaatat tcttatcaag ataaaaaagt taattttcaa gcttacttta attctgaaaa
      661 agctataaag aaaaaaatgt ggggtggaag tggacttact gaaacagaga tgaaaaaatt
      721 aaagtatcca cctttgtaat aataaacagt ataaaaaaga acttaaataa aggataaaat
      781 aaagactaat tagtattaaa tagataagta ctaattagtc tttttatttt tttttaattt
      841 agtattaatt agaattaaga gtttgataaa ggatttaatg agcgatgggg gtaacttgca
      901 aagtgactat tgggagaaga aaaataagcg gtaagtgtca atggtaagcg gactggaata
      961 gatcagcggt ttgtgctgat ctatgaaagg agcattaagg aatggacacg tgcagccgat
     1021 tttccatcct cttggcagtt cctcaaaacg aaccgttttg gggacgccaa ccggcgagga
     1081 agcccctcaa ggatttgagg ggttggggag ttcgattgat ggggtcaagg ggaagagttc
     1141 cccttgtggg gtgtggggca acgcccctgg tctttatcac gctgtggcgt gatttggcga
     1201 agccaacgag gtctcgcaga gcccacacca aatgtaattt ggtatagtgg gctataccaa
     1261 aactttggat tcgctctctc gatatgttat gatagtctca aaagtagtcc tcttaccgaa
     1321 gacaattgtg agggaaaaac atgaacaaat tcgcaatcat tcacatgcag aaatttcaaa
     1381 tttctgatgt gcaagggatt caaaaacata atcaaagaca aggaaaaagt aaatcaaatt
     1441 tagacatcga ttactcgaaa agtgaacaga attatgattt gttgaatcag caaaaaatta
     1501 gatatgaaag cacaattaaa caagagatta gtgaacgagt aaaacgaaag ccacgagcca
     1561 attcggttgt actttcagaa tttgtagtaa cagcttcgcc tgattacatg cattctttaa
     1621 gtttagaaga acagaaacgt tattttgaga gttcattaga ttttattcaa aaacgttatg
     1681 gcaaacaaaa tacgttgtat gcgatggttc atatggatga agcgacacca catatgcata
     1741 ttggtgtcat gcctattaca gaagacaatc gtttgtccgc aaaagatatg tttacgagaa
     1801 aagagctcac atctttacag caagatttcc cgatggaaat gcgggaaaaa ggctttgatg
     1861 tagaacgggg tgaaggttct gagaaaaaac atttatcgcc tcaagccttt aaggaaaaac
     1921 aagatttaca agtagaagtg gaacagttat caaatgtaaa aacgcattta aaaacaaaag
     1981 tagtggaaac ccataatcaa ttacaacaaa ctacaaacta catcgaaaaa cagaatgaaa
     2041 cgttacaaaa aattcagcaa caatttttaa atttagataa aaaaattaaa gaaaagaaac
     2101 aggaatttga aacgtttcga aatcaagttc cggataaacc agtgtcaatg tcttatttgc
     2161 gagaagaaac aaaaacagaa gtaacaacga aactgtttgg aaagccagaa attactgaga
     2221 agaaaacagg aaacattgtg gttacacgtg agcaatggag agatatgaca gaaaaagtga
     2281 atgcagccgt tattgttaaa aaagattatg aacgtttaca gaagacggat cttgtaaaag
     2341 agaatcaaag tttacgagaa gataataaat atttagaaga aacaatagag gggaataatc
     2401 ttgctttaaa acattcttat aaacaaaatc gggaattaga agaagtaaat aaagaattgc
     2461 atactgaaat aggcacttta aaggctcata taagagattt acagatgaat ataaaggttt
     2521 tatatcaaca gacgaaaaaa gtctttaaag agcaatttaa ggcatttaga ggacttatta
     2581 aaaacgaact ggatatgaag ggtgtagata atcaatttga acgtgaacat acaagagaaa
     2641 taagaagtag acagaaagga tatgacatgg aacgttagaa acgttttgtg atatactata
     2701 attattcatc actaactaat ttatgggttt catccattta aaaatctctt tatatgtgtg
     2761 catataaaga gattttttgc gtttatgtaa ttaaaattat gttaagattg ataagactac
     2821 atttgaaaat gtgggttcaa gttgctgttt gactgaattg cacaaaaaaa ccaattctat
     2881 tacacgagaa ttggtttttt tgcgtataac gaatagagat atatctaggc gtttaaggag
     2941 ttgtatctta tataatttag tctaacatta ataaaaagaa aattcaaaaa tattcactag
     3001 gtttgttgtt tataaggaat tataaaacat gaaattttgc atattttcta attaattccc
