LOCUS       NZ_CP082070            10847 bp    DNA     linear   CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS20673 plasmid pMS20673C.
ACCESSION   NZ_CP082070
VERSION     NZ_CP082070.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934298
            Assembly: GCF_030515355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10847)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:30:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,072
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,737
            CDSs (with protein)               :: 4,737
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 70 of 215
            Pseudo Genes (incomplete)         :: 149 of 215
            Pseudo Genes (internal stop)      :: 44 of 215
            Pseudo Genes (multiple problems)  :: 45 of 215
            Pseudo Genes (short protein)      :: 4 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082070.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS20673"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS20673C"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Benita Westerlund-Wilkstrom"
                     /genotype="ST95"
     gene            1..195
                     /locus_tag="K6136_RS25255"
                     /old_locus_tag="K6136_25065"
     CDS             1..195
                     /locus_tag="K6136_RS25255"
                     /old_locus_tag="K6136_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1037..1174)
                     /locus_tag="K6136_RS25260"
                     /old_locus_tag="K6136_25070"
     CDS             complement(1037..1174)
                     /locus_tag="K6136_RS25260"
                     /old_locus_tag="K6136_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            1222..1563
                     /locus_tag="K6136_RS25265"
                     /old_locus_tag="K6136_25075"
     CDS             1222..1563
                     /locus_tag="K6136_RS25265"
                     /old_locus_tag="K6136_25075"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058755.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(1560..3125)
                     /locus_tag="K6136_RS25270"
                     /old_locus_tag="K6136_25080"
     CDS             complement(1560..3125)
                     /locus_tag="K6136_RS25270"
                     /old_locus_tag="K6136_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_023150412.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKSFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            3385..3822
                     /locus_tag="K6136_RS25275"
                     /old_locus_tag="K6136_25085"
     CDS             3385..3822
                     /locus_tag="K6136_RS25275"
                     /old_locus_tag="K6136_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            3903..4322
                     /locus_tag="K6136_RS25280"
                     /old_locus_tag="K6136_25090"
     CDS             3903..4322
                     /locus_tag="K6136_RS25280"
                     /old_locus_tag="K6136_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4547..4870)
                     /gene="mobA"
                     /locus_tag="K6136_RS25285"
                     /old_locus_tag="K6136_25095"
     CDS             complement(4547..4870)
                     /gene="mobA"
                     /locus_tag="K6136_RS25285"
