LOCUS       NZ_CP083006             5359 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 6712.08 plasmid p6-6712.08, complete
            sequence.
ACCESSION   NZ_CP083006
VERSION     NZ_CP083006.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016529
            Assembly: GCF_021137715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.2725x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 03:13:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,607
            CDSs (total)                      :: 5,487
            Genes (coding)                    :: 5,358
            CDSs (with protein)               :: 5,358
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 56 of 129
            Pseudo Genes (incomplete)         :: 64 of 129
            Pseudo Genes (internal stop)      :: 28 of 129
            Pseudo Genes (multiple problems)  :: 15 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="6712.08"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6-6712.08"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern, Friedbuehlstrasse 51, 3001 Bern,
                     Switzerland"
     gene            complement(join(4930..5359,1..56))
                     /locus_tag="K9F30_RS27925"
                     /old_locus_tag="K9F30_27885"
     CDS             complement(join(4930..5359,1..56))
                     /locus_tag="K9F30_RS27925"
                     /old_locus_tag="K9F30_27885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064147840.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYRTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEDGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(734..1054)
                     /locus_tag="K9F30_RS27930"
                     /old_locus_tag="K9F30_27895"
     CDS             complement(734..1054)
                     /locus_tag="K9F30_RS27930"
                     /old_locus_tag="K9F30_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_042863636.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2045..2719)
                     /locus_tag="K9F30_RS27935"
                     /old_locus_tag="K9F30_27900"
     CDS             complement(2045..2719)
                     /locus_tag="K9F30_RS27935"
                     /old_locus_tag="K9F30_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_032419561.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRARESQL
                     IQVKFFTAEIKAKFASRGELAHQAQQAYHRALVQIYGDKLQIIKGYYSERQECAMTFNE
                     GHPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEVEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISNLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            3067..3483
                     /locus_tag="K9F30_RS27940"
                     /old_locus_tag="K9F30_27905"
     CDS             3067..3483
                     /locus_tag="K9F30_RS27940"
                     /old_locus_tag="K9F30_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032419562.1"
                     /translation="MSDQKNNLDIQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            3901..4164
                     /locus_tag="K9F30_RS27945"
                     /old_locus_tag="K9F30_27910"
     CDS             3901..4164
                     /locus_tag="K9F30_RS27945"
                     /old_locus_tag="K9F30_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054175658.1"
                     /translation="MAAYTSETEKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="hypothetical protein"
     gene            complement(4714..4926)
                     /locus_tag="K9F30_RS27950"
                     /old_locus_tag="K9F30_27915"
     CDS             complement(4714..4926)