     3061 ggaaatagga attaatgatt tataataaat agatgaaata ccctttcagt ttccgatgaa
     3121 ctgtacttta gtctgttgtt agtacgaagt cttttaaaag acttcgtact ttttttattg
     3181 tcgaccaaga ctatgttgta aatgctcata gcattgtatg ttgaatttca attgtaagtt
     3241 ccaaagtaaa cttttgaata ttagtttact ttcaaattat tgattttata gaactttaag
     3301 agatttttag aaaaatagac atttccataa agaaagtata cattcagaac acaagaataa
     3361 aaggatttcc acaccagacc tttgcccctt agtcttcgca gtaaggaaag gcgcctgtgt
     3421 tagcctattc ggactagcca gtctgtttgc gaagtggggg caaacagaag gaaggcatta
     3481 aacgggattt tatactatgg taaaaaacat tatttattga aaaataaaaa gccacattaa
     3541 tgtggctttt gttatcttgt ttttgtaatt gaactagata cagaagaacc attagaaata
     3601 cttatattcc agattgtatc acctgtgaag aatccacttt gaagtgttac atatccacga
     3661 gcttttgccg gtttaccact agatagtata ctagtagatt tattactagg ggtaccgcca
     3721 gcatttcgga aaaaaaccac gctaaggatt ttttctataa aaagagccgt tatattaaga
     3781 ataaaacggc tcttttatac gtaaaggacg taaattcatt tgcccagtgt catgtaatcc
     3841 ttcaaatttg tattctccaa gaaaattgat atgttcccat cctaacggcc acgcatatgg
     3901 cattaaatct tctctaaatt ctcctcttgc ttttaattct tctacggctt tttccatata
     3961 tacagtgttc cacacactta tagcgttaat aattatgttt agtgcactag ctctttgtaa
     4021 ctggtcttgg agagcacgtt ctctaaattc tccacgttgt ccaaaaaata taattctagc
     4081 taatgcattg attgcttctc ctttatttaa acctttttga acccgtctcc ttacggcttt
     4141 attagatatg taatccagcg taaagagggt tttctcgatt cgtcccattt ctccaagtgc
     4201 tgttgcgagt ttattttgtc ttgcatatga tccgagcttc cccatgataa gagcgctaga
     4261 tacttttcct gtttgaaccg agtatgccaa ccttctaata tcttcatagt tttcttttat
     4321 taactttaca ttaatttttc cttttaaaag tgcttgaaca ttttcatatt cttctccacc
     4381 aggtatagaa aaaagcttag tatctgccaa atcacgaata cgaggagcaa atcgaaatcc
     4441 caacaagtgt gttaaagcga aaacttgatc tgtatatcca gctgtatctg tgtaatgttc
     4501 ttcaattttt aaatctgttt catgatgaag aagcccatct aatacatgaa gagcatctcg
     4561 tgcattggta gtaatgactt tcacatgata ggcagatagc tgatcactta caaatcgata
     4621 aatggttcca ccttttcccg ttccatagtg tggattggaa tctgcatgta gagaacgtac
     4681 agcaatggat aaacgcatac catcagaaga agaagttgtc ccatctcccc aataggatga
     4741 taacttttgt tccttttgga aattcacgag aatagattga gcgcgaacca ttgcatcatc
     4801 atacattctc cattgtgaag catttgccat ttgtctatag gagattccag gggtagcttc
     4861 cgccattttt gtaagaccta tatttgtccc cattgccatc aaagtagcta atacaatatt
     4921 ctgttcctct tgatcaggag attgattggt agaggcgtgg ataaattgat catgaaatcc
     4981 agtccaactg gccacttcta ttagtaaatc tgtcagtttt attctgggta acatactatg
     5041 aagaagttta ctaaatgctt ttgcttcttc tggagttcct ctatccaatc gctcaacatg
     5101 tagttttcct tgactaatat ctactccttc taatttctcg ctattttttg ataaccattc
     5161 taaatgttca tttaatcggt ttattcggtc agttatataa tcttcagctt ttaaaggggc
     5221 tgtaaggcca tttggaatgt ttgaaacttc gttccattca tcatacggta taagataatc
     5281 atcaaatgcc ttatgatgtc tactgcctga tacaaaaata tctcctgaac gaatatgatt
     5341 tcttaactcg gttaaagctg ccaattcata atagtgccga ttaatgtttc cgtcatcatc
     5401 atatacatgt ttttgccaac ggttagatac aaagtgtaag ggagcgccat gaggcacttt
     5461 tcgtttacct gtttcattca attcatgaat cgtatcaagt gcttgtaaaa cgggttctcc
