LOCUS       NZ_CP082044            10049 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP19-2196 plasmid pKP19-2196-3,
            complete sequence.
ACCESSION   NZ_CP082044
VERSION     NZ_CP082044.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928639
            Assembly: GCF_019823915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:38:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,032
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,719
            CDSs (with protein)               :: 5,719
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 72 of 191
            Pseudo Genes (incomplete)         :: 130 of 191
            Pseudo Genes (internal stop)      :: 19 of 191
            Pseudo Genes (multiple problems)  :: 28 of 191
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082044.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP19-2196"
                     /isolation_source="Hydrothorax"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP19-2196-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2019-02-23"
                     /collected_by="Zhengzhou University"
     gene            join(9895..10049,1..376)
                     /locus_tag="K6165_RS29830"
     CDS             join(9895..10049,1..376)
                     /locus_tag="K6165_RS29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(403..582)
                     /locus_tag="K6165_RS29835"
     CDS             complement(403..582)
                     /locus_tag="K6165_RS29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(608..1036)
                     /locus_tag="K6165_RS29840"
     CDS             complement(608..1036)
                     /locus_tag="K6165_RS29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(1200..1514)
                     /locus_tag="K6165_RS29845"
     CDS             complement(1200..1514)
                     /locus_tag="K6165_RS29845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1502..1780)
                     /locus_tag="K6165_RS29850"
     CDS             complement(1502..1780)
                     /locus_tag="K6165_RS29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(1955..2320)
                     /locus_tag="K6165_RS30215"
     CDS             complement(1955..2320)
                     /locus_tag="K6165_RS30215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(2317..2688)
                     /gene="tolA"
                     /locus_tag="K6165_RS29860"
     CDS             complement(2317..2688)
                     /gene="tolA"
                     /locus_tag="K6165_RS29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2962..3207)
                     /locus_tag="K6165_RS29865"
     CDS             complement(2962..3207)
                     /locus_tag="K6165_RS29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3534..5219
                     /locus_tag="K6165_RS29870"
     CDS             3534..5219
                     /locus_tag="K6165_RS29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5229..5486
                     /locus_tag="K6165_RS29875"
     CDS             5229..5486
                     /locus_tag="K6165_RS29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5571..5720
                     /locus_tag="K6165_RS29880"
     CDS             5571..5720
                     /locus_tag="K6165_RS29880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5903..6061)
