LOCUS       NZ_CP082795             4572 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP4823 plasmid pKP4823_5, complete
            sequence.
ACCESSION   NZ_CP082795
VERSION     NZ_CP082795.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18789105
            Assembly: GCF_018138305.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ruan,Z.
  TITLE     Genome sequence of a Klebsiella pneumoniae isolate carrying mcr-1
            gene in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2021) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
REFERENCE   3  (bases 1 to 4572)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2021) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:01:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,342
            CDSs (total)                      :: 5,220
            Genes (coding)                    :: 5,118
            CDSs (with protein)               :: 5,118
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 59 of 102
            Pseudo Genes (incomplete)         :: 44 of 102
            Pseudo Genes (internal stop)      :: 24 of 102
            Pseudo Genes (multiple problems)  :: 20 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082795.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP4823"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP4823_5"
                     /geo_loc_name="China: Yiwu"
                     /collection_date="2019-02-28"
                     /collected_by="Jianyong Wu"
     gene            96..434
                     /locus_tag="KCV57_RS26565"
                     /old_locus_tag="KCV57_26565"
     CDS             96..434
                     /locus_tag="KCV57_RS26565"
                     /old_locus_tag="KCV57_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_155559896.1"
                     /translation="MAKVTRIQGGQYAVDDGRFIVKNSSGWLVVDANGSHDFGPVPTLT
                     AAKQYVESGTTPLGQHNFSTNYGRRQSKKEFNAYLASEAKNGNPLPAILWFIVLCIICG
                     VVLAVRGY"
                     /name="hypothetical protein"
     gene            complement(595..858)
                     /locus_tag="KCV57_RS26570"
                     /old_locus_tag="KCV57_26570"
     CDS             complement(595..858)
                     /locus_tag="KCV57_RS26570"
                     /old_locus_tag="KCV57_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_211588474.1"
                     /translation="MAVYTSETKQFLDSVSHDKTWWTSRYNPADDVPVSGIYRCTVCGK
                     EITSNKDDPFPSQNHHQHSQSQAIKWQLIVRTDTQGNGCGSK"
                     /name="protein L"
     gene            complement(1022..1438)
                     /locus_tag="KCV57_RS26575"
                     /old_locus_tag="KCV57_26575"
     CDS             complement(1022..1438)
                     /locus_tag="KCV57_RS26575"
                     /old_locus_tag="KCV57_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_057103209.1"
                     /translation="MSDQKNNLDLQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            1786..2460
                     /locus_tag="KCV57_RS26580"
                     /old_locus_tag="KCV57_26580"
     CDS             1786..2460
                     /locus_tag="KCV57_RS26580"
                     /old_locus_tag="KCV57_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_096908159.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRARESQL
                     IQVKFFTAEIKAKFASQGELAHQAQQAYHRALVQIYGDKLQIIKGYYSERQECAMTFNE
                     GQPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEAEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISSLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            3332..3523