     5521 agatttcgtc gctctaaaat gaagactgcg taataaagta ggtgcatatc gtctaacata
     5581 ggaataacga gtatttaaca aatccaaata gtcataattt aaaggccgtg atagttgttt
     5641 tgcttcttca acagattcga ccatctcatt ccatgacatt acttcatcta gggctgcaaa
     5701 agcatcttta cccgtttctt ttgcctcaat tagtgtcccg catacagtaa taaattgtaa
     5761 aatcttctcg tttaattttt tcccatttgc ttgctgtatt tcgtcttgtt cttttttccc
     5821 ttttgtttga agattcatca taagacgatc atgaatttca attgcgtaat ctataaggtc
     5881 ttgtgtaatc tccaataaaa atgagactaa catactatat ctttttactt cattgaatcg
     5941 tctaaaagca tatggctcat attttgatcc taatctagcg agctgccgaa gacgatttgt
     6001 attaattttt gtggtatcaa tagtaagacc aattttttga acaaattgta atcggtctat
     6061 tacttttaaa aatgattctg gtgaaggctg ggccggtaca tcttttagcc atgctaatgg
     6121 agatttacca tttatagttg gtaaaagtaa cgcatctaat tgatcttttt gttcttggac
     6181 caaaccgtca tgtagtatac tatacacttt ctgatctgct tgttgctttg cttcccagac
     6241 gatatcttca atcacataca tagctggaag aatgactttc atttttcgca atgtagatat
     6301 tgttatttca attaaataga gggtattatt gttatccatt gcttgttgca ctaaaaattg
     6361 taataaagtt tctttatatt cagaagaaaa attctgatat ccatattctg ttcgaatttc
     6421 cccaagatgc tcccataaag tattcccacg tttggcatat actaaaaatt cttcaggtgg
     6481 aatctgtaac tgactcgcaa cgtaagccac tacatattgt ggaatgtctt tatattctgt
     6541 taaggaccag ccaggataac gaattaatgc aagctgaaga gcgaacccta aacgattgta
     6601 tcctctacgg tgttttaata ttaaatgtaa gtcatgttta gaaaaagtat gaaatgttac
     6661 caaatcccat tctgttaaac gtgataaatc cattaactca tttcgttgtg cctctgacag
     6721 taattgttta actcccattg tttccccttt actcctttat aagagctcca ttttattttt
     6781 tagctgcagt tggtttggat ttgtgtaaag tagtgtcgta tggatgttag agtgcccagc
     6841 ttgatttgca acttcatgaa tgctaaatcc tttttcaatt gcattcgtac agaagaagtg
     6901 tcgtaattgg tgtggagtaa taacattgct gtatgattta aagatacgat tgacgaccgt
     6961 acggtcgagc ttttctcgct ttttactaat aaaaagatac ggagattcat gtgctgtact
     7021 gtatgttttt cgatcgatga gataatcttt gatagcacta agtaccttac tatttagtaa
     7081 tacaattcgt tgtttacctc cttttccact tcgaataata cattccccag tctgtaaatt
     7141 gaagtcattc attttgatag ataatgcctc tgaaatacgt actcctgtat atgctaggag
     7201 agtggcaatt gcataattac gtttgttatt atcctctaac acactttgta aaaacttttt
     7261 tacatctaat tcaacaattt gggttggaga agcatagaca gtttgaacct ttatcatgtc
     7321 tgtttttaaa attacttgat cttgttgact tcctttctgt attagaaatt cattaaattt
     7381 agcaaggcta ctaatcttgt ggttaatgga cttggcgttc aacattttga cattctttaa
     7441 ataactaatg tactctaaga tattttgtcg gtacaatttt gttaattttc tgtcatagga
     7501 accttcaaac cattttatgt actgtctaat acctgatgtg tatccctgaa cagtgttctc
     7561 agttttattc tcttgtttaa gataagaaga aaactgtttt gcaacatcca tgtgatttcc
     7621 tccgcgtcta taaattgtgt tgcgtctaaa agtatttatt tatcgataaa ttgtgttgcg
     7681 ttaaacccct agatttatat ttattataac acctaataca acacaattaa tattgtgttg
     7741 tattattttt tgtataaaaa aagacactgt atgtgtcttg gaataatgat atattttctg
     7801 attttttaac tctgattttc ttagcgtggt ttttttccga aatgctggcg gtacccctac
     7861 tataggaacc atcgtttatg gtctttgtgt agcaatggaa ccaaatcacg caataagtat
     7921 ggtctttgtg tagcaatgga accaaatcac gcaataagta ttagcggaca taaaataact
