LOCUS       NZ_CP083004             5598 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 6712.08 plasmid p4-6712.08, complete
            sequence.
ACCESSION   NZ_CP083004
VERSION     NZ_CP083004.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016529
            Assembly: GCF_021137715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.2725x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 03:13:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,607
            CDSs (total)                      :: 5,487
            Genes (coding)                    :: 5,358
            CDSs (with protein)               :: 5,358
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 56 of 129
            Pseudo Genes (incomplete)         :: 64 of 129
            Pseudo Genes (internal stop)      :: 28 of 129
            Pseudo Genes (multiple problems)  :: 15 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="6712.08"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4-6712.08"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern, Friedbuehlstrasse 51, 3001 Bern,
                     Switzerland"
     gene            complement(join(5390..5598,1..37))
                     /locus_tag="K9F30_RS27850"
     CDS             complement(join(5390..5598,1..37))
                     /locus_tag="K9F30_RS27850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     gene            288..797
                     /locus_tag="K9F30_RS27855"
                     /old_locus_tag="K9F30_27810"
     CDS             288..797
                     /locus_tag="K9F30_RS27855"
                     /old_locus_tag="K9F30_27810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            804..1016
                     /locus_tag="K9F30_RS27860"
                     /old_locus_tag="K9F30_27815"
     CDS             804..1016
                     /locus_tag="K9F30_RS27860"
                     /old_locus_tag="K9F30_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1392..1520)
                     /locus_tag="K9F30_RS28005"
     CDS             complement(1392..1520)
                     /locus_tag="K9F30_RS28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            1683..1907
                     /locus_tag="K9F30_RS27865"
                     /old_locus_tag="K9F30_27820"
     CDS             1683..1907
                     /locus_tag="K9F30_RS27865"
                     /old_locus_tag="K9F30_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1891..2157
                     /locus_tag="K9F30_RS27870"
                     /old_locus_tag="K9F30_27825"
     CDS             1891..2157
                     /locus_tag="K9F30_RS27870"
                     /old_locus_tag="K9F30_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2227..2577)
                     /locus_tag="K9F30_RS27875"
                     /old_locus_tag="K9F30_27830"
     CDS             complement(2227..2577)
                     /locus_tag="K9F30_RS27875"
                     /old_locus_tag="K9F30_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2643..3188)
                     /locus_tag="K9F30_RS27880"
                     /old_locus_tag="K9F30_27835"
     CDS             complement(2643..3188)
                     /locus_tag="K9F30_RS27880"
                     /old_locus_tag="K9F30_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433973.1"
                     /translation="MFFKRYLTEKEIYKVALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3214..3540)
                     /locus_tag="K9F30_RS27885"
                     /old_locus_tag="K9F30_27840"
     CDS             complement(3214..3540)
                     /locus_tag="K9F30_RS27885"
                     /old_locus_tag="K9F30_27840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197760.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINEMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3900..4058)
                     /locus_tag="K9F30_RS28075"
     CDS             complement(3900..4058)
                     /locus_tag="K9F30_RS28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4698..4874
                     /locus_tag="K9F30_RS27890"
