LOCUS       NZ_CP082035            10060 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2138 plasmid pKP18-2138-3,
            complete sequence.
ACCESSION   NZ_CP082035
VERSION     NZ_CP082035.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20928637
            Assembly: GCF_019823875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,678
            Genes (coding)                    :: 5,523
            CDSs (with protein)               :: 5,523
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 62 of 155
            Pseudo Genes (incomplete)         :: 101 of 155
            Pseudo Genes (internal stop)      :: 13 of 155
            Pseudo Genes (multiple problems)  :: 21 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP082035.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2138"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2138-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-12-19"
                     /collected_by="Zhengzhou University"
     gene            complement(join(9831..10060,1..49))
                     /locus_tag="K6163_RS28725"
     CDS             complement(join(9831..10060,1..49))
                     /locus_tag="K6163_RS28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(224..589)
                     /locus_tag="K6163_RS29045"
     CDS             complement(224..589)
                     /locus_tag="K6163_RS29045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(586..957)
                     /gene="tolA"
                     /locus_tag="K6163_RS28735"
     CDS             complement(586..957)
                     /gene="tolA"
                     /locus_tag="K6163_RS28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(1231..1476)
                     /locus_tag="K6163_RS28740"
     CDS             complement(1231..1476)
                     /locus_tag="K6163_RS28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1803..3488
                     /locus_tag="K6163_RS28745"
     CDS             1803..3488
                     /locus_tag="K6163_RS28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3498..3755
                     /locus_tag="K6163_RS28750"
     CDS             3498..3755
                     /locus_tag="K6163_RS28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3840..3989
                     /locus_tag="K6163_RS28755"
     CDS             3840..3989
                     /locus_tag="K6163_RS28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5473..7437
                     /locus_tag="K6163_RS28760"
     CDS             5473..7437
                     /locus_tag="K6163_RS28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7437..8168
                     /gene="mobC"
                     /locus_tag="K6163_RS28765"
     CDS             7437..8168
                     /gene="mobC"
                     /locus_tag="K6163_RS28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            8175..8705
                     /locus_tag="K6163_RS28770"
     CDS             8175..8705
                     /locus_tag="K6163_RS28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8732..8911)
                     /locus_tag="K6163_RS28775"
     CDS             complement(8732..8911)
                     /locus_tag="K6163_RS28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8937..9365)
