LOCUS       NZ_CP082052             1117 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS21199 plasmid pMS21199E, complete
            sequence.
ACCESSION   NZ_CP082052
VERSION     NZ_CP082052.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934302
            Assembly: GCF_030515195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 37x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:37:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,963
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,632
            CDSs (with protein)               :: 4,632
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 80 of 212
            Pseudo Genes (incomplete)         :: 145 of 212
            Pseudo Genes (internal stop)      :: 34 of 212
            Pseudo Genes (multiple problems)  :: 44 of 212
            Pseudo Genes (short protein)      :: 4 of 212
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082052.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS21199"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS21199E"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Adam Irwin"
                     /genotype="ST11637"
ORIGIN
        1 tataaacact atcgacatat tatgaccggg aacattcctt accccctctg cgctcgcaga
       61 ggtttttttt gtcattaatt aataatgaac tgaaatgcag ttccgatagc gcatttgcac
      121 attatccctg acatatggta ccggaaccgc attaatttta tgttataaaa gtttattttt
      181 aatgtccaga tggtttaata gtatttatat gtaattagct gtatggatag tactggtttt
      241 attgtataac ttgtcgtttt ttttattgtt tttgtgtggt gtgctaatct ttaagttata
      301 aaaaaacccc ttggaataca ggggcaaaag taaggaaaac aaatggatgc tcagtttgtg
      361 tgacacttta aactaatgga actatagagt ggaggttgtt acagtcgctt ttataaaaca
      421 gcaaccagtt tttttcaata gtctgatgat ggttgtgaca gttttgtgat attgttcaaa
      481 atgtttcttg ttgaatttcc ggtctattgg tgtctggttt tattttctgc tattacatat
      541 caagaaatgt gattggtgta gcaaaagtgg aatgcgatgt ggataaagct aattggttgt
      601 cgaatctaat ttacttagaa aactacactt gtttttgtgt atgaatttag tgaatttgtc
      661 tgtctatgtg ttttcgtgca tttataaatc cttcgtcagg atcttaaatt tagtgttgaa
      721 tatgtagggc ctgtacggta tcaattattt agcgtagcta cttcatcgtt gttcgaacgc
      781 aaccaacaac ctgtaattgt tgtttctctt ggttccccac tttgtagggt tttttatctg
      841 cgcctgatgc atcgtaaagc gcggactcct tcagaaaggt tgttttaggg catcactgag
      901 gcattttttg cgtttgtgaa tattgagcat ccgataacta aaatatttcc catagactct
      961 gattcttgcc accgacccag gtcggttttt tgtcctggcg agatgtgaag tttcgaaaca
     1021 ggaaacccgt caggcgccac gcaccaggag aaaacttgtc gttttgagcc gttcggtgtt
     1081 cagaacgcac gaaaccgatc gcacatcgct ttcgtgc
//
