LOCUS       NZ_CP082973             4052 bp    DNA     circular CON 10-FEB-2024
DEFINITION  Klebsiella pneumoniae strain BD-41 plasmid p6-BD-41, complete
            sequence.
ACCESSION   NZ_CP082973
VERSION     NZ_CP082973.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21016516
            Assembly: GCF_021135615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     A collection of hybrid assemblies of carbapenemase-producing
            Klebsiella pneumoniae clinical isolates from Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 528.8007x
            Sequencing Technology :: Illumina NovaSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/10/2024 02:48:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,786
            CDSs (total)                      :: 5,665
            Genes (coding)                    :: 5,500
            CDSs (with protein)               :: 5,500
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 68 of 165
            Pseudo Genes (incomplete)         :: 96 of 165
            Pseudo Genes (internal stop)      :: 27 of 165
            Pseudo Genes (multiple problems)  :: 20 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082973.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BD-41"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6-BD-41"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.9321 N 7.4199 E"
                     /collection_date="2018"
                     /collected_by="Labormedizinisches Zentrum Dr Risch AG,
                     Waldeggstrasse 37, 3097 Liebefeld, Switzerland"
     gene            complement(join(3975..4052,1..381))
                     /locus_tag="K9F23_RS28800"
                     /old_locus_tag="K9F23_28745"
     CDS             complement(join(3975..4052,1..381))
                     /locus_tag="K9F23_RS28800"
                     /old_locus_tag="K9F23_28745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            1747..2088
                     /locus_tag="K9F23_RS28805"
                     /old_locus_tag="K9F23_28750"
     CDS             1747..2088
                     /locus_tag="K9F23_RS28805"
                     /old_locus_tag="K9F23_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2297..>2728
                     /locus_tag="K9F23_RS28810"
                     /pseudo=""
     CDS             2297..>2728
                     /locus_tag="K9F23_RS28810"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2766..3251
                     /locus_tag="K9F23_RS28815"
                     /old_locus_tag="K9F23_28760"
     CDS             2766..3251
                     /locus_tag="K9F23_RS28815"
                     /old_locus_tag="K9F23_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            3255..3467
                     /locus_tag="K9F23_RS28820"
                     /old_locus_tag="K9F23_28765"
     CDS             3255..3467
                     /locus_tag="K9F23_RS28820"
                     /old_locus_tag="K9F23_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1481..1660
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(1018..1498)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          2079..3583
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtcattgtc gtatcacctt gtatgatcgt ttttccagta gcatgttgac ctccaccagt
       61 gactaaaatt gacgcttgta cttgagatcc atttatcaaa acagttccgg cttggtgatc
      121 aaattttatt ccgctcaatg cttttaattc agctccatct agctgaattt caccattggc
      181 tgctgcagcc tgaatggccc cctgcatctg agaaaataat ttatcaactt cgtcaaaagg
      241 taatggtgtt tcaagcagtg aggcttgttg ttcaacttca ggatgtttac ttgctaactt
      301 ccctatgatg cctttgattg catcagaagt aagattgctt aatgcgccag tgagaacggc
      361 aagtagtgtg gtttcaatca ttatatttcc ctcgttagat tgctgtgatg cgtattattt
      421 actctttata atctccgatt ctgtccatat ggttagttgc actattacgt ttataagtgc
      481 cacttttacc atatatatat ccaacgctac attgtggaca ttttggcgtc tcgtcattat
      541 gagtgaaatt tatttctcct ccgacagata gggtttttac gttttccgga taatctaatg
      601 tagccacgcc ggtccattca caattcgagc agacaacatc gaaagatgtt gggatactct
      661 tgcagttgat gttgatttgc atatgcataa cctcgatttt gcacagaaat tacagtgtta
      721 ttagttgcat accgactata acctgagttt gtgatggagt ggtgtttttt tgctttgact
