LOCUS       NZ_CP082036             5596 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2172 plasmid pKP18-2172-3,
            complete sequence.
ACCESSION   NZ_CP082036
VERSION     NZ_CP082036.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928638
            Assembly: GCF_019823955.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,635
            Genes (coding)                    :: 5,477
            CDSs (with protein)               :: 5,477
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 58 of 158
            Pseudo Genes (incomplete)         :: 107 of 158
            Pseudo Genes (internal stop)      :: 15 of 158
            Pseudo Genes (multiple problems)  :: 22 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2172"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2172-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-12-24"
                     /collected_by="Zhengzhou University"
     gene            join(5284..5596,1..32)
                     /locus_tag="K6161_RS00005"
     CDS             join(5284..5596,1..32)
                     /locus_tag="K6161_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            241..>675
                     /locus_tag="K6161_RS28830"
                     /pseudo=""
     CDS             241..>675
                     /locus_tag="K6161_RS28830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            710..1219
                     /locus_tag="K6161_RS00015"
     CDS             710..1219
                     /locus_tag="K6161_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1226..1438
                     /locus_tag="K6161_RS00020"
     CDS             1226..1438
                     /locus_tag="K6161_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1814..1942)
                     /locus_tag="K6161_RS28835"
     CDS             complement(1814..1942)
                     /locus_tag="K6161_RS28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            2105..2329
                     /locus_tag="K6161_RS00025"
     CDS             2105..2329
                     /locus_tag="K6161_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2313..2579
                     /locus_tag="K6161_RS00030"
     CDS             2313..2579
                     /locus_tag="K6161_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2649..2999)
                     /locus_tag="K6161_RS00035"
     CDS             complement(2649..2999)
                     /locus_tag="K6161_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3065..3610)
                     /locus_tag="K6161_RS00040"
     CDS             complement(3065..3610)
                     /locus_tag="K6161_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3636..3962)
                     /locus_tag="K6161_RS00045"
     CDS             complement(3636..3962)
                     /locus_tag="K6161_RS00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(4320..4478)
                     /locus_tag="K6161_RS28890"
     CDS             complement(4320..4478)
                     /locus_tag="K6161_RS28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            5118..5294
                     /locus_tag="K6161_RS00050"
     CDS             5118..5294
                     /locus_tag="K6161_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          4953..5032
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4370..4767)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          23..1554
