LOCUS       NZ_CP082093             8423 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS20645 plasmid pMS20645B, complete
            sequence.
ACCESSION   NZ_CP082093
VERSION     NZ_CP082093.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934290
            Assembly: GCF_030515535.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:26:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,074
            Genes (coding)                    :: 4,814
            CDSs (with protein)               :: 4,814
            Genes (RNA)                       :: 119
            rRNAs                             :: 7, 7, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 6 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 104 of 260
            Pseudo Genes (incomplete)         :: 160 of 260
            Pseudo Genes (internal stop)      :: 54 of 260
            Pseudo Genes (multiple problems)  :: 51 of 260
            Pseudo Genes (short protein)      :: 3 of 260
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082093.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS20645"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS20645B"
                     /geo_loc_name="Finland"
                     /collection_date="1975"
                     /collected_by="Benita Westerlund-Wilkstrom"
                     /genotype="ST537"
     gene            31..870
                     /locus_tag="K6132_RS25915"
                     /old_locus_tag="K6132_25665"
     CDS             31..870
                     /locus_tag="K6132_RS25915"
                     /old_locus_tag="K6132_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(1558..1791)
                     /locus_tag="K6132_RS25920"
                     /old_locus_tag="K6132_25670"
     CDS             complement(1558..1791)
                     /locus_tag="K6132_RS25920"
                     /old_locus_tag="K6132_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1798..2358)
                     /locus_tag="K6132_RS25925"
                     /old_locus_tag="K6132_25675"
     CDS             complement(1798..2358)
                     /locus_tag="K6132_RS25925"
                     /old_locus_tag="K6132_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(2355..3047)
                     /locus_tag="K6132_RS25930"
                     /old_locus_tag="K6132_25680"
     CDS             complement(2355..3047)
                     /locus_tag="K6132_RS25930"
                     /old_locus_tag="K6132_25680"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_302347714.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEIRELID
                     ATPFSKKYTSGVLSFAEKELPPGERERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLE
                     LNFVIPNMELQSGKRLQPYYDRADRPRINAWQTLVNHHYGLRDPNAPENRRTLVTPNNL
                     PKAKQEAAEAITRGLEALYRAGAIKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGAE
                     SQAKGGAV"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3037..3360)
                     /locus_tag="K6132_RS25935"
                     /old_locus_tag="K6132_25685"
     CDS             complement(3037..3360)
                     /locus_tag="K6132_RS25935"
                     /old_locus_tag="K6132_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3424..3615