                     /old_locus_tag="K6136_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            5154..5321
                     /locus_tag="K6136_RS25290"
                     /old_locus_tag="K6136_25100"
     CDS             5154..5321
                     /locus_tag="K6136_RS25290"
                     /old_locus_tag="K6136_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_302359603.1"
                     /translation="MNKRQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5693..5887
                     /locus_tag="K6136_RS25295"
                     /old_locus_tag="K6136_25105"
     CDS             5693..5887
                     /locus_tag="K6136_RS25295"
                     /old_locus_tag="K6136_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6729..6848)
                     /locus_tag="K6136_RS25300"
                     /old_locus_tag="K6136_25110"
     CDS             complement(6729..6848)
                     /locus_tag="K6136_RS25300"
                     /old_locus_tag="K6136_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_072145416.1"
                     /translation="MGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ"
                     /name="colicin release lysis protein"
     gene            7029..7253
                     /locus_tag="K6136_RS25305"
                     /old_locus_tag="K6136_25115"
     CDS             7029..7253
                     /locus_tag="K6136_RS25305"
                     /old_locus_tag="K6136_25115"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_302359604.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPRGK
                     IKPIYAIQSTLFGPSNPIWIARTVYINKE"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(7254..8819)
                     /locus_tag="K6136_RS25310"
                     /old_locus_tag="K6136_25120"
     CDS             complement(7254..8819)
                     /locus_tag="K6136_RS25310"
                     /old_locus_tag="K6136_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_023150412.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKSFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            9079..9516
                     /locus_tag="K6136_RS25315"
                     /old_locus_tag="K6136_25125"
     CDS             9079..9516
                     /locus_tag="K6136_RS25315"
                     /old_locus_tag="K6136_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            9597..10016
                     /locus_tag="K6136_RS25320"
                     /old_locus_tag="K6136_25130"
     CDS             9597..10016
                     /locus_tag="K6136_RS25320"
                     /old_locus_tag="K6136_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(10241..10564)
                     /gene="mobA"
                     /locus_tag="K6136_RS25325"
                     /old_locus_tag="K6136_25135"
     CDS             complement(10241..10564)
                     /gene="mobA"
                     /locus_tag="K6136_RS25325"
                     /old_locus_tag="K6136_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          complement(4880..5119)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(5418..5658)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.587"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          complement(10574..10813)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          225..754
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.59"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          5917..6446