                     /locus_tag="K6165_RS30470"
     CDS             complement(5903..6061)
                     /locus_tag="K6165_RS30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            7193..9157
                     /locus_tag="K6165_RS29885"
     CDS             7193..9157
                     /locus_tag="K6165_RS29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            9157..9888
                     /gene="mobC"
                     /locus_tag="K6165_RS29890"
     CDS             9157..9888
                     /gene="mobC"
                     /locus_tag="K6165_RS29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..10049
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5824..6391)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.795"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9158..9885
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gatgatgaaa tccatcggga tgtatgacga gcggctcaac agcgacgaaa aagccacgta
       61 ctttttcacc ggtgaaaatc cgcaatggga acgggacaaa cccacaggaa tgaagctgtg
      121 tgattacctc tatcgaaagg tggagcggtt cgccatcgag aacggtgtgc aggtggttga
      181 tgccgggaag tgctcaccgg agcagattaa cgcgatgatt gaggtggtca gcgagcggca
      241 cggggaaagc gcggtgaacg agaagctgaa agcggagctg atgcgggaat ataacagccg
      301 ctattttaac gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc ggtggtggcc
      361 gttcggcaaa aaataacgcc agcaccggca tggccggtgc tgttattctt ccccgagctt
      421 atcccgcatc acctgccaga gtgtttccgc atcctcgtga agccgttcgc acatgtcggc
      481 cggggtatcc atgtccagct cgttcagctt ttccagcaac agcagcgact gatccttaat
      541 gaatttagcc atattgaggg cagttttttc ctgcttcgtc atgttctcca ttcccttcgg
      601 taagttctca gctaaaccag ccggaccaga agcgtttttt cttctgtgtc agttctgttg
      661 tcaatctgtc cacttcggcc tggagtttcc ggcgctcatc atcgtgctgg cggcgttctt
      721 cttcccggga tgttttatct tccagcatca gggtgacagc ctgccggaga tcggccagtt
      781 ccttttgcat tgccagaagc tgttcctgtg ggaaattttt ttgtgtattt tcacggtgtg
      841 aaatgccggg aatttgtttc tcagggatat tcacatttcc gtatacacgg atcagttctg
      901 acacatccac gacagggttg tttttcccgt cacgtgacac ggtaaccttt ccctgtttta
      961 catgattgta tagcgttctt ctggtaatgc ccgcggcttg tgctgcctgt gaaaggttga
     1021 gcaatgtttt cgccactgta tacccctgat tttatcgggt gtgaaaggtg tgtaatttct
     1081 cacggtaaat gtatcgctga attttacccg tttttccgtt caggatgtgg ttttctgacg
     1141 cgctgcgctt gtcaaaccgg cgacggccac gcaatgaatt gcgcccccgt catcggttat
     1201 tagaggctgt ctgcccccgg atacccggat tctttctgat gcctgacggt gacgatcaca
     1261 atctggtcca tttcccggtc gtggcggtag agcatcacat agccactgtc gccaaacccg
     1321 atcaccagtt ccttatattc cagcggcaga aacggcaccg ggcggccaat gtctggcaat
     1381 gttttcagtt gctggatagc ccggacgatc acctcacctc cttttctggc tgccagccga
     1441 tttttggttt tgagaaagtc ctgaaggcgt tgtaaatcgc gctgtgccag cgcggaaatc
     1501 gttacctgtg gcatggcgga gcatcctgct cgttctcagt tccccaggta cttatccagg
     1561 cttccgcttc ttccgcagta aggtgcagac ccgtttcctg atagtgctgc catgcggcct
     1621 gtccgtcccg caggtactgg ctgcgcttct cttcccggtc gatgtattcg gtgattgcct
     1681 ccagcattag cgcgtgcgct gagcggtgcc ggtcatcggc cagcgttttc agccggtcct
     1741 taagttcttc atcgagcctg atggatgttg ctgatgacat aaaagcccct ttgtagtcat
     1801 atgtattaca catgactact ttaatccgtg gcggcgctat ttgccagaaa tacggctttt
     1861 gctgcccatt tttcagcttc gcttccacta atcagggctg ctacaggctc actcatcatc
     1921 caggactatt gcagcctgcg ggttaaagga cctgttactg gcggcaacca tcatcattga
