LOCUS       NZ_CP083015             3541 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 6710.71 plasmid p4-6710.71, complete
            sequence.
ACCESSION   NZ_CP083015
VERSION     NZ_CP083015.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016527
            Assembly: GCF_021137755.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205.4498x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 02:58:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,483
            Genes (coding)                    :: 5,339
            CDSs (with protein)               :: 5,339
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 61 of 144
            Pseudo Genes (incomplete)         :: 78 of 144
            Pseudo Genes (internal stop)      :: 26 of 144
            Pseudo Genes (multiple problems)  :: 16 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="6710.71"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4-6710.71"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern, Friedbuehlstrasse 51, 3001 Bern,
                     Switzerland"
     gene            complement(186..587)
                     /locus_tag="K9F28_RS27920"
                     /old_locus_tag="K9F28_27870"
     CDS             complement(186..587)
                     /locus_tag="K9F28_RS27920"
                     /old_locus_tag="K9F28_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(587..778)
                     /locus_tag="K9F28_RS27925"
                     /old_locus_tag="K9F28_27875"
     CDS             complement(587..778)
                     /locus_tag="K9F28_RS27925"
                     /old_locus_tag="K9F28_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1192..1305
                     /locus_tag="K9F28_RS27930"
     CDS             1192..1305
                     /locus_tag="K9F28_RS27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072032591.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPKRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1603..2496
                     /locus_tag="K9F28_RS27935"
                     /old_locus_tag="K9F28_27880"
     CDS             1603..2496
                     /locus_tag="K9F28_RS27935"
                     /old_locus_tag="K9F28_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="WP_032419557.1"
                     /translation="MELNLCWWNIGISPPVKGVKKDKAEAIRLAKHYIKKMSQEKTLDF
                     LGICEISEDEAIDFKPLADKLHLEYLNLSGTVGRIIMDVSIMYEASKLEYIDYKYIIKI
                     QPDDRTLRIAVRVVFKDAVTGNLITFFLSHWPSIRSADEKIREDAAESLRNNVNKILDD
                     HGIESQIICMGDYNTNPYSVAINEKLYATRDYHRIKNKRKLLFNPFWYLLSDKKTNNLG
                     TYHYKSGASNRWFVFDQMLFSSSFIYGGKDCLKLDMNSLDFHRILSDDNQCLDQRFFNT
                     FDHYPIFCRIYHDNQK"
                     /name="endonuclease"
     gene            2480..2917
                     /locus_tag="K9F28_RS27940"
                     /old_locus_tag="K9F28_27885"
     CDS             2480..2917
                     /locus_tag="K9F28_RS27940"
                     /old_locus_tag="K9F28_27885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032419556.1"
                     /translation="MTTKNEEFQERLKASLKKANETLILKEKIESDISSILSMLKGITS
                     DSIDFDIFQNSSKIPKYKDCKKIVRIKKNSSMAYPKGFILFGFSINESTGYPVQVETED
                     EAAECTDVDNLKESIVYIIEKRSIEIMKLISEQQEDDDIPF"
                     /name="hypothetical protein"
     Typing          900..1305
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.643"
                     /coverage="99.0"
                     /evalue="4.5799999999999997e-172"
ORIGIN
        1 aaaatttaaa aaacgaaatt tcaggaaagt aaacaactga tattaaatac aaatctgcaa
       61 aattttaatt ttgcgtagtg tgtgggtatt tttagtcgtg aggagcgttc agagatctgg
      121 cgatttttgg gaaggttcag gaactccccg ggatggccgg agaggaaaac tcaagcatgg
      181 caggatcagg attttttcca gaatttccac catggtgccg gggattccgt taattttgcc
      241 tggtgatttt caagaaggag catggcctgt ttcaggctgt caatgtgcga atccttggac
      301 aaaagtaagg tattcaggtg ttcaatctcc tgtttcagat gacgaatcac ctgttcactc