                     /locus_tag="K9F30_RS27950"
                     /old_locus_tag="K9F30_27915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1163..1343)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.978"
                     /coverage="83.0"
                     /evalue="2.08e-53"
     Typing          1293..1803
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.359"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(2..771)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.272"
                     /coverage="92.0"
                     /evalue="1.7999999999999998e-147"
ORIGIN
        1 tgctgcgctt tcgattgctg ctccaagtcc ttcgccagcg ctaaaagact gctcataaat
       61 ggctcccttc agtcggatgt ttcgcccccc gtccgggtcg gcaatgctga tactgttttt
      121 tgtggtgcgg accacctcaa aacctgccgc tgtgagcgcc cctgtgacgt cctcgcgcgt
      181 tttgagctcc ccggatgagg ctaaggccag caacccccgt gtaatcgcct gagcggcttc
      241 cagcttcgtt tccggcagtg cggacggcgt gaccagtaac cgccggttct ccggggcgtt
      301 tggatcatgc agccccagtc tgccgttcac cacggtctgc caggcatcga tgcgcggccg
      361 gtcggcgcga tcgtagtacg gctgcaggcg tttcccggtc agcagttcag tgttggggat
      421 caggaaattc agctccagcc tccccttgtc ggtatgctcc acccacagaa tgctgtactg
      481 gtctttatcg agcccgggca tcagaacccg ctcaaagctg gccatgatct gctcccgctg
      541 cccgggcggc agttcagcct ccgcgaacga cagcacgccg gaggtgtact ttttcgcata
      601 cggcgaggca tcgataagct cccggacttc ctccggtttt ccctgcagca cggtcgcccc
      661 ctcgcgctgc cggtcttttc ccagcaggta atccaccgga ccggcaccac caccgcgccc
      721 ccgggggtga aatttaacga tcatcatccc cccctttttc cagcaccgca tgccgcagcc
      781 gctccagtcc cgcgtcgatt gccatcaggg cggccacaat ctgcacccgg tcatgaccgg
      841 caccaccggc attcactttc cgggcgatct ggttcaggtt gttccccata ccggcaagct
      901 ggcgcagcag cgccggcgat atcgacggca gtctgccggc acgcgccggc ttctcatcga
      961 ggcaggtctg acgcatccac gccgccagct gcctgccgtc gcaccgctcg agcagccgcc
     1021 ggtgttcgtc ttccgtcacc cacatcgtga gcatcttgtt gcgtttgtct gccagagtag
     1081 cctcctgaga acaccgggac gggcgggaaa tgtttcccgg tcggcacggt gttggacaag
     1141 ccgcaggcgc gtcagtggct tttagcgggt ttcggggcgc agccctgaac cagtcacgta
     1201 gcgctagcgg agtgtatact ggctcaatat gttacggagg agtaacactt tatgaaagtg
     1261 cttcatgtga cagggggaaa atgcagctac agggcgtaca ggagaaaatg gaggttacgg
     1321 tgatatgacg cctcctcgct cactcggtcg cttccgctcc tgccgttcgg ctgcggcggg
     1381 cgttacgcac acacggataa gagcaaaggc atatgacatg acaagcaggg cgggaaaagg
     1441 atgcggcgct gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1501 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1561 gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1621 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1681 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt ttccggtaac
     1741 tatcgtcttg agtccaatcc ggaaagacac gcaaaaacgc cactggcagc agccactggt
     1801 aacaggattc acggagcaga gtagtagagt gcttctacag agttcttgaa gtggtggcct
     1861 gactgcggct acactggaag gacagttttg gtatctgcgc tccgtgaaag ccagttacct
     1921 tcgggaaaaa gagttggtag ctcttgattc cggcaaacga accaccgttg gtagcggtgg
     1981 tttttttgtt tgcagggcat gagattacga cgagagtaaa aggatcacaa gaagatccgg
     2041 ctgtttacca gtgatttggc ttcagtattg gcttccgcct agtggggact ttatctggca
     2101 ataaagctaa tcttaaggct tcataggata catatggcaa gctccaatcc cctaaactag
     2161 ccagattact aatcactcga ctagtatcct gttcccttgg cactacgatt cctattttta
     2221 actcaggccg atcagctttt atcatttcta ttggcggtat catatctgag tcgttagtgc
     2281 agagaaccaa ttgttctact tcaccacgaa tcgcatcgcg atacatttct aaagccattc
     2341 taacgtctgt ctgcttttct tctaacttcc agaccgttgt tctatccgtt tttgatggag
     2401 gatgcccctc attaaaggtc attgcacatt cctgacgctc tgagtaataa ccttttatta
