LOCUS       NZ_CP082064            17997 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS20674 plasmid pMS20674B, complete
            sequence.
ACCESSION   NZ_CP082064
VERSION     NZ_CP082064.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934299
            Assembly: GCF_030515255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17997)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17997)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 171x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:16:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,017
            CDSs (total)                      :: 4,899
            Genes (coding)                    :: 4,679
            CDSs (with protein)               :: 4,679
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 84 of 220
            Pseudo Genes (incomplete)         :: 151 of 220
            Pseudo Genes (internal stop)      :: 38 of 220
            Pseudo Genes (multiple problems)  :: 49 of 220
            Pseudo Genes (short protein)      :: 4 of 220
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082064.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..17997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS20674"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS20674B"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Adam Irwin"
                     /genotype="ST1193"
     gene            25..348
                     /locus_tag="K6130_RS24920"
                     /old_locus_tag="K6130_24725"
     CDS             25..348
                     /locus_tag="K6130_RS24920"
                     /old_locus_tag="K6130_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            557..>994
                     /locus_tag="K6130_RS24925"
                     /pseudo=""
     CDS             557..>994
                     /locus_tag="K6130_RS24925"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1026..1586
                     /locus_tag="K6130_RS24930"
                     /old_locus_tag="K6130_24735"
     CDS             1026..1586
                     /locus_tag="K6130_RS24930"
                     /old_locus_tag="K6130_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1593..1826
                     /locus_tag="K6130_RS24935"
                     /old_locus_tag="K6130_24740"
     CDS             1593..1826
                     /locus_tag="K6130_RS24935"
                     /old_locus_tag="K6130_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2310..2732)
                     /locus_tag="K6130_RS24940"
                     /old_locus_tag="K6130_24745"
     CDS             complement(2310..2732)
                     /locus_tag="K6130_RS24940"
                     /old_locus_tag="K6130_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2854..3132
                     /locus_tag="K6130_RS24945"
                     /old_locus_tag="K6130_24750"
     CDS             2854..3132
                     /locus_tag="K6130_RS24945"
                     /old_locus_tag="K6130_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3124..3414)
                     /locus_tag="K6130_RS24950"
                     /old_locus_tag="K6130_24755"
     CDS             complement(3124..3414)
                     /locus_tag="K6130_RS24950"
                     /old_locus_tag="K6130_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            3654..5300
                     /gene="cka"
                     /locus_tag="K6130_RS24955"
                     /old_locus_tag="K6130_24760"
     CDS             3654..5300
                     /gene="cka"
                     /locus_tag="K6130_RS24955"
                     /old_locus_tag="K6130_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(5297..5587)
                     /gene="cki"
                     /locus_tag="K6130_RS24960"
                     /old_locus_tag="K6130_24765"
     CDS             complement(5297..5587)
                     /gene="cki"
                     /locus_tag="K6130_RS24960"
                     /old_locus_tag="K6130_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            5617..5748
                     /locus_tag="K6130_RS24965"
                     /old_locus_tag="K6130_24770"
     CDS             5617..5748
                     /locus_tag="K6130_RS24965"