     1981 gcttttgggt ggtgactggt cgcggatcct gtaagccgtc atttttcaga ctgaattcaa
     2041 aatttataac ctgtcgcatt gccgggtgcc ctgcgggata tagccagtgc ctcattgccg
     2101 tcagcgtttc ccgaaaaaaa aggtgacagc cggtaagcga cacgttgtcc actcgtccat
     2161 cgtccagaat atcaaacgtg gcggtcacct gaccttcgat gcgttttgct gcggcctgta
     2221 ccgggtaggt tggctgcagt gtggttacgg gttttagtgg tatcgcatgt accggagtga
     2281 ggaacatggc cgccagcact gtcagagtaa aagctttcat ggtctgaaat ccagaggggc
     2341 gtttttgaat atttgccagg tattttcatc cggtgccggc ggaattttgg cgtgggtaac
     2401 ggctgatatt gctgctttac agagtttcgg atctcctcct tcagcaatgg cgctgttcag
     2461 agtgccatca cgttgtatgg atatccgcac ggtgcacatt tttcccgcat aggcctctgg
     2521 ttcaaacatc tgcatttcaa cagcggtctt gatggccgca gcgtagctac tgattttaga
     2581 tgacttatcc agagtataaa ttttcggcgg ctgggtggtg gaatgcttac aggccgtcag
     2641 gagtaagggg ctgcacaatg ccagcaggat aaagggtatt aaacgcatcg ggttgatgac
     2701 ctgtcagtaa aaaatgttac atgtatattt gtcactcttt acggccacgt tcatttacat
     2761 aaacacggct tatcgtatcg tttttacacc aaacggcccg ccatgcagtt gcgtgcatag
     2821 gcatgcagcg ggttacaatt taccgggcgc gtttccggcg gttttccggg tggtttgttg
     2881 cctgttttat cccgtagccg ccggaaacgc cctcagaccg tctgagcggt gcgggcaatg
     2941 cgacgttatg gtaaataccc cttaccggcg gcgctgtggc tgccaggtga aggtggtttc
     3001 cgtagcctcg tatttctccc gacgcatttc ctgcgcccgc ctggcttcac gttcgtcctg
     3061 aatggcacga agcacccttt cccgcacctg ttgttttacc gcctttggca gcgttttgtt
     3121 ccctttcagg cccatggcca gtgcctgagc gacaaaggcg tccgccaggc gcttttcttc
     3181 agcgggagtc agtttcaggt gcgtcatagt ggcctccggg cgtctcaggt ttcccgaaca
     3241 gtttaaccgc aggtcctctc ctcatgccag ttagctttca gatgtgtcta taccatctga
     3301 aatgtaagtg tgatgaatgt cttaaataat aaaatttgtt gccatgagaa attttcggga
     3361 aaagacgcga tctgcttaca ggtggctccg ggcgcttttt tgtgcctgaa gatgagaact
     3421 aatctcatat attgacacct gaaaacggga ggagtaaggt aataattata ctgtgtatat
     3481 gtacagtatg ttggctttaa tttattgttt taaatgtcaa agaggaaaac ggtatgagtg
     3541 gtggagacgg tcgaggtccg ggtaattcag gtctgggaca taatggtggt caggccagtg
     3601 ggaatgtgaa cggtacgtct ggtaaaggtg gcccttcatc aggtggtggt acggatccaa
     3661 acagcgggcc gggctggggt acgacgcata cgccgaatgg agatattcat aactataatc
     3721 cgggggagtt tggtaacggc gggagtaaac ccggtggtaa tggtggtaac agcggcaatc
     3781 atagcggtag ctctggtggc ggacagtctt cggccactgc gatggccttc ggtctgcctg
     3841 ccctggctac tccgggggct gaaggactgg ctttatcagt ttccggcgat gcgttgtcgg
     3901 ccgccgttgc tgatgtgctg gctgccctga aggggccgtt taagtttggt ctgtggggga
     3961 ttgcgatcta cggtgtgctg ccttctgaga ttgcaaaaga tgatccgaaa atgatgtcaa
     4021 aaattatgac gtcattaccg gccgatgcgg tgacggagac tccggcaagt actttaccac
     4081 tggaccaggc gacggttcgt gtcagacaac gggttgtgga tgtggtgaag gatgagcggc
     4141 agcatattgc ggttgtcgca ggtcggccaa tgagtgtccc tgtggtggat gcgaaaccga
     4201 caaaacgtcc gggggtattc agtgtgtcga ttccgggtct cccgtctctg caggtgagcg
     4261 taccgaaagg tgttccgaca gcgaaagccc cgccaaaagg cattgttgct gaaaaaggtg
     4321 attcacgtcc ggctggtttt acagccggtg gtaactcccg tgaggccgtt attcgtttcc
     4381 cgaaagagac cggacagaag ccggtttatg tgtcggtgac agatgttctt accccggcac
     4441 aggtaaaaca gcgtcaggag gaagaaaagc gtcgccagca ggcatgggac gccgctcatc
     4501 cggaagaggg gctgaaaaga gactatgata aagcgaaagc tgagctggat gccgaagata
     4561 aaaatattgc gaccttaaac agccgcattg catcgacaga gaaggcgctc cccggtgcaa
     4621 gggctgctgt tcaggaagcc gataaaaagg taaaagaggc agaggcaaat aaagatgatt