                     /locus_tag="K6132_RS25940"
                     /old_locus_tag="K6132_25690"
     CDS             3424..3615
                     /locus_tag="K6132_RS25940"
                     /old_locus_tag="K6132_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5388..5621)
                     /locus_tag="K6132_RS25945"
                     /old_locus_tag="K6132_25695"
     CDS             complement(5388..5621)
                     /locus_tag="K6132_RS25945"
                     /old_locus_tag="K6132_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5628..6170)
                     /locus_tag="K6132_RS25950"
                     /old_locus_tag="K6132_25700"
     CDS             complement(5628..6170)
                     /locus_tag="K6132_RS25950"
                     /old_locus_tag="K6132_25700"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_230905694.1"
                     /translation="MGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDA
                     ISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTALSVTGG
                     IFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGVKG
                     ENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6223..6657)
                     /locus_tag="K6132_RS25955"
                     /pseudo=""
     CDS             complement(<6223..6657)
                     /locus_tag="K6132_RS25955"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6866..7189)
                     /locus_tag="K6132_RS25960"
                     /old_locus_tag="K6132_25710"
     CDS             complement(6866..7189)
                     /locus_tag="K6132_RS25960"
                     /old_locus_tag="K6132_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7252..7443
                     /locus_tag="K6132_RS25965"
                     /old_locus_tag="K6132_25715"
     CDS             7252..7443
                     /locus_tag="K6132_RS25965"
                     /old_locus_tag="K6132_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3704..3788)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(7532..7616)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          3799..4354
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.907"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          7627..8182
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.907"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1461..2392)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2638..3047)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.161"
                     /coverage="83.0"
                     /evalue="6.19e-83"
     Typing          complement(5291..6876)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcatagtg agcatacaca ggagccgaac ttgcttacac tgttcatctt ttttgtttta
       61 ttaatagctg catgtttctt ttgttttgct ccaccacgaa gaggatatga cagaaatgag
      121 atcattccat acaaaataaa actatcaata aataaatatc gcctttacat atactcatcg
      181 ggaaaggtaa gacaatacct tttattttta gttattttat cactgtacta ctccatagcg
      241 gaaccattta agtctgagtt aataaaaaac atatcatatt cactgatggc tgcatttatt
      301 tttgatacag ggcttaattt tagcaaggag aatataacta aaggagtgat ttcaacaagg
      361 tggcataatg acctatattc atcatttgag agaatgaaag caattaataa aatttactac
