LOCUS       NZ_CP082757            11969 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 37 plasmid pKP37_5, complete sequence.
ACCESSION   NZ_CP082757
VERSION     NZ_CP082757.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12389491
            Assembly: GCF_007954925.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Ruan,Z.
  TITLE     Whole-genome sequencing facilities tracking of a nosocomial outbreak
            of carbapenem-resistant Klebsiella pneumoniae sequence type 11
            strains carrying capsular loci KL64 and rmpA/rmpA2 genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Ruan,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2021) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
REFERENCE   3  (bases 1 to 11969)
  AUTHORS   Ruan,Z. and Jia,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2019) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Oxford Nanopore; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:38:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,944
            CDSs (total)                      :: 5,822
            Genes (coding)                    :: 5,641
            CDSs (with protein)               :: 5,641
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 68 of 181
            Pseudo Genes (incomplete)         :: 121 of 181
            Pseudo Genes (internal stop)      :: 24 of 181
            Pseudo Genes (multiple problems)  :: 32 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082757.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="37"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP37_5"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-06-18"
                     /collected_by="Huiqiong Jia"
     gene            complement(join(11882..11969,1..227))
                     /locus_tag="FTO11_RS29400"
                     /old_locus_tag="FTO11_029395"
     CDS             complement(join(11882..11969,1..227))
                     /locus_tag="FTO11_RS29400"
                     /old_locus_tag="FTO11_029395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(215..493)
                     /locus_tag="FTO11_RS29405"
                     /old_locus_tag="FTO11_029400"
     CDS             complement(215..493)
                     /locus_tag="FTO11_RS29405"
                     /old_locus_tag="FTO11_029400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(668..1033)
                     /locus_tag="FTO11_RS29815"
     CDS             complement(668..1033)
                     /locus_tag="FTO11_RS29815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(1030..1401)
                     /gene="tolA"
                     /locus_tag="FTO11_RS29415"
                     /old_locus_tag="FTO11_029410"
     CDS             complement(1030..1401)
                     /gene="tolA"
                     /locus_tag="FTO11_RS29415"
                     /old_locus_tag="FTO11_029410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(1675..1920)
                     /locus_tag="FTO11_RS29420"
                     /old_locus_tag="FTO11_029415"
     CDS             complement(1675..1920)
                     /locus_tag="FTO11_RS29420"
                     /old_locus_tag="FTO11_029415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2565..4052
                     /gene="ltrA"
                     /locus_tag="FTO11_RS29425"
                     /old_locus_tag="FTO11_029420"
     CDS             2565..4052
                     /gene="ltrA"
                     /locus_tag="FTO11_RS29425"
                     /old_locus_tag="FTO11_029420"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            4157..5842
                     /locus_tag="FTO11_RS29430"
                     /old_locus_tag="FTO11_029425"
     CDS             4157..5842
                     /locus_tag="FTO11_RS29430"
                     /old_locus_tag="FTO11_029425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5852..6109
                     /locus_tag="FTO11_RS29435"
                     /old_locus_tag="FTO11_029430"
     CDS             5852..6109
                     /locus_tag="FTO11_RS29435"
                     /old_locus_tag="FTO11_029430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            6194..6343
                     /locus_tag="FTO11_RS29440"
                     /old_locus_tag="FTO11_029435"
