LOCUS       NZ_CP083058             9730 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 1613258639 plasmid p3-1613258639,
            complete sequence.
ACCESSION   NZ_CP083058
VERSION     NZ_CP083058.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016543
            Assembly: GCF_021137935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 266.8688x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 03:05:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,611
            CDSs (total)                      :: 5,484
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 67 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 23 of 154
            Pseudo Genes (multiple problems)  :: 20 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083058.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="1613258639"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3-1613258639"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.188053 N 6.143417 E"
                     /collection_date="2020"
                     /collected_by="Dianalabs, Rue de la Colline 6, 1205 Geneva,
                     Switzerland"
     gene            join(9496..9730,1..296)
                     /locus_tag="K9F40_RS27835"
                     /old_locus_tag="K9F40_27805"
     CDS             join(9496..9730,1..296)
                     /locus_tag="K9F40_RS27835"
                     /old_locus_tag="K9F40_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(324..503)
                     /locus_tag="K9F40_RS27840"
                     /old_locus_tag="K9F40_27810"
     CDS             complement(324..503)
                     /locus_tag="K9F40_RS27840"
                     /old_locus_tag="K9F40_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            572..949
                     /locus_tag="K9F40_RS27845"
                     /old_locus_tag="K9F40_27815"
     CDS             572..949
                     /locus_tag="K9F40_RS27845"
                     /old_locus_tag="K9F40_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(1011..1310)
                     /locus_tag="K9F40_RS27850"
                     /old_locus_tag="K9F40_27820"
     CDS             complement(1011..1310)
                     /locus_tag="K9F40_RS27850"
                     /old_locus_tag="K9F40_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1300..1563)
                     /locus_tag="K9F40_RS27855"
                     /old_locus_tag="K9F40_27825"
     CDS             complement(1300..1563)
                     /locus_tag="K9F40_RS27855"
                     /old_locus_tag="K9F40_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            1940..3025
                     /locus_tag="K9F40_RS27860"
                     /old_locus_tag="K9F40_27830"
     CDS             1940..3025
                     /locus_tag="K9F40_RS27860"
                     /old_locus_tag="K9F40_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            3022..5211
                     /locus_tag="K9F40_RS27865"
                     /old_locus_tag="K9F40_27835"
     CDS             3022..5211
                     /locus_tag="K9F40_RS27865"
                     /old_locus_tag="K9F40_27835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            6797..8758