      421 ccatccaaca aagagataaa cacagaaggt ctatccaaag ccatcacatc atctcttttc
      481 aatgatgacg cgaactcgtt cgcaaagaga gattttcgct tgatgtggga cttatcttct
      541 gaaaaatatc tttcctataa agaaataata ataagaaaag gcgataagct tgacgctgta
      601 tgcttgagat ttataaatga tgactacaag tttttagtta atttcaacag agatgaagag
      661 gttttcaaat actttccatc tataatgcaa ccttcactca aaacatatag agcgttatca
      721 agactagtaa actcaatcaa agatccttct agatttaagt tcacgacaga gtcattagaa
      781 atggagttac tagaatactt agagttaaga aatgaattat tcaatgacat agaagaagta
      841 atgggtagct atgctcagag ggcaccatag ttcatgatag ccctcgatag tacttactta
      901 tatttttaga gaggtttaag tgtaagtaga gggagtaaca cctggttttt cagcatccac
      961 tccaatgaat gctgacaccg atacgcgata attctcttta tcgtagcaaa atcatcctcc
     1021 agattgcggt tgagaatagg ttcatggtgc gcaatacgat tgcgcagttt tctcacggga
     1081 cttgttagca gtaatgcttc cccccgggga caaacgacag agatatacaa caaaggcccg
     1141 caattaaaat actccgtatt taccataacg tcgcattgcg cgtaccgctt aaacgcgttc
     1201 agcgcgatca cggcagcaga caggtaaaaa cagcaacaaa ccacccgaaa aactgccgcg
     1261 atcgcgcctg gtaaatttta acctcatgca tagttatgcg cgaaactgaa tgacgatcag
     1321 tttcgcacgt tccagccgtc gaacggggca aaatggggag gtttggatat tgttaatgct
     1381 aatgtacgta cattaacatt ggcgccatcc gggtagggcg tgccacaaac tggctcgatt
     1441 tcagggggta ccccggtcac atccccattg acgggccacg ccagatccgc gcttcgggtt
     1501 cctgctcttc atgccagcgc cgggcaagct cctgcgcctg acgctcctgt tcatccctta
     1561 tctgttgttc gtgataaata accgactcaa gcggtcgact tgtccggcta atagctgcac
     1621 ctgctgactc aagttcacga cacgctcgtt cagtatcgcg ttgttctgtt gcgtaaccgc
     1681 gtgcatcttc tgcaattctg cgaaggcgct ctcccactcg ttcagccgcc gcatatagtc
     1741 ctgttgcaaa tgctctaagg cgttcagcaa atgcccttcc agttccgtca tactcgttta
     1801 ctccctgacc agcctcacgg cgttcttgtc gcccaccgtc caggtgtttt cccctttcac
     1861 cccgtccggc agcaccagaa atttcccgtt ctcgccattg acgaacgtca ccccccaggt
     1921 ttttgcctcg agtttcgcca gcgtgttttc ctgctctctg atggtcaaga tatttttcgc
     1981 aatcctgctt tcctgccact gaatcagccc ccagcacccc aggaaaatgc cgcccgtgac
     2041 gctgagcgcg gtcagcgccg gaaacagtac ccgccctttg atcacctgcc agagcaggtt
     2101 tttctgctgc cgggacagtt cggcctctgt ggtgctgagc tgcgtgctca cggtggtgct
     2161 cagcgtatcc agttgctgtt tcacggatgt tgtatgttcg ctgatagcgt ctctgatttt
     2221 ctgcctgttt aagctcagtg ccttgtctac agactcttct agcttcctga actcgctgtt
     2281 cagcatgttc tcggtagaag cggctcgctc tttcagtttc ttctcgaagt ccgttcccat
     2341 ttgtaaaaga ttgctcatac agcgccccct ttagcctgag attccgcccc cccctccggg
     2401 tcggcaatac tgatactgct tctggttgtc ctcaccacct caaaacctgc cgctgtgagc
     2461 gcctcagtga catcctgacg cgtttttatt gctccggcac ggtaaagcgc ctcaagaccc
     2521 cgtgtaatcg cttctgcggc ctcctgtttc gcttttggca gattattcgg tgtgacaagc
     2581 gtccgtctgt tctccggtgc gttcgggtca cgcagcccgt aatgatgatt aaccagcgtc
     2641 tgccaggcat ttatcctcgg cctgtccgca cggtcgtagt acggctggag gcgcttaccg
     2701 ctctgtagtt ccatgttcgg aatgacaaaa ttcagttcga gccgcccctt atcctggtgt
     2761 tccacccaga gaatgctgta ctggtctttt tcgagaccgg gcataagaac acgctcaaag
     2821 ctcgtcatca ctctttcgcg ttcacccggc ggtaactcct tctccgcgaa cgacagaacg
     2881 cctgacgtgt atttcttcga aaatggcgtg gcatcaatga gttcccggat ttcttccggg
     2941 ttaccccgga gcaccgttgc gccttcgcgg ttacgctccc gccccagcag gtaatcgacc