                     /old_locus_tag="K9F30_27845"
     CDS             4698..4874
                     /locus_tag="K9F30_RS27890"
                     /old_locus_tag="K9F30_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165764108.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSV
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4864..5208
                     /locus_tag="K9F30_RS27895"
                     /old_locus_tag="K9F30_27850"
     CDS             4864..5208
                     /locus_tag="K9F30_RS27895"
                     /old_locus_tag="K9F30_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004197764.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4532..4612
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.476"
                     /coverage="98.0"
                     /evalue="3.1e-22"
     Typing          complement(3950..4347)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.801"
                     /coverage="91.0"
                     /evalue="6.52e-141"
     Typing          3..1132
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.735"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5199..5596
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.19e-79"
ORIGIN
        1 gtggcagacc gtggtgaacg ggcgactggg gctgcacgac ccgaacgcac cggagaaccg
       61 gcgcgcgctg gtgacgccat cttcgttacc caaaacgaag caggaagccg cagaggctat
      121 tacgcggggc ttactggccc tcgcctcgtc aggggagctt aaaacgcgtc aggacgtcac
      181 tgaggcgctg gaaagcgcag gttttgaggt cgtgcgcacc acaaagagca gcatcagcat
      241 tgccgacccg gacggagggc gaaacatccg acttaaggga gccatctatg aacagtcttt
      301 taacgctggc gaaggactta gagcagaaat cgaaagcgca gcagcagagt accggcgaaa
      361 tgctgaaagc cgcattcagc gagcacgaga agtctgtcag agcggaactg agcgaaagcg
      421 ggaagagaat cagcgccgcc atccgcgccc acgagcaggg tatgagcgcg gccatgcagt
      481 cgaaccgcct gagcgtgatg cgtatggtcg ggcggacatg gctgaccatc acgatggtgt
      541 caggactgct gttcgccagc ctgagcgggg tgttgtggta tcaggggagc ctgatagcgt
      601 caaatctggc cgaaatcgac cggcagaacg cggcgctgtc gaagctgaac gcgaagacct
      661 ggggcgtgac gtacctggaa gacagcaacg gacgttttct ggtgctgccg aaggggacgg
      721 ccgtagacag gacgcagagc tggacggtcg ggaacgggcg gtcgaagcag aacgcgctga
      781 gactggtgaa ggagtaagtg ccgatgaccg agctggaaaa acagttgctg agcgcattag
      841 agcagctaca gcaggactac tcgaaaaggc tggacgagtg ggagagcgcc ttcggggaat
      901 ggcggaaaat gtcagggctt atgcaacggg agaacgcggc gctgagcgag cgcgtgacgc
      961 gcttgagtca gcaggtggaa cgcttgagcg ggcaactgca gcgtttgagc cggtagtgca
     1021 gcgtcatgag atggccgtgg ccgcagaacg ggcgcacgag caacggcagc acgaaaagga
     1081 actgacggaa gcgcgctccc ggcagcgaag ctatgacggt ccgtcgctgg gctgaaatgc
     1141 aggaaaacag ggttctcatc gggcatttac gctgaaaccg gatgtcattc acctggctgc
     1201 atggctatgc agccaggtaa aatttatctg tcgcgaacgg ggcggtttat cgggtggttt
     1261 gttgccggtt ttacctgact gccaccccgt tcgcggcgaa cccgtccggg gcggtgcggg
     1321 caacgggtgt tatgtttaat cagcgtgaac ggcagataca aaaaagcccc ggagggcttt
     1381 taactactga cttatttttt tgaagatttg ctgggtttca atttgtcagt atttcccggc
     1441 gtttttttat tgtcacgctg gcgtttatca ggttcccctg ttgaatcggc aattcgtttc
     1501 ggacccggct taagacccat aatcgttatc ctttcaatag ttaagaggaa agccctcaag
     1561 ctagatatat tgaccgcgtt acggattatc aagaggggct aacaagatta ttttttgttc
     1621 ttttaggttg attgtggtac tgttgatata catgtatagc aaatggtgtt cgggaggttt
     1681 atatgcttgc tatccggtta tctgatgaga ttgagtcccg tctggactcg ctggcgaagc
     1741 aaaccggcag aacaaagacg ttttatgcgc gggaagcaat actggcgcat ctggaagacc
     1801 tggaggatta ttatctttca gcagaaactg ctgcacgcgt tcgccgtggt gatgaagcag
     1861 tgcattcgtc tgaagacgtg aggaagtcac ttggtctgga cgattaacta ttccgatcgg
     1921 gcgctcaaat cgttacgcaa gatggacaaa cagaacgcac gacggattgt ggattttatg
     1981 agtttacgca ttgcagttgc tgccgatcct cgccagtcag ggaagccgct caaaggtgag
     2041 ctgggcgagt tctggcgcta tcgggtggga gattatcgcg ttctgtgtga gatccgagat
     2101 gacgagcttg ttatccttgc cgccacgatt ggacatcgcc gcgaagttta cgactgaagc
     2161 ccgctatgcg ggcttttttg atgccgacaa caggtattat ggtaaatact cgactaacta
     2221 cgtgttctac tgtttgtcgg ttttagttaa ccagcaggtt gtcttaacga taatgtagcc
     2281 gttattttta actaataagg tcatattgtt aacgtcaggc ataataaaag acattatggc