     7981 atttttactg agttaatgtc cgctaatgat acttatagga cattaagtat caaatttatc
     8041 tagttgtgct caaatgacat cgacttttta agttaaccta aatgcacaca aaaaccctta
     8101 ttataactca ggttaactca gttaacttaa tggagtcgct ataccatatg atgtccgcta
     8161 atgcttgttg cgttatcctg ttccattgct acacataccc ctttattcct actaagaaat
     8221 aatcccagtt tttaattgtt acgctaccat ctcgccagaa agtacataac cattttcctt
     8281 tataagtagc aatggaaagt ccactagcat agaaagtttt tgaatgagta acatatgatg
     8341 ttgaagaaaa agaatcagag aaagcaggtt ctgttttcac agtatctaca acttttaggc
     8401 cgttagaagc tgtataagat gaaataggat tttcttttgt tgcaggttgc atgttattgt
     8461 taatttcttt ttcaatttca ggagttaaat atgtatctaa gctaggttgt ttggaaattt
     8521 cggcagcttg cacatatttg tttggtaatg aaaatgtact aatcattaca attgttaaga
     8581 tgaaaaataa aatttttttc ataattgtcc tccttgatat tattatttgg tacatttatt
     8641 atgataatac aaaatattta gaaaataaag acagaaatgt aaaaaggagg tttatttgta
     8701 tgtattttat attgatcctt ttttgtacga ttatttcaac gataattggt ttagttgttt
     8761 ataatttaat ttctagtaat aaaaatgttt ttggaatttt atctgtaata ttgacaaatg
     8821 taatattgat gttgtttttt aacatgattt cattgatttg gtgtacttta attgcaattg
     8881 gtatatatgt tatagttctt aaaatacgtt ttaaaagaaa tataactaat taatagattt
     8941 aaaagattat ttaaagggcc ttaattggcc tttttttgta atttgtaaaa aggaaaggga
     9001 actatttata gagttttttt aactaattct gttttttgtt gtttggatat tttgtggaat
     9061 taatataatt ataaatatga aattgaattt atttaatatt ggggtttgtg tagtaacaga
     9121 acagaaacgg caataaccta tattttcgct tcaccatagc taataataga gtgtttttta
     9181 tcaaaaaaat aaatatctta ctgattaaaa actcaatatt tgttaaattt atcaaaaacg
     9241 gttgttattg ttaatttgat tatgataata gttttgataa cgtagtctta acgattcaaa
     9301 gcctaattta acacagtttt tatcattatc aaaaaccctg atttttgata acatgattaa
     9361 gtcttattgc cgttcttgtt ccattactac acagacccca tattttgtta ttaattttaa
     9421 tataatggtg gtttagtgaa ctttggtata ttcagttttt catttataaa aaataaagta
     9481 taagaaaaca ccttcatttt ttgaaggtgt tttcttatac tttattttta tcggttagaa
     9541 tgtgtttgta aaaaataatc aggtcgtgcg ggtgcaccac caccgtctcc tatatttaca
     9601 gcttgatttt taaaagagtc gtttaatcct aacgatagga tactaattaa agatattcca
     9661 agtatagtta gataaacttt tttcattatt ttaacgctcc tttttctagt tcgttttttt
     9721 gagctgtatt gctcatataa aaatattgac ttgctttgtt atggttgttt tcttcataaa
     9781 atcttagagc taagatatca gcatattctt ttacacattc ccatagtttt tcttgtttaa
     9841 agtaagtaat accttctgaa attgatgttt ctaaagttaa tataggaact ttattattga
     9901 gtgcttttaa gattttaaaa cgatgttgga attcttgatt gttaaagtcg ttactaagtt
     9961 ttaagccttt ttcaattagt tgttcagcgt aattatggtc atttatttta tagtattcaa
    10021 atgcttctac gaaaaatgct ttgaaatgat ttggattgtt ttttgttact tcggatacat
    10081 gacgaattgc cagtttagat aaattttgtt ttgaatataa ccaggctaaa ttatgtctta
    10141 ccataagcat agagttttta tggttatttt tttggaatgt gtctagggct ttattaaatg
    10201 attcctcagc tagttcgaaa tgttttaagt caatacaaca taggcctata aagttgtcac
    10261 atagtgctat atttatttca taaccttcat gttttgaaaa ttcttcttta gctaatttta
    10321 tataatcaag agctattact tgtttataga tttgataata aaaatatccc attcgataat
    10381 taaattcagc atgttctatt ggatcaggaa tgtgtattaa tagttctttt gctttttcat
