LOCUS       NZ_CP082061             9726 bp    DNA     linear   CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS20675 plasmid pMS20675E.
ACCESSION   NZ_CP082061
VERSION     NZ_CP082061.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934300
            Assembly: GCF_030515235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:32:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,387
            CDSs (total)                      :: 5,264
            Genes (coding)                    :: 5,061
            CDSs (with protein)               :: 5,061
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 65 of 203
            Pseudo Genes (incomplete)         :: 147 of 203
            Pseudo Genes (internal stop)      :: 35 of 203
            Pseudo Genes (multiple problems)  :: 43 of 203
            Pseudo Genes (short protein)      :: 4 of 203
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082061.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS20675"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS20675E"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Adam Irwin"
                     /genotype="ST69"
     gene            <1..915
                     /locus_tag="K6138_RS26890"
                     /old_locus_tag="K6138_26690"
     CDS             <1..915
                     /locus_tag="K6138_RS26890"
                     /old_locus_tag="K6138_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1119..1517)
                     /locus_tag="K6138_RS26895"
                     /old_locus_tag="K6138_26695"
     CDS             complement(1119..1517)
                     /locus_tag="K6138_RS26895"
                     /old_locus_tag="K6138_26695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1694..2620
                     /locus_tag="K6138_RS26900"
                     /old_locus_tag="K6138_26700"
     CDS             1694..2620
                     /locus_tag="K6138_RS26900"
                     /old_locus_tag="K6138_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2623..3156
                     /locus_tag="K6138_RS26905"
                     /old_locus_tag="K6138_26705"
     CDS             2623..3156
                     /locus_tag="K6138_RS26905"
                     /old_locus_tag="K6138_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3222..3431)
                     /locus_tag="K6138_RS26910"
                     /old_locus_tag="K6138_26710"
     CDS             complement(3222..3431)
                     /locus_tag="K6138_RS26910"
                     /old_locus_tag="K6138_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            4509..5474
                     /locus_tag="K6138_RS26915"
                     /old_locus_tag="K6138_26715"
     CDS             4509..5474
                     /locus_tag="K6138_RS26915"
                     /old_locus_tag="K6138_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_302354366.1"
                     /translation="MVVRTQPTTCNCCFSWFFYAALQYFDENLPHRPYHTDDLAFGLRI
                     SGKGRALLARYIQQNQPHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLL
                     YALNIPPGTPPGFLVWGRKNAAGVERSPCEKLCADVNYSGLICKNPFHLEWLVMEWREE
                     AYTLDELADYLDLSASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAV
                     IQRVEMYNASLPVPLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRK
                     GGSKSKRSTVATSARTLKPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(5678..6076)
                     /locus_tag="K6138_RS26920"
                     /old_locus_tag="K6138_26720"
     CDS             complement(5678..6076)
                     /locus_tag="K6138_RS26920"
                     /old_locus_tag="K6138_26720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            6253..7179
                     /locus_tag="K6138_RS26925"
                     /old_locus_tag="K6138_26725"
     CDS             6253..7179
                     /locus_tag="K6138_RS26925"
                     /old_locus_tag="K6138_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            7182..7715
                     /locus_tag="K6138_RS26930"
                     /old_locus_tag="K6138_26730"
     CDS             7182..7715
                     /locus_tag="K6138_RS26930"
                     /old_locus_tag="K6138_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(7781..7990)
                     /locus_tag="K6138_RS26935"
                     /old_locus_tag="K6138_26735"
     CDS             complement(7781..7990)
                     /locus_tag="K6138_RS26935"
                     /old_locus_tag="K6138_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          2..776
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          4566..5335
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1695..2617
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6254..7176
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgacgaa aatttacccc atcgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacata
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgttaa aaatcctgtg
      241 aacggacacg ctcacctgct ctacgcactc aacatcgccg tgagaaccgc gcctgatgcc
      301 tcggttaagg cgctgaaata tgccgcagcg gttgaacgtt cgctgtgtga gaaactttgt
      361 gcggatgtga actacagcgg cctgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggccagc gttctcacag
      601 tggcttgaag cggtgatcca gcgtgtcgaa atgtacaatg catcgcttcc cgttccgctt
      661 tcactggctg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctgca
      781 cgcggtcgca agggcggttc taagtctaag cgcagcacag tagctacatc tgcacgcacg
      841 ctgaaaccgt gggaggcatt agggatcagt cgcgcctggt attaccaact gaaaaaacga
      901 ggtctcgtag agtagaccaa ataagcctat atcagataac agcgcctttc tggcgtcttt
      961 ttgagcagca ggttttttgc cgtttttccc cgttcagccc tgcaatggcg cggctttccg
     1021 cgtgattgtg gtggtagtgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc
     1081 gaggaagcac cagggaatcg gaacacggat ggaggcactt acgcgtctgg gtttggggat
     1141 gtctccagat cgtacttaag ttccttaagt gcttgtaata tcctgtcttt tttatagcat
     1201 agctcgcctg tttttgtgtc taccagtcct gcttcgatca gtagcccagc cgcgagaaag
     1261 agccggtgat tgcgttcccg tcgttgtttt tttccctcga ttcgtttttg gaccgcctgt
     1321 tttcgttcgc tctcctgtgc ctgtaagtcg tttttttccg ctgttgccag tacgtggaga
     1381 agtttgcgtt cctgttctgt gagtccggct tcgttgtcga tcaggtcaat aatctgtctc
     1441 tgtcgtgcgg tctgttctcc gagcttcctg atgaatccga tccgtttctt cagccattcg
     1501 atgttgctga tgcgcatgtt cgtgatgctc cctggtgttt ttttgcggtg tgttgtggtc
     1561 gtgcagtata tcgcttgcgg tcggcgggtg cagggggtat cttccgtgct aattcgtaaa
     1621 attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg cgctcctgca
     1681 gagggctgaa accgtggcaa cctatcacct cagcgttaaa tttggtggca aggggcaggc