                     /locus_tag="KCV57_RS26585"
                     /old_locus_tag="KCV57_26585"
     CDS             3332..3523
                     /locus_tag="KCV57_RS26585"
                     /old_locus_tag="KCV57_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127900087.1"
                     /translation="MKPSNNLSAIGYHPMPSEYDEQLGSTDTQLPCEYDLFDLMAQVTE
                     ENLHAPVDFGPPVGREAL"
                     /name="hypothetical protein"
     gene            3906..4274
                     /locus_tag="KCV57_RS26590"
                     /old_locus_tag="KCV57_26590"
     CDS             3906..4274
                     /locus_tag="KCV57_RS26590"
                     /old_locus_tag="KCV57_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001273382.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPENGFFLSENDFMRAEEE
                     SQRMVESFRDSLGRKKGF"
                     /name="hypothetical protein"
     Typing          3167..3381
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.532"
                     /coverage="100.0"
                     /evalue="7.33e-68"
     Typing          complement(2703..3191)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.129"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 acgctctcag agcctctcag gcggtgcacg caacagatgt tatggtaaat acaatgagct
       61 gctgaaagcg gcctgataga cattaggaga atgtattggc gaaggtcact agaatccagg
      121 gtggacaata tgcagtggat gatgggcgtt ttattgtaaa aaatagctca ggctggcttg
      181 tggttgatgc caatggttct catgattttg gacctgttcc tacgctaacc gctgcgaagc
      241 aatatgttga gtctggaacc actccgttag gacagcataa tttttccacc aattacggtc
      301 gcagacagag caaaaaagag tttaatgcat atcttgcctc tgaagctaaa aatggaaacc
      361 cgctgccagc catcctttgg ttcattgttc tttgcataat ctgtggcgtt gtattggcag
      421 ttcgaggtta ttaggtaagg ttgatatgca tatcctttgg tctctatgga ctatcgacgt
      481 gaagcctacg actgaagccc gcaaagcggg ctttttcatg cccggcggac aacggatgtt
      541 atggtaaatc cagctgattg gcaggtacaa aaaaagcccc taagggcttt tgagttattt
      601 agaaccacaa ccatttcctt gcgtatctgt cctgacaatt aattgccatt tgattgcctg
      661 actttggctg tgttgatgat gattttgcga cggaaacggg tcatctttat tgctggttat
      721 ctcttttccg caaacagtac aacgatagat acctgataca ggtacatcgt cagcagggtt
      781 atatcgtgat gtccaccaag ttttatcgtg tgaaacactg tctaaaaatt gtttagtctc
      841 agaggtgtat acagccattt accttgcctt tccaaaatgt atagggtggg ggtatcctct
      901 ttataagtat ggttttttta gagtattttc aatcatcggg tgatgattga aaatactcta
      961 aaatagagat gcgatggtaa atctgaattg taatggtttg atttttaaaa ttattccatg
     1021 gttacttttc gttcagcttg gcattgaggt ctggcgttag aatgtttagt gacttaacta
     1081 atagagacat atcgtaaatg tttcgccctt tgtacttagg gttatccttg cttgtacgaa
     1141 tttttgtaag caatccgtaa ttttcaagtt cttggagtga gcgttgaact gttcttgatg
     1201 aaactcccat tctaaccgcg atgctctctt gagatggatg tggccaatca tagggtttcc
     1261 accagtgaat aattagatga agtagcacat tgaatgttgt gggggtcata ttgagtgcgc
     1321 cctgcaggaa gaaaagggat gtaggaatgg ctgaccaccc catgcttaat gcttccttcc
     1381 cccatttaga ttcaagtgtc tgtttttgaa ggtctaaatt atttttttga tctgacatat
     1441 accaacctgc taagctgaag tcttctaaga agacttcagc ttagcaggtt ggtaaattaa
     1501 ttcatataat tagtacgaca ccagcgcgct tataatatca ttatatttaa ctatacttaa
     1561 tctacactca cctcttggta gcgcgattaa gttacgcagc tcccttgagg ctctaaacct
     1621 ccctttaggt gggcagcgat taagttacgc gtcttccttc aggagcttga aaccgccctc
     1681 acggttagtt ttaaccctga ggaactacaa cttaaactgt ttgctaacgt tagtttttgt