                     /locus_tag="K9F40_RS27870"
                     /old_locus_tag="K9F40_27840"
     CDS             6797..8758
                     /locus_tag="K9F40_RS27870"
                     /old_locus_tag="K9F40_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8758..9489
                     /gene="mobC"
                     /locus_tag="K9F40_RS27875"
                     /old_locus_tag="K9F40_27845"
     CDS             8758..9489
                     /gene="mobC"
                     /locus_tag="K9F40_RS27875"
                     /old_locus_tag="K9F40_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     Typing          complement(5417..5997)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8759..9486
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 cgcaatggga acgggacaaa cccgcaggaa tgaagctgtg tgattacctc tatcgcaagg
       61 tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag tgctcaccgg
      121 agcagattag cgcgatgatt gaggtggtca gcgagcggca cggggaaagc gcggtgaacg
      181 agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac gtgccggtgc
      241 ctgaactgcc ggaggacaaa cccgcccgcc ggtggtggcc gttcggcaaa aaataaacgg
      301 cagcaccggc catgccggtg ctgttattcc ttcccgagct tatcccgcat cacctgccag
      361 agtgtttccg catcctcgtg cagccgctcg cacatgtcgg ccggggtatc catatccagc
      421 tcgttcagtt tctcgagtaa cagcagcgac tggtccttaa taaatttagc catattgagg
      481 gcagtttttt cctgcttagt catgttttct gtccttccgg gtgtggtgtg cttcctgtcg
      541 ggtatactgt ctcaaaaccg gaggtgagac aatggcgtgg gtgacggtga gacaggcgac
      601 ggaactgacg gggaaagccc ggagttccct gtatagggat atggcgaaag gccgcgtatc
      661 ataccgtact gaagctgatg gtggtcgggt ggtggatacc agcgaactta tccgggtgta
      721 cggtgaatta agacagattg agactcacga gcgggacggg ttgagactgt cggatgagac
      781 tgaaaaaatg gcctccgacg cgttgatcgc ggagataaaa gcactcaggg aggaggtggc
      841 cggactgcgg caggaaatgc aggccatgag actgctggaa cacaaaccag agacagcaga
      901 gacaccggcc ggaacatcgc gccgatggtg gcagtgggga aaacgctgac gcgctgtgct
      961 tgtccaaccg gcgacggcca cgcaatgaat tgcgccccca tcaccggtta tcagcgaacg
     1021 gtatggggat tctggcgctg gccaaccacg gccagaaccc ggactttcct ggtctggtca
     1081 tcaacatcat agaggacatg ctgtcccatc agactgatcc ggcgaacgtc ctcgccatag
     1141 cgtgagtcca cttcatatag gcgcgggaag tcggccagtt tgtccgctgc agcgagaact
     1201 cgatcggtaa cgttctgcgc atcccacagg gatgaatttt ccgcaatgta gtcaaacaat
     1261 gctgtcacat cctcgatggc tccctgcttc cattccacct catacggcat cgcgtagttc
     1321 ctgatagcgg ccatgcagtc tctggcggag ttgttccgtg gtcatgcccg ggcgcgtgtc
     1381 agcgcgggcg gcggtcactt tagcgcgaac ctgatcttca tattctgcct gggccagttc
     1441 ttcgttagcc aggcgctgca acagtgcagc tttgtccggg ccaccttcag gcagtatgcg
     1501 gggcagtctc tcggccagtt cccgcaggaa attggcgttt tcatagtgtg tactgttgct
     1561 cattgtggag cctcccgggc tgatacgtcg atatgaagga gtttagcagg gggcggtctg
     1621 aaagaatatg ctcgtgtcag tcaggctgat gaagacgaaa gctggtaaaa aagggcttac
     1681 gacacccatt ttacgtcaaa ctgcacgaca tgcagttgcg tgcatagcca tgcagcgggt
     1741 taaaatttat caggcgcgtt tccggcggtt ttccgggtgg tttgttgccg gttttatccc
     1801 gtagccgccg gaaatgccct cagaccgtct gagcggtgcg cgtaatgcga cgttatggta
     1861 aatcatgtga ttaatggtag tgataatcat tatcactttt gctatattct tgtttctttt
     1921 attgtaagga aatgtaagta tggcaatcac gattaaatcc gccgttaatg tccgtagcag
