LOCUS       NZ_CP082038            10049 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2172 plasmid pKP18-2172-2,
            complete sequence.
ACCESSION   NZ_CP082038
VERSION     NZ_CP082038.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928638
            Assembly: GCF_019823955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,635
            Genes (coding)                    :: 5,477
            CDSs (with protein)               :: 5,477
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 58 of 158
            Pseudo Genes (incomplete)         :: 107 of 158
            Pseudo Genes (internal stop)      :: 15 of 158
            Pseudo Genes (multiple problems)  :: 22 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082038.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2172"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2172-2"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-12-24"
                     /collected_by="Zhengzhou University"
     gene            complement(join(9974..10049,1..455))
                     /locus_tag="K6161_RS27505"
     CDS             complement(join(9974..10049,1..455))
                     /locus_tag="K6161_RS27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(462..1193)
                     /gene="mobC"
                     /locus_tag="K6161_RS27510"
     CDS             complement(462..1193)
                     /gene="mobC"
                     /locus_tag="K6161_RS27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1193..3157)
                     /locus_tag="K6161_RS27515"
     CDS             complement(1193..3157)
                     /locus_tag="K6161_RS27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4289..4447
                     /locus_tag="K6161_RS29010"
     CDS             4289..4447
                     /locus_tag="K6161_RS29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            complement(4630..4779)
                     /locus_tag="K6161_RS27520"
     CDS             complement(4630..4779)
                     /locus_tag="K6161_RS27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4864..5121)
                     /locus_tag="K6161_RS27525"
     CDS             complement(4864..5121)
                     /locus_tag="K6161_RS27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5131..6816)
                     /locus_tag="K6161_RS27530"
     CDS             complement(5131..6816)
                     /locus_tag="K6161_RS27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7143..7388
                     /locus_tag="K6161_RS27535"
     CDS             7143..7388
                     /locus_tag="K6161_RS27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7662..8033
                     /gene="tolA"
                     /locus_tag="K6161_RS27540"
     CDS             7662..8033
                     /gene="tolA"
                     /locus_tag="K6161_RS27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8030..8395
                     /locus_tag="K6161_RS28775"
     CDS             8030..8395
                     /locus_tag="K6161_RS28775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            8570..8848
                     /locus_tag="K6161_RS27550"
     CDS             8570..8848
                     /locus_tag="K6161_RS27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            8836..9150
