LOCUS       NZ_CP082102             6662 bp    DNA     linear   CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS15047 plasmid pMS15047B.
ACCESSION   NZ_CP082102
VERSION     NZ_CP082102.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934287
            Assembly: GCF_030515595.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:10:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,807
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,456
            CDSs (with protein)               :: 4,456
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 67 of 228
            Pseudo Genes (incomplete)         :: 139 of 228
            Pseudo Genes (internal stop)      :: 59 of 228
            Pseudo Genes (multiple problems)  :: 37 of 228
            Pseudo Genes (short protein)      :: 4 of 228
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082102.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS15047"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS15047B"
                     /geo_loc_name="Sweden"
                     /collected_by="Annelie Brauner"
                     /genotype="ST48"
     gene            <1..702
                     /locus_tag="K6128_RS24015"
                     /old_locus_tag="K6128_23810"
     CDS             <1..702
                     /locus_tag="K6128_RS24015"
                     /old_locus_tag="K6128_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(958..3084)
                     /gene="mobQ"
                     /locus_tag="K6128_RS24020"
                     /old_locus_tag="K6128_23815"
     CDS             complement(958..3084)
                     /gene="mobQ"
                     /locus_tag="K6128_RS24020"
                     /old_locus_tag="K6128_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_302350498.1"
                     /translation="MIESDNSAVASAAYRSGSLMLDERTGLTHDYTRKSGVAEAVILTP
                     ATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARETVLAFVRENFVS
                     QGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAETWRASWADHANR
                     ALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQNRLINRLNLEI
                     QISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSNAAWKLTSNAMS
                     MMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWAAPEYRSEQGKI
                     QSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQLLKREEELRRKE
                     QEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMTCYLLLHNRNGE
                     YTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSATFEQLLTQVCQ
                     TWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTLMPVMSSVDRET
                     RQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYAGYGVAVN
                     HDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEWRREEDAR
                     THATERRHDRGWSQ"
                     /name="mobQ"
     gene            3094..3282
                     /locus_tag="K6128_RS24025"
                     /old_locus_tag="K6128_23820"
     CDS             3094..3282
                     /locus_tag="K6128_RS24025"
                     /old_locus_tag="K6128_23820"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077794387.1"