                     /old_locus_tag="K6130_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5943..6086)
                     /locus_tag="K6130_RS24970"
                     /old_locus_tag="K6130_24775"
     CDS             complement(5943..6086)
                     /locus_tag="K6130_RS24970"
                     /old_locus_tag="K6130_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(6754..6945)
                     /locus_tag="K6130_RS24975"
                     /old_locus_tag="K6130_24780"
     CDS             complement(6754..6945)
                     /locus_tag="K6130_RS24975"
                     /old_locus_tag="K6130_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7013..7336
                     /locus_tag="K6130_RS24980"
                     /old_locus_tag="K6130_24785"
     CDS             7013..7336
                     /locus_tag="K6130_RS24980"
                     /old_locus_tag="K6130_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7545..>7982
                     /locus_tag="K6130_RS24985"
                     /pseudo=""
     CDS             7545..>7982
                     /locus_tag="K6130_RS24985"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8014..8517
                     /locus_tag="K6130_RS24990"
     CDS             8014..8517
                     /locus_tag="K6130_RS24990"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_302344386.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLPGKNMGCDVSRGQEREISGV
                     AVRG"
                     /name="MbeB family mobilization protein"
     gene            8583..8816
                     /locus_tag="K6130_RS24995"
                     /old_locus_tag="K6130_24795"
     CDS             8583..8816
                     /locus_tag="K6130_RS24995"
                     /old_locus_tag="K6130_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(9321..9611)
                     /gene="cki"
                     /locus_tag="K6130_RS25000"
                     /old_locus_tag="K6130_24800"
     CDS             complement(9321..9611)
                     /gene="cki"
                     /locus_tag="K6130_RS25000"
                     /old_locus_tag="K6130_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_302344381.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTPKRN"
                     /name="cki"
     gene            9641..9772
                     /locus_tag="K6130_RS25005"
                     /old_locus_tag="K6130_24805"
     CDS             9641..9772
                     /locus_tag="K6130_RS25005"
                     /old_locus_tag="K6130_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(9966..10109)
                     /locus_tag="K6130_RS25010"
                     /old_locus_tag="K6130_24810"
     CDS             complement(9966..10109)
                     /locus_tag="K6130_RS25010"
                     /old_locus_tag="K6130_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(10777..10968)
                     /locus_tag="K6130_RS25015"
                     /old_locus_tag="K6130_24815"
     CDS             complement(10777..10968)
                     /locus_tag="K6130_RS25015"
                     /old_locus_tag="K6130_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            11036..11359
                     /locus_tag="K6130_RS25020"
                     /old_locus_tag="K6130_24820"
     CDS             11036..11359
                     /locus_tag="K6130_RS25020"
                     /old_locus_tag="K6130_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            11568..>12005
                     /locus_tag="K6130_RS25025"
                     /pseudo=""
     CDS             11568..>12005
                     /locus_tag="K6130_RS25025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            12037..12597
                     /locus_tag="K6130_RS25030"
                     /old_locus_tag="K6130_24830"
     CDS             12037..12597
                     /locus_tag="K6130_RS25030"
                     /old_locus_tag="K6130_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            12604..12837
                     /locus_tag="K6130_RS25035"
                     /old_locus_tag="K6130_24835"
     CDS             12604..12837
                     /locus_tag="K6130_RS25035"
