LOCUS       NZ_CP083018             5359 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 6709.15-I plasmid p2-6709.15-I,
            complete sequence.
ACCESSION   NZ_CP083018
VERSION     NZ_CP083018.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016534
            Assembly: GCF_021137775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 328.832x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 03:02:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,453
            CDSs (total)                      :: 5,332
            Genes (coding)                    :: 5,190
            CDSs (with protein)               :: 5,190
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 52 of 142
            Pseudo Genes (incomplete)         :: 81 of 142
            Pseudo Genes (internal stop)      :: 30 of 142
            Pseudo Genes (multiple problems)  :: 16 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="6709.15-I"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2-6709.15-I"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern, Friedbuehlstrasse 51, 3001 Bern,
                     Switzerland"
     gene            complement(join(5295..5359,1..256))
                     /locus_tag="K9F34_RS27115"
                     /old_locus_tag="K9F34_27075"
     CDS             complement(join(5295..5359,1..256))
                     /locus_tag="K9F34_RS27115"
                     /old_locus_tag="K9F34_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_042863636.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1247..1921)
                     /locus_tag="K9F34_RS27120"
                     /old_locus_tag="K9F34_27080"
     CDS             complement(1247..1921)
                     /locus_tag="K9F34_RS27120"
                     /old_locus_tag="K9F34_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_032419561.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRARESQL
                     IQVKFFTAEIKAKFASRGELAHQAQQAYHRALVQIYGDKLQIIKGYYSERQECAMTFNE
                     GHPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEVEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISNLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            2269..2685
                     /locus_tag="K9F34_RS27125"
                     /old_locus_tag="K9F34_27085"
     CDS             2269..2685
                     /locus_tag="K9F34_RS27125"
                     /old_locus_tag="K9F34_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032419562.1"
                     /translation="MSDQKNNLDIQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            3103..3366
                     /locus_tag="K9F34_RS27130"
                     /old_locus_tag="K9F34_27090"
     CDS             3103..3366
                     /locus_tag="K9F34_RS27130"
                     /old_locus_tag="K9F34_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054175658.1"
                     /translation="MAAYTSETEKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="hypothetical protein"
     gene            complement(3916..4128)
                     /locus_tag="K9F34_RS27135"
                     /old_locus_tag="K9F34_27095"
     CDS             complement(3916..4128)
                     /locus_tag="K9F34_RS27135"
                     /old_locus_tag="K9F34_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4132..4617)
                     /locus_tag="K9F34_RS27140"
                     /old_locus_tag="K9F34_27100"
     CDS             complement(4132..4617)
                     /locus_tag="K9F34_RS27140"
                     /old_locus_tag="K9F34_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<4655..5332)
                     /locus_tag="K9F34_RS27320"
                     /old_locus_tag="K9F34_27105"
