LOCUS       NZ_CP082055             7430 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS20677 plasmid pMS20677B, complete
            sequence.
ACCESSION   NZ_CP082055
VERSION     NZ_CP082055.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934301
            Assembly: GCF_030515215.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:20:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,962
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,639
            CDSs (with protein)               :: 4,639
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 78 of 205
            Pseudo Genes (incomplete)         :: 134 of 205
            Pseudo Genes (internal stop)      :: 36 of 205
            Pseudo Genes (multiple problems)  :: 40 of 205
            Pseudo Genes (short protein)      :: 4 of 205
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082055.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS20677"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS20677B"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Adam Irwin"
                     /genotype="ST1193"
     gene            join(7410..7430,1..2127)
                     /gene="mobQ"
                     /locus_tag="K6139_RS24785"
                     /old_locus_tag="K6139_24585"
     CDS             join(7410..7430,1..2127)
                     /gene="mobQ"
                     /locus_tag="K6139_RS24785"
                     /old_locus_tag="K6139_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_070749924.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NHLINRLNLEIQISHTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKLEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQYPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2375..3076)
                     /locus_tag="K6139_RS24790"
                     /old_locus_tag="K6139_24590"
     CDS             complement(2375..3076)
                     /locus_tag="K6139_RS24790"
                     /old_locus_tag="K6139_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3606..>3731)
                     /gene="mobC"
                     /locus_tag="K6139_RS24795"
                     /old_locus_tag="K6139_24595"
                     /pseudo=""
     CDS             complement(3606..>3731)
                     /gene="mobC"
                     /locus_tag="K6139_RS24795"
                     /old_locus_tag="K6139_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /name="mobC"
     gene            3756..5495
                     /locus_tag="K6139_RS24800"
                     /old_locus_tag="K6139_24600"
     CDS             3756..5495
                     /locus_tag="K6139_RS24800"
                     /old_locus_tag="K6139_24600"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_302359104.1"
                     /translation="MLTTRAVGPAGFGGKVRDWNDRTHAETWRASWADHANRALANAGY
                     QKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQNHLINRLNLEIQISHTEL
                     RNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSNAAWKLTSNAMSMMADMQA
                     TRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWAAPEYRSEQGKIQSLSQQM
                     EKLRQHYRKLEKQDIPASQTAFEKQWQQWGESGLAALRSQLLKREEELRRKELEDTERS
                     RAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLLLHNRNGEYTLWGNELEKY
                     RTRIFESVDLMRDRSGYICDRSEIGQYPPLQRVYSSATFEQLLTQVCQTWPQHTRNLRQ
                     PKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVMSSVDRETRQLQLLLVMGV
                     DDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYAGYGVAVNHDADSHISPAP
