LOCUS       NZ_CP082983             4052 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain BD-13 plasmid p5-BD-13, complete
            sequence.
ACCESSION   NZ_CP082983
VERSION     NZ_CP082983.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016508
            Assembly: GCF_021137635.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 662.1413x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 03:12:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,740
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,468
            CDSs (with protein)               :: 5,468
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 61 of 153
            Pseudo Genes (incomplete)         :: 87 of 153
            Pseudo Genes (internal stop)      :: 26 of 153
            Pseudo Genes (multiple problems)  :: 16 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082983.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BD-13"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5-BD-13"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.9321 N 7.4199 E"
                     /collection_date="2018"
                     /collected_by="Labormedizinisches Zentrum Dr Risch AG,
                     Waldeggstrasse 37, 3097 Liebefeld, Switzerland"
     gene            join(3953..4052,1..386)
                     /locus_tag="K9F21_RS28565"
                     /old_locus_tag="K9F21_28540"
     CDS             join(3953..4052,1..386)
                     /locus_tag="K9F21_RS28565"
                     /old_locus_tag="K9F21_28540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            390..602
                     /locus_tag="K9F21_RS28570"
                     /old_locus_tag="K9F21_28545"
     CDS             390..602
                     /locus_tag="K9F21_RS28570"
                     /old_locus_tag="K9F21_28545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1110..1568)
                     /locus_tag="K9F21_RS28575"
                     /old_locus_tag="K9F21_28550"
     CDS             complement(1110..1568)
                     /locus_tag="K9F21_RS28575"
                     /old_locus_tag="K9F21_28550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            2934..3275
                     /locus_tag="K9F21_RS28580"
                     /old_locus_tag="K9F21_28555"
     CDS             2934..3275
                     /locus_tag="K9F21_RS28580"
                     /old_locus_tag="K9F21_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2668..2847
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(2205..2685)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          6..718
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.639"
                     /coverage="99.0"
                     /evalue="5.12e-145"
     Typing          3239..4032
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.189"
                     /coverage="95.0"
                     /evalue="8.17e-155"
ORIGIN
        1 agaaatctgt aaaagcggaa ctgagcgcaa gcgcgaagag aatcagcgac gccatcagcg
       61 cccacgagca gggtatgacc gcggcgatgc agtcgaaccg cctgagcgtg ctgcgtatgg
      121 tcggccggac gtggctgacc atcaccctgg tctcggtact gctgatcgcc atgagcggga
      181 gcattctgtg gtggcagggg cagcagatca ccgggaatta ccagactatc cgggcgcagg
      241 agcggacgca ggccatgttg tcggagaaaa accacggcgt tcagctgtcg ctctgcgggg
      301 agaagaaact gtcctgcgtc aaagtgaacc cgaaagcggg agcttacggg gaggaaggga
      361 actggatggt actggagagg aaatagccca tgacggaact ggaaaaacag ttgctgaccg
      421 cattagagca gctacatcag gactactcgc aaaggctgga cgagtgggag agcgccttcg
      481 cggaatggca gaagatgtct ggtcttatgc aacgggagaa cgcggcgctg aacgagcgcg
      541 tcacgcgctt gagtcagcag gtggcgaact tgagcaggca gttacagcgt ttgagccagt
      601 agtgcagcgg catgaaatgg cggtggccgc cgaacgggca tacgagcagc gacagcacga
      661 aaaagagctg gcggaagccc gttcccgtca gcggagctat aacggtccga cgctgggctg
      721 acaggcttca ccagtgacaa accccacgaa gaagggctca taaatttccc gctaaatacg
      781 gtcgtgatga aaacatcacg gcgtatacaa cggagggatt atgagactgt ttaacgtgat