     CDS             6194..6343
                     /locus_tag="FTO11_RS29440"
                     /old_locus_tag="FTO11_029435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7826..9790
                     /locus_tag="FTO11_RS29445"
                     /old_locus_tag="FTO11_029440"
     CDS             7826..9790
                     /locus_tag="FTO11_RS29445"
                     /old_locus_tag="FTO11_029440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            9790..10521
                     /gene="mobC"
                     /locus_tag="FTO11_RS29450"
                     /old_locus_tag="FTO11_029445"
     CDS             9790..10521
                     /gene="mobC"
                     /locus_tag="FTO11_RS29450"
                     /old_locus_tag="FTO11_029445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            10528..11058
                     /locus_tag="FTO11_RS29455"
                     /old_locus_tag="FTO11_029450"
     CDS             10528..11058
                     /locus_tag="FTO11_RS29455"
                     /old_locus_tag="FTO11_029450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(11085..11264)
                     /locus_tag="FTO11_RS29460"
                     /old_locus_tag="FTO11_029455"
     CDS             complement(11085..11264)
                     /locus_tag="FTO11_RS29460"
                     /old_locus_tag="FTO11_029455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(11290..11718)
                     /locus_tag="FTO11_RS29465"
                     /old_locus_tag="FTO11_029460"
     CDS             complement(11290..11718)
                     /locus_tag="FTO11_RS29465"
                     /old_locus_tag="FTO11_029460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     BGC             1..10842
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6447..7024)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.11"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9791..10518
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tagagcatca catagccact gtcgccaaac ccgatcacca gttccttata ttccagcggc
       61 agaaacggca ccgggcggcc aatgtctggc aatgttttca gttgctggat agcccggacg
      121 atcacctcac ctccttttct ggctgccagc cgatttttgg ttttgagaaa gtcctgaagg
      181 cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc ggagcatcct
      241 gctcgttctc agttccccag gtacttatcc aggcttccgc ttcttccgca gtaaggtgca
      301 gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac tggctgcgct
      361 tctcttcccg gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc gctgagcggt
      421 gccggtcatc ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc ctgatggatg
      481 ttgctgatga cataaaagcc cctttgtagt catatgtatt acacatgact actttaatcc
      541 gtggcggcgc tatttgccag aaatacggct tttgctgccc atttttcagc ttcgcttcca
      601 ctaatcaggg ctgctacagg ctcactcatc atccaggact attgcagcct gcgggttaaa
      661 ggacctgtta ctggcggcaa ccatcatcat tgagcttttg ggtggtgact ggtcgcggat
      721 cctgtaagcc gtcatttttc agactgaatt caaaatttat aacctgtcgc attgccgggt
      781 gccctgcggg atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa aaaaggtgac
      841 agccggtaag cgacacgttg tccactcgtc catcgtccag aatatcaaac gtggcggtca
      901 cctgaccttc gatgcgtttt gctgcggcct gtaccgggta ggttggctgc agtgtggtta
      961 cgggttttag tggtatcgca tgtaccggag tgaggaacat ggccgccagc actgtcagag
     1021 taaaagcttt catggtctga aatccagagg ggcgtttttg aatatttgcc aggtattttc
     1081 atccggtgcc ggcggaattt tggcgtgggt aacggctgat attgctgctt tacagagttt
     1141 cggatctcct ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta tggatatccg
     1201 cacggtgcac atttttcccg cataggcctc tggttcaaac atctgcattt caacagcggt
     1261 cttgatggcc gcagcgtagc tactgatttt agatgactta tccagagtat aaattttcgg
     1321 cggctgggtg gtggaatgct tacaggccgt caggagtaag gggctgcaca atgccagcag
     1381 gataaagggt attaaacgca tcgggttgat gacctgtcag taaaaaatgt tacatgtata
     1441 tttgtcactc tttacggcca cgttcattta cataaacacg gcttatcgta tcgtttttac
     1501 accaaacggc ccgccatgca gttgcgtgca taggcatgca gcgggttaca atttaccggg
     1561 cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt tatcccgtag ccgccggaaa
     1621 cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata ccccttaccg
     1681 gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct cccgacgcat
     1741 ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc tttcccgcac
