LOCUS       NZ_CP082763            11970 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 36 plasmid pKP36_5, complete sequence.
ACCESSION   NZ_CP082763
VERSION     NZ_CP082763.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12389490
            Assembly: GCF_007955015.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Ruan,Z.
  TITLE     Whole-genome sequencing facilities tracking of a nosocomial outbreak
            of carbapenem-resistant Klebsiella pneumoniae sequence type 11
            strains carrying capsular loci KL64 and rmpA/rmpA2 genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2021) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Ruan,Z. and Jia,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2019) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Oxford Nanopore; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:45:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,950
            CDSs (total)                      :: 5,828
            Genes (coding)                    :: 5,646
            CDSs (with protein)               :: 5,646
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 68 of 182
            Pseudo Genes (incomplete)         :: 122 of 182
            Pseudo Genes (internal stop)      :: 24 of 182
            Pseudo Genes (multiple problems)  :: 32 of 182
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082763.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="36"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP36_5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-06-07"
                     /collected_by="Huiqiong Jia"
     gene            complement(join(11251..11970,1..12))
                     /gene="mobC"
                     /locus_tag="FTO14_RS29430"
                     /old_locus_tag="FTO14_029430"
     CDS             complement(join(11251..11970,1..12))
                     /gene="mobC"
                     /locus_tag="FTO14_RS29430"
                     /old_locus_tag="FTO14_029430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(12..1976)
                     /locus_tag="FTO14_RS29435"
                     /old_locus_tag="FTO14_029435"
     CDS             complement(12..1976)
                     /locus_tag="FTO14_RS29435"
                     /old_locus_tag="FTO14_029435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3460..3609)
                     /locus_tag="FTO14_RS29440"
                     /old_locus_tag="FTO14_029440"
     CDS             complement(3460..3609)
                     /locus_tag="FTO14_RS29440"
                     /old_locus_tag="FTO14_029440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3694..3951)
                     /locus_tag="FTO14_RS29445"
                     /old_locus_tag="FTO14_029445"
     CDS             complement(3694..3951)
                     /locus_tag="FTO14_RS29445"
                     /old_locus_tag="FTO14_029445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3961..5646)
                     /locus_tag="FTO14_RS29450"
                     /old_locus_tag="FTO14_029450"
     CDS             complement(3961..5646)
                     /locus_tag="FTO14_RS29450"
                     /old_locus_tag="FTO14_029450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5751..7238)
                     /gene="ltrA"
                     /locus_tag="FTO14_RS29455"
                     /old_locus_tag="FTO14_029455"
     CDS             complement(5751..7238)
                     /gene="ltrA"
                     /locus_tag="FTO14_RS29455"