     2341 ctcagagtta tcatcagaag cagctctctg cattactact tcgattccct tttctttgaa
     2401 tctataagta tccagttgcc tattaaactt agaaagacct acaaccatac ttagtttgtt
     2461 atctttaaca gtaagagatg cttcctgagt accattacat cggtagtaag cggtgtaacc
     2521 gtcagcaaaa gatcgaccgg ggaatataaa tgctaggaac gcaattaacc aatacatagt
     2581 tgagaatctt ggtattacac atttcactat cctctctttc atttttcgtt caccatactt
     2641 tgttatctga tttttaattt ttctatttta taaaccaagt ccctaccaaa aaggcgaaaa
     2701 ttatcctcaa ataataataa ttgtttaaga acatccatga aagcagctgc ttcaccctca
     2761 gttatgggag ctactctata taacatataa cctgagttaa gggtttcttt tatactttct
     2821 tcaatgctca ttataaatat tagtggatta tcatttttct tcccccatgg gtttctaatt
     2881 aatccttcga tgtactcagg tcctttgcgt cctattgcct gcgcaatgtt ttttatttca
     2941 tctgcgtgag ggctttgttg tacagctttg tttatgcctg cttgagtgat tttcagagac
     3001 tctacaccgt gtatgttgta tgagataaaa tgtaataaat taagatgtaa tgcaattaat
     3061 ccatttttgt ttacatctga gtatttggat aaaaaacgta atgaagtgtt aactctccct
     3121 tgaagcctaa caattgggag tgctaccttg tatatttcct tctccgttaa ataccgttta
     3181 aaaaacataa ttctccgcct ccgtatagcg tgcctatttt atttcgtcgt aaaacttagc
     3241 tctgaaatat tcctttagaa ccatcatttc attaataatt gaatctatct catccatttt
     3301 ttcttggtat tcttttaatg gtgaagggag cggtaactca atatttgaaa agtgattggc
     3361 gagataggga tcaaggtttc tcatccattc aggcttttcg ccaatgcgtt cttcaactga
     3421 aagtgaacct gataatctct ggttaataag atcacttaga ctcattttgg ttttattcga
     3481 aagccgttgt aaggattgct ttaatcctat atctagcatg atatttgttc tttctctcat
     3541 tacccccccc ctcgctattg cgttttttgt tgcgcacaaa gctaccattg cgcattaaga
     3601 aacgcaataa aaaaggaggg agggagtggt ttgtacatga tgtgcagtgc gctgagggca
     3661 tcagtggcgg gtgtttttcc gtgggatgga tggagctccg gggataacag aactgacgat
     3721 catttaaagg atcttcttga gatccttttt ttctgcgcgt aatctcttgc actgtaaacg
     3781 aaaaaaccgc ctggggaggc ggtttgatcg aaggttaagt cagttgggga actgcttaac
     3841 cgggtaactg ggtatacgag accgcagata ccaaaaatgt tctttcagtg tagccgcagt
     3901 taggcaccca cttcaagacc tctcaatatc tctctcgtat atatccagtt accaatggct
     3961 gctgccagtg gcgtttttgc gtgtccgtcc gggttggact caagatgata gttaccggaa
     4021 agggcgcagc agtcgggctg aacggggggt tcgtgcatac agcccagctt ggagcgaacg
     4081 acctaaaccg agccgagata ccagcagcgt gagctatgag aaagcgccac gcttcccgaa
     4141 agggaaaaag gcggaacagg tattcggtaa gcggcagggt cggaacagga gagcgcacga
     4201 gggagccgcc ggagagaaat caccggcatc tttaagtcct gtcgggtttc gtcctcctct
     4261 gacttgagcg tccatttctg tgatgctcgt caggggaggc ggagcctatg gaaaaacggc
     4321 cacgccgtat ccttttctcc ggcactttct gatttgagcg cctttgcttt atcccgttat
     4381 ccgtgagccg gtcacgcccg ccgcagccga acggcaggag cgcagcgacc gagtgagcga
     4441 ggaggcgtca tttcatctgt ccgtgcatgg tattgcggac gctcctgagt cacatatcac
     4501 acctgccaca tgaggcactt tcatgaatct cgctctcatg ccaacatagt aagccagtat
     4561 acacagcgta aacgctgtga ctggttcagg gctgcgcccc gaaacccgct aacaccactg
     4621 acgcgctggc gcttgtccaa cgccggcgcg tcccggaaaa catttccgtg ccgtccggcg
     4681 tttgcgaagg agggataatg cagaagccga agaagaagcc gctcgtcgta aaaaaaagat
     4741 cctggcatcc ggcagtgatg gcaaatctga tgcgtcaggc acaggaggaa gattaccgcc
     4801 gggagcagat ggcagagctt gagcgttcag tccggtcaca ttttgcggat atcggaggag
     4861 aggatggcgg ttaagcgtac ccgtttttta ggtatccggg tgaccgatgg ggagtaccag
     4921 cagctgctgg agcgctgcaa tggaaggcaa ctggcggtgt ggatgcggga aacctgtctc
     4981 gatacgcgcc cggcgcggtc gttgcggctg ccgtccatcg atccggtttt gctgcgccag
     5041 cttgccggga tggggaacaa cctcaaccag atcgcccgga aaattaacgg cggccagtgg
     5101 tcaggcgctg accgggttca ggtggtggcc gcgctgatgg ccatcgatgc cggactcgag
     5161 cggctgcggc atgccgtgct ggaaaagggg gcagacgatg atcgttaagt ttcatccgcg
     5221 agggcgaggc ggcggtgccg ggccggtgga ttatctgctg gggaaagacc gccagcgcga
     5281 cggggccatc gtcctgcagg gaaagccgga ggaggtccgg gagcttatcg acgcctcgcc
     5341 ctacgccaaa aagtacacct ccggcgtcct gtcttttgct gaacaggatt taccgcccgg
     5401 ccagcgtgaa aagctgatgg cgagcttcga gcgggttctg atgcccggac tcgataaaga
     5461 ccagtacagc gtgctgtggg ttgaacaccg ggacaagggg cggctggaac tgaattttct
     5521 ggtaccgaac acggaactgc tgaccggcag gcgtctccag ccgtactacg accgggcaga
     5581 ccgtccgcgc atcgatgc
//