                     /locus_tag="K6161_RS27555"
     CDS             8836..9150
                     /locus_tag="K6161_RS27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            9314..9742
                     /locus_tag="K6161_RS27560"
     CDS             9314..9742
                     /locus_tag="K6161_RS27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            9768..9947
                     /locus_tag="K6161_RS27565"
     CDS             9768..9947
                     /locus_tag="K6161_RS27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             131..10049
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3960..4527
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.795"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(466..1193)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cggctgttat attcccgcat cagctccgct ttcagcttct cgttcaccgc gctttccccg
       61 tgccgctcgc tgaccacctc aatcatcgcg ttaatctgct ccggtgagca cttcccggca
      121 tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca cctttcgata gaggtaatca
      181 cacagcttca ttcctgtggg tttgtcccgt tcccattgcg gattttcacc ggtgaaaaag
      241 tacgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga tttcatcatc
      301 gccagatgct gggcccggtc gacaaaacag aaaacatacg ccccggtctg ccaggcgagc
      361 accaccatgt cttccagatg ctcgctggtt ggcataatgc agcgctcgcc gtctttgtct
      421 tccacgtcct caataaatac catgtacagc gccataatcc ctcactccgt tgtcataaat
      481 ttaaagcacg cgaacagcct ctcccggtcc agctccaccg ttttgcgggc ctcgccggtc
      541 tgtggattca cgatttcatc caccgccaga atcgcccgct gtacccggac aagcgtcggc
      601 cggtcagggc agatgtacca gatttcatcg atgccgttcg cccggcggtt gaacagatac
      661 ccggacacca cttcctgata ccgcctgcgg ctcttgacgg tgcgctctac ctcaaatgcc
      721 accgttctgc cgtccagctc cgccagcgcg tccggcactt tcatcccttt cagggccata
      781 cggtgcaggt accgccagcc ggtggccccc cggccctcca tggagaggcg cagggactgc
      841 acggccagcc ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa cggaataacg
      901 tccgtcagcg gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt cagcccccac
      961 acgctgacct gacggccgtc aaccgggtat ttcgcccgga ccagcagccc tttcgccacc
     1021 agactgtcca ggaagcggta aaccggctgt accgccgcaa tccccagccg tcgcgcaacc
     1081 agctccgccg tggaacacag ctcttcccgc agccagcgca gggtcagccg tgttttctcc
     1141 gcgttccgcg cctgccgctt cgcgtggctg acgttgtact gctccagtgt catcagtaaa
     1201 gccccgcaaa atcatcatcc gcttttggtc cgccgccgtc ggccggaggc aggtactccg
     1261 ccagcgggtc cgttttcgcc agcaccggga aggttttcag cgaaatttcc cggcgctcaa
     1321 cccgcagcgg gctgctgaac gccagccgcg ccacgcccag cccggtcatc accgcgaacc
     1381 cgtcaggcag gtgttgcagg gtgttcacgt caaacagcgg ccgcgcctcc ttctggatga
     1441 agctgtcgcc gctgatgtgc tccacgttcc ccgcctcccg gctggcccgg cggcgctcca
     1501 cgtccacccg gatctccccg gtctggcccg ccgcccactg cgcggactcc tggctggccg
     1561 cgcgatagaa ccagcggatc ggcgtgttat ccagtaccgt ggttttcaca aaatcagcgg
     1621 gcagatcctg cccgcactga ccgaagtcgc ccagcgactg gtgcgcgagc agcaggttgc
     1681 agtcccggct gcgcagcgtc cccagcgcgt tcagcacgta cttcgacagc agatatttaa
     1741 tctcatcgag catgatgctc gcgtgcggcc acctccggaa ctcatcccgc gcaatgatga
     1801 tttgcgcgca gcgggcaaac agcatcttct gcacccggat caccgcctca tcgtccatcg
     1861 agccgatgac gtacaggcag ccgccgccgt tgattatccc cgccacatcg ataccgctgt
     1921 ccgtctgcag gacgctcagg tcggccacgt tctccagctt ggtgatcagc cccttcacgt
     1981 cctttttcag ggcctccggc aacgcgtacg ccgccgccag cacctgctga atgtttgccc