                     /old_locus_tag="FTO14_029455"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7883..8128
                     /locus_tag="FTO14_RS29460"
                     /old_locus_tag="FTO14_029460"
     CDS             7883..8128
                     /locus_tag="FTO14_RS29460"
                     /old_locus_tag="FTO14_029460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8402..8773
                     /gene="tolA"
                     /locus_tag="FTO14_RS29465"
                     /old_locus_tag="FTO14_029465"
     CDS             8402..8773
                     /gene="tolA"
                     /locus_tag="FTO14_RS29465"
                     /old_locus_tag="FTO14_029465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8770..9135
                     /locus_tag="FTO14_RS29845"
     CDS             8770..9135
                     /locus_tag="FTO14_RS29845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9310..9588
                     /locus_tag="FTO14_RS29475"
                     /old_locus_tag="FTO14_029475"
     CDS             9310..9588
                     /locus_tag="FTO14_RS29475"
                     /old_locus_tag="FTO14_029475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9576..9890
                     /locus_tag="FTO14_RS29480"
                     /old_locus_tag="FTO14_029480"
     CDS             9576..9890
                     /locus_tag="FTO14_RS29480"
                     /old_locus_tag="FTO14_029480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10054..10482
                     /locus_tag="FTO14_RS29485"
                     /old_locus_tag="FTO14_029485"
     CDS             10054..10482
                     /locus_tag="FTO14_RS29485"
                     /old_locus_tag="FTO14_029485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10508..10687
                     /locus_tag="FTO14_RS29490"
                     /old_locus_tag="FTO14_029490"
     CDS             10508..10687
                     /locus_tag="FTO14_RS29490"
                     /old_locus_tag="FTO14_029490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10714..11244)
                     /locus_tag="FTO14_RS29495"
                     /old_locus_tag="FTO14_029495"
     CDS             complement(10714..11244)
                     /locus_tag="FTO14_RS29495"
                     /old_locus_tag="FTO14_029495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10646
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2779..3357
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11255..11967)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="98.0"
                     /evalue="1.31e-138"
ORIGIN
        1 ctccagtgtc atcagtaaag ccccgcaaaa tcatcatccg cttttggtcc gccgccgtcg
       61 gccggaggca ggtactccgc cagcgggtcc gttttcgcca gcaccgggaa ggttttcagc
      121 gaaatttccc ggcgctcaac ccgcagcggg ctgctgaacg ccagccgcgc cacgcccagc
      181 ccggtcatca ccgcgaaccc gtcaggcagg tgttgcaggg tgttcacgtc aaacagcggc
      241 cgcgcctcct tctggatgaa gctgtcgccg ctgatgtgct ccacgttccc cgcctcccgg
      301 ctggcccggc ggcgctccac gtccacccgg atctccccgg tctggcccgc cgcccactgc
      361 gcggactcct ggctggccgc gcgatagaac cagcggatcg gcgtgttatc cagtaccgtg
      421 gttttcacaa aatcagcggg cagatcctgc ccgcactgac cgaagtcgcc cagcgactgg
      481 tgcgcgagca gcaggttgca gtcccggctg cgcagcgtcc ccagcgcgtt cagcacgtac
      541 ttcgacagca gatatttaat ctcatcgagc atgatgctcg cgtgcggcca cctccggaac
      601 tcatcccgcg caatgatgat ttgcgcgcag cgggcaaaca gcatcttctg cacccggatc
      661 accgcctcat cgtccatcga gccgatgacg tacaggcagc cgccgccgtt gattatcccc
