LOCUS       NZ_CP082132             2100 bp    DNA     circular CON 11-OCT-2023
DEFINITION  Escherichia coli strain NDM_11.16372 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP082132
VERSION     NZ_CP082132.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20857180
            Assembly: GCF_019844035.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Manyahi,J., Moyo,S.J., Blomberg,B., Langeland,N. and Robert,A.
  TITLE     NDM-5 Tanzania
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Manyahi,J., Moyo,S.J., Blomberg,B., Langeland,N. and Robert,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2021) Clinical Science, University of Bergen,
            Haukelands veien 208, Bergen 5020, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicyler v. v0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.3x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/11/2023 03:51:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,922
            CDSs (total)                      :: 4,802
            Genes (coding)                    :: 4,540
            CDSs (with protein)               :: 4,540
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 87 of 262
            Pseudo Genes (incomplete)         :: 170 of 262
            Pseudo Genes (internal stop)      :: 56 of 262
            Pseudo Genes (multiple problems)  :: 49 of 262
            Pseudo Genes (short protein)      :: 4 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082132.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NDM_11.16372"
                     /isolation_source="rectal swab from HIV infected patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="Tanzania:Dar es Salaam"
                     /lat_lon="6.7924 S 39.2083 E"
                     /collection_date="2018-02-03"
                     /collected_by="Joel Manyahi"
     gene            complement(join(1149..2100,1..14))
                     /locus_tag="K5P77_RS24320"
                     /old_locus_tag="K5P77_24320"
     CDS             complement(join(1149..2100,1..14))
                     /locus_tag="K5P77_RS24320"
                     /old_locus_tag="K5P77_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(879..1145)
                     /locus_tag="K5P77_RS24325"
                     /old_locus_tag="K5P77_24325"
     CDS             complement(879..1145)
                     /locus_tag="K5P77_RS24325"
                     /old_locus_tag="K5P77_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(1490..1721)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.571"
                     /coverage="100.0"
                     /evalue="9.53e-117"
ORIGIN
        1 ttatcttctg acattaagcc tgctccgatc tgggcaggct gtgatttgat gtggtatatt
       61 catcacgtac ctgtgcattg cgagccagta catgtcacca gagtttgccg ctgaggtgac
      121 acattcgaaa tgttggggag tgttgacgca ctccccaacg ccgattaaaa acgcctcaat
      181 tctgctccat tttctttgac ctgtcaaaac ccactcaaag cctcgtcatt actgggctaa
      241 accactattt catatccgcc atttatccct taagtataaa tacaaggatc acgcgcgaga
      301 attgcgcaaa aaataaccac aattttaacc cccaaaacaa gcacccccga acggcttacc
      361 ccaacctttt ccacacgccc gatcaccaaa acagggaata aaaacaaaac cctgtcttac
      421 aggtggatgg cctcacttcg tgaaaccatc caccccgcat aagtttttta atcggtggtg
      481 tcgtcaatca ctaaatcgga accccaaagg ggacccccac gatttagcat ttgacgagat
      541 taactctcgg agatggcatc atgcttttaa cggactttaa aaaacggtga acggatgcgg
      601 attttaatcc gtgtctgtga ggcgttgagc aacgagcaaa gcgaggcggt agaaagagag
      661 ccagcggcag gctcaggacg ataatccggg gaaaatgtac cactcgcaga gtgttaacat
      721 ttgacatcgg attgtctatc ctgcattggt gtcgagttgg caaggctgag gataatgctg
      781 aggataatgc tgaggataat gcaggataga cagagcacta gagattgcat gcaatctcgt
      841 acctatcacc tcagcctgcc gctggctctt ttccagaact aagaatgcgt tcttaaaaat
      901 ccaaacttga taccgtttaa atctattttc acaaaaagag tttttaccct ctcgtttgtt
      961 ttctgaatat ataaaattaa ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg
     1021 ccaaaactta atccatcaat aagcaaaata aggtttttaa acctgtcagg aatcaccgct
     1081 tcacttctta aaaaaaataa accaatagtc ttatttccat tcaggtattc aatattaata
     1141 ttcatacgtt aatccgattt atccttctca ggggcgcgtt tgtaacgccg tttacctgaa
     1201 tcccagacaa acgccgtccg cttcccgtca tcagtgccat cccctacatc gtcggcaatg
     1261 acaagatcct cattggtttc tcgatccaac tggaggacgt tttttagcgc cccaccggtc
     1321 gagataaaac ggcgcttgtg aagctgccgc gtcagctcaa gaaaccactc aggatcgttt
     1381 gccatatctt ccggtttaac ggagtatttc agcgtttcag caaccgcgct ttgcagttgc
     1441 tcggcaacgt tggccacaac ctcacctgtc ttagttttta ctgcccgaat atcgatattc
     1501 ggctcatagt tcacccgcaa gcaatcgcgc cagagttcta cccaacgttc gtgcttaacg
     1561 tagttcttcc ctttaaacca agaaggttgc accatcagca gacagtgaaa atgcggatgt
     1621 gcgctgccat ccttacctcg tgtcacctcc gtagccctga tccacccctg aacaggtgat
     1681 agctcctttc gcttttccat tcgcttaaac gccgcattca ttgctgtaag gactgttcca
     1741 agttcaccta tctcgcagtt cctgacagtt aacgtcagaa acaaccatcg ggaagacggg
     1801 taatccacaa cgattttcgg tagagcctga taaaaacggg cttgccacat gagggttctt
     1861 ctccactggc agacagggca atgacggaca cggcaaaatt gcgcactacg aagccgtagg
     1921 cgcgtttctc cggtttccat gatggtagac caaccaaaac gtaacaaacc gctacaggag
     1981 gccatacgga gtgcatagcg ctcgaattca gcagcagtct ggtagatacc cccgacagaa
     2041 tcggtaagcg tgcgctgggt atcccaaact gcatcacggg ggctgtagtc cgaaaggaat
//