     2041 cctgcgggaa ctgctccgca atcagcttcg ccgctttctg ctcgctgatg cggtagtggt
     2101 ccgccacgtc gccggtctca ctcaggttaa acccggcaac cagcagattc ttcagggcgt
     2161 actggtcgag atcgcggaac agattcagct gcgccggttt ccccggccgc agatccagca
     2221 gggtgaagtc cgggcagtgc gcccggaaga cgtgcggcgc ccacgcgtcc cctttggggt
     2281 cgaagtacac cacagcgtcc ccgaaatcgc gtacgcagcg aatgagcgcg ttggtggcca
     2341 tcgcgctctt gccgctcccg ggcaggccga ggatctgaat attggtcttg cgccaggtat
     2401 cacgagggac cagcaccggc tggccctgct catccttacc cagaaacacc gcctgctgtt
     2461 tccgggcctg ctccgtggaa aaatcggcca gcgcgttaac cggtaccggc tccccgaaat
     2521 gcaggtggcg gacgtctgtc atttctccgg tccgctggct gctgcgccgg ctctcctgat
     2581 tcagtttcgc gttcaccgcc tcgtcagcgg ccgcaaaatg accatcaagc agcaggaacg
     2641 ccagcagccg cgccagaagg acgccggcga caagccctcc ggcacaccag gctatcccga
     2701 acaggttagc cggctgcagc aggtaataat ctgcgttcgc atagaggtcc gggcgcagga
     2761 cggtttctcc ccagctctcg ataaacacgg cgacaaccag cccccccaga agggccagac
     2821 cggcagcgcc ggtcagccag tacgcgtagt tccagacatg tcgccgtgcc ctgaccatcc
     2881 gcccgggtga atccagcgag gccagcaccg aaaatggcac agtccggtta ttatcgcgct
     2941 tatagcgatc ggcatactcc cgccgcgcct tatggctcag ccccacaaaa cgcattcggc
     3001 cggcccagag ttcagccaga ccgaaaaaca ggccgataac cagaaaaaaa ccggccagac
     3061 tgtaatactc ccggtacagc cagctgaccc acgggtaaat ctgccgggcc agctccggga
     3121 tatgccactg ccaggcaagc gaatccgtat taaacatgca atctctctcc ttcagacgac
     3181 gggtttaccg gcgtgatgcg cagcccccgc tgcacaagcc gcggacatgc cccaaggcat
     3241 ggcggcggcg gtcacgtccg tggtgattgc gttcagcttt caccgcggag gccagcctct
     3301 ttccgcccca tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg
     3361 agaggaaaag gcggcacccc agtgcggtct tttccggtgt tccggccccc gtcagagttc
     3421 gccatagcga gcggctgacc acccgcaaaa tcaggagcag ctttgctgct tcgtcaggtg
     3481 cgtccagcac cgtggatttt tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa
     3541 tttctggcgt ggccggcgtc cagccggaca gagccagaaa gaggggaaaa gaccgtgcgt
     3601 cggtggcgtc cagccagcgt aagcaaggtc ttttcccctg aagtcagcca cgccccagcg
     3661 gggatgactt gcatttctcc gcaaggagaa aaaagcaaca gagataggtt aacgtaaaat
     3721 gttcagcgaa aaaccgacat ggttcaccta tcctgataat tgatcgtcag ggaatagaaa
     3781 gacttaatca ggggacaata tcccacatca gcgcgtccgg tgaccgtctt gcaggtgccg
     3841 gggaaattgc gcctcctcgc tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg
     3901 cgctgcggac acatacaaag ttacccacag attccgtgga taagcagggg actaacatgt
     3961 gaggcaaaac ggcagggccg cgccggtggc gtttttccat aggctccgcc ctcctgccag
     4021 agttcacata aacagacgct ttttcggtgc atctgtggga gccgtgaggc tcaaccatga
     4081 atctgacagt acgggcgaaa cccgacagga cttaaagatc cccaccgttt ccggtaggtc
     4141 gctccctctt gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt
     4201 ttccctttcg ggaagcgtgg cgctttctca tagctcacgc tgctggtatc tcggctcggt
     4261 ttaggtcgtt cgctccaagc tgggctgtat gcacgaaccc cccgttcagc ccgactgctg
     4321 cgccctttcc ggtaactatc atcttgagtc caacccggac ggacacgcaa aaacgccact
     4381 ggcagcagcc attggtaact ggatatatac gagagagata ttgagaggtc ttgaagtggg
     4441 tgcctaactg cggctacact gaaagaacag ttttggtatc tgcggtctcg tatacccagt
     4501 tacccggtta agcagttccc caactgactt aaccttcgat caaaccgcct ccccaggcgg
     4561 ttttttcgtt tacagtgcaa gagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4621 tgatcttttc tactgaaccg cgatccccgt cagttcagag gaggacgatg gtgccaccgt