      721 gccacatcga taccgctgtc cgtctgcagg acgctcaggt cggccacgtt ctccagcttg
      781 gtgatcagcc ccttcacgtc ctttttcagg gcctccggca acgcgtacgc cgccgccagc
      841 acctgctgaa tgtttgcccc ctgcgggaac tgctccgcaa tcagcttcgc cgctttctgc
      901 tcgctgatgc ggtagtggtc cgccacgtcg ccggtctcac tcaggttaaa cccggcaacc
      961 agcagattct tcagggcgta ctggtcgaga tcgcggaaca gattcagctg cgccggtttc
     1021 cccggccgca gatccagcag ggtgaagtcc gggcagtgcg cccggaagac gtgcggcgcc
     1081 cacgcgtccc ctttggggtc gaagtacacc acagcgtccc cgaaatcgcg tacgcagcga
     1141 atgagcgcgt tggtggccat cgcgctcttg ccgctcccgg gcaggccgag gatctgaata
     1201 ttggtcttgc gccaggtatc acgagggacc agcaccggct ggccctgctc atccttaccc
     1261 agaaacaccg cctgctgttt ccgggcctgc tccgtggaaa aatcggccag cgcgttaacc
     1321 ggtaccggct ccccgaaatg caggtggcgg acgtctgtca tttctccggt ccgctggctg
     1381 ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc cgcaaaatga
     1441 ccatcaagca gcaggaacgc cagcagccgc gccagaagga cgccggcgac aagccctccg
     1501 gcacaccagg ctatcccgaa caggttagcc ggctgcagca ggtaataatc tgcgttcgca
     1561 tagaggtccg ggcgcaggac ggtttctccc cagctctcga taaacacggc gacaaccagc
     1621 ccccccagaa gggccagacc ggcagcgccg gtcagccagt acgcgtagtt ccagacatgt
     1681 cgccgtgccc tgaccatccg cccgggtgaa tccagcgagg ccagcaccga aaatggcaca
     1741 gtccggttat tatcgcgctt atagcgatcg gcatactccc gccgcgcctt atggctcagc
     1801 cccacaaaac gcattcggcc ggcccagagt tcagccagac cgaaaaacag gccgataacc
     1861 agaaaaaaac cggccagact gtaatactcc cggtacagcc agctgaccca cgggtaaatc
     1921 tgccgggcca gctccgggat atgccactgc caggcaagcg aatccgtatt aaacatgcaa
     1981 tctctctcct tcagacgacg ggtttaccgg cgtgatgcgc agcccccgct gcacaagccg
     2041 cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg ttcagctttc
     2101 accgcggagg ccagcctctt tccgccccat gcggaaaagg cgcagccccg gaggaacaac
     2161 gagcacgccc cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt ttccggtgtt
     2221 ccggcccccg tcagagttcg ccatagcgag cggctgacca cccgcaaaat caggagcagc
     2281 tttgctgctt cgtcaggtgc gtccagcacc gtggattttt gtgggtggtc aggcgtgagc
     2341 ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc agccggacag agccagaaag
     2401 aggggaaaag accgtgcgtc ggtggcgtcc agccagcgta agcaaggtct tttcccctga
     2461 agtcagccac gccccagcgg ggatgacttg catttctccg caaggagaaa aaagcaacag
     2521 agataggtta acgtaaaatg ttcagcgaaa aaccgacatg gttcacctat cctgataatt
     2581 gatcgtcagg gaatagaaag acttaatcag gggacaatat cccacatcag cgcgtccggt
     2641 gaccgtcttg caggtgccgg ggaaattgcg cctcctcgct cactcggtcg ctacgctccg
     2701 ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga ttccgtggat
     2761 aagcagggga ctaacatgtg aggcaaaacg gcagggccgc gccggtggcg tttttccata
     2821 ggctccgccc tcctgccaga gttcacataa acagacgctt tttcggtgca tctgtgggag
     2881 ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc
     2941 ccaccgtttc cggtaggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc
     3001 ggatacctgt ccgccgttct cccttgcggg aagtgtggcg ctttctcata gctcacacac
     3061 tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca agaactcccc
     3121 gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa cccgggaaag
     3181 cacgcaaaac gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta
     3241 gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga
     3301 tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt
     3361 aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc
     3421 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg cgatccccgt
     3481 cagttcagag gaggacgatg gtgccaccgt tccgccctga acatcccgga tataatttgc
