LOCUS       NZ_CP082769            11970 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 12 plasmid pKP12_5, complete sequence.
ACCESSION   NZ_CP082769
VERSION     NZ_CP082769.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12389483
            Assembly: GCF_007955105.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Ruan,Z.
  TITLE     Whole-genome sequencing facilities tracking of a nosocomial outbreak
            of carbapenem-resistant Klebsiella pneumoniae sequence type 11
            strains carrying capsular loci KL64 and rmpA/rmpA2 genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2021) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Ruan,Z. and Jia,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2019) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Oxford Nanopore; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:47:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,953
            CDSs (total)                      :: 5,831
            Genes (coding)                    :: 5,652
            CDSs (with protein)               :: 5,652
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 66 of 179
            Pseudo Genes (incomplete)         :: 120 of 179
            Pseudo Genes (internal stop)      :: 25 of 179
            Pseudo Genes (multiple problems)  :: 32 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082769.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="12"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP12_5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-12-25"
                     /collected_by="Huiqiong Jia"
     gene            complement(join(11924..11970,1..103))
                     /locus_tag="FTO26_RS29425"
                     /old_locus_tag="FTO26_029425"
     CDS             complement(join(11924..11970,1..103))
                     /locus_tag="FTO26_RS29425"
                     /old_locus_tag="FTO26_029425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(188..445)
                     /locus_tag="FTO26_RS29430"
                     /old_locus_tag="FTO26_029430"
     CDS             complement(188..445)
                     /locus_tag="FTO26_RS29430"
                     /old_locus_tag="FTO26_029430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(455..2140)
                     /locus_tag="FTO26_RS29435"
                     /old_locus_tag="FTO26_029435"
     CDS             complement(455..2140)
                     /locus_tag="FTO26_RS29435"
                     /old_locus_tag="FTO26_029435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(2245..3732)
                     /gene="ltrA"
                     /locus_tag="FTO26_RS29440"
                     /old_locus_tag="FTO26_029440"
     CDS             complement(2245..3732)
                     /gene="ltrA"
                     /locus_tag="FTO26_RS29440"
                     /old_locus_tag="FTO26_029440"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            4377..4622
                     /locus_tag="FTO26_RS29445"
                     /old_locus_tag="FTO26_029445"
     CDS             4377..4622
                     /locus_tag="FTO26_RS29445"
                     /old_locus_tag="FTO26_029445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4896..5267
                     /gene="tolA"
                     /locus_tag="FTO26_RS29450"
                     /old_locus_tag="FTO26_029450"
     CDS             4896..5267
                     /gene="tolA"
                     /locus_tag="FTO26_RS29450"
                     /old_locus_tag="FTO26_029450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            5264..5629