      781 tgagtaagag aaagagatga tcttcttgag ataatctctt tctgagcgta gtcttttgct
      841 ctgtaaacga aaaaaccacc ctggcaggtg gtttttcgaa ggttagttaa tcctggcaga
      901 tttactaacc gtggtaacag tcttgtgcga gacatgtcac caaagctgtc cttccagtgt
      961 agccgcagta aggccaccac ttcaagaact ctgaatacat ctctcgcaca tcctgtttac
     1021 cagtggctac tgccaatggc gatttgtcgt gccttttcgg gttggactca agacgatagt
     1081 taccgaaaag agcgtggtag tcggactgaa cggggggatc gtgcatacag tccagcttgg
     1141 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
     1201 agcggaatga caccggtaaa ccgaatggca ggaacaggag agcgcacgag ggagctacca
     1261 gggggaaacg cctgggatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     1321 cagattttat gatactcgtc aggggggcgg agcctatgga aaaacggcaa cgccgcatcc
     1381 tcttcccggc ttctcctgat ttgagcgctg cttgcgtatc ccgtcatctg tgagctggta
     1441 acgcccgccg cagtctcacg gcaggagcgc agcgactgag tgagcgagga ggcgtcattt
     1501 cacctttacc agcatgttgc acttacgccc gtcagccaca ttttcccctt cccacatgaa
     1561 gcactttcat acattaccac tccgccgtaa catatttagc cagtatacac tccgctagcg
     1621 ctacgtgact ggttcagggc tgcgccccga aacccgctaa aagccactga cgcgcctgcg
     1681 gcttgtccaa caccgtgccg accgggaaac atttcccgcc cgtcccggtg ttatcaggag
     1741 gctactctgg cagacaaacg caacaagatg ctcacgatgt gggtgacgga agacgaacac
     1801 cggcggctgc tcgagcggtg cgacggcagg cagctggcgg cgtggatgcg tcagtcctgc
     1861 ctcgatgaga agccggcgcg tgcctgcaga ctgccgtcaa tatcgccggc gctgctgcgc
     1921 cagcttgccg gtatgggtaa caacctgaac cagatcgccc ggaaagtgaa tgccgttggt
     1981 gccggtcatg atcgggtgca gattgtggcc gccctgatgg caatagacgc gggactggag
     2041 cggctgcggc atgcggtgct ggaaaaaggg ggggatgatg atcgttaaat ttcatccccg
     2101 ggggcgcggt ggtggtgccg gtccggtgga ttacctgctg ggaaaagacc ggcagcgcga
     2161 gggggcgacc gtgctgcagg gaaaaccgga ggaagtccgg gagcttatcg atgcctcgcc
     2221 gtatgcgaaa aagtacacct ccggcgtgct gtcgttcgcg gaggctgaac tgctgcccgg
     2281 gcagcgggag cagatcatgg ccagctttga gcgggttctg atgcccgggc tcgataaaga
     2341 ccagtacagc attctgtggg tggagcacac cgacaagggg cggctggagc tgaatttcct
     2401 gatccccaac actgaactgc tgaccgggaa acgcctgcag ccgtattacg atcgcgccga
     2461 ccggccgcgc atcgatgcct ggcagaccgt ggtgaacggc agactgggac tgcatgatcc
     2521 aaacgccccg gagaaccggc ggttactggt tacgccgtcc gcactgccgg aaacgaagct
     2581 ggaagccgct caggcgatta cacgtgggtt gctggcctta gcctcatccg gggagctcaa
     2641 aactcgcgag gacgtcacag gtgcgctcac agcggcaggt tttgaggtgg tccgcactac
     2701 aaaaaacagt atcagcattg ccgacccgga cggggggcga aacatccgac tgaagggagc
     2761 catttatgag cagtctttta gcgctggcga aggacttgga gcagcaatcg aaagcgcagc
     2821 agcagagtac cggcgagatg ctgaaagccg cattcagcga gcacgagaaa tctgtaaaag
     2881 cggaactgag cgcaagcgcg aagagaatca gcgacgccat cagcgcccac gagcagggta
     2941 tgaccgcggc gatgcagtcg aaccgcctga gcgtgctgcg tatggtcggc cggacgtggc
     3001 tgaccatcac cctggtctcg gtactgctga tcgccatgag cgggagcatt ctgtggtggc
     3061 aggggcagca gatcaccggg aattaccaga ctatccgggc gcaggagcgg acgcaggcca
     3121 tgttgtcgga gaaaaaccac ggcgttcagc tgtcgctctg cggggagaag aaactgtcct
     3181 gcgtcaaagt gaacccgaaa gcgggagctt acggggagga agggaactgg atggtactgg
     3241 agaggaaata gcccatgacg gaactggaaa aacagttgct gaccgcatta gagcagctac
     3301 atcaggacta ctcgcaaagg ctggacgagt gggagagcgc cttcgcggaa tggcagaaga
     3361 tgtctggtct tatgcaacgg gagaacgcgg cgctgaacga gcgcgtcacg cgcttgagtc
     3421 agcaggtggc gaacttgagc aggcagttac agcgtttgag ccagtagtgc agcggcatga
     3481 aatggcggtg gccgccgaac gggcatacga gcagcgacag cacgaaaaag agctggcgga
     3541 agcccgttcc cgtcagcgga gctataacgg tccgacgctg ggctgacagg cttcaccagt
     3601 gacaaacccc acgaagaagg gctcataaat ttcccgctaa atacggtcgt gatgaaaaca
     3661 tcacggcgta tacaacggag ggattatgag actgtttaac gtgattgaga cggtgctgaa
     3721 ctgcctgtac atggcgctgg agatctggga ctggctggag aagcattttc ctgtttttaa
     3781 ggcgttcatc cagattctgg cctgaaatca gaccgtatgc atctggctgc atgaccatgc
     3841 agccgggtaa aatttatctg tcacgaacgg ggcggtgtat cgggtggttt gttgccggtt
     3901 ttacctgact gccgccccgt ttgtgacgaa catgtccgga gcggtgcggg caagagatgt
     3961 tatggtaaat catattagtt ctgaatgatt cgggctccac cagtcattac tatttgagca
     4021 ccacctgaca tttttatact tgtaccctgg ct
//