     3541 ctgacatgcg gccagaagaa agccagcaac aatcaatata aaaagaaaaa tagtctttac
     3601 ctttttcata tactttccct gtatttaaag actccggcat tcatccctga acaccggaag
     3661 tcagtatttc aaatcatcct tgataatttt ttgttaccag ttaccgtccc gataatcaaa
     3721 agctatataa caattatcca catcaggttt tatttgattt ttaaaaaatg gctgtagtaa
     3781 aggaacccat tctttcttca aatcaaactc accattatta atattatcct ttaaaggcat
     3841 tcccaagctt tcaatgacag aaccatcgtc accaaagtct ttagaatact cttccccttt
     3901 aaactcttca gttttcttat caaaccatgt caaacgtaat ttaagcccca tatttacctc
     3961 tcaaagatat tttttaatgt tccgttttgg atctggccct ttaacctgct tacccgtttt
     4021 gggatcaaat gcgcctaggt gttcgccatc actggcacga tacccttcaa gctcaccgtg
     4081 ctgggaatcc cattcataaa ttttacgttt tttatcgcca taccatcggg cccgcttacc
     4141 accaccacct tgtttaggtg ttttaggatc acctcgcttt aactctccaa gtccctttat
     4201 gtcttctgtt ttaggtactg gatgataatc atggccgtag tctttagtcc ccttgcgagg
     4261 cttgttcttt tcctcattta acttattttc tgcatccttc gcttttttct ccttttgttt
     4321 ccggctctcc atcgccccgg taagtgcagc cttatctctg gataaggatt cattcattgc
     4381 gtttaaagat gcctgagccg cttttaattt ctcattctga ttctgaatat ccttatcaag
     4441 atagcgaacc tgatcctgcc acccggagcc atattcatga ggcgggttat aagtcacaaa
     4501 atcatcttta tttgcctctg cctcttttac ctttttatcg gcttcctgaa cagcagccct
     4561 tgcaccgggg agcgccttct ctgtcgatgc aatgcggctg tttaaggtcg caatattttt
     4621 atcttcggca tccagctcag ctttcgcttt atcatagtct cttttcagcc cctcttccgg
     4681 atgagcggcg tcccatgcct gctggcgacg cttttcttcc tcctgacgct gttttacctg
     4741 tgccggggta agaacatctg tcaccgacac ataaaccggc ttctgtccgg tctctttcgg
     4801 gaaacgaata acggcctcac gggagttacc accggctgta aaaccagccg gacgtgaatc
     4861 acctttttca gcaacaatgc cttttggcgg ggctttcgct gtcggaacac ctttcggtac
     4921 gctcacctgc agagacggga gacccggaat cgacacactg aatacccccg gacgttttgt
     4981 cggtttcgca tccaccacag ggacactcat tggccgacct gcgacaaccg caatatgctg
     5041 ccgctcatcc ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg cctggtccag
     5101 tggtaaagta cttgccggag tctccgtcac cgcatcggcc ggtaatgacg tcataatttt
     5161 tgacatcatt ttcggatcat cttttgcaat ctcagaaggc agcacaccgt agatcgcaat
     5221 cccccacaga ccaaacttaa acggcccctt cagggcagcc agcacatcag caacggcggc
     5281 cgacaacgca tcgccggaaa ctgataaagc cagtccttca gcccccggag tagccagggc
     5341 aggcagaccg aaggccatcg cagtggccga agactgtccg ccaccagagc taccgctatg
     5401 attgccgctg ttaccaccat taccaccggg tttactcccg ccgttaccaa actcccccgg
     5461 attatagtta tgaatatctc cattcggcgt atgcgtcgta ccccagcccg gcccgctgtt
     5521 tggatccgta ccaccacctg atgaagggcc acctttacca gacgtaccgt tcacattccc
     5581 actggcctga ccaccattat gtcccagacc tgaattaccc ggacctcgac cgtctccacc
     5641 actcataccg ttttcctctt tgacatttaa aacaataaat taaagccaac atgtcgcggt
     5701 agggataccc gttaccggat accccccgca cagatcccgg cgtgcgcgat ttacgcaccg
     5761 ggctcctgcc tcgggtgtct ggcggtgaac cgctccacag gccatggatg aagaacccga
     5821 acccttggta gccatgcggc tgccagtttg tttgctttcg tccaggtcgt atcatccttc
     5881 tggctcctgc gcctgagcgc ccggcgccag aggtttgtta cgtgtgtcct gaacttctgc
     5941 atggtgggga agttgcccgg taccgagtga tagttcaggt atccctgaac cactctcctg
     6001 agccattttc cctgttcggg gattgagtaa tgccagcgcc ttcgcagacc gtctttgatg
     6061 gctttcagag ttgccgtcat ccgatcccgg cgggtctttc gtatcagcat gaacctgccg
     6121 ttgcgatctt tcccgctgat gtgcgtgaac ccgaggaagt tgaacgtttc tggtttgcct
     6181 tttcccctga tggcacggtt ttcggcagcg aagcggccga actccatcag acgggttttc
     6241 tccgggtgaa ccgtgagtcc gaactccctc agtctgcgct gcatggctat acggaagcgc
     6301 cgggcatcgt atcgtttgtc gaacccgatg acgatgtcat cggcgtatct gaccattacc