                     /locus_tag="FTO26_RS29850"
     CDS             5264..5629
                     /locus_tag="FTO26_RS29850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            5804..6082
                     /locus_tag="FTO26_RS29460"
                     /old_locus_tag="FTO26_029460"
     CDS             5804..6082
                     /locus_tag="FTO26_RS29460"
                     /old_locus_tag="FTO26_029460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6070..6384
                     /locus_tag="FTO26_RS29465"
                     /old_locus_tag="FTO26_029465"
     CDS             6070..6384
                     /locus_tag="FTO26_RS29465"
                     /old_locus_tag="FTO26_029465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6548..6976
                     /locus_tag="FTO26_RS29470"
                     /old_locus_tag="FTO26_029470"
     CDS             6548..6976
                     /locus_tag="FTO26_RS29470"
                     /old_locus_tag="FTO26_029470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7002..7181
                     /locus_tag="FTO26_RS29475"
                     /old_locus_tag="FTO26_029475"
     CDS             7002..7181
                     /locus_tag="FTO26_RS29475"
                     /old_locus_tag="FTO26_029475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7208..7738)
                     /locus_tag="FTO26_RS29480"
                     /old_locus_tag="FTO26_029480"
     CDS             complement(7208..7738)
                     /locus_tag="FTO26_RS29480"
                     /old_locus_tag="FTO26_029480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7745..8476)
                     /gene="mobC"
                     /locus_tag="FTO26_RS29485"
                     /old_locus_tag="FTO26_029485"
     CDS             complement(7745..8476)
                     /gene="mobC"
                     /locus_tag="FTO26_RS29485"
                     /old_locus_tag="FTO26_029485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(8476..10440)
                     /locus_tag="FTO26_RS29490"
                     /old_locus_tag="FTO26_029490"
     CDS             complement(8476..10440)
                     /locus_tag="FTO26_RS29490"
                     /old_locus_tag="FTO26_029490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            11561..11710
                     /locus_tag="FTO26_RS30070"
     CDS             11561..11710
                     /locus_tag="FTO26_RS30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_308460525.1"
                     /translation="MGCKQELPVQPDCCALSGNFHLESNPGKHAKTPLAAAIGNWEFAE
                     DLFS"
                     /name="replication initiation protein"
     BGC             1..7140
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          11243..11821
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.77"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7749..8476)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ccgttccgcc ctgaacatcc cggatataat ttgcctgaca tgcggccaga agaaagccag
       61 caacaatcaa tataaaaaga aaaatagtct ttaccttttt catatacttt ccctgtattt
      121 aaagactccg gcattcatcc ctgaacaccg gaagtcagta tttcaaatca tccttgataa
      181 ttttttgtta ccagttaccg tcccgataat caaaagctat ataacaatta tccacatcag
      241 gttttatttg atttttaaaa aatggctgta gtaaaggaac ccattctttc ttcaaatcaa
      301 actcaccatt attaatatta tcctttaaag gcattcccaa gctttcaatg acagaaccat
      361 cgtcaccaaa gtctttagaa tactcttccc ctttaaactc ttcagttttc ttatcaaacc
      421 atgtcaaacg taatttaagc cccatattta cctctcaaag atatttttta atgttccgtt
      481 ttggatctgg ccctttaacc tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc
