LOCUS       NZ_CP082758             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 37 plasmid pKP37_6, complete sequence.
ACCESSION   NZ_CP082758
VERSION     NZ_CP082758.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12389491
            Assembly: GCF_007954925.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ruan,Z.
  TITLE     Whole-genome sequencing facilities tracking of a nosocomial outbreak
            of carbapenem-resistant Klebsiella pneumoniae sequence type 11
            strains carrying capsular loci KL64 and rmpA/rmpA2 genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2021) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
REFERENCE   3  (bases 1 to 5596)
  AUTHORS   Ruan,Z. and Jia,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2019) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Oxford Nanopore; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:38:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,944
            CDSs (total)                      :: 5,822
            Genes (coding)                    :: 5,641
            CDSs (with protein)               :: 5,641
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 68 of 181
            Pseudo Genes (incomplete)         :: 121 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 32 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082758.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="37"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP37_6"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-06-18"
                     /collected_by="Huiqiong Jia"
     gene            complement(join(5582..5596,1..330))
                     /locus_tag="FTO11_RS29475"
                     /old_locus_tag="FTO11_029470"
     CDS             complement(join(5582..5596,1..330))
                     /locus_tag="FTO11_RS29475"
                     /old_locus_tag="FTO11_029470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(320..496)
                     /locus_tag="FTO11_RS29480"
                     /old_locus_tag="FTO11_029475"
     CDS             complement(320..496)
                     /locus_tag="FTO11_RS29480"
                     /old_locus_tag="FTO11_029475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1136..1294
                     /locus_tag="FTO11_RS30030"
     CDS             1136..1294
                     /locus_tag="FTO11_RS30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1652..1978
                     /locus_tag="FTO11_RS29485"
                     /old_locus_tag="FTO11_029480"
     CDS             1652..1978
                     /locus_tag="FTO11_RS29485"
                     /old_locus_tag="FTO11_029480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            2004..2549
                     /locus_tag="FTO11_RS29490"
                     /old_locus_tag="FTO11_029485"
     CDS             2004..2549
                     /locus_tag="FTO11_RS29490"
                     /old_locus_tag="FTO11_029485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2615..2965
                     /locus_tag="FTO11_RS29495"
                     /old_locus_tag="FTO11_029490"
     CDS             2615..2965
                     /locus_tag="FTO11_RS29495"
                     /old_locus_tag="FTO11_029490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3035..3301)
                     /locus_tag="FTO11_RS29500"
                     /old_locus_tag="FTO11_029495"
     CDS             complement(3035..3301)
                     /locus_tag="FTO11_RS29500"
                     /old_locus_tag="FTO11_029495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3285..3509)
                     /locus_tag="FTO11_RS29505"
                     /old_locus_tag="FTO11_029500"
     CDS             complement(3285..3509)
                     /locus_tag="FTO11_RS29505"
                     /old_locus_tag="FTO11_029500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(4176..4388)
                     /locus_tag="FTO11_RS29510"