     6361 acattgcctg tggcatagcg acgtcgccac tgatgcgccc acagatcgaa gacgtagtgg
     6421 aggtatatgt ttgccagcag cggtgagatg accgcaccct gtggggtgcc ttcctccgtt
     6481 gctcgccatt gaccctcctc cgacgtcccg gctgtgagcc acttacgtat gagcctgatt
     6541 accctccggt cgccgatccg atgctctgtg aacctgatca gccattcgtg gctcaccctg
     6601 tcgaagaact gactgatgtc ggcatccagt acccagttta cgttagtgcg taccagccct
     6661 gtggccagtg cgtccagtgc atcgtgctgg cttcgcccgg gtctgaaccc gtatgagaac
     6721 cccataaagt cgttttcata gactgcgttc aggattttca ccagcgcata ctggacgatc
     6781 ttgtcctcca gcgaggcgat gccgagcggg cgttgttttc catccgcttt tgggatgtag
     6841 tgacgcctgc cgggctgcgc cctgtagctg ccctgatgta gcctccggtg cagatctgtt
     6901 atgttgttct tcatgtttcc ggcgtagtcc atccacctga tgccatccac tccggcggcc
     6961 gctttcctgc tcagggagag gaatgcggct tccagtgctt cgactgtcag caggtggaac
     7021 aatgctgtaa accgttcttt cttccgctgc ttcgcagctt cccgcacgcg tgacagcctc
     7081 tgtgacatgc tttcccggct ctgtgtccgg cgcatgtgtg gctgttccgc gttccccttg
     7141 gccccgctcc ttcgctccac tgactccgct cctttcgggt tgttcgcctg cttcgccgct
     7201 actatgagcg agtccgactt ctcctctccg tacatcaccg gctatgactc ctcgtcttcc
     7261 cggtgcgggc catctccgac actggcagat ggtcagaggg gagatctccc ggttcccgcg
     7321 tagagatcgt attgacatgc cagggtctca gaccccgccg ggtccatgtg gcactcgcag
     7381 tatcgcaccc tatgatgttg ccttccgtta acagtacaac gtcggcaccc ggtaatttaa
     7441 tatacatttc gtggctcaat ggctggcctg tcaacacccc tgtcaacgct tcgccccata
     7501 cctcgcggta tgcaacgcat gactcgggga ccttgtggat tgctggtcct tcaatggtcg
     7561 gggactttca ccccttgatc tctaaccggt ctcccggcgc acactgtaca tatacacagt
     7621 ataattatta ccttactcct cccgttttca ggtgtcaata tatgagatta gttctcatct
     7681 tcaggcacaa aaaagcgccc ggagccacct gtaagcagat cgcgtctttt cccgaaaatt
     7741 tctcatggca acaaatttta ttatttaaga cattcatcac acttacattt cagatggtat
     7801 agacacatct gaaagctaac tggcatgagg agaggacctg cggttaaact gttcgggaaa
     7861 cctgagacgc ccggaggcca ctatgacgca cctgaaactg actcccgctg aagaaaagcg
     7921 cctggcggac gcctttgtcg ctcaggcact ggccatgggc ctgaaaggga acaaaacgct
     7981 gccaaaggcg gtaaaacaac aggtgcggga aagggtgctt cgtgccattc aggacgaacg
     8041 tgaagccagg cgggcgcagg aaatgcgtcg ggagaaatac gaggctacgg aaaccacctt
     8101 cacctggcag ccacagcgcc gccggtaagg ggtatttacc ataacgtcgc attgcccgca
     8161 ccgctcagac ggtctgaggg cgtttccggc ggctacggga taaaacaggc aacaaaccac
     8221 ccggaaaacc gccggaaacg cgcccggtaa attgtaaccc gctgcatgcc tatgcacgca
     8281 actgcatggc gggccgtttg gtgtaaaaac gatacgataa gccgtgttta tgtaaatgaa
     8341 cgtggccgta aagagtgaca aatatacatg taacattttt tactgacagg tcatcaaccc
     8401 gatgcgttta atacccttta tcctgctggc attgtgcagc cccttactcc tgacggcctg
     8461 taagcattcc accacccagc cgccgaaaat ttatactctg gataagtcat ctaaaatcag
     8521 tagctacgct gcggccatca agaccgctgt tgaaatgcag atgtttgaac cagaggccta
     8581 tgcgggaaaa atgtgcaccg tgcggatatc catacaacgt gatggcactc tgaacagcgc
     8641 cattgctgaa ggaggagatc cgaaactctg taaagcagca atatcagccg ttacccacgc
     8701 caaaattccg ccggcaccgg atgaaaatac ctggcaaata ttcaaaaacg cccctctgga
     8761 tttcagacca tgaaagcttt tactctgaca gtgctggcgg ccatgttcct cactccggta
     8821 catgcgatac cactaaaacc cgtaaccaca ctgcagccaa cctacccggt acaggccgca
     8881 gcaaaacgca tcgaaggtca ggtgaccgcc acgtttgata ttctggacga tggacgagtg
     8941 gacaacgtgt cgcttaccgg ctgtcacctt ttttttcggg aaacgctgac ggcaatgagg
     9001 cactggctat atcccgcagg gcacccggca atgcgacagg ttataaattt tgaattcagt
     9061 ctgaaaaatg acggcttaca ggatccgcga ccagtcacca cccaaaagct caatgatgat
     9121 ggttgccgcc agtaacaggt cctttaaccc gcaggctgca atagtcctgg atgatgagtg