     1801 ctgttgtttt accgcctttg gcagcgtttt gttccctttc aggcccatgg ccagtgcctg
     1861 agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca ggtgcgtcat
     1921 agtggcctcc gggcgtctca ggtttcccga acagtttaac cgcaggtcct ctcctcatgc
     1981 cagttagctt tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa tgtcttaaat
     2041 aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt acaggtggct
     2101 ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca cctgaaaacg
     2161 ggaggagtaa ggtaataatt atactgtgta tatgtacagt gtgcgccggg agaccggtta
     2221 gagatcaagg ggtgaaagtc cccgaccatt gaaggaccag caatccacaa ggtccccgag
     2281 tcatgcgttg cataccgcga ggtatggggc gaagcgttga caggggtgtt gacaggccag
     2341 ccattgagcc acgaaatgta tattaaatta ccgggtgccg acgttgtact gttaacggaa
     2401 ggcaacatca tagggtgcga cactgcgagt gccacatgga cccggcgggg tctgagaccc
     2461 tggcatgtca atacgatctc tacgcgggaa ccgggagatc tcccctctga ccatctgcca
     2521 gtgtcggaga tggcccgcac cgggaagacg aggagtcata gccggtgatg tacggagagg
     2581 agaagtcgga ctcgctcata gtagcggcga agcaggcgaa caacccgaaa ggagcggagt
     2641 cagtggagcg aaggagcggg gccaagggga acgcggaaca gccacacatg cgccggacac
     2701 agagccggga aagcatgtca cagaggctgt cacgcgtgcg ggaagctgcg aagcagcgga
     2761 agaaagaacg gtttacagca ttgttccacc tgctgacagt cgaagcactg gaagccgcat
     2821 tcctctccct gagcaggaaa gcggccgccg gagtggatgg catcaggtgg atggactacg
     2881 ccggaaacat gaagaacaac ataacagatc tgcaccggag gctacatcag ggcagctaca
     2941 gggcgcagcc cggcaggcgt cactacatcc caaaagcgga tggaaaacaa cgcccgctcg
     3001 gcatcgcctc gctggaggac aagatcgtcc agtatgcgct ggtgaaaatc ctgaacgcag
     3061 tctatgaaaa cgactttatg gggttctcat acgggttcag acccgggcga agccagcacg
     3121 atgcactgga cgcactggcc acagggctgg tacgcactaa cgtaaactgg gtactggatg
     3181 ccgacatcag tcagttcttc gacagggtga gccacgaatg gctgatcagg ttcacagagc
     3241 atcggatcgg cgaccggagg gtaatcaggc tcatacgtaa gtggctcaca gccgggacgt
     3301 cggaggaggg tcaatggcga gcaacggagg aaggcacccc acagggtgcg gtcatctcac
     3361 cgctgctggc aaacatatac ctccactacg tcttcgatct gtgggcgcat cagtggcgac
     3421 gtcgctatgc cacaggcaat gtggtaatgg tcagatacgc cgatgacatc gtcatcgggt
     3481 tcgacaaacg atacgatgcc cggcgcttcc gtatagccat gcagcgcaga ctgagggagt
     3541 tcggactcac ggttcacccg gagaaaaccc gtctgatgga gttcggccgc ttcgctgccg
     3601 aaaaccgtgc catcagggga aaaggcaaac cagaaacgtt caacttcctc gggttcacgc
     3661 acatcagcgg gaaagatcgc aacggcaggt tcatgctgat acgaaagacc cgccgggatc
     3721 ggatgacggc aactctgaaa gccatcaaag acggtctgcg aaggcgctgg cattactcaa
     3781 tccccgaaca gggaaaatgg ctcaggagag tggttcaggg atacctgaac tatcactcgg
     3841 taccgggcaa cttccccacc atgcagaagt tcaggacaca cgtaacaaac ctctggcgcc
     3901 gggcgctcag gcgcaggagc cagaaggatg atacgacctg gacgaaagca aacaaactgg
     3961 cagccgcatg gctaccaagg gttcgggttc ttcatccatg gcctgtggag cggttcaccg
     4021 ccagacaccc gaggcaggag cccggtgcgt aaatcgcgca cgccgggatc tgtgcggggg
     4081 gtatccggta acgggtatcc ctaccgcgac atgttggctt taatttattg ttttaaatgt
     4141 caaagaggaa aacggtatga gtggtggaga cggtcgaggt ccgggtaatt caggtctggg
     4201 acataatggt ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag gtggcccttc
     4261 atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc atacgccgaa
     4321 tggagatatt cataactata atccggggga gtttggtaac ggcgggagta aacccggtgg
     4381 taatggtggt aacagcggca atcatagcgg tagctctggt ggcggacagt cttcggccac
     4441 tgcgatggcc ttcggtctgc ctgccctggc tactccgggg gctgaaggac tggctttatc
     4501 agtttccggc gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc tgaaggggcc
     4561 gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg agattgcaaa
     4621 agatgatccg aaaatgatgt caaaaattat gacgtcatta ccggccgatg cggtgacgga
     4681 gactccggca agtactttac cactggacca ggcgacggtt cgtgtcagac aacgggttgt
     4741 ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc caatgagtgt
     4801 ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt cgattccggg
     4861 tctcccgtct ctgcaggtga gcgtaccgaa aggtgttccg acagcgaaag ccccgccaaa
     4921 aggcattgtt gctgaaaaag gtgattcacg tccggctggt tttacagccg gtggtaactc
     4981 ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag aagccggttt atgtgtcggt
     5041 gacagatgtt cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa agcgtcgcca
     5101 gcaggcatgg gacgccgctc atccggaaga ggggctgaaa agagactatg ataaagcgaa