     1741 tgcaaaccac gccgattaca tcgagcgcaa agagaaatac cgcgaccgtc aggatttgga
     1801 atattccgca cacggaaaca tgccggaatg ggccagggat aatccttccc atttctggca
     1861 ggcggctgac cagtttgaac gtgcgaacgg ttcgacctat agggaacttg aaattgcatt
     1921 accccgtgaa ctgacaccgg aacagcgcct tgaactggtg caggattttg tacgtcagga
     1981 agctggcgaa cggcacgcct ggagttttgc cattcacaat ccgaaagcga gcattgacgg
     2041 cggcgaacag cctcatgcgc atatcatgat gagtcagcgt gtgaatgatg gcattgaacg
     2101 tacaccggaa cagtatttcc ggcgctataa cgccaggtat cccgagcggg gaggtgcgaa
     2161 aaaggattcc ggaagcctga ctctcactca gcaaaaagag cagttgcggg aattgcgaaa
     2221 acgctgggag gtgaagcaca acgaacatat gagaaagcat ggctttgaaa ggggcttcat
     2281 tgactgccgg acgctgaaag aacagggcat tgaacgacgt cccgaagtgc atctgggttt
     2341 tgaagctgcc ggacggcttg atgatgccca gcgtcatgac atcatgcaaa aacggtctga
     2401 accggactat tcccgccatc ttgaacaggc gcgaacggtc gggcatgaca tcagggaatg
     2461 gctggacact ccggttaagc aacatgaact ggtcagaagg ctgcaacatg atgttgcgcg
     2521 ggagcatggc tttgacatga gagacagagt gagcagacag aaccgaactg cacctgaacg
     2581 ggttaaatca ccgtcacgtg gattttctat gggattctga acatggccag cacagaagaa
     2641 aaattactca cgctcctgtt atcggtggag catctgcaac ataccgcaat ggagcaacag
     2701 aaagctgttg ctgattctct ggctgtggca agggaaactc ttcgcggata tcgggatatt
     2761 tctgacggtg tggcaaaaca ggtcagggat gaagtcagaa atgaggtgca gaagctggac
     2821 gtggcaggac tgataggtga gcgcatcagc caggattttc agcggctgga agagtcagtc
     2881 gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat tcacagcaat
     2941 atcgtgcagg aatacaacac ccttaaaggg tatagctgga aatggctggg tggggtgtca
     3001 gtggcttttc tggttatgtt ctgtgtgctc ggatggggaa tgaaaacaat actcgacagg
     3061 aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga taaactggtg
     3121 gagaaacctg tcacggggaa gagccggaaa aactgacgcg cttcgcttgt ccaacgcctc
     3181 acgacccgcg aaaggatttc gcgaaccgtt caccgttttt ttcaatatga ttctgtctgc
     3241 tttggtcgat aaagcagacg atagtattcc agccttcgta tatataacgg tacgtgctca
     3301 tcagtaagag ctggcggtat tgtgttatca gggggaagtt ttttcagata ctcacgaaat
     3361 gcctggcttg atgccataaa atctacagta gcatcgataa tatgtggggt gttttcacct
     3421 aattttgcca tgatgataat ccttatatta tcacggtttg tgaatgatag ggtgtcacta
     3481 ttatttatgc gttagtgaac cagaaaaaat cccccgttgg cataggggga tatgtaagga
     3541 gaaataactt cgcaggtgtt cttgtttgta taacgtgaaa agactgcacg caacttaata
     3601 agtctgccgt acttactttc agttttcaac ataaggttta taacaatgta tggaatggaa
     3661 gtgagcttta aggtttcagc tctaaaaaat aaaatattta ttagttggct ggttgttgtt
     3721 cttgttctgt atgtatataa acactatcga catattatga ccgggaacat tccttacccc
     3781 ctctgcgctc gcagaggttt tttttgtcat taattaataa tgaactgaaa tgcagttccg
     3841 atacctccat ttgcacatta tccctgacat atggtaccgg aaccgcatta attttatgtt
     3901 ataagagttt atttttaatg tccagatggt ttaatagtat ttatatgtaa ttagctgtat
     3961 ggatagtact ggttttattg tataacttgt cgtttttttt attgtttttg tgtggtgtgc
     4021 taatctttaa gttataaaaa aaccccttgg aatacagggg caaaagtaag gaaaacaaat
     4081 ggatgctcag tttgtgtgac actttaaact aatggaacta tagagtggag gttgttacag
     4141 tcgcttttat aaaacagcaa ccagtttttt tcaatagtct gatgatggtt gtgacagttt
     4201 tgtgatattg ttcaaaatgt ttcttgttga atgtttgttt tttggtctat tggtgtctgg
     4261 ttttattttt tctgctatta catatcaaga aatgtgattg gtgtagcaag agtggaatgc
     4321 gatgtggata aactaattgg ttgtcgaatc taatttactt agaaaactac acttgttttt
     4381 gtgtatgaat ttagtgaaat tgtctgtcta tgtgttttcg tgcatttata aatccttcgt
