LOCUS       NZ_CP083005             5463 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 6712.08 plasmid p5-6712.08, complete
            sequence.
ACCESSION   NZ_CP083005
VERSION     NZ_CP083005.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016529
            Assembly: GCF_021137715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.2725x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 03:13:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,607
            CDSs (total)                      :: 5,487
            Genes (coding)                    :: 5,358
            CDSs (with protein)               :: 5,358
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 56 of 129
            Pseudo Genes (incomplete)         :: 64 of 129
            Pseudo Genes (internal stop)      :: 28 of 129
            Pseudo Genes (multiple problems)  :: 15 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="6712.08"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5-6712.08"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern, Friedbuehlstrasse 51, 3001 Bern,
                     Switzerland"
     gene            complement(join(5219..5463,1..289))
                     /locus_tag="K9F30_RS27900"
                     /old_locus_tag="K9F30_27860"
     CDS             complement(join(5219..5463,1..289))
                     /locus_tag="K9F30_RS27900"
                     /old_locus_tag="K9F30_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168774.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVILED
                     KLAEKPVTGKNRKN"
                     /name="hypothetical protein"
     gene            complement(292..1218)
                     /locus_tag="K9F30_RS27905"
                     /old_locus_tag="K9F30_27865"
     CDS             complement(292..1218)
                     /locus_tag="K9F30_RS27905"
                     /old_locus_tag="K9F30_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001736644.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1395..1793
                     /locus_tag="K9F30_RS27910"
                     /old_locus_tag="K9F30_27870"
     CDS             1395..1793
                     /locus_tag="K9F30_RS27910"
                     /old_locus_tag="K9F30_27870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(2054..3073)
                     /locus_tag="K9F30_RS27915"
                     /old_locus_tag="K9F30_27875"
     CDS             complement(2054..3073)
                     /locus_tag="K9F30_RS27915"
                     /old_locus_tag="K9F30_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(3874..4998)
                     /locus_tag="K9F30_RS27920"
                     /old_locus_tag="K9F30_27880"
     CDS             complement(3874..4998)
                     /locus_tag="K9F30_RS27920"
                     /old_locus_tag="K9F30_27880"
                     /inference="COORDINATES: protein motif:HMM:NF013211.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_021529456.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFISGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="colicin-like pore-forming protein"
     Typing          complement(2299..3073)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.392"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(296..1218)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcagagcatc cgcgtttgcc ttcatgtttg cgactgactc ttccagccgc tgaaaatcct