                     /old_locus_tag="K6130_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(13321..13743)
                     /locus_tag="K6130_RS25040"
                     /old_locus_tag="K6130_24840"
     CDS             complement(13321..13743)
                     /locus_tag="K6130_RS25040"
                     /old_locus_tag="K6130_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(14134..14424)
                     /locus_tag="K6130_RS25045"
                     /old_locus_tag="K6130_24845"
     CDS             complement(14134..14424)
                     /locus_tag="K6130_RS25045"
                     /old_locus_tag="K6130_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            14664..16310
                     /gene="cka"
                     /locus_tag="K6130_RS25050"
                     /old_locus_tag="K6130_24850"
     CDS             14664..16310
                     /gene="cka"
                     /locus_tag="K6130_RS25050"
                     /old_locus_tag="K6130_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(16307..16597)
                     /gene="cki"
                     /locus_tag="K6130_RS25055"
                     /old_locus_tag="K6130_24855"
     CDS             complement(16307..16597)
                     /gene="cki"
                     /locus_tag="K6130_RS25055"
                     /old_locus_tag="K6130_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            16627..16758
                     /locus_tag="K6130_RS25060"
                     /old_locus_tag="K6130_24860"
     CDS             16627..16758
                     /locus_tag="K6130_RS25060"
                     /old_locus_tag="K6130_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(16952..17095)
                     /locus_tag="K6130_RS25065"
                     /old_locus_tag="K6130_24865"
     CDS             complement(16952..17095)
                     /locus_tag="K6130_RS25065"
                     /old_locus_tag="K6130_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(17763..17954)
                     /locus_tag="K6130_RS25070"
                     /old_locus_tag="K6130_24870"
     CDS             complement(17763..17954)
                     /locus_tag="K6130_RS25070"
                     /old_locus_tag="K6130_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          6582..6669
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          10605..10692
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          17591..17678
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(6015..6571)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10038..10594)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(17024..17580)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          339..1933
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7327..8462
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.945"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11350..12944
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          17761..17963
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="2.01e-13"
ORIGIN
        1 atgataccgg tgaaacgcga aaggatgctc acgatacggg tcacggatga ggaacatgcg
       61 cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat ggatgcgtaa ggtctgcctg
      121 ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg caccgccgct gttgcgccag
      181 ttcgcttcgg tcggcaataa cctgaatcag atagcccgga agataaacag cggacagtgg
      241 tcggggcatg accgggttca tgtggtggct gcactgatgg ccatcggacg tgaactgagt
      301 gagctgaggg atgaggtccg taaacaggga gaacgggatg atagttaagt ttcatgccag
      361 gggaaaaggc ggtggcagtg gtccggttga ttacctgctg ggccgggagc gtaaccggga
      421 aggggcgaga gttctgcggg gtgctcccga agaggtgcgg gaactcatcg atgccacgcc
      481 gtttgcgaaa aaatacacct cgggggttct gtcttttgca gagcagaccc tgccaccggg
      541 agagcgtgaa agggtgatgg agagctttga gcgggtactg atgcccggtc ttgaaaaaaa
      601 tcagtacagc atcctgtggg tggagcacca ggacaagggg cgtcttgagc tgaattttgt
      661 catcccgaat atggagctgg cgagcggaaa acggctccag ccctattacg accgagcaga
      721 ccgtccgcga atcaatgcct ggcagacact ggtgaatcac cattacgggc tgcacgaccc
      781 gaacgcaccg gagaacaggc ggacacttgt caccccgaat aatctgccga aagcgaaaca
      841 ggaggccgca gaagcgatta cgaggggact ggaggctctt taccatgccg gagcgctaaa
      901 aacgcgtcag gatgtcactg aggcgcttac agcggcaggt tttgaggttg tgaggacaac
