LOCUS       NZ_CP082050            13179 bp    DNA     circular CON 23-JUL-2023
DEFINITION  Escherichia coli strain MS21199 plasmid pMS21199C, complete
            sequence.
ACCESSION   NZ_CP082050
VERSION     NZ_CP082050.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20934302
            Assembly: GCF_030515195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Neonatal meningitis Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13179)
  AUTHORS   Nhu,N.T.K., Phan,M.-D., Brauner,A., Westerlund-Wilkstrom,B.,
            Irwin,A.D. and Schembri,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) School of Chemistry and Molecular
            Biosciences, University of Queensland, Building 76 Cooper road,
            Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 37x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/21/2023 17:37:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,963
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,632
            CDSs (with protein)               :: 4,632
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 80 of 212
            Pseudo Genes (incomplete)         :: 145 of 212
            Pseudo Genes (internal stop)      :: 34 of 212
            Pseudo Genes (multiple problems)  :: 44 of 212
            Pseudo Genes (short protein)      :: 4 of 212
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS21199"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMS21199C"
                     /geo_loc_name="Australia"
                     /collection_date="2019"
                     /collected_by="Adam Irwin"
                     /genotype="ST11637"
     gene            1..210
                     /locus_tag="K6131_RS24740"
                     /old_locus_tag="K6131_24560"
     CDS             1..210
                     /locus_tag="K6131_RS24740"
                     /old_locus_tag="K6131_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(276..809)
                     /locus_tag="K6131_RS24745"
                     /old_locus_tag="K6131_24565"
     CDS             complement(276..809)
                     /locus_tag="K6131_RS24745"
                     /old_locus_tag="K6131_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(812..>1144)
                     /locus_tag="K6131_RS24750"
                     /old_locus_tag="K6131_24570"
                     /pseudo=""
     CDS             complement(812..>1144)
                     /locus_tag="K6131_RS24750"
                     /old_locus_tag="K6131_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     gene            complement(<1143..1754)
                     /locus_tag="K6131_RS24755"
                     /old_locus_tag="K6131_24575"
                     /pseudo=""
     CDS             complement(<1143..1754)
                     /locus_tag="K6131_RS24755"
                     /old_locus_tag="K6131_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3492..3702
                     /locus_tag="K6131_RS24760"
                     /old_locus_tag="K6131_24580"
                     /pseudo=""
     CDS             3492..3702
                     /locus_tag="K6131_RS24760"
                     /old_locus_tag="K6131_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3768..4301)
                     /locus_tag="K6131_RS24765"
                     /old_locus_tag="K6131_24585"