       61 ggctgatgcg ctcacctatc agccctgcta cgtccagatt ctgcacctca tttctgactt
      121 catccctgac ctgttttgcc acaccgtcag aaatatcccg atatccgcga agtgtttccc
      181 ttgccacagc cagagaatca gcaacagctt tctgttgctc cattgcggta tgttgcaaat
      241 gctccaccga taacaggagc gtgagtaatt tttcttctgt gctggccatg ttcagaatcc
      301 catagaaaat ccacgtgacg gtgatttaac ccgttcaggt tcagttcggt cctgtctgct
      361 cactctgtct ctcatgtcaa agccatgctc ccgcgcaaca tcatgttgca gccttctgac
      421 cagttcatgt tgcttaaccg gagtgtccag ccattccatg atgtcatgcc cgaccgttcg
      481 cgcctgttca agatggcggg aatagtccgg ttcagaccgt ttttgcatga tgtcatgacg
      541 ctgggcatca tcaagccgtc cggcagcttc aaaacccaga tgcacttcgg gacgtcgttc
      601 aatgccctgt tctttcagcg tccgacagtc aatgaagccc ctttcaaagc cattttttct
      661 catatgttcg ttgtgcttca cctcccagcg ttttcgcaat tcccgcaact gctctttttg
      721 ctgagtggga gtcaggcttc ctgaatcctt tttcgcacct ccccgctcgg gatacctggc
      781 gttatagcgc cggaaatact gttccggtgt acgttcaatg ccatcattca cacgctgact
      841 catcatgata tgcgcatgag gctgttcgcc tccctcaatg ctcgctttcg gattgtgaat
      901 ggcaaaactc catgcatgcc gttcgccagc ttcctgacgt acaaaatcct gcaccagttc
      961 aaggcgctgt tccggtgtca gttcacgggg cagcgctatc tccagttccc gataagtcga
     1021 accgtttgca cgttcaaact ggtcagccgc ctgccagaaa tgggagggat tatctcgcgc
     1081 ccattccggc atgtttccgt gtgcggaata ttccaaatct tgacggtcgc ggtatttctc
     1141 tttgcgctcg atgtaatcgg catggtttgc agcctgtccc ttgccaccaa acttaacgct
     1201 gaggtgatag gtcgccacgg tttcagccct ccgcaggagc gcactgtttc tcgacgaagg
     1261 agagaaacat ataagtgcgc ttccttctaa ttttacgaat tagcacggaa gataccccct
     1321 gcacccgccg accgcaagcg atatactgca cgaccacaac acaccgcaaa aaaacaccag
     1381 ggagcaccac gaacatgcgc atcagcaaca tcgaatggct gaagaaacgg atcggattca
     1441 tcaggaagct cggagaacag accgcacgac agagacagat tattgacctg ctcgacaacg
     1501 aagcaggact cacagaacag gaacgcaaac tcctccacgt actggcaaca gcggaaaaaa
     1561 acgacttaca ggcacaggag agcgaacgaa aacaggcggt ccaaaaacga atcgagggaa
     1621 aaaaacaacg acgagaacgc aatcaccgac tctttctcgc ggctgggcta ctgatcgaag
     1681 caggactggt agacacaaaa acaggcgagc tatgctataa aaaagacagg atattacagg
     1741 cacttaagga aattaagtac gatctggaga catccccaaa cccagacgcg taagtgcctc
     1801 catccgtgtt ccggttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga
     1861 gactgcggcg agcacgacca cctcaatcat gcggtaagcc gcaccattgc tgggctgaga
     1921 ggagaaaacc gccggaaagc ccgctgctca aaaagacgcc gaaaggcgct gttatctgat
     1981 ataggcttgt tcatgtccgc gaatttaaca tcccattaac aaaaaacggg gggtagtgtt
     2041 gcttctttgt taactaacat ttaattattt gtttcttggt tagccattct ccagaattat
     2101 ccatccagcg tcgaatagtc cggctgctaa caccaaccgc cactgcaata tcttccgttg
     2161 acgctcccaa gatatacata tcaagcgccc tacgacgttt gtcgtcgtta gcagtccacc
     2221 gaacagcggc gctttttttc cctttgtccg actgattttc tgacgaattg gccttcccgc
     2281 ccttgcgacc gcgtgctgcc tgaatttctg gcgtgtgcgt atcagccaca tactgtgcga
     2341 aagtttccgg cgtgaagttc ctgtgcgtgt atttcgcaat gctcttgcca atagcccgac
     2401 attcagccag tgaaagcgga acgggaagcg atgcattgta catttcgaca cgctggatca
     2461 ccgcttcaag ccactgtgag aacgctggcc agccctgacg aatcgccctg taagcccatt
     2521 tgcgcgtcat ttcgaacagg tggcagtttc gccccatccc gtaatgttta tcgatgctac
     2581 gacgctctga ggcgctcaaa tcgagataat ctgccagttc atcgagggta taggcgtcct
     2641 cgcgccactc catcacctgc cattccaggt ggaacggatt tttgcaaatc aggccgctgt
     2701 agttcacatc agcacaaagt ttctcacaca gcgaacgttc aaccgctgcg gcatatttca