     3001 gggccacttc caccgccttt ccccctggca tgaaatttaa ctatcatccc gtcccccctg
     3061 ttccctgaca gcctgccgca actggcgcaa ttcaccctcg atggccatca gagcggccac
     3121 cacatgaacc cggtcaatcg aagaccagtg gccgctgttc accttccggg ctgtctggtt
     3181 cagattgttc ccgatggcag ccagatgccg cagcaatggc ggcgatagtg tcggcagttt
     3241 tcctgtgcgc gcaaccggct cccccagaca gacccgccgc atccattctg ccagtcgttt
     3301 accctcgcag cgttccagta accgggcgtg ctcctcatcg ctaacccgta tcgtgagcat
     3361 cttctcgcgt ttcatcggta tcattccccc ctaaaaaatc ccctattaag gagccttaag
     3421 accatgacca aacaggaaaa aacagccctt aacatggccc gctttatcag aagccagacg
     3481 ctgaccctgc tggaaaaact caacgagctg gacgcggacg aacaggcaga catctgcgaa
     3541 tcacttcacg accacgccga cgaactctac cgtagttgcc tcgcacgctt cggcgatgac
     3601 ggtgaaagca actgaaacat gcagctcccg gagacgttca cagcttgcct gtgagcggat
     3661 gccgggagca gacaagcccg tcagggcgcg tcagcgggtt ttagcgggtg tcggggcgca
     3721 gccctgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gtggcatcag
     3781 tgcggattat aagaaaagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     3841 gaaaatgccg catcaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg
     3901 ttcggctgcg gcgagcggta tcagctcact caaagacggt gatgcggtta tccacagaat
     3961 caggggataa cgccaaaaga aacatgtgag caaaaggcca ggaacaggaa gaaggccgcg
     4021 ccgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa cagatgctca
     4081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt ggtatctcgg cccggtgtag
     4261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4321 ttatccggta actatcgtct tgagtccaac ccggaaagac acgtaaaaac gccactggca
     4381 gcagccattg gtaaccgttt agaggagtta gtcttgaagt tatgcgccgg ttaaggctaa
     4441 actgaaagga cagatttggt ggctgtgctc ctccaagcca gttaccttgg ttcaaagagt
     4501 tgctgctctg agaaccttcg aaaagccgcc ttgctgggcg gttttttcgt ttacggacaa
     4561 gactaccttc aggatcgtta gatctcaaga caatcatgca tagtgagcat acacaggagc
     4621 cgaacttgct tacactgttc atcttttttg ttttattaat agctgcatgt ttcttttgtt
     4681 ttgctccacc acgaagagga tatgacagaa atgagatcat tccatacaaa ataaaactat
     4741 caataaataa atatcgcctt tacatatact caggtttttc agcatccact ccaatgaatg
     4801 ctgacaccga tacgcgataa ttctctttat cgtagcaaaa tcatcctcca gattgcggtt
     4861 gagaataggt tcatggtgcg caatacgatt gcgcagtttt ctcacgggac ttgttagcag
     4921 taatgcttct cccccgggga caaacgacag agatatacaa caaaggcccg caattaaaat
     4981 actccgtatt taccataacg tcgcattgcg cgtaccgctt aaacgcgttc agcgcgatca
     5041 cggcagcaga caggtaaaaa cagcaacaaa ccacccgaaa aactgccgcg atcgcgcctg
     5101 gtaaatttta acctcatgca tagttatgcg cgaaactgaa tgacgatcag tttcgcacgt
     5161 tccagccgtc gaacggggca aaatggggag gtttggatat tgttaatgct aatgtacgta
     5221 cattaacatt ggcgccatcc gggtagggcg tgccacaaac tggctcgatt tcagggggta
     5281 ccccggtcac atccccattg acgggccacg ccagatccgc gcttcgggtt cctgctcttc
     5341 atgccagcgc cgggcaagct cctgcgcctg acgctcctgt tcatccctta tctgttgttc
     5401 gtgataaata accgactcaa gcggtcgact tgtccggcta atagctgcac ctgctgactc
     5461 aagttcacga cacgctcgtt cagtatcgcg ttgttctgtt gcgtaaccgc gtgcatcttc
     5521 tgcaattctg cgaaggcgct ctcccactcg ttcagccgcc gcatatagtc ctgttgcaaa
     5581 tgctctaagg cgttcagcaa atgcccttcc agttccgtca tactcgttta ctccctgacc
     5641 agcctcacgg cgttcttgtc gcccaccgtc caggtgtttt cccctttcac cccgtccggc
     5701 agcaccagaa atttcccgtt ctcgccattg acgaacgtca ccccccaggt ttttgcctcg