     CDS             complement(3768..4301)
                     /locus_tag="K6131_RS24765"
                     /old_locus_tag="K6131_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4304..5230)
                     /locus_tag="K6131_RS24770"
                     /old_locus_tag="K6131_24590"
     CDS             complement(4304..5230)
                     /locus_tag="K6131_RS24770"
                     /old_locus_tag="K6131_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            5407..5805
                     /locus_tag="K6131_RS24775"
                     /old_locus_tag="K6131_24595"
     CDS             5407..5805
                     /locus_tag="K6131_RS24775"
                     /old_locus_tag="K6131_24595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(6009..6353)
                     /locus_tag="K6131_RS24780"
                     /old_locus_tag="K6131_24600"
     CDS             complement(6009..6353)
                     /locus_tag="K6131_RS24780"
                     /old_locus_tag="K6131_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_302361961.1"
                     /translation="MSRSGKIQHEWLEAVIQRVEMYNASLPVPLSLAECRAIGKSIAKY
                     THRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTLKPWEALGISRAWY
                     YQLKKRGLVE"
                     /name="hypothetical protein"
     gene            8014..8223
                     /locus_tag="K6131_RS24785"
                     /old_locus_tag="K6131_24605"
     CDS             8014..8223
                     /locus_tag="K6131_RS24785"
                     /old_locus_tag="K6131_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(8289..8822)
                     /locus_tag="K6131_RS24790"
                     /old_locus_tag="K6131_24610"
     CDS             complement(8289..8822)
                     /locus_tag="K6131_RS24790"
                     /old_locus_tag="K6131_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(8825..9751)
                     /locus_tag="K6131_RS24795"
                     /old_locus_tag="K6131_24615"
     CDS             complement(8825..9751)
                     /locus_tag="K6131_RS24795"
                     /old_locus_tag="K6131_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            9928..10326
                     /locus_tag="K6131_RS24800"
                     /old_locus_tag="K6131_24620"
     CDS             9928..10326
                     /locus_tag="K6131_RS24800"
                     /old_locus_tag="K6131_24620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(10530..11444)
                     /locus_tag="K6131_RS24805"
                     /old_locus_tag="K6131_24625"
     CDS             complement(10530..11444)
                     /locus_tag="K6131_RS24805"
                     /old_locus_tag="K6131_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     Typing          complement(1145..1754)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.218"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(6010..6325)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.93e-165"
     Typing          complement(10670..11444)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(816..1144)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="100.0"
                     /evalue="1e-62"