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.227"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(4551..4870)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.15e-62"
     Typing          complement(10245..10564)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.15e-62"
ORIGIN
        1 atgaacaagc agcagcaagc cgtactcaac atggcaggat tcataaaaag ccaaagcctg
       61 acgctgctcg aaaagctgga cgcactcgat gctgacgaac aggctaccat gtgtgagaag
      121 ctgcacgaac tcgcagaaga actccagaac agcatccaga cacgctttga agtagaaaat
      181 agcacagaga gataacgctc aggacaggat gccgaacggc acaacagagc gtaaggagaa
      241 aatgtcacat aaggcgctct tccgcttcct cgctcactga ctcgctacgc tcggacgttc
      301 gactgcggcg agcggtgtca gctcactcaa aagcggtaat acggttatcc acagaatcag
      361 gggataaagc cggaaagaac atgtgagcaa aaagcaaagc accggaagaa gccagcgccg
      421 caggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagc
      481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      601 tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcggctc ggtgtaggtc
      661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttagcgcc actggcagca
      781 gccgttggta acttggttag cagagcgatg atggcgcaaa tggtgcaaca gagttcttga
      841 agtagtggcc cgactacggc tacactggaa ggacagtatt tggtatctgc gctctgctga
      901 agccagttac ctcggttaag cagttcccca actgacttaa ccttcgatca aaccgcctcc
      961 ccaggcggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     1021 gatcctttga tcttttctac tgaacagaga tccccgtcaa tttagaagaa gatgatggag
     1081 ccacagtccc tccctgaaca tcacgtatat agttagcctg acatccaaca aggaggttta
     1141 tagcgaatat tcccacaaaa aatcttttcc tcataactcg atccttataa aatgaaaaga
     1201 atatataacg aggtttaatt tatgagttta agatactaca taaaaaatat tttgtttggc
     1261 ctatactgcg cacttatata tatatacctt ataacaaaaa acaacgaagg gtattatttc
     1321 ctagcgtcag ataagatgct atacgcaata gtgataagca ctattctatg cccatattca
     1381 aaatatgcta ttgaacacat attttttaag ttcataaaga aagatttttt cagaaaaaga
     1441 aaaaacctaa ataatgcccc cgtggcaaaa ttaaacctat ttatgctata caatctactt
     1501 tgtttggtcc tagcaatccc atttggattg ctaggactgt ttatatcaat aaagaataat
     1561 taaataccca acacctcatt tatagtatta agtttattct tatcaataaa ggcacataga
     1621 atgcctgtaa caatagcaat cccccagata cctaatgtag ttccagcaag cacactaaaa
     1681 agtaaaacaa caacataact aactccagca tctacagctt tcttctctaa tgtaagaaat
     1741 agcggcttcc agtcacctgt atccttaatt tttaggatat cagataccac atcatatcca
     1801 aaagaaacat gtcccgtaat cttcaagtac ttggcaaact gatctaaatg cttagcccag
     1861 tcttcatact taaccgcctc cagtgcattg aaaattgcat cacggtctgc tttgctgaat
     1921 ttcttattta atacatcctt gtatttctca aaagcagcta gagcttcatt cacattgctg
     1981 attttcttac ctttagattt atcagcaagt tcctgtgcca tttttgaata tttttcacca
     2041 tatttttcag atagcgtttg ataaaagcta actgttgcat ccacagcatc cttaatctgc
     2101 gagttcagga gattagtctg tgctgttttc aaattatctt cagcatcatg aatacgtgaa
     2161 actgctgtac ttcgcttatt attagcctga gaaatagcct cttggattcc atttatctca
     2221 gagctaatct ggttaagacg cgtttctgat gctgttacct gcttttgttt ttcctccatc
     2281 tcatcaccag ctctcgcccg tagtctggtg gcctcaaaaa aaggacgact ctgaagcgga
     2341 tcattcgccc taggagataa aagtttaacc ctttcatcca gttctttata tttagcagat
     2401 gcctgatcca actcatttcg ttttccggac agcgtattcg tttctgcatc acgggcatga
     2461 atactggagc ttaaacgggt attgagcgta ttaatctctt catccacttt cgccagctca
     2521 gattgtgcag cagcaagatt tttttgtgcc acctccacag cccgggcctc ttcactcaat
