LOCUS       NZ_CP082907             8411 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Mixta hanseatica isolate X22927 plasmid pX22927_3, complete
            sequence.
ACCESSION   NZ_CP082907
VERSION     NZ_CP082907.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20983042
            Assembly: GCF_023517775.1
KEYWORDS    RefSeq.
SOURCE      Mixta hanseatica
  ORGANISM  Mixta hanseatica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Erwiniaceae; Mixta.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Both,A., Huang,J., Wenzel,P., Aepfelbacher,M., Rohde,H.,
            Christner,M. and Hentschke,M.
  TITLE     First case of bloodstream infection caused by Mixta hanseatica sp.
            nov., a novel species within the Mixta genus of the Erwiniaceae
            family
  JOURNAL   New Microbes New Infect 53, 101117 (2023)
   PUBMED   37090948
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Both,A., Huang,J., Wenzel,P., Aepfelbacher,M., Rohde,H.,
            Christner,M. and Hentschke,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.0x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023517775.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 04:35:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,240
            CDSs (total)                      :: 4,127
            Genes (coding)                    :: 4,045
            CDSs (with protein)               :: 4,045
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 24 of 82
            Pseudo Genes (incomplete)         :: 53 of 82
            Pseudo Genes (internal stop)      :: 20 of 82
            Pseudo Genes (multiple problems)  :: 14 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082907.1.
            Bacteria and source DNA available from Labor Dr. Fenner und
            Kollegen, Bergstrasse 14, Hamburg, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Mixta hanseatica"
                     /mol_type="genomic DNA"
                     /isolate="X22927"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /type_material="type strain of Mixta hanseatica"
                     /db_xref="taxon:2872648"
                     /plasmid="pX22927_3"
                     /geo_loc_name="Germany"
                     /lat_lon="53.551086 N 9.993682 E"
                     /collection_date="2020"
                     /collected_by="Moritz Hentschke"
     gene            404..520
                     /locus_tag="K6958_RS21285"
                     /old_locus_tag="K6958_21045"
     CDS             404..520
                     /locus_tag="K6958_RS21285"
                     /old_locus_tag="K6958_21045"
                     /inference="COORDINATES: protein motif:HMM:NF015750.4"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_350355820.1"
                     /translation="MAPISVHFHTSAGTERVVRNVKSTAGHQRYHPMSKLIF"
                     /name="IS1 family transposase"
     gene            602..1720
                     /locus_tag="K6958_RS21060"
                     /old_locus_tag="K6958_21050"
     CDS             602..1720
                     /locus_tag="K6958_RS21060"
                     /old_locus_tag="K6958_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247497.1"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA];