     2761 gcgccttaac cgaggcatca ggcgcggttc tcacggcgat gttgagtgcg tagagcaggt
     2821 gagcgtgtcc gttcacagga tttttaacgg tgatgttcgg cgcgggggcg ttccggtcgc
     2881 tccagtcaat cgcagccccc tcgcggtcaa cgtcaaaaac cagccagaac tgtgcatgag
     2941 gctggttctg ctggatatac cgtgcaagaa gcgcgcgccc tttgccggag atacgaaggc
     3001 cgaaagcgag atcatccgtg tgatagggac ggtggggtaa attttcttcg aagtattgaa
     3061 gcgcggcgct catgtgttac gattccttcc aggttatcga ccgacatcgc ccctgtattt
     3121 tcgtgaaagt cttggcggat agacacgaat ttcaggggcg tttgtttttc agtcgcttac
     3181 actaccccca acgacaaaac ctgtcgagcc ttcccgcaaa tccgatccat ttaccataac
     3241 tcccgttgta cgtacccctt aaaacgccct cagtcgcttt ctggcgcaaa aaacgcaaaa
     3301 agacaaggaa acaccaggcc aaaccagaaa aatgcgtcag aatcactttc agggcgttgc
     3361 ataactatgc acgaaagcga ttcgcaaaca gtttcgaccg ttcaaaaggg cgaacgagtc
     3421 aaaaaagtaa cttttgtgat aactacttct agatttatgg ataaagtaag gcaatattat
     3481 aaacatttaa ctaacgagga ttttataaat gaaaaaaata gatatggaat tctatttaag
     3541 gaattttatc cttgccttat ccttttacat tttatctttt atcatcgcaa gtttgaatct
     3601 aagcccaaca agtatagaat tatttatatt tatacttata tctctttttg gatcgtccac
     3661 ataccctttt tgtctattac taataaataa gcaagcaccc ttactaacaa aaacaacttt
     3721 taacttcgtt aactggggtt ttataatatg cattatatta tcactacctc catttggatt
     3781 gatttttaca gtctattact ttataaccaa caaaaagcga tagaaaaaca aaataaaggt
     3841 cataccatat caaacggtac gaccttttgt ttattaaatg ccgttaataa agctgtttag
     3901 ttgtttcagc ctttcatctg taattaaggc actagtcact gccataatta ctgcaaaccc
     3961 taaaatacca ataggattta ctgccatagc actaaatgcc actgtaacca taacagatgc
     4021 taccttgccg actgcgattt tttccccgct tataaaagcc tcattccagt caccagttct
     4081 gaatccttta actaagccac tgataaatcc cccccactga atagcctcac ctacgacacc
     4141 aaaggcctta ccaaaaattt ttaactgttt agccatttga tctttattaa gagactccag
     4201 tgcttttgca atcgcttctc ggtcggatac gctgaatttc ttattaagca catccttgta
     4261 cctatcaaag gctttaatcg cctcattagc attgcgaatt tttttccctt tagcttcatc
     4321 ggctagtgcc ttggcttctt tctccaactt atcgccgtat tttgatgcta actctttata
     4381 aaagtcatta actttgctta ttgcatcatt tacagcatta gcttcatttt ctgccttgaa
     4441 ttctaaaaag ctttttatag tatcacgagt cagtttatta cctgatgacg gcccactacc
     4501 gatattatag tgaacattca ttttattcac atcaccgttt ggtacagtta ctgtagctac
     4561 tgcaccacca gtataatgga tagtgtacgt tccagaatgg tcatctattg actgaataag
     4621 tcccttaatt aacttatctt tatttgtcgg taaatatctg ccaggtttcc cagagtcagg
     4681 gctccatcca ggaatagacg ttgtttttcc attgccgcct gaattgctgt tatgatttcc
     4741 agcaccgtga ccattacctg agccaccatt accgtgatgt tttcctggag gatctttttc
     4801 cataccaccg gtaatgttga tatgcacctc cccgtcagga cctacagtgc ttgcaggacc
     4861 ttttcccgga ttgtaatgat acccaccgcc agatgatggt cctccatgtc cagatgagcc
     4921 attcacgttt ccactgggtt taccaccgtt atgtccaagc cctgtattac cagggccacg
     4981 accatctcca ccactcataa aaattccctc ttttaatcgt taaaacaatt aattacatag
     5041 ttaatactgt acataaaacc actggtttta tgtacagatc aaaaagtact cgccattatt
     5101 ttttctgtca agtcagggag ggatattttt tggcagttcg gaggaatcac tcatgaaaac
     5161 ggtgaacgga acgcgaaatc ctttcgctgt ccgtgaggcg ttggacaagc gaagcgcgtc
     5221 agtttttccg gttcttaccc gtgacaggtt tttccgccag tttatcttcc agtatcacaa
     5281 ctttctgata caccgccgct gtggcgttgt agttcctgtc gagcattgtt ttcattcccc
     5341 atccgagcac acagaacata actagaaaag ccactgacac cccacccagc catttccagc
     5401 tatacccttt aagggtgttg tattcctgca cgatattgct gtgaattgtc gcaatatccc
     5461 tgt
//