     Typing          complement(4308..5230)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8829..9751)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaaat taggtgaaaa caccccacat attatcgatg ctactgtaga ttttatggca
       61 tcaagccagg catttcgtga gtatctgaaa aaacttcccc ctgataacac aataccgcca
      121 gctcttactg atgagcacgt accgttatat ataagaaggc tggaatacta tcgtctgctt
      181 tatcgaccaa agcagacaga atcatattga aaaaaacggt gaacggttcg cgaaattctt
      241 tcgcgggtcg tgaggcgttg gacaagcgaa gcgcgtcagt ttttccggct cttccccgtg
      301 acaggtttct ccaccagttt atcttccagt gtcacaactt tctgatacac cgccgctgtg
      361 gcgttgtagt tcctgtcgag tattgttttc attccccatc cgagcacaca gaacataacc
      421 agaaaagcca ctgacacccc acccagccat ttccagctat accctttaag ggtgttgtat
      481 tcctgcacga tattgctgtg aattgtcgca atatccctgt tcagggcatc cgcgtttgcc
      541 ttcatgtttg cgactgactc ttccagccgc tgaaaatcct ggctgatgcg ctcacctatc
      601 agtcctgcca cgtccagctt ctgcacctca tttctgactt catccctgac ctgttttgcc
      661 acaccgtcag aaatatcccg atatccgcga agagtttccc ttgccacagc cagagaatca
      721 gcaacagctt tctgttgctc cattgcggta tgttgcagat gctccaccga taacaggagc
      781 gtaagtaatt tttcttctgt gctggccatg ttcagaatcc catagaaaat ccacgtgacg
      841 gtgatttaac ccgttcaggt gcagttcggt tctgtctgct cactctgtct ctcatgtcaa
      901 agccatgctc ccgcgcaaca tcatgttgca gccttctgac cagttcatgt tgcttaaccg
      961 gagtgtccag ccattccctg atgtcatgcc cgaccgttcg cgcctgttca agatggcggg
     1021 aatagtccgg ttcagaccgt ttttgcatga tgtcatgacg ctgggcatca tcaagccgtc
     1081 cggcagcttc aaaacccaga tgcacttcgg gacgtcgttc aatgccctgt tctttcagcg
     1141 tccgcttcaa gccactgtga gaacgctggc cagccctgac gaatcgccct gtaagcccat
     1201 ttgcgcgtca tttcgaacag gtggcagttt cgccccatcc cgtaatgttt atcgatgcta
     1261 cgacgctctg aggcgctcaa atcgagataa tcagccagtt catcgagggt ataggcttcc
     1321 tcgcgccact ccatcaccag ccattccagg tggaacggat ttttgcaaat caggccgctg
     1381 tagttcacat ccgcacaaag tttctcacac agcgaacgtt caaccgctgc ggcatatttc
     1441 agcgccttaa ccgaggcatc aggcgcggtt ctcacggcga tgttgagtgc gtagagcagg
     1501 tgagcgtgtc cgttcacagg atttttaacg gtgatgttcg gcgcgggagc gttccggtcg
     1561 ctccagtcaa tcgcagcccc ctcgcggtca acgtcaaaaa ccagccagaa ctgcgcatga
     1621 ggctggttct gctgtatgta ccgcgcaaga agcgcacgcc ctttgccgga gatgcgaaga
     1681 ccaaaagcga gatcatccgt gtgataggga cgatggggta aattttcgtc gaagtattga
     1741 agcgcggcgc tcatgtgtta cgattctctc cgggttatgc gacctacacg ccctctgtat
     1801 ttcatgttga atcttgccgg atctggacat gatttcaggg gcgtttgttt ttcagtcgct
     1861 tacactacac ccaacggcaa atcctgtcga accttcccga aaacccgatc catttaccat
     1921 aactcccgtt gtacgccccc cttaaaacgc cctcagtcgc tttctggcgc aaaaaacgca
     1981 aaaagacaag aaaacaccag gccaaaccag aaaaacgcgt cagaatcgtt ttcagggcgt
     2041 tgcataacta tgcacgaaag cgatgtgcga tcggtttcgt gcgttctgaa caccgaacgg
     2101 ctcaaaacga caagttttct cccctggtgc gtggcgctct gacggggttt cctgtttcca
     2161 aacttcacat ctcgccagga caaaaaaaaa ccgacctggg gtcggtggca agaatcagag
     2221 tctatgggaa atattttagt tatcggatgc tcaatattca caaacgcaaa aaatgcctca
     2281 gtgatgccct aaaacaacct ttccagaagg agtccgcgct ttacgatgca cggaagagat
     2341 aaaaaacccc acaaaagtgg ggaaccaaga gaaacaacaa ttacaggttg ttggttgtgt
     2401 tcgaacaacg atgaagtagc tacgctaaat aataattacc gtacaggccc tacatattca
     2461 acactaaatt taagatcctg acgaaggatt tataaatgca cgaaaacaca tagacagaca
     2521 aattcactaa attcatacac aaaaacaagt gtagttttct aagtaaatta gattcgacaa