     1741 tgttaaaacc gcccttagag gcggtttttt tatgcgcgaa ataaaatgag aacaatagtt
     1801 tacgtagatg ggttcaattt ttatcacgga ttggtgaagc gttcgggata taagtggtta
     1861 gacctttata agctctttga agaacaactg cttccgataa gagcaagaga atcacagttg
     1921 attcaggtga aattctttac tgctgagatc aaagcaaagt ttgcttctca gggggaattg
     1981 gcgcaccagg ctcagcaagc ctaccatcga gcattagtac agatctatgg tgataagcta
     2041 cagataatca aaggttatta ctcagagcgt caggaatgtg caatgacctt taatgaaggg
     2101 cagccgccat caaaaacgga tagaacaacg gtctggaagt tagaagaaaa gcagaccgac
     2161 gttagaatgg ctttagaaat gtatcgcgat gcgattcgtg gtgaggcaga acagttagtt
     2221 ctctgcacta acgactcaga tatgataccg ccaatagaaa tgataaaagc cgatcggcct
     2281 gagttaaaaa taggaatcgt agtgccaagg gaacaagata ctagtcgagt gattagtagt
     2341 ctggctagtt taggggattg gagcttgcca tatgtatcct atgaagcctt aaggttagct
     2401 ttattgcctg ataaagtccc cactaggcgg aagccaatac tgaagccaaa tcactggtaa
     2461 acagccggat cttcttgtga tccttttact ctcgtcgtaa tctcatgtcc tgcaaacaaa
     2521 aaaaccaccg ctaccaacgg tggttcgttt gccggaatca agagctacca actctttttt
     2581 ccgaaggtaa ctggctttca cggagcgcag ataccaaaac tgtccttcca gtgtagccgc
     2641 agtcaggcca ccacttcaag aactctgtag aagcactcta ctactctgct ccgtgaatcc
     2701 tgttaccagt ggctgctgcc agtggcgctt tgtcgtgtct ttccgggttg gactcaagat
     2761 gatagttacc ggacaaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca
     2821 gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga taaacgcggc
     2881 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag
     2941 ctaccagggg gaaacgcctg ggatctttat agtcctgtcg ggtttcgccg ccactgattt
     3001 gagcgtcaga ttttgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggcagcgcc
     3061 gtatcctttt tccggccttt cctgacttgc gtgccatctt tttatcttgt cattcgtgag
     3121 caggtaacgc ccgccgcagt ctcacggcag gagcgtagcg accgagtgag cgaggaggcg
     3181 tcatgtcacc gttacctgca ttttgcactt acgtcctata gccgcatttt cccttgcaac
     3241 atgaataact ttcctgaatt accactccga cctaacatat tcagcctgta tacactccgc
     3301 tagcgctacg tgactggttc agggctgcgc cttgaaaccc tctaacaacc tctcagctat
     3361 tggatatcac cccatgccat ctgaatatga tgaacaactg ggttcgactg atacgcagct
     3421 tccctgcgaa tatgaccttt ttgatttaat ggcgcaggtt actgaagaga acctgcacgc
     3481 cccggtggat tttgggccac ctgtcggccg tgaagcgtta tagctttttc gcttaccccc
     3541 gtacgtgtaa tacgccggta ttacatctta tttctttatt ttcatgtgat tacaggtggt
     3601 tgtaatacgt tgcgtattac ataaataaac gggaaattca gtttttacct tttaattcat
     3661 gcaattgcga tgaaaatcgc ggttgcgtat ggtgtatggc catttaaggg ataatgtaac
     3721 ctgactttta aagaatgaat ctatctttgg cagcgcatga gatcgataaa aagcgttcca
     3781 cattaggcgg ttcatggata catgaaccgg aaaggacaaa aataagacaa ttgtaagaca
     3841 ttttttacgt tttactctta tttttggact attatgagac aagtgtaaga cattatgggc
     3901 ggtatatggc gaattttaac cctggtccaa gaacagccat tgcagctgtc agggattcca
     3961 agaacgatat agaaacctgg aagaaaaatg gtatccgtag ggaagagatt gtgactgtgc
     4021 tgaatgaacg ttacggcctg aatatctcac tggttgcgtt tgataaggct ctttacagat
     4081 tgagaaaaag tacagatgaa ccgtctcttg ctgatgtgga ttctgtgtct gctgcagaaa
     4141 cgaatttctc tgaagccagt gggcctgaaa atggtttttt tctgtccgag aatgacttca
     4201 tgcgagcaga agaagaatct caaagaatgg ttgagtcatt cagagatagc cttggcagga
     4261 agaaaggatt ctgattttga attctaagta gtgaaatagt ggatcctcta ctcgaaattg
     4321 tcggacacct gcaattacct accgtgcctt acggcttggt caatgcctta cggccacgca
     4381 ataaattgcg tccccgttca ccgttttcgg ataagaagaa catcggaaac aggacttacc
     4441 ccggctgaat ggtgtgaaat tctgcgctat gcacttgcgc gcatactcat gcatgccgta
     4501 aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt ttgttgcctg ttttaccggt
     4561 ttcccgtcag aa
//