                     /locus_tag="K6163_RS28780"
     CDS             complement(8937..9365)
                     /locus_tag="K6163_RS28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(9529..9843)
                     /locus_tag="K6163_RS28785"
     CDS             complement(9529..9843)
                     /locus_tag="K6163_RS28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     BGC             1..8488
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4093..4671)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7438..8165
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gccggtcctt aagttcttca tcgagcctga tggatgttgc tgatgacata aaagcccctt
       61 tgtagtcata tgtattacac atgactactt taatccgtgg cggcgctatt tgccagaaat
      121 acggcttttg ctgcccattt ttcagcttcg cttccactaa tcagggctgc tacaggctca
      181 ctcatcatcc aggactattg cagcctgcgg gttaaaggac ctgttactgg cggcaaccat
      241 catcattgag cttttgggtg gtgactggtc gcggatcctg taagccgtca tttttcagac
      301 tgaattcaaa atttataacc tgtcgcattg ccgggtgccc tgcgggatat agccagtgcc
      361 tcattgccgt cagcgtttcc cgaaaaaaaa ggtgacagcc ggtaagcgac acgttgtcca
      421 ctcgtccatc gtccagaata tcaaacgtgg cggtcacctg accttcgatg cgttttgctg
      481 cggcctgtac cgggtaggtt ggctgcagtg tggttacggg ttttagtggt atcgcatgta
      541 ccggagtgag gaacatggcc gccagcactg tcagagtaaa agctttcatg gtctgaaatc
      601 cagaggggcg tttttgaata tttgccaggt attttcatcc ggtgccggcg gaattttggc
      661 gtgggtaacg gctgatattg ctgctttaca gagtttcgga tctcctcctt cagcaatggc
      721 gctgttcaga gtgccatcac gttgtatgga tatccgcacg gtgcacattt ttcccgcata
      781 ggcctctggt tcaaacatct gcatttcaac agcggtcttg atggccgcag cgtagctact
      841 gattttagat gacttatcca gagtataaat tttcggcggc tgggtggtgg aatgcttaca
      901 ggccgtcagg agtaaggggc tgcacaatgc cagcaggata aagggtatta aacgcatcgg
      961 gttgatgacc tgtcagtaaa aaatgttaca tgtatatttg tcactcttta cggccacgtt
     1021 catttacata aacacggctt atcgtatcgt ttttacacca aacggcccgc catgcagttg
     1081 cgtgcatagg catgcagcgg gttacaattt accgggcgcg tttccggcgg ttttccgggt
     1141 ggtttgttgc ctgttttatc ccgtagccgc cggaaacgcc ctcagaccgt ctgagcggtg
     1201 cgggcaatgc gacgttatgg taaatacccc ttaccggcgg cgctgtggct gccaggtgaa
     1261 ggtggtttcc gtagcctcgt atttctcccg acgcatttcc tgcgcccgcc tggcttcacg
     1321 ttcgtcctga atggcacgaa gcaccctttc ccgcacctgt tgttttaccg cctttggcag
     1381 cgttttgttc cctttcaggc ccatggccag tgcctgagcg acaaaggcgt ccgccaggcg
     1441 cttttcttca gcgggagtca gtttcaggtg cgtcatagtg gcctccgggc gtctcaggtt
     1501 tcccgaacag tttaaccgca ggtcctctcc tcatgccagt tagctttcag atgtgtctat
     1561 accatctgaa atgtaagtgt gatgaatgtc ttaaataata aaatttgttg ccatgagaaa
     1621 ttttcgggaa aagacgcgat ctgcttacag gtggctccgg gcgctttttt gtgcctgaag
     1681 atgagaacta atctcatata ttgacacctg aaaacgggag gagtaaggta ataattatac
     1741 tgtgtatatg tacagtatgt tggctttaat ttattgtttt aaatgtcaaa gaggaaaacg
     1801 gtatgagtgg tggagacggt cgaggtccgg gtaattcagg tctgggacat aatggtggtc
     1861 aggccagtgg gaatgtgaac ggtacgtctg gtaaaggtgg cccttcatca ggtggtggta
     1921 cggatccaaa cagcgggccg ggctggggta cgacgcatac gccgaatgga gatattcata
     1981 actataatcc gggggagttt ggtaacggcg ggagtaaacc cggtggtaat ggtggtaaca
     2041 gcggcaatca tagcggtagc tctggtggcg gacagtcttc ggccactgcg atggccttcg
     2101 gtctgcctgc cctggctact ccgggggctg aaggactggc tttatcagtt tccggcgatg