     2581 gctgccaggc gtttctcttc atcctcagcc agtttcaatt ggcgttctgt ttcagcctgc
     2641 tccttctcta tctctttacg tcgttgttct gcttcctgaa aagacttttc cgcagcttcc
     2701 gcttctttac gggctttttc ttctgctttt gcaaggcgca accgctctgc ttctgcctgc
     2761 atcgctgcat tattggcatg agccaggtca ataacctccg gatgagtgga attatggcga
     2821 agcgcctcat tcacaatatc cttcagatgt tgagtcagcg catcccggtt tgcttttgct
     2881 ttagcctgtg cttctgcggc agcttttgct cgggcagcct gttctgcctg cgttttcttc
     2941 aattgagcag tagaccattt agctgtggca tgaatggctg cagaactttc acttttgcta
     3001 cctccagttc caccaccacc gccagagcca ctcccgtctg gattaccatt caaaagagta
     3061 atgattacct gccccttatc atcataagga acaccatctt tatagtacgc tacagctgtt
     3121 tccattataa aatcctcttt gacattaaaa acaaatagtt aaaagcaata tactgtatat
     3181 ataatcactg gttttatata cagtttaaaa ttacgccgct gcattttccc tgtcaaccct
     3241 gtggattttc atttttgtga aaacgatcaa aaaaacagcc acccacacca cgtgctattt
     3301 ccacccgatg ccacaaaaac cagcacaaac attaccgttc tcagacctca ttatgtttta
     3361 ctgaaaccat gagatgagac acctatggga cactgtaact ttatggcatg gcacacactc
     3421 cgggacgcac tgaaaatgac tggaagatcg cgttcacagt tttaccgtga tatgcgctca
     3481 ggtcttgtca gttaccgtac cagccaggac ggacgacggg agtttgaaac cagtgaactg
     3541 atccgggcat acggcgaatt aaagcggaat gagacaccag aaaggcacac tcaaggacac
     3601 tcagaaaaac ctcatgatca gcagacagaa cgcattctcc gggaactgaa tgagctgaaa
     3661 caatgcctga cgctgatgct tgaggataaa caagcccagg atacggagcg ccggcgccag
     3721 gaagcagaac ggaaacagct acagaatgag atagcccagc tcaggcaggc tctggagcag
     3781 gaaaagaaac ggggattctg gtccaggctg ttcggtcact gaacgcgctc agaaactgat
     3841 gataagataa tcttcggata ataactcacc gagaataaat actttaaggt agggagacac
     3901 tcatgagacg taccggaaac aaactttgtc ttatcgccat gataacagca acagtagctc
     3961 tcacagcctg taccccaaag ggcagcgtgg aacaacatac ccggcattac gtatatgctt
     4021 ctgatgacgg ttttgatccc aacttttcca cccaaaaagc cgacacaaca cgaatgatgg
     4081 tgcctttttt tcggcagttc tgggatatgg gagctaaaga taaagcgaca ggaaaatcac
     4141 ggagtgatgt gcaacaacgc attcaacagt ttcacagcca ggaattttta aactcactcc
     4201 ggggcacaac tcaatttgcg ggtactgatt accgcagcaa agaccttacc ccgaaaaaat
     4261 ccaggctgct ggctgacacg atttctgcgg tttatctcga tggctacgag ggcagacagt
     4321 aagtggattt accataatcc cttaattgta cgcaccgctg aaacgcgctg aacgcaattc
     4381 tgacgcaaat accgcaaaaa gagaggttta gccccgaaaa aacaggaagg atgcgtcagg
     4441 atcgttttca gctcactgca tagctatgca tgaaagcgaa tggtgatcac tttgggagct
     4501 tacggtgttc ataccgtctg ttttcgacag ttttctctcc gggaagctaa tctgccataa
     4561 gcctggataa cagggcacgg tgatactccg taatagcgat cagcacctcc gcatactccc
     4621 tgtccccgac acgcttgccg tcgataaaga gttttttctg taacgccccc agtctcataa
     4681 cttctttcag tacccgatca tcagtcagtg agttaacttt cttaccgaga gctgccgccc
     4741 gtaaaaaacc ggaaacggtc ttgccgcatt cagcagccct ttttctgatt tcctggtctt
     4801 cttctgccgt cagacgaaca gggcgactga tagcccgcct gcgcgtatta cttccacttc
     4861 gttttgtcat aaaacatggc tccgtatctg acatgggtgt cggggcaaag ccctgaccag
     4921 ggtaattgta atagcgtgca tgtatgcgcg gtataacaat tacacatcct gtcctgtcag
     4981 caagttcgaa ttaccgacaa aggcacggca gttttttggc agtgacagta ttgtcaggca
     5041 gaaatacccg tcaaagcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt
     5101 acgcgatata atcacacaac gcagatgaca gacagaacgg agaaaacgaa aagatgaaca
     5161 agcggcagca agccgtactc aacatggcag gattcataaa aagccaaagc ctgacgctgc
     5221 tcgaaaagct ggacgcactc gatgctgacg aacaggctac catgtgtgag aagctgcacg
     5281 aactcgcaga agaactccag aacagcatcc agacacgctg aagtagttcc tggtcttctt