      541 catcactggc acgataccct tcaagctcac cgtgctggga atcccattca taaattttac
      601 gttttttatc gccataccat cgggcccgct taccaccacc accttgttta ggtgttttag
      661 gatcacctcg ctttaactct ccaagtccct ttatgtcttc tgttttaggt actggatgat
      721 aatcatggcc gtagtcttta gtccccttgc gaggcttgtt cttttcctca tttaacttat
      781 tttctgcatc cttcgctttt ttctcctttt gtttccggct ctccatcgcc ccggtaagtg
      841 cagccttatc tctggataag gattcattca ttgcgtttaa agatgcctga gccgctttta
      901 atttctcatt ctgattctga atatccttat caagatagcg aacctgatcc tgccacccgg
      961 agccatattc atgaggcggg ttataagtca caaaatcatc tttatttgcc tctgcctctt
     1021 ttaccttttt atcggcttcc tgaacagcag cccttgcacc ggggagcgcc ttctctgtcg
     1081 atgcaatgcg gctgtttaag gtcgcaatat ttttatcttc ggcatccagc tcagctttcg
     1141 ctttatcata gtctcttttc agcccctctt ccggatgagc ggcgtcccat gcctgctggc
     1201 gacgcttttc ttcctcctga cgctgtttta cctgtgccgg ggtaagaaca tctgtcaccg
     1261 acacataaac cggcttctgt ccggtctctt tcgggaaacg aataacggcc tcacgggagt
     1321 taccaccggc tgtaaaacca gccggacgtg aatcaccttt ttcagcaaca atgccttttg
     1381 gcggggcttt cgctgtcgga acacctttcg gtacgctcac ctgcagagac gggagacccg
     1441 gaatcgacac actgaatacc cccggacgtt ttgtcggttt cgcatccacc acagggacac
     1501 tcattggccg acctgcgaca accgcaatat gctgccgctc atccttcacc acatccacaa
     1561 cccgttgtct gacacgaacc gtcgcctggt ccagtggtaa agtacttgcc ggagtctccg
     1621 tcaccgcatc ggccggtaat gacgtcataa tttttgacat cattttcgga tcatcttttg
     1681 caatctcaga aggcagcaca ccgtagatcg caatccccca cagaccaaac ttaaacggcc
     1741 ccttcagggc agccagcaca tcagcaacgg cggccgacaa cgcatcgccg gaaactgata
     1801 aagccagtcc ttcagccccc ggagtagcca gggcaggcag accgaaggcc atcgcagtgg
     1861 ccgaagactg tccgccacca gagctaccgc tatgattgcc gctgttacca ccattaccac
     1921 cgggtttact cccgccgtta ccaaactccc ccggattata gttatgaata tctccattcg
     1981 gcgtatgcgt cgtaccccag cccggcccgc tgtttggatc cgtaccacca cctgatgaag
     2041 ggccaccttt accagacgta ccgttcacat tcccactggc ctgaccacca ttatgtccca
     2101 gacctgaatt acccggacct cgaccgtctc caccactcat accgttttcc tctttgacat
     2161 ttaaaacaat aaattaaagc caacatgtcg cggtagggat acccgttacc ggataccccc
     2221 cgcacagatc ccggcgtgcg cgatttacgc accgggctcc tgcctcgggt gtctggcggt
     2281 gaaccgctcc acaggccatg gatgaagaac ccgaaccctt ggtagccatg cggctgccag
     2341 tttgtttgct ttcgtccagg tcgtatcatc cttctggctc ctgcgcctga gcgcccggcg
     2401 ccagaggttt gttacgtgtg tcctgaactt ctgcatggtg gggaagttgc ccggtaccga
     2461 gtgatagttc aggtatccct gaaccactct cctgagccat tttccctgtt cggggattga
     2521 gtaatgccag cgccttcgca gaccgtcttt gatggctttc agagttgccg tcatccgatc
     2581 ccggcgggtc tttcgtatca gcatgaacct gccgttgcga tctttcccgc tgatgtgcgt
     2641 gaacccgagg aagttgaacg tttctggttt gccttttccc ctgatggcac ggttttcggc
     2701 agcgaagcgg ccgaactcca tcagacgggt tttctccggg tgaaccgtga gtccgaactc
     2761 cctcagtctg cgctgcatgg ctatacggaa gcgccgggca tcgtatcgtt tgtcgaaccc
     2821 gatgacgatg tcatcggcgt atctgaccat taccacattg cctgtggcat agcgacgtcg
     2881 ccactgatgc gcccacagat cgaagacgta gtggaggtat atgtttgcca gcagcggtga
     2941 gatgaccgca ccctgtgggg tgccttcctc cgttgctcgc cattgaccct cctccgacgt
     3001 cccggctgtg agccacttac gtatgagcct gattaccctc cggtcgccga tccgatgctc
     3061 tgtgaacctg atcagccatt cgtggctcac cctgtcgaag aactgactga tgtcggcatc
     3121 cagtacccag tttacgttag tgcgtaccag ccctgtggcc agtgcgtcca gtgcatcgtg
     3181 ctggcttcgc ccgggtctga acccgtatga gaaccccata aagtcgtttt catagactgc
     3241 gttcaggatt ttcaccagcg catactggac gatcttgtcc tccagcgagg cgatgccgag
     3301 cgggcgttgt tttccatccg cttttgggat gtagtgacgc ctgccgggct gcgccctgta
     3361 gctgccctga tgtagcctcc ggtgcagatc tgttatgttg ttcttcatgt ttccggcgta