      961 caggagcagt atcagtatcg ccgacccgga gggtgggcgt aatctcaggc tgaaaggggc
     1021 gctgtatgag caatctttta caaatgggga cagacttcga gaagaaactg aaagagcgtg
     1081 ccgtctctac agagaacatg ctgaacagcg agttcaggaa gctcgaagcg tctgtagaca
     1141 gggaactgaa cttaaacggg cagaaaatca gagacgctat cagcgcacac acagcagcgg
     1201 tgaaagaaca actggagacg ctgaacagtg ccgtgaacgc gcagttcagc gccacagaga
     1261 acgaactggc ccggcggcag gaagagctgc tctggaagct ggtcaaaggg cggatactgt
     1321 acccgtcgct gacggcgctt tgcgtcacgg gcgggatttt tctggcgtca tgggggctga
     1381 ttcagtggca ggaaagccgg attgcggcga acatcctggc tatcagggac caggaagaga
     1441 cgctggcgaa actccgggaa aaaacatggg gtgtgacgta tcacgagggc aggaacggga
     1501 aatttctggt gttgccgtcc ggggtgaagg gggaaaacaa ctggacggtg gagaagaaga
     1561 acgcagtaag actggtcagg gagtaagtga gtatgacaga gctggaaatg cacttactga
     1621 acgccttaga gcaattgcag caggactata cgcagcggct gaaagactgg gagagcgcct
     1681 tcacggattt gcagcatatg ttcgagctga cacgacagga gaacgggcaa ctgagggaac
     1741 agtgcgacga cttgagtcag caggtgcaga gattagccgg gcaggtggac cacttgagtc
     1801 ggttatttat cacgaacagc agataaggga tgaacaggaa aggcaggcac gcctggaacg
     1861 ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa gcacgggtat ggcgtggccc
     1921 gtcaataggg atgtgacggt gagttgttcc gggaggttgc cagatggtac cggaacgcgt
     1981 taaaaacgca tccgggcgcg attctggcgt gtcgtttttg ttgctgtgtt ttgcctcttt
     2041 gccagatcgc gctcattcct cagcctcatc ccgcgatgtc tgcgtatgtc atcaactgaa
     2101 aagatggaaa aaaaacagtg tcatgaggtt tacgcaggca aaactagcgt tattcacatg
     2161 gctgaatagc tatgcatacg gttaaaattt atcaggtgcg atcgcggcag cttttcgggt
     2221 ggtttgttgc catttttacc tgtcagcagc cgtgatcgcg ctgaacgcat ttcagcggtg
     2281 cgtacaatta agggattatg gtaaatccct tactgtctgc cctcgtatcc atcgagataa
     2341 accgcagaaa tggtgtctgc cattagtttc atttttttcg gcgaaggcat atcgctgttt
     2401 gtgtattttc tgccagcaaa ggctgttgtg ctccggagtg agtgaaaaaa tttttctgag
     2461 tggaattctt ttacacgttg ctgtgccgtt tctgctgata ctccggctgc tttgtctttc
     2521 atccccatat cccggaactg ctgaaagaac gggagcatca tacgtatggt gtctgctttc
     2581 tgggtgtaga agttaggatc aaatccatca tctgaagcat aaacataatg ccgggtatgt
     2641 cgttccaggc tggtctttgg tgtgcatcct gtaagagcta ttgttgctgt tatcagaggt
     2701 atcagaagca gcctggcagc ggcagatctc ataaacatct ccttgcgttt ttaatcttga
     2761 tactcaggga gttatatctc cgaagagtat ctcttatcat acgtcggttt actcccaatg
     2821 tgatttgatg gtcaatgagt caggaataat cccgtgaaaa aatgtgtgcc agtcctgctt
     2881 tttagtgttc tttctgcttc ggttgccagt catgcggaca gcaatgtttt tcaacaaaag
     2941 gccgagaaga caaaaaacgg cattgttgcg gatgttaatg ccagacagaa aatgttcagt
     3001 aacagactga agatacaact tcagatcgat gagttgagag cagagctggc tggcaccaga
     3061 gatgcccaaa aaagagagga tttgcgtaaa aaactggagt ctctggagcg gcaaaagaaa
     3121 tccttacgct gatgggggcg tattttcccc ggtttcttcc ataacctttt taccgttcag
     3181 atatttacgg gcgaggcgaa tgcgtcttgc ctggtatacc gccgagtaag cctgggtggg
     3241 attcacacca ttactgattt cactgaccac atgacgctga tcagccagtg tcagcgtgta
     3301 aacctgtttc tggatgaggt cacgggcttt gtcatgctca tatccggcag caacaagggt
     3361 gtcgagaagt cgtttgtctg ttttgttcag tttatggcgg ggtgatttgc tcacatcgtt
     3421 ctccgttttt tcatgatcca acagttttat atctcatcct gatttgctcc ggtgttctgt
     3481 ttcgacgttc tcaatgcctg cctccccata gtgtgatcct tattgcaaaa atgaaaatcc
     3541 atgctcttga catggacaat gctgagtagt aggttttact gtatataaaa ccagtggtta
     3601 tatatacagt atattgtttt taatttattg ttttaattat caaagaggaa tttatggcta
     3661 aagaactaag tggatatgga ccaactgctg gtgagtctat ggggggaaca ggggcgaatc
     3721 tgaatcagca aggtggaaat aataatagta atagtggggt tcactggggt ggtggctctg
     3781 ggcatggtaa taatggtggt cagggaaatt caaattcatc tggttctaca tcaactgtga
     3841 tgaaaaccgg tgagtcatat ctgactccgt ggggtgatgt tgtcattaat aatgatggcc
     3901 ttccggtgat gaatggtatt gttatgacag aagagaattc aactctggtt gataatccat
     3961 ttggaggggt ctccagagtt cttaattctt taattagtga tatgccatca ctttttgcag
     4021 aaagctcagg aaataacaac aataacactg cttcagttaa tactgcacca acgaatgctc
     4081 aggtcagtga tatggataag agttcgaagg tggtgggtaa tgtaattaat gaaaaacaaa
     4141 agcagaagaa taaaattgct acacagatta gtgaaaaaca gaagaaaatc gaggagatga
     4201 aaaaagtatt caagcatcat tcttatcatg gtatcactga tcttgagagg gatgttgatg
     4261 agttgcaaaa aaaatcaaat cagcttgatg cagatattag caaacttaat tcttataaaa