     2161 cgttgtcggc cgccgttgct gatgtgctgg ctgccctgaa ggggccgttt aagtttggtc
     2221 tgtgggggat tgcgatctac ggtgtgctgc cttctgagat tgcaaaagat gatccgaaaa
     2281 tgatgtcaaa aattatgacg tcattaccgg ccgatgcggt gacggagact ccggcaagta
     2341 ctttaccact ggaccaggcg acggttcgtg tcagacaacg ggttgtggat gtggtgaagg
     2401 atgagcggca gcatattgcg gttgtcgcag gtcggccaat gagtgtccct gtggtggatg
     2461 cgaaaccgac aaaacgtccg ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc
     2521 aggtgagcgt accgaaaggt gttccgacag cgaaagcccc gccaaaaggc attgttgctg
     2581 aaaaaggtga ttcacgtccg gctggtttta cagccggtgg taactcccgt gaggccgtta
     2641 ttcgtttccc gaaagagacc ggacagaagc cggtttatgt gtcggtgaca gatgttctta
     2701 ccccggcaca ggtaaaacag cgtcaggagg aagaaaagcg tcgccagcag gcatgggacg
     2761 ccgctcatcc ggaagagggg ctgaaaagag actatgataa agcgaaagct gagctggatg
     2821 ccgaagataa aaatattgcg accttaaaca gccgcattgc atcgacagag aaggcgctcc
     2881 ccggtgcaag ggctgctgtt caggaagccg ataaaaaggt aaaagaggca gaggcaaata
     2941 aagatgattt tgtgacttat aacccgcctc atgaatatgg ctccgggtgg caggatcagg
     3001 ttcgctatct tgataaggat attcagaatc agaatgagaa attaaaagcg gctcaggcat
     3061 ctttaaacgc aatgaatgaa tccttatcca gagataaggc tgcacttacc ggggcgatgg
     3121 agagccggaa acaaaaggag aaaaaagcga aggatgcaga aaataagtta aatgaggaaa
     3181 agaacaagcc tcgcaagggg actaaagact acggccatga ttatcatcca gtacctaaaa
     3241 cagaagacat aaagggactt ggagagttaa agcgaggtga tcctaaaaca cctaaacaag
     3301 gtggtggtgg taagcgggcc cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg
     3361 attcccagca cggtgagctt gaagggtatc gtgccagtga tggcgaacac ctaggcgcat
     3421 ttgatcccaa aacgggtaag caggttaaag ggccagatcc aaaacggaac attaaaaaat
     3481 atctttgaga ggtaaatatg gggcttaaat tacgtttgac atggtttgat aagaaaactg
     3541 aagagtttaa aggggaagag tattctaaag actttggtga cgatggttct gtcattgaaa
     3601 gcttgggaat gcctttaaag gataatatta ataatggtga gtttgatttg aagaaagaat
     3661 gggttccttt actacagcca ttttttaaaa atcaaataaa acctgatgtg gataattgtt
     3721 atatagcttt tgattatcgg gacggtaact ggtaacaaaa aattatcaag gatgatttga
     3781 aatactgact tccggtgttc agggatgaat gccggagtct ttaaatacag ggaaagtata
     3841 tgaaaaaggt aaagactatt tttcttttta tattgattgt tgctggcttt cttctggccg
     3901 catgtcaggc aaattatatc cgggatgttc agggcggaac ggtggcacca tcgtcctcct
     3961 ctgaactgac ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt
     4021 ttttctgcgc gtaatctttt gccctgtaaa cgaaaaaacc acctggggag gtggtttgat
     4081 cgaaggttaa gtcagttggg gaactgctta accgtggtaa ctggctttcg cagagcacag
     4141 caaccaaatc tgtccttcca gtgtagccgg actttggcgc acacttcaag agcaaccgcg
     4201 tgtttagcta aacaaatcct ctgcgaactc ccagttacca atggctgctg ccagtggcgt
     4261 tttgcgtgct ttcccgggtt ggactcaagt gaacagttac cggataaggc gcagcagtcg
     4321 ggctgaacgg ggagttcttg cttacagccc agcttggagc gaacgaccta caccgagccg
     4381 agataccagt gtgtgagcta tgagaaagcg ccacacttcc cgcaagggag aacggcggac
     4441 aggtatccgg taaacggcag ggtcggaaca ggagagcgca agagggagcg acctaccgga
     4501 aacggtgggg atctttaagt cctgtcgggt ttcgcccgta ctgtcagatt catggttgag
     4561 cctcacggct cccacagatg caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg
     4621 gcggagccta tggaaaaacg ccaccggcgc ggccctgccg ttttgcctca catgttagtc
     4681 ccctgcttat ccacggaatc tgtgggtaac tttgtatgtg tccgcagcgc ccgccgcagt
     4741 ctcacgcccg gagcgtagcg accgagtgag cgaggaggcg caatttcccc ggcacctgca
