LOCUS       NZ_CP082908             2263 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Mixta hanseatica isolate X22927 plasmid pX22927_4, complete
            sequence.
ACCESSION   NZ_CP082908
VERSION     NZ_CP082908.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20983042
            Assembly: GCF_023517775.1
KEYWORDS    RefSeq.
SOURCE      Mixta hanseatica
  ORGANISM  Mixta hanseatica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Erwiniaceae; Mixta.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Both,A., Huang,J., Wenzel,P., Aepfelbacher,M., Rohde,H.,
            Christner,M. and Hentschke,M.
  TITLE     First case of bloodstream infection caused by Mixta hanseatica sp.
            nov., a novel species within the Mixta genus of the Erwiniaceae
            family
  JOURNAL   New Microbes New Infect 53, 101117 (2023)
   PUBMED   37090948
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Both,A., Huang,J., Wenzel,P., Aepfelbacher,M., Rohde,H.,
            Christner,M. and Hentschke,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.0x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023517775.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 04:35:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,127
            Genes (coding)                    :: 4,045
            CDSs (with protein)               :: 4,045
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 24 of 82
            Pseudo Genes (incomplete)         :: 53 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082908.1.
            Bacteria and source DNA available from Labor Dr. Fenner und
            Kollegen, Bergstrasse 14, Hamburg, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Mixta hanseatica"
                     /mol_type="genomic DNA"
                     /isolate="X22927"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /type_material="type strain of Mixta hanseatica"
                     /db_xref="taxon:2872648"
                     /plasmid="pX22927_4"
                     /geo_loc_name="Germany"
                     /lat_lon="53.551086 N 9.993682 E"
                     /collection_date="2020"
                     /collected_by="Moritz Hentschke"
     gene            526..1521
                     /locus_tag="K6958_RS21110"
                     /old_locus_tag="K6958_21100"
     CDS             526..1521
                     /locus_tag="K6958_RS21110"
                     /old_locus_tag="K6958_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_069067450.1"
                     /translation="MQNPKLQNLTDYSPDDKPWDAHKSQSDDVGGIYLRAAEFESYAAR
                     MRDCGGLLRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDGRWMFLTLTVRNCAIEDLGETLTAMNAAFQRMKVRKEFKPVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPGMLNGHDYVKHYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPDWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDPD"
                     /name="protein rep"
     gene            1760..1945
                     /locus_tag="K6958_RS21115"
                     /old_locus_tag="K6958_21105"
     CDS             1760..1945
                     /locus_tag="K6958_RS21115"
                     /old_locus_tag="K6958_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_000166333.1"
                     /translation="MTKQQRIDGFRKAEASLKLEGMDPSGTPFYESVKARIISGEITYE
                     QGKAEILSHYKARAEG"
                     /name="antitoxin VbhA family protein"
     Typing          complement(1242..1432)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.938"
                     /coverage="98.0"
                     /evalue="1.41e-49"
ORIGIN
        1 cgttcggaag cggggtccac tctgtttaat agtggactct tgttgcaatt tgcaacggaa
       61 acagccgcgt gcagtgaatg taatactgca cgtttgtctc attctgatgc tcagttgcga
      121 gtgtttgagt gctttcatgt ttgagtgttt gaattcactt tttggggttg gtttttcggc
      181 cctgaaaggg tcggaatcct tgtatttata cttaagggat aactctagga tttaaattcg
      241 cttccagccc ttgtgccgca aggcctgaga gccaatttgc tcgaaaatgg ttttgtgagt
      301 atgatttctg acaggtgatc gtgtctgaga actaattttt gattacttgg ggtggttccc
      361 aagaccttac ttactgacac gatgatatga gaaacccctg caagtgctac caacacttca
      421 ggggcgcgat cacctggcta aatacggttt gaaatcgact ctcaacaggt gatacggaaa
      481 tgataacaca cccttccagc gcctgcccat attggagcag gtgctatgca aaaccctaag
      541 ctacagaacc tcaccgacta cagccccgac gataagccgt gggatgctca taagtcacaa
      601 tctgatgatg ttggcggtat ctatctgcgt gctgctgaat ttgagtctta tgcagcccgc
      661 atgcgcgatt gtggcggcct cctgcgtttt ggctggtcta cccttaaaga caccggagaa
      721 acgcgtttac ggctccgtga ggcgcatttc tgccgcgttc gtcactgccc tgtctgccag
      781 tggaggcgct ccctgatgtg gcaagcccgt ttttatcagt ctctgccgaa aattgtcgct
      841 gattaccctg atgggcgatg gatgtttctg acgctcaccg ttcgtaactg cgccattgaa
      901 gatctcggag aaacacttac cgccatgaat gcggcatttc agcgaatgaa ggtgcggaag
      961 gaatttaaac ccgtacaggg atggattcgc accacagaag ttacccgcgg ccgagatggt
     1021 tctgcgcacc cccactttca caccctgatg atggttcctc ctgggatgct gaacggtcat
     1081 gactacgtta agcactaccg ctgggttgaa ctctggcgtg actgcctgcg cgttgattac
     1141 gatcctaatg ttgatgttcg ggcagtgaaa ccccgtaagc ctaaagatgg ccaaacgctg
     1201 gcatgtgcaa ctgccgaact ggtgcgcggt gccgttgctg aaaccctgaa atattcgacg
     1261 aagccggctg atatggttgc tgacccggac tggttcttag aactgacccg gcagacgcac
     1321 aaacgccgct ttgttgcgac cggtggcgcg cttaaagacg ttctgaaact tgagcaggaa
     1381 acggatcagg acatggtgat cggggatgat atttccgatg gtgaagacga tggttcacgc
     1441 ctcgcctttg gctgggattc gacgccaaga aaatacgtcc gtttgcctga gcgcgataag
     1501 tttaaatcag atcctgatta gggtgtcggg gcctgccctg accaggttgt ttttggatgc
     1561 cgctgcgtgt ggcggtactc caaaaacaca tcttgtccgg tacacagatt tcctttggca
     1621 ttccttcggg tttccctgag atttccactc tgcatgcata cacgtataca cgcataacca
     1681 acggcagaat tcgggcagga tatgtaaagt gggcccactt tacgcctgcc ttattcgcat
     1741 cctgctcaaa ggaaaatcaa tgacaaaaca gcaaaggatc gacgggtttc gtaaggcgga
     1801 agcatcgcta aagctggagg ggatggatcc ttctggaact cccttttatg agtctgtaaa
     1861 agcccgaatt atctccggtg aaatcaccta cgaacagggc aaagctgaga ttctttccca
     1921 ctacaaagcc cgcgcagaag gttgaatcgt caacttctaa atcgggggat ccctttaggg
     1981 ttccgattta gtggttgtac gactgcaccc ctcataccct tgtgatgggt gatggttcac
     2041 gtagtgagcc atcgccctgt aaagccgctt ttaccccctt caaggcattg cctgtggggt
     2101 aggtgcgcaa gccctgccct acaggtgatg ccgtgggggt atcctcgttt tgcttggtgt
     2161 gccttgttcg tttaaatctg ccatgaagag tgtcaggggt catgtcgtgc ggccgcgatg
     2221 aggaacgggg attgcccccg cagctctgcg aggaggcata cgc
//