     2581 ccaattagtt tatccacatc gcattccact cttgctacac caatcacatt tcttgatatg
     2641 taatagcaga aaaaataaaa ccagacacca atagaccaaa aaacaaacat tcaacaagaa
     2701 acattttgaa caatatcaca aaactgtcac aaccatcatc agactattga aaaaaactgg
     2761 ttgctgtttt ataaaagcga ctgtaacaac ctccactcta tagttccatt agtttaaagt
     2821 gtcacacaaa ctgagcatcc atttgttttc cttacttttg cccctgtatt ccaaggggtt
     2881 tttttataac ttaaagatta gcacaccaca caaaaacaat aaaaaaaacg acaagttata
     2941 caataaaacc agtactatcc atacagctaa ttacatataa atactattaa accatctgga
     3001 cattaaaaat aaactcttat aacataaaat taatgcggtt ccggtaccat atgtcaggga
     3061 taatgtgcaa atggaggtat cggaactgca tttcagttca ttattaatta atgacaaaaa
     3121 aaacctctgc gagcgcagag ggggtaagga atgttcccgg tcataatatg tcgatagtgt
     3181 ttatatacat acagaacaag aacaacaacc agccaactaa taaatatttt attttttaga
     3241 gctgaaacct taaagctcac ttccattcca tacattgtta taaaccttat gttgaaaact
     3301 gaaagtaagt acggcagact tattaagttg cgtgcagtct tttcacgtta tacaaacaag
     3361 aacacctgcg aagttatttc tccttacata tcccccctat gccaacgggg gatttttttc
     3421 tggttcacta acgcataaat aatagtgaca ccctatcatt cacaaaccgt gataatataa
     3481 ggattatcat catggcaaaa ttaggtgaaa acaccccaca tattatcgat gctactgtag
     3541 attttatggc atcaagccag gcatttcgtg agtatctgaa aaaacttccc ccctgataac
     3601 acaataccgc cagctcttac tgatgagcac gtaccgttat atataagaag gctggaatac
     3661 tatcgtctgc tttatcgacc aaagcagaca gaatcatatt gaaaaaaacg gtgaacggtt
     3721 cgcgaaattc tttcgcgggt cgtgaggcgt tggacaagcg aagcgcgtca gtttttccgg
     3781 ctcttccccg tgacaggttt ctccaccagt ttatcttcca gtgtcacaac tttctgatac
     3841 accgccgctg tggcgttgta gttcctgtcg agtattgttt tcattcccca tccgagcaca
     3901 cagaacataa ccagaaaagc cactgacacc ccacccagcc atttccagct atacccttta
     3961 agggtgttgt attcctgcac gatattgctg tgaattgtcg caatatccct gttcagggca
     4021 tccgcgtttg ccttcatgtt tgcgactgac tcttccagcc gctgaaaatc ctggctgatg
     4081 cgctcaccta tcagtcctgc cacgtccagc ttctgcacct catttctgac ttcatccctg
     4141 acctgttttg ccacaccgtc agaaatatcc cgatatccgc gaagagtttc ccttgccaca
     4201 gccagagaat cagcaacagc tttctgttgc tccattgcgg tatgttgcag atgctccacc
     4261 gataacagga gcgtaagtaa tttttcttct gtgctggcca tgttcagaat cccatagaaa
     4321 atccacgtga cggtgattta acccgttcag gtgcagttcg gttctgtctg ctcactctgt
     4381 ctctcatgtc aaagccatgc tcccgcgcaa catcatgttg cagccttctg accagttcat
     4441 gttgcttaac cggagtgtcc agccattccc tgatgtcatg cccgaccgtt cgcgcctgtt
     4501 caagatggcg ggaatagtcc ggttcagacc gtttttgcat gatgtcatga cgctgggcat
     4561 catcaagccg tccggcagct tcaaaaccca gatgcacttc gggacgtcgt tcaatgccct
     4621 gttctttcag cgtccggcag tcaatgaagc ccctttcaaa gccatgcttt ctcatatgtt
     4681 cgttgtgctt cacctcccag cgttttcgca attcccgcaa ctgctctttt tgctgagtga
     4741 gagtcaggct tccggaatcc tttttcgcac ctccccgctc gggatacctg gcgttatagc
     4801 gccggaaata ctgttccggt gtacgttcaa tgccatcatt cacacgctga ctcatcatga
     4861 tatgcgcatg aggctgttcg ccgccgtcaa tgctcgcttt cggattgtga atggcaaaac
     4921 tccaggcgtg ccgttcgcca gcttcctgac gtacaaaatc ctgcaccagt tcaaggcgct
     4981 gttccggtgt cagttcacgg ggtaatgcaa tttcaagttc cctataggtc gaaccgttcg
     5041 cacgttcaaa ctggtcagcc gcctgccaga aatgggaagg attatccctg gcccattccg
     5101 gcatgtttcc gtgtgcggaa tattccaaat cctgacggtc gcggtatttc tctttgcgct
     5161 cgatgtaatc ggcgtggttt gcagcctgcc ccttgccacc aaatttaacg ctgaggtgat
     5221 aggttgccac ggtttcagcc ctctgcagga gcgcactgtt tctcgacgaa ggagagaaac
