LOCUS       NZ_CP082071             7217 bp    DNA     linear   CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS20673 plasmid pMS20673D.
ACCESSION   NZ_CP082071
VERSION     NZ_CP082071.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934298
            Assembly: GCF_030515355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:30:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,072
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,737
            CDSs (with protein)               :: 4,737
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 70 of 215
            Pseudo Genes (incomplete)         :: 149 of 215
            Pseudo Genes (internal stop)      :: 44 of 215
            Pseudo Genes (multiple problems)  :: 45 of 215
            Pseudo Genes (short protein)      :: 4 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082071.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS20673"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS20673D"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Benita Westerlund-Wilkstrom"
                     /genotype="ST95"
     gene            <1..2160
                     /gene="mobQ"
                     /locus_tag="K6136_RS25330"
                     /old_locus_tag="K6136_25140"
     CDS             <1..2160
                     /gene="mobQ"
                     /locus_tag="K6136_RS25330"
                     /old_locus_tag="K6136_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2416..3108)
                     /locus_tag="K6136_RS25335"
                     /old_locus_tag="K6136_25145"
     CDS             complement(2416..3108)
                     /locus_tag="K6136_RS25335"
                     /old_locus_tag="K6136_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3640..3894)
                     /gene="mobC"
                     /locus_tag="K6136_RS25340"
                     /old_locus_tag="K6136_25150"
     CDS             complement(3640..3894)
                     /gene="mobC"
                     /locus_tag="K6136_RS25340"
                     /old_locus_tag="K6136_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            4073..5305
                     /gene="mobQ"
                     /locus_tag="K6136_RS25345"
                     /old_locus_tag="K6136_25155"
     CDS             4073..5305
                     /gene="mobQ"
                     /locus_tag="K6136_RS25345"
                     /old_locus_tag="K6136_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_302359606.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWAKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSATFEQLLTQVCQTWP
                     QYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTLMPVMSSVDRETRQL
                     QLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYAGYGVAVNHDA
                     DSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEWRREEDARTHT
                     TERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(5561..6253)
                     /locus_tag="K6136_RS25350"
                     /old_locus_tag="K6136_25160"
     CDS             complement(5561..6253)
                     /locus_tag="K6136_RS25350"
                     /old_locus_tag="K6136_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(6785..7039)
                     /gene="mobC"
                     /locus_tag="K6136_RS25355"
                     /old_locus_tag="K6136_25165"