     3421 gtccatccac ctgatgccat ccactccggc ggccgctttc ctgctcaggg agaggaatgc
     3481 ggcttccagt gcttcgactg tcagcaggtg gaacaatgct gtaaaccgtt ctttcttccg
     3541 ctgcttcgca gcttcccgca cgcgtgacag cctctgtgac atgctttccc ggctctgtgt
     3601 ccggcgcatg tgtggctgtt ccgcgttccc cttggccccg ctccttcgct ccactgactc
     3661 cgctcctttc gggttgttcg cctgcttcgc cgctactatg agcgagtccg acttctcctc
     3721 tccgtacatc accggctatg actcctcgtc ttcccggtgc gggccatctc cgacactggc
     3781 agatggtcag aggggagatc tcccggttcc cgcgtagaga tcgtattgac atgccagggt
     3841 ctcagacccc gccgggtcca tgtggcactc gcagtatcgc accctatgat gttgccttcc
     3901 gttaacagta caacgtcggc acccggtaat ttaatataca tttcgtggct caatggctgg
     3961 cctgtcaaca cccctgtcaa cgcttcgccc catacctcgc ggtatgcaac gcatgactcg
     4021 gggaccttgt ggattgctgg tccttcaatg gtcggggact ttcacccctt gatctctaac
     4081 cggtctcccg gcgcacactg tacatataca cagtataatt attaccttac tcctcccgtt
     4141 ttcaggtgtc aatatatgag attagttctc atcttcaggc acaaaaaagc gcccggagcc
     4201 acctgtaagc agatcgcgtc ttttcccgaa aatttctcat ggcaacaaat tttattattt
     4261 aagacattca tcacacttac atttcagatg gtatagacac atctgaaagc taactggcat
     4321 gaggagagga cctgcggtta aactgttcgg gaaacctgag acgcccggag gccactatga
     4381 cgcacctgaa actgactccc gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg
     4441 cactggccat gggcctgaaa gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc
     4501 gggaaagggt gcttcgtgcc attcaggacg aacgtgaagc caggcgggcg caggaaatgc
     4561 gtcgggagaa atacgaggct acggaaacca ccttcacctg gcagccacag cgccgccggt
     4621 aaggggtatt taccataacg tcgcattgcc cgcaccgctc agacggtctg agggcgtttc
     4681 cggcggctac gggataaaac aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg
     4741 gtaaattgta acccgctgca tgcctatgca cgcaactgca tggcgggccg tttggtgtaa
     4801 aaacgatacg ataagccgtg tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata
     4861 catgtaacat tttttactga caggtcatca acccgatgcg tttaataccc tttatcctgc
     4921 tggcattgtg cagcccctta ctcctgacgg cctgtaagca ttccaccacc cagccgccga
     4981 aaatttatac tctggataag tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg
     5041 ctgttgaaat gcagatgttt gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga
     5101 tatccataca acgtgatggc actctgaaca gcgccattgc tgaaggagga gatccgaaac
     5161 tctgtaaagc agcaatatca gccgttaccc acgccaaaat tccgccggca ccggatgaaa
     5221 atacctggca aatattcaaa aacgcccctc tggatttcag accatgaaag cttttactct
     5281 gacagtgctg gcggccatgt tcctcactcc ggtacatgcg ataccactaa aacccgtaac
     5341 cacactgcag ccaacctacc cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac
     5401 cgccacgttt gatattctgg acgatggacg agtggacaac gtgtcgctta ccggctgtca
     5461 cctttttttt cgggaaacgc tgacggcaat gaggcactgg ctatatcccg cagggcaccc
     5521 ggcaatgcga caggttataa attttgaatt cagtctgaaa aatgacggct tacaggatcc
     5581 gcgaccagtc accacccaaa agctcaatga tgatggttgc cgccagtaac aggtccttta
     5641 acccgcaggc tgcaatagtc ctggatgatg agtgagcctg tagcagccct gattagtgga
     5701 agcgaagctg aaaaatgggc agcaaaagcc gtatttctgg caaatagcgc cgccacggat
     5761 taaagtagtc atgtgtaata catatgacta caaaggggct tttatgtcat cagcaacatc
     5821 catcaggctc gatgaagaac ttaaggaccg gctgaaaacg ctggccgatg accggcaccg
     5881 ctcagcgcac gcgctaatgc tggaggcaat caccgaatac atcgaccggg aagagaagcg
     5941 cagccagtac ctgcgggacg gacaggccgc atggcagcac tatcaggaaa cgggtctgca
     6001 ccttactgcg gaagaagcgg aagcctggat aagtacctgg ggaactgaga acgagcagga
     6061 tgctccgcca tgccacaggt aacgatttcc gcgctggcac agcgcgattt acaacgcctt
     6121 caggactttc tcaaaaccaa aaatcggctg gcagccagaa aaggaggtga ggtgatcgtc
     6181 cgggctatcc agcaactgaa aacattgcca gacattggcc gcccggtgcc gtttctgccg