     4801 agacggtcac cggacgcgct gatgtgggat attgtcccct gattaagtct ttctattccc
     4861 tgacgatcaa ttatcaggat aggtgaacca tgtcggtttt tcgctgaaca ttttacgtta
     4921 acctatctct gttgcttttt tctccttgcg gagaaatgca agtcatcccc gctggggcgt
     4981 ggctgacttc aggggaaaag accttgctta cgctggctgg acgccaccga cgcacggtct
     5041 tttcccctct ttctggctct gtccggctgg acgccggcca cgccagaaat tcacccggcc
     5101 cccgtcaggc tcacgcctga ccacccacaa aaatccacgg tgctggacgc acctgacgaa
     5161 gcagcaaagc tgctcctgat tttgcgggtg gtcagccgct cgctatggcg aactctgacg
     5221 ggggccggaa caccggaaaa gaccgcactg gggtgccgcc ttttcctctc acacgctggg
     5281 gcgtgctcgt tgttcctccg gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc
     5341 tccgcggtga aagctgaacg caatcaccac ggacgtgacc gccgccgcca tgccttgggg
     5401 catgtccgcg gcttgtgcag cgggggctgc gcatcacgcc ggtaaacccg tcgtctgaag
     5461 gagagagatt gcatgtttaa tacggattcg cttgcctggc agtggcatat cccggagctg
     5521 gcccggcaga tttacccgtg ggtcagctgg ctgtaccggg agtattacag tctggccggt
     5581 ttttttctgg ttatcggcct gtttttcggt ctggctgaac tctgggccgg ccgaatgcgt
     5641 tttgtggggc tgagccataa ggcgcggcgg gagtatgccg atcgctataa gcgcgataat
     5701 aaccggactg tgccattttc ggtgctggcc tcgctggatt cacccgggcg gatggtcagg
     5761 gcacggcgac atgtctggaa ctacgcgtac tggctgaccg gcgctgccgg tctggccctt
     5821 ctgggggggc tggttgtcgc cgtgtttatc gagagctggg gagaaaccgt cctgcgcccg
     5881 gacctctatg cgaacgcaga ttattacctg ctgcagccgg ctaacctgtt cgggatagcc
     5941 tggtgtgccg gagggcttgt cgccggcgtc cttctggcgc ggctgctggc gttcctgctg
     6001 cttgatggtc attttgcggc cgctgacgag gcggtgaacg cgaaactgaa tcaggagagc
     6061 cggcgcagca gccagcggac cggagaaatg acagacgtcc gccacctgca tttcggggag
     6121 ccggtaccgg ttaacgcgct ggccgatttt tccacggagc aggcccggaa acagcaggcg
     6181 gtgtttctgg gtaaggatga gcagggccag ccggtgctgg tccctcgtga tacctggcgc
     6241 aagaccaata ttcagatcct cggcctgccc gggagcggca agagcgcgat ggccaccaac
     6301 gcgctcattc gctgcgtacg cgatttcggg gacgctgtgg tgtacttcga ccccaaaggg
     6361 gacgcgtggg cgccgcacgt cttccgggcg cactgcccgg acttcaccct gctggatctg
     6421 cggccgggga aaccggcgca gctgaatctg ttccgcgatc tcgaccagta cgccctgaag
     6481 aatctgctgg ttgccgggtt taacctgagt gagaccggcg acgtggcgga ccactaccgc
     6541 atcagcgagc agaaagcggc gaagctgatt gcggagcagt tcccgcaggg ggcaaacatt
     6601 cagcaggtgc tggcggcggc gtacgcgttg ccggaggccc tgaaaaagga cgtgaagggg
     6661 ctgatcacca agctggagaa cgtggccgac ctgagcgtcc tgcagacgga cagcggtatc
     6721 gatgtggcgg ggataatcaa cggcggcggc tgcctgtacg tcatcggctc gatggacgat
     6781 gaggcggtga tccgggtgca gaagatgctg tttgcccgct gcgcgcaaat catcattgcg
     6841 cgggatgagt tccggaggtg gccgcacgcg agcatcatgc tcgatgagat taaatatctg
     6901 ctgtcgaagt acgtgctgaa cgcgctgggg acgctgcgca gccgggactg caacctgctg
     6961 ctcgcgcacc agtcgctggg cgacttcggt cagtgcgggc aggatctgcc cgctgatttt
     7021 gtgaaaacca cggtactgga taacacgccg atccgctggt tctatcgcgc ggccagccag
     7081 gagtccgcgc agtgggcggc gggccagacc ggggagatcc gggtggacgt ggagcgccgc
     7141 cgggccagcc gggaggcggg gaacgtggag cacatcagcg gcgacagctt catccagaag
     7201 gaggcgcggc cgctgtttga cgtgaacacc ctgcaacacc tgcctgacgg gttcgcggtg
     7261 atgaccgggc tgggcgtggc gcggctggcg ttcagcagcc cgctgcgggt tgagcgccgg
     7321 gaaatttcgc tgaaaacctt cccggtgctg gcgaaaacgg acccgctggc ggagtacctg
     7381 cctccggccg acggcggcgg accaaaagcg gatgatgatt ttgcggggct ttactgatga