     4681 tccgccctga acatcccgga tataatttgc ctgacatgcg gccagaagaa agccagcaac
     4741 aatcaatata aaaagaaaaa tagtctttac ctttttcata tactttccct gtatttaaag
     4801 actccggcat tcatccctga acaccggaag tcagtatttc aaatcatcct tgataatttt
     4861 ttgttaccag ttaccgtccc gataatcaaa agctatataa caattatcca catcaggttt
     4921 tatttgattt ttaaaaaatg gctgtagtaa aggaacccat tctttcttca aatcaaactc
     4981 accattatta atattatcct ttaaaggcat tcccaagctt tcaatgacag aaccatcgtc
     5041 accaaagtct ttagaatact cttccccttt aaactcttca gttttcttat caaaccatgt
     5101 caaacgtaat ttaagcccca tatttacctc tcaaagatat tttttaatgt tccgttttgg
     5161 atctggccct ttaacctgct tacccgtttt gggatcaaat gcgcctaggt gttcgccatc
     5221 actggcacga tacccttcaa gctcaccgtg ctgggaatcc cattcataaa ttttacgttt
     5281 tttatcgcca taccatcggg cccgcttacc accaccacct tgtttaggtg ttttaggatc
     5341 acctcgcttt aactctccaa gtccctttat gtcttctgtt ttaggtactg gatgataatc
     5401 atggccgtag tctttagtcc ccttgcgagg cttgttcttt tcctcattta acttattttc
     5461 tgcatccttc gcttttttct ccttttgttt ccggctctcc atcgccccgg taagtgcagc
     5521 cttatctctg gataaggatt cattcattgc gtttaaagat gcctgagccg cttttaattt
     5581 ctcattctga ttctgaatat ccttatcaag atagcgaacc tgatcctgcc acccggagcc
     5641 atattcatga ggcgggttat aagtcacaaa atcatcttta tttgcctctg cctcttttac
     5701 ctttttatcg gcttcctgaa cagcagccct tgcaccgggg agcgccttct ctgtcgatgc
     5761 aatgcggctg tttaaggtcg caatattttt atcttcggca tccagctcag ctttcgcttt
     5821 atcatagtct cttttcagcc cctcttccgg atgagcggcg tcccatgcct gctggcgacg
     5881 cttttcttcc tcctgacgct gttttacctg tgccggggta agaacatctg tcaccgacac
     5941 ataaaccggc ttctgtccgg tctctttcgg gaaacgaata acggcctcac gggagttacc
     6001 accggctgta aaaccagccg gacgtgaatc acctttttca gcaacaatgc cttttggcgg
     6061 ggctttcgct gtcggaacac ctttcggtac gctcacctgc agagacggga gacccggaat
     6121 cgacacactg aatacccccg gacgttttgt cggtttcgca tccaccacag ggacactcat
     6181 tggccgacct gcgacaaccg caatatgctg ccgctcatcc ttcaccacat ccacaacccg
     6241 ttgtctgaca cgaaccgtcg cctggtccag tggtaaagta cttgccggag tctccgtcac
     6301 cgcatcggcc ggtaatgacg tcataatttt tgacatcatt ttcggatcat cttttgcaat
     6361 ctcagaaggc agcacaccgt agatcgcaat cccccacaga ccaaacttaa acggcccctt
     6421 cagggcagcc agcacatcag caacggcggc cgacaacgca tcgccggaaa ctgataaagc
     6481 cagtccttca gcccccggag tagccagggc aggcagaccg aaggccatcg cagtggccga
     6541 agactgtccg ccaccagagc taccgctatg attgccgctg ttaccaccat taccaccggg
     6601 tttactcccg ccgttaccaa actcccccgg attatagtta tgaatatctc cattcggcgt
     6661 atgcgtcgta ccccagcccg gcccgctgtt tggatccgta ccaccacctg atgaagggcc
     6721 acctttacca gacgtaccgt tcacattccc actggcctga ccaccattat gtcccagacc
     6781 tgaattaccc ggacctcgac cgtctccacc actcataccg ttttcctctt tgacatttaa
     6841 aacaataaat taaagccaac atactgtaca tatacacagt ataattatta ccttactcct
     6901 cccgttttca ggtgtcaata tatgagatta gttctcatct tcaggcacaa aaaagcgccc
     6961 ggagccacct gtaagcagat cgcgtctttt cccgaaaatt tctcatggca acaaatttta
     7021 ttatttaaga cattcatcac acttacattt cagatggtat agacacatct gaaagctaac
     7081 tggcatgagg agaggacctg cggttaaact gttcgggaaa cctgagacgc ccggaggcca
     7141 ctatgacgca cctgaaactg actcccgctg aagaaaagcg cctggcggac gcctttgtcg
     7201 ctcaggcact ggccatgggc ctgaaaggga acaaaacgct gccaaaggcg gtaaaacaac
     7261 aggtgcggga aagggtgctt cgtgccattc aggacgaacg tgaagccagg cgggcgcagg
     7321 aaatgcgtcg ggagaaatac gaggctacgg aaaccacctt cacctggcag ccacagcgcc
