LOCUS       NZ_CP082146             4135 bp    DNA     circular CON 11-SEP-2023
DEFINITION  Acinetobacter lwoffii strain ED23-35 plasmid pALWED1.8, complete
            sequence.
ACCESSION   NZ_CP082146
VERSION     NZ_CP082146.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251102
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Kurakov,A., Mindlin,S., Beletsky,A., Shcherbatova,N., Rakitin,A.,
            Ermakova,A., Mardanov,A. and Petrova,M.
  TITLE     The ancient small mobilizable plasmid pALWED1.8 harboring a new
            variant of the non-cassette streptomycin/spectinomycin resistance
            gene aadA27
  JOURNAL   Plasmid 84-85, 36-43 (2016)
   PUBMED   26896789
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4135)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
REFERENCE   4  (bases 1 to 4135)
  AUTHORS   Kurakov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2015) Laboratory of Molecular Genetics of
            Microorganisms, Institute of Molecular Genetics RAS, Kurchatov sq. 2
            23182, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.8-beta
            Genome Coverage              :: 106.0x
            Sequencing Technology        :: Illumina; Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/11/2023 06:40:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082146.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="ED23-35"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWED1.8"
                     /geo_loc_name="Russia"
     gene            join(4026..4135,1..13)
                     /locus_tag="ABWED_RS00005"
     CDS             join(4026..4135,1..13)
                     /locus_tag="ABWED_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_309459089.1"
                     /translation="MLDLFFGHELGVGETNKNLVKKFEIYKKRVLATILMKSCS"
                     /name="hypothetical protein"
     gene            988..1389
                     /locus_tag="ABWED_RS00010"
                     /old_locus_tag="ABWED_03553"
     CDS             988..1389
                     /locus_tag="ABWED_RS00010"
                     /old_locus_tag="ABWED_03553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_050041694.1"
                     /translation="MEIDSVTKQKAVRERTFKVRLSESEYQLLDANNPFGSIAKLLRQS
                     AVEKATQIHNSKADDDQKIKAKNVVESSYTKTERSLILELGRIGNNVNQIAKAINTDIA
                     GTGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1379..3193
                     /locus_tag="ABWED_RS00015"
                     /old_locus_tag="ABWED_03554"
     CDS             1379..3193
                     /locus_tag="ABWED_RS00015"
                     /old_locus_tag="ABWED_03554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_176453806.1"
                     /translation="MIVDFFRHGTGLSKGCLDYLLGEDRVREHAQVLSGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQQIMQNFEQCLFPGLDQDQYKVLWVQHQDKVNQV
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNAEHRLYDPDDPNNQQL
                     LINKKNLPKDVRDFKAQLHQRIYRAVANGEIADRQKLVEWLESNDVKVTRQTQKSISIE
                     NPYEGATRPIRLNGEVYEQGFRATGEYRQEVQQRIATYRGTTSERYRANVTDYQRQLKH
                     KSQYHSDRYPTVRREISAEPTEQLTADRKAARPVPSLATVQIEPFKANERADTDRRTAS
                     SEQKPTETAYYFEYGTDFGRCYFDYSHYCAQFYGEKQVQRDSDAQYDDQRETNASGSPE
                     PIGGQFDVQHMQTAKQQSPTLMYSDQQECRRMAERLHDSDGVLNDDRIRNTVIENHRRT