     4441 caggatctta aatttagtgt tgaatatgta gggcctgtac ggtaattatt atttagcgta
     4501 gctacttcat cgttgttcga acacaaccaa caacctgtaa ttgttgtttc tcttggttct
     4561 tttacgccgc gcttcaatac ttcgacgaaa atttacccca tcgtccctat cacacggatg
     4621 atctcgcttt tggtcttcgc atctccggca aagggcgtgc gcttcttgcg cggtacatac
     4681 agcagaacca gcctcatgcg cagttctggc tggtttttga cgttgaccgc gagggggctg
     4741 cgattgactg gagcgaccgg aacgctcccg cgccgaacat caccgttaaa aatcctgtga
     4801 acggacacgc tcacctgctc tacgcactca acatcccccc ggggaccccc cccgggttcc
     4861 tggtttgggg gcggaaaaat gccgcggggg ttgaacgttc gccgtgtgag aaactttgtg
     4921 cggatgtgaa ctacagcggc ctgatttgca aaaatccgtt ccacctggaa tggctggtga
     4981 tggagtggcg cgaggaagcc tataccctcg atgaactggc tgattatctc gatttgagcg
     5041 cctcagagcg tcgtagcatc gataaacatt acgggatggg gcgaaactgc cacctgttcg
     5101 aaatgacgcg caaatgggct tacagggcga ttcgtcaggg ctggccagcg ttctcacagt
     5161 ggcttgaagc ggtgatccag cgtgtcgaaa tgtacaatgc atcgcttccc gttccgcttt
     5221 cactggctga atgtcgggct attggcaaga gcattgcgaa atacacgcac aggaacttca
     5281 cgccggaaac tttcgcacag tatgtggctg atacgcacac gccagaaatt caggctgcac
     5341 gcggtcgcaa gggcggttct aagtctaagc gcagcacagt agctacatct gcacgcacgc
     5401 tgaaaccgtg ggaggcatta gggatcagtc gcgcctggta ttaccaactg aaaaaacgag
     5461 gtctcgtaga gtagaccaaa taagcctata tcagataaca gcgcctttct ggcgtctttt
     5521 tgagcagcag gttttttgcc gtttttcccc gttcagccct gcaatggcgc ggctttccgc
     5581 gtgattgtgg tggtagtgct cgccgcagtc tcatgaccga gcgtagcgag cgaatgagcg
     5641 aggaagcacc agggaatcgg aacacggatg gaggcactta cgcgtctggg tttggggatg
     5701 tctccagatc gtacttaagt tccttaagtg cttgtaatat cctgtctttt ttatagcata
     5761 gctcgcctgt ttttgtgtct accagtcctg cttcgatcag tagcccagcc gcgagaaaga
     5821 gccggtgatt gcgttcccgt cgttgttttt ttccctcgat tcgtttttgg accgcctgtt
     5881 ttcgttcgct ctcctgtgcc tgtaagtcgt ttttttccgc tgttgccagt acgtggagaa
     5941 gtttgcgttc ctgttctgtg agtccggctt cgttgtcgat caggtcaata atctgtctct
     6001 gtcgtgcggt ctgttctccg agcttcctga tgaatccgat ccgtttcttc agccattcga
     6061 tgttgctgat gcgcatgttc gtgatgctcc ctggtgtttt tttgcggtgt gttgtggtcg
     6121 tgcagtatat cgcttgcggt cggcgggtgc agggggtatc ttccgtgcta attcgtaaaa
     6181 ttagaaggaa gcgcacttat atgtttctct ccttcgtcga gaaacagtgc gctcctgcag
     6241 agggctgaaa ccgtggcaac ctatcacctc agcgttaaat ttggtggcaa ggggcaggct
     6301 gcaaaccacg ccgattacat cgagcgcaaa gagaaatacc gcgaccgtca ggatttggaa
     6361 tattccgcac acggaaacat gccggaatgg gccagggata atccttccca tttctggcag
     6421 gcggctgacc agtttgaacg tgcgaacggt tcgacctata gggaacttga aattgcatta
     6481 ccccgtgaac tgacaccgga acagcgcctt gaactggtgc aggattttgt acgtcaggaa
     6541 gctggcgaac ggcacgcctg gagttttgcc attcacaatc cgaaagcgag cattgacggc
     6601 ggcgaacagc ctcatgcgca tatcatgatg agtcagcgtg tgaatgatgg cattgaacgt
     6661 acaccggaac agtatttccg gcgctataac gccaggtatc ccgagcgggg aggtgcgaaa
     6721 aaggattccg gaagcctgac tctcactcag caaaaagagc agttgcggga attgcgaaaa
     6781 cgctgggagg tgaagcacaa cgaacatatg agaaagcatg gctttgaaag gggcttcatt
     6841 gactgccgga cgctgaaaga acagggcatt gaacgacgtc ccgaagtgca tctgggtttt
     6901 gaagctgccg gacggcttga tgatgcccag cgtcatgaca tcatgcaaaa acggtctgaa
     6961 ccggactatt cccgccatct tgaacaggcg cgaacggtcg ggcatgacat cagggaatgg
     7021 ctggacactc cggttaagca acatgaactg gtcagaaggc tgcaacatga tgttgcgcgg