                     /translation="MLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTV
                     VALHFRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3538..5697)
                     /gene="mobQ"
                     /locus_tag="K6128_RS24030"
                     /old_locus_tag="K6128_23825"
     CDS             complement(3538..5697)
                     /gene="mobQ"
                     /locus_tag="K6128_RS24030"
                     /old_locus_tag="K6128_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            5876..6130
                     /gene="mobC"
                     /locus_tag="K6128_RS24035"
                     /old_locus_tag="K6128_23830"
     CDS             5876..6130
                     /gene="mobC"
                     /locus_tag="K6128_RS24035"
                     /old_locus_tag="K6128_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2760..3066)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.730000000000001e-160"
     Typing          complement(5340..5698)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5699..5875
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..702
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3067..3282
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.28e-109"
     Typing          complement(962..3087)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.297"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3542..5784)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtcggatc taattgccta taaatccaat gcgctggttg aagccagtta taaattgacc
       61 ctacaggaac agcgattttt gttactttgc atcggtcgat tgaagtctgg ggctgatgca
      121 gaatctccaa aattgcaaaa gacaatgacc attacagctg cggaatattt cgattcattc
      181 cctgacatgg ggcggaaaaa tgccgaggtt cagttacagg aagcaattga tcgtttgtgg
      241 gacaggtcaa tcattctcaa ggatgacgag aaacgtgagg agttccgctg gatccagtat
      301 cgggcacagt acgctaaagg cgaggccaga gcacaaataa cattttctga cgcagtaatg
      361 ccttatttga cacaactaca agggcaattt actagagttg taatcaagaa tatatctaga
      421 ttatcaagaa gttacagtat aaggatatac gaactcctcc agcagttccg cagtactggc
      481 gaacgtatta tcgctctcga tgattttcgc tcaatgcttg atatagagca taaatatcaa
      541 acatataaat cacttaacca gcaattatta agaccctgca ttgacgaact gaacaaaaaa
      601 agcgacctgg ctgtaacggt tgagacgatc aaaaaggggc gcacggttgt agccctgcat
      661 tttcgcttca aggaagacaa acaaatcaag atgacgatct aaaatcgatt ttaaggcacc
      721 taaaacgctc cctgctgcgt tttttttgtg ttagtagtgc gaaggtacta ttgaagtgaa
      781 aaattgcgtc aaaagcgctc taatgcagcg aaaaaagaac ccccattttt tgggggcgga
      841 aaaacaaaac tatgcttgca ttatgtgtgt gcttcttgca ttcatccctg tcgatgaaca
      901 tcgaagaaca tcgacgaaca taacacgatg ttaaacaaaa caaaatattg ttcacattca
      961 ctgtgaccaa ccgcgatcat gtctcctctc ggtggcgtgt gttcgtgcat cttcctcgcg
     1021 ccgccattcg tcccaggtac gattaaaacc cagcgagcgg aacagatgat ccggctgttt
     1081 ttctggtgtg ttgacggcag caaaaagcgg tgcctcacgg gttttcagat gaaactccat
     1141 aagttccggc gcaggtgata tgtggctgtc tgcatcgtga tttacggcaa caccataccc
     1201 tgcataccgt aaccccttat cggtcagtgc actgatgacc agctggctgt tatcggctga
     1261 gggaacggca aacgcgggat acagcgtacc attaagccgg acaacaccgc ccagagagtc
     1321 atccacgccc atgaccagca aaagctgaag ctggcgggtt tcccggtcga cagaactcat
     1381 taccggcatc agcgtcggta gcagacgtcc ctgcgtaaaa tccaccttcc ttgctgccag
