LOCUS       NZ_CP082065             2183 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS20674 plasmid pMS20674C, complete
            sequence.
ACCESSION   NZ_CP082065
VERSION     NZ_CP082065.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934299
            Assembly: GCF_030515255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 171x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:16:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,017
            CDSs (total)                      :: 4,899
            Genes (coding)                    :: 4,679
            CDSs (with protein)               :: 4,679
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 84 of 220
            Pseudo Genes (incomplete)         :: 151 of 220
            Pseudo Genes (internal stop)      :: 38 of 220
            Pseudo Genes (multiple problems)  :: 49 of 220
            Pseudo Genes (short protein)      :: 4 of 220
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082065.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS20674"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS20674C"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Adam Irwin"
                     /genotype="ST1193"
     gene            1..267
                     /locus_tag="K6130_RS25075"
                     /old_locus_tag="K6130_24875"
     CDS             1..267
                     /locus_tag="K6130_RS25075"
                     /old_locus_tag="K6130_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            1215..2180
                     /locus_tag="K6130_RS25080"
                     /old_locus_tag="K6130_24880"
     CDS             1215..2180
                     /locus_tag="K6130_RS25080"
                     /old_locus_tag="K6130_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          1609..1840
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 atgaatatta atattgaata cctgaatgga aataagacta ttggtttatt ttttttaaga
       61 agtgaagcgg tgattcctga caggtttaaa aaccttattt tgcttattga tggattaagt
      121 tttggcacat ttggttttca tccgcacgaa ggttttgagg atgaattaat tttatatatt
      181 cagaaaacaa acgagagggt aaaaactctt tttgtgaaaa tagatttaaa cggtatcaag
      241 tttggatttt taagaacgca ttcttagttc tggaaaagag ccagcggcag gctgaggtga
      301 taggtacgag attgcatgca atctctagtg ctctgtctat cctgcattat cctcagcatt
      361 atcctcagca ttatcctcag cattatcctc agccttgcca actcgacacc aatgcaggat
      421 agacaatccg atgtcaaatg ttaacactct gcgagtggta cattttcccc ggattatcgt
      481 cctgagcctg ccgctggctc tctttctacc gcctcgcttt gctcgttgct caacgcctca
      541 cagacacgga ttaaaatccg catccgttca ccgtttttta aagtccgtta aaagcatgat
      601 gccatctccg agagttaatc tcgtcaaatg ctaaatcgtg ggggtcccct ttggggttcc
      661 gatttagtga ttgacgacac caccgattaa aaaacttatg cggggtggat ggtttcacga
      721 agtgaggcca tccacctgta agacagggtt ttgtttttat tccctgtttt ggtgatcggg
      781 tgtgtggaaa aggttggggt aagccgttcg ggggtgcttg ttttgggggg ttaaaattgt
      841 ggttattttt tgcgcaattc tcgcgcgtga tccttgtatt tatacttaag ggataaatgg
      901 cggatatgaa atagtggttt agcccagtaa tgacgaggct ttgagtgggt tttgacaggt
      961 caaagaaaat ggagcagaat tgaggcgttt ttaatcggcg ttggggagtg cgtcaacact
     1021 ccccaacatt tcgaatgtgt cacctcagcg gcaaactctg gtgacatgta ctggctcgca
     1081 atgcacaggt acgtgatgaa tataccacat caaatcacag cctgcccaga tcggagcagg
     1141 cttaatgtca gaagataaat tcctttcgga ctacagccct caaatcacag cctgcccaga
     1201 tcggagcagg cttaatgtca gaagataaat tcctttcgga ctacagcccc cgtgatgcag
     1261 tttgggatac ccagcgcacg cttaccgatt ctgtcggggg tatctaccag actgctgctg
     1321 aattcgagcg ctatgcactc cgtatggcct cctgtagcgg tttgttacgt tttggttggt
     1381 ctaccatcat ggaaaccgga gaaacgcgcc tacggcttcg tagtgcgcaa ttttgccgtg
     1441 tccgtcattg ccctgtctgc cagtggagaa gaaccctcat gtggcaagcc cgtttttatc
     1501 aggctctacc gaaaatcgtt gtggattacc cgtcttcccg atggttgttt ctgacgttaa
     1561 ctgtcaggaa ctgcgagata ggtgaacttg gaacagtcct tacagcaatg aatgcggcgt
     1621 ttaagcgaat ggaaaagcga aaggagctat cacctgttca ggggtggatc agggctacgg
     1681 aggtgacgcg aggtaaggat ggcagcgcac atccgcattt tcactgtctg ctgatggtgc
     1741 aaccttcttg gtttaaaggg aagaactacg ttaagcacga acgttgggta gaactctggc
     1801 gcgattgctt gcgggtgaac tatgagccga atatcgatat tcgggcagta aaaactaaga
     1861 caggtgaggt tgtggccaac gttgccgagc aactgcaaag cgcggttgct gaaacgctga
     1921 aatactccgt taaaccggaa gatatggcaa acgatcctga gtggtttctt gagctgacgc
     1981 ggcagcttca caagcgccgt tttatctcga ccggtggggc gctaaaaaac gtcctccagt
     2041 tggatcgaga aaccaatgag gatcttgtca ttgccgacga tgtaggggat ggcactgatg
     2101 acgggaagcg gacggcgttt gtctgggatt caggtaaacg gcgttacaaa cgcgcccctg
     2161 agaaggataa atcggattaa cgt
//