     5281 atataagtgc gcttccttct aattttacga attagcacgg aagatacccc ctgcacccgc
     5341 cgaccgcaag cgatatactg cacgaccaca acacaccgca aaaaaacacc agggagcatc
     5401 acgaacatgc gcatcagcaa catcgaatgg ctgaagaaac ggatcggatt catcaggaag
     5461 ctcggagaac agaccgcacg acagagacag attattgacc tgatcgacaa cgaagccgga
     5521 ctcacagaac aggaacgcaa acttctccac gtactggcaa cagcggaaaa aaacgactta
     5581 caggcacagg agagcgaacg aaaacaggcg gtccaaaaac gaatcgaggg aaaaaaacaa
     5641 cgacgggaac gcaatcaccg gctctttctc gcggctgggc tactgatcga agcaggactg
     5701 gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca agcacttaag
     5761 gaacttaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc tccatccgtg
     5821 ttccgattcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat gagactgcgg
     5881 cgagcactac caccacaatc acgcggaaag ccgcgccatt gcagggctga acggggaaaa
     5941 acggcaaaaa acctgctgct caaaaagacg ccagaaaggc gctgttatct gatataggct
     6001 tatttggtct actctacgag acctcgtttt ttcagttggt aataccaggc gcgactgatc
     6061 cctaatgcct cccacggttt cagcgtgcgt gcagatgtag ctactgtgct gcgcttagac
     6121 ttagaaccgc ccttgcgacc gcgtgcagcc tgaatttctg gcgtgtgcgt atcagccaca
     6181 tactgtgcga aagtttccgg cgtgaagttc ctgtgcgtgt atttcgcaat gctcttgcca
     6241 atagcccgac attcagccag tgaaagcgga acgggaagcg atgcattgta catttcgaca
     6301 cgctggatca ccgcttcaag ccactcatgt tgaatcttgc cggatctgga catgatttca
     6361 ggggcgtttg tttttcagtc gcttacacta cacccaacgg caaatcctgt cgaaccttcc
     6421 cgaaaacccg atccatttac cataactccc gttgtacgcc ccccttaaaa cgccctcagt
     6481 cgctttctgg cgcaaaaaac gcaaaaagac aagaaaacac caggccaaac cagaaaaaac
     6541 gcgtcagaat cgttttcagg gcgttgcata actatgcacg aaagcgatgt gcgatcggtt
     6601 tcgtgcgttc tgaacaccga acggctcaaa acgacaagtt ttctcccctg gtgcgtggcg
     6661 ctctgacggg gtttcctgtt tccaaacttc acatctcgcc aggacaaaaa aaaccgacct
     6721 ggggtcggtg gcaagaatca gagtctatgg gaaatatttt agttatcgga tgctcaatat
     6781 tcacaaacgc aaaaaaatgc ctcagtgatg ccctaaaaca acctttccag aagggggcgc
     6841 ccgcttttcc gcgcacagaa aaaaaaaaaa aaccccaaag agggggggca agaaaaacaa
     6901 caattagggg gggttggtgg ggttcgaaca acgatgaagt agctacgcta aataataatt
     6961 accgtacagg ccctacatat tcaacactaa atttaagatc ctgacgaagg atttataaat
     7021 gcacgaaaac acatagacag acaaattcac taaattcata cacaaaaaca agtgtagttt
     7081 tctaagtaaa ttagattcga caaccaatta gtttatccac atcgcattcc actcttgcta
     7141 caccaatcac atttcttgat atgtaatagc agaaaaaata aaaccagaca ccaatagacc
     7201 aaaaaacaaa cattcaacaa gaaacatttt gaacaatatc acaaaactgt cacaaccatc
     7261 atcagactat tgaaaaaaac tggttgctgt tttataaaag cgactgtaac aacctccact
     7321 ctatagttcc attagtttaa agtgtcacac aaactgagca tccatttgtt ttccttactt
     7381 ttgcccctgt attccaaggg gtttttttat aacttaaaga ttagcacacc acacaaaaac
     7441 aataaaaaaa acgacaagtt atacaataaa accagtacta tccatacagc taattacata
     7501 taaatactat taaaccatct ggacattaaa aataaactct tataacataa aattaatgcg
     7561 gttccggtac catatgtcag ggataatgtg caaatggagg tatcggaact gcatttcagt
     7621 tcattattaa ttaatgacaa aaaaaacctc tgcgagcgca gagggggtaa ggaatgttcc
     7681 cggtcataat atgtcgatag tgtttatata catacagaac aagaacaaca accagccaac
     7741 taataaatat tttatttttt agagctgaaa ccttaaagct cacttccatt ccatacattg
     7801 ttataaacct tatgttgaaa actgaaagta agtacggcag acttattaag ttgcgtgcag
     7861 tcttttcacg ttatacaaac aagaacacct gcgaagttat ttctccttac atatcccccc