     5341 ctgccgtcag acccaaatgg gcgactgata gcctgcctgc gcgtattact tccacttcgt
     5401 tttgtcataa aacatggctc cgtatctgac atgggtgtcg ggggcaaagc cctgaccagg
     5461 gtaattgtaa tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc
     5521 aagttcgaat taccgacaaa ggcacggcag ttttttggca gtgacagtat tgtcaggcag
     5581 aaatacccgt caaagcgtca gacggcagaa taccggcggt aaaacagtcg aaaagtggta
     5641 cgcgatataa tcacacaacg cagatgacag acagaacgga gaaaacgaaa agatgaacaa
     5701 gcagcagcaa gccgtactca acatggcagg attcataaaa agccaaagcc tgacgctgct
     5761 cgaaaagctg gacgcactcg atgctgacga acaggctacc atgtgtgaga agctgcacga
     5821 actcgcagaa gaactccaga acagcatcca gacacgcttt gaagtagaaa atagcacaga
     5881 gagataacgc tcaggacagg atgccgaacg gcacaacaga gcgtaaggag aaaatgtcac
     5941 ataaggcgct cttccgcttc ctcgctcact gactcgctac gctcggacgt tcgactgcgg
     6001 cgagcggtgt cagctcactc aaaagcggta atacggttat ccacagaatc aggggataaa
     6061 gccggaaaga acatgtgagc aaaaagcaaa gcaccggaag aagccagcgc cgcaggcgtt
     6121 tttccatagg ctccgccccc cctgacgagc atcacaaaaa tcgacgctca agccagaggt
     6181 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cctggaagct ccctcgtgcg
     6241 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6301 cgtggcgctt tctcatagct cacgctgttg gtatctcggc tcggtgtagg tcgttcgctc
     6361 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6421 ctatcgtctt gagtccaacc cggtaagaca cgacttagcg ccactggcag cagccgttgg
     6481 taacttggtt agcagagcga tgatggcgca aatggtgcaa cagagttctt gaagtagtgg
     6541 cccgactacg gctacactgg aaggacagta tttggtatct gcgctctgct gaagccagtt
     6601 acctcggtta agcagttccc caactgactt aaccttcgat caaaccgcct ccccaggcgg
     6661 ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6721 gatcttttct actgaacaga gatccccgtc aatttagaag aagatgatgg agccacagtc
     6781 cctccctgaa catcacgtat atagttagcc tgacatccaa caaggaggtt tatagcgaat
     6841 attcccacta aaaaatcttt tcctcataac tcgatcctta taaaatgaaa agaatatata
     6901 acgaggttta atttatgagt ttaagatact acataaaaaa tattttgttt ggcctatact
     6961 gcgcacttat atatatatac cttataacaa aaaacaacga agggtattat ttcctagcgt
     7021 cagataagat gctatacgca atagtgataa gcactattct atgcccatat tcaaaatatg
     7081 ctattgaaca catatttttt aagttcataa agaaagattt tttcagaaaa agaaaaaacc
     7141 taaataatgc cccccgtggc aaaattaaac ctatttatgc tatacaatct actttgtttg
     7201 gtcctagcaa tcccatttgg attgctagga ctgtttatat caataaagaa taattaaata
     7261 cccaacacct catttatagt attaagttta ttcttatcaa taaaggcaca tagaatgcct
     7321 gtaacaatag caatccccca gatacctaat gtagttccag caagcacact aaaaagtaaa
     7381 acaacaacat aactaactcc agcatctaca gctttcttct ctaatgtaag aaatagcggc
     7441 ttccagtcac ctgtatcctt aatttttagg atatcagata ccacatcata tccaaaagaa
     7501 acatgtcccg taatcttcaa gtacttggca aactgatcta aatgcttagc ccagtcttca
     7561 tacttaaccg cctccagtgc attgaaaatt gcatcacggt ctgctttgct gaatttctta
     7621 tttaatacat ccttgtattt ctcaaaagca gctagagctt cattcacatt gctgattttc
     7681 ttacctttag atttatcagc aagttcctgt gccatttttg aatatttttc accatatttt
     7741 tcagatagcg tttgataaaa gctaactgtt gcatccacag catccttaat ctgcgagttc
     7801 aggagattag tctgtgctgt tttcaaatta tcttcagcat catgaatacg tgaaactgct
     7861 gtacttcgct tattattagc ctgagaaata gcctcttgga ttccatttat ctcagagcta
     7921 atctggttaa gacgcgtttc tgatgctgtt acctgctttt gtttttcctc catctcatca
     7981 ccagctctcg cccgtagtct ggtggcctca aaaaaaggac gactctgaag cggatcattc
     8041 gccctaggag ataaaagttt aaccctttca tccagttctt tatatttagc agatgcctga