                     TAAITATTSAIAAATAEFRRDAKENYPSLISTIKGNGERTERLSANTKVIIDNTDAISR
                     IREKYGNVYRADQWQSQRDEPNYNELRGRTGENLNRTAVSLVRSSQSAAEFARNVRTIE
                     ANIIQMKERKMEMEQPQPKQDRGMSWGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3239..4027)
                     /locus_tag="ABWED_RS00020"
                     /old_locus_tag="ABWED_03555"
     CDS             complement(3239..4027)
                     /locus_tag="ABWED_RS00020"
                     /old_locus_tag="ABWED_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-II family aminoglycoside
                     nucleotidyltransferase AadA27"
                     /protein_id="WP_050041693.1"
                     /translation="MSETLQLEQLTESLQQLLGESLFAIYLYGSAVDGGLGPESDLDVL
                     VVVNQALTLHQRQQLAETLLKISYPIGAAQRALEVTIVLKEQILSGSYPLSYELQFGEW
                     LREELNQGALLRAHTDPDLSILLKKAQMHHRSLLGPSLTQWSTAIPEQHLWQAMADTYP
                     SIVAHWDEDADERNQILALCRIYFSLITNEIVPKDQAAHWVIAQLPSLHQPILQRMIQE
                     YKGEIRKQNWQQQHQALGPVVDFLSSKIDEQFNKKSSLIK"
                     /name="ANT(3'')-II family aminoglycoside
                     nucleotidyltransferase AadA27"
     ARG             complement(3243..4027)
                     /name="aadA27"
                     /gene="aadA27"
                     /gene_name="ANT(3'')-II family aminoglycoside
                     nucleotidyltransferase AadA27"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1380..3190
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagttgtagt taaaacctaa agaaaaaccc ctctgagtct tgcaacttag aggggttttg
       61 tcttaaattt ggtggtacgc agttatttga tgttaagagc atcaagtaac gaagatcagc
      121 agagttagac gctcgtactg atttcacctt tcggcttgta ccgcttttca gattctatca
      181 aattgataaa aaatggcaag cattgtatca gtaaaaagtc tttcgttact tgattctcgt
      241 tcatccaata agcaatacat agactgtatt cgagccttat cagcttctgt tcgccttttt
      301 taaaggaaga tatgcggtgc atgtggaggg aataagtgtc actggtgcat cgtagcgata
      361 gtgaagatcg agagattgga gctaaaccgt tacaggggaa tcccacacaa gcggagcgtt
      421 gagagtagtt gtataggttg gttaagttgc atgttggaga aatcctcgca gagataccac
      481 catacttcga cagggagcgc gtgggggaag gtacaaaact tttagtcctt gtctaccatg
      541 tatgtaaagc atggtgcgga tacgaccgat gcgtggctcc gtcagcctaa tcaggttaaa
      601 tcggcaatac ttaagggaag tgtcattttt tgaatgacag cccttaggga gagtattgcc
      661 gaaactctct caacgtccac catctgcctg agcgaagcga aggctgtggt ggcggtgaga
      721 aagagttgag cgtctttagt tttacaagat taatgccaat actttctaga gtttatatac
      781 cgagtcattt gatttaataa tttatagaac aaatttgtcc gtaattgata agcaaggata
      841 cacagttacc cctatttcac gatgttcaat agtgctaagc tgtgaccttg atcaggggct
      901 tcacccccga taccccgaaa accggcttaa agaaaaatca aagattttcc cttaaaccgg
      961 tttcataccg agaattggat tgatcaaatg gaaatagatt cagtaactaa acagaaagcg
     1021 gttcgggaac ggacgtttaa agtcagactt tcagaatcag agtaccaatt gcttgatgct
     1081 aataatcctt ttggttcaat cgcaaagttg ctacgtcaat cggcagtaga gaaagcaaca
     1141 cagattcata attcaaaagc agatgatgac caaaaaatta aagcaaaaaa cgttgttgaa
     1201 tcttcatata ccaagacaga acgttcttta attttagagt taggtcgcat aggtaataac
     1261 gtcaatcaga ttgccaaggc tattaatacg gatattgcag ggacaggtac ttttgacaaa
     1321 gtgaagttac ttcatttgtt gatttccata gatcagcaac ttcgggagtt acgtccagat
     1381 gatcgttgat ttttttagac atggcacagg tttatccaaa ggttgtctag attatttact