                     /old_locus_tag="FTO11_029505"
     CDS             complement(4176..4388)
                     /locus_tag="FTO11_RS29510"
                     /old_locus_tag="FTO11_029505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4395..4904)
                     /locus_tag="FTO11_RS29515"
                     /old_locus_tag="FTO11_029510"
     CDS             complement(4395..4904)
                     /locus_tag="FTO11_RS29515"
                     /old_locus_tag="FTO11_029510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5155..5400
                     /locus_tag="FTO11_RS30035"
     CDS             5155..5400
                     /locus_tag="FTO11_RS30035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          complement(583..659)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          848..1245
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(4061..5592)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcccccttt tccagcacgg catgccgcag ccgctcgagt tcggcatcaa tggccatcag
       61 cgcggccacc acctgaaccc ggtcagcgcc tgaccactgg ccgccgttaa ttttccgggc
      121 gatctggttg aggttgttcc ccatcccggc aagctggcgc agcaaaaccg gatcgatgga
      181 cggcagccgc aacgaccgcg ccgggcgcgt atcgagacag gtttcccgca tccacaccgc
      241 cagttgcctt ccattgcagc gctccagcag ctgctggtac tccccatcgg tcacccggat
      301 acctaaaaaa cgggtacgct taaccgccat cctctcctcc gatatccgca aaatgtgacc
      361 gggctgaacg ctcaagctct gccatctgct cccggcggta atcttcctcc tgtgcctgac
      421 gcatcagatt tgccatcact gccggatgcc aggatctttt ttttacgacg agcggcttct
      481 tcttcggctt ctgcattatc cctccttcgc aaacgccgga cggcacggaa aagttttccg
      541 ggtcgctccg gcgttggaca agcgccagcg cgtcagtggt gttagcgggt ttcggggcgc
      601 agccctgaac cagtcacagc gtttacgctg tgtatactgg cttactatgt tggcatgaga
      661 gcacgattca tgaaagtgcc tcatgtggca ggtgtgatat gtggctcagg agcgtccgca
      721 ataccatgca cggacagatg aaatgacgcc tcctcgctca ctcggtagct gcgctcctgc
      781 cgttcggctg cggcgggcgt gaccgactca cggataacgg gataaagcaa aggcgctcaa
      841 atcagaaagg gccggagaaa aggatgcggc gttgccgttt ttccataggc tccgcctccc
      901 ctgacgagca tcacagaaat ggacgctcaa gtcagaggag gacgaaaccc gacaggactt
      961 aaagatgccg gtgatttctc tccggcggct ccctcgtgcg ctctcctgtt ccgaccctgc
     1021 cgtttaccgg atacctgttc cgcctttttc cctttcggga agcgtggcgc tttctcatag
     1081 ctcacgctgc tggtatctcg gctcggttta ggtcgttcgc tccaagctgg gctgtatgca
     1141 cgaacccccc gttcagcccg actgctgcgc cctttccggt aactatcatc ttgagtccaa
     1201 cccggacgga cacgcaaaaa cgccactggc agcagccatt ggtaactgga tatatacgag
     1261 agagatattg agaggtcttg aagtgggtgc ctaactgcgg ctacactgaa agaacagttt
     1321 tggtatctgc ggtctcgtat acccagttac ccggttaagc agttccccaa ctgacttaac
     1381 cttcgatcaa accgcctccc caggcggttt tttcgtttac agtgcaagag attacgcgca
     1441 gaaaaaaagg atctcaagaa gatcctttaa atgatcgtca gttctgttat ccccggagct
     1501 ccatccatcc cacggaaaaa cacccgccac tgatgccctc agcgcactgc acatcatgta
     1561 caaaccactc cctccctcct tttttattgc gtttcttaat gcgcaatggt agctttgtgc
     1621 gcaacaaaaa acgcaatagc aagggggggt aatgagagaa agaacaaata tcatgctaga
     1681 tataggatta aagcaatcct tacaacggct ttcgaataaa accaaaatga gtctaagtga
     1741 tcttattaac cagagattat caggttcact ttcagttgaa gaacgcattg gcgaaaagcc
     1801 tgaatggatg agaaaccttg atccctatct cgccaatcac ttttcaaata ttgagttacc
     1861 gctcccttca ccattaaaag aataccaaga aaaaatggat gagatagatt caattattaa
     1921 tgacatgatg gttctaaagg aatatttcag agctaagttt tacgacgaaa taaaataggc
     1981 acgctatacg gaggcggaga attatgtttt ttaaacggta tttaacggag aaggaaatat
     2041 acaaggcagc actcccaatt gttaggcttc aagggagagt taacacttca ttacgttttt
     2101 tatccaaata ctcagatgta aacaaaaatg gattaattgc attgcatctt aatttattac
     2161 attttatctc atacaacata cacggtgtag agtctctgaa aatcactcaa gcaggcataa
     2221 acaaagctgt acaacaaagc cctcacgcag atgaaataaa aaatattgcg caggcaatag
     2281 gacgcaaagg acctgagtac atcgaaggat taattagaaa cccatggggg aagaaaaatg
     2341 ataatccact aatatttata atgagcattg aagaaagtat aaaagaaacc cttaactcag
     2401 gttatatgtt atatagagta gctcccataa ctgagggtga agcagctgct ttcatggatg
