LOCUS       NZ_CP082051             2104 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS21199 plasmid pMS21199D, complete
            sequence.
ACCESSION   NZ_CP082051
VERSION     NZ_CP082051.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934302
            Assembly: GCF_030515195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 37x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:37:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,963
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,632
            CDSs (with protein)               :: 4,632
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 80 of 212
            Pseudo Genes (incomplete)         :: 145 of 212
            Pseudo Genes (internal stop)      :: 34 of 212
            Pseudo Genes (multiple problems)  :: 44 of 212
            Pseudo Genes (short protein)      :: 4 of 212
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS21199"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS21199D"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Adam Irwin"
                     /genotype="ST11637"
     gene            1..966
                     /locus_tag="K6131_RS24810"
                     /old_locus_tag="K6131_24630"
     CDS             1..966
                     /locus_tag="K6131_RS24810"
                     /old_locus_tag="K6131_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            970..1239
                     /locus_tag="K6131_RS24815"
                     /old_locus_tag="K6131_24635"
     CDS             970..1239
                     /locus_tag="K6131_RS24815"
                     /old_locus_tag="K6131_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_302361963.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFVEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          395..626
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 atgtcagaag ataaattcct ttcggactac agcccccgtg atgcagtttg ggatacccag
       61 cgcacgctta ccgattctgt cgggggtatc taccagactg ctgctgaatt cgagcgctat
      121 gcactccgta tggcctcctg tagcggtttg ttacgttttg gttggtctac catcatggaa
      181 accggagaaa cgcgcctacg gcttcgtagt gcgcaatttt gccgtgtccg tcattgccct
      241 gtctgccagt ggagaagaac cctcatgtgg caagcccgtt tttatcaggc tctaccgaaa
      301 atcgttgtgg attacccgtc ttcccgatgg ttgtttctga cgttaactgt caggaactgc
      361 gagataggtg aacttggaac agtccttaca gcaatgaatg cggcgtttaa gcgaatggaa
      421 aagcgaaagg agctatcacc tgttcagggg tggatcaggg ctacggaggt gacgcgaggt
      481 aaggatggca gcgcacatcc gcattttcac tgtctgctga tggtgcaacc ttcttggttt
      541 aaagggaaga actacgttaa gcacgaacgt tgggtagaac tctggcgcga ttgcttgcgg
      601 gtgaactatg agccgaatat cgatattcgg gcagtaaaaa ctaagacagg tgaggttgtg
      661 gccaacgttg ccgagcaact gcaaagcgcg gttgctgaaa cgctgaaata ctccgttaaa
      721 ccggaagata tggcaaacga tcctgagtgg tttcttgagc tgacgcggca gcttcacaag
      781 cgccgtttta tctcgaccgg tggggcgcta aaaaacgtcc tccagttgga tcgagaaacc
      841 aatgaggatc ttgtcattgc cgacgatgta ggggatggca ctgatgacgg gaagcggacg
      901 gcgtttgtct gggattcagg taaacggcgt tacaaacgcg cccctgagaa ggataaatcg
      961 gattaacgta tgaatattaa tattgaatac ctgaatggaa ataagactat tggtttattt
     1021 tttttaagaa gtgaagcggt gattcctgac aggtttaaaa accttatttt gcttattgat
     1081 ggattaagtt ttggcacatt tggttttcat ccgcacgaag gttttgttga ggatgaatta
     1141 attttatata ttcagaaaac aaacgagagg gtaaaaactc tttttgtgaa aatagattta
     1201 aacggtatca agtttggatt tttaagaacg cattcttagt tctggaaaag agccagcggc
     1261 aggctgaggt gataggtacg agattgcatg caatctctag tgctctgtct atcctgcatt
     1321 atcctcagca ttatcctcag cattatcctc agccttgcca actcgacacc aatgcaggat
     1381 agacaatccg atgtcaaatg ttaacactct gcgagtggta cattttcccc ggattatcgt
     1441 cctgagcctg ccgctggctc tctttctacc gcctcgcttt gctcgttgct caacgcctca
     1501 cagacacgga ttaaaatccg catccgttca ccgtttttta aagtccgtta aaagcatgat
     1561 gccatctccg agagttaatc tcgtcaaatg ctaaatcgtg ggggtcccct ttggggttcc
     1621 gatttagtga ttgacgacac caccgattaa aaaacttatg cggggtggat ggtttcacga
     1681 agtgaggcca tccacctgta agacagggtt ttgtttttat tccctgtttt ggtgatcggg
     1741 tgtgtggaaa aggttggggt aagccgttcg ggggtgcttg ttttgggggg ttaaaattgt
     1801 ggttattttt tgcgcaattc tcgcgcgtga tccttgtatt tatacttaag ggataaatgg
     1861 cggatatgaa atagtggttt agcccagtaa tgacgaggct ttgagtgggt tttgacaggt
     1921 caaagaaaat ggagcagaat tgaggcgttt ttaatcggcg ttggggagtg cgtcaacact
     1981 ccccaacatt tcgaatgtgt cacctcagcg gcaaactctg gtgacatgta ctggctcgca
     2041 atgcacaggt acgtgatgaa tataccacat caaatcacag cctgcccaga tcggagcagg
     2101 ctta
//
