LOCUS       NZ_CP083065             5251 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 1613163991 plasmid p2-1613163991,
            complete sequence.
ACCESSION   NZ_CP083065
VERSION     NZ_CP083065.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016544
            Assembly: GCF_021137955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 360.6305x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 03:01:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,298
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,096
            CDSs (with protein)               :: 5,096
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 37 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 12 of 82
            Pseudo Genes (multiple problems)  :: 9 of 82
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083065.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="1613163991"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2-1613163991"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.188053 N 6.143417 E"
                     /collection_date="2020"
                     /collected_by="Dianalabs, Rue de la Colline 6, 1205 Geneva,
                     Switzerland"
     gene            302..1657
                     /locus_tag="K9F41_RS26405"
                     /old_locus_tag="K9F41_26370"
     CDS             302..1657
                     /locus_tag="K9F41_RS26405"
                     /old_locus_tag="K9F41_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_101516568.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATDSGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVAN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            1650..2270
                     /locus_tag="K9F41_RS26410"
                     /old_locus_tag="K9F41_26375"
     CDS             1650..2270
                     /locus_tag="K9F41_RS26410"
                     /old_locus_tag="K9F41_26375"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(2423..2584)
                     /locus_tag="K9F41_RS26415"
                     /old_locus_tag="K9F41_26380"
     CDS             complement(2423..2584)
                     /locus_tag="K9F41_RS26415"
                     /old_locus_tag="K9F41_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            3221..3541
                     /locus_tag="K9F41_RS26420"
                     /old_locus_tag="K9F41_26385"
     CDS             3221..3541
                     /locus_tag="K9F41_RS26420"
                     /old_locus_tag="K9F41_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3750..>4181
                     /locus_tag="K9F41_RS26425"
                     /pseudo=""
     CDS             3750..>4181
                     /locus_tag="K9F41_RS26425"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4219..4704
                     /locus_tag="K9F41_RS26430"
                     /old_locus_tag="K9F41_26395"
     CDS             4219..4704
                     /locus_tag="K9F41_RS26430"
                     /old_locus_tag="K9F41_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            4708..4920
                     /locus_tag="K9F41_RS26435"
                     /old_locus_tag="K9F41_26400"
     CDS             4708..4920
                     /locus_tag="K9F41_RS26435"
                     /old_locus_tag="K9F41_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2935..3107