     2461 ttcttaaaca attattatta tttgaggata attttcgcct ttttggtaga gacttggttt
     2521 ataaaataga aaaattaaaa atcagataac aaagtatggt gaacgaaaaa tgaaagagag
     2581 gatagtgaaa tgtgtaatac caagattctc aactatgtat tggttaattg cgttcctagc
     2641 atttatattc cccggtcgat cttttgctga cggttacacc gcttactacc gatgtaatgg
     2701 tactcaggaa gcatctctta ctgttaaaga taacaaacta agtatggttg taggtctttc
     2761 taagtttaat aggcaactgg atacttatag attcaaagaa aagggaatcg aagtagtaat
     2821 gcagagagct gcttctgatg ataactctga ggccataatg tcttttatta tgcctgacgt
     2881 taacaatatg accttattag ttaaaaataa cggctacatt atcgttaaga caacctgctg
     2941 gttaactaaa accgacaaac agtagaacac gtagttagtc gagtatttac cataatacct
     3001 gttgtcggca tcaaaaaagc ccgcatagcg ggcttcagtc gtaaacttcg cggcgatgtc
     3061 caatcgtggc ggcaaggata acaagctcgt catctcggat ctcacacaga acgcgataat
     3121 ctcccacccg atagcgccag aactcgccca gctcaccttt gagcggcttc cctgactggc
     3181 gaggatcggc agcaactgca atgcgtaaac tcataaaatc cacaatccgt cgtgcgttct
     3241 gtttgtccat cttgcgtaac gatttgagcg cccgatcgga atagttaatc gtccagacca
     3301 agtgacttcc tcacgtcttc agacgaatgc actgcttcat caccacggcg aacgcgtgca
     3361 gcagtttctg ctgaaagata ataatcctcc aggtcttcca gatgcgccag tattgcttcc
     3421 cgcgcataaa acgtctttgt tctgccggtt tgcttcgcca gcgagtccag acgggactca
     3481 atctcatcag ataaccggat agcaagcata taaacctccc gaacaccatt tgctatacat
     3541 gtatatcaac agtaccacaa tcaacctaaa agaacaaaaa ataatcttgt tagcccctct
     3601 tgataatccg taacgcggtc aatatatcta gcttgagggc tttcctctta actattgaaa
     3661 ggataacgat tatgggtctt aagccgggtc cgaaacgaat tgccgattca acaggggaac
     3721 ctgataaacg ccagcgtgac aataaaaaaa cgccgggaaa tactgacaaa ttgaaaccca
     3781 gcaaatcttc aaaaaaataa gtcagtagtt aaaagccctc cggggctttt ttgtatctgc
     3841 cgttcacgct gattaaacat aacacccgtt gcccgcaccg ccccggacgg gttcgccgcg
     3901 aacggggtgg cagtcaggta aaaccggcaa caaaccaccc gataaaccgc cccgttcgcg
     3961 acagataaat tttacctggc tgcatagcca tgcagccagg tgaatgacat ccggtttcag
     4021 cgtaaatgcc cgatgagaac cctgttttcc tgcatttcag cccagcgacg gaccgtcata
     4081 gcttcgctgc cgggaacgcg cttccgtcag ttccttttcg tgctgccgtt gctcgtgcgc
     4141 ccgttctgcg gccacggcca tctcatgacg ctgcactacc ggctcaaacg ctgcagttgc
     4201 ccgctcaagc gttccacctg ctgactcaag cgcgtcacgc gctcgctcag cgccgcgttc
     4261 tcccgttgca taagccctga cattttccgc cattccccga aggcgctctc ccactcgtcc
     4321 agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg tttttccagc
     4381 tcggtcatcg gcacttactc cttcaccagt ctcagcgcgt tctgcttcga ccgcccgttc
     4441 ccgaccgtcc agctctgcgt cctgtctacg gccgtcccct tcggcagcac cagaaaacgt
     4501 ccgttgctgt cttccaggta cgtcacgccc caggtcttcg cgttcagctt cgacagcgct
     4561 gcgttctgcc ggtcgatttc ggccagattt gacgctatca agctcccctg ataccacaac
     4621 accccgctca ggctggcgaa cagcagtcct gacaccatcg tgatggtcag ccatgtccgc
     4681 ccgaccatac gcatcacgct caggcggttc gactgcatgg ccgcgctcat accctgctcg
     4741 tgggcgcgga tggcggcgct gattctcttc ccgctttcgc tcagttccgc tctgacagac
     4801 ttctcgtgct cgctgaatgc ggctttcagc atttcgccgg tactctgctg ctgcgctttc
     4861 gatttctgct ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg ctcccttaag
     4921 tcggatgttt cgccctccgt ccgggtcggc aatgctgatg ctgctctttg tggtgcgcac
     4981 gacctcaaaa cctgcgcttt ccagcgcctc agtgacgtcc tgacgcgttt taagctcccc
     5041 tgacgaggcg agggccagta agccccgcgt aatagcctct gcggcttcct gcttcgtttt
     5101 gggtaacgaa gatggcgtca ccagcgcgcg ccggttctcc ggtgcgttcg ggtcgtgcag
     5161 ccccagtcgc ccgttcacca cggtctgcca cgcatcgatg cgcggacggt ctgcccggtc
     5221 gtagtacggc tggagacgcc tgccggtcag cagttccgtg ttcggtacca gaaaattcag
     5281 ttccagccgc cccttgtccc ggtgttcaac ccacagcacg ctgtactggt ctttatcgag
     5341 tccgggcatc agaacccgct cgaagctcgc catcagcttt tcacgctggc cgggcggtaa
     5401 atcctgttca gcaaaagaca ggacgccgga ggtgtacttt ttggcgtagg gcgaggcgtc
     5461 gataagctcc cggacctcct ccggctttcc ctgcaggacg atggccccgt cgcgctggcg
     5521 gtctttcccc agcagataat ccaccggccc ggcaccgccg cctcgccctc gcggatgaaa
     5581 cttaacgatc atcgtc
//