                     /pseudo=""
     CDS             complement(<4655..5332)
                     /locus_tag="K9F34_RS27320"
                     /old_locus_tag="K9F34_27105"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(365..545)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.978"
                     /coverage="83.0"
                     /evalue="2.08e-53"
     Typing          495..1005
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.969"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(3..256)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.86"
                     /coverage="80.0"
                     /evalue="6.28e-46"
     Typing          complement(3801..5305)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.622"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgccatcag ggcggccaca atctgcaccc ggtcatgacc ggcaccaccg gcattcactt
       61 tccgggcgat ctggttcagg ttgttcccca taccggcaag ctggcgcagc agcgccggcg
      121 atatcgacgg cagtctgccg gcacgcgccg gcttctcatc gaggcaggtc tgacgcatcc
      181 acgccgccag ctgcctgccg tcgcaccgct cgagcagccg ccggtgttcg tcttccgtca
      241 cccacatcgt gagcatcttg ttgcgtttgt ctgccagagt agcctcctga gaacaccggg
      301 acgggcggga aatgtttccc ggtcggcacg gtgttggaca agccgcaggc gcgtcagtgg
      361 cttttagcgg gtttcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata
      421 ctggctcaat atgttacgga ggagtaacac tttatgaaag tgcttcatgt gacaggggga
      481 aaatgcagct acagggcgta caggagaaaa tggaggttac ggtgatatga cgcctcctcg
      541 ctcactcggt cgcttccgct cctgccgttc ggctgcggcg ggcgttacgc acacacggat
      601 aagagcaaag gcatatgaca tgacaagcag ggcgggaaaa ggatgcggcg ctgccgtttt
      661 tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc
      721 gaaacccgac aggactataa agataccagg cgtttccccc tgatggctcc ctcgtgcgct
      781 ctcctgttcc tgccattcgg tttaccggtg tcattccgct gttatggccg cgtttttctc
      841 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
      901 gaaccccccg ttcagtccga ccgctgcgcc ttttccggta actatcgtct tgagtccaat
      961 ccggaaagac acgcaaaaac gccactggca gcagccactg gtaacaggat tcacggagca
     1021 gagtagtaga gtgcttctac agagttcttg aagtggtggc ctgactgcgg ctacactgga
     1081 aggacagttt tggtatctgc gctccgtgaa agccagttac cttcgggaaa aagagttggt
     1141 agctcttgat tccggcaaac gaaccaccgt tggtagcggt ggtttttttg tttgcagggc
     1201 atgagattac gacgagagta aaaggatcac aagaagatcc ggctgtttac cagtgatttg
     1261 gcttcagtat tggcttccgc ctagtgggga ctttatctgg caataaagct aatcttaagg
     1321 cttcatagga tacatatggc aagctccaat cccctaaact agccagatta ctaatcactc
     1381 gactagtatc ctgttccctt ggcactacga ttcctatttt taactcaggc cgatcagctt
     1441 ttatcatttc tattggcggt atcatatctg agtcgttagt gcagagaacc aattgttcta
     1501 cttcaccacg aatcgcatcg cgatacattt ctaaagccat tctaacgtct gtctgctttt
     1561 cttctaactt ccagaccgtt gttctatccg tttttgatgg aggatgcccc tcattaaagg
     1621 tcattgcaca ttcctgacgc tctgagtaat aaccttttat tatctgtagc ttatcgccat
     1681 agatctgtac taatgctcga tggtaggctt gctgagcttg gtgcgccaat tcaccccgag
     1741 aagcaaactt tgccttgatc tcagcagtaa agaacttcac ctgaatcaac tgtgattctc
     1801 gtgctcttat cggaagcagt tgttcttcaa agagcttata aaggtctaac cacttatatc
     1861 ccgaacgctt aaccaatccg tgataaaaat tgaacccatc tacgtaaact attgttctca
     1921 ttttatttcg cgcataaaaa aaccgcctct aagggcggtt ttaacaacaa aaactaacgt
     1981 tagcaaacag tttaagttgt agtacctcag ggttaaaact aaccgagagg gcggtttcaa
     2041 gctcctgaag gaagacgcgt aacttaatcg ctgcccacct aaagggaggt ttagagcctc
     2101 aagggagctg cgtaacttaa tcgcgctacc aagaggtgag tgtagattaa gtatagttaa
     2161 atataatgat attataagcg cactggtgtc gtactaatta tatgaattaa tttaccaacc
     2221 tgctaagctg aagtcttcta agaagacttc agcttagcag gttggtatat gtcagatcaa
     2281 aaaaataatt tagacattca aaaacagaca cttgagtcta aatgggggaa ggaagcgtta
     2341 agcatgggat ggtcagccat tcctacatcc cttttcttcc tgcagggcgc actcaatatg