     5161 agctgagctg gatgccgaag ataaaaatat tgcgacctta aacagccgca ttgcatcgac
     5221 agagaaggcg ctccccggtg caagggctgc tgttcaggaa gccgataaaa aggtaaaaga
     5281 ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat atggctccgg
     5341 gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg agaaattaaa
     5401 agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata aggctgcact
     5461 taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa
     5521 gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc atgattatca
     5581 tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag gtgatcctaa
     5641 aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata aaaaacgtaa
     5701 aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca gtgatggcga
     5761 acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag atccaaaacg
     5821 gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt tgacatggtt
     5881 tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg gtgacgatgg
     5941 ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg gtgagtttga
     6001 tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa taaaacctga
     6061 tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac aaaaaattat
     6121 caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga gtctttaaat
     6181 acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga ttgttgctgg
     6241 ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg gaacggtggc
     6301 accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat caaaggatct
     6361 tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg
     6421 ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccggg taactggctt
     6481 tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc cggactttgg cgcacacttc
     6541 aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg
     6601 ctgccagtgg cgttttgcgt gctttcccgg gttggactca agtgaacagt taccggataa
     6661 ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg agcgaacgac
     6721 ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact tcccgcaagg
     6781 gagaacggcg gacaggtatc cggtaaacgg cagggtcgga acaggagagc gcaagaggga
     6841 gcgacctacc ggaaacggtg gggatcttta agtcctgtcg ggtttcgccc gtactgtcag
     6901 attcatggtt gagcctcacg gctcccacag atgcaccgaa aaagcgtctg tttatgtgaa
     6961 ctctggcagg agggcggagc ctatggaaaa acgccaccgg cgcggccctg ccgttttgcc
     7021 tcacatgtta gtcccctgct tatccacgga atctgtgggt aactttgtat gtgtccgcag
     7081 cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt gagcgaggag gcgcaatttc
     7141 cccggcacct gcaagacggt caccggacgc gctgatgtgg gatattgtcc cctgattaag
     7201 tctttctatt ccctgacgat caattatcag gataggtgaa ccatgtcggt ttttcgctga
     7261 acattttacg ttaacctatc tctgttgctt ttttctcctt gcggagaaat gcaagtcatc
     7321 cccgctgggg cgtggctgac ttcaggggaa aagaccttgc ttacgctggc tggacgccac
     7381 cgacgcacgg tcttttcccc tctttctggc tctgtccggc tggacgccgg ccacgccaga
     7441 aattcacccg gcccccgtca ggctcacgcc tgaccaccca caaaaatcca cggtgctgga
     7501 cgcacctgac gaagcagcaa agctgctcct gattttgcgg gtggtcagcc gctcgctatg
     7561 gcgaactctg acgggggccg gaacaccgga aaagaccgca ctggggtgcc gccttttcct
     7621 ctcacacgct ggggcgtgct cgttgttcct ccggggctgc gccttttccg catggggcgg
     7681 aaagaggctg gcctccgcgg tgaaagctga acgcaatcac cacggacgtg accgccgccg
     7741 ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc tgcgcatcac gccggtaaac
     7801 ccgtcgtctg aaggagagag attgcatgtt taatacggat tcgcttgcct ggcagtggca
     7861 tatcccggag ctggcccggc agatttaccc gtgggtcagc tggctgtacc gggagtatta
     7921 cagtctggcc ggtttttttc tggttatcgg cctgtttttc ggtctggctg aactctgggc
     7981 cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg cgggagtatg ccgatcgcta
     8041 taagcgcgat aataaccgga ctgtgccatt ttcggtgctg gcctcgctgg attcacccgg
     8101 gcggatggtc agggcacggc gacatgtctg gaactacgcg tactggctga ccggcgctgc
     8161 cggtctggcc cttctggggg ggctggttgt cgccgtgttt atcgagagct ggggagaaac
     8221 cgtcctgcgc ccggacctct atgcgaacgc agattattac ctgctgcagc cggctaacct
     8281 gttcgggata gcctggtgtg ccggagggct tgtcgccggc gtccttctgg cgcggctgct
     8341 ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac gaggcggtga acgcgaaact
     8401 gaatcaggag agccggcgca gcagccagcg gaccggagaa atgacagacg tccgccacct
     8461 gcatttcggg gagccggtac cggttaacgc gctggccgat ttttccacgg agcaggcccg
     8521 gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc tggtccctcg