     7921 tatgccaacg ggggattttt tctggttcac taacgcataa ataatagtga caccctatca
     7981 ttcacaaacc gtgataatat aaggattatc atcatggcaa aattaggtga aaacacccca
     8041 catattatcg atgctactgt agattttatg gcatcaagcc aggcatttcg tgagtatctg
     8101 aaaaaacttc cccctgataa cacaataccg ccagctctta ctgatgagca cgtaccgtta
     8161 tatataagaa ggctggaata ctatcgtctg ctttatcgac caaagcagac agaatcatat
     8221 tgaaaaaaac ggtgaacggt tcgcgaaatt ctttcgcggg tcgtgaggcg ttggacaagc
     8281 gaagcgcgtc agtttttccg gctcttcccc gtgacaggtt tctccaccag tttatcttcc
     8341 agtgtcacaa ctttctgata caccgccgct gtggcgttgt agttcctgtc gagtattgtt
     8401 ttcattcccc atccgagcac acagaacata accagaaaag ccactgacac cccacccagc
     8461 catttccagc tatacccttt aagggtgttg tattcctgca cgatattgct gtgaattgtc
     8521 gcaatatccc tgttcagggc atccgcgttt gccttcatgt ttgcgactga ctcttccagc
     8581 cgctgaaaat cctggctgat gcgctcacct atcagtcctg ccacgtccag cttctgcacc
     8641 tcatttctga cttcatccct gacctgtttt gccacaccgt cagaaatatc ccgatatccg
     8701 cgaagagttt cccttgccac agccagagaa tcagcaacag ctttctgttg ctccattgcg
     8761 gtatgttgca gatgctccac cgataacagg agcgtaagta atttttcttc tgtgctggcc
     8821 atgttcagaa tcccatagaa aatccacgtg acggtgattt aacccgttca ggtgcagttc
     8881 ggttctgtct gctcactctg tctctcatgt caaagccatg ctcccgcgca acatcatgtt
     8941 gcagccttct gaccagttca tgttgcttaa ccggagtgtc cagccattcc ctgatgtcat
     9001 gcccgaccgt tcgcgcctgt tcaagatggc gggaatagtc cggttcagac cgtttttgca
     9061 tgatgtcatg acgctgggca tcatcaagcc gtccggcagc ttcaaaaccc agatgcactt
     9121 cgggacgtcg ttcaatgccc tgttctttca gcgtccggca gtcaatgaag cccctttcaa
     9181 agccatgctt tctcatatgt tcgttgtgct tcacctccca gcgttttcgc aattcccgca
     9241 actgctcttt ttgctgagtg agagtcaggc ttccggaatc ctttttcgca cctccccgct
     9301 cgggatacct ggcgttatag cgccggaaat actgttccgg tgtacgttca atgccatcat
     9361 tcacacgctg actcatcatg atatgcgcat gaggctgttc gccgccgtca atgctcgctt
     9421 tcggattgtg aatggcaaaa ctccaggcgt gccgttcgcc agcttcctga cgtacaaaat
     9481 cctgcaccag ttcaaggcgc tgttccggtg tcagttcacg gggtaatgca atttcaagtt
     9541 ccctataggt cgaaccgttc gcacgttcaa actggtcagc cgcctgccag aaatgggaag
     9601 gattatccct ggcccattcc ggcatgtttc cgtgtgcgga atattccaaa tcctgacggt
     9661 cgcggtattt ctctttgcgc tcgatgtaat cggcgtggtt tgcagcctgc cccttgccac
     9721 caaatttaac gctgaggtga taggttgcca cggtttcagc cctctgcagg agcgcactgt
     9781 ttctcgacga aggagagaaa catataagtg cgcttccttc taattttacg aattagcacg
     9841 gaagataccc cctgcacccg ccgaccgcaa gcgatatact gcacgaccac aacacaccgc
     9901 aaaaaaacac cagggagcat cacgaacatg cgcatcagca acatcgaatg gctgaagaaa
     9961 cggatcggat tcatcaggaa gctcggagaa cagaccgcac gacagagaca gattattgac
    10021 ctgatcgaca acgaagccgg actcacagaa caggaacgca aacttctcca cgtactggca
    10081 acagcggaaa aaaacgactt acaggcacag gagagcgaac gaaaacaggc ggtccaaaaa
    10141 cgaatcgagg gaaaaaaaca acgacgggaa cgcaatcacc ggctctttct cgcggctggg
    10201 ctactgatcg aagcaggact ggtagacaca aaaacaggcg agctatgcta taaaaaagac
    10261 aggatattac aagcacttaa ggaacttaag tacgatctgg agacatcccc aaacccagac
    10321 gcgtaagtgc ctccatccgt gttccgattc cctggtgctt cctcgctcat tcgctcgcta
    10381 cgctcggtca tgagactgcg gcgagcacta ccaccacaat cacgcggaaa gccgcgccat
    10441 tgcagggctg aacggggaaa aacggcaaaa aacctgctgc tcaaaaagac gccagaaagg
    10501 cgctgttatc tgatataggc ttatttggtc tactctacga gacctcgttt tttcagttgg