     4321 atacattgca aagtaaaatt ggtgatgtga ataaacagaa ggaagctgaa gaaaaggctc
     4381 gtgaaaatgc agaggtcgct gaacatgaaa ctcttaatga agagaaacag gcggtggcgg
     4441 aagcagagaa gcgtttagct gaggcgaaag ctgaactggc gaaggctgaa agtgatgtac
     4501 agagtaagca agctactgtt tccagagttg caggagagct tgaaaacgct cagaagagtg
     4561 ttgacgtgaa ggtcaccgga tttccgggat ggcgtgacgt tcagaaaaaa ctacagcgtc
     4621 agcttgaagc taagcaggct gagtactccg cagttgaaaa tgaacttaag aatgctgtta
     4681 gtttcaggga tggtaaggcc gctgaggtta aagaggcaga gcagaaatta aaagaagctc
     4741 aggatgctct tgaaaagagc cagataaaag atgctgttga tactatggtt ggattttatc
     4801 aatatataac cgaacaatat ggagaaaaat acgcaaaaat tgctcaggat ttagcggaaa
     4861 agtctaaagg aaagaaaatt caaggtgttg atgaagctct ggcagcattt gaaaagtata
     4921 agaatgtact ggataagaaa ttcagtaagg ttgatcggga tgctattttt aatgcgttag
     4981 agtctgttaa ttatgatgag ttatctaaga atttaacgaa gatatcaaag tcgcttaaaa
     5041 tcaccagtcg agtatctttt ctatatgatg ttggttctga ttttaaaaat gcaatagaaa
     5101 ctggtaactg gcgacctttg tttgtcacgc tggagaaaag tgcggtcgat gtaggtgtgg
     5161 caaaaattgt ggcattaatg tttagtttta ttgttggtgt gcctcttggt ttctggggga
     5221 ttgcaattgt cacaggtatt gtttcttctt acatagggga tgatgagttg agcaaactta
     5281 atgagttgtt agggatttaa tttctcttta gagtgtaata tatgaaactt ggcaatccta
     5341 ctgggattgc cattaataaa taaaacaatg cagataactt gcataagtac cagggttctt
     5401 tttttaactt tgtattttct ttgatatatc tagatatggt taatttagag tatggatata
     5461 gcaatacatg tattgatgat ataaatataa ataacaaagg agtgttatca ttgtcgttaa
     5521 atattaaaat aagaatccat ggtataagtg attcaggtaa attatgtagg tagtatttta
     5581 aatgcataag tatttttact tttgttggaa agaaatatga aaataataat ttttctaatt
     5641 acaatattat tcttgtcagg atgtcaagca aactatattc gtgatgtaca aggaggaacc
     5701 gttgcaccat cttcgtcttc tgaactgacg gggatcgcgg ttcagtagaa aagatcaaag
     5761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cctgtaaacg aaaaaaccgc
     5821 ctggggaggc ggtttagtcg aaggttaagt cagttgggga ctgcttaacc gggtaactgg
     5881 ctttagtgga gcacagatac caaatgctgt ccttccagtg tagccgctat aaggcttcaa
     5941 cttcaagagt tctgcgtatc taaatccact atccagttac caatggctgc tgccagtggc
     6001 gttaagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6061 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6121 ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccaaagg gagaaaggcg
     6181 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gccaccaggg
     6241 ggaaacgcct ggtatcttta agtcctgtcg ggtttcgcca cctctgactt gagcgtcggt
     6301 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagctgc gtggcctttt
     6361 ccgggttctt gttttttgct cacatgtttc ttttggtgtt atcccctgat tctgtggata
     6421 accgcattac cgtctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgga
     6481 gcgagtcagt gagcgaggaa gcggaaaagc gcctggacga gcattttctc cttacgcatc
     6541 tgtgcggcat ttcacacccg gcatggtgca cttttcatac aatcctcatt gatgccaaat
     6601 gattaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     6661 cccgctaaaa cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgctcacag
     6721 gccagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catccccgaa
     6781 gcgtgcgagg cagctgcggt aaagctcgtc agcgtggtcg tgaagcgctt cgcagatgtc
     6841 agcctggtcg tcggcgtcca gttcattcag tttttccagc agggtcagcg tctggcttct
     6901 gatgaagcgg gccatgttaa gggcggtttt ttcctgttta gtcacagttc tgtttctcca
     6961 tgtgtgggga gtctgtcgga ggtttttaat gataccggtg aaacgcgaaa ggatgctcac
     7021 gatacgggtc acggatgagg aacatgcgcg gctgctggca cgttgtgagg gaaaacaact
     7081 tgcgtcatgg atgcgtaagg tctgcctggg ggcaccgcca tcgaaaacgt caggtcttcc
     7141 gacactggca ccgccgctgt tgcgccagtt cgcttcggtc ggcaataacc tgaatcagat
     7201 agcccggaag ataaacagcg gacagtggtc ggggcatgac cgggttcatg tggtggctgc
     7261 actgatggcc atcggacgtg aactgagtga gctgagggat gaggtccgta aacagggaga
     7321 acgggatgat agttaagttt catgccaggg gaaaaggcgg tggcagtggt ccggttgatt
     7381 acctgctggg ccgggagcgt aaccgggaag gggcgagagt tctgcggggt gctcccgaag
     7441 aggtgcggga actcatcgat gccacgccgt ttgcgaaaaa atacacctcg ggggttctgt
     7501 cttttgcaga gcagaccctg ccaccgggag agcgtgaaag ggtgatggag agctttgagc
     7561 gggtactgat gcccggtctt gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg
     7621 acaaggggcg tcttgagctg aattttgtca tcccgaatat ggagctggcg agcggaaaac
     7681 ggctccagcc ctattacgac cgagcagacc gtccgcgaat caatgcctgg cagacactgg