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(2472..2988)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.462"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3532..5036
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggcctgaaa tcagaccgta tgcatctggc tgcatgacca tgcagccggg taaaatttat
       61 ctgtcacgaa cggggcggtg tatcgggtgg tttgttgccg gttttacctg actgccgccc
      121 cgtttgtggc gaacctgtcc ggagcggtgc gggcaacaga tgttatggta aatagcgtgt
      181 aattataaat agcacaacgt tttttaagga agatgttgtt ccaaaattaa aacatatagg
      241 atatctttcc tatgattgat ttttcgcttt tttggcgaaa aatccccgtt aggaggtatg
      301 catgtttgtt tatttgacta ttgaaaattt taggtcggtt aaggaaaaat cgacattaaa
      361 cttgagcgca tccggttcta gtaaccattt aacaactcat atctataaaa atgatggaat
      421 gaatgttgga acgctcatga gtgctgggat ttatggtgct aatgcgtctg gtaaatctaa
      481 cgttctcatg gcttttgaag ctataaaatt tatcgcaaca gatagcggtg atttgaaaga
      541 aggtaaacgg attccctgct atgagccata tgctctttct tcggctacga atgaggctcc
      601 tgtaaagttt gaggctgaat tttattctgt tgaggggaat cggttcagtt atgaagttgc
      661 ttttattaaa aatcggattt tatttgaatc attagactat tatccatcta gggttaaagc
      721 aaatttattt actagagatg agagcgacac ctgggaaaca atcaagtttg gtgggcatta
      781 taaaggaggt attaaaaaaa taccattctt ccctaataat tcatacttag cgaaagcagg
      841 taataatgcc gcatcaccag acataattaa agaagcttat aactttttta gaaagggaat
      901 tagacatata ggtttaaatg aaaagataag aatatcttct tttggggaga gggaggacgt
      961 cgcaaatgaa acggccaagg ttttatgcat ggttgacact ggtgtttccg gtatatctgt
     1021 acgggaggtt gaggcggaaa taccaataaa aatggatgag gaaatccctc aagaaataaa
     1081 ggagataatt gaagaagatt ataaatatcg ttatatgttc tctcatccta ctgatgaagg
     1141 ggggagtgtt tctttcccta tggggagaga gtctgaaggc acccaaaaat tatttgaaat
     1201 catacctcta attagatctg cgtttaatga tagtatggtt gtaattgttg atgagttaga
     1261 taatagcttg cacccgcata ttgctgactt gattgttaag ttatttaatg atcctgatgt
     1321 caataaaaaa ggctcacaac ttgtattctc aacccataat atgcagttaa tggctccaga
     1381 gaaaatgaga agagatcaga tatggttttg tgaaaaaaat aaaggtgcgt cgtctcttta
     1441 tagcttagat gattttgata agaaaaagat aaaaacaaca acaccttacg cagcttggta
     1501 tgatgagggt aggtttggtg gggttcccga cataaactac ttaaaggtag cctcatttat
     1561 atctggtgat atatccttgg taatgccgga tattgacgtc aaagaattgt ctgatggttt
     1621 ttttgaagag tttgacgggg atctttctga tgagtaaaaa acatggaaat aataagaaag
     1681 tcccacaagt aatgtatata tactgtgagg gtgagaaaac cgaaccatta taccttgagg
     1741 cttatatttc agacagatct aaaagaactt taagtgtatt taaaatacct aaaacaagaa
     1801 aaaatacacc tgagcagttg gtggatgagg cgataaagaa aaagaattca tcatcttccg
     1861 cagaaggtga cgtcttttgg gttgtttatg atcaagagca tctgacgcct tcgtctattt
     1921 catgtcatca aaaggcatgg aataaagcta ataggcatgg gataaatata gcaatatcct
     1981 gtgtttgttt tgaattatgg ttattgttgc atttcggctt cacaaccaaa gatttttcgt
     2041 cgtatgataa tttgatgtct agctccccgc taaaaaaatt aataactaac tatgataaag
     2101 gtagtaaact aacttacctt gctataagag agaggattgg tactgcgaaa gataacgcaa
     2161 gaaagctaga tatttacgct aaaactaatt ataaaaacac gcacgaacct tatctatttc
     2221 catgttatac atcattccat gagctactta aggcgattga cgacttttga agtaatccat
     2281 tgaccatggc ttattacagg ctgtaatcta tgaaaaataa aaaccaccgc taccaacggt
     2341 ggtttttgga aggtacatga gctaccaact tatgtaccgg gtaactggct tagagggagc
     2401 gcagtcacca aaaattgtcc tttcagttta gccttaactg gcgcagaact tcaagactca
     2461 cttcctctaa tccgttacca atggctgctg ccagtggcga tgaaccgtgt ctttccgggt