                     GO:0016491 - oxidoreductase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="WP_249894839.1"
                     /translation="MSSLFSPLNLGGFKLRNRIALPPLTRCRSNQPGNIPGDLMVEYYR
                     QRASAGFMITEGTQIEPRGQGYAWTPGIHSEAQIAGWKKVTDAVHQENGTIFCQLWHVG
                     RVSHNALQPGMAPPVAPSAITATGVRVFIETGPGEGILTAPSEPRELSTQEVKDIVELY
                     RVAAENAKKAGFDGVELHSANGYLINQFISEHTNFRKDEYGGSLDNRLRFLREITEAVS
                     SVFGSHRVGVRFAPLFSSTDEDRVYLGLVEKDPHDTYIRAAKILNDLGVGYLSIAEADW
                     DNSPEMPESFRVALRKTFQSPIMYSGLYTQEKAERILEKGLGDLFGFGRSFIANPDLPE
                     RFKAGYPLNSTDRSSLYGGTEKGYTDYPVYYP"
                     /name="alkene reductase"
     gene            1735..2049
                     /locus_tag="K6958_RS21065"
                     /old_locus_tag="K6958_21055"
     CDS             1735..2049
                     /locus_tag="K6958_RS21065"
                     /old_locus_tag="K6958_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247494.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_094112305.1"
                     /translation="MSPENAIKLLGNPTRVSILKWLKNPEEEFADYSQLFPFEKYGVCA
                     SLIQDKAGLSQPATSLCLKSLHDAGLLEAIKVGKWTYYRRSEFRIKKMTDAVIISLQQL
                     "
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(2172..2612)
                     /locus_tag="K6958_RS21070"
                     /old_locus_tag="K6958_21060"
     CDS             complement(2172..2612)
                     /locus_tag="K6958_RS21070"
                     /old_locus_tag="K6958_21060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249894840.1"
                     /translation="MSGIFLSLIDLQKLSAGARSEVLALIAEGVDEDVFEENGEGPTDL
                     SSLQAEKLVRGLSSKSRSVLKAILENSDSSNGFWCEDLASELEVDVADLTGVWSGLTRR
                     IRTVSGSSDAYLISWAWDDDRQDYYGKMHATTFKNCKKAVNI"
                     /name="hypothetical protein"
     gene            complement(3694..3879)
                     /locus_tag="K6958_RS21075"
                     /old_locus_tag="K6958_21065"
     CDS             complement(3694..3879)
                     /locus_tag="K6958_RS21075"
                     /old_locus_tag="K6958_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009092888.1"
                     /GO_process="GO:0006950 - response to stress [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="WP_249894841.1"
                     /translation="MSVRRGGASNFAENPERAAEVGQRGGRVSGGNFKNNPERAKEAGR
                     KGGQISRRGPSRKSVS"
                     /name="general stress protein"
     gene            4436..4675
                     /locus_tag="K6958_RS21080"
                     /old_locus_tag="K6958_21070"
     CDS             4436..4675
                     /locus_tag="K6958_RS21080"
                     /old_locus_tag="K6958_21070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249894833.1"
                     /translation="MYRNEDEKLSDIIMGEAVLELLDEIAPVTTSSLLLKLQAFLLREE
                     NANKEKAIRHAIDEVKAATERYSSATLVDAAIRH"
                     /name="hypothetical protein"
     gene            5265..5906
                     /locus_tag="K6958_RS21085"
                     /old_locus_tag="K6958_21075"
     CDS             5265..5906
                     /locus_tag="K6958_RS21085"