     1441 tgatggcatg gctggctggc ggtcttctcc aagacagaaa gactccggcc atgtcttcgg
     1501 ctggcggaga ttgcgggtat attgcggcca tgtctggcaa acctgcgtca gaagttgctc
     1561 aaaggtcgca gaggaataaa cacgttgcag tggtggatgt tgaccgattt cgcttcggtc
     1621 acagatataa ccactcctgt cacgcattaa atcaacacgc tcaaagattc tggtgcggta
     1681 tttctccagc tcgttccccc agagcgtgta ttctccgttc cggttgtgaa gcagaaggta
     1741 acaggtcatt tccccgttac caggcatggg ggcctcgcca taccccgtaa cgactgcgct
     1801 cagtacccct gaatccataa cggcattgtc atttttcctg agctgttccg ctcgtcgctc
     1861 cgcgcgacta cgttctgtat cctcctgctc cttccggcgc aattcctctt cacgtttaag
     1921 caactggact ctgagagctg caaggccgga tgccccccac tgctgccact gtttttcaaa
     1981 tgccgtctga ctcgcgggaa tgtcctgttt ctccaccttc cggtaatgct gtcgcaattt
     2041 ttccatttgc tggctcagcg actggatttt tccctgttcg ctacggtatt ccggtgccgc
     2101 ccattgcatc aggggaatac gggaaaaacc ggacgaaaac ggcgatgatt ttttcaggct
     2161 ggcagcatgg ctcatcgctt cctttcgttc ggtgttcagc tcttcccagc gtctgcgcgt
     2221 cgcctgcata tctgccatca tcgacatggc attactggtg agtttccatg ccgcattact
     2281 gtccttcggc aacgcttcga tgatgtcacg tagtgtgtac gaagttgcgt tgtcaggaag
     2341 cacgatattc agaaggtcag ccacacggcg ggcaggctct gtcagcccgc tgtttctgag
     2401 ttcggtacgg gaaatctgta tttccaggtt aagtctgtta atcagacgat tctgttcgcc
     2461 gcgttctgtt tcgataccgc gtgtttccat cgcacaggca gactttccga ggtgaatacc
     2521 aggcgttttc tcaagcccct gtcgctcgta tgaacgatgg tctatctctt cctgataacc
     2581 ggcgttcgcc agtgccctgt tcgcatggtc agcccatgat gcacgccacg tctcggcatg
     2641 tgtccggtcg ttccagtccc tgacctttcc accgaatcca gcaggtccga cagctctcgt
     2701 ggtcagcatg atgtgcgcat ggggatttgt tttatccatg tgatggaacg ccacatcagc
     2761 aatcatgccc tgactgacaa agttttctct gacaaaggcg agaacggttt cccgtgcggc
     2821 atcctgcggt agttcgcggg ggatggcgag ttcaatttcc cgcgcaagtt gtgagttctt
     2881 ccggcgttcg gccttctcaa cggcattcca gagttcggca cggttcgtac accaggcagg
     2941 ggctgtcgcg ggagtcagaa tcacagcttc agccacaccg cttttgcggg tgtagtcgtg
     3001 agtcagtccg gtacgctcat caagcatcag tgaaccggaa cgataggcag cagaagcaac
     3061 ggcgctatta tcgctctcga tgattttcgc tcaatgcttg atatagagca taaatatcaa
     3121 acatataaat cacttaacca gcaattatta agaccctgca ttgacgaact gaacaaaaaa
     3181 agcgacctgg ctgtaacggt tgagacgatc aaaaaggggc gcacggttgt agccctgcat
     3241 tttcgcttca aggaagacaa acaaatcaag atgacgatct aaaatcgatt ttaaggcacc
     3301 taaaacgctc cctgctgcgt tttttttgtg ttagtagtgc gaaggtacta ttgaagtgaa
     3361 aaattgcgtc aaaagcgctc taatgcagcg aaaaaagaac ccccattttt tgggggcgga
     3421 aaaacaaaac tatgcttgca ttatgtgtgt gcttcttgca ttcatccctg tcgatgaaca
     3481 tcgaagaaca tcgacgaaca taacacgatg ttaaacaaaa caaaatattg ttcacattca
     3541 ctgtgaccaa ccgcgatcat gtctcctctc ggtggcgtgt gttcgtgcat cttcctcgcg
     3601 ccgccattcg tcccaggtac gattaaaacc cagcgagcgg aacagatgat ccggctgttt
     3661 ttctggtgtg ttgacggcag caaaaagcgg tgcctcacgg gttttcagat gaaactccat
     3721 aagttccggc gcaggtgata tgtggctgtc tgcatcgtga tttacggcaa caccataccc
     3781 tgcataccgt aaccccttat cggtcagtgc actgatgacc agctggctgt tatcggctga
     3841 gggaacggca aacgcgggat acagcgtacc attaagccgg acaacaccgc ccagagagtc
     3901 atccacgccc atgaccagca aaagctgaag ctggcgggtt tcccggtcga cagaactcat
     3961 taccggcatc agcgtcggta gcagacgtcc ctgcgtaaaa tccaccttcc ttgctgccag
     4021 tgatggcatg gctggctggc ggtcttctcc aagacagaaa gactccggcc atgtcttcgg