     9181 agcctgtagc agccctgatt agtggaagcg aagctgaaaa atgggcagca aaagccgtat
     9241 ttctggcaaa tagcgccgcc acggattaaa gtagtcatgt gtaatacata tgactacaaa
     9301 ggggctttta tgtcatcagc aacatccatc aggctcgatg aagaacttaa ggaccggctg
     9361 aaaacgctgg ccgatgaccg gcaccgctca gcgcacgcgc taatgctgga ggcaatcacc
     9421 gaatacatcg accgggaaga gaagcgcagc cagtacctgc gggacggaca ggccgcatgg
     9481 cagcactatc aggaaacggg tctgcacctt actgcggaag aagcggaagc ctggataagt
     9541 acctggggaa ctgagaacga gcaggatgct ccgccatgcc acaggtaacg atttccgcgc
     9601 tggcacagcg cgatttacaa cgccttcagg actttctcaa aaccaaaaat cggctggcag
     9661 ccagaaaagg aggtgaggtg atcgtccggg ctatccagca actgaaaaca ttgccagaca
     9721 ttggccgccc ggtgccgttt ctgccgctgg aatataagga actggtgatc gggtttggcg
     9781 acagtggcta tgtgatgctc taccgccacg accgggaaat ggaccagatt gtgatcgtca
     9841 ccgtcaggca tcagaaagaa tccgggtatc cgggggcaga cagcctctaa taaccgatga
     9901 cgggggcgca attcattgcg tggccgtcgc cggtttgaca agcgcagcgc gtcagaaaac
     9961 cacatcctga acggaaaaac gggtaaaatt cagcgataca tttaccgtga gaaattacac
    10021 acctttcaca cccgataaaa tcaggggtat acagtggcga aaacattgct caacctttca
    10081 caggcagcac aagccgcggg cattaccaga agaacgctat acaatcatgt aaaacaggga
    10141 aaggttaccg tgtcacgtga cgggaaaaac aaccctgtcg tggatgtgtc agaactgatc
    10201 cgtgtatacg gaaatgtgaa tatccctgag aaacaaattc ccggcatttc acaccgtgaa
    10261 aatacacaaa aaaatttccc acaggaacag cttctggcaa tgcaaaagga actggccgat
    10321 ctccggcagg ctgtcaccct gatgctggaa gataaaacat cccgggaaga agaacgccgc
    10381 cagcacgatg atgagcgccg gaaactccag gccgaagtgg acagattgac aacagaactg
    10441 acacagaaga aaaaacgctt ctggtccggc tggtttagct gagaacttac cgaagggaat
    10501 ggagaacatg acgaagcagg aaaaaactgc cctcaatatg gctaaattca ttaaggatca
    10561 gtcgctgctg ttgctggaaa agctgaacga gctggacatg gataccccgg ccgacatgtg
    10621 cgaacggctt cacgaggatg cggaaacact ctggcaggtg atgcgggata agctcgggga
    10681 agaataacag caccggccat gccggtgctg gcgttatttt ttgccgaacg gccaccaccg
    10741 gcgggcgggt ttgtcctccg gcagttcagg caccggcacg ttaaaatagc ggctgttata
    10801 ttcccgcatc agctccgctt tcagcttctc gttcaccgcg ctttccccgt gccgctcgct
    10861 gaccacctca atcatcgcgt taatctgctc cggtgagcac ttcccggcat caaccacctg
    10921 cacaccgttc tcgatggcga accgctccac ctttcgatag aggtaatcac acagcttcat
    10981 tcctgtgggt ttgtcccgtt cccattgcgg attttcaccg gtgaaaaagt acgtggcttt
    11041 ttcgtcgctg ttgagccgct cgtcatacat cccgatggat ttcatcatcg ccagatgctg
    11101 ggcccggtcg acaaaacaga aaacatacgc cccggtctgc caggcgagca ccaccatgtc
    11161 ttccagatgc tcgctggttg gcataatgca gcgctcgccg tctttgtctt ccacgtcctc
    11221 aataaatacc atgtacagcg ccataatccc tcactccgtt gtcataaatt taaagcacgc
    11281 gaacagcctc tcccggtcca gctccaccgt tttgcgggcc tcgccggtct gtggattcac
    11341 gatttcatcc accgccagaa tcgcccgctg tacccggaca agcgtcggcc ggtcagggca
    11401 gatgtaccag atttcatcga tgccgttcgc ccggcggttg aacagatacc cggacaccac
    11461 ttcctgatac cgcctgcggc tcttgacggt gcgctctacc tcaaatgcca ccgttctgcc
    11521 gtccagctcc gccagcgcgt ccggcacttt catccctttc agggccatac ggtgcaggta
    11581 ccgccagccg gtggcccccc ggccctccat ggagaggcgc agggactgca cggccagccg
    11641 gtgcggcagc tgcgccgccg acacccttga cggctgaaac ggaataacgt ccgtcagcgg
    11701 ctcgtcctcg tcgaacgaga acgccacccc gtgcggcgtc agcccccaca cgctgacctg
    11761 acggccgtca accgggtatt tcgcccggac cagcagccct ttcgccacca gactgtccag
    11821 gaagcggtaa accggctgta ccgccgcaat ccccagccgt cgcgcaacca gctccgccgt
    11881 ggaacacagc tcttcccgca gccagcgcag ggtcagccgt gttttctccg cgttccgcgc
    11941 ctgccgcttc gcgtggctga cgttgtactg
//