     1981 agtattaata tactctttac tactgtttac tggttcagcg tatgcggctg atatagctga
     2041 atcaacattc ctgactcgtg atatcggcaa tggtctgtat gaaatgtctt ttgatggcaa
     2101 aaataatagt ctctgggcag catcatcgtc ttcattcgac agagataagg gccacggaat
     2161 tgtttatcag ttagaaccat ccactctgaa aacaaaatct gccgttaaaa tcgcacgtct
     2221 tgggtttgcc acagcactgg atgaggaaca tgagcgactt tatatcggca atactctgga
     2281 cggctcagtg acggtcattg atactctaac cggaaaagaa ctttcagtaa ttccgttaac
     2341 tgagggacag gatgaggctc atttcagacc gaccagagaa atggtcattg acaaaaaaaa
     2401 tcagcgtctt tatgtctcca gcgccgcaga taaaggggct ctgtgggtta ttgacatggc
     2461 tgtcgggaag aaaattaaaa ccattgatgg actggggcac gctgttactg gaattgcgtt
     2521 aaataaaaat ggggataata tttatctggt taacggggat ggtgagatta ttaacctgga
     2581 tgccagtagc tatgaaataa aaaaacggtt tgttgtagag cctgagaaaa aacacttttt
     2641 ccttaatgta agtctggatg aaaaaaacgg acgtgcattt attaccgacc cggatattcc
     2701 cggggtgctg gttgttgatg taaactcggg aaaaatacta cacagaattg atgttataaa
     2761 ttcactggcg attttattta atcctttacg gaacgaaatc tatatcacgc accgtaatgc
     2821 aaaacaggtc agcattgtgg acagtaattc ttattacgta aaaacaacga taaaaacccg
     2881 cctgatgcca aacagtctgt ctttatcaca tgacggtgaa aatctgtacg ttagtgtaaa
     2941 acaagaaaaa aaagagatga gtactatgaa ggattacata ctcaaaatcg aattatctaa
     3001 aattgctatg gagccagaag aatgaataaa tatttagggc tgagcatagt atatatgatg
     3061 tatacatcat ctgttatggc tgatgattct ggcgcagtta gtaaagataa aaatatttca
     3121 ccagaagaaa cattaattgt tcatgggacc gctgtttctg aaagcacaac agaaggaaca
     3181 gattcataca caacccagtc agccaataca gcatcaaaga tgaatctgtc attccgggat
     3241 acacctcagt caactacagt cattacgcga cgaagaatgg atgatgtagc agcaaaaaat
     3301 attattgatg tggttaaaac cgctcctgga ttatatgtca gtacagcaga aggttccgga
     3361 agaccgaatt ttatgtccag aggatttaat gctaatgtca tgtatgaagg atttagcctg
     3421 ccgtggaatt catacattcc aagttcacaa actagttcag ctatttttga ccgggtggag
     3481 atagtccgcg gggcaacagg tcttgttcag ggtgccggag acccttcagc cgcgataaat
     3541 atgatacaca agcgtccgac ccggcaatat caggccagca ctaacatcgg tgccggacgg
     3601 tggaatgact accggggtgg ggttgatgta tcaggaccac tgaatgacag cgcgacctta
     3661 cgcggaaggg cagttgtgga tggcgaaaac agtgagacct tcagggatag tgagaaacgc
     3721 aggaataacg tcatttacgg aattatggag gcagatctga ctgataatac cctacttacg
     3781 attgggggat atcagcagta tgaatataac aatcattggt ggtatgactt acctatttct
     3841 ggtaccggac accaccttaa tcttcctcgc tcgacgttta caggcaatga ctgggagttt
     3901 gaaagaagta aaagtaaaac tgcatttgca actcttgagc agaagttcag taatagctgg
     3961 aagctgaaat tttcaacgct gcaaaactgg agaaatgctg acctgttagg gacggcaaca
     4021 taccggcata aagatacccg acaaagtatg tatcagcaag tatggggatc actgagggaa
     4081 tataattcca aaagctatga cctgtcgctt aatggggact atgctttatt tggtcgtgtt
     4141 catcaattcg tggctggaac aactcagcaa aaatacaccg ataaagatac aggttataca
     4201 tattccccaa tatatattga gaatgttaat atatttgatc atggttcaca ttcttcgaaa
     4261 aaacccatga agatgtcagg tgatgcatcc tacgacgtca ctgaacaaaa aagcgcctac
     4321 gcagcaactc attttctact tagtgataac tggaaaatat tgctgggtgg ccgtcttgac
     4381 tggtatgaat atacaagtaa aggtacatcc attaatacag gttataaagt taccagaaat
     4441 atcaccaaat atgcaggttc aacatacaat cttgacgatc atcataccat ttatgggagc
     4501 tataccgata tattcaatcc acagtcaagc accgacctca atggtaaagt gatcaagcct