     2521 tggactcaag atgatagtta ccggagaagg cgcagcggtc ggactgaacg gggggttcgt
     2581 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     2641 gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag
     2701 cgcacgaggg agccaccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2761 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
     2821 aacggcagcg gcgcatcctt tttctgcccc gtttgtcatg ccatatgcct tcgctcttct
     2881 ccgtgtgcag gtaacgcccg ccgcagccga acggcaggag cggcagcgac tgagtgagcg
     2941 aggaggcgtc atatcctctg aacttccatt ttctgcctta cgcccgcagc tgcattttcc
     3001 tctgtcacat gaagcacttt cctgattacc gcactcatgc caacataata agccagtata
     3061 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacccgc taaaagcgac
     3121 tgacgcgcct gcggcttgtc caacaccgtg ccgaccggga aacatttccc gcccgtcccg
     3181 gtgttatcag gaggctactc tggcagacaa acgcaacaag atgctcacga tgtgggtgac
     3241 ggaagacgaa caccggcggc tgctcgagcg gtgcgacggc aagcagctgg cggcgtggat
     3301 gcgtcagacc tgcctcgatg agaagccggc gcgtgccggc agactgccgt cgatatcgcc
     3361 ggcgctgctg cgccagcttg ccggtatggg gaacaacctg aaccagatcg cccggaaagt
     3421 gaatgccggt ggtgccggtc atgaccgggt gcagattgtg gccgcactga tggcaatcga
     3481 cgcgggactg gagcggctgc ggcatgcggt gctggaaaaa gggggggatg atgatcgtta
     3541 aatttcaccc ccgggggcgc ggtggtggtg ccggtccggt ggattacctg ctgggaaaag
     3601 accggcagcg cgagggggcg accgtgctgc agggaaaacc ggaggaagtc cgggagctta
     3661 tcgatgcctc gccgtatgcg aaaaagtaca cctccggcgt gctgtcgttc gcggaggctg
     3721 aactgccgcc cgggcagcgg gagcagatca tggccagctt tgagcgggtt ctgatgcccg
     3781 ggctcgataa agaccagtac agcattctgt gggtggagca caccgacaag gggcggctgg
     3841 agctgaattt cctgatcccc aacactgaac tgctgaccgg gaaacgcctg cagccgtact
     3901 acgatcgcgc cgaccggccg cgcatcgatg cctggcagac cgtggtgaac ggcaggctgg
     3961 ggctgcatga tccaaacgcc ccggagaacc gacggttact ggtcacgccg tccgcactgc
     4021 cggaaacgaa gctggaagcc gctcaggcga ttacacgtgg gttgctggcc ttagcctcat
     4081 ccggggagct caaaacgcgc gaggacgtca caggggcgct cacagcggca ggttttgagg
     4141 tggtccgcac cacaaaaaac agtatcagca ttgccgaccc ggacgggggg cgaaacatcc
     4201 gactgaaggg agccatttat gagcagtctt ttagcgctgg cgaaggactt ggagcagcaa
     4261 tcgaaagcgc agcagcagag taccggcgag atgctgaaag ccgcattcag cgagcacgag
     4321 cagtctgtaa aagcggaact gagcgcaagc gcgaagagaa tcagcgacgc catcagcgcc
     4381 cacgagcagg gtatgaccgc ggcgatgcag tcgaaccggc tgagcgtgct gcgtatggtc
     4441 ggccggacgt ggctgaccat caccctggtc tcggtactgc tgatcgccac gagcgggagc
     4501 attctgtggt ggcaggggca gcagatcacc gggaattacc agactatccg ggcgcaggag
     4561 cggacgcagg ccatgctgtc ggagaagaac cacggcgtgc agctgtcgct ctgcggggag
     4621 aagaaactgt cctgcgtcaa agtgaacccg aaagcgggag cttacgggga ggaagggaac
     4681 tggatggtac tggagaggaa atagcccatg acggaactgg aaaaacagtt gctgaccgca
     4741 ttagagcagc tacatcagga ctactcgcaa aggctggacg agtgggagag cgccttcgcg
     4801 gaatggcaga agatgtctgg tcttatgcaa cgggagaacg cggcgctgaa cgagcgcgtc
     4861 acgcgcttga gtcagcaggt ggcgaacttg agcaggcagt tacagcgttt gagccagtag
     4921 tgcagcggca tgaaatggcg gtggccgccg aacgggcata cgagcagaaa cagcatgaaa
     4981 aagagctggc ggaaacccgt tcccggcagc ggagctatga cggtccgacg ctgggctgac
     5041 tgcgcgcatc cagtgacaaa ccccaagaag aagggctcat aaatttcccg ctaaatacgg
     5101 tcgtgatgtt tccatcacag cgtatacaac ggagggatta tgagactgtt taacgtgatt
     5161 gagacggtgc tgaattgcct gtacatggcg ctggagatct gggactggct ggagaagcat
     5221 tttcctgttt ttaaggcgtt catccggatt c
//