     2401 acccccacaa cattcaatgt gctacttcat ctaattatcc actggtggaa accctatgat
     2461 tggccacatc catctcaaga gagcatcgcg gttagaatgg gagtttcatc aagaacagtt
     2521 caacgctcac tccaagaact tgaaaattac ggattgctta caaaaattcg tacaagcaag
     2581 gacaacccta agtacaaagg gcgaaacatt tacgatatgt ctcttttagt taagtcacta
     2641 aacattctta cgccagacct caacgccaaa ctaaacgaaa agtaaccaaa aattccgaca
     2701 gccaaagcgc gcacatcact gtacaaaagg tgttcagcat cacatttcta gaaaatgaca
     2761 acagctccct attgaaagct aatttctaaa ctaaacatca gtatcgctac acaaacagca
     2821 atacggacaa aacacaaaaa gaggaattag catgtctaaa gcaccaattg gtacgactgc
     2881 ttctaccgga caaaagtgtc ctgaaagtgg tgtctggaaa gtcgtaggaa ccccttccac
     2941 tacagccccg atagcaaaag gtaatgtaat gcctccgtac gcaggaaagg ctgtaacctg
     3001 gatgttaacc caatatgctt gatatttaga ctggcaacga aaagccgccc cagcgggcgg
     3061 cttttctatc tctgccctat aaattcagaa aaggaaagct aaatggctgc atataccagc
     3121 gaaacagaga agtttttaca aagcgtcagt agcgatcaaa catggtggaa gtcacgatat
     3181 aatccaggag acagcgttcc ccattctgga atttatcgat gtaccgtctg cggaaaagag
     3241 atcaccagta acggcagcga cccgttcccg ccgcaaaacc atcatcagca cagtcagcaa
     3301 caagcaatca aatggcaatt gattgtcaga acggacacta aaggtgaccg tttcggaatc
     3361 aaatgattga gagccctccg gggctttttt tgtacctgaa gctcgaagat atttaccata
     3421 acatcccttg cccgcaccgc tccggacagg ttcgccacaa acgggacggc agtcaggtaa
     3481 aaccggcaac aaaccacccg atacaccgcc ccgttcgtga cagataaatt ttccccggct
     3541 gcatggtcat gcagccagat gcatacagtc tgatttcagg ccagaatccg gatgaacgcc
     3601 ttaaaaacag gaaaatgctt ctccagccag tcccacagct cggtcgccag atacaggcag
     3661 ttaagtacag cttcaatgac gttaagcaat ttcataattc cccctaggta taggccgtgc
     3721 tctttttgat cacgaaccta tttagcggga gaaataagag cccttcttcg tgcggcctgt
     3781 caccggatga tactgttcag cccagtgtcg gaccgtcata actccgctga cgggaacggg
     3841 cttccgccag ctctttttcg tgctgtcgct gctcgtatgc ccgttcggcg gccaccgcca
     3901 tttcatgccg ctgcactact ggctcaaacg ctgtaactgc ctgctcaagt tcgccacctg
     3961 ctgactcaag cgcgtgacgc gctcgttcag cgccgcgttc tcccgttgca taagaccaga
     4021 catcttctgc cattccgcga aggcgctctc ccactcgtcc agcctttgcg agtagtcctg
     4081 atgtagctgc tctaatgcgg tcagcaactg tttttccagt tccgtcatgg gctatttcct
     4141 ctccagtacc atccagttcc cttcctcccc gtaagctccc gctttcgggt tcactttgac
     4201 gcaggacagt ttcttctccc cgcagagcga cagctgaacg ccgtggtttt tctccgacaa
     4261 catggcctgc gtccgctcct gcgcccggat agtctggtaa ttcccggtga tctgctgccc
     4321 ctgccaccac agaatgctcc cgctcatggc gatcagcagt accgagacca gggtgatggt
     4381 cagccacgtc cggccgacca tacgcagcac gctcaggcgg ttcgactgca tcgccgcggt
     4441 cataccctgc tcgtgggcgc tgatggcgtc gctgattctc ttcgcgcttg cgctcagttc
     4501 cgcttttaca gatttctcgt gctcgctgaa tgcggctttc agcatctcgc cggtactctg
     4561 ctgctgcgct ttcgattgct gctccaagtc cttcgccagc gctaaaagac tgctcataaa
     4621 tggctccctt cagtcggatg tttcgccccc cgtccgggtc ggcaatgctg atactgtttt
     4681 ttgtggtgcg gaccacctca aaacctgccg ctgtgagcgc ccctgtgacg tcctcgcgcg
     4741 ttttgagctc cccggatgag gctaaggcca gcaacccccg tgtaatcgcc tgagcggctt
     4801 ccagcttcgt ttccggcagt gcggacggcg tgaccagtaa ccgccggttc tccggggcgt
     4861 ttggatcatg cagccccagt ctgccgttca ccacggtctg ccaggcatcg atgcgcggcc
     4921 ggtcggcgcg atcgtagtac ggctgcaggc gtttcccggt cagcagttca gtgttgggga
     4981 tcaggaaatt cagctccagc ctccccttgt cggtatgctc cacccacaga atgctgtact
     5041 ggtctttatc gagcccgggc atcagaaccc gctcaaagct ggccatgatc tgctcccgct
     5101 gcccgggcgg cagttcagcc tccgcgaacg acagcacgcc ggaggtgtac tttttcgcat
     5161 acggcgaggc atcgataagc tcccggactt cctccggttt tccctgcagc acggtcgccc
     5221 cctcgcgctg ccggtctttt cccagcaggt aatccaccgg accggcacca ccaccgcgcc
     5281 cccgggggtg aaatttaacg atcatcatcc cccccttttt ccagcaccgc atgccgcagc
     5341 cgctccagtc ccgcgtcga
//