     4681 ttgtgactta taacccgcct catgaatatg gctccgggtg gcaggatcag gttcgctatc
     4741 ttgataagga tattcagaat cagaatgaga aattaaaagc ggctcaggca tctttaaacg
     4801 caatgaatga atccttatcc agagataagg ctgcacttac cggggcgatg gagagccgga
     4861 aacaaaagga gaaaaaagcg aaggatgcag aaaataagtt aaatgaggaa aagaacaagc
     4921 ctcgcaaggg gactaaagac tacggccatg attatcatcc agtacctaaa acagaagaca
     4981 taaagggact tggagagtta aagcgaggtg atcctaaaac acctaaacaa ggtggtggtg
     5041 gtaagcgggc ccgatggtat ggcgataaaa aacgtaaaat ttatgaatgg gattcccagc
     5101 acggtgagct tgaagggtat cgtgccagtg atggcgaaca cctaggcgca tttgatccca
     5161 aaacgggtaa gcaggttaaa gggccagatc caaaacggaa cattaaaaaa tatctttgag
     5221 aggtaaatat ggggcttaaa ttacgtttga catggtttga taagaaaact gaagagttta
     5281 aaggggaaga gtattctaaa gactttggtg acgatggttc tgtcattgaa agcttgggaa
     5341 tgcctttaaa ggataatatt aataatggtg agtttgattt gaagaaagaa tgggttcctt
     5401 tactacagcc attttttaaa aatcaaataa aacctgatgt ggataattgt tatatagctt
     5461 ttgattatcg ggacggtaac tggtaacaaa aaattatcaa ggatgatttg aaatactgac
     5521 ttccggtgtt cagggatgaa tgccggagtc tttaaataca gggaaagtat atgaaaaagg
     5581 taaagactat ttttcttttt atattgattg ttgctggctt tcttctggcc gcatgtcagg
     5641 caaattatat ccgggatgtt cagggcggaa cggtggcacc atcgtcctcc tctgaactga
     5701 cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5761 cgtaatctct tgcactgtaa acgaaaaaac cgcctgggga ggcggtttga tcgaaggtta
     5821 agtcagttgg ggaactgctt aaccgggtaa ctgggtatac gagaccgcag ataccaaaac
     5881 tgttctttca gtgtagccgc agttaggcac ccacttcaag acctctcaat atctctctcg
     5941 tatatatcca gttaccaatg gctgctgcca gtggcgtttt tgcgtgtccg tccgggttgg
     6001 actcaagatg atagttaccg gaaagggcgc agcagtcggg ctgaacgggg ggttcgtgca
     6061 tacagcccag cttggagcga acgacctaaa ccgagccgag ataccagcag cgtgagctat
     6121 gagaaagcgc cacgcttccc gaaagggaaa aaggcggaac aggtatccgg taaacggcag
     6181 ggtcggaaca ggagagcgca agagggagcg acctaccgga aacggtgggg atctttaagt
     6241 cctgtcgggt ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg
     6301 caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg gcggagccta tggaaaaacg
     6361 ccaccggcgc ggccctgccg ttttgcctca catgttagtc ccctgcttat ccacggaatc
     6421 tgtgggtaac tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg
     6481 accgagtgag cgaggaggcg caatttcccc ggcacctgca agacggtcac cggacgcgct
     6541 gatgtgggat attgtcccct gattaagtct ttctattccc tgacgatcaa ttatcaggat
     6601 aggtgaacca tgtcggtttt tcgctgaaca ttttacgtta acctatctct gttgcttttt
     6661 tctccttgcg gagaaatgca agtcatcccc gctggggcgt ggctgacttc aggggaaaag
     6721 accttgctta cgctggctgg acgccaccga cgcacggtct tttcccctct ttctggctct
     6781 gtccggctgg acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga
     6841 ccacccacaa aaatccacgg tgctggacgc acctgacgaa gcagcaaagc tgctcctgat
     6901 tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa
     6961 gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg
     7021 gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc tccgcggtga aagctgaacg
     7081 caatcaccac ggacgtgacc gccgccgcca tgccttgggg catgtccgcg gcttgtgcag
     7141 cgggggctgc gcatcacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa
     7201 tacggattcg cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg
     7261 ggtcagctgg ctgtaccggg agtattacag tctggccggt ttttttctgg ttatcggcct
     7321 gtttttcggt ctggctgaac tctgggccgg ccgaatgcgt tttgtggggc tgagccataa