     8101 tccaactcat ttcgttttcc ggacagcgta ttcgtttctg catcacgggc atgaatactg
     8161 gagcttaaac gggtattgag cgtattaatc tcttcatcca ctttcgccag ctcagattgt
     8221 gcagcagcaa gatttttttg tgccacctcc acagcccggg cctcttcact caatgctgcc
     8281 aggcgtttct cttcatcctc agccagtttc aattggcgtt ctgtttcagc ctgctccttc
     8341 tctatctctt tacgtcgttg ttctgcttcc tgaaaagact tttccgcagc ttccgcttct
     8401 ttacgggctt tttcttctgc ttttgcaagg cgcaaccgct ctgcttctgc ctgcatcgct
     8461 gcattattgg catgagccag gtcaataacc tccggatgag tggaattatg gcgaagcgcc
     8521 tcattcacaa tatccttcag atgttgagtc agcgcatccc ggtttgcttt tgctttagcc
     8581 tgtgcttctg cggcagcttt tgctcgggca gcctgttctg cctgcgtttt cttcaattga
     8641 gcagtagacc atttagctgt ggcatgaatg gctgcagaac tttcactttt gctacctcca
     8701 gttccaccac caccgccaga gccactcccg tctggattac cattcaaaag agtaatgatt
     8761 acctgcccct tatcatcata aggaacacca tctttatagt acgctacagc tgtttccatt
     8821 ataaaatcct ctttgacatt aaaaacaaat agttaaaagc aatatactgt atatataatc
     8881 actggtttta tatacagttt aaaattacgc cgctgcattt tccctgtcaa ccctgtggat
     8941 tttcattttt gtgaaaacga tcaaaaaaac agccacccac accacgtgct atttccaccc
     9001 gatgccacaa aaaccagcac aaacattacc gttctcagac ctcattatgt tttactgaaa
     9061 ccatgagatg agacacctat gggacactgt aactttatgg catggcacac actccgggac
     9121 gcactgaaaa tgactggaag atcgcgttca cagttttacc gtgatatgcg ctcaggtctt
     9181 gtcagttacc gtaccagcca ggacggacga cgggagtttg aaaccagtga actgatccgg
     9241 gcatacggcg aattaaagcg gaatgagaca ccagaaaggc acactcaagg acactcagaa
     9301 aaacctcatg atcagcagac agaacgcatt ctccgggaac tgaatgagct gaaacaatgc
     9361 ctgacgctga tgcttgagga taaacaagcc caggatacgg agcgccggcg ccaggaagca
     9421 gaacggaaac agctacagaa tgagatagcc cagctcaggc aggctctgga gcaggaaaag
     9481 aaacggggat tctggtccag gctgttcggt cactgaacgc gctcagaaac tgatgataag
     9541 ataatcttcg gataataact caccgagaat aaatacttta aggtagggag acactcatga
     9601 gacgtaccgg aaacaaactt tgtcttatcg ccatgataac agcaacagta gctctcacag
     9661 cctgtacccc aaagggcagc gtggaacaac atacccggca ttacgtatat gcttctgatg
     9721 acggttttga tcccaacttt tccacccaaa aagccgacac aacacgaatg atggtgcctt
     9781 tttttcggca gttctgggat atgggagcta aagataaagc gacaggaaaa tcacggagtg
     9841 atgtgcaaca acgcattcaa cagtttcaca gccaggaatt tttaaactca ctccggggca
     9901 caactcaatt tgcgggtact gattaccgca gcaaagacct taccccgaaa aaatccaggc
     9961 tgctggctga cacgatttct gcggtttatc tcgatggcta cgagggcaga cagtaagtgg
    10021 atttaccata atcccttaat tgtacgcacc gctgaaacgc gctgaacgca attctgacgc
    10081 aaataccgca aaaagagagg tttagccccg aaaaaacagg aaggatgcgt caggatcgtt
    10141 ttcagctcac tgcatagcta tgcatgaaag cgaatggtga tcactttggg agcttacggt
    10201 gttcataccg tctgttttcg acagttttct ctccgggaag ctaatctgcc ataagcctgg
    10261 ataacagggc acggtgatac tccgtaatag cgatcagcac ctccgcatac tccctgtccc
    10321 cgacacgctt gccgtcgata aagagttttt tctgtaacgc ccccagtctc ataacttctt
    10381 tcagtacccg atcatcagtc agtgagttaa ctttcttacc gagagctgcc gcccgtaaaa
    10441 aaccggaaac ggtcttgccg cattcagcag ccctttttct gatttcctgg tcttcttctg
    10501 ccgtcagacg aacagggcga ctgatagccc gcctgcgcgt attacttcca cttcgttttg
    10561 tcataaaaca tggctccgta tctgacatgg gtgtcggggc aaagccctga ccagggtaat
    10621 tgtaatagcg tgcatgtatg cgcggtataa caattacaca tcctgtcctg tcagcaagtt
    10681 cgaattaccg acaaaggcac ggcagttttt tggcagtgac agtattgtca ggcagaaata
    10741 cccgtcaaag cgtcagacgg cagaataccg gcggtaaaac agtcgaaaag tggtacgcga
    10801 tataatcaca caacgcagat gacagacaga acggagaaaa cgaaaag
//