     4081 ctggcggaga ttgcgggtat attgcggcca tgtctggcaa acctgcgtca gaagttgctc
     4141 aaaggtcgca gaggaataaa cacgttgcag tggtggatgt tgaccgattt cgcttcggtc
     4201 acagatataa ccactcctgt cacgcattaa atcaacacgc tcaaagattc tggtgcggta
     4261 tttctccagc tcgttccccc agagcgtgta ttctccgttc cggttgtgaa gcagaaggta
     4321 acaggtcatt tccccgttac caggcatggg ggcctcgcca taccccgtaa cgactgcgct
     4381 cagtacccct gaatccataa cggcattgtc atttttcctg agctgttccg ctcgtcgctc
     4441 cgcgcgacta cgttctgtat cctcctgctc cttccggcgc aattcctctt cacgtttaag
     4501 caactggact ctgagagctg caaggccgga tgccccccac tgctgccact gtttttcaaa
     4561 tgccgtctga ctcgcgggaa tgtcctgttt ctccaccttc cggtaatgct gtcgcaattt
     4621 ttccatttgc tggctcagcg actggatttt tccctgttcg ctacggtatt ccggtgccgc
     4681 ccattgcatc aggggaatac gggaaaaacc ggacgaaaac ggcgatgatt ttttcaggct
     4741 ggcagcatgg ctcatcgctt cctttcgttc ggtgttcagc tcttcccagc gtctgcgcgt
     4801 cgcctgcata tctgccatca tcgacatggc attactggtg agtttccatg ccgcattact
     4861 gtccttcggc aacgcttcga tgatgtcacg tagtgtgtac gaagttgcgt tgtcaggaag
     4921 cacgatattc agaaggtcag ccacacggcg ggcaggctct gtcagcccgc tgtttctgag
     4981 ttcggtacgg gaaatctgta tttccaggtt aagtctgtta atcagacgat tctgttcgcc
     5041 gcgttctgtt tcgataccgc gtgtttccat cgcacaggca gactttccga ggtgaatacc
     5101 aggcgttttc tcaagcccct gtcgctcgta tgaacgatgg tctatctctt cctgataacc
     5161 ggcgttcgcc agtgccctgt tcgcatggtc agcccatgat gcacgccacg tctcggcatg
     5221 tgtccggtcg ttccagtccc tgacctttcc accgaatcca gcaggtccga cagctctcgt
     5281 ggtcagcatg atgtgcgcat ggggatttgt tttatccatg tgatggaacg ccacatcagc
     5341 aatcatgccc tgactgacaa agttttctct gacaaaggcg agaacggttt cccgtgcggc
     5401 atcctgcggt agttcgcggg ggatggcgag ttcaatttcc cgcgcaagtt gtgagttctt
     5461 ccggcgttcg gccttctcaa cggcattcca gagttcggca cggttcgtac accaggcagg
     5521 ggctgtcgcg ggagtcagaa tcacagcttc agccacaccg cttttgcggg tgtagtcgtg
     5581 agtcagtccg gtacgctcat caagcatcag tgaaccggaa cgataggcag cagaagcaac
     5641 ggcgctgtag ccgctgtttc tggagatgat tttcatgctg agatgataaa tcgccatttc
     5701 tgccgtaatg cctttttgtg gttttctttc agccccgcag ggcgcacaca atcgaaacgg
     5761 agtggagatt gtataagtgc gcacttcgtg attttccgtg cattatgcca gctcagtttt
     5821 taaactacca gtgacgcgct aaaatgcgat ttttcatcgc gcacggagtt acatcatgag
     5881 cagaacactc gaacagaaga ttgctgatgc tgaggccaga ttacagcgcc tgaaggcgaa
     5941 gagcaggagt ctggacacag cgcagaaggt cattgtgggt gcagcattgc tggcaaaggt
     6001 cagaaagccg gaggaggtgc agttgcgtgc ctggttactc cagttcctga aggcagaggt
     6061 gacacgacag gctgatgtga cgcgcatact gccgctgatt aacgagctgg aagcgctgcc
     6121 aggacagtga ttgtatcgtc cgctcgccgc agtcccagcg accgagcgtt agcgagcgag
     6181 tgggcgagga agcggaagtg aatccgtcgc ctgattgagc acatacgcac ggtttttcgg
     6241 gaggtgatat gcgctgaacc cgcatgaata ctggatttcc acagggttgg agggggtgta
     6301 ttcggaactt gcaattctgc ttttttgcaa cgcattgttt ttattggttt tttgttttca
     6361 gcactccaag ttatccacag gctcgtcgtt aagtttttag atcttgtttt taagatcttg
     6421 tttttaatac ttgttttagc gccaaaattc cttatagaaa acaaaatgtt acaatgctat
     6481 aggtaatgtg atttaaggta aaggtaatat gatttaaggt gtttaggtgg gggaatttaa
     6541 ggtaaaggca atatgattta aggtaatatt cacagaaaaa gggatattaa aataaaaagg
     6601 cattgccttt cgttgagggt atgtttatct tttattcatt accttttggg gagggatgct
     6661 ct
//