     2461 tctgtagctt atcgccatag atctgtacta atgctcgatg gtaggcttgc tgagcttggt
     2521 gcgccaattc accccgagaa gcaaactttg ccttgatctc agcagtaaag aacttcacct
     2581 gaatcaactg tgattctcgt gctcttatcg gaagcagttg ttcttcaaag agcttataaa
     2641 ggtctaacca cttatatccc gaacgcttaa ccaatccgtg ataaaaattg aacccatcta
     2701 cgtaaactat tgttctcatt ttatttcgcg cataaaaaaa ccgcctctaa gggcggtttt
     2761 aacaacaaaa actaacgtta gcaaacagtt taagttgtag tacctcaggg ttaaaactaa
     2821 ccgagagggc ggtttcaagc tcctgaagga agacgcgtaa cttaatcgct gcccacctaa
     2881 agggaggttt agagcctcaa gggagctgcg taacttaatc gcgctaccaa gaggtgagtg
     2941 tagattaagt atagttaaat ataatgatat tataagcgca ctggtgtcgt aataattata
     3001 tgaattaatt taccaacctg ctaagctgaa gtcttctaag aagacttcag cttagcaggt
     3061 tggtatatgt cagatcaaaa aaataattta gacattcaaa aacagacact tgagtctaaa
     3121 tgggggaagg aagcgttaag catgggatgg tcagccattc ctacatccct tttcttcctg
     3181 cagggcgcac tcaatatgac ccccacaaca ttcaatgtgc tacttcatct aattatccac
     3241 tggtggaaac cctatgattg gccacatcca tctcaagaga gcatcgcggt tagaatggga
     3301 gtttcatcaa gaacagttca acgctcactc caagaacttg aaaattacgg attgcttaca
     3361 aaaattcgta caagcaagga caaccctaag tacaaagggc gaaacattta cgatatgtct
     3421 cttttagtta agtcactaaa cattcttacg ccagacctca acgccaaact aaacgaaaag
     3481 taaccaaaaa ttccgacagc caaagcgcgc acatcactgt acaaaaggtg ttcagcatca
     3541 catttctaga aaatgacaac agctccctat tgaaagctaa tttctaaact aaacatcagt
     3601 atcgctacac aaacagcaat acggacaaaa cacaaaaaga ggaattagca tgtctaaagc
     3661 accaattggt acgactgctt ctaccggaca aaagtgtcct gaaagtggtg tctggaaagt
     3721 cgtaggaacc ccttccacta cagccccgat agcaaaaggt aatgtaatgc ctccgtacgc
     3781 aggaaaggct gtaacctgga tgttaaccca atatgcttga tatttagact ggcaacgaaa
     3841 agccgcccca gcgggcggct tttctatctc tgccctataa attcagaaaa ggaaagctaa
     3901 atggctgcat ataccagcga aacagagaag tttttacaaa gcgtcagtag cgatcaaaca
     3961 tggtggaagt cacgatataa tccaggagac agcgttcccc attctggaat ttatcgatgt
     4021 accgtctgcg gaaaagagat caccagtaac ggcagcgacc cgttcccgcc gcaaaaccat
     4081 catcagcaca gtcagcaaca agcaatcaaa tggcaattga ttgtcagaac ggacactaaa
     4141 ggtgaccgtt tcggaatcaa atgattgaga gccctccggg gctttttttg tacctgaagc
     4201 tcgaagatat ttaccataac atcccttgcc cgcaccgctc cggacaggtt cgccacaaac
     4261 gggacggcag tcaggtaaaa ccggcaacaa accacccgat acaccgcccc gttcgtgaca
     4321 gataaatttt ccccggctgc atggtcatgc agccagatgc atacagtctg atttcaggcc
     4381 agaatccgga tgaacgcctt aaaaacagga aaatgcttct ccagccagtc ccacagctcg
     4441 gtcgccagat acaggcagtt aagtacagct tcaatgacgt taagcaattt cataattccc
     4501 cctaggtata ggccgtgctc tttttgatca cgaacctatt tagcgggaga aataagagcc
     4561 cttcttcgtg cggcctgtca ccggatgata ctgttcagcc cagtgtcgga ccgtcataac
     4621 tccgctgacg ggaacgggct tccgccagct ctttttcgtg ctgtcgctgc tcgtatgccc
     4681 gttcggcggc caccgccatt tcatgccgct gcactactgg ctcaaacgct gtaactgcct
     4741 gctcaagttc gccacctgct gactcaagcg cgtcacgcgc tcgttcagtg ccgcgttctc
     4801 ccgttgcata agcccagaca tcttctgcca ttccgcgaag gcgctctccc actcgtccag
     4861 cctttgcgag tagtcctgat gtagctgctc taatgcggtc agcaactgtt tttccagttc
     4921 cgtcatgggc tatttcctct ccagtaccat ccagttcccg tcctccccgt aagctcccgc
     4981 tttcgggttc actttgacgc aggacagttt cttctccccg cagagcgaca gctgcacgcc
     5041 gtggttcttc tccgacagca tggcctgcgt ccgctcctgc gcccggatag tccggtaatt
     5101 cccggtgatc tgctgcccct gccaccacag aatgctcccg ctcgtggcga tcagcagtac
     5161 cgagaccagg gtgatggtca gccacgtccg gccgaccata cgcagcacgc tcaggcggtt
     5221 cgactgcatc gccgcggtca taccctgctc gtgggcgctg atggcgtcgc tgattctctt
     5281 cgcgcttgcg ctcagttccg cttttacaga cttctcgtgc tcgctgaatg cggctttcag
     5341 catctcgccg gtactctgc
//