                     ELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEWRREEDARTHATERRHDRG
                     WSQ"
                     /name="MobA/MobL family protein"
     gene            complement(5743..6444)
                     /locus_tag="K6139_RS24805"
                     /old_locus_tag="K6139_24605"
     CDS             complement(5743..6444)
                     /locus_tag="K6139_RS24805"
                     /old_locus_tag="K6139_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(6977..7231)
                     /gene="mobC"
                     /locus_tag="K6139_RS24810"
                     /old_locus_tag="K6139_24610"
     CDS             complement(6977..7231)
                     /gene="mobC"
                     /locus_tag="K6139_RS24810"
                     /old_locus_tag="K6139_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          2..338
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.633"
                     /coverage="94.0"
                     /evalue="3.11e-163"
     Typing          complement(7233..7409)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(2377..3076)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5745..6444)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..2124
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.191"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3739..5492
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.114"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaaatca tctccagaag caacggctac agcgccgttg cttctgctgc ctatcgttct
       61 ggttcactaa tgcttgatga gcgtaccgga ctgactcacg actacacccg caaaagcggt
      121 gtggctgagg ctgtgattct gacccccgcg acagcccctg cctggtgtac gaaccgtgcc
      181 gaactctgga atgccgttga gaaggccgaa cgccggaaga actcacaact tgcgcgggaa
      241 attgaactcg ccatccccca cgaactgccg caggatgccg cacgggaaac cgttctcgcc
      301 tttgtcagag aaaactttgt cagtcagggc atgattgctg atgtggcgtt ccatcacatg
      361 gataaaacaa atccccatgc gcacatcatg ctgaccacga gggctgtcgg gcctgctggg
      421 ttcggcggaa aggtcaggga ctggaacgac cggacacatg ccgagacgtg gcgtgcatca
      481 tgggctgacc atgcaaacag ggcactggcg aacgccggtt atcagaaaga gatagaccat
      541 cgttcatacg agcgacaggg gcttgagaaa acgccgggta ttcacctcgg aaagtctgcc
      601 tgtgcgatgg aaacacgcgg tatcgaaaca gaacgcggcg aacagaatca tctgattaac
      661 aggcttaacc tggaaataca gatttcccac accgaactca ggaacagcgg gctgacagag
      721 cctgcccgcc gtgtggctga ccttctgaat atcgggcttc ctgacaacgc aacttcgtac
      781 acactacgtg acatcatcga agcgttgccg aaggacagta acgcggcatg gaaactcacc
      841 agtaacgcca tgtcgatgat ggcagatatg caggcgacgc gcagacgctg ggaagagctg
      901 aacaccgaac gaaaggaagc gatgagccat gctgccagcc tgaaaaaatc atcgccgttt
      961 tcgtccggtt tttcccgtat tcccctgatg caatgggctg caccggaata ccgtagtgag
     1021 cagggaaaaa tccagtctct gagccagcaa atggaaaaat tgcgacagca ttaccggaag
     1081 ctggagaaac aggacattcc cgcgagtcag acggcatttg aaaaacagtg gcagcagtgg
     1141 ggggaatccg gccttgcagc tctcagatcc cagttgctta aacgtgaaga ggaattgcgc
     1201 cggaaggagc tggaggatac agaacgtagt cgcgcggagc agctcaggaa aaatgacaat
     1261 gccgttatgg attcaggggt gctgagagca gtcgttacgg ggtatggcga ggctcccatg
     1321 cctggtaacg gggaaatgac ctgttacctt ctgcttcaca accggaacgg agaatatacg
     1381 ctctggggga acgagctgga gaaataccgc accaggatct ttgagagtgt tgatttaatg
     1441 cgtgacagga gtggttatat ctgtgaccga agcgaaatcg gtcaatatcc accactgcaa
     1501 cgtgtttatt cctctgcgac ctttgagcaa cttctgacgc aggtttgcca gacatggccg
     1561 caacataccc gcaatctccg ccaaccgaag acatggccgg agtctttctg tcttggagaa
     1621 gaccgccagc cagccatgcc atcactggca gcaaggaagg tggattttac gcagggacgt