     7741 tgaatcacca ttacgggctg cacgacccga acgcaccgga gaacaggcgg acacttgtca
     7801 ccccgaataa tctgccgaaa gcgaaacagg aggccgcaga agcgattacg aggggactgg
     7861 aggctcttta ccatgccgga gcgctaaaaa cgcgtcagga tgtcactgag gcgcttacag
     7921 cggcaggttt tgaggttgtg aggacaacca ggagcagtat cagtatcgcc gacccggagg
     7981 gtgggcgtaa tctcaggctg aaaggggcgc tgtatgagca atcttttaca aatggggaca
     8041 gacttcgaga agaaactgaa agagcgtgcc gtctctacag agaacatgct gaacagcgag
     8101 ttcaggaagc tcgaagcgtc tgtagacagg gaactgaact taaacgggca gaaaatcaga
     8161 gacgctatca gcgcacacac agcagcggtg aaagaacaac tggagacgct gaacagtgcc
     8221 gtgaacgcgc agttcagcgc cacagagaac gaactggccc ggcggcagga agagctgctc
     8281 tggaagctgg tcaaagggcg gatactgtac ccgtcgctga cggcgctttg cgtcacgggc
     8341 gggatttttc tggcgtcatg ggggctgatt cagtggcagg aaagccggat tgcggcgaac
     8401 atcctggcta tcagggacca ggaagagacg ctggcgaaac tcccgggaaa aaacatgggg
     8461 tgtgacgtat cacgagggca ggaacgggaa atttctggtg ttgccgtccg ggggtgaagg
     8521 gggaaaacaa ctggacggtg gagaagaaga acgcagtaag actggtcagg gagtaagtga
     8581 gtatgacaga gctggaaatg cacttactga acgccttaga gcaattgcag caggactata
     8641 cgcagcggct gaaagactgg gagagcgcct tcacggattt gcagcatatg ttcgagctga
     8701 cacgacagga gaacgggcaa ctgagggaac agtgcgacga cttgagtcag caggtgcaga
     8761 gattagccgg gcaggtggac cacttgagtc ggttatttat cacgaacagc agataaggga
     8821 tgaacaggaa agggcagacg cctggaacgg gagcgtgcgc ggtcctggca tgaagagcag
     8881 gaacgggaag cacgggtatg gcgtggcccg tcaacagggg atgtgacggt gagttgttcc
     8941 gggaggttgc cagatggtac cggaacgcgt taaaaacgca tccgggcgcg attctggcgt
     9001 gtcgtttttg ttgctgtgtt ttgcctcttt gccagatcgc gctcattcct cagcctcatc
     9061 ccgcgatgtc tgcgtatgtc atcaactgaa aagatggaaa aaaacagtgt catgaggttt
     9121 acgcaggcaa aactagcgtt attcacatgg ctgaatagct atgcatacgg ttaaaattta
     9181 tcaggtgcga tcgcggcggc agctttcggg tggttttgtt gccattttaa ccctgtagcg
     9241 gccatgatcg tgtcacaggt attgtttctt cttatgatag ggggtgatga gttgagcaaa
     9301 cttaatgagt tgttagggat ttaatttctc tttggagtgt aatatatgaa acttggcaat
     9361 cctactggga ttgccattaa taaataaaac aatgcagata acttgcataa gtaccagggt
     9421 tcttttttta actttgtatt ttctttgata tatctagata tggttaattt agagtatgga
     9481 tatagcaata catgtattga tgatataaat ataaataaca aaggagtgtt atcattgtcg
     9541 ttaaatatta aaataagaat ccatggtata agtgattcag gtaaattatg taggtagtat
     9601 tttaaatgca taagtatttt tacttttgtt ggaaagaaat atgaaaataa taatttttct
     9661 aattacaata ttattcttgt caggatgtca agcaaactat attcgtgatg tacaaggagg
     9721 aaccgttgca ccatcttcgt cttctgaact gacggggatc gcggttcagt agaaaagatc
     9781 aaaggatctt cttgagatcc tttattctgc gcgtaatctg ctgcctgtaa acgaaaaaac
     9841 cgcctgggga ggcggtttag tcgaaggtta agtcagttgg ggactgctta accgggtaac
     9901 tggctttagt ggagcacaga taccaaatgc tgtccttcca gtgtagccgc tataaggctt
     9961 caacttcaag agttctgcgt atctaaatcc actatccagt taccaatggc tgctgccagt
    10021 ggcgttaagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10081 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10141 gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccaa agggagaaag
    10201 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagccacca
    10261 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10321 ggtttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc tgcgtggcct
    10381 tttccgggtt cttgtttttt gctcacatgt ttcttttggt gttatcccct gattctgtgg
    10441 ataaccgcat taccgtcttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10501 ggagcgagtc agtgagcgag gaagcggaaa agcgcctgga cgagcatttt ctccttacgc
    10561 atctgtgcgg catttcacac ccggcatggt gcacttttca tacaatcctc attgatgcca
    10621 aatgattaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
    10681 acacccgcta aaacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctca
    10741 caggccagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcccc
    10801 gaagcgtgcg aggcagctgc ggtaaagctc gtcagcgtgg tcgtgaagcg cttcgcagat
    10861 gtcagcctgg tcgtcggcgt ccagttcatt cagtttttcc agcagggtca gcgtctggct
    10921 tctgatgaag cgggccatgt taagggcggt tttttcctgt ttagtcacag ttctgtttct
    10981 ccatgtgtgg ggagtctgtc ggaggttttt aatgataccg gtgaaacgcg aaaggatgct
    11041 cacgatacgg gtcacggatg aggaacatgc gcggctgctg gcacgttgtg agggaaaaca
    11101 acttgcgtca tggatgcgta aggtctgcct gggggcaccg ccatcgaaaa cgtcaggtct