    10561 taataccagg cgcgactgat ccctaatgcc tcccacggtt tcagcgtgcg tgcagatgta
    10621 gctactgtgc tgcgcttaga cttagaaccg cccttgcgac cgcgtgcagc ctgaatttct
    10681 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg gcgtgaagtt cctgtgcgtg
    10741 tatttcgcaa tgctcttgcc aatagcccga cattcagcca gtgaaagcgg aacgggaagc
    10801 gatgcattgt acatttcgac acgctggatc accgcttcaa gccactgtga gaacgctggc
    10861 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag gtggcagttt
    10921 cgccccatcc cgtaatgttt atcgatgcta cgacgctctg aggcgctcaa atcgagataa
    10981 tcagccagtt catcgagggt ataggcttcc tcgcgccact ccatcaccag ccattccagg
    11041 tggaacggat ttttgcaaat caggccgctg tagttcacat ccgcacaaag tttctcacac
    11101 agcgaacgtt caaccgctgc ggcatatttc agcgccttaa ccgaggcatc aggcgcggtt
    11161 ctcacggcga tgttgagtgc gtagagcagg tgagcgtgtc cgttcacagg atttttaacg
    11221 gtgatgttcg gcgcgggagc gttccggtcg ctccagtcaa tcgcagcccc ctcgcggtca
    11281 acgtcaaaaa ccagccagaa ctgcgcatga ggctggttct gctgtatgta ccgcgcaaga
    11341 agcgcacgcc ctttgccgga gatgcgaaga ccaaaagcga gatcatccgt gtgataggga
    11401 cgatggggta aattttcgtc gaagtattga agcgcggcgc tcatgtgtta cgattctctc
    11461 cgggttatgc gacctacacg ccctctgtat ttcatgttga atcttgccgg atctggacat
    11521 gatttcaggg gcgtttgttt ttcagtcgct tacactacac ccaacggcaa atcctgtcga
    11581 accttcccga aaacccgatc catttaccat aactcccgtt gtacgccccc cttaaaacgc
    11641 cctcagtcgc tttctggcgc aaaaaacgca aaaagacaag aaaacaccag gccaaaccag
    11701 aaaaacgcgt cagaatcgtt ttcagggcgt tgcataacta tgcacgaaag cgatgtgcga
    11761 tcggtttcgt gcgttctgaa caccgaacgg ctcaaaacga caagttttct cccctggtgc
    11821 gtggcgctct gacggggttt cctgtttcca aacttcacat ctcgccagga caaaaaaaaa
    11881 ccgacctggg gtcggtggca agaatcagag tctatgggaa atattttagt tatcggatgc
    11941 tcaatattca caaacgcaaa aaatgcctca gtgatgccct aaaacaacct ttccagaagg
    12001 agtccgcgct ttacgatgca cggaagagaa aaaaaacacc acaaaagtgg ggaaccaaga
    12061 gaaacaacaa ttacaggttg ttggttgtgt tcgaacaacg atgaagtagc tacgctaaat
    12121 aataattacc gtacaggccc tacatattca acactaaatt taagatcctg acgaaggatt
    12181 tataaatgca cgaaaacaca tagacagaca aattcactaa attcatacac aaaaacaagt
    12241 gtagttttct aagtaaatta gattcgacaa ccaattagtt tatccacatc gcattccact
    12301 cttgctacac caatcacatt tcttgatatg taatagcaga aaaaataaaa ccagacacca
    12361 atagaccaaa aaacaaacat tcaacaagaa acattttgaa caatatcaca aaactgtcac
    12421 aaccatcatc agactattga aaaaaactgg ttgctgtttt ataaaagcga ctgtaacaac
    12481 ctccactcta tagttccatt agtttaaagt gtcacacaaa ctgagcatcc atttgttttc
    12541 cttacttttg cccctgtatt ccaaggggtt tttttataac ttaaagatta gcacaccaca
    12601 caaaaacaat aaaaaaaacg acaagttata caataaaacc agtactatcc atacagctaa
    12661 ttacatataa atactattaa accatctgga cattaaaaat aaactcttat aacataaaat
    12721 taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat cggaactgca
    12781 tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag ggggtaagga
    12841 atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag aacaacaacc
    12901 agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac ttccattcca
    12961 tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact tattaagttg
    13021 cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc tccttacata
    13081 tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa tagtgacacc
    13141 ctatcattca caaaccgtga taatataagg attatcatc
//