     6241 ctggaatata aggaactggt gatcgggttt ggcgacagtg gctatgtgat gctctaccgc
     6301 cacgaccggg aaatggacca gattgtgatc gtcaccgtca ggcatcagaa agaatccggg
     6361 tatccggggg cagacagcct ctaataaccg atgacggggg cgcaattcat tgcgtggccg
     6421 tcgccggttt gacaagcgca gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa
     6481 aattcagcga tacatttacc gtgagaaatt acacaccttt cacacccgat aaaatcaggg
     6541 gtatacagtg gcgaaaacat tgctcaacct ttcacaggca gcacaagccg cgggcattac
     6601 cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa
     6661 aaacaaccct gtcgtggatg tgtcagaact gatccgtgta tacggaaatg tgaatatccc
     6721 tgagaaacaa attcccggca tttcacaccg tgaaaataca caaaaaaatt tcccacagga
     6781 acagcttctg gcaatgcaaa aggaactggc cgatctccgg caggctgtca ccctgatgct
     6841 ggaagataaa acatcccggg aagaagaacg ccgccagcac gatgatgagc gccggaaact
     6901 ccaggccgaa gtggacagat tgacaacaga actgacacag aagaaaaaac gcttctggtc
     6961 cggctggttt agctgagaac ttaccgaagg gaatggagaa catgacgaag caggaaaaaa
     7021 ctgccctcaa tatggctaaa ttcattaagg atcagtcgct gctgttgctg gaaaagctga
     7081 acgagctgga catggatacc ccggccgaca tgtgcgaacg gcttcacgag gatgcggaaa
     7141 cactctggca ggtgatgcgg gataagctcg gggaagaata acagcaccgg ccatgccggt
     7201 gctggcgtta ttttttgccg aacggccacc accggcgggc gggtttgtcc tccggcagtt
     7261 caggcaccgg cacgttaaaa tagcggctgt tatattcccg catcagctcc gctttcagct
     7321 tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac ctcaatcatc gcgttaatct
     7381 gctccggtga gcacttcccg gcatcaacca cctgcacacc gttctcgatg gcgaaccgct
     7441 ccacctttcg atagaggtaa tcacacagct tcattcctgt gggtttgtcc cgttcccatt
     7501 gcggattttc accggtgaaa aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat
     7561 acatcccgat ggatttcatc atcgccagat gctgggcccg gtcgacaaaa cagaaaacat
     7621 acgccccggt ctgccaggcg agcaccacca tgtcttccag atgctcgctg gttggcataa
     7681 tgcagcgctc gccgtctttg tcttccacgt cctcaataaa taccatgtac agcgccataa
     7741 tccctcactc cgttgtcata aatttaaagc acgcgaacag cctctcccgg tccagctcca
     7801 ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc atccaccgcc agaatcgccc
     7861 gctgtacccg gacaagcgtc ggccggtcag ggcagatgta ccagatttca tcgatgccgt
     7921 tcgcccggcg gttgaacaga tacccggaca ccacttcctg ataccgcctg cggctcttga
     7981 cggtgcgctc tacctcaaat gccaccgttc tgccgtccag ctccgccagc gcgtccggca
     8041 ctttcatccc tttcagggcc atacggtgca ggtaccgcca gccggtggcc ccccggccct
     8101 ccatggagag gcgcagggac tgcacggcca gccggtgcgg cagctgcgcc gccgacaccc
     8161 ttgacggctg aaacggaata acgtccgtca gcggctcgtc ctcgtcgaac gagaacgcca
     8221 ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc gtcaaccggg tatttcgccc
     8281 ggaccagcag ccctttcgcc accagactgt ccaggaagcg gtaaaccggc tgtaccgccg
     8341 caatccccag ccgtcgcgca accagctccg ccgtggaaca cagctcttcc cgcagccagc
     8401 gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg cttcgcgtgg ctgacgttgt
     8461 actgctccag tgtcatcagt aaagccccgc aaaatcatca tccgcttttg gtccgccgcc
     8521 gtcggccgga ggcaggtact ccgccagcgg gtccgttttc gccagcaccg ggaaggtttt
     8581 cagcgaaatt tcccggcgct caacccgcag cgggctgctg aacgccagcc gcgccacgcc
     8641 cagcccggtc atcaccgcga acccgtcagg caggtgttgc agggtgttca cgtcaaacag
     8701 cggccgcgcc tccttctgga tgaagctgtc gccgctgatg tgctccacgt tccccgcctc
     8761 ccggctggcc cggcggcgct ccacgtccac ccggatctcc ccggtctggc ccgccgccca
     8821 ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac
     8881 cgtggttttc acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt cgcccagcga
     8941 ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac
     9001 gtacttcgac agcagatatt taatctcatc gagcatgatg ctcgcgtgcg gccacctccg
     9061 gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca aacagcatct tctgcacccg