     1681 ctgccaccga cgctgatgcc ggtcatgagt tctgtcgacc gggaaacccg ccagcttcag
     1741 cttttgctgg tcatgggcgt ggatgactct ctgggcggtg ttgtccggct taatggtacg
     1801 ctgtatcccg cgtttgccgt tccctcagcc gataacagcc agctggtcat cagtgcactg
     1861 accgataagg ggttacggta tgcagggtat ggtgttgccg taaatcatga tgcagacagc
     1921 cacatatcac ctgcgccgga acttatggag tttcatctga aaacccgtga ggcaccgctt
     1981 tttgctgccg tcaacacacc agaaaaacag ccggatcatc tgttccgctc gctgggtttc
     2041 aatcgtacct gggacgaatg gcggcgcgag gaagatgcac gaacacacgc caccgagagg
     2101 agacatgatc gcggttggtc acagtgaatg tgaacaatat tttgttttgt ttaacatcgt
     2161 gttatgttcg tcgatgttca tcgacaggga tgaatgcaag aagcacactc acataacgca
     2221 agcatagttt tgtttttccg cccccaaaaa ttgggggttc ttttttcgct gcatcagagc
     2281 gtttttgacg caatttttca cttcaatagt accttcgcac tactaacaca aaaaaaacgc
     2341 aacagggagt gttttaggtg ccttaaaatc gattctagat cgtcatcttg atctgtttgt
     2401 cttccttgaa gcgaaaatgc agggctacaa ccgtgcgccc ctttttgatc gtctcaaccg
     2461 ttacaaccag atcgcttttt tcgttcagtt cggcgatggc tggcttgatt atcatcttat
     2521 tcaagtcttt gaattgttga tatttatctt caacggcaag catggttcgg aaatcatcga
     2581 tagctatcgt tctgtcaccg gtgctgcgga actgctggag cagctcgtaa atgcggatgc
     2641 tgtaggtgct ggagaggctg gagacgttct tcacgactac ccgcgtaaac tgccccttga
     2701 gctgtgtcag atacggcatg atggcatcgg agaaggtgat ctctactttc gcctcacctt
     2761 tgaaatactg cgcccggtac tgcacccagc ggaactcttc tcgtttttcc tcgttcttga
     2821 tgattactga cctgtcccag aggcgatcta tcgcttcctt gagcctagct tcagcgtgct
     2881 gccgtcccat gtcaggaaag gcatcatgaa actctgcagc cgtgatggtc atggtttttt
     2941 gttcatccgg cgaaggggaa tcttctccgg atttcaggcg gctgatacag accagcagga
     3001 agcgctgttc ctgcaaagtg agcttgtagc ttgcctcgat aagggcgttt gatttgtaag
     3061 ctatcagatc tgacatttcg cttgctcgtt gctattggtg gcgttttttt actgtacgcc
     3121 acccatcata ttggtggaag ttttttttgc aacccctttt ttctgtggat tacaccccca
     3181 aaagctccac ctatcacccc caaaagctcc acctatcacc cccaaaagct ccacctatca
     3241 cccccaaaag ctccacctat ctcgccagaa cacctttaaa atcatgtggt tacaagacct
     3301 aaaaaggttt taaaaaaaaa gatctaaaaa taagatctta aaaacttaac ggcgagcctg
     3361 tggataactt ggggtgctga aaataaaaat ccaataaaaa caatgtgttg aaaaaaatca
     3421 ggattgaaga ttccgaatac acccctccaa ccctgtggaa atccagtatt catgcgggtt
     3481 cagcgcatat cacctgtcga aaaaccgtgc gtatgtgctc aacatggcga ctgattcact
     3541 tccgcttcct cgcccactcg ctcgctaacg ctcggtcgct gggctgcggc gagcggacga
     3601 tacaatcact gctctggcag cgcctccagc tcgttaatca gcggcagtat tcgcgtcaca
     3661 tcagcctgcc gtgtcacctc tgccttcagg aactggagta accaagctcg caactgcact
     3721 tcctccggct ttaaccaaat ccccatgcgc acatcatgct gaccacgagg gctgtcgggc
     3781 ctgctgggtt cggcggaaag gtcagggact ggaacgaccg gacacatgcc gagacgtggc
     3841 gtgcatcatg ggctgaccat gcaaacaggg cactggcgaa cgccggttat cagaaagaga
     3901 tagaccatcg ttcatacgag cgacaggggc ttgagaaaac gccgggtatt cacctcggaa
     3961 agtctgcctg tgcgatggaa acacgcggta tcgaaacaga acgcggcgaa cagaatcatc
     4021 tgattaacag gcttaacctg gaaatacaga tttcccacac cgaactcagg aacagcgggc
     4081 tgacagagcc tgcccgccgt gtggctgacc ttctgaatat cgggcttcct gacaacgcaa
     4141 cttcgtacac actacgtgac atcatcgaag cgttgccgaa ggacagtaac gcggcatgga
     4201 aactcaccag taacgccatg tcgatgatgg cagatatgca ggcgacgcgc agacgctggg
     4261 aagagctgaa caccgaacga aaggaagcga tgagccatgc tgccagcctg aaaaaatcat
     4321 cgccgttttc gtccggtttt tcccgtattc ccctgatgca atgggctgca ccggaatacc
     4381 gtagtgagca gggaaaaatc cagtctctga gccagcaaat ggaaaaattg cgacagcatt
     4441 accggaagct ggagaaacag gacattcccg cgagtcagac ggcatttgaa aaacagtggc
     4501 agcagtgggg ggaatccggc cttgcagctc tcagatccca gttgcttaaa cgtgaagagg