     1441 tggtgaagat cgagtgcgag aacatgcaca ggttttaagt ggtgatgtgg agctgacagc
     1501 acaactcatt gatagcagcc cttttgccaa gaaatatacc agcggttgtt tatcctttta
     1561 cgagcatgat ttatctgacc aggataaaca gcagatcatg cagaattttg agcagtgtct
     1621 ttttcctggc cttgatcaag atcagtataa agttctatgg gtacagcacc aagataaggt
     1681 gaaccaagtt actggagaga ctcggcttga gctgaatttt gtgattccta atgtggaact
     1741 ttcgacaggc aaacggctac agccatttta tgcaccggta gatctcgatc gagtagatct
     1801 gtttaagcag attaccaatg cggaacatag actgtatgac cctgatgacc cgaacaatca
     1861 gcagctttta atcaataaga agaacctccc aaaggacgtt cgggacttta aagcgcaatt
     1921 acatcaacgt atctatcgag ccgttgccaa tggtgaaatt gccgatcggc aaaaattggt
     1981 tgaatggctt gagtccaatg acgtcaaagt cacccgacag acccaaaaat caatatcaat
     2041 tgaaaatcca tatgagggcg caacacgtcc tatccgtttg aatggagagg tttatgagca
     2101 aggctttaga gctactggcg aatatcgaca agaagtacaa cagcgaattg caacgtaccg
     2161 aggaacaact tctgagcggt atcgagcaaa cgtcacagat tatcaaagac aacttaagca
     2221 caagagccaa tatcacagcg accgctatcc aacagtcaga cgagaaatta gcgcagaacc
     2281 tacagaacag ctcacagcag atcgaaaagc agctcgacca gtaccaagcc ttgctacagt
     2341 ccaaattgag ccgtttaaag caaatgaacg agcagataca gacagaagaa cagcaagttc
     2401 agaacaaaag cctactgaaa cagcttacta ctttgaatat ggcactgatt ttgggcgctg
     2461 ttattttgat tattctcatt attgcgctca gttttatggg gaaaagcaag tacagcgaga
     2521 ttcagacgct caatatgacg atcaacgaga aacaaatgcg agtggatcac ctgaaccgat
     2581 cggggggcag tttgacgttc agcacatgca gacagcaaaa cagcaaagcc caacgcttat
     2641 gtattcagat caacaagaat gcaggcgaat ggcagaacgg ttacatgatt ccgatggggt
     2701 attaaacgat gaccgaatta gaaacacagt tattgaaaat catcgaagaa caacagcagc
     2761 aattacagca acaacaagcg caattgcagc agcaacagca gagtttagac gtgatgcaaa
     2821 agaaaattat ccaagcctta tcagcacaat caaaggcaat ggagagcgta cagaaaggct
     2881 tagtgcaaac acaaaagtca ttatcgacaa cacagacgca atttcacgaa ttagagagaa
     2941 gtacggcaac gtataccgag cagatcaatg gcaaagtcag agagatgagc caaattacaa
     3001 cgaactacga ggacgtactg gagaaaatct caaccgtaca gcagtcagtc tcgtcagaag
     3061 tagccaaagc gcagcagaat ttgctcgaaa cgtccgaact attgaagcga atatcatcca
     3121 gatgaaggaa cggaaaatgg aaatggaaca gccccaacct aagcaagatc gagggatgag
     3181 ttgggggatg tagggcttat agaagcccgt cagctcaaaa tgcttggtta aaacactatt
     3241 acttgattag gctactcttc ttattaaatt gttcatcaat ttttgaacta aggaagtcaa
     3301 caacagggcc taaagcctga tgctgttgtt gccagttttg tttgcgtatc tcacctttat
     3361 attcttggat catgcgctgc aaaatgggtt gatgcagcga cggtaactga gctatcaccc
     3421 agtgtgcggc ttggtcttta ggcacaatct cattcgttat caagctaaaa taaatacggc
     3481 ataaggccaa aatctgatta cgttcatcgg catcctcatc ccaatgtgcc acaatagagg
     3541 gataggtgtc tgccattgcc tgccagaggt gctgttcagg aattgccgtt gaccactgtg
     3601 tcaaacttgg ccccaacaaa ctacgatgat gcatttgtgc tttcttcagc aaaatactca
     3661 gatcagggtc tgtatgtgcg cggagcaaag caccttggtt taactcctcc cgtaaccact
     3721 ccccaaattg tagttcgtag ctgagtggat aactgcctga gagaatttgc tctttaagta
     3781 cgatggtgac ttcaagtgca cgctgtgcag ccccaattgg atacgaaatt tttaataagg
     3841 tttctgccag ttgctgtctt tgatggagtg tcagtgcttg attcaccaca accaaaacat
     3901 ccagatcact ttctggacct agcccgccat caacagctga accatacaga taaatggcaa
     3961 agagggattc acccaaaagc tgctgtaaag attctgttaa ctgttcaagt tgcaaggttt
     4021 cggacatgct tgacctcttt ttcgggcatg agttaggagt aggagaaaca aataaaaatc
     4081 ttgttaaaaa atttgaaata tataaaaaaa gagtccttgc aacaattcta atgaa
//