                     /old_locus_tag="K6958_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008925475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249894834.1"
                     /translation="MDAKTLAVSVADGIASLPMDFYLGIERTFQDLSLSDGGRNIQRRN
                     FGDDERMYRALNKLFRNRAILGQVAEMIIKDALSYLPDPALKKITDKLIGAATSLGSRQ
                     SMQLAITGYLGSKVVGGMMASTITKLSLRLWTGSLVGGIVLQGVLSRASAASRRLRHKN
                     PELWSKLYILDFDMLYFLFEEPLANYIELGETLRKNPLKTEQFLGAIENL"
                     /name="hypothetical protein"
     gene            complement(6131..6364)
                     /locus_tag="K6958_RS21090"
                     /old_locus_tag="K6958_21080"
     CDS             complement(6131..6364)
                     /locus_tag="K6958_RS21090"
                     /old_locus_tag="K6958_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012669271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="WP_249894835.1"
                     /translation="MSVSIKSAKFDPYNMWLQLSDGRVLGVPLTYFPALQNASAEQLAS
                     FELSPRGIHWSELNEDLSLQGLLSLQPLTPIR"
                     /name="DUF2442 domain-containing protein"
     gene            complement(7161..7562)
                     /locus_tag="K6958_RS21095"
                     /old_locus_tag="K6958_21085"
     CDS             complement(7161..7562)
                     /locus_tag="K6958_RS21095"
                     /old_locus_tag="K6958_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_249894836.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKTGELSTCAGVRNSKMIDISE
                     LIRVFGDISSTTVEQPEEQVSGQRVTVSYTANEQLVHSLKQEVEHLRTLVSAQESHIDS
                     LKQSLHLLEHKKETALPVLPAGKWWQFWK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7573..7752)
                     /locus_tag="K6958_RS21100"
                     /old_locus_tag="K6958_21090"
     CDS             complement(7573..7752)
                     /locus_tag="K6958_RS21100"
                     /old_locus_tag="K6958_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_249894837.1"
                     /translation="MTKQQKTALNMAKFIQGQSLLLLEKLNELDLDTEADMCERLHEDA
                     ERLHACLLAKLNQE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7883..8095)
                     /locus_tag="K6958_RS21105"
                     /old_locus_tag="K6958_21095"
     CDS             complement(7883..8095)
                     /locus_tag="K6958_RS21105"
                     /old_locus_tag="K6958_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249894838.1"
                     /translation="MAGRLHVVSKTEKQFLDDAVFAAERAKGKKLSGPEKKEVLRVARE
                     QLLGQREAKAAKQARAEERQAAEFT"
                     /name="hypothetical protein"
ORIGIN
        1 acgaactccc cgtttagtcc gactaccacg cctgttcagg taactgttca cttgattcca
       61 accctgacag acacgacaaa gcgccactgg cggtagccac tggtaacggt ttacggagcg
      121 aagtttgtat agcggactat acacagagtt cttgaagtgt gagcccaact acagctacac
      181 tgaaaggaca gttttggtta ctgcgctccg tttaagccag ttaccttcgg aaggaagagt
      241 tggtagctct aaattccggc aaacgaacca ccgttggtag cggtggtttt tttgtttaca
      301 ggatatgaga ttacgcgcag aggaaaatta tctcaagaag atcattttgg tagaactacc
      361 aaattattga tttagtgtgt agtggagttt ttgaggtgca tctatggctc ccatctccgt
      421 acactttcac acaagtgctg ggactgaaag agttgtccgt aacgttaaaa gtactgccgg
      481 tcatcagcgt taccacccca tgtcaaaact gattttttag ataaagccgc aacaaaatac