     4561 aattgcgccg gaaggagctg gaggatacag aacgtagtcg cgcggagcag ctcaggaaaa
     4621 atgacaatgc cgttatggat tcaggggtgc tgagagcagt cgttacgggg tatggcgagg
     4681 ctcccatgcc tggtaacggg gaaatgacct gttaccttct gcttcacaac cggaacggag
     4741 aatatacgct ctgggggaac gagctggaga aataccgcac caggatcttt gagagtgttg
     4801 atttaatgcg tgacaggagt ggttatatct gtgaccgaag cgaaatcggt caatatccac
     4861 cactgcaacg tgtttattcc tctgcgacct ttgagcaact tctgacgcag gtttgccaga
     4921 catggccgca acatacccgc aatctccgcc aaccgaagac atggccggag tctttctgtc
     4981 ttggagaaga ccgccagcca gccatgccat cactggcagc aaggaaggtg gattttacgc
     5041 agggacgtct gccaccgacg ctgatgccgg tcatgagttc tgtcgaccgg gaaacccgcc
     5101 agcttcagct tttgctggtc atgggcgtgg atgactctct gggcggtgtt gtccggctta
     5161 atggtacgct gtatcccgcg tttgccgttc cctcagccga taacagccag ctggtcatca
     5221 gtgcactgac cgataagggg ttacggtatg cagggtatgg tgttgccgta aatcatgatg
     5281 cagacagcca catatcacct gcgccggaac ttatggagtt tcatctgaaa acccgtgagg
     5341 caccgctttt tgctgccgtc aacacaccag aaaaacagcc ggatcatctg ttccgctcgc
     5401 tgggtttcaa tcgtacctgg gacgaatggc ggcgcgagga agatgcacga acacacgcca
     5461 ccgagaggag acatgatcgc ggttggtcac agtgaatgtg aacaatattt tgttttgttt
     5521 aacatcgtgt tatgttcgtc gatgttcatc gacagggatg aatgcaagaa gcacactcac
     5581 ataacgcaag catagttttg tttttccgcc cccaaaaatt gggggttctt ttttcgctgc
     5641 atcagagcgt ttttgacgca atttttcact tcaatagtac cttcgcacta ctaacacaaa
     5701 aaaaacgcaa cagggagtgt tttaggtgcc ttaaaatcga ttctagatcg tcatcttgat
     5761 ctgtttgtct tccttgaagc gaaaatgcag ggctacaacc gtgcgcccct ttttgatcgt
     5821 ctcaaccgtt acaaccagat cgcttttttc gttcagttcg gcgatggctg gcttgattat
     5881 catcttattc aagtctttga attgttgata tttatcttca acggcaagca tggttcggaa
     5941 atcatcgata gctatcgttc tgtcaccggt gctgcggaac tgctggagca gctcgtaaat
     6001 gcggatgctg taggtgctgg agaggctgga gacgttcttc acgactaccc gcgtaaactg
     6061 ccccttgagc tgtgtcagat acggcatgat ggcatcggag aaggtgatct ctactttcgc
     6121 ctcacctttg aaatactgcg cccggtactg cacccagcgg aactcttctc gtttttcctc
     6181 gttcttgatg attactgacc tgtcccagag gcgatctatc gcttccttga gcctagcttc
     6241 agcgtgctgc cgtcccatgt caggaaaggc atcatgaaac tctgcagccg tgatggtcat
     6301 ggttttttgt tcatccggcg aaggggaatc ttctccggat ttcaggcggc tgatacagac
     6361 cagcaggaag cgctgttcct gcaaagtgag cttgtagctt gcctcgataa gggcgtttga
     6421 tttgtaagct atcagatctg acatttcgct tgctcgttgc tattggtggc gtttttttac
     6481 tgtacgccac ccatcatatt ggtggaagtt ttttttttgc aacccctttt ttctgtggat
     6541 tacaccccca aaagctccac ctatcacccc caaaagctcc acctatcacc cccaaaagct
     6601 ccacctatca cccccaaaag ctccacctat ctcgccagaa cacctttaaa atcatgtggt
     6661 tacaagacct aaaaaggttt taaaaaaaaa gatctaaaaa taagatctta aaaacttaac
     6721 ggcgagcctg tggataactt ggggtgctga aaataaaaat ccaataaaaa caatgtgttg
     6781 aaaaaaatca ggattgaaga ttccgaatac accccctcca accctgtgga aatccagtat
     6841 tcatgcgggt tcagcgcata tcacctgtcg aaaaaccgtg cgtatgtgct caacatggcg
     6901 actgattcac ttccgcttcc tcgcccactc gctcgctaac gctcggtcgc tgggctgcgg
     6961 cgagcggacg atacaatcac tgctctggca gcgcctccag ctcgttaatc agcggcagta
     7021 ttcgcgtcac atcagcctgc cgtgtcacct ctgccttcag gaactggagt aaccaagctc
     7081 gcaactgcac ttcctccggc tttctgactt ttgccagcaa tgctgcaccc acaatgacct
     7141 tctgcgctgt gtccaggctc cggctcttcg ccttcaggcg ctgtaatctc gcctcagcat
     7201 cagcaatctt ctgttcgagc gttctgctca tgatgtaact ccatgcgcga tgaaaaatcg
     7261 cattttagcg cgtcactggt agtttaaaaa ctgagctggc ataatgcacg gcaaatcacg
     7321 aagtgcgcac ttatacaatc tccactccgt ttcgattgtg tgcgccctgc ggggctgaaa
     7381 gaaaactgca aaaaggcatt acggcagaaa tggcgattta tcatctcagt
//