      841 tgagacggtg ctgaactgcc tgtacatggc gctggagatc tgggactggc tggagaagca
      901 ttttcctgtt tttaaggcgt tcatccagat tctggcctga aatcagaccg tatgcatctg
      961 gctgcatgac catgcagccg ggtaaaattt atctgtcacg aacggggcgg tgtatcgggt
     1021 ggtttgttgc cggttttacc tgactgccgc cccgtttgtg acgaacatgt ccggagcggt
     1081 gcgggcaaga gatgttatgg taaatcatat tagttctgaa tgattcgggc tccaccagtc
     1141 attactattt gagcaccacc tgacattttt atacttgtac cctggctagt cattgtcgta
     1201 tcaccttgta tgatcgtttt tccagtagca tgttgacctc caccagtgac taaaattgac
     1261 gcttgtactt gagatccatt tatcaaaaca gttccggctt ggtgatcaaa ttttattccg
     1321 ctcaatgctt ttaattcagc tccatctagc tgaatttcac cattggctgc tgcagcctga
     1381 atggccccct gcatctgaga aaataattta tcaacttcgt caaaaggtaa tggtgtttca
     1441 agcagtgagg cttgttgttc aacttcagga tgtttacttg ctaacttccc tatgatgcct
     1501 ttgattgcat cagaagtaag attgcttaat gcgccagtga gaacggcaag tagtgtggtt
     1561 tcaatcatta tatttccctc gttagattgc tgtgatgcgt attatttact ctttataatc
     1621 tccgattctg tccatatggt tagttgcact attacgttta taagtgccac ttttaccata
     1681 tatatatcca acgctacatt gtggacattt tggcgtctcg tcattatgag tgaaatttat
     1741 ttctcctccg acagataggg tttttacgtt ttccggataa tctaatgtag ccacgccggt
     1801 ccattcacaa ttcgagcaga caacatcgaa agatgttggg atactcttgc agttgatgtt
     1861 gatttgcata tgcataacct cgattttgca cagaaattac agtgttatta gttgcatacc
     1921 gactataacc tgagtttgtg atggagtggt gtttttttgc tttgacttga gtaagagaaa
     1981 gagatgatct tcttgagata atctctttct gagcgtagtc ttttgctctg taaacgaaaa
     2041 aaccaccctg gcaggtggtt tttcgaaggt tagttaatcc tggcagattt actaaccgtg
     2101 gtaacagtct tgtgcgagac atgtcaccaa agctgtcctt ccagtgtagc cgcagtaagg
     2161 ccaccacttc aagaactctg aatacatctc tcgcacatcc tgtttaccag tggctactgc
     2221 caatggcgat ttgtcgtgcc ttttcgggtt ggactcaaga cgatagttac cgaaaagagc
     2281 gtggtagtcg gactgaacgg ggggatcgtg catacagtcc agcttggagc gaactgccta
     2341 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
     2401 cggtaaaccg aatggcagga acaggagagc gcacgaggga gctaccaggg ggaaacgcct
     2461 gggatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag attttatgat
     2521 actcgtcagg ggggcggagc ctatggaaaa acggcaacgc cgcatcctct tcccggcttc
     2581 tcctgatttg agcgctgctt gcgtatcccg tcatctgtga gctggtaacg cccgccgcag
     2641 tctcacggca ggagcgcagc gactgagtga gcgaggaggc gtcatttcac ctttaccagc
     2701 atgttgcact tacgcccgtc agccacattt tccccttccc acatgaagca ctttcataca
     2761 ttaccactcc gccgtaacat atttagccag tatacactcc gctagcgcta cgtgactggt
     2821 tcagggctgc gccccgaaac ccgctaaaag ccactgacgc gcctgcggct tgtccaacac
     2881 cgtgccgacc gggaaacatt tcccgcccgt cccggtgtta tcaggaggct actctggcag
     2941 acaaacgcaa caagatgctc acgatgtggg tgacggaaga cgaacaccgg cggctgctcg
     3001 agcggtgcga cggcaggcag ctggcggcgt ggatgcgtca gtcctgcctc gatgagaagc
     3061 cggcgcgtgc ctgcagactg ccgtcaatat cgccggcgct gctgcgccag cttgccggta
     3121 tgggtaacaa cctgaaccag atcgcccgga aagtgaatgc cgttggtgcc ggtcatgatc
     3181 gggtgcagat tgtggccgcc ctgatggcaa tagacgcggg actggagcgg ctgcggcatg
     3241 cggtgctgga aaaagggggg gatgatgatc gttaaatttc atccccgggg gcgcggtggt
     3301 ggtgccggtc cggtggatta cctgctggga aaagaccggc agcgcgaggg ggcgaccgtg
     3361 ctgcagggaa aaccggagga agtccgggag cttatcgatg cctcgccgta tgcgaaaaag
     3421 tacacctccg gcgtgctgtc gttcgcggag gctgaactgc tgcccgggca gcgggagcag
     3481 atcatggcca gctttgagcg ggttctgatg cccgggctcg ataaagacca gtacagcatt
     3541 ctgtgggtgg agcacaccga caaggggcgg ctggagctga atttcctgat ccccaacact
     3601 gaactgctga ccgggaaacg cctgcagccg tattacgatc gcgccgaccg gccgcgcatc
     3661 gatgcctggc agaccgtggt gaacggcaga ctgggactgc atgatccaaa cgccccggag
     3721 aaccggcggt tactggttac gccgtccgca ctgccggaaa cgaagctgga agccgctcag
     3781 gcgattacac gtgggttgct ggccttagcc tcatccgggg agctcaaaac tcgcgaggac
     3841 gtcacaggtg cgctcacagc ggcaggtttt gaggtggtcc gcactacaaa aaacagtatc
     3901 agcattgccg acccggacgg ggggcgaaac atccgactga agggagccat ttatgagcag
     3961 tcttttagcg ctggcgaagg acttggagca gcaatcgaaa gcgcagcagc agagtaccgg
     4021 cgagatgctg aaagccgcat tcagcgagca cg
//