     4561 attgttggta agaattatga gacaggtatt aagggtgagt attttaacgg cttgcttaat
     4621 gcctctctgg cggtatttca ggtagaccag gaaaatcgag ccaggctgct tcccgatcag
     4681 acagggtgtg ttactaatgg caatcctcaa tgctatgagg ctagcggtaa agtacgcaca
     4741 aaagggatag atttcgaact tcagggcatg gttacgccac tctgggaaat tggggtggga
     4801 tatacatatg cagatacgcg atatataaaa gatggagatc gtagtaatga aggcaagcga
     4861 ttcaatactg ccgtgtcgcc acaaaattta tttaagattt cgagcatgta cacctttgac
     4921 ggaaaattaa caaactggcg agcaggagta aacacgtact ggcaaagtaa aatatatgct
     4981 aacataacag acacaaggga tggcaatccg gttattaata aacaaaaggc ttattcaaca
     5041 actgacatta ttattggcta tactccaatg ccacaactgg atttacagct caatataact
     5101 aatgtctttg ataaggttta ttatacatcc ataggtgaat cgattggata tggcacggaa
     5161 acatatggag aaccaagaaa tgttaaaatt agtgcaacct atagattcta accatgcgga
     5221 ttgggtcttc tcctcggatt tgcccctata aatccgtctt ttgtggttcc ggcgtacaac
     5281 cccctgtttg ccacttcagt tgatggttaa tcaaatcatg tagaggatct tcttgagatc
     5341 ctccatggtt cgtcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt
     5401 tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc
     5461 acagcaacca aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac
     5521 cgcgtgttta gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg
     5581 gcgtttttgc gtgtctttcc gggttggact caagtgaaca gttaccggat aaggcgcagc
     5641 agtcgggctg aacggggagt tcttgcttac agcccagctt ggagcgaacg acctacaccg
     5701 agccgagata ccagtgtgtg agctatgaga aagcgccaca cttcccgtaa gggagaaagg
     5761 cggaacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgaccc
     5821 gccggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg
     5881 gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaattctggc
     5941 aggagggcga agcctatgga aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg
     6001 ttagtcccct gcttatccac ggaatctgtg ggtaactttg cgagagttag cagcgcccgc
     6061 cgcagtctca cggccggagc gtagcgactg agtgagggag gaggcgcaat atcccccctc
     6121 ccgcagacgg ccaccggacg cgcggatgtg acatattgca ccgtgttacc gcctttccct
     6181 tccctgccga taaatcattc agataggtga agtctgtcgg ttttctgctg aatattttac
     6241 gttaacctat ctctgttgct tttttctcct tgcggagaaa tgcaggtcat ccccgctggg
     6301 gcgtggctga cttcaggagg aaatcccctg cttacgctgg ctggacgcca ccgacgcacg
     6361 gtctttcccc ctctttctgg ctcagctcgg ctggacgccg gccacgccag aaattcaccc
     6421 ggcccccgtc aggctcacgc ctgaccaccc acaaaaaccc acggtgctga acgcacctga
     6481 cgaagcagca aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct
     6541 gacgggggcc ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc
     6601 tggggcgtgc tcgttgttcc tccggggctg cgccttttcc gcttggggcg gaaagaggct
     6661 ggcctccgtg gtgaaagctg aatgcaatca ccacggacgt gaccgccgcc gccatgcctc
     6721 ggggcatgtc cgcggcttgt gcagcagggg ctgcacacca cgccggtaaa cccgtcgtct
     6781 gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc tggcagtggc atatcccgga
     6841 gctggcccgg cagatttacc cgtgggtcag ctggctgtac agggagtatt acggtctggc
     6901 cggggctttc ctggttatcg gcctgttttt cggtctggct gaactctggg ccggacggat
     6961 gcgttttgtg gggctgagtc ataaggagcg acgggagtat gccgatcgct ataagcgcga
     7021 taataaccgg actgcgccat tttcggtgct ggcttcgctg gattcgcccg ggcggatggt
     7081 cagggcacgg cggcatgtct ggaactacgc gtactggctg accggcgctg ccggtctggc