     CDS             complement(6785..7039)
                     /gene="mobC"
                     /locus_tag="K6136_RS25355"
                     /old_locus_tag="K6136_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          2..359
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.05"
                     /coverage="100.0"
                     /evalue="1.4199999999999999e-176"
     Typing          complement(3896..4072)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          4073..4431
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(7041..7217)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          complement(2417..3108)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5562..6253)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2157
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.583"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4563..5302
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.571"
                     /coverage="100.0"
                     /evalue="6.6e-158"
ORIGIN
        1 atggcgattt atcatctcag catgaaaatt atctccagaa gcagcggcta cagcgccgta
       61 gcttccgctg cctatcgttc cggttcactg atgcttgatg agcgtaccgg actgactcac
      121 gactacaccc gcaaaagcgg tgtggctgaa gctgtgattc tgacccccgc gacagcccct
      181 gcctggtgta cgaaccgtgc cgaactctgg aatgccgttg agaaggccga atgccggaag
      241 aactcacaac ttgcgcggga aattgaactc gctatccccc gtgaactgcc gcaggatgcc
      301 gcacgggaaa ccgttctcgc ctttgtcaga gaaaactttg tcagtcaggg catgattgct
      361 gatgtggcgt tccatcacat ggataaaaca aatccccatg cgcacatcat gctgaccacg
      421 agggctgtcg gacctgctgg gttcggcgga aaggtcaggg actggaacga ccggacacat
      481 gccgagacgt ggcgtgcatc atgggctgac catgcaaaca gagcactggc gaacgccggt
      541 tatcaggaag agatagacca tcgttcatac gagcgacagg ggcttgagaa aacgcctggt
      601 attcacctcg gaaagtctgc ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc
      661 gaacagaatc gtctgattaa caggcttaac ctggaaatac agatttcccg cactgaactc
      721 aggaacagcg ggctgacaga gcctgcccgc cgtgtggctg accttctgaa tatcgtgctt
      781 cctgacaacg caacttcgta cacactacgt gacatcatcg aagcgttgcc gaaggacagt
      841 aatgcggcat ggaaactcac cagtaatgcc atgtcgatga tggcagatat gcaggcgacg
      901 cgcagacgct gggaagagct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc
      961 ctgaaaaaat catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggcg
     1021 gcaccggaat accgtagcga acagggaaaa atccagtcgc tgagccagca aatggaaaaa
     1081 ttgcgacagc attaccggaa ggtggagaaa caggacattc ccgcgagtca gacggcattt
     1141 gaaaaacagt ggcagcagtg gggggcatcc ggccttgcag ctctcagagt ccagttgctt
     1201 aaacgtgaag aggaattgcg ccggaaggag caggaggata cagaacgtag tcgcgcggag
     1261 cgacgagcgg aacagctcag gaaaaatgac aatgccgtta tggattcagg ggtactgagc
     1321 gcagtcgtta cggggtatgg cgaggccccc atgcctggta acggggaaat gacctgttac
     1381 cttctgcttc acaaccggaa cggagaatac acgctctggg ggaacgagct ggagaaatac
     1441 cgcaccagaa tctttgagcg tgttgattta atgcgtgaca ggagtggtta tatctgtgac
     1501 cgaagcgaaa tcggtcaaca tccaccactg caacgtgttt attcctctgc gacctttgag
     1561 caacttctga cgcaggtttg ccagacatgg ccgcaatata cccgcaatct ccgccagccg
     1621 aagacatggc cggagtcttt ctgtcttgga gaagaccgcc agccagccat gccatcactg
     1681 gcagcaagga aggtggattt tacgcaggga cgtctgctac cgacgctgat gccggtaatg
     1741 agttctgtcg accgggaaac ccgccagctt cagcttttgc tggtcatggg cgtggatgac
     1801 tctctgggcg gtgttgtccg gcttaatggt acgctgtatc ccgcgtttgc cgttccctca