     9121 gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg cagccgccgc cgttgattat
     9181 ccccgccaca tcgataccgc tgtccgtctg caggacgctc aggtcggcca cgttctccag
     9241 cttggtgatc agccccttca cgtccttttt cagggcctcc ggcaacgcgt acgccgccgc
     9301 cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct tcgccgcttt
     9361 ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc tcactcaggt taaacccggc
     9421 aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg aacagattca gctgcgccgg
     9481 tttccccggc cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga agacgtgcgg
     9541 cgcccacgcg tcccctttgg ggtcgaagta caccacagcg tccccgaaat cgcgtacgca
     9601 gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc ccgggcaggc cgaggatctg
     9661 aatattggtc ttgcgccagg tatcacgagg gaccagcacc ggctggccct gctcatcctt
     9721 acccagaaac accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg ccagcgcgtt
     9781 aaccggtacc ggctccccga aatgcaggtg gcggacgtct gtcatttctc cggtccgctg
     9841 gctgctgcgc cggctctcct gattcagttt cgcgttcacc gcctcgtcag cggccgcaaa
     9901 atgaccatca agcagcagga acgccagcag ccgcgccaga aggacgccgg cgacaagccc
     9961 tccggcacac caggctatcc cgaacaggtt agccggctgc agcaggtaat aatctgcgtt
    10021 cgcatagagg tccgggcgca ggacggtttc tccccagctc tcgataaaca cggcgacaac
    10081 cagccccccc agaagggcca gaccggcagc gccggtcagc cagtacgcgt agttccagac
    10141 atgtcgccgt gccctgacca tccgcccggg tgaatccagc gaggccagca ccgaaaatgg
    10201 cacagtccgg ttattatcgc gcttatagcg atcggcatac tcccgccgcg ccttatggct
    10261 cagccccaca aaacgcattc ggccggccca gagttcagcc agaccgaaaa acaggccgat
    10321 aaccagaaaa aaaccggcca gactgtaata ctcccggtac agccagctga cccacgggta
    10381 aatctgccgg gccagctccg ggatatgcca ctgccaggca agcgaatccg tattaaacat
    10441 gcaatctctc tccttcagac gacgggttta ccggcgtgat gcgcagcccc cgctgcacaa
    10501 gccgcggaca tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc
    10561 tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa
    10621 caacgagcac gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg
    10681 tgttccggcc cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag
    10741 cagctttgct gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt
    10801 gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg acagagccag
    10861 aaagagggga aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag gtcttttccc
    10921 ctgaagtcag ccacgcccca gcggggatga cttgcatttc tccgcaagga gaaaaaagca
    10981 acagagatag gttaacgtaa aatgttcagc gaaaaaccga catggttcac ctatcctgat
    11041 aattgatcgt cagggaatag aaagacttaa tcaggggaca atatcccaca tcagcgcgtc
    11101 cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc
    11161 tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt
    11221 ggataagcag gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc
    11281 cataggctcc gccctcctgc cagagttcac ataaacagac gctttttcgg tgcatctgtg
    11341 ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag
    11401 atccccaccg tttccggtag gtcgctccct cttgcgctct cctgttccga ccctgccgtt
    11461 taccggatac ctgtccgccg ttctcccttg cgggaagtgt ggcgctttct catagctcac
    11521 acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact
    11581 ccccgttcag cccgactgct gcgccttatc cggtaacttt catcttgagt ccaacccggg
    11641 aaagcacgca aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt
    11701 gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg
    11761 acagatttgg ttgctgtgct ctgcgaaagc cagttacccg gttaagcagt tccccaactg
    11821 acttaacctt cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt
    11881 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc
    11941 ccgtcagttc agaggaggac gatggtgcca
//