      541 atttacatat ttaaatgcat aaatataata tccctttctc tcatctttta ggatattcgt
      601 tatgagttcg ctttttagtc cgttaaatct tggcggtttc aaactaagaa accgtattgc
      661 tctaccgccg ctgactcgct gccgcagcaa tcagccaggc aacattcctg gtgatctgat
      721 ggttgaatac tataggcaac gtgccagtgc gggttttatg atcactgaag gtacacagat
      781 tgagcctcga ggtcagggat atgcctggac accaggaatc catagcgaag cacagatcgc
      841 aggttggaaa aaagtgactg atgccgtcca tcaggaaaat gggacaattt tttgccaact
      901 ctggcacgtc ggccgtgtat cacataatgc ccttcaacca ggaatggctc ccccggttgc
      961 accttcggca attaccgcaa ccggggtaag agtatttatt gaaacgggtc ctggagaagg
     1021 cattctgaca gcacccagcg aaccacggga gctctcaacc caagaagtga aagatattgt
     1081 agagctttat cgtgtcgcag cggagaatgc gaaaaaggct ggttttgacg gtgtagaact
     1141 acattcagcc aacggatatc tgataaacca gtttatttct gaacatacta acttccgtaa
     1201 agatgaatat ggtggctcac tcgataaccg actgcgtttc ctacgcgaaa taaccgaagc
     1261 tgttagttca gtatttggta gccatcgagt tggagtacgc tttgccccct tattttcgag
     1321 cacagatgaa gatagagttt atcttggtct tgttgaaaaa gaccctcatg atacttatat
     1381 acgtgcagca aaaattctga acgatctcgg agtaggttat ctttcaatcg ccgaagctga
     1441 ctgggataat agcccagaga tgcctgaaag tttccgtgtt gccctacgta aaacgttcca
     1501 atctccgatt atgtattctg gtctttatac tcaggaaaaa gctgaacgta ttttagaaaa
     1561 aggcctgggt gatttgttcg gttttggacg ttcatttatt gctaacccag acctccctga
     1621 gaggttcaaa gccggttatc cattaaatag tactgacaga tcctctctat atggtggtac
     1681 tgagaaagga tatactgatt atcctgttta ttatccctaa ttttgtgagg cagcatgtca
     1741 ccggaaaatg ccattaagtt actaggtaac cctactcggg tttctatcct aaaatggctg
     1801 aaaaatccag aagaggaatt tgctgattat tctcagcttt ttcctttcga aaaatatggt
     1861 gtctgtgcca gtctcattca ggacaaggcg ggtttatcac aacccgcaac ctcgctatgc
     1921 ctaaaaagtt tgcatgatgc tggactactt gaagcgataa aggtgggtaa atggacttat
     1981 taccgccgca gcgaatttcg cataaaaaag atgacagatg ctgtcattat atctctacag
     2041 cagttataaa acggaaagaa gatgctacaa atattcatat ttaccataac atccctgttg
     2101 tgatctgacc tgttatgggt gcaccgccac acagaacatc taaaaaaaat cccgctatcg
     2161 cgggcttttt tttagatgtt tacggctttt ttgcagtttt taaaggtagt tgcatgcatt
     2221 ttgccatagt aatcctgtcg gtcatcgtcc catgcccatg aaatgagata agcatctgat
     2281 gagccactaa cagtcctgat tcgacgagtc agacctgacc atactcctgt cagatcagca
     2341 acatccactt cgagttcaga tgcgagatct tcacaccaga aaccgtttga gctgtcactg
     2401 ttttcaagga tagccttcag tacagaacgt gatttactgc tcaggcctcg aacaagcttt
     2461 tccgcttgga gcgatgataa atcggtaggg ccttcaccgt tttcttcaaa aacatcctca
     2521 tccacgccct cagcaataag ggctagaact tcgctgcgtg cgccagctga aagcttttgc
     2581 aaatcgatca atgacaaaaa aattccactc atgtctgact tctcctcgtt gatttgaaga
     2641 aagactaaca cagactaagc gtttgacgat acttaaatgc attaaacgat aaggcaaaca
     2701 tctactaagc aaggcttatc tcaaattatg catttttgct ttgataattt ctcttaaggt
     2761 gaacataaaa cccatccctg atttgaggtt gatttaccat aacatctctg tagcgcatca
     2821 aaccgaaccc tgacatatca gaacacgccg ttcttttaaa aggattttta tggtaatact
     2881 ggataaccat ccattttact gacccgaagc tgtgatgaca cctgacaaac ccgacaggga
     2941 gctccctctc cttgctacag aagaatggct tacaccgaac cacagtacgc agcaggataa
     3001 aaacagcgac tggatttcat ctctggagag agaactggag gaaatctcac aaaaatggcc
     3061 cttcccgcgt agttttacat gacgttgctt tttcacatgc tcgcgtaacc caggctcaga
     3121 ctgtggcagc cgcataaaaa agcccgtatg catgaggctt ccaccacaac ttcgtcagtt