     1861 gccgataaca gccagctggt catcagtgca ctgaccgata aggggttacg gtatgcaggg
     1921 tatggtgttg ccgtaaatca cgatgcagac agccacatat cacctgcgcc ggaacttatg
     1981 gagtttcatc tgaaaacccg tgaggcaccg ctttttgctg ccgtcaacac accagaaaaa
     2041 cagccggatc atctgttccg ctctctgggt ttcaatcgta cctgggatga atggcggcgc
     2101 gaggaagatg caagaacaca caccaccgag aggagacatg atcgcggttg gtcacagtga
     2161 atgtgaacaa tattttgttt tgtttaacat cgtgttatgt tcgtcgatgt tcttctatgt
     2221 tcatcgacag ggatgaatgc aagaagcaca cacataatgc aagcatagtt ttgtttttcc
     2281 gcccccaaaa attgggggtt cttttttcgc tgcattagag cgcttttgac gcaatttttc
     2341 acttcaatag taccttcgca ctactaacac aaaaaaaacg cagcagggag cgttttaggt
     2401 gccttaaaat cgattttaga tcgtcatctt gatctgtttg tcttccttga agcgaaaatg
     2461 cagggctaca accgtgcgcc cctttttgat cgtctcaacc gttacagcca ggtcgctttt
     2521 tttgttcagt tcgtcaacac agggttttat tagtatttta tttaagtctc tgaattgttt
     2581 gtatttattt tcaatcccta acattgaacg aaagtcatta agagcaataa ttctctctcc
     2641 cgtactgcgg aattgctgga ggaactcgta tattcttata ctgtaactac ttgataaacc
     2701 tgatatgttt tttataacaa cgcgcgtgaa ttgccccttc agttgcgtca gataaggcat
     2761 taccgcgtca gaaaaggtta ttcgagcctt ggcctcacct ttagcgtact gtgcccgata
     2821 ctggatccag cggaactcct cacgcttctc gtcattcttg agaatgatcg acctgtccca
     2881 taaacgatcg atcgcttcct gtaactgaac ctcggcattt ttccgcccca tgtcagggaa
     2941 tgaatcaaaa tattccgcag cggtaatggt cattgtcttt tgcaattcag catcagcacc
     3001 agatttcaat cgactgatac acagcaacag aaaacgttgt tcctgtaggg tcaacttgta
     3061 gctggcctca accaaagcat tcgacttgta ggcaatcaag tccgacatac aaagatcccg
     3121 ttcaaatgtg gtgatgcttt aatgtgcccc acaaataaga aatgtggaat attttttttt
     3181 atatcctcac atttctgtga ataacacctt aaattcctcc acctttacct taaattcctc
     3241 cacctaaaca ccttaaattc ctccaccttt accttaaatt cctccacata tacctctata
     3301 acatattgat tttattaatg aattttgacc ctaaaacaag tattaaaaac aagatcttaa
     3361 aaacaagatc taaaaactta acggcgagcc tgtggataac tcggggcgct gaaaacaaaa
     3421 aaccaataaa aacaatgtgt tgcaagaaag cagaattgca agttccgaat actccccttc
     3481 caaccctgtg gaaatccagt attcatgcgg gttcagcgca tatcacctac cgaaaaaccg
     3541 tgcgtatgtg ctcaacctgg cgacggattc acttccgctt cctcgcccac tcgctcgcta
     3601 acgctcggtc gctggggctg cggcgagcgg acgatacaat cactgtcctg gcagcgcctc
     3661 cagctcgtta atcagcggca gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt
     3721 caggaactgg agtaaccagg cacgcaactg cacctcctcc ggctttctga cctttgccag
     3781 caatgctgca cccacaacaa ccttctgcgc tgtgtccagg ctccggctct tcgccttcag
     3841 gcgctgtaat ctcgcctcag catcagcaat cttctgttcg agcgttctgc tcatgatgta
     3901 actccgtgcg cgatgaaaaa tcgcatttta gcgcgtcact ggtagtttaa aaactgagct
     3961 ggcataatgc acgacaaatc acaaagtgcg cacttataca atctccactc cgtttcgatt
     4021 gtgtgcgccc tgcggggctg aaagaaaact acaaaaaggc attacggcag aaatggcgat
     4081 ttatcatctc agcatgaaaa ttatctccag aagcagcggc tacagcgccg tagcttccgc
     4141 tgcctatcgt tccggttcac tgatgcttga tgagcgtacc ggactgactc acgactacac
     4201 ccgcaaaagc ggtgtggctg aagctgtgat tctgaccccc gcgacagccc ctgcctggtg
     4261 tacgaaccgt gccgaactct ggaatgccgt tgagaaggcc gaatgccgga agaactcaca
     4321 acttgcgcgg gaaattgaac tcgctatccc ccgtgaactg ccgcaggatg ccgcacggga
     4381 aaccgttctc gcctttgtca gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc
     4441 gttccatcac atggataaaa caaatcccca tgcgcacatc atgctgacca cgagggctgt