     5761 agtttcgcca gcgtgttttc ctgctctctg atggtcaaga tatttttcgc aatcctgctt
     5821 tcctgccact gaatcagccc ccagcacccc aggaaaatgc cgcccgtgac gctgagcgcg
     5881 gtcagcgccg gaaacagtac ccgccctttg atcacctgcc agagcaggtt tttctgctgc
     5941 cgggacagtt cggcctctgt ggtgctgagc tgcgtgctca cggtggtgct cagcgtatcc
     6001 agttgctgtt tcacggatgt tgtatgttcg ctgatagcgt ctctgatttt ctgcctgttt
     6061 aagctcagtg ccttgtctac agactcttct agcttcctga actcgctgtt cagcatgttc
     6121 tcggtagaag cggctcgctc tttcagtttc ttctcgaagt ccgttcccat ttgtaaaaga
     6181 ttgctcatac agcgccccct ttagcctgag attccgcccc ccctccgggt cggcaatact
     6241 gatactgctt ctggttgtcc tcaccacctc aaaacctgcc gctgtgagcg cctcagtgac
     6301 atcctgacgc gtttttattg ctccggcacg gtaaagcgcc tcaagacccc gtgtaatcgc
     6361 ttctgcggcc tcctgtttcg cttttggcag attattcggt gtgacaagcg tccgtctgtt
     6421 ctccggtgcg ttcgggtcac gcagcccgta atgatgatta accagcgtct gccaggcatt
     6481 tatcctcggc ctgtccgcac ggtcgtagta cggctggagg cgcttaccgc tctgtagttc
     6541 catgttcgga atgacaaaat tcagttcgag ccgcccctta tcctggtgtt ccacccagag
     6601 aatgctgtac tggtcttttt cgagaccggg cataagaaca cgctcaaagc tcgtcatcac
     6661 tctttcgcgt tcacccggcg gtaactcctt ctccgcgaac gacagaacgc ctgacgtgta
     6721 tttcttcgaa aatggcgtgg catcaatgag ttcccggatt tcttccgggt taccccggag
     6781 caccgttgcg ccttcgcggt tacgctcccg ccccagcagg taatcgaccg ggccacttcc
     6841 accgcctttc cccctggcat gaaatttaac tatcatcccg tcccccctgt tccctgacag
     6901 cctgccgcaa ctggcgcaat tcaccctcga tggccatcag agcggccacc acatgaaccc
     6961 ggtcaatcga agaccagtgg ccgctgttca ccttccgggc tgtctggttc agattgttcc
     7021 cgatggcagc cagatgccgc agcaatggcg gcgatagtgt cggcagtttt cctgtgcgcg
     7081 caaccggctc ccccagacag acccgccgca tccattctgc cagtcgttta ccctcgcagc
     7141 gttccagtaa ccgggcgtgc tcctcatcgc taacccgtat cgtgagcatc ttctcgcgtt
     7201 tcatcggtat cattccccct aaaaaatccc ctattaagga gccttaagac catgaccaaa
     7261 caggaaaaaa cagcccttaa catggcccgc tttatcagaa gccagacgct gaccctgctg
     7321 gaaaaactca acgagctgga cgcggacgaa caggcagaca tctgcgaatc acttcacgac
     7381 cacgccgacg aactctaccg tagttgcctc gcacgcttcg gcgatgacgg tgaaagcaac
     7441 tgaaacatgc agctcccgga gacgttcaca gcttgcctgt gagcggatgc cgggagcaga
     7501 caagcccgtc agggcgcgtc agcgggtttt agcgggtgtc ggggcgcagc cctgacccag
     7561 tcacgtagcg atagcggagt gtatactggc ttaactatgt ggcatcagtg cggattataa
     7621 gaaaagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaatgccgca
     7681 tcaggcgctc ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc
     7741 gagcggtatc agctcactca aagacggtga tgcggttatc cacagaatca ggggataacg
     7801 ccaaaagaaa catgtgagca aaaggccagg aacaggaaga aggccgcgcc gctggcgttt
     7861 ttccataggc tccgcccccc tgacgagcat cacaaaaaca gatgctcaag tcagaggtgg
     7921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8041 gtggcgcttt ctcatagctc acgctgttgg tatctcggcc cggtgtaggt cgttcgctcc
     8101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8161 tatcgtcttg agtccaaccc ggaaagacac gtaaaaacgc cactggcagc agccattggt
     8221 aaccgtttag aggagttagt cttgaagtta tgcgccggtt aaggctaaac tgaaaggaca
     8281 gatttggtgg ctgtgctcct ccaagccagt taccttggtt caaagagttg ctgctctgag
     8341 aaccttcgaa aagccgcctt gctgggcggt tttttcgttt acggacaaga ctaccttcag
     8401 gatcgttaga tctcaagaca atc
//