      361 tggtcagcag aggctgtatc acgctgttca gagacctgtc cagaatggtg ttcacccttc
      421 ggttgttcaa agggaccaaa gacacgtaaa agctctgaag tatctaccat ttttgcattt
      481 cgttcaccgc tacaggtgga caggtcacct gctttaatta acctgtgcag tgttgttcta
      541 ctcttacccg tcagtcgggc agcctcacta atagacactc tggccatcag ttaccatcct
      601 gtaatgcatc cagtctcgag gacagtgtac ggaaaagatg ctccgcatca tcgtggagtt
      661 tctcacaaag gtcggcctcg acatcaagat caagctcgtt aagcttctcc agcaggagca
      721 gagactgatt ctggatgaat ttagccatgt tcagcgctgt tttctgttgt ttcgtcataa
      781 gaagcctcag agcgtcagtg ctcagtgtaa gaacagtctc ttttccgctt cctcgctcac
      841 tcagtcgcta cgctccggtc gttcggctgc ggcgagcggc aggagcatgc gtataaaagc
      901 tcgagaagac cgagcggtca aatggggaag aaggaaaggc cgcaaagcgg ccgtttttcc
      961 ataggctccg cccccctgac aagcatcacc gaagcggacg ctcaaatcag tggtggcgaa
     1021 acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc gtgcgctctc
     1081 ctgttcctgc cattcggttt accggtgtca ttccgctgtt atggccgcgt ttttctcatt
     1141 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     1201 ccccccgttc agtccgactg ctgcgcctta tccggtaact atcatcttga gtccaacccg
     1261 aaaagacacg acaaagcgcc actggcagca gccactggta actgatttag aggagttagt
     1321 cttgaagtta tgggtcagtt aaggctacac tgaaagaaca agttttggtg actgcgctcc
     1381 tctactagcc agttacctag gttcaaaaga cctgcaagtt atttgaaccc ttgaaaaaac
     1441 cgccctgcaa ggcggttttt tcatttccag ccaagaagat aacgccttaa aaggggtagc
     1501 gcaacaccat ttatcaacaa acagataaat aaaatgttta acccccacat caaaagcaat
     1561 acaataaacc caatgaatac aatgcattaa gaaagaaaca ccatggaatt gaatttatgt
     1621 tggtggaata ttggaatatc accacctgta aaaggcgtga agaaagacaa ggctgaggct
     1681 attagattgg ccaagcacta tataaaaaaa atgtctcaag aaaaaacatt ggattttttg
     1741 ggtatctgtg aaatatcaga agatgaggct atagatttta aacctttggc tgataaacta
     1801 catcttgaat acttgaattt atcagggact gttggtcgga taattatgga tgtatccata
     1861 atgtacgaag catcaaaact tgagtacata gattataaat atataattaa aatacaacca
     1921 gatgatagaa ctttgcgtat tgctgtcaga gttgttttta aggatgcagt aacaggaaat
     1981 ctaataacat tctttttatc acactggcca tccataagaa gcgcagatga aaagattcga
     2041 gaggacgctg ccgagtcact gcgcaacaat gtgaataaaa tactagatga ccacggcatt
     2101 gagtcccaaa taatatgcat gggtgattac aacacaaacc cttactctgt tgcaataaac
     2161 gaaaaacttt acgccactag agattaccat cgaataaaga ataaacgaaa acttcttttt
     2221 aacccttttt ggtatttact ttcagacaaa aagaccaata acttaggaac ttatcattat
     2281 aaatcaggag catcgaatag atggtttgtt tttgatcaaa tgttgttttc atcatcattt
     2341 atatacggag gaaaagattg tcttaagcta gacatgaaca gccttgattt tcatcgtata
     2401 cttagtgatg ataaccaatg tttagatcaa agatttttta acacttttga tcattaccca
     2461 atattttgta ggatttacca tgacaaccaa aaatgaagag ttccaagaaa gactaaaggc
     2521 atcactaaaa aaagccaatg aaacactaat tttaaaagaa aaaatagagt cagatatttc
     2581 atcaattttg tcaatgctaa agggtataac atccgactca attgactttg atatttttca
     2641 gaatagttcg aaaattccca aatataaaga ttgtaaaaaa attgtcagaa taaagaaaaa
     2701 ctctagcatg gcatacccta aaggtttcat tttatttgga tttagcataa atgagtctac
     2761 cggatatcct gtacaggtag aaacggaaga cgaagccgca gagtgtacag atgtggataa
     2821 cttaaaagaa tcaattgttt acataataga aaaaagatct atagagataa tgaaattaat
     2881 atccgagcaa caagaagacg atgacattcc tttttaaaaa ggtagcatgt aatgccatta
     2941 tcaacggcac tttttcatag aaggtgccat atatcttaga atcaactaat ttaacataag
     3001 accataattg tacgcaccgc taaaacggcc tcagggcgtt tctgacggga aaccggtaaa
     3061 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     3121 catgagtatg cacgcaagtg catagcgcga aaattcacgc cattcagcca gggtaagtcc
     3181 tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     3241 gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc acgttcagga atcgggttcg
     3301 tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg
     3361 cgactcttca ctagagtcgt acccagagtc gccgcgtcta aaaaacaacc aaacggggag
     3421 tcgtgtccag agtcgggtcg ttattaactc attgttttta tagtcctgag tcgttaccga
     3481 ctctatgacg actcgccggg gactctatga cgagtcgtca tagagtcgga tctatgtaaa
     3541 t
//