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgctggaaaa gggggcagac gatgatcgtt aagtttcatc cgcgagggcg aggcggcggt
       61 gccgggccgg tggattatct gctggggaaa gaccgccagc gcgacggggc catcgtcctg
      121 cagggaaagc cggaggaggt ccgggagctt atcgacgcct cgccctacgc caaaaagtac
      181 acctccggcg tcctgtcttt tgctgaacag gatttaccgc ccggccagcg tgaaaagctg
      241 atggcgagct tcgagcgggt tctgatgccc ggactcgata aagaccagta cagcgtgctg
      301 tgggttgaac accgggacaa ggggcggctg gaactgaatt ttctggtacc gaacacggaa
      361 ctgctgaccg gcaggcgtct ccagccgtac tacgaccggg cagaccgtcc gcgcatcgat
      421 gcgtggcaga ccgtggtgaa cgggcgactg gggctgcacg acccgaacgc accggagaac
      481 cggcgcgcgc tggtgacgcc atcttcgtta cccaaaacga agcaggaagc cgcagaggct
      541 attacgcggg gcttactggc cctcgcctcg tcaggggagc ttaaaacgcg tcaggacgtc
      601 actgaggcgc tggaaagcgc aggttttgag gtcgtgcgca ccacaaagag cagcatcagc
      661 attgccgacc cggacggagg gcgaaacatc cgacttaagg gagccatcta tgaacagtct
      721 tttaacgctg gcgaaggact tagagcagaa atcgaaagcg cagcagcaga gtaccggcga
      781 aatgctgaaa gccgcattca gcgagcacga gaagtctgtc agagcggaac tgagcgaaag
      841 cgggaagaga atcagcgccg ccatccgcgc ccacgagcag ggtatgagcg cggccatgca
      901 gtcgaaccgc ctgagcgtga tgcgtatggt cgggcggaca tggctgacca tcacgatggt
      961 gtcaggactg ctgttcgcca gcctgagcgg ggtgttgtgg tatcagggga gcttgatagc
     1021 gtcaaatctg gccgaaatcg accggcagaa cgcagcgctg tcgaagctga acgcgaagac
     1081 ctggggcgtg acgtacctgg aagacagcaa cggacgtttt ctggtgctgc cgaaggggac
     1141 ggccgtagac aggacgcaga gctggacggt cgggaacggg cggtcgaagc agaacgcgct
     1201 gagactggtg aaggagtaag tgccgatgac cgagctggaa aaacagttgc tgagcgcatt
     1261 agagcagcta cagcaggact actcgaaaag gctggacgag tgggagagcg ccttcgggga
     1321 atggcggaaa atgtcagggc ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtgac
     1381 gcgcttgagt cagcaggtgg aacgcttgag cgggcaactg cagcgtttga gccggtagtg
     1441 cagcgtcatg agatggccgt ggccgcagaa cgggcgcacg agcaacggca gcacgaaaag
     1501 gaactgacgg aagcgcgttc ccggcagcga agctatgacg gtccgtcgct gggctgaaat
     1561 gcaggaaaac agggttctca tcgggcattt acgctgaaac cggatgtcat tcacctggct
     1621 gcatggctat gcagccaggt aaaatttatc tgtcgcgaac ggggcggttt atcgggtggt
     1681 ttgttgccgg ttttacctga ctgccacccc gttcgcggcg aacccgtccg gggcggtgcg
     1741 ggcaacgggt gttatgttta atcagcgtga acggcagata caaaaaagcc ccggagggct
     1801 tttaactact gacttatttt tttgaagatt tgctgggttt caatttgtca gtatttcccg
     1861 gcgttttttt attgtcacgc tggcgtttat caggttcccc tgttgaatcg gcaattcgtt
     1921 tcggacccgg cttaagaccc ataatcgtta tcctttcaat agttaagagg aaagccctca
     1981 agctagatat attgaccgcg ttacggatta tcaagagggg ctaacaagat tattttttgt
     2041 tcttttaggt tgattgtggt actgttgata tacatgtata gcaaatggtg ttcgggaggt
     2101 ttatatgctt gctatccggt tatctgatga gattgagtcc cgtctggact cgctggcgaa
     2161 gcaaaccggc agaacaaaga cgttttatgc gcgggaagca atactggcgc atctggaaga
     2221 cctggaggat tattatcttt cagcagaaac tgctgcacgc gttcgccgtg gtgatgaagc
     2281 agtgcattcg tctgaagacg tgaggaagtc acttggtctg gacgattaac tattccgatc
     2341 gggcgctcaa atcgttacgc aagatggaca aacagaacgc acgacggatt gtggatttta
     2401 tgagtttacg cattgcagtt gctgccgatc ctcgccagtc agggaagccg ctcaaaggtg
     2461 agctgggcga gttctggcgc tatcgggtgg gagattatcg cgttctgtgt gagatccgag
     2521 atgacgagct tgttatcctt gccgccacga ttggacatcg ccgcgaagtt tacgactgaa
     2581 gcccgctatg cgggcttttt tgatgccgac aacaggtatt atggtaaata ctcgactaac
     2641 tacgtgttct actgtttgtc ggttttagtt aaccagcagg ttgtcttaac gataatgtag
     2701 ccgttatttt taactaataa ggtcatattg ttaacgtcag gcataataaa agacattatg
     2761 gcctcagagt tatcatcaga agcagctctc tgcattacta cttcgattcc cttttctttg