     3181 ggcgtcaaaa aaacccttcc tttgcagcgt taaacgttgc actaccggca gcgcctatta
     3241 taactacagt tcattaacac gcattaattt aaaaggacag gataatgtat cagctatttt
     3301 ttcgctgccc tgaatgcggg aacctaaagt ttatatacac tacccggaac gaaaagcggg
     3361 agactcctca cggtgctgta tgcagccggt gcggcacgcg ccttacggtt gcctcctgtc
     3421 tgttatccgg aaagccccct cctccaagcc agacccgtga ctgactaacg gctcgcaggt
     3481 ttatacgggc gtgctataaa aggtggtaca tcagctgtta tggctggaga tccgggttca
     3541 actccagtag cccgttccat ttccttctac gtcacgtcaa aagcgtttcc caggtacagg
     3601 tctcacctca ggaataacca gcttcattta aaataaaagt gcttttgcgc aggccattaa
     3661 aaagcccgca atgcgggctt tcttcatagc ggttcaggat accgattttc tagaaggccc
     3721 tctccggctg atttggcctc cttttctccc tgcttccttt gcacgctccg ggttgttctt
     3781 gaagttacct ccgcttacgc gaccgcctcg ctgtccaact tctgcagccc gctccggatt
     3841 ttcggcaaaa ttgctggcac caccacgacg tactgacata aaacccccag tttgagttaa
     3901 gttccatcat cccaaagcat aggagacatt attctaccgg taggttttta acagggactt
     3961 gtgaagctgg caaaaaaaac ggcgccgtag cgccgtgaga atcagttttt agtgtgcaga
     4021 atccccgggt gagggctaca tgtttatcgg ctttgcacag tgaacattta ttaaaatacc
     4081 tcctccccgg atacaaaaaa accccgccat cttgcgcggg gtgaataaag aagaatccaa
     4141 acgtactctc ggtaaacgga accagcagga accgtcacct cttaagcctg gtaaagaacg
     4201 atccgttact caagcgaagc caggcacccg cagccggcgt tgtcgtaccg gatcccccca
     4261 ggcatattta acataacagt actgttaagc atgcggctgt aggctaagcg cttatcttaa
     4321 agatgcatct gataaatacc ttttgtgctc cttccctgcc ggagcgacag ttttaacgta
     4381 tcctgtaggc ttcgcctgcc gtacgggtag agctttcaca gcaagaaggt cacgcatgta
     4441 cagaaacgaa gacgaaaagc tgtctgacat aataatgggc gaagccgtcc tcgagctgct
     4501 agatgaaatt gcgcctgtca caaccagctc gctgctactc aagttgcagg ctttcctgct
     4561 cagggaagaa aacgcaaaca aagaaaaagc tatccgtcac gccattgacg aggtgaaagc
     4621 ggcaactgag cggtacagca gcgccaccct ggtagatgcg gcgataaggc actgatgcag
     4681 tttcctgatc acctttgcca gccgctcgga tttaccataa catcccttgg gcgcacccct
     4741 gcagagcccc tcagcgcgat tctgccgcgt ccgggccaga atccagccct ttccctgccc
     4801 ggaaccgcga aaatccgccc tgataatttc tgaccggctg catggctatg cacgcaggtg
     4861 aatagcgggc ggtttcacgc gtatgttgtc actaagcccc gttttccggc gcttaactcc
     4921 tctgataacg gtgaacgggc gcgcagttta ctgtgcggcc gtgaggcgtt ggccaagccg
     4981 ccaggcgcgg cagaaaacag atggtgccat tcgctaccac tttgcgaatc ctgccctccg
     5041 tctgaatcgc agaaaaattt tgtacgtacg aaattcaggg tattgggctg tgcctgagcg
     5101 gctggcttct ggtacgccag ctccttacgc tggcagggga ctgaatgaca aaatcagatc
     5161 cgtttaaaac gataatccgg acgctttaaa gataagtttt cgtttacgaa aagtggctct
     5221 gcctgatatt tttcagactg gtataacaga cggtaagaga attaatggac gcaaaaacac
     5281 tcgccgtatc ggttgctgat ggtattgctt cacttccgat ggatttttac ctgggtattg
     5341 aacgaacctt tcaggatctc agcctgtcag atggcggcag aaacattcag cgtcgtaact
     5401 tcggtgatga tgagaggatg taccgcgctt taaataagct cttccgtaac agagccattc
     5461 tggggcaggt cgcggagatg ataatcaaag acgctttatc atatcttccg gatcctgccc
     5521 tgaaaaaaat cacagataaa ctcatcggag cagcgacttc actgggttca agacagtcaa
     5581 tgcagctggc cattacaggt tatcttggct caaaagttgt tggcggaatg atggccagca
     5641 caataacgaa gttatcactg agattatgga caggctccct tgtgggcggc atcgttttac
     5701 agggggtgct ttcgcgtgct tctgcagctt cccggcgttt aaggcataaa aaccccgaat
     5761 tatggtcaaa gctttatatc cttgattttg atatgcttta cttcctcttt gaagaacctt