     8581 tgatacctgg cgcaagacca atattcagat cctcggcctg cccgggagcg gcaagagcgc
     8641 gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg tggtgtactt
     8701 cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc cggacttcac
     8761 cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg atctcgacca
     8821 gtacgccctg aagaatctgc tggttgccgg gtttaacctg agtgagaccg gcgacgtggc
     8881 ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc agttcccgca
     8941 gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ttgccggagg ccctgaaaaa
     9001 ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg tcctgcagac
     9061 ggacagcggt atcgatgtgg cggggataat caacggcggc ggctgcctgt acgtcatcgg
     9121 ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc gctgcgcgca
     9181 aatcatcatt gcgcgggatg agttccggag gtggccgcac gcgagcatca tgctcgatga
     9241 gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc gcagccggga
     9301 ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg ggcaggatct
     9361 gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct ggttctatcg
     9421 cgcggccagc caggagtccg cgcagtgggc ggcgggccag accggggaga tccgggtgga
     9481 cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcacatca gcggcgacag
     9541 cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac acctgcctga
     9601 cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca gcccgctgcg
     9661 ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa cggacccgct
     9721 ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg attttgcggg
     9781 gctttactga tgacactgga gcagtacaac gtcagccacg cgaagcggca ggcgcggaac
     9841 gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc cacggcggag
     9901 ctggttgcgc gacggctggg gattgcggcg gtacagccgg tttaccgctt cctggacagt
     9961 ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc
    10021 gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga gccgctgacg
    10081 gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca ccggctggcc
    10141 gtgcagtccc tgcgcctctc catggagggc cggggggcca ccggctggcg gtacctgcac
    10201 cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg cggagctgga cggcagaacg
    10261 gtggcatttg aggtagagcg caccgtcaag agccgcaggc ggtatcagga agtggtgtcc
    10321 gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat ctgccctgac
    10381 cggccgacgc ttgtccgggt acagcgggcg attctggcgg tggatgaaat cgtgaatcca
    10441 cagaccggcg aggcccgcaa aacggtggag ctggaccggg agaggctgtt cgcgtgcttt
    10501 aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat tgaggacgtg
    10561 gaagacaaag acggcgagcg ctgcattatg ccaaccagcg agcatctgga agacatggtg
    10621 gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg
    10681 gcgatgatga aatccatcgg gatgtatgac gagcggctca acagcgacga aaaagccacg
    10741 tactttttca ccggtgaaaa tccgcaatgg gaacgggaca aacccacagg aatgaagctg
    10801 tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt
    10861 gatgccggga agtgctcacc ggagcagatt aacgcgatga ttgaggtggt cagcgagcgg
    10921 cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga atataacagc
    10981 cgctatttta acgtgccggt gcctgaactg ccggaggaca aacccgcccg ccggtggtgg
    11041 ccgttcggca aaaaataacg ccagcaccgg catggccggt gctgttattc ttccccgagc
    11101 ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gaagccgttc gcacatgtcg
    11161 gccggggtat ccatgtccag ctcgttcagc ttttccagca acagcagcga ctgatcctta
    11221 atgaatttag ccatattgag ggcagttttt tcctgcttcg tcatgttctc cattcccttc
    11281 ggtaagttct cagctaaacc agccggacca gaagcgtttt ttcttctgtg tcagttctgt
    11341 tgtcaatctg tccacttcgg cctggagttt ccggcgctca tcatcgtgct ggcggcgttc
    11401 ttcttcccgg gatgttttat cttccagcat cagggtgaca gcctgccgga gatcggccag
    11461 ttccttttgc attgccagaa gctgttcctg tgggaaattt ttttgtgtat tttcacggtg
    11521 tgaaatgccg ggaatttgtt tctcagggat attcacattt ccgtatacac ggatcagttc
    11581 tgacacatcc acgacagggt tgtttttccc gtcacgtgac acggtaacct ttccctgttt
    11641 tacatgattg tatagcgttc ttctggtaat gcccgcggct tgtgctgcct gtgaaaggtt
    11701 gagcaatgtt ttcgccactg tatacccctg attttatcgg gtgtgaaagg tgtgtaattt
    11761 ctcacggtaa atgtatcgct gaattttacc cgtttttccg ttcaggatgt ggttttctga
    11821 cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa ttgcgccccc gtcatcggtt
    11881 attagaggct gtctgccccc ggatacccgg attctttctg atgcctgacg gtgacgatca
    11941 caatctggtc catttcccgg tcgtggcgg
//