     2821 aatctataag tatccagttg cctattaaac ttagaaagac ctacaaccat acttagtttg
     2881 ttatctttaa cagtaagaga tgcttcctga gtaccattac atcggtagta agcggtgtaa
     2941 ccgtcagcaa aagatcgacc ggggaatata aatgctagga acgcaattaa ccaatacata
     3001 gttgagaatc ttggtattac acatttcact atcctctctt tcatttttcg ttcaccatac
     3061 tttgttatct gatttttaat ttttctattt tataaaccaa gtctctacca aaaaggcgaa
     3121 aattatcctc aaataataat aattgtttaa gaacatccat gaaagcagct gcttcaccct
     3181 cagttatggg agctactcta tataacatat aacctgagtt aagggtttct tttatacttt
     3241 cttcaatgct cattataaat attagtggat tatcattttt cttcccccat gggtttctaa
     3301 ttaatccttc gatgtactca ggtcctttgc gtcctattgc ctgcgcaata ttttttattt
     3361 catctgcgtg agggctttgt tgtacagctt tgtttatgcc tgcttgagtg attttcagag
     3421 actctacacc gtgtatgttg tatgagataa aatgtaataa attaagatgc aatgcaatta
     3481 atccattttt gtttacatct gagtatttgg ataaaaaacg taatgaagtg ttaactctcc
     3541 cttgaagcct aacaattggg agtgctgcct tgtatatttc cttctccgtt aaataccgtt
     3601 taaaaaacat aattctccgc ctccgtatag cgtgcctatt ttatttcgtc gtaaaactta
     3661 gctctgaaat attcctttag aaccatcatg tcattaataa ttgaatctat ctcatccatt
     3721 ttttcttggt attcttttaa tggtgaaggg agcggtaact caatatttga aaagtgattg
     3781 gcgagatagg gatcaaggtt tctcatccat tcaggctttt cgccaatgcg ttcttcaact
     3841 gaaagtgaac ctgataatct ctggttaata agatcactta gactcatttt ggttttattc
     3901 gaaagccgtt gtaaggattg ctttaatcct atatctagca tgatatttgt tctttctctc
     3961 attacccccc cttgctattg cgttttttgt tgcgcacaaa gctaccattg cgcattaaga
     4021 aacgcaataa aaaaggaggg agggagtggt ttgtacatga tgtgcagtgc gctgagggca
     4081 tcagtggcgg gtgtttttcc gtgggatgga tggagctccg gggataacag aactgacgat
     4141 catttaaagg atcttcttga gatccttttt ttctgcgcgt aatctcttgc actgtaaacg
     4201 aaaaaaccgc ctggggaggc ggtttgatcg aaggttaagt cagttgggga actgcttaac
     4261 cgggtaactg ggtatacgag accgcagata ccaaaactgt tctttcagtg tagccgcagt
     4321 taggcaccca cttcaagacc tctcaatatc tctctcgtat atatccagtt accaatggct
     4381 gctgccagtg gcgtttttgc gtgtccgtcc gggttggact caagatgata gttaccggaa
     4441 agggcgcagc agtcgggctg aacggggggt tcgtgcatac agcccagctt ggagcgaacg
     4501 acctaaaccg agccgagata ccagcagcgt gagctatgag aaagcgccac gcttcccgaa
     4561 agggaaaaag gcggaacagg tatccggtaa acggcagggt cggaacagga gagcgcacga
     4621 gggagccgcc ggagagaaat caccggcatc tttaagtcct gtcgggtttc gtcctcctct
     4681 gacttgagcg tccatttctg tgatgctcgt caggggaggc ggagcctatg gaaaaacggc
     4741 aacgccgcat ccttttctcc ggccctttct gatttgagcg cctttgcttt atcccgttat
     4801 ccgtgagtcg gtcacgcccg ccgcagccga acggcaggag cgcagctacc gagtgagcga
     4861 ggaggcgtca tttcatctgt ccgtgcatgg tattgcggac gctcctgagc cacatatcac
     4921 acctgccaca tgaggcactt tcatgaatcg tgctctcatg ccaacatagt aagccagtat
     4981 acacagcgta aacgctgtga ctggttcagg gctgcgcccc gaaacccgct aacaccactg
     5041 acgcgctggc gcttgtccaa cgccggagcg acccggaaaa cttttccgtg ccgtccggcg
     5101 tttgcgaagg agggataatg cagaagccga agaagaagcc gctcgtcgta aaaaaaagat
     5161 cctggcatcc ggcagtgatg gcaaatctga tgcgtcaggc acaggaggaa gattaccgcc
     5221 gggagcagat ggcagagctt gagcgttcag cccggtcaca ttttgcggat atcggaggag
     5281 aggatggcgg ttaagcgtac ccgtttttta ggtatccggg tgaccgatgg ggagtaccag
     5341 cagctgctgg agcgctgcaa tggaaggcaa ctggcggtgt ggatgcggga aacctgtctc
     5401 gatacgcgcc cggcgcggtc gttgcggctg ccgtccatcg atccggtttt gctgcgccag
     5461 cttgccggga tggggaacaa cctcaaccag atcgcccgga aaattaacgg cggccagtgg
     5521 tcaggcgctg accgggttca ggtggtggcc gcgctgatgg ccattgatgc cgaactcgag
     5581 cggctgcggc atgccg
//