     7381 ggcgcggcgg gagtatgccg atcgctataa gcgcgataat aaccggactg tgccattttc
     7441 ggtgctggcc tcgctggatt cacccgggcg gatggtcagg gcacggcgac atgtctggaa
     7501 ctacgcgtac tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc
     7561 cgtgtttatc gagagctggg gagaaaccgt cctgcgcccg gacctctatg cgaacgcaga
     7621 ttattacctg ctgcagccgg ctaacctgtt cgggatagcc tggtgtgccg gagggcttgt
     7681 cgccggcgtc cttctggcgc ggctgctggc gttcctgctg cttgatggtc attttgcggc
     7741 cgctgacgag gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac
     7801 cggagaaatg acagacgtcc gccacctgca tttcggggag ccggtaccgg ttaacgcgct
     7861 ggccgatttt tccacggagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga
     7921 gcagggccag ccggtgctgg tccctcgtga tacctggcgc aagaccaata ttcagatcct
     7981 cggcctgccc gggagcggca agagcgcgat ggccaccaac gcgctcattc gctgcgtacg
     8041 cgatttcggg gacgctgtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt
     8101 cttccgggcg cactgcccgg acttcaccct gctggatctg cggccgggga aaccggcgca
     8161 gctgaatctg ttccgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt
     8221 taacctgagt gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc
     8281 gaagctgatt gcggagcagt tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc
     8341 gtacgcgttg ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa
     8401 cgtggccgac ctgagcgtcc tgcagacgga cagcggtatc gatgtggcgg ggataatcaa
     8461 cggcggcggc tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca
     8521 gaagatgctg tttgcccgct gcgcgcaaat catcattgcg cgggatgagt tccggaggtg
     8581 gccgcacgcg agcatcatgc tcgatgagat taaatatctg ctgtcgaagt acgtgctgaa
     8641 cgcgctgggg acgctgcgca gccgggactg caacctgctg ctcgcgcacc agtcgctggg
     8701 cgacttcggt cagtgcgggc aggatctgcc cgctgatttt gtgaaaacca cggtactgga
     8761 taacacgccg atccgctggt tctatcgcgc ggccagccag gagtccgcgc agtgggcggc
     8821 gggccagacc ggggagatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg
     8881 gaacgtggag cacatcagcg gcgacagctt catccagaag gaggcgcggc cgctgtttga
     8941 cgtgaacacc ctgcaacacc tgcctgacgg gttcgcggtg atgaccgggc tgggcgtggc
     9001 gcggctggcg ttcagcagcc cgctgcgggt tgagcgccgg gaaatttcgc tgaaaacctt
     9061 cccggtgctg gcgaaaacgg acccgctggc ggagtacctg cctccggccg acggcggcgg
     9121 accaaaagcg gatgatgatt ttgcggggct ttactgatga cactggagca gtacaacgtc
     9181 agccacgcga agcggcaggc gcggaacgcg gagaaaacac ggctgaccct gcgctggctg
     9241 cgggaagagc tgtgttccac ggcggagctg gttgcgcgac ggctggggat tgcggcggta
     9301 cagccggttt accgcttcct ggacagtctg gtggcgaaag ggctgctggt ccgggcgaaa
     9361 tacccggttg acggccgtca ggtcagcgtg tgggggctga cgccgcacgg ggtggcgttc
     9421 tcgttcgacg aggacgagcc gctgacggac gttattccgt ttcagccgtc aagggtgtcg
     9481 gcggcgcagc tgccgcaccg gctggccgtg cagtccctgc gcctctccat ggagggccgg
     9541 ggggccaccg gctggcggta cctgcaccgt atggccctga aagggatgaa agtgccggac
     9601 gcgctggcgg agctggacgg cagaacggtg gcatttgagg tagagcgcac cgtcaagagc
     9661 cgcaggcggt atcaggaagt ggtgtccggg tatctgttca accgccgggc gaacggcatc
     9721 gatgaaatct ggtacatctg ccctgaccgg ccgacgcttg tccgggtaca gcgggcgatt
     9781 ctggcggtgg atgaaatcgt gaatccacag accggcgagg cccgcaaaac ggtggagctg
     9841 gaccgggaga ggctgttcgc gtgctttaaa tttatgacaa cggagtgagg gattatggcg
     9901 ctgtacatgg tatttattga ggacgtggaa gacaaagacg gcgagcgctg cattatgcca
     9961 accagcgagc atctggaaga catggtggtg ctcgcctggc agaccggggc gtatgttttc
    10021 tgttttgtcg accgggccca gcatctggc
//