    11161 tccgacactg gcaccgccgc tgttgcgcca gttcgcttcg gtcggcaata acctgaatca
    11221 gatagcccgg aagataaaca gcggacagtg gtcggggcat gaccgggttc atgtggtggc
    11281 tgcactgatg gccatcggac gtgaactgag tgagctgagg gatgaggtcc gtaaacaggg
    11341 agaacgggat gatagttaag tttcatgcca ggggaaaagg cggtggcagt ggtccggttg
    11401 attacctgct gggccgggag cgtaaccggg aaggggcgag agttctgcgg ggtgctcccg
    11461 aagaggtgcg ggaactcatc gatgccacgc cgtttgcgaa aaaatacacc tcgggggttc
    11521 tgtcttttgc agagcagacc ctgccaccgg gagagcgtga aagggtgatg gagagctttg
    11581 agcgggtact gatgcccggt cttgaaaaaa atcagtacag catcctgtgg gtggagcacc
    11641 aggacaaggg gcgtcttgag ctgaattttg tcatcccgaa tatggagctg gcgagcggaa
    11701 aacggctcca gccctattac gaccgagcag accgtccgcg aatcaatgcc tggcagacac
    11761 tggtgaatca ccattacggg ctgcacgacc cgaacgcacc ggagaacagg cggacacttg
    11821 tcaccccgaa taatctgccg aaagcgaaac aggaggccgc agaagcgatt acgaggggac
    11881 tggaggctct ttaccatgcc ggagcgctaa aaacgcgtca ggatgtcact gaggcgctta
    11941 cagcggcagg ttttgaggtt gtgaggacaa ccaggagcag tatcagtatc gccgacccgg
    12001 agggtgggcg taatctcagg ctgaaagggg cgctgtatga gcaatctttt acaaatgggg
    12061 acagacttcg agaagaaact gaaagagcgt gccgtctcta cagagaacat gctgaacagc
    12121 gagttcagga agctcgaagc gtctgtagac agggaactga acttaaacgg gcagaaaatc
    12181 agagacgcta tcagcgcaca cacagcagcg gtgaaagaac aactggagac gctgaacagt
    12241 gccgtgaacg cgcagttcag cgccacagag aacgaactgg cccggcggca ggaagagctg
    12301 ctctggaagc tggtcaaagg gcggatactg tacccgtcgc tgacggcgct ttgcgtcacg
    12361 ggcgggattt ttctggcgtc atgggggctg attcagtggc aggaaagccg gattgcggcg
    12421 aacatcctgg ctatcaggga ccaggaagag acgctggcga aactccggga aaaaacatgg
    12481 ggtgtgacgt atcacgaggg caggaacggg aaatttctgg tgttgccgtc cggggtgaag
    12541 ggggaaaaca actggacggt ggagaagaag aacgcagtaa gactggtcag ggagtaagtg
    12601 agtatgacag agctggaaat gcacttactg aacgccttag agcaattgca gcaggactat
    12661 acgcagcggc tgaaagactg ggagagcgcc ttcacggatt tgcagcatat gttcgagctg
    12721 acacgacagg agaacgggca actgagggaa cagtgcgacg acttgagtca gcaggtgcag
    12781 agattagccg ggcaggtgga ccacttgagt cggttattta tcacgaacag cagataaggg
    12841 atgaacagga aaggcaggca cgcctggaac gggagcgtgc gcggtcctgg catgaagagc
    12901 aggaacggga agcacgggta tggcgtggcc cgtcaatagg gatgtgacgg tgagttgttc
    12961 cgggaggttg ccagatggta ccggaacgcg ttaaaaacgc atccgggcgc gattctggcg
    13021 tgtcgttttt gttgctgtgt tttgcctctt tgccagatcg cgctcattcc tcagcctcat
    13081 cccgcgatgt ctgcgtatgt catcaactga aaagatggaa aaaaaacagt gtcatgaggt
    13141 ttacgcaggc aaaactagcg ttattcacat ggctgaatag ctatgcatac ggttaaaatt
    13201 tatcaggtgc gatcgcggca gcttttcggg tggtttgttg ccatttttac ctgtcagcag
    13261 ccgtgatcgc gctgaacgca tttcagcggt gcgtacaatt aagggattat ggtaaatccc
    13321 ttactgtctg ccctcgtatc catcgagata aaccgcagaa atggtgtctg ccattagttt
    13381 catttttttc ggcgaaggca tatcgctgtt tgtgtatttt ctgccagcaa aggctgttgt
    13441 gctccggagt gagtgaaaaa atttttctga gtggaattct tttacacgtt gctgtgccgt
    13501 ttctgctgat actccggctg ctttgtcttt catccccata tcccggaact gctgaaagaa
    13561 cgggagcatc atacgtatgg tgtctgcttt ctgggtgtag aagttaggat caaatccatc
    13621 atctgaagca taaacataat gccgggtatg tcgttccagg ctggtctttg gtgtgcatcc
    13681 tgtaagagct attgttgctg ttatcagagg tatcagaagc agcctggcag cggcagatct
    13741 cataaacatc tccttgcgtt tttaatcttg atactcaggg agttatatct ccgaagagta
    13801 tctcttatca tacgtcggtt tactcccaat gtgatttgat ggtcaatgag tcaggaataa
    13861 tcccgtgaaa aaatgtgtgc cagtcctgct ttttagtgtt ctttctgctt cggttgccag
    13921 tcatgcggac agcaatgttt ttcaacaaaa ggccgagaag acaaaaaacg gcattgttgc
    13981 ggatgttaat gccagacaga aaatgttcag taacagactg aagatacaac ttcagatcga
    14041 tgagttgaga gcagagctgg ctggcaccag agatgcccaa aaagagagga tttgcgtaaa
    14101 aaactggagt ctctggagcg gcaaaagaaa tccttacgct gatgggggcg tattttcccc
    14161 ggtttcttcc ataacctttt taccgttcag atatttacgg gcgaggcgaa tgcgtcttgc
    14221 ctggtatacc gccgagtaag cctgggtggg attcacacca ttactgattt cactgaccac
    14281 atgacgctga tcagccagtg tcagcgtgta aacctgtttc tggatgaggt cacgggcttt
    14341 gtcatgctca tatccggcag caacaagggt gtcgagaagt cgtttgtctg ttttgttcag
    14401 tttatggcgg ggtgatttgc tcacatcgtt ctccgttttt tcatgatcca acagttttat
    14461 atctcatcct gatttgctcc ggtgttctgt ttcgacgttc tcaatgcctg cctccccata
    14521 gtgtgatcct tattgcaaaa atgaaaatcc atgctcttga catggacaat gctgagtagt