     7081 gagcatggct ttgacatgag agacagagtg agcagacaga accgaactgc acctgaacgg
     7141 gttaaatcac cgtcacgtgg attttctatg ggattctgaa catggccagc acagaagaaa
     7201 aattactcac gctcctgtta tcggtggagc atctgcaaca taccgcaatg gagcaacaga
     7261 aagctgttgc tgattctctg gctgtggcaa gggaaactct tcgcggatat cgggatattt
     7321 ctgacggtgt ggcaaaacag gtcagggatg aagtcagaaa tgaggtgcag aagctggacg
     7381 tggcaggact gataggtgag cgcatcagcc aggattttca gcggctggaa gagtcagtcg
     7441 caaacatgaa ggcaaacgcg gatgccctga acagggatat tgcgacaatt cacagcaata
     7501 tcgtgcagga atacaacacc cttaaagggt atagctggaa atggctgggt ggggtgtcag
     7561 tggcttttct ggttatgttc tgtgtgctcg gatggggaat gaaaacaata ctcgacagga
     7621 actacaacgc cacagcggcg gtgtatcaga aagttgtgac actggaagat aaactggtgg
     7681 agaaacctgt cacggggaag agccggaaaa actgacgcgc ttcgcttgtc caacgcctca
     7741 cgacccgcga aaggatttcg cgaaccgttc accgtttttt tcaatatgat tctgtctgct
     7801 ttggtcgata aagcagacga tagtattcca gccttcgtat atataacggt acgtgctcat
     7861 cagtaagagc tggcggtatt gtgttatcag ggggaagttt tttcagatac tcacgaaatg
     7921 cctggcttga tgccataaaa tctacagtag catcgataat atgtggggtg ttttcaccta
     7981 attttgccat gatgataatc cttatattat cacggtttgt gaatgatagg gtgtcactat
     8041 tatttatgcg ttagtgaacc agaaaaaatc ccccgttggc atagggggga tatgtaagga
     8101 gaaataactt cgcaggtgtt cttgtttgta taacgtgaaa agactgcacg caacttaata
     8161 agtctgccgt acttactttc agttttcaac ataaggttta taacaatgta tggaatggaa
     8221 gtgagcttta aggtttcagc tctaaaaaat aaaatattta ttagttggct ggttgttgtt
     8281 cttgttctgt atgtatataa acactatcga catattatga ccgggaacat tccttacccc
     8341 ctctgcgctc gcagaggttt tttttgtcat taattaataa tgaactgaaa tgcagttccg
     8401 atacctccat ttgcacatta tccctgacat atggtaccgg aaccgcatta attttatgtt
     8461 ataagagttt atttttaatg tccagatggt ttaatagtat ttatatgtaa ttagctgtat
     8521 ggatagtact ggttttattg tataacttgt cgtttttttt attgtttttg tgtggtgtgc
     8581 taatctttaa gttataaaaa aaccccttgg aatacagggg caaaagtaag gaaaacaaat
     8641 ggatgctcag tttgtgtgac actttaaact aatggaacta tagagtggag gttgttacag
     8701 tcgcttttat aaaacagcaa ccagtttttt tcaatagtct gatgatggtt gtgacagttt
     8761 tgtgatattg ttcaaaatgt ttcttgttga atgtttgttt tttggtctat tggtgtctgg
     8821 ttttattttt tctgctatta catatcaaga aatgtgattg gtgtagcaag agtggaatgc
     8881 gatgtggata aactaattgg ttgtcgaatc taatttactt agaaaactac acttgttttt
     8941 gtgtatgaat ttagtgaaat tgtctgtcta tgtgttttcg tgcatttata aatccttcgt
     9001 caggatctta aatttagtgt tgaatatgta gggcctgtac ggtaattatt atttagcgta
     9061 gctacttcat cgttgttcga acacaaccaa caacctgtaa ttgttgtttc tcttggttcc
     9121 ccacttttgt ggggtttttt atctcttccg tgcatcgtaa agcgcggact ccttctggaa
     9181 aggttgtttt agggcatcac tgaggcattt tttgcgtttg tgaatattga gcatccgata
     9241 actaaaatat ttcccataga ctctgattct tgccaccgac cccaggtcgg tttttttttg
     9301 tcctggcgag atgtgaagtt tggaaacagg aaaccccgtc agagcgccac gcaccagggg
     9361 agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga tcgcacatcg
     9421 ctttcgtgca tagttatgca acgccctgaa aacgattctg acgcgttttt ctggtttggc
     9481 ctggtgtttt cttgtctttt tgcgtttttt gcgccagaaa gcgactgagg gcgttttaag
     9541 gggggcgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt tcgacaggat
     9601 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatc atgtccagat
     9661 ccggcaagat tcaacatgaa atacagaggg cgtgtaggtc gcataacccg gagagaatcg
     9721 taacac
//