     7441 cactggagca gtacaacgtc agccacgcga agcggcaggc gcggaacgcg gagaaaacac
     7501 ggctgaccct gcgctggctg cgggaagagc tgtgttccac ggcggagctg gttgcgcgac
     7561 ggctggggat tgcggcggta cagccggttt accgcttcct ggacagtctg gtggcgaaag
     7621 ggctgctggt ccgggcgaaa tacccggttg acggccgtca ggtcagcgtg tgggggctga
     7681 cgccgcacgg ggtggcgttc tcgttcgacg aggacgagcc gctgacggac gttattccgt
     7741 ttcagccgtc aagggtgtcg gcggcgcagc tgccgcaccg gctggccgtg cagtccctgc
     7801 gcctctccat ggagggccgg ggggccaccg gctggcggta cctgcaccgt atggccctga
     7861 aagggatgaa agtgccggac gcgctggcgg agctggacgg cagaacggtg gcatttgagg
     7921 tagagcgcac cgtcaagagc cgcaggcggt atcaggaagt ggtgtccggg tatctgttca
     7981 accgccgggc gaacggcatc gatgaaatct ggtacatctg ccctgaccgg ccgacgcttg
     8041 tccgggtaca gcgggcgatt ctggcggtgg atgaaatcgt gaatccacag accggcgagg
     8101 cccgcaaaac ggtggagctg gaccgggaga ggctgttcgc gtgctttaaa tttatgacaa
     8161 cggagtgagg gattatggcg ctgtacatgg tatttattga ggacgtggaa gacaaagacg
     8221 gcgagcgctg cattatgcca accagcgagc atctggaaga catggtggtg ctcgcctggc
     8281 agaccggggc gtatgttttc tgttttgtcg accgggccca gcatctggcg atgatgaaat
     8341 ccatcgggat gtatgacgag cggctcaaca gcgacgaaaa agccacgtac tttttcaccg
     8401 gtgaaaatcc gcaatgggaa cgggacaaac ccacaggaat gaagctgtgt gattacctct
     8461 atcgaaaggt ggagcggttc gccatcgaga acggtgtgca ggtggttgat gccgggaagt
     8521 gctcaccgga gcagattaac gcgatgattg aggtggtcag cgagcggcac ggggaaagcg
     8581 cggtgaacga gaagctgaaa gcggagctga tgcgggaata taacagccgc tattttaacg
     8641 tgccggtgcc tgaactgccg gaggacaaac ccgcccgccg gtggtggccg ttcggcaaaa
     8701 aataacgcca gcaccggcat ggccggtgct gttattcttc cccgagctta tcccgcatca
     8761 cctgccagag tgtttccgca tcctcgtgaa gccgttcgca catgtcggcc ggggtatcca
     8821 tgtccagctc gttcagcttt tccagcaaca gcagcgactg atccttaatg aatttagcca
     8881 tattgagggc agttttttcc tgcttcgtca tgttctccat tcccttcggt aagttctcag
     8941 ctaaaccagc cggaccagaa gcgttttttc ttctgtgtca gttctgttgt caatctgtcc
     9001 acttcggcct ggagtttccg gcgctcatca tcgtgctggc ggcgttcttc ttcccgggat
     9061 gttttatctt ccagcatcag ggtgacagcc tgccggagat cggccagttc cttttgcatt
     9121 gccagaagct gttcctgtgg gaaatttttt tgtgtatttt cacggtgtga aatgccggga
     9181 atttgtttct cagggatatt cacatttccg tatacacgga tcagttctga cacatccacg
     9241 acagggttgt ttttcccgtc acgtgacacg gtaacctttc cctgttttac atgattgtat
     9301 agcgttcttc tggtaatgcc cgcggcttgt gctgcctgtg aaaggttgag caatgttttc
     9361 gccactgtat acccctgatt ttatcgggtg tgaaaggtgt gtaatttctc acggtaaatg
     9421 tatcgctgaa ttttacccgt ttttccgttc aggatgtggt tttctgacgc gctgcgcttg
     9481 tcaaaccggc gacggccacg caatgaattg cgcccccgtc atcggttatt agaggctgtc
     9541 tgcccccgga tacccggatt ctttctgatg cctgacggtg acgatcacaa tctggtccat
     9601 ttcccggtcg tggcggtaga gcatcacata gccactgtcg ccaaacccga tcaccagttc
     9661 cttatattcc agcggcagaa acggcaccgg gcggccaatg tctggcaatg ttttcagttg
     9721 ctggatagcc cggacgatca cctcacctcc ttttctggct gccagccgat ttttggtttt
     9781 gagaaagtcc tgaaggcgtt gtaaatcgcg ctgtgccagc gcggaaatcg ttacctgtgg
     9841 catggcggag catcctgctc gttctcagtt ccccaggtac ttatccaggc ttccgcttct
     9901 tccgcagtaa ggtgcagacc cgtttcctga tagtgctgcc atgcggcctg tccgtcccgc
     9961 aggtactggc tgcgcttctc ttcccggtcg atgtattcgg tgattgcctc cagcattagc
    10021 gcgtgcgctg agcggtgccg gtcatcggcc agcgttttca
//
