LOCUS       NZ_CP082040             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP19-2196 plasmid pKP19-2196-4,
            complete sequence.
ACCESSION   NZ_CP082040
VERSION     NZ_CP082040.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928639
            Assembly: GCF_019823915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:38:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,032
            CDSs (total)                      :: 5,910
            Genes (coding)                    :: 5,719
            CDSs (with protein)               :: 5,719
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 72 of 191
            Pseudo Genes (incomplete)         :: 130 of 191
            Pseudo Genes (internal stop)      :: 19 of 191
            Pseudo Genes (multiple problems)  :: 28 of 191
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP19-2196"
                     /isolation_source="Hydrothorax"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP19-2196-4"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2019-02-23"
                     /collected_by="Zhengzhou University"
     gene            join(5568..5596,1..238)
                     /locus_tag="K6165_RS00005"
     CDS             join(5568..5596,1..238)
                     /locus_tag="K6165_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(308..658)
                     /locus_tag="K6165_RS00010"
     CDS             complement(308..658)
                     /locus_tag="K6165_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(724..1269)
                     /locus_tag="K6165_RS00015"
     CDS             complement(724..1269)
                     /locus_tag="K6165_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(1295..1621)
                     /locus_tag="K6165_RS00020"
     CDS             complement(1295..1621)
                     /locus_tag="K6165_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1979..2137)
                     /locus_tag="K6165_RS30325"
     CDS             complement(1979..2137)
                     /locus_tag="K6165_RS30325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2777..2953
                     /locus_tag="K6165_RS00025"
     CDS             2777..2953
                     /locus_tag="K6165_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2943..3287
                     /locus_tag="K6165_RS00030"
     CDS             2943..3287
                     /locus_tag="K6165_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3496..>3930
                     /locus_tag="K6165_RS30220"
                     /pseudo=""
     CDS             3496..>3930
                     /locus_tag="K6165_RS30220"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3965..4474
                     /locus_tag="K6165_RS00040"
     CDS             3965..4474
                     /locus_tag="K6165_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4481..4693
                     /locus_tag="K6165_RS00045"
     CDS             4481..4693
                     /locus_tag="K6165_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5069..5197)
                     /locus_tag="K6165_RS30225"
     CDS             complement(5069..5197)
                     /locus_tag="K6165_RS30225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            5360..5584
                     /locus_tag="K6165_RS00050"
     CDS             5360..5584
                     /locus_tag="K6165_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     Typing          2612..2691
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2029..2426)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3278..4809