    10441 agtgttcact tgcttcagtg taattggatg ttaaagtaga atggaatgct ttaaagaagt
    10501 gataataata agctaggaag tcatctgctg gagttgtgaa attatccatt tcatcaaaac
    10561 tgttttcgtt aatacttatc caatccgtta atgctttgta tctaaaatcc aataaagaat
    10621 agtataagag taagttttga ttttctttga cattgttaat ttcactgtct atatattctt
    10681 ttaattgttt tgctttaact aattgttgtt ttagcatgga ttgataccag tcatttaata
    10741 attttgttat ttgttcattt tctaactgaa cattcataga atcttcccct tttccaaaat
    10801 tatctatatc ttgtttttac catgttatca tggtagtaca ttagattaac ttttaaataa
    10861 gttgatatat attattttta atattctgtt tattttatct ttgtttttat atggatgaca
    10921 aaaagccctt ttatatcaag ggctttttgc taatgggcgt aaataagata ttagtcttat
    10981 tgacattttt aggatatcaa atttagttaa aactgtatat atttgttttt cggaatataa
    11041 aaagtcagac aagatataac aaaaaaagcc attcatgcta taattgagtt gtctaagcta
    11101 caactatagg agaagggctt ttcttactgt tatacaatta attagagtgt tcagactcga
    11161 attaatttat ctaactacat aataacaaaa taaaagtctc ttcgctagtt gtaaatacta
    11221 ggaacgagga gaaaagcatg accaatacca ttgattttaa acaagttgag aaaaatgcaa
    11281 gaatacgtga ttttgagaat gaaaaagaaa agtttaaaca agatcataac ggaattaatc
    11341 aagaagaaat aaatcaagca atgcaagtat taagtaaagc tactggtgga aaagaagttt
    11401 ttataggtac taagcgttct ccacaatcaa gagtgaaatt cgctcaattc attcaagata
    11461 atatagatca attagaagaa ataaattatc ttgataacaa agaaattgtg gttatttgga
    11521 aaataagtag aaaaataggt tttctatcaa attgtattgt agatgatata cataaaaaaa
    11581 atcctgttcc attaactcaa acagagattg ccttattgtt aaaaagaact aaaaataaca
    11641 ttaatcctat tattaaatca ttagtagata aagggatttt agctgtttca gagattggaa
    11701 ttgcaggtaa taatgttaga gccaatgcgt attttatgaa tccaaatatt atttttgcag
    11761 gtaataaaga tgatgttaat ccaacattga aagcgatgtt tcatagggtt ccaaaagaat
    11821 taaaaaactt acctgtaaaa ttattttaaa gctgaaattt tcagcttttt tttgttattt
    11881 ttgaagttct taaaatcaag aactttagtt cttgatttta agaacttcaa aaaccttctt
    11941 aaacccttga tataattatg tttttatcct caaacaaggg atgtttactc tcactctcta
    12001 tataagggct aaaagttccg caaggaacag gtcgtatttt tgagtaaaaa gcactcaaaa
    12061 atctccacct ttttcactac gtttcatcct tgccaaactt ttttcggctg gtttttagct
    12121 ttcgccaagc cgaacagacc aaaaacgggg ttaagttgct cgacttggac tagcaattgt
    12181 accagccgaa ccgccaattc ttccgttcaa agcatgattc tttttccata tggatcaacg
    12241 ctatgtgggg tttagccccc acacgactag ccagcactca cacccaaaaa caagggggaa
    12301 ttacattatt tagttagtaa atatttgtat gttttttgtc acaagtagtt acattttgaa
    12361 tttgaaaatc gtgttacctt tataaaaggt taccgaaata taataattat tcagggagac
    12421 aatttatgaa aaaattattg attagtttta ttgctatttt attttttatt tgtggcttta
    12481 acttgaaagc gtttgctgct gaggaagtta ttgattatca atctctttat aatcaagcaa
    12541 ttcaagaagg tgttttagat caaaatagtg tatcttataa tgaatggtta aaacaaaata
    12601 aagaagaatt tatgcctata tatcaagatg gcttaaaaca aggtgttttt ctagaaccgt
    12661 tatcttataa tgaatggcta aaattaaata attatggaca agcaccaatg ggagatactg
    12721 aattttttga tgatgtgact ccaaaaggaa gttggggtgg atttacttta aaagctggag
    12781 atatatttat aacaaatgca acaagttcag caggtatagt aggacatgca gctattgcta
    12841 atggtgataa ttatatttta catatgcctg gtgctggcca aggaaat
//