     7381 gccggtaagg ggtatttacc ataacgtcgc attgcccgca ccgctcagac ggtctgaggg
     7441 cgtttccggc ggctacggga taaaacaggc aacaaaccac ccggaaaacc gccggaaacg
     7501 cgcccggtaa attgtaaccc gctgcatgcc tatgcacgca actgcatggc gggccgtttg
     7561 gtgtaaaaac gatacgataa gccgtgttta tgtaaatgaa cgtggccgta aagagtgaca
     7621 aatatacatg taacattttt tactgacagg tcatcaaccc gatgcgttta atacccttta
     7681 tcctgctggc attgtgcagc cccttactcc tgacggcctg taagcattcc accacccagc
     7741 cgccgaaaat ttatactctg gataagtcat ctaaaatcag tagctacgct gcggccatca
     7801 agaccgctgt tgaaatgcag atgtttgaac cagaggccta tgcgggaaaa atgtgcaccg
     7861 tgcggatatc catacaacgt gatggcactc tgaacagcgc cattgctgaa ggaggagatc
     7921 cgaaactctg taaagcagca atatcagccg ttacccacgc caaaattccg ccggcaccgg
     7981 atgaaaatac ctggcaaata ttcaaaaacg cccctctgga tttcagacca tgaaagcttt
     8041 tactctgaca gtgctggcgg ccatgttcct cactccggta catgcgatac cactaaaacc
     8101 cgtaaccaca ctgcagccaa cctacccggt acaggccgca gcaaaacgca tcgaaggtca
     8161 ggtgaccgcc acgtttgata ttctggacga tggacgagtg gacaacgtgt cgcttaccgg
     8221 ctgtcacctt ttttttcggg aaacgctgac ggcaatgagg cactggctat atcccgcagg
     8281 gcacccggca atgcgacagg ttataaattt tgaattcagt ctgaaaaatg acggcttaca
     8341 ggatccgcga ccagtcacca cccaaaagct caatgatgat ggttgccgcc agtaacaggt
     8401 cctttaaccc gcaggctgca atagtcctgg atgatgagtg agcctgtagc agccctgatt
     8461 agtggaagcg aagctgaaaa atgggcagca aaagccgtat ttctggcaaa tagcgccgcc
     8521 acggattaaa gtagtcatgt gtaatacata tgactacaaa ggggctttta tgtcatcagc
     8581 aacatccatc aggctcgatg aagaacttaa ggaccggctg aaaacgctgg ccgatgaccg
     8641 gcaccgctca gcgcacgcgc taatgctgga ggcaatcacc gaatacatcg accgggaaga
     8701 gaagcgcagc cagtacctgc gggacggaca ggccgcatgg cagcactatc aggaaacggg
     8761 tctgcacctt actgcggaag aagcggaagc ctggataagt acctggggaa ctgagaacga
     8821 gcaggatgct ccgccatgcc acaggtaacg atttccgcgc tggcacagcg cgatttacaa
     8881 cgccttcagg actttctcaa aaccaaaaat cggctggcag ccagaaaagg aggtgaggtg
     8941 atcgtccggg ctatccagca actgaaaaca ttgccagaca ttggccgccc ggtgccgttt
     9001 ctgccgctgg aatataagga actggtgatc gggtttggcg acagtggcta tgtgatgctc
     9061 taccgccacg accgggaaat ggaccagatt gtgatcgtca ccgtcaggca tcagaaagaa
     9121 tccgggtatc cgggggcaga cagcctctaa taaccgatga cgggggcgca attcattgcg
     9181 tggccgtcgc cggtttgaca agcgcagcgc gtcagaaaac cacatcctga acggaaaaac
     9241 gggtaaaatt cagcgataca tttaccgtga gaaattacac acctttcaca cccgataaaa
     9301 tcaggggtat acagtggcga aaacattgct caacctttca caggcagcac aagccgcggg
     9361 cattaccaga agaacgctat acaatcatgt aaaacaggga aaggttaccg tgtcacgtga
     9421 cgggaaaaac aaccctgtcg tggatgtgtc agaactgatc cgtgtatacg gaaatgtgaa
     9481 tatccctgag aaacaaattc ccggcatttc acaccgtgaa aatacacaaa aaaatttccc
     9541 acaggaacag cttctggcaa tgcaaaagga actggccgat ctccggcagg ctgtcaccct
     9601 gatgctggaa gataaaacat cccgggaaga agaacgccgc cagcacgatg atgagcgccg
     9661 gaaactccag gccgaagtgg acagattgac aacagaactg acacagaaga aaaaacgctt
     9721 ctggtccggc tggtttagct gagaacttac cgaagggaat ggagaacatg acgaagcagg
     9781 aaaaaactgc cctcaatatg gctaaattca ttaaggatca gtcgctgctg ttgctggaaa
     9841 agctgaacga gctggacatg gataccccgg ccgacatgtg cgaacggctt cacgaggatg
     9901 cggaaacact ctggcaggtg atgcgggata agctcgggga agaataacag caccggccat
     9961 gccggtgctg gcgttatttt ttgccgaacg gccaccaccg gcgggcgggt ttgtcctccg
    10021 gcagttcagg caccggcacg ttaaaatag
//