     5821 tagctaatta catcgagctt ggtgagactc ttcgtaaaaa ccctttgaaa acggagcaat
     5881 ttctgggtgc aatcgagaat ctttagacta atcagaaaag tcgtttctga gttatccggg
     5941 gcggtggtta tcagcgccgt agttatagga attttcatcg caatttttgc gaatgaaggg
     6001 attatgcgcg ttatcgctcc cgttctcgtc tttatcgcag gcctggttct ttactggtta
     6061 gcctggatga tttcatcaaa agaagatcgg aaataggcaa ctcaaaaaag cattactgtg
     6121 ggcaacattg tcaccgtatg ggcgtcaaag gctgcagtga cagtaatccc tgtaaagaca
     6181 ggtcctcatt cagttcactc cagtgaatgc cgcgcggact cagctcaaaa cttgccagct
     6241 gttcagcgct ggcattctgc agcgcaggaa agtacgtcag aggcacgccc agcacgcgac
     6301 catcgctcag ctgcagccac atgttgtagg gatcaaattt tgccgatttg atggatacgc
     6361 tcatcacgac ctccggttga ccgtcacgaa tttacgttga tagtatgccc gggtagtcct
     6421 gtcgtccatg acgaccacca atgatcccac ggcctgagag gatcgccttc gggtggctgt
     6481 acagaacact cccgcagcct gaaagggaga acgcaaagcg tggtacatct gcccgcccct
     6541 ggaagcagat atcgtcacaa acagcgcacc tacgacgcga cgatgagggt acatttcaca
     6601 atgcagtgac cggcaccgac cgtcctgtca tccgctgccg gcagtcataa ccacgacagc
     6661 ccgctacacc ctgcgatcac cattacgtct tccggaaagc acctgtttgc agaggcaaac
     6721 gcgcctccgg actgaacggc gtcactttgg ttcgagtgaa acgagaacaa agtgacgcgt
     6781 gaaaaaacca cgctgcagcc cctgcggtac aacgcctgac agcgatttcc acaatctgac
     6841 gtgcacgtat tacgtgtttt catgcgccct gaacgggctt ttaaccacaa cgctgccggc
     6901 gcgaccgcag gcaggtttca ttaagaaagg aaaaagaacg gctcggtatc gatggcgaca
     6961 ccaggagact ggtgtgtgga gggagtctga cgcaagtcag cccggcgtaa gccgcgaaca
     7021 ggtcagcgac ctggggaaac ctcgcaagaa ctcaacaatt cgtcaccccg atgcacagat
     7081 accagccggg acgcaggaag cgcaaaagca accgcgccgt cggggccaga attaccggaa
     7141 aagcaggtgc tgtcagccgg ctatttccag aactgccacc atttgccagc aggcaaaacg
     7201 ggcagtgctg tctctttttt atgttcgagg agatgcaggg actgcttaag gctgtcgata
     7261 tgcgactcct gagcagaaac cagtgtacgg aggtgttcaa cttcctgttt caggctatga
     7321 acgagttgtt cattagctgt ataagacact gtaacacgct gtccagatac ttgttcttct
     7381 ggctgttcaa ccgtagttga agagatatcg ccgaaaacac gaataagctc tgatatgtct
     7441 atcatttttg agttgcgtac acctgcgcag gtagacagtt cacctgtttt tataagcctg
     7501 tgcagcgttg tcctgctttt acctgtaagc ctggcggcct cactgataga tactttcgcc
     7561 atgcggatac cgttattcct gatttaactt tgccagaagg caggcatgaa ggcgctcggc
     7621 atcctcatga agccgttcgc acatgtccgc ttccgtatcc aggtccagct cgttgagctt
     7681 ctccagcaga agcaggcttt gcccctgaat gaattttgcc atgttcagcg cagttttttg
     7741 ctgtttcgtc atcagacgct ccgttgcgta cgtaccatat gctgaaaaca ggtgaaatga
     7801 cgcttcctcg gttcccctgg ctgcgccaga cgaacactgc ggcgagcgtt accggctaac
     7861 gacgaaaagg cttaggcttg ctctaggtaa actcagcagc ctgacgttct tcagcccgtg
     7921 cctgtttagc ggctttcgct tcacgttgtc ccagcagctg ctcacgggca acacgcaaca
     7981 cctctttttt ttccggcccg gacagctttt tgccttttgc ccgttccgct gcaaagacag
     8041 catcatcaag gaactgcttt tcagtcttag atacaacatg caggcgacca gccataaatc
     8101 ctccaggtca atgtcaacag cgcccccctt tccccccgag cctggtcccc ggaaccccgg
     8161 ttttccatag gctccgccct cctgacgagc atcacagaaa tctgccgatt cccggtgctc
     8221 aaatcagaag gggcgaaacc cgacaggact taaagatacc ggacgtttcc atccggtggc
     8281 tccctcgtgc gctctcctgt tcccacctta cagcttagcc cgtgtctttc cgctgttatg
     8341 gccgcgtttt ctcataccac gcctgacact gggtgctgct taggtggttc gctccaagct
     8401 ggactatatg c
//