     7141 ccttctgggg gggctggttg tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg
     7201 tccggacctc tacgcgaacg cagattatta cctgctgcag ccggctaacc tgttcgggat
     7261 tgtctggtgt gccggagggc ttgtcgccgg catccttctg gcgcggctgc tggcatttct
     7321 gctgcttgac ggtcattttg cggccgctga cgaggcggtg aatgcgaaac tgaaccagga
     7381 gagccggcgc agcagccagc ggaccggaga aatgaccgac gtccgccacc tgcatttcgg
     7441 ggagccggta ccggttaacg cgctggccga tttttccaca gagcaggccc ggaaacagca
     7501 ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg ctggtcccgc gtgatacctg
     7561 gcgcaagacc aatattcaga tcctcggcct gcccgggagc ggcaagagcg tgatgggcac
     7621 caacgcgctc attcgctgcg tacgcgattt cggggacgcg gtggtgtact tcgaccccaa
     7681 aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc ccggacttca ccctgctgga
     7741 tctgcggccg gggaaaccgg cgcagctgaa tctgtttcgc gatctcgacc agtacgccct
     7801 gaagaatctg ctggttgccg ggtttaacct gagtgagacc ggcgacgtgg cggaccacta
     7861 ccgcatcagc gagcagaaag cggcgaagct gattgcggag cagttcccgc agggggcaaa
     7921 cattcagcag gtgctggcgg cggcatacgc gctgccggag gccctgaaaa aggacgtgaa
     7981 ggggctgatc accaagctgg agaacgtggc cgacctgagc gtcctgcaga cggacagcgg
     8041 catcgacgtg gcggggataa tcaacggcgg cggctgcctg tacgtcatcg gctcgatgga
     8101 cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc cgctgcgcgc aaatcatcat
     8161 tgcgcgggat gagttccgga ggtggccgca cgcgagcatc atgctcgatg agattaaata
     8221 cctgctgtcg aagtacgtgc tgaacgcgct ggggacgctg cgcagtcggg actgcaacct
     8281 gctgctcgcg caccagtcgc tgggcgactt cgggcagtgc ggccaggatc tgcccgctga
     8341 gtttgtgaaa accacggtgc tggataacac gccgatccgc tggttctacc gggcggccag
     8401 ccaggagtcc gcgcagtggg cggcgggcca gaccggggaa atccgggtgg acgtggagcg
     8461 ccgccgggcc agccgggagg cggggaacgt ggagcatatc agcggggaca gcttcattca
     8521 gaaggaggcg cggccgctgt ttgacgtgaa caccctgcag cacctgcctg acgggtttgc
     8581 ggtgatgacc gggctgggcg tggcccggat ggcgttcagc agcccgctgc gggttgaacg
     8641 ccgggaaatc ccgctgcagc gcttcccggt gctggcgaaa acggacccgc tggcggagta
     8701 ccagccggag gccgggcggc agcggccggg tgatgatgat tttgcggggc tgtactgatg
     8761 gcactggagc gatacaacgt cagccacgcg aagcggcagg cgcggaacgc ggagaaaacc
     8821 cggctgaccc tgcgctggct gcgggaagag ctgtgttcca cggcggagct ggttgcgcga
     8881 cggctgggga ttgcggcggt gcagccggtt taccgcttcc tggacagcct ggtggcgaaa
     8941 gggctgctgg tccgggcgaa atacccggtt gacggccgtc aggtcagcgt gtggggactg
     9001 acgccgcacg gggtggcgtt ctcgttcgac gaggacgaac cgctgacgga cgttattccg
     9061 tttcagccgt caagggtgtc ggcggcgcag ctgccgcacc ggctggccgt gcagtccctg
     9121 cgtctcgcca tggaggcccg gggggcctcc ggctggcggt acctgcaccg tatggccctg
     9181 aaggggatga aggtgccgga cgcgctggcg gagctggacg gcagaacggt ggcgtttgag
     9241 gtggagcgca ccgtgaagag ccgcaggcgc tatcaggagg tggtgtccgg gtatctgttc
     9301 aatcgcaaag cgaacggcat cgatgaaatc tggtacatct gcccggaccg ggcgacgcag
     9361 gtcagggtgc agcgggcgat cctgtcggtg gatgaaatcg tgaatccaca gaccggcgag
     9421 gcccgcaaaa cggcggagct ggaccgggag cggctgtttg cgtgctttaa atttatgaca
     9481 acggagtgag ggattatggc gctgtacatg gtatttattg aagacgcgga ggaccgggac
     9541 ggcgagcgct gcatcatgcc gaccagcgag catcttgaag atatggtggt gctcgcctgg
     9601 cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa
     9661 tccatcggga tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcacc
     9721 ggtaaaaatc
//