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgctcaaa tcgttacgca agatggacaa acagaacgca cgacggattg tggattttat
       61 gagtttacgc attgcagttg ctgccgatcc tcgccagtca gggaagccgc tcaaaggtga
      121 gctgggcgag ttctggcgct atcgggtggg agattatcgc gttctgtgtg agatccgaga
      181 tgacgagctt gttatccttg ccgccacgat tggacatcgc cgcgaagttt acgactgaag
      241 cccgctatgc gggctttttt gatgccgaca acaggtatta tggtaaatac tcgactaact
      301 acgtgttcta ctgtttgtcg gttttagtta accagcaggt tgtcttaacg ataatgtagc
      361 cgttattttt aactaataag gtcatattgt taacgtcagg cataataaaa gacattatgg
      421 cctcagagtt atcatcagaa gcagctctct gcattactac ttcgattccc ttttctttga
      481 atctataagt atccagttgc ctattaaact tagaaagacc tacaaccata cttagtttgt
      541 tatctttaac agtaagagat gcttcctgag taccattaca tcggtagtaa gcggtgtaac
      601 cgtcagcaaa agatcgaccg gggaatataa atgctaggaa cgcaattaac caatacatag
      661 ttgagaatct tggtattaca catttcacta tcctctcttt catttttcgt tcaccatact
      721 ttgttatctg atttttaatt tttctatttt ataaaccaag tctctaccaa aaaggcgaaa
      781 attatcctca aataataata attgtttaag aacatccatg aaagcagctg cttcaccctc
      841 agttatggga gctactctat ataacatata acctgagtta agggtttctt ttatactttc
      901 ttcaatgctc attataaata ttagtggatt atcatttttc ttcccccatg ggtttctaat
      961 taatccttcg atgtactcag gtcctttgcg tcctattgcc tgcgcaatat tttttatttc
     1021 atctgcgtga gggctttgtt gtacagcttt gtttatgcct gcttgagtga ttttcagaga
     1081 ctctacaccg tgtatgttgt atgagataaa atgtaataaa ttaagatgca atgcaattaa
     1141 tccatttttg tttacatctg agtatttgga taaaaaacgt aatgaagtgt taactctccc
     1201 ttgaagccta acaattggga gtgctgcctt gtatatttcc ttctccgtta aataccgttt
     1261 aaaaaacata attctccgcc tccgtatagc gtgcctattt tatttcgtcg taaaacttag
     1321 ctctgaaata ttcctttaga accatcatgt cattaataat tgaatctatc tcatccattt
     1381 tttcttggta ttcttttaat ggtgaaggga gcggtaactc aatatttgaa aagtgattgg
     1441 cgagataggg atcaaggttt ctcatccatt caggcttttc gccaatgcgt tcttcaactg
     1501 aaagtgaacc tgataatctc tggttaataa gatcacttag actcattttg gttttattcg
     1561 aaagccgttg taaggattgc tttaatccta tatctagcat gatatttgtt ctttctctca
     1621 ttaccccccc ttgctattgc gttttttgtt gcgcacaaag ctaccattgc gcattaagaa
     1681 acgcaataaa aaaggaggga gggagtggtt tgtacatgat gtgcagtgcg ctgagggcat
     1741 cagtggcggg tgtttttccg tgggatggat ggagctccgg ggataacaga actgacgatc
     1801 atttaaagga tcttcttgag atcctttttt tctgcgcgta atctcttgca ctgtaaacga
     1861 aaaaaccgcc tggggaggcg gtttgatcga aggttaagtc agttggggaa ctgcttaacc
     1921 gggtaactgg gtatacgaga ccgcagatac caaaactgtt ctttcagtgt agccgcagtt
     1981 aggcacccac ttcaagacct ctcaatatct ctctcgtata tatccagtta ccaatggctg
     2041 ctgccagtgg cgtttttgcg tgtccgtccg ggttggactc aagatgatag ttaccggaaa
     2101 gggcgcagca gtcgggctga acggggggtt cgtgcataca gcccagcttg gagcgaacga
     2161 cctaaaccga gccgagatac cagcagcgtg agctatgaga aagcgccacg cttcccgaaa
     2221 gggaaaaagg cggaacaggt atccggtaaa cggcagggtc ggaacaggag agcgcacgag
     2281 ggagccgccg gagagaaatc accggcatct ttaagtcctg tcgggtttcg tcctcctctg
     2341 acttgagcgt ccatttctgt gatgctcgtc aggggaggcg gagcctatgg aaaaacggca
     2401 acgccgcatc cttttctccg gccctttctg atttgagcgc ctttgcttta tcccgttatc
     2461 cgtgagtcgg tcacgcccgc cgcagccgaa cggcaggagc gcagctaccg agtgagcgag
     2521 gaggcgtcat ttcatctgtc cgtgcatggt attgcggacg ctcctgagcc acatatcaca
     2581 cctgccacat gaggcacttt catgaatcgt gctctcatgc caacatagta agccagtata
     2641 cacagcgtaa acgctgtgac tggttcaggg ctgcgccccg aaacccgcta acaccactga
     2701 cgcgctggcg cttgtccaac gccggagcga cccggaaaac ttttccgtgc cgtccggcgt