    14581 aggttttact gtatataaaa ccagtggtta tatatacagt atattgtttt taatttattg
    14641 ttttaattat caaagaggaa tttatggcta aagaactaag tggatatgga ccaactgctg
    14701 gtgagtctat ggggggaaca ggggcgaatc tgaatcagca aggtggaaat aataatagta
    14761 atagtggggt tcactggggt ggtggctctg ggcatggtaa taatggtggt cagggaaatt
    14821 caaattcatc tggttctaca tcaactgtga tgaaaaccgg tgagtcatat ctgactccgt
    14881 ggggtgatgt tgtcattaat aatgatggcc ttccggtgat gaatggtatt gttatgacag
    14941 aagagaattc aactctggtt gataatccat ttggaggggt ctccagagtt cttaattctt
    15001 taattagtga tatgccatca ctttttgcag aaagctcagg aaataacaac aataacactg
    15061 cttcagttaa tactgcacca acgaatgctc aggtcagtga tatggataag agttcgaagg
    15121 tggtgggtaa tgtaattaat gaaaaacaaa agcagaagaa taaaattgct acacagatta
    15181 gtgaaaaaca gaagaaaatc gaggagatga aaaaagtatt caagcatcat tcttatcatg
    15241 gtatcactga tcttgagagg gatgttgatg agttgcaaaa aaaatcaaat cagcttgatg
    15301 cagatattag caaacttaat tcttataaaa atacattgca aagtaaaatt ggtgatgtga
    15361 ataaacagaa ggaagctgaa gaaaaggctc gtgaaaatgc agaggtcgct gaacatgaaa
    15421 ctcttaatga agagaaacag gcggtggcgg aagcagagaa gcgtttagct gaggcgaaag
    15481 ctgaactggc gaaggctgaa agtgatgtac agagtaagca agctactgtt tccagagttg
    15541 caggagagct tgaaaacgct cagaagagtg ttgacgtgaa ggtcaccgga tttccgggat
    15601 ggcgtgacgt tcagaaaaaa ctacagcgtc agcttgaagc taagcaggct gagtactccg
    15661 cagttgaaaa tgaacttaag aatgctgtta gtttcaggga tggtaaggcc gctgaggtta
    15721 aagaggcaga gcagaaatta aaagaagctc aggatgctct tgaaaagagc cagataaaag
    15781 atgctgttga tactatggtt ggattttatc aatatataac cgaacaatat ggagaaaaat
    15841 acgcaaaaat tgctcaggat ttagcggaaa agtctaaagg aaagaaaatt caaggtgttg
    15901 atgaagctct ggcagcattt gaaaagtata agaatgtact ggataagaaa ttcagtaagg
    15961 ttgatcggga tgctattttt aatgcgttag agtctgttaa ttatgatgag ttatctaaga
    16021 atttaacgaa gatatcaaag tcgcttaaaa tcaccagtcg agtatctttt ctatatgatg
    16081 ttggttctga ttttaaaaat gcaatagaaa ctggtaactg gcgacctttg tttgtcacgc
    16141 tggagaaaag tgcggtcgat gtaggtgtgg caaaaattgt ggcattaatg tttagtttta
    16201 ttgttggtgt gcctcttggt ttctggggga ttgcaattgt cacaggtatt gtttcttctt
    16261 acatagggga tgatgagttg agcaaactta atgagttgtt agggatttaa tttctcttta
    16321 gagtgtaata tatgaaactt ggcaatccta ctgggattgc cattaataaa taaaacaatg
    16381 cagataactt gcataagtac cagggttctt tttttaactt tgtattttct ttgatatatc
    16441 tagatatggt taatttagag tatggatata gcaatacatg tattgatgat ataaatataa
    16501 ataacaaagg agtgttatca ttgtcgttaa atattaaaat aagaatccat ggtataagtg
    16561 attcaggtaa attatgtagg tagtatttta aatgcataag tatttttact tttgttggaa
    16621 agaaatatga aaataataat ttttctaatt acaatattat tcttgtcagg atgtcaagca
    16681 aactatattc gtgatgtaca aggaggaacc gttgcaccat cttcgtcttc tgaactgacg
    16741 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt ttctgcgcgt
    16801 aatctgctgc ctgtaaacga aaaaaccgcc tggggaggcg gtttagtcga aggttaagtc
    16861 agttggggac tgcttaaccg ggtaactggc tttagtggag cacagatacc aaatgctgtc
    16921 cttccagtgt agccgctata aggcttcaac ttcaagagtt ctgcgtatct aaatccacta
    16981 tccagttacc aatggctgct gccagtggcg ttaagtcgtg tcttaccggg ttggactcaa
    17041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    17101 ccagcttgga gcgaacgacc tacaccgaac tgagatacca acagcgtgag ctatgagaaa
    17161 gcgccacgct tcccaaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    17221 caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg
    17281 gtttcgccac ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct
    17341 atggaaaaac gccagctgcg tggccttttc cgggttcttg ttttttgctc acatgtttct
    17401 tttggtgtta tcccctgatt ctgtggataa ccgcattacc gtctttgagt gagctgatac
    17461 cgctcgccgc agccgaacga ccgagcggag cgagtcagtg agcgaggaag cggaaaagcg
    17521 cctggacgag cattttctcc ttacgcatct gtgcggcatt tcacacccgg catggtgcac
    17581 ttttcataca atcctcattg atgccaaatg attaagccag tatacactcc gctatcgcta
    17641 cgtgactggg tcatggctgc gccccgacac ccgctaaaac ccgctgacgc gccctgacgg
    17701 gcttgtctgc tcccggcatc cgctcacagg ccagctgtga ccgtctccgg gagctgcatg
    17761 tgtcagaggt tttcaccgtc atccccgaag cgtgcgaggc agctgcggta aagctcgtca
    17821 gcgtggtcgt gaagcgcttc gcagatgtca gcctggtcgt cggcgtccag ttcattcagt
    17881 ttttccagca gggtcagcgt ctggcttctg atgaagcggg ccatgttaag ggcggttttt
    17941 tcctgtttag tcacagttct gtttctccat gtgtggggag tctgtcggag gttttta
//