     4501 cggacctgct gggttcggcg gaaaggtcag ggactggaac gaccggacac atgccgagac
     4561 gtggcgtgca tcatgggcga aataccgcac cagaatcttt gagcgtgttg atttaatgcg
     4621 tgacaggagt ggttatatct gtgaccgaag cgaaatcggt caacatccac cactgcaacg
     4681 tgtttattcc tctgcgacct ttgagcaact tctgacgcag gtttgccaga catggccgca
     4741 atatacccgc aatctccgcc agccgaagac atggccggag tctttctgtc ttggagaaga
     4801 ccgccagcca gccatgccat cactggcagc aaggaaggtg gattttacgc agggacgtct
     4861 gctaccgacg ctgatgccgg taatgagttc tgtcgaccgg gaaacccgcc agcttcagct
     4921 tttgctggtc atgggcgtgg atgactctct gggcggtgtt gtccggctta atggtacgct
     4981 gtatcccgcg tttgccgttc cctcagccga taacagccag ctggtcatca gtgcactgac
     5041 cgataagggg ttacggtatg cagggtatgg tgttgccgta aatcacgatg cagacagcca
     5101 catatcacct gcgccggaac ttatggagtt tcatctgaaa acccgtgagg caccgctttt
     5161 tgctgccgtc aacacaccag aaaaacagcc ggatcatctg ttccgctctc tgggtttcaa
     5221 tcgtacctgg gatgaatggc ggcgcgagga agatgcaaga acacacacca ccgagaggag
     5281 acatgatcgc ggttggtcac agtgaatgtg aacaatattt tgttttgttt aacatcgtgt
     5341 tatgttcgtc gatgttcttc tatgttcatc gacagggatg aatgcaagaa gcacacacat
     5401 aatgcaagca tagttttgtt tttccgcccc caaaaattgg gggttctttt ttcgctgcat
     5461 tagagcgctt ttgacgcaat ttttcacttc aatagtacct tcgcactact aacacaaaaa
     5521 aaacgcagca gggagcgttt taggtgcctt aaaatcgatt ttagatcgtc atcttgatct
     5581 gtttgtcttc cttgaagcga aaatgcaggg ctacaaccgt gcgccccttt ttgatcgtct
     5641 caaccgttac agccaggtcg ctttttttgt tcagttcgtc aacacagggt tttattagta
     5701 ttttatttaa gtctctgaat tgtttgtatt tattttcaat ccctaacatt gaacgaaagt
     5761 cattaagagc aataattctc tctcccgtac tgcggaattg ctggaggaac tcgtatattc
     5821 ttatactgta actacttgat aaacctgata tgttttttat aacaacgcgc gtgaattgcc
     5881 ccttcagttg cgtcagataa ggcattaccg cgtcagaaaa ggttattcga gccttggcct
     5941 cacctttagc gtactgtgcc cgatactgga tccagcggaa ctcctcacgc ttctcgtcat
     6001 tcttgagaat gatcgacctg tcccataaac gatcgatcgc ttcctgtaac tgaacctcgg
     6061 catttttccg ccccatgtca gggaatgaat caaaatattc cgcagcggta atggtcattg
     6121 tcttttgcaa ttcagcatca gcaccagatt tcaatcgact gatacacagc aacagaaaac
     6181 gttgttcctg tagggtcaac ttgtagctgg cctcaaccaa agcattcgac ttgtaggcaa
     6241 tcaagtccga catacaaaga tcccgttcaa atgtggtgat gctttaatgt gccccacaaa
     6301 taagaaatgt ggaatatttt tttttatatc ctcacatttc tgtgaataac accttaaatt
     6361 cctccacctt taccttaaat tcctccacct aaacacctta aattcctcca cctttacctt
     6421 aaattcctcc acatatacct ctataacata ttgattttat taatgaattt tgaccctaaa
     6481 acaagtatta aaaacaagat cttaaaaaca agatctaaaa acttaacggc gagcctgtgg
     6541 ataactcggg gcgctgaaaa caaaaaacca ataaaaacaa tgtgttgcaa gaaagcagaa
     6601 ttgcaagttc cgaatactcc ccttccaacc ctgtggaaat ccagtattca tgcgggttca
     6661 gcgcatatca cctaccgaaa aaccgtgcgt atgtgctcaa cctggcgacg gattcacttc
     6721 cgcttcctcg cccactcgct cgctaacgct cggtcgctgg ggctgcggcg agcggacgat
     6781 acaatcactg tcctggcagc gcctccagct cgttaatcag cggcagtatg cgcgtcacat
     6841 cagcctgtcg tgtcacctct gccttcagga actggagtaa ccaggcacgc aactgcacct
     6901 cctccggctt tctgaccttt gccagcaatg ctgcacccac aacaaccttc tgcgctgtgt
     6961 ccaggctccg gctcttcgcc ttcaggcgct gtaatctcgc ctcagcatca gcaatcttct
     7021 gttcgagcgt tctgctcatg atgtaactcc gtgcgcgatg aaaaatcgca ttttagcgcg
     7081 tcactggtag tttaaaaact gagctggcat aatgcacgac aaatcacaaa gtgcgcactt
     7141 atacaatctc cactccgttt cgattgtgtg cgccctgcgg ggctgaaaga aaactacaaa
     7201 aaggcattac ggcagaa
//