     2761 ttgcgaagga gggataatgc agaagccgaa gaagaagccg ctcgtcgtaa aaaaaagatc
     2821 ctggcatccg gcagtgatgg caaatctgat gcgtcaggca caggaggaag attaccgccg
     2881 ggagcagatg gcagagcttg agcgttcagc ccggtcacat tttgcggata tcggaggaga
     2941 ggatggcggt taagcgtacc cgttttttag gtatccgggt gaccgatggg gagtaccagc
     3001 agctgctgga gcgctgcaat ggaaggcaac tggcggtgtg gatgcgggaa acctgtctcg
     3061 atacgcgccc ggcgcggtcg ttgcggctgc cgtccatcga tccggttttg ctgcgccagc
     3121 ttgccgggat ggggaacaac ctcaaccaga tcgcccggaa aattaacggc ggccagtggt
     3181 caggcgctga ccgggttcag gtggtggccg cgctgatggc cattgatgcc gaactcgagc
     3241 ggctgcggca tgccgtgctg gaaaaggggg cagacgatga tcgttaagtt tcatccgcga
     3301 gggcgaggcg gcggtgccgg gccggtggat tatctgctgg ggaaagaccg ccagcgcgac
     3361 ggggccatcg tcctgcaggg aaagccggag gaggtccggg agcttatcga cgcctcgccc
     3421 tacgccaaaa agtacacctc cggcgtcctg tcttttgctg aacaggattt accgcccggc
     3481 cagcgtgaaa agctgatggc gagcttcgag cgggttctga tgcccggact cgataaagac
     3541 cagtacagcg tgctgtgggt tgaacaccgg gacaaggggc ggctggaact gaattttctg
     3601 gtaccgaaca cggaactgct gaccggcagg cgtctccagc cgtactacga ccgggcagac
     3661 cgtccgcgca tcgatgcgtg gcagaccgtg gtgaacgggc gactggggct gcacgacccg
     3721 aacgcaccgg agaaccggcg cgcgctggtg acgccatctt cgttacccaa aacgaagcag
     3781 gaagccgcag aggctattac gcggggctta ctggccctcg cctcgtcagg ggagcttaaa
     3841 acgcgtcagg acgtcactga ggcgctggaa agcgcaggtt ttgaggtcgt gcgcaccaca
     3901 aagagcagca tcagcattgc cgacccggac ggagggcgaa acatccgact taagggagcc
     3961 atctatgaac agtcttttaa cgctggcgaa ggacttagag cagaaatcga aagcgcagca
     4021 gcagagtacc ggcgaaatgc tgaaagccgc attcagcgag cacgagaagt ctgtcagagc
     4081 ggaactgagc gaaagcggga agagaatcag cgccgccatc cgcgcccacg agcagggtat
     4141 gagcgcggcc atgcagtcga accgcctgag cgtgatgcgt atggtcgggc ggacatggct
     4201 gaccatcacg atggtgtcag gactgctgtt cgccagcctg agcggggtgt tgtggtatca
     4261 ggggagcttg atagcgtcaa atctggccga aatcgaccgg cagaacgcag cgctgtcgaa
     4321 gctgaacgcg aagacctggg gcgtgacgta cctggaagac agcaacggac gttttctggt
     4381 gctgccgaag gggacggccg tagacaggac gcagagctgg acggtcggga acgggcggtc
     4441 gaagcagaac gcgctgagac tggtgaagga gtaagtgccg atgaccgagc tggaaaaaca
     4501 gttgctgagc gcattagagc agctacagca ggactactcg aaaaggctgg acgagtggga
     4561 gagcgccttc ggggaatggc ggaaaatgtc agggcttatg caacgggaga acgcggcgct
     4621 gagcgagcgc gtgacgcgct tgagtcagca ggtggaacgc ttgagcgggc aactgcagcg
     4681 tttgagccgg tagtgcagcg tcatgagatg gccgtggccg cagaacgggc gcacgagcaa
     4741 cggcagcacg aaaaggaact gacggaagcg cgttcccggc agcgaagcta tgacggtccg
     4801 tcgctgggct gaaatgcagg aaaacagggt tctcatcggg catttacgct gaaaccggat
     4861 gtcattcacc tggctgcatg gctatgcagc caggtaaaat ttatctgtcg cgaacggggc
     4921 ggtttatcgg gtggtttgtt gccggtttta cctgactgcc accccgttcg cggcgaaccc
     4981 gtccggggcg gtgcgggcaa cgggtgttat gtttaatcag cgtgaacggc agatacaaaa
     5041 aagccccgga gggcttttaa ctactgactt atttttttga agatttgctg ggtttcaatt
     5101 tgtcagtatt tcccggcgtt tttttattgt cacgctggcg tttatcaggt tcccctgttg
     5161 aatcggcaat tcgtttcgga cccggcttaa gacccataat cgttatcctt tcaatagtta
     5221 agaggaaagc cctcaagcta gatatattga ccgcgttacg gattatcaag aggggctaac
     5281 aagattattt tttgttcttt taggttgatt gtggtactgt tgatatacat gtatagcaaa
     5341 tggtgttcgg gaggtttata tgcttgctat ccggttatct gatgagattg agtcccgtct
     5401 ggactcgctg gcgaagcaaa ccggcagaac aaagacgttt tatgcgcggg aagcaatact
     5461 ggcgcatctg gaagacctgg aggattatta tctttcagca gaaactgctg cacgcgttcg
     5521 ccgtggtgat gaagcagtgc attcgtctga agacgtgagg aagtcacttg gtctggacga
     5581 ttaactattc cgatcg
//
